User`s manual - Frank`s Hospital Workshop
Contents
1. Linearity limit Linear limit The maximum measuring limit is given in the reagent technical sheet If result is beyond this limit it is recommended to dilute the Jalue 52 sample or rerun the test CAUTION in hyper active samples having decreasing kinetic type of reaction total substrate can be consumed before first measure starts Shown by reading result close to zero below the depletion limit It is user s responsibility to interprete reading result taking into account this element Normal high Normal High amp Low values border the acceptable pathological range It is also called the usual expected values Flags will be shown on abnormal result Normal high Value 34 88 Normal high Normal low Value 0 db Normal low CHECK FREQUENCY At the end of programming you are asked the frequency of analysis quality control checking whether daily or periodically A signal will remind you to do the quality control every day or once every x days Result of the QC can be found at lt CONFIGURATION gt lt QUALITY CONTROL gt and you can ask for lt DISPLAY gt 40 BASIC 8 2 RECOMMANDATION FOR DAILY ANALYSIS Zero measurement will be asked at the beginning of each day Zero Measurement istilled water ge Zero measurement Distilled water Introduce tube to the noozle upon instructed by display Audio beeper indicates aspiration Remove tub2. 208 So A See As soon the standards read the curve will displayed Absorbance values of standards also displayed By validating F6 lt OK gt the values are stored in memory 8 3 ERROR MESSAGES ALCOHOL 37 Readent Elk 27 ALBUMIN 5 8 9 sam 9 0 9 sam l HA XE Error messages Sample absorbance value is superior to 2 5 above the reading limit of BASIC in this case the software will show lt gt Check any possible manipulation errors the sample not prepared correctly or the cleanlinest of the cuvette The result in Concentration is superior or inferior to the normal high or low value In this case a sign lt gt Or lt gt gt will be displayed following the result Negative Concentration value will be indicated by the sign The result in Concentration is no longer in the linearity limit of the analysis another sign lt lt gt shows up in front of the result in this case the result is also beyond the normal high or low range During decreasing kinetics method if the difference of Optical Density between the initial O D at T0 and at T1 end of the latent time is superior to the depletion limit the software does not display any result 43 BASIC If the initial O D value of the blank is superior or inferior to the limit i a warning sign appears following the concentration
3. Asat GOT HCG LH MO MI M12 M29 M30 M ME HDL Choles LDL Choles Creatinine Alat GPT Ck NAC Progesterone Prolactine M3 MIA 15 M31 M32 M43 NN rom GT Amylase Apo A Glucose Iron Magnesium M MIZ M33 M34 M45 RBC Magnesium Phos Lipids B eol M9 M21 T Protein Triglycerides Urea ME Uric Acid Lipase Nucleotidase 16 BASIC e BASIC s software control keys Validate operation g ese Conf Reach software configuration screen amp correct data 6 3 DISPLAY DESIGN Test name Programmed temperature Concentration value Standard Absorbance value blank value deducted Name of sample measured standard sample X When using cuvette by cuvette method you will see Calculating method 17 BASIC SET UP 7 1 GENERAL SET UP XE General set up Step by step set up of BASIC is done through the lt CONF key CLOCK Use F1 to F5 keys to modify the values Abort modification with ESC key register with VAL key STAND BY 2 E Access to a sub menu allowing to choose the duration before starting the stand by screen saver DATA FILES Access to Patient identification Patient report Data archiving PRINTER Access to set up menu for the printer to PC or Printer Memory reset 19 BASIC PROGRAM PUNP Access to program
4. BASIC 70VB0358 70VB0370 User s manual Ref 0NVBAU Manual version 1 19 4 SYSTEME QUALITE CERTIFIE N MOA SECOMAM a NOVA ANANLYTICS Company amp ANALYTICS 91 Avenue des Pins d Alep NF EN ISO 9001 2000 cofrac 30319 ALES FRANCE Tel 33 4 66 54 35 60 CERTIFICAT 2003 CLXV Fax 33 4 66 54 35 69 CERTIFICATION E mail info secomam fr En EPRISES ACCREDITATION 4 016 98 amp DE PERSONNELS URL www secomam fr BASIC T ERENARK5 APA u u uu 2IWARRAN ZE uuu cos Y INFORMATION 22222 o E N AR A PRECAUTIONS OE USE uuu unus ananas uuu A i S QS uu O DESCRIPTION 52 aba uuu aa huu aaa a 6 1 GENERAL OVERVIEW 6 2 SCREEN AND KEYBOARD 6 3 DISPLAY DESIGN iib 7 1 GENERAL SET UP 7 2 LANGUAGE SET UP 7 3 DATE AND CLOCK SET UP 7 4 PUMP SET UP 7 5 PRINTER SET UP 7 6 TRACEABILITY SET UP 7 6 1 Downloading to a PC F1 lt BASIC PC 7 6 2 Downloading to a printer F2 BASIC PRINTER gt 7 6 3 KINETICAL REPORT 7 7 QUALITY CONTROL SET UP 8 PROGRAMMA TION 8 1 PROGRAM KEYS 8 2 RECOMMANDATION FOR DAILY ANALYSIS 8 3 ERROR MESSAGES 8 4 SAMPLE NUMBERING OD B ES ngon p Qf 9 1 TECHNICAL CHARACTERISTICS 1O MEMO u u TIGEOSSAR Y iaa iS uis um Mh eet be a BASIC 1 REMARKS
5. The Automatic aspiration system kit is manufactured by SECOMAM The information contained in this document may be the object of patents or patent applications by SECOMAM The possession of this document does not confer any license to such patents The following names are SECOMAM s registered trademarks throughout the world SECOMAM BASIC This manual is updated periodically The updates are included in the new editions All information supplied in this edition of the manual may be amended before the products described herein are available All reproduction rights reserved Reference Date oflast Version Date of document Writer Controller Approving modification BASIC 2 WARRANTY The new equipment and material sold by SECOMAM is guaranteed against any manufacturing defects for one year unless otherwise stated by SECOMAM with effect From the technical acceptance of the equipment in the factory by the buyer or his designee or failing this For Metropolitan France from the date on the delivery note For other destinations from the date of factory shipment certified by air waybill consignment note or bill of lading The SECOMAM company guarantee applies exclusively to defectiveness arising from a design fault or from a concealed defect It is strictly limited to the free dispatching of replacement parts except for consumable items or to the repairing of the equipment in our workshops within a deadline of 10 w
6. When dangerous substances for health and environment are used the laboratory or site rules where the instrument is installed must be followed Take all the necessary precautions during the use the instrument to protect the operator from eventual liquids leaks or spills or possible radiations protective gloves anti radiation glasses protected clothes etc All operations made inside the instrument must be done by SECOMAM or by SECOMAM s authorized technicians 11 BASIC 5 PRELUDE The BASIC was designed within the framework of Vitro Diaenostic to cover the need of semi automatization in clinical biochemistry analysis It includes the principal functions required by this kind of analysis such as Wavelength range 340 700 nm Peltier thermostated cell holder Calculation method adapted to enzymatic reading of results and parameters Quality control management Partial management of laboratory practice BASIC complies with the requirements in CE marking of medical devices for In Vitro Diagnostics 98 79 EEC 13 BASIC 6 DESCRIPTION 6 1 GENERAL OVERVIEW Front view XE Front view 1 lt Program keys number keys T Control keys Screen gt Function keys Cursors XE Screen H XE Keyboard 6 2 SCREEN AND KEYBOARD Function screen __keys__ Thanks to these keys working with BASIC is simplified Screen DES instructions as the Distill
7. control blank Column 2 Sample number Colonne 3 Concentration value and unit Column 4 Date and time Column 5 Status No problem Result superior to normal value Result inferior to normal value result superior to linearity limit negative concentration value Blank value superior to linearity limit Control non performed Control badly performed Control normal V uM Memory initialisation XE Memory initialisation To erase the results stored in memory press init 27 BASIC 7 6 3 KINETICAL REPORT On methods using KINETICS you have a choice of having a short one line final reading report by choosing F5 lt NO gt or segment per segment reading through F4 lt YES gt 7 7 QUALITY CONTROL SET UP CONFIGURATION PROGRAM AUALITY Each Quality Control runned is memorized automatically F4 NO gives you the choice not to run F3 DISPLAY allows to display on screen as graph the values and dates of previous control only after at least two controls of the same analysis method have been made Printout of can be obtained after programming TRACE ABILITY function YES XE Quality control set up Quality control 3 ALBUMIN PT Check analysis ANALYSIS and flags will be given according to the MAXIMUM amp MINIMUM TOLERANCE VALUES entered Q preceding result indicates that QC was not performed Upon validating F1 Y
8. calibration Prepare ahead four test tubes with 2000 ul of distilled water each e one test tube with 1000 ul of distilled water to use as standard e One graduated pipette vol up to 1000 ul To adjust pump volume you need to XE Pump calibration e Return to lt CONFIGURATION gt e Goto menu PUMP e Use pump YES Exitfrom lt CONFIGURATION gt ESC twice Rince twice consecutively using right cursor button to fill the tube with water Then e Again return to lt CONFIGURATION PUMP YES PUMP RATE gt e Introduce the first tube containing 2000 ul of distilled water to the aspiration noozle e Press lt SUCK gt and lt VALidate gt the message of 1000 ul The pump should have aspirated 1000 ul Compare the remaining volume with the volume of standard tube Or better yet aspirate the remaining distilled water with the pipette and verify the volume If the remaining volume is more or less than 1000 ul DELete the message of 1000 ul on display and enter the real volume sucked by the pump using the number keys calculation example initial volume of 2000 ul remaining volume new volume to program Introduce the second tube containing 2000 ul of distilled water e Press lt SUCK gt and lt VALidate gt The remaining volume should be 1000 ul same as the volume of standard tube or verify again with the pipette If another adjustment is to be done
9. first order kinetics This analysis method allows to perform measurements of Concentration in Kinetics mode That is to calculate the absorbance variation of a solution within the time and to multiply the differences of Absorbance by a coefficient or to compare to a standard measured with the same measuring conditions Thus global waiting time of measurement should be indicated Then BASIC requires confirmation on the time consumed between the two point reading 972 BASIC R ABS t1 ABS t2 x factor x 60 im 5 11 Abs of the solution at the end of the lag time ABS 12 Abs of the solution at the end of the measuring time factor Coefficient of molecular absorption tm Measuring time 60 tm Ratio reducing the result to one minute The slope thus calculated is expressed in delta of Abs min Reading is done using 2 wavelengths multiplied by a factor F5 Bichromatism Bichromatism is recommended when sample is cloudy turbid IN Abs Smpl Abs Smpl F 38 BASIC For both KINETIC modes enter Lag time the waiting time before reaction starts number of intervals and time segments of each interval Indicate also whether it is an increasing decreasing type of reaction Analysis made fi Humber of intervals Value 5 2 1n seconds 3 d 4 mn Interval time Value 10 1n seconds reactlon Directian 39 BASIC Linearity limit
10. repeat this process untill the instrument aspirated the right 1000 ul volume Always validate at the end of process by 6 To keep previous volume press lt ESC gt Delay Is the frequency in seconds to program for sample introduction following one reading You can work faster by shortening pump aspiration delay when you are more familiar with the system Delay before measure 22 BASIC beginninq of the dead volume Dead volume Dead volume is needed to avoid contamination of samples It is represented by a certain volume of air Dead volume is influenced by the length of aspiration tube It is recommended to program dead volume in such a way that the end of the aspirated sample remains approximately 2 cm on top of the cell tip see drawing XE Dead volume Flow through cell Upon selecting F3 Dead Volume you will get a choice of 6 volumes 4 XE Volume 200 ul is the factory preprogrammed volume 7 5 PRINTER SET UP XE Printer set up CONFIGURATION Select lt PRINTER OPTION gt by pressing F3 Printer should be an 80 column or has an 80 column set up When using a printer select F2 lt YES gt F1 lt TYPE gt enter the type of printer A selection of 4 printers are proposed KYOLINE CITIZEN IDP562S HEWLETT PACKARD and O ES Tyre CO ues O C Connection EPSON Choose one Next check the ty
11. CONFIGURATION modification j l a up menu QUALITY CONTROL WES k HO Qualita control Confirm F1 or skipping F4 the quality control function Display F3 values and dates of previous control NOTE CONFIGURATION screen is active when you see an elevator in the middle of the screen Use upward and downward cursor keys to activate have access to the next display screen Elevator 7 2 LANGUAGE SET UP This would be the first configuration to check upon starting the instrument Successively press CONF gt lt downward cursor key gt F1 lt Language set up gt then lt English gt XE Language set up 20 BASIC 7 3 DATE AND CLOCK SET UP Woe 294199 18 26 am Use the corresponding function key to increment date and time Then VAL to XE Date set up XE clock set up store ESC to return to previous display 7 4 PUMP SET UP XE Pump set up Access to pump functions amp set up debit dead volume preadjusted IT aspiration volume 1 For your convenience these volumes are factory Note upon changing pump and tubings you have to PROGRAM QUALITY beaver SENNY Press F5 NO for manual cuvette by cuvette operation recommended to use on highly contaminant tests By answering F2 YES you obtain three choices 21 BASIC Pump rate
12. l 30 BASIC SECOMAM Polyphotometer UV Vis BASIC V1 11 Date 05 06 1997 Hour 09H26 User 274 day Analysis ALBUMIN Abs Reagent Bl 0 000 0 00 g l Contr le 2 100 21 00 g l Control Pb Le 06 06 97 Normal value 38 00 0 100 1 00 g l Ls 600 16 00 g l SECOMAM Polyphotometer UV Vis BASIC 1 11 Date 06 06 1997 Hour 09H27 User 3 day Analysis ALBUMIN Reagent Bl 0 000 Control 0 990 PB Control 0 990 PB Control Pb Le 07 06 97 Normal value 38 00 54 00 g l Status gt Sample concentration superior to upper limit lt Sample concentration inferior to lower limit PB Problem on control OK satisfactory control Q Appears next to analysis result when control is programmed but not runned Appears next to analysis result when the control is out of tolerance range L Theresult in concentration is no longer within the linearity limit of the analysis The result in concentration is negative Upon modifying analysis method memorized results are erased 31 BASIC 8 PROGRAMMATION Through configuration select lt PROGRAMMING gt to modify a preset analysis method or to create new one Programmable parameters are suction volume temperature unit accuracy calculation mode analysis mode time lag time interval time and number of intervals wavelength linearity limit n
13. result T ALCOHOL FL If the Quality 1 value is beyond the specified limit the sign lt appears in fr nt of the Concentration result In case of Peltier failure Tsign appears instead of the temperature ih BBB gt programmed Ech 1 1 LAR PATIENT REPORT 4 PRINT OUT PATIENT REPORT Daily analysis are memorized automatically by BASIC and indicated at the bottom of the result display as a blinking line User is recommended to empty the memory at the end his working session This can be done transferring all data to print see chapter 5 6 2 or downloading all data to file on aPC see chapter 5 6 1 or selecting particular data to print see chapters 5 6 2 amp 5 6 3 In this case remember to use patient number as selection criteria Note the latest of sample bearing identical number will be reported Print out When quality control is programmed error messages will be given on lt status gt column SECOMAM Polyphotometer UV Vis BASIC V1 11 Date 04 06 1997 Hour 09 24 User Analysis ALBUMIN 37 u Regeant Bl Standard Control 44 Date 04 06 1997 Hour 09 24 User Analys s ALBUMIN Reagent Std Control Control Control S S S When everything goes well OK will be indicated SECOMAM Polyphotometer UV Vis BASIC V1 11 Date 04 06 1997 Hour 09H24 User Analysis ALBUMIN Reagent Sample Control 8 4 SAM
14. the return transport of the equipment falls within the framework of the guarantee on the express condition that the customer has sent his complaint within forty eight hours by registered letter with acknowledgement of receipt to the carrier copy of the letter should be sent to SECOMAM For equipment with a guaranty card this is only applicable if the card delivered with the equipment is returned to SECOMAM duly completed BASIC SOFTWARE GUARANTEE The software is guaranteed by the designer or the distributor of the software under the conditions specified in the literature accompanying the aforementioned software packages Under no circumstances whatsoever will SECOMAM supply any type of guarantee for software packages By express agreement all damages notably for staff costs lost of earnings business trouble etc are strictly excluded from our guarantee The customer is informed that the software cannot be guaranteed exempt from defects or bugs TRADE SECRET AND PROPERTY RIGHTS This document is protected by a SECOMAM copyright c 2003 and the copying rights are explicitly reserved The software supplied with the equipment or referenced contains trade secrets and confidential information which are SECOMAM s property It is legally protected by the international copyright c laws SECOMAM grants a license to use its software to the user This may not be disclosed used or duplicated with the intention to save it without SECOMAM s
15. ES you will be reminded to run Quality Control later on upon running a test method CHECK Note Upon using a test method for the first time in the day you will be suggested to run a control However once QC is done for the day no need to run another one hence F6 lt NO gt 28 BASIC Value 2 66 Value 1 88 Reagent Blk This control can be done at a regular interval defined in days Programming O Modify C Print To do so return to lt PROGRAMMING gt F1 MODIFY gt select the corresponding analysis method and validate till you obtain the following display Check frequency Value 1 in days XE Control frequency Enter the frequency in days and validate This procedure determines periodicity Control can be done daily for 31 days 29 BASIC This way daily periodically the instrument memorizes analysis results amp quality controls Status of control Use upward downward arrows to visualize 27 ALBUMIH ar control A 45 46 13937 1 B62B62719397 gt 2 870612997 Printing control results Exemple of print out analysis comprising one quality control per da SECOMAM Polyphotometer UV Vis BASIC V1 11 Date 04 06 1997 Hour 09 25 User Analysis ALBUMIN A Reagent Standard Control OK Control Le 05 06 97 AAA Normal value 38 00 54 00 8 1 O Normal value 38 00 a 54 00 g
16. PLE NUMBERING Sample numbers will be incremented automatically However you can assign another number through F2 key Example Sample 3 is to be modified Press F2 and you will see Sample Nr 1 on the screen Press Delete and replace by the number you want using number keys 45 BASIC 9 MAINTENANCE 9 1 TECHNICAL CHARACTERISTICS XE Technical characteristics PARAMETERS IN MEMORY READING MODE CALIBRATION TYPE OF CUVETTE APPROVALS LANGUAGES 94 SIMPLE ABSORBANCE END POINT WITH SERUM BLANK END POINT WITHOUT SERUM BLANK REAL KINETICS KINETIC TWO POINTS BICHROMATISM LINEAR NON LINEAR 6 STANDARDS HALOGEN LAMP HOLOGRAPHIC GRATING SILICIUM DIODE ARRAY DRY TEMPERATURE PELTIER SYSTEM AMBIENT 25 30 37 C FLOW THROUGH uCUVETTE 30 yl 10 mm OPTICAL PATH PROGRAMMABLE 500 to 1500 MANAGEMENT OF RESULTS MONTHS 110 220V SERIAL RS232C AND PARALLEL ISO 9001 AFAQ N 1995 4102 CE EMC MULTILINGUAL 47 BASIC 10 BASIC 49 Note down the analysis names you have modified SUBSTRATES ENZYMES Note down the analysis names you have modified SUBSTRATES ENZYMES Albumin Alcohol Ammonia Acid Phosphat Alk Phosphat Anti Thrombin Bicarbonate Bilirubin Amylase Calcium Chloride Cholesterol HDL Choles LDL Choles Creatinine Glucose Magnesium RBC Magnesium Phosphorus T Protein Uric Acid BASIC 11 cLossary A An
17. alysis mode B Bichromatism C Calcul mode clock set up Control frequency Cursor D Date set up Dead volume Download to a PC Win 95 Download to a PC Windows 3 x Download to a printer Elevator End point with serum blank End point without blank serum Enzyme kinetics Error messages F Front view G General set up K Keyboard Kinetics with standards L Language set up Linearity limit 30 32 29 15 23 10 15 17 19 20 21 14 31 31 31 37 13 14 34 53 M Memory initialisation Multi standard N Normal high Normal low Number keys Print out Printer set up Printer set up Program keys Pump calibration Pump set up Q Quality control set up R Result resolution S Screen Single standard T Technical characteristics Temp Trace ability set up U Unit V Volume W Wavelength 30 21 35 34 34 10 38 17 17 10 27 16 15 22 29 41 28 18 29 17
18. called a sample serum blank When there is only one reagent and such a reagent may add a parasitical color to the wavelength of the reading it is deducted from such reading this is referred to as reagent blank End point with serum blank End point with serum blank Bichromatism Conc Std abs Std Abs reagent Blank x coef Calibr End point without serum blank End point without blank serum Conc Std Abs Std Abs Serum Blank Abs Blank Abs Blank blank Coef F3 Enzyme kinetics XE 52 Enzyme kinetics Called also zero order kinetics This method calculates the evolution of a sample in Absorbance against a time period defined in a certain number of intervals BASIC computes the straight regression line which will average the means of the points BASIC allows you to select the duration of analysis reading as well as the number of intervals to be measured Also lag time between each intervals should be indicated Nx gt XY 2 slope UC Nx Y X2 X Total number of point of the kinetic curve nb of segments x segment time 1 2 3 N x N 1 2 yY total of absorbances at points 1 2 3 N A A2 A3 AN yXY Al x 1 A2 x 2 A3 x 3 AN x wX 144494 N N N 1 2N 1 6 The slope thus calculated is expressed in delta of Abs min F4 Kinetics with standards XE Kinetics with standards Called also two point kinetics or
19. cting cable is plugged To register the parameters select FILE then SAVE Windows will open a dialog box lt SAVE gt Choose in which repertory you want to save the program also give name with TRM then enter OK That file name is shown on PC s title bar To receive result file from the instrument click menu TRANSFER then RECEIPT A TEXT FILE A dialog box RECEIPT A TEXT FILE is opened Choose in which repertory you will save the data result file also under which name with TXT extension Press OK your terminal will be on reception mode e On BASIC itself press lt BASIC PC gt data will appear on PC s screen 7 6 2 Downloading to a printer F2 lt BASIC PRINTER gt Download to a printer Connect BASIC to a printer Press BASIC PRT You will obtain 26 1 2 3 xm TCP TESTI EEE 17 E 1 220 2 0 0 8 25 0 1 7 47 E 3 0 0 29 08 1997 08 20 47 E AA EI 2 oe oe oe DIDI VD J M 47 b 5 9 9 29 08 1997 08H20 A 47 E 6 9 8 1 29 08 1997 08821 BEER M 47 E 7 9 8 29 08 1997 08822 oe oe oJ 2 Column 1 Sample number characteristics B Reagent blank S Standard E Sample C Control b Blank of reagent blank Standard blank e Sample blank
20. e as soon as aspiration ceased Resume with next sample simply by introducing the tube In case of aspiration detector failure use F1 button to start aspiration MODIFICATION OF STANDARD VALUE Standard concentration value can be modified during analysis SINGLE STANDARD use F3 MODIFY XE Single standard Enter new standard value MULTI STANDARDS XE Multi standard ESTRADIO Confirm whether standard values to use are as programmed OK or remeasurement are to be done Repeat Slt H 1HH 54 4 488 Set H 2HH So 4 040 Sot 4 308 S6 4 248 Repeat OK 41 BASIC If you confirm the latter you will see 37 ESTRADIO TT Modification of each standard value can be done through the Sit p 188 54 8 08 9rresponding F button 521 H 2HH Sot 6 908 So H 4HH S56 1 BDD Standard 3 alues on display are correct validate lalue 5 ise modify using numerical keys then 3T ESTRADIO TT Reagent Blk Start with reagent blank reading This will be deducted automatically from other readings Measuring results will be displayed as ESTRADIO WBE Por Reagent Blk 4 168 ESTRADIO 27 ESTRADIO Standard Hr z The standards will be called one after another to establish standard curve to calculate sample values 42 BASIC ESTRADIO gg 35 37 m OR 51 22
21. ed function keys guide you throughout the analysis On the display Function keys correspond to an icon or a value Simply validate by a light stroke ON gt OM 15 BASIC e Cursor keys Use these keys to have access to other functions of the keyboard such as elevator mouvement BASIC test methods on the primary secondary positions next screen display right arrow for double rinse XE Cursor XE Program keys Program keys each program key can contain two test methods on the primary position and the secondary position secondary position are M1 to M47 These program keys are preassigned to give you an idea of the program capacity and calculation method the instrument is capable of You can modify any given test method at any time experiment your own parameters place it at a different key than the original and call it just by one stroke Before modifying it is recommended to print the existing test method Number keys otherwise indicated on the display numbers are to be entered by way of program keys date hour sample values sample numbers XE Number keys SUBSTRATES ENZYMES EIA M2 M3 M23 1 M24 2 1M35 3 M36 Albumin Alcohol Ammonia Acid Phosphat Alk Phosphat Ferritine IGE Qi TNS 25 A M26 5 M37 6 M38 Anti Thrombin Bicarbonate Bilirubin Estrodiol FSH MN M9 M27 M28 B 1M39 9 1 40 Calcium Chloride Cholesterol
22. en enter numbers Standard 1 1 46 Non linear E l A tube methods use this calculation mode polynomial log logit spline multi points Enter the number of standards used then the values of each Up to 6 standards can be used Humber of standards 44 J Standard 4 Value 1 68 Value 4 86 Wavelength Wavelength Wavelength to use is indicated by the reagent technical sheet You may not find the exact same wavelength among those proposed by BASIC In that case select one closest to the ideal wavelength dawelenh 2 9 XE Analysis mode 36 BASIC Analysis mode End point without I wass Serum Analysis mode Kinetics with 7 standards PT 4 End point with blank serum Enzymes kinetics F1 End point analysis mode When using this mode reading is done at the final absorbance optical density point at the end of the reaction process Zero is adjusted using reagent alone reagent blank or distilled water F2 End point with blank A number of environments in particular biological ones may be the object of spectral interferences at the wavelength selected for the dosage This is the case for colored molecules haemoglobin bilirubin These interferences which are independent of the parameter to be measured will be eliminated by measuring the sample alone or with reagent which does not trigger a coloring effect this is
23. is activated To save the parameters select lt FILES gt then lt SAVE gt Windows register selected parameters under predefined name To receive result from the instrument click on lt TRANSFER gt then lt CAPTURE TEXT menu 25 BASIC Windows opens a dialog box lt CAPTURE TEXT gt Choose in which repertory you will save the data result file also under which name either by entering the pathway or the name with lt TXT gt extension or by selecting lt FOLLOW gt icone Type lt start gt and your terminal will be on reception mode On BASIC itself press lt BASIC PC gt button you will see its data on the PC screen FOR WINDOWS 3 x Open lt TERMINAL gt program which is usually under program group lt ACCESSORIES gt To create acquisition program do the following Select lt PARAMETERS gt lt TERMINAL EMULATION gt and select lt DEC VT 100 AINSI gt option Then select lt PARAMETERS gt lt TERMINAL PARAMETERS gt then the following options Character terminal terminal column 80 CR gt CR LF Entry O Exit Continue by selecting PARAMETERS COMMUNICATION and define the page as follows transmission rate 9600 bauds Data bits 8 bits Stop bits 1 bit Parity none Flux control none Parity control L non selected Beared detection L non selected Download to a PC Windows 3 x Then choose COM number where the conne
24. orking days shipping delay not included By express agreement the following are strictly excluded from our guarantee All damages notably for staff costs loss of earnings business trouble etc Any breakdown due to an incorrect use of the equipment non adapted mains fall attempt at transformation etc or to a lack of maintenance by the user or to poor storage conditions Any breakdown due to the use of parts not supplied by SECOMAM on SECOMAM equipment Any breakdown due to the transporting of the equipment in packaging which is not its original packaging The lamps the cells and generally any item which appears in the accessories section on the price list Our customers are kindly asked to apply for our consent before returning any instrument for repair No return of materials may be accepted without the prior written consent of our Sales Management which will precise the terms of such return If the above consent is given articles shall be returned in their original packaging on a prepaid basis to the following address SECOMAM 91 avenue des Pins d Alep 30100 ALES FRANCE We reserve the right to reship all instruments received collect failing such consent Whatever method and conditions of transport are chosen for the shipment of the equipment to be repaired under guarantee in the original packaging the corresponding costs and the insurance costs will be payable by the customer Any damage connected to
25. ormal range high low check frequency previous results amp standard control will be crushed upon saving a new method Note At any case arbitrary values are labeled by small arrows on the right or the left side of the value XE Program keys 8 1 PROGRAM KEYS To program an analysis test simply return to set up procedure CONFIGURATION then PROGRAM gt PROGRAMMING F1 MODIFY gt C Modify Next you will obtain the lt ANALYSIS gt selector panel simply press T or 4 or cursor key for access to program keyboard HHHL YSIS SUESTRATES SUBSTRATES _ u H ME Primary position Secondary position 33 BASIC Select one of the program key to place the new test method ALEUMIH ADJUSTMENTS RELATED TO ANALYSIS Name the analysis method should be done first lt is recommended not to use the same name as the one found in the primary position DELete test method name and replace Choose letters and numbers with F1 to F6 keys and T or y cursor Validate at the end Aspiration volume 800 ul is the recommended pump aspiration volume However a selection of six volumes from 500 to 1500 ul are available To modify the volume press the F key corresponding to the volume to program Temperature BASIC is equiped with Peltier effect temperature control cell holder Recommended temperature to use is given by the test method technical shee
26. pe of port connection either serial RS232C or Parallel Connect IDP or KYOLINE printer using its serial printer cable to BASIC s serial RS232C port 28 BASIC Warning never connect a serial printer with a 25 pin cable to the parallel port This will do serious damage to BASIC and the instrument will emit a continuous beeping noise This type of error is not covered by the warranty Printer landusde Printer connection Exit from set up by lt ESC gt 7 6 TRACEABILITY SET UP XE ETE set up CONFIGURATION Data identification filing report etc are given in this F6 TRACE ABILITY function F1 BASIC PC this function allows you to download data patient reports to the PC see chapter 5 6 1 F2 BASIC PRT use this function to download data to a printer Two types of printed documents can be obtained e global daily report of all patients enter Nr 0 or e patient by patient report selectable example Nr 114 121 You can print previous day s result if it is not crushed erased yet by reprogramming the date see CONFIG DATE Note For two samples bearing the same number only the latest will be edited is used to clear result memory Be sure to save print results before deleting F4 YES by choosing this function BASIC will report kinetical reading points per segment F5 NO by this function BASIC will give brief kinetical report F6 ORDER is to select the prin
27. t Use F key to program the temperature Note during warm up the programmed temperature digits will blink rather fast at first slowing down afterwards It takes 15 minutes to stabilize the cell holder and cuvette to 37 C Once temperature is reached the digits will be displayed permanently Peltier effect is deactivated when programmed at XX Room temperature F1 Note on work organization it is adviced to run all test methods using the same temperature in one session to avoid warm up delay For your convenience the cell holder will remain on the last Room tem temperature used Unit Choose measuring unit according to the recommendation of the reagent technical sheet 24 BASIC XE Unit Result resolution decimals Concentration result is given by the number of decimals programmed XE Result resolution Hccurate Calculation mode Calcul mode A choice of three calculation modes are proposed F1 Factor in Kinetic mode enzymatic factor coefficient value is used instead of calibration factor Enter factor value indicated on the reagent technical sheet DELete then enter numbers Results will be calculated using this factor llalue 1 HH 35 BASIC F2 Linear In End point End point with blank and Two points kinetics analysis modes calibration is needed which means the calculation is done using standard s Enter standard s value s DELete th
28. ting order of a given patient for example Nr 114 first then Nr 121 24 BASIC ES ol O ORDER C Basic PC Basic Prt Init 7 6 1 Downloading to a PC F1 BASIC PC Connect BASIC to the PC using ref 0X6807 cable and if needed an adaptator XE Download to a PC Win 95 FOR WINDOWS 95 Click on START PROGRAM ACCESSORIES HYPERTERM windows 95 opens a window where you will find HYPERTRM EXE Open lt HYPERTRM EXE gt which will give access to other window lt NEW CONNECTION gt and DESCRIPTION OF A CONNECTION Choose the name and icone of this connection bearing in mind the type of instrument which is your source of data then OK You ll see lt DESCRIPTION gt disappear to leave space to a new windows called lt TEL NUMBER gt also lt NEW CONNECTION HYPERTERMINAL gt will appear as NAME OF INSTRUMENT HYPERTERMINAL Select the COM number where the connecting cable is plugged Validate OK When lt PROPERTY COMx gt shows up and you should feed the following informations Bits second 9600 Data bit 8 Parity none Stop bit 1 Flux control none Then validate lt OK gt Check that option lt ADD LINE MODIFICATION AT THE END OF ENTERING LINES gt in menu FILES gt lt PROPERTY gt index lt PARAMETERS gt button lt CONFIG ASCII at framed level lt ASCII RECEPTION gt
29. written permission The beneficiary must attach a copy of this document to all authorized partial or total reproductions BASIC 3 INFORMATION The SECOMAM equipment has been designed manufactured tested and inspected according to the ISO 9001 standards If the unit is not immediately installed it should be stored in a dry and clean area The storage temperature should be between 10 and 35 SECOMAM equipment is carefully inspected before it is packed As soon as you receive your equipment check the condition of the packaging and if you notice any problems notify your carrier within 48 hours Then consult the packing list and check that everything is in order Finally if you discover that something is missing or if the goods are damaged immediately notify SECOMAM IMPORTANT In order to benefit from SECOMAM s service application notes SECOMAM information technical assistance etc immediately complete the attached guarantee card and return it to the following address SECOMAM Service PRODUIT 91 Avenue des Pins d Alep 30319 ALES Cedex FRANCE Tel 33 4 66 54 35 60 Fax 33 4 66 54 35 69 E mail info secomam com Service Department 33 4 66 54 35 63 Technical support 33 1 39 35 42 12 BASIC PRECAUTIONS OF USE m Always make sure that the instrument is connected on the good voltage Between 100 240V 50 60Hz Always disconnect the mains plug before starting any work inside the instrument
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