H301-OLYMPUS-IX
Contents
1. www oko lab com bape 32. 1 35mm Petri dish 1 1x3in chamber slide 1 Lab Tek 1in x2in chambered cover glass 1 Lab Tek II 1in x2in chambered cover glass 1 60mm Petri dish www oko lab com pape H301 OLYMPUS IX SUSP User Manual March 2015 1x35 M single 1x60 M single 1xGS M single For 1 35mm Petri dish For 1 60mm Petri dish For 1 1 x3 chamber slide 1xLABTEK M single 1xLABTEK II M single For 1 Lab Tek 1 x2 chambered cover glass For 1 Lab Tek Il 1 x2 chambered cover glass Figure 2 Available sample holders 3 Available Lids The following heated glass Lids are available for H301 OLYMPUS IX SUSP H301 SLIDING LID For easy sample loading and pipetting Included in code H301 OLYMPUS IX SUSP H301 KOEHLER LID 2 Reduces chamber height to 23 mm and allows imaging under Koehler illumination H301 INJECTION LID 2 Heated glass lid with two small openings sealed with flexible plastic allowing injec tion or permanent access to the sample Compatible only with sample holder 1x35 H301 LASER INTERLOCK LID 2 Heated glass lid with safety switch Connects to laser controller and auto matically turns laser off when lid is lifted Compatible only with sample holder 1x35 OPTIONAL not included with H301 OLYMPUS IX SUSP www oko lab com Paget H301 OLYMPUS IX SUSP User Manual March 2015 3 1 Sliding lid The Sliding Lid is a heated glass lid allowing for easy sample loading and pipetting The Sl
3. H301 OLYMPUS IX SUSP User Manual March 2015 H301 OLYMPUS IX SUSP Compatible with the following XY stages Compatible with the following Okolab Controllers Olympus IX SUSP GX SVR GX SFR BX3 SSU H301 T BL PLUS IX2 SFR IX2 SVL 2 UNO COMBINED CONTROLLER H401 T DUAL www oko lab com Page 1 H301 OLYMPUS IX SUSP User Manual March 2015 Index Me Component and CNS SIONS eranen E E donee cence edesencueneueueueneucdseecausscucueshuseedeue soe 3 L Same ROTE ga ere Pee ee ane E ene E Pee tT eT eC ee ee en ere 3 2 1 Available Sample HolderSsS ssessossossossossosossosessessessesoesoesoesoesossoesoesoesoesossoesoosossoosossossoosoosoo 3 SB AVAlaDIe Lids siesta eas esaaesasscaa seas sana seas saa seas ssaa sean ucaeueas usaeneasustensasustaueassstaneas seca sear setanaarsecaesae ess 4 Li SANS UC en ee eee 5 Cy KOENE CIT eee ere er Mere rare er en ORT Ore ve ee eee ee er ee err tren 6 I MVS CUION MG acceartie eda stan sda athens onsen sana tens anv cet TE E E E E bes aeas sbesaees senses eeeceaes 6 sa Eiser INGCTIOC L1G 4 inawcadanniuna dann iune a tunaneus toneneen a 7 4 Insertion of the Sample Feedback Temperature SeENnsor ccccccccccccccccccccccccccccecccecccecccecccecceecceecs 8 5 Insertion of Sample Holder into Chamber sesesescesesececesesesescesececececesesesoececececeseseseecesesececeseseeeeo 8 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic LOCKS sessesesceces
4. coecescecescecesoeceoo 9 7 Working with 1x3 and 1x2 chamber slides Magnetic lOCKS cece ccc ce nec ccnccccecccceccceecccescccesccs 11 8 Connecting the Gas Supply and Filling the Humidity Module cece ccc ceecccceccccncccesccceecccesccceeccs 12 9 WORKING with Perfusions oricine gists arose Grote pose erste a mssies aise a misses Bee ase EEEE ease Sues e ues accumu aceeeensauuews 12 10 Connecting the Chamber with XY stage s sescesescecescecesoecesoececoecesoecesoecesoecesoeceseeceseeceseeceseeces 13 www oko lab com es H301 OLYMPUS IX SUSP User Manual March 2015 1 Components and dimensions H301 OLYMPUS IX SUSP includes the following components Chamber main body with embedded temperature sensor Sliding heated glass lid with Indium Tin Oxide ITO conductive coating and embedded temperature sensor Integrated humidity module It is not necessary to select an additional humidity module 26 Distance between focal plane and upper side of the glass lid O n Sliding Lid Sample Holder Order Separately Integrated Humidty Module Chamber top view Chamber Base Figure 1 H301 OLYMPUS IX SUSP Components and Dimensions 2 Sample Holders 2 1 Available Sample Holders The following sample holders are available NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the list
5. h 2015 Grainer Petri35x10 Ibidi p Dish35mm low BD Falcon 35x10 Willco 35x10 Corning 35x10mm MatTek P35G X 14 X Figure 12 Ring selection scheme for 35 mm dish Ibidi p dish 50mm low MatTek P50G X 30 X a BD Falcon 60x15 Grainer Petri 60x15 al Corning 60x15mm Willco60x15 Figure 13 Ring selection scheme for 60 mm dish www oko lab com page LO H301 OLYMPUS IX SUSP User Manual March 2015 7 Working with 1x3 and 1x2 chamber slides magnetic locks Magnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder Figure 14 Magnetic lock for 1 x 3 chamber slide Figure 15 Magnetic lock for 1 x 2 chamber slide To lock the slide push simultaneously the buttons indicated with 3 www oko lab com bape 1 H301 OLYMPUS IX SUSP User Manual March 2015 8 Connecting the Gas Supply and Filling the Humidity Module A single silicon tubing carries output gas from the Okolab Gas Controller to the H301 OLYMPUS IX SUSP Silicon tubing connects to a gas input brass opening located on a side of the H301 OLYMPUS IX SUSP See Figure 16 Connect the silicon tubing by gently pushing it onto brass opening H301 OLYMPUS IX SUSP includes an integrated humidity module in order to maintain a high humidity pe
6. ible with sample holder 1x35 ONLY The Injection Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 6 shows chamber dimensions with the Injection Lid www oko lab com s H301 OLYMPUS IX SUSP User Manual March 2015 H301 INJECTION LID 2 23 Distance between focal plane and upper side of the glass lid Chamber top view 5 Figure 6 H301 OLYMPUS IX SUSP H301 INJECTION LID 3 4 Laser Interlock Lid The Laser Interlock Lid is a heated glass lid with a safety switch connecting to the laser controller It automatically turns the laser off when the lid is lifted NOTE Compatible with sample holder 1x35 ONLY The Laser Interlock Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 7 shows chamber dimensions with the Laser Interlock Lid H301 LASER INTERLOCK LID 2 Vv a Chamber top view gt j Figure 7 H301 OLYMPUS IX SUSP H301 LASER INTERLOCK LID www oko lab com ew H301 OLYMPUS IX SUSP User Manual March 2015 4 Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H301 OLYMPUS IX SUSP see Figure 8 Frontal and 3D views Temperature Sensor dedicated opening Temperature Sensor dedicated opening 1 Frontal view 2 3D view Figure 8 Insertion of the temperature sensor inside the chamber 5 Insertion of Sample Holder into Chamber Sample h
7. iding Lid is screwed onto the chamber Figure 3 shows chamber dimensions with the Sliding Lid Figure 4 illustrates how to remove Sliding Lid when a differ ent Lid is necessary NOTE Sliding Lid MUST BE REMOVED when using any other Lid The Sliding Lid is fixed onto the chamber with 4 screws Screws location is indicated by letter A in Figure 4 image 2 and 3 Keep Sliding Lid fully closed to access screws labeled A in image 2 Open Sliding Lid to access screws labeled A in image 3 Sliding Lid 26 Distance between focal plane and upper side of the glass lid 0 Chamber top view D Figure 3 H301 OLYMPUS IX SUSP Sliding Lid comes as standard Screws M2x6 Sliding Lid Figure 4 Assembly of the sliding lid www oko lab com pee H301 OLYMPUS IX SUSP User Manual March 2015 3 2 Koehler Lid The Koehler Lid is a heated glass lid reducing the chamber s height to 23 mm and allowing imaging under Koehler il lumination The Koehler Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 5 shows chamber dimensions with Koehler lid H301 KOHLER LID 2 23 Distance between focal plane and upper side of the glass lid nm Chamber top view mM Figure 5 H301 OLYMPUS IX SUSP H301 KOEHLER LID 2 3 3 Injection Lid The Injection Lid is a heated glass lid with two small openings sealed with flexible plastic allowing injection or perma nent access to the sample NOTE Compat
8. olders fit into the chamber base and are held in place by magnets embedded within both chamber and hold er To introduce a sample holder with the proper orientation match the red dot on the holder to the one on the chamber base as illustrated in Figure 9 abuis w ex Insert the adapter by superimposing the red points Figure 9 Introduction of the Specimen Holder inside the Chamber Base www oko lab com paged H301 OLYMPUS IX SUSP User Manual March 2015 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder Figure 10 illustrates the available magnetic locks for 35 and 60 mm dishes Threaded magnetic posts allow adjusting holder s height Magnetic Locks for 35 and 60 mm petri dishes Included in the corresponding sample holder Figure 10 Magnetic locks for 35 and 60 mm dish NOTE Magnetic locks are included with sample holder Spacing rings with variable diameter are also included in according to the needs of the user Figure 11 shows how to position the ring inside the sample holder Ring selection guide diagrams rings for 35 and 60 mm dish Figure 12 and Figure 13 respectively Figure 11 1 Insertion of the ring 2 insertion of the 35 mm dish 3 insertion of the magnetic lock NOTE Spacing rings are included with sample holder www oko lab com sie H301 OLYMPUS IX SUSP User Manual Marc
9. rcentage inside the chamber Fill the humidity module inside the chamber through the water input brass opening located on a side of the H301 OLYMPUS IX SUSP See Figure 16 using a silicon tubing 2 mm ID and a syringe NOTE Do not exceed 0 05 l Gas Input Water Input Figure 16 Connection with gas supply 9 Working with Perfusion H301 OLYMPUS IX SUSP features 8 perfusion holes for the insertion of perfusion tubing up to 2 5 mm in outer diameter Small screws plug the perfusion holes when not in use Grub screws M3x3 Remove small screws as needed before introducing perfusion tubing Figure 17 shows location of perfusion holes Perfusion dedicated opening 1 Frontal view 2 3D view Figure 17 Perfusion www oko lab com el 2 H301 OLYMPUS IX SUSP User Manual March 2015 10 Connecting the Chamber with XY stage Follow the steps shown in the images in Figure 18 and listed below in order to correctly connect the chamber with XY stage 1 Place the chamber on the stage and tighten 4 captive screws See Figure 18 Image 1 and Image 2 Captive screws housings are indicated with letter B in Figure 18 Image 2 Use a 3mm metric Allen Wrench to tighten the four captive screws while keeping the chamber sliding lid open See Figure 18 Image 2 Captive Screws Figure 18 Connection of the Chamber with XY Stage
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H301 OLYMPUS IX