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        TC-324C User`s Manual
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1.           ration Defects  Thermistor Considerations                       2          Operation     Automatic Mode  Manual Mode escus  Connection to External Devices        ld  InzEine Healers  mm Ote RUE 10  USING Other Devices    oerte pc tesa rin 10  Appendix                      terreas wcll                                 caste rer         enr Ps 11  Accessories and replacement parts                                                 11  CC 35 Connector pin designations                                                  13    Warranty and Service isses 13  Warranty  Service Nol8S        trn tie v i d eet 13                     Publication 5700 001 REV E    sjueunJijsu                  191103002 19 eeH  euueu                    Single Channel Heater Controller    Warner Instruments    Introduction       The TC 324C Single Channel Automatic Heater Controller from Warner  Instruments is a versatile and simple to use thermal control device   Although designed for use with Warner Series 20 chambers or in line solu   tion heaters  this instrument can be easily adapted for use with custom built  equipment according to the requirements of the user     Total automatic control of heating power is provided in automatic mode   while manual control is available in manual mode  A loop speed selector is  used to optimize the response of the system to accommodate the thermal  delay characteristics intrinsic to the environment     Features of the TC 324C include        Dual thermistor design  
2.    Automatic and manual modes     Control thermistor fault  open  detection   e Selectable loop speed optimizes system speed and stability    Selectable temperature set modes  internal external       Simultaneous monitoring of system temperature and a separate  point of interest      Optimized for use with Warner perfusion and imaging chambers    Compatible with SH 27B In line Solution Heater  etc  e Provides 22 W of heating power into a 100 load     THIS EQUIPMENT IS NOT DESIGNED NOR INTENDED       FOR USE ON HUMAN SUBJECTS    Nomenclature    Text Conventions    This manual refers to device controls at two functional levels  specific con   trols and settings of these controls To minimize the potential for confusion   we have employed several text conventions which are specified below   Since our goal is to provide clarity rather than complexity  we  welcome any feedback you may wish to provide     e Warner Instrument product numbers are presented using bold type     References to controls are specified using SMALL CAPS     References to control settings are specified using italic type     e Special comments and warnings are presented in       Any other formatting should be apparent from context     Publication 5700 001 REV E    Control Description       Front Panel    A schematic of the face panel of the TC 324C is shown below  Important  components are identified by letter  B H  J   Please refer to this diagram as    an aid in identification of described components        T
3.  remplac   par l utilisateur   veuillez nous contacter pour toutes r  parations     H  The device should be operated and stored in a temperature  range of 5 35  C  41 95  F  and humidity range of  5 95  noncondensing relative humidity     D Installation Clearances  Instrument shall have 5 cm clearance on  both sides when rack mounted and 12 cm side clearances other   wise  Instrument should not be stacked on top of heat produc   ing equipment without a minimum spacing of 9 cm     J  This device features a three prong grounded power cord  Do  not remove the ground pin or operate the device when  ungrounded     K  The two disconnecting devices for this controller are the appli   ance coupler on the back panel and the outlet plug cap  Both  disconnects should remain accessible during operation of the  device     NOTE  If this equipment is used in a manner not specified by  the manufacturer in this user manual  the protection provided  by the equipment may be impaired     IMPORTANT   CUSTOMERS OUTSIDE OF THE U S   Please be sure  to contact us before return shipping any goods  We will provide  instructions so that the shipment will not be delayed or subject to  unnecessary expense in clearing U S  Customs        Publication 5700 001 REV E    Declaration of Conformity  CE MARKING  EMC  and CB Scheme    We   Manufacturer s Name   Manufacturer s Address     Harvard Apparatus Warner Instruments  84 October Hill Road   Holliston  MA 01746   Tel   508  893 8999    We herewith delcare t
4.  the MAIN POWER is On     Loop Speed Switch  Item G    Sets the feedback loop speed for the heater sensor system when the  device is used in automatic mode  Options include fast  medium  and  slow  Optimally set to fast for most applications  but can be set to  medium or slow for systems with longer thermal delay characteristics     Output Connector  Item H    Connects the TC 324C to attached heater blocks and sensor  thermistors  When used with Series 20 chambers and platforms the  CC 28 heater cable connects here  The in line solution heaters also  connect here     Thermistor Recorder Input and Output  Items J    T2 Input and T2 Output BNC connectors are provided to send  thermistor readings  T2 signals  to a data acquisition system or chart  recorder  Outputs are calibrated to 100 mV  C  T2 Input  Used as an  optional thermistor input for those configurations where a T2   Monitor  thermistor is not included in the normal setup  such as  with an In line Heater   Sensed thermistor readings can be displayed  on the meter and sent to a recording device via the standard   T2 output BNC     Up   Down LED s  Item K     Red and yellow 18   indicate relative power applied to heater     Open Thermistor LED  Item L    Should a control thermistor  T1  fail during operation  the open ther   mistor warning LED will light indicating the error mode  When an  open thermistor is detected  the device output is automatically returned  to zero volts  This design feature prevents inadvertent ov
5. EMPERATURE SET MANUAL VOLTAGE  WARNER  e e TC 324C e INSTRUMENTS  TEMP SET  HEAT UP  EXTERNAL e     HEAT pown                Ti EN Un d  POWER              TEMP MONITOR TEMP  SET TEMP  OUTPUT VOLTS  e  e MANUAL    Located directly under the Warner logo  Supplies power to the  TC 324C     Output Switch  Item B     Supplies or removes power to the OUTPUT CONNECTOR  Item H   Selects  between Automatic Mode  Manual Mode  or center off  The associated    green LED is lit when output power is on  auto or manual mode  selected     Set Temperature Control  Internal  amp  External  Item C    Used to adjust the set point of the automatic control system  The  associated green LED is on when the controller is set to Automatic  Mode on the OUTPUT sWITCH  item B      Manual Voltage Control  item D   Used to manually set the voltage output to the heater device  The    associated green LED is on when the controller is set to Manual  Mode on the OUTPUT sWITCH  item B      Meter Selector Switch  Item E     SELECTOR SWITCH selects the parameter that the METER  Item F  will    display  Options include Set Temperature  T1  Control   T2  Monitor      and Output Voltage     Publication 5700 001 REV E         sjueunijsu  JOUIEM    191103007 19 eeH                            E    Single Channel Heater Controller    Warner Instruments    Control Description  Conto        Meter  Item F     Displays the parameter selected by the METER SELECTOR SWITCH  Item E    The METER also provides an indication that
6. Single Channel  Heater Controller    Model TC 324C  Order   64 2400       TC 324C iic    eo  E  un MERERI         RE           a uc SET    i uP     9  HEA    ee    T  THERMISTOR OUTPUT    CONTROLTEMP MONITOR TEMP  SET TEMP  OUTPUT VOLTS    MONITOR TEMPERATURE  AUTOMATIC TEMPERATURE CONTROLLER       WARNER  A INSTRUMENTS    A Harvard Apparatus Company    Publication 5700 001 REV E    WEEE RoHS Compliance Statement    EU Directives WEEE and RoHS    To Our Valued Customers     We are committed to being a good corporate citizen  As part of that commitment   we strive to maintain an environmentally conscious manufacturing operation  The  European Union  EU  has enacted two Directives  the first on product recycling   Waste Electrical and Electronic Equipment  WEEE  and the second limiting the use  of certain substances  Restriction on the use of Hazardous Substances  RoHS    Over time  these Directives will be implemented in the national laws of each EU  Member State     Once the final national regulations have been put into place  recycling will be offered  for our products which are within the scope of the WEEE Directive  Products falling  under the scope of the WEEE Directive available for sale after August 13  2005 will  be identified with a    wheelie bin    symbol     Two Categories of products covered by the WEEE Directive are currently exempt  from the RoHS Directive   Category 8  medical devices  with the exception of  implanted or infected products  and Category 9  monit
7. UAL VOLTAGE control  Item D  fully counterclockwise to zero     Series 20 Chamber Setup    The CC 28 cable assembly is used to connect Series 20 Chambers to the  TC 324C Heater Controller  The chamber end of the cable includes two  connectors that attach to the resistive heaters in the heater platform  Two  thermistors are also provided  one for feedback and one for monitoring the  bath temperature  The connector end plugs directly into the TC 324C  OUTPUT CONNECTOR  Item H      Setup       Assembly instructions    1  Assemble the Series 20 Chamber as described in the instructions  supplied with the chamber     2  Connect the outboard end of the CC 28 Cable to the Series 20  platform     a  First  attach the two blue 3 pin connectors on the cable  to the heater resistors on the platform     b  Next  insert the larger thermistor  white wires or white  band  on the cable into the small hole in the side of  the platform     HINT  To assure good thermal contact between the    thermistor and platform  place a drop of mineral or  immersion oil in the thermistor hole prior to insertion        Place the small bead thermistor into the chamber reservoir     NOTE  You will most likely need to restrain the cable and  thermistor bead as the bead must be completely submerged  for accurate temperature measurements        Fill the solution bath to the desired level     HINT  For accurate temperature control  protect the microscope  stage from drafts  The solution surface offers a large surfa
8. URE control  Item C  is disconnected  as is  the feedback system of the TC 324C  The instrument now operates as a  0 15 VDC power supply that can provide up to 1 5 A of current to a device   Use this mode with heater systems that will operate properly with a fixed  voltage and lack feedback thermistors     Connection To External Devices    In Line Heaters    The SH 27A and SF 28 In Line Solution Heaters connect directly   into the TC 324C Output connector  Item H   For these devices the  temperature of the solution is monitored by a thermistor placed at  the outflow end of the in line heater  This is the T1  CONTROL   thermistor  The T1 RECORDER OUTPUT BNC  Item J  on the TC 324C  rear panel can be used to measure the temperature of this thermistor  at a scale factor of 100 mV   C  A second thermistor  plugged into the  T2 INPUT on the instrument front panel  can be used to measure the  temperature at any other point of interest     Using Other Devices    A CC 35 Cable Assembly is required for connecting other external  devices to the TC 324C Heater Controller  See the Appendix for  CC 35 pin designations     Publication 5700 001 REV E    Appendix       sjueunJjsu  JOUIE    Specifications   Maximum Output Voltage  Maximum Output Current  Minimum Load Resistance  Manual Voltage Range  Maximum Output Power  Power Requirements  Power Fuse  5 x 20 mm     Front Panel Recorder Input Output    Rear Panel Recorder Output  Temperature Range    Internal Set Temperature  External Set Te
9. ce area  for heat exchange and minimizing air flow in the immediate    vicinity will ease your temperature control requirements        Turn the controller POWER on     Switch the METER SELECTOR switch  Item E  to Set Temp and adjust the  SET TEMPERATURE Control  Item C  to the desired temperature  Observe  setting      METER     Set the Loop SPEED switch  item G  to fast   Switch the METER SELECTOR switch  Item E  to 77  Control      Set the OUTPUT switch  Item B  to auto The OUTPUT LED will be lit to  indicate that power is being applied to the heater  Observe the METER  to assure that the temperature is approaching the set temperature   The HEAT UP and HEAT DOWN LED s indicate the direction of heating control     NOTE  If the system is set up properly  this is all that is needed    to maintain a constant temperature in the chamber        Publication 5700 001 REV E    E    sjueunijsu                  191103002                          5              5    Single Channel Heater Controller    Warner Instruments    Setup  Cont d        10  Allow time for the system to stabilize at the desired  temperature  Set the METER switch  Item E  to 72  Monitor    This will display the reading of the monitor thermistor  submerged in solution  This reading will most likely be lower  than the platform set temperature due to the thermal gradient  between the platform and the solution  The temperature at the  sample  T2 thermistor reading  can be adjusted to the desired  setting by increasing 
10. curs within this period  we will  either repair or replace the faulty component s  This warranty does  not cover instrument failure or damage caused by physical abuse or  electrical stress  inputs exceeding specified limits      In the event that instrument repairs are necessary  shipping charges  to the factory are the customer s responsibility  Return charges will  be paid by Warner Instruments  Inc     Normal business hours are 8 30 AM to 5 30 PM  EST   Monday  through Thursday and 8 30 AM to 5 00 PM on Friday  Our offices are  located at 84 October Hill Rd  Holliston  MA 01746  and we can be  reached by phone at  800  599 4203 or  800  272 2775  Our fax  number is  203  776 1278  In addition  we can be reached by e mail  at support  warneronline com or through our Web page at  http   www warneronline com     Operation and Service Notes    Please refer all questions regarding service to our Engineering  Department     A  If the instrument METER light fails to light  check the fuse at the  rear panel  located in the black POWER INPUT MODULE   If the  fuse is found to be defective replace it with a 5x20 mm  0 5     slow blow fuse  0 25 A for facilities using 220 240 V line  voltages   If the replacement fuse also fails  please call  Warner Instruments for assistance     B  Occasionally  a knob on the front panel will loosen after long  use  These are  collet  style knobs and are tightened with a  screw located under the knob cap To gain access to the adjust  ment screw  pry 
11. d the optimum feedback rate     If the temperature of the CONTROL THERMISTOR is more than 0 5  C above the  set temperature  the voltage applied to the heater element will be immedi   ately set to 0 V  removing power to the heater  The output voltage will  remain at      until the temperature of the CONTROL THERMISTOR becomes less  than 0 5  C above the set temperature  at which point power will be  gradually re applied to disallow the system temperature to fall too far below  the temperature set point  It is normal for some overshoot to occur while  the temperature is stabilizing  and these features prevent the temperature  from reaching very high levels during this time     If the control themistor becomes open circuit  it will indicate a low  temperature  This will cause the Automatic Mode to raise the temperature  indefinitely  The TC 324C has a circuit that checks for an open thermistor   and removes output power to the heater to prevent overheating An LED on  the front panel will indicate an open thermistor condition     Publication 5700 001 REV E          sjueunijsu                  19103007              JQUUeYD              E    Single Channel Heater Controller    Warner Instruments    Operation       Manual Mode    In manual mode  the MANUAL VOLTAGE control  Item D  is used to set the out   put voltage to a fixed value  The voltage setting can be viewed with the  METER when the METER SELECTOR switch  Item E  is set to Output Voltage  In  manual mode  the SET TEMPERAT
12. erheating and  subsequent damage to the equipment or biological preparations     NOTE  When an open thermistor is detected the voltage output  folds back to  0 1V as long as the LED indicator is on  Once the  fault has been corrected  the LED will turn off and the system will  resume heating immediately     NOTE  In the rare event of an open or shorted OUTPUT fault   the voltage output folds back to  0 01V to protect the user and  equipment The unit must be completely powered down  30  seconds  and restarted to clear this fault mode and resume    normal operation        Publication 5700 001 REV E    Control Description       115 VAC to 230 VAC Conversion    ATTENTION    PLEASE READ BEFORE APPLYING POWER TO YOUR                  The unit has been factory set to be used with 115 VAC     If the operating voltage needs to be changed to 230 VAC  then use the    enclosed kit  power cord  1 ea  and 0 25 A fuses  2 ea   for the conversion     Note that the unit only uses a single fuse  the second fuse is sent as spare     Power Entry Module       Fuse holder side view        Push out drawer to access                                       the Spare Fuse       Active  Fuse                                        Step 1    Step 2             Depending on AC Voltage being  used  turn VAC selector switch  to 110 VAC or 220 VAC    For 90  110  115  and 120 VAC  use the 110 position     For 220  230  240  and 260 VAC  use the 220 position        Carefully  pry open the fuse holder from the r
13. hat the following product     Equipment Description   Product Name     Standards     Heater Controller  TC 324C    IEC 61326 1 ed1 0  2005 12   FCC 47CFR Part 15 2013 Subpart B  Class B    To which this declaration relates  is in conformity with the applicable EC  Directives  harmonized standards  and other normative requirements     Safety     EMC Directives  Emission      Immunity Directive     IEC 61010 1 2010  Third Edition     FCC 47CFR Part 15 2013 Subpart B   CISPR 11 2003 Group 1  All Class B     IEC61000 3 2 2000  IEC61000 3 3 2002  IEC61000 4 2 2001  IEC61000 4 3 2002  IEC61000 4 4 2004  IEC61000 4 5 2001  IEC61000 4 6 2003   IEC 61000 4 8 1993  A1 2000   IEC61000 4 11 2004    EMC and Safety compliance were evaluated by Intertek     I  the undersigned  hereby declare the equipment specified above conforms to  the above Directive s  and Standard s      Place   Date           HARVARD    APPARATUS    WARNER  M INSTRUMENTS    Publication 5700 001 REV E    United States  October 31  2013    Signature              Beth Bauman  V P  Engineering   Operations       sjueunJijsu  JouJeM    191103002               euueu               Notes       L    NS        191104002 1                                      1          1 5     1J9uJeM    Publication 5700 001 REV E    
14. ible  or will require recalibration of  the TC 324C     Publication 5700 001 REV E    Operation       The main use of a heater control device such as the TC 324C is to maintain  a constant bath temperature with minimum deviation from a set tempera   ture This is usually achieved by using a heated chamber platform  an in line  solution heater  or a combination of both     Automatic Mode    In Automatic Mode  the TC 324C maintains the temperature of the con   nected heater thermistor system at the value set by the user  The selectable  LOOP SPEED Control  Item G  is used to adjust the speed of the feedback loop  in the heater thermistor system  which controls the rate of change of its  output voltage  Non Warner heater systems with feedback thermistors can  be used in automatic mode provided they are compatible with the  TC 324C requirements     Temperature can be set by the controls on the front panel when the device  is in  internal  temperature set mode  In  external  temperature set mode  a  voltage applied to a rear panel BNC sets the temperature at 100 mV   C     LOOP SPEED is normally set to fast to provide the shortest time between the  application of power to the heater element and the sensing of temperature  at the thermistor  For heater thermistor systems with long response times   the fast setting will cause the measured temperature to overshoot the tar   get by a large amount resulting in system oscillation  For this condition  try  medium      slow settings to fin
15. ight  side by using a small flat screwdriver   Replace fuse according to voltage being used     Item   64 0100 Model TC 324C use   For 115 VAC  0 50 Amp   5x20 mm Slow Blow  For 230 VAC  0 25 Amp   5x20 mm Slow Blow                Publication 5700 001 REV E          sjueunJijsu  JOUIE    191103002               ouueu               Single Channel Heater Controller    Warner Instruments       Control Description  Conto     Rear Panel M N    T1 Output  Item M     BNC connectors are provid        ed to send thermistor read   ings  T1 signals  to a data  acquisition system or chart  recorder  Outputs are cali   brated to 100 mV C         External Temperature Set                Input BNC for adjusting the set temperature of the TC 324C  Input  functions only when internal external select switch  Item C  is set to  external  and the output mode switch  Item B  is set to auto  Units  are 100mV  C across the temperature range of ambient to 65 C  Can  be used for remote or computer control of set temperature     Ground Terminals  Item O     Provides separate connections for chassis and circuit ground  Banana  jacks are bridged when shipped from the factory     Setup       The TC 324C can be used to drive the heater blocks for Warner Series 20  Chambers or for providing power to other devices such as our In line  Solution Heaters     NOTES     a  Please refer to the front panel schematic on page 3 for    orientation     b  Turn both the SET TEMPERATURE control  Item C  and the       MAN
16. mperature    Meter Display  Meter Readouts    Enclosure  W x H x D     15 VDC   15A   10 0 O   0 0 to 15 0 VDC   22 W into a 10 O load   95 130 or 200 260 VAC  50 60 Hz    0 5 A Slow Blow for 95 130 VAC and  0 25 A Slow Blow for 200 260 VAC   T2 thermistor  monitors    BNC reporting 100 mV  C   BNC input calibrated at 10 0      at 25  C  T1  control    BNC reporting 100             Ambient to 65  C    22  C to 65  C  Ambient to 65  C    3 5 digit LED display of   C or V    Set temperature   T1  control  temperature  T2  monitor  temperature  Output voltage  to heater     43 2 x 8 9 x 30 5 cm  17 0 x 3 5 x 12 0 in     Accessories and Replacement Parts    Model Order   Description  CC 28 64 0106  CC 35 64 0109    Publication 5700 001 REV E    Cable Assembly for Series 20 Heater Platforms  Cable Assembly with Unterminated Outboard Ends    E    191103007               euueu               Single Channel Heater Controller    Warner Instruments    Appendix  Conto        Color Designation          pe   Pa              O    T2  Monitor  po No connection              Thermistor  common N C     N C            Control  Thermistor T1 N C   Heater  ground    Monitor  Thermistor T1    The 8 pin DIN  connector viewed Heater  from solder side    Publication 5700 001 REV E    Appendix       Warranty  amp  Service    Warranty    The model TC 324C is covered by our Warranty to be free from  defects in materials and workmanship for a period of two years from  the date of shipment  If a failure oc
17. oring and control instruments   Most of our products fall into either Category 8 or 9 and are currently exempt from  the RoHS Directive  We will continue to monitor the application of the RoHS  Directive to its products and will comply with any changes as they apply       Do Not Dispose Product with Municipal Waste      Special Collection Disposal Required       Table of Contents                                                   E 2    Nomenclature                     esee e enn nna 2  Text Conventions                  essent 2    Control Description                    atur tatnen tanus           PANG                                                      eer                  rate           Output Switch  Item B                         Set Temperature Control  Item C   Manual Voltage Control  item D          Meter Selector Switch  Item E        Meter  Item                                    Bs  Loop Speed Switch  Item G                                                                          Quiput Connector  ltem Al                                Thermistor Recorder Input Output  Items J     Up   Down LED s  Item                               Open Thermistor LED  Item L                   AC Conversion        T1 Input  Item M  essasi  External Temperature Set  Item        E  Ground Terminals   ltem                  tti 6    Series 20 Chamber Setup                      sse    Assembly instructions    csset rtt anre           Operating With Other Equipment    Load                
18. the cap off with a thin bladed screwdriver or  similar tool     C  Should service be required  please contact the factory  The  problem may often be corrected by our shipping a replacement  part  Factory service  if required will be expedited to minimize  the customer inconvenience     D  Instruments are inspected immediately upon receipt and the  customer is notified if the repair is not covered by the warranty   Repairs can often be completed in 1 2 days from our receipt of  the instrument     Publication 5700 001 REV E          sjueunJijsu                  191103002               euueu                    Single Channel Heater Controller    Warner Instruments    Appendix  Conto        E  If factory service is required  please observe the following  instructions     D Package the instrument with at least 3 inches of  cushioning on all sides  Use the original shipping carton  if it is available     2  Insure the shipment for its full value     3  Include with the shipment an explanation of the  problem experienced     F  Cleaning Instructions  Wipe exterior surfaces with a  clean damp cloth     G  High Voltage  Not for clinical use on humans  To avoid   AN risk of electric shock  do not open top panel  There are no  internal user serviceable parts  please contact us for repair   Haut Voltage  Non con  u pour essaies cliniques sur  l bumain  Afin d   viter le risque de choque   lectrique  ne  pas ouvrir le panneau sup  rieur  L appareil ne contient  aucune pi  ce pouvant   tre
19. the sET TEMPERATURE setting as described in  step 6 above This is an example of static solution warming and  is the simplest form of temperature control     Temperature control is more complex in a system where  solution continuously flows across the bath  The best method for  providing stable temperature control for a flowing solution   is to pre heat the solution with an in line heater in conjunction  with platform heating     Operating With Other Equipment    The TC 324C can be used to power other Warner equipment such as the  SH 27B and SF 28 Slow Flow Solution Heaters  In addition  the controller  can be used as an independent device to power third party equipment as  long as the following considerations are met     Load Resistance    The TC 324C heater controller will work well with many other  heating devices  To obtain maximum heating power  the resistance  of the heating element should be between 10 18 Q  but any element  that works at a maximum of 15 V and 1 5 A will also work with   the TC 324C     Thermistor Considerations    The TC 324C heater controller was designed to accommodate Unical  thermistors from Thermometrics  Edison  New Jersey   This family of  thermistors can be interchanged without recalibration of the  instrument  The nominal resistance of Unical thermistors is   10 0 kQ at 25 C     Other thermistors may be used with the TC 324C if the nominal  resistance also is 10 0      at 25 C  The use of thermistors with other  characteristics may not be poss
    
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