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1. SDA SDI address Description Get set the SDI bus address for the sensor Argument 1 The new SDI sensor bus address as an integer from 0 to 9 Response The current or new address This will also show an address change that was issued via the SDI bus SDO Output mode Description Get set the format of the data that is output The default mode of 0 is the same as the old data output format with final engineering units added at the end of each sequence Modes 1 and 2 are for use by the manufacturer Mode 3 shows only the engineering units of data at the end of a data sequence Mode 4 shows no engineering units data Argument 1 The mode of operation to use Response The current or new mode of operation SPR Set prompt Description Turn the command prompt of PO4 on or off Argument 1 1 turn on command prompt 2 turn off command prompt Response The current or new command prompt shows as either on or off When the prompt is turned off there is no prompt after a response When the prompt is turned on there is a PO4 prompt after a response STO Store configuration to flash Description Stores the current configuration values to a non volatile flash memory This command automatically executes when a low input power fault happens before the sensor enters a low power state or when the user exits to PicoDOS Argument 1 None Response The message written on a complete command
2. 6 5 Flush sensor flow pa NS aa r e a a eE aaa ee Ed ee Ah a ae Aa Eaa i aani AEAEE AEE Eee 32 6 6 Pull a vacuum to prime SENSOM ccceccceececeeceeeeeeeeeeeeeeeeeeeeseceeceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeees 33 6 7 Bulkhead connector maintenance ooccccccnnncnnncnnnccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnonnnn non nn nnn ranma 33 6 8 Send reagent cartridges back to manufactureT oooooocccccnccccccccccnonononnnoncnnnnnnncnnnnnnnnnnn nn nnnncnncninnnnn 33 6 9 Sensor storage piirien a mavrclantydats atts nl a a eA Sytuo Sate nae Ce ad a e aE NEN Ean gaa 34 6 9 1 SOTOMAYOR dias peine det 34 6 9 2 Long term storage a A A aa aa els ato a 35 Section 7 Cycle commands lt a AAA ey 37 7 1 COnfiQuratiom COMmUmans ss oi aaa 37 12 Operation commands iii A iia 41 TS File COMMANMS iia dd iii 43 7 4 Miscellaneous command coccccccccnnncnnnncnnnnonnnnnnnnnnnonnnno anne nana n nn nn nn RR nn aar a a Ena aae aE aa 44 Table of Contents AS OA 46 Section 8 Software reference ii da AN 53 8 1 Engineenng units OUR UIE asss isinan a epia E aaa aaa a aa aa daii eino sie vieii aae 53 8 2 Raw transmittance forMat ocociccnncccnnccnnncccnccnoncnnonnoconnnnnnn i i ae EE AEE EERE aa EE EA 53 8 3 Summary file TOMA A ita 54 8 4 Raw filef ormatimer teed a o AA eee eee ele 54 8 9 COMMUNICATION SOU A a NE aie uta 55 8 6 CONMMOUPALION CONAN tds 55 SE A 55 8 8 Modes of Operation cccccceccceeeeccceceeeaeeeaeeeaseesaecaaeceseeeeee
3. S safety flag Response The current new on time in h mm ss The hours field is from 1 to 10 characters PRI Prime Description Do the priming sequence immediately 41 Cycle commands Argument 1 1 starts the priming sequence O starts the flush process while the priming sequence occurs Response The status of the priming sequence The message on or off will show if no arguments are made The message priming shows if a priming is started RUN Run Description Start a new data sample sequence now If NOS is 0 it will automatically set to 1 Argument 1 P start a priming sequence Response The message Running if a data sample sequence is started The message Priming if a priming sequence is started SDS SDI Status Description Get the status of the SDI interface board Argument 1 none Response VER n nc enabled disabled n n the interface3 board version number c the code that shows the status of operation and either enabled or disabled to show if the board is on or off 0 8 the board is being started or there is a problem 9 the board is OK but does not have power from the SDI bus 10 there is no SDI interface board or it is not in communication 11 the board is OK and has power from the SDI bus SLP Sleep Description Put the sensor in a low power sleep state for a specified time until a specified time or indefini
4. to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses 33 Maintenance Return Policy The user can purchase refurbished cartridges from the manufacturer Send the used cartridges back within 30 days after the refurbished cartridges are received to get a credit from the manufacturer Cartridges sent back after this time may not qualify for a credit The manufacturer will only accept red reagent cartridges that have been drained and flushed Do the steps below to prepare the red cartridge to send back to the manufacturer 1 Disconnect the red cartridge from the sensor if necessary 2 Make sure the cartridge is unlocked Figure 26 Fluid coupler lock Push the stainless steel tab in The coupler is unlocked 3 Attach an approximately 15 cm long section of 1 8 ID Tygon tubing to the quick disconnect inline coupling These two parts are in the manufacturer supplied spare parts kit 4 Put the other end of the tubing in an empty beaker 5 Attach the tubing with the quick disconnect coupling to the red reagent cartridge Any fluid in the cartridge will drain into the beaker 6 Fill a syringe with DI water and fill the cartridge with the water 7 Disconnect the quick disconnect coupling body from the cartridge 8 Shake the cartridge 9 Connect the quick disconnect coupling to the tubing and the cartridge again and drain the DI water in the cartridge into a beaker 10 Do steps 6 9 two
5. Dir C CycleData Run1 w Cu CycleDatalData a AN Status PO4 Plot Raw Plot Store the data from the sensor on the host PC There are two options Enter the file path e Find the folder If this is a new folder another window lets the user make sure of the selection and makes the folder The software stores this file path to use when the next software session starts 8 10 Settings tab 8 10 1 Sample collection schedule The user makes a decision about the values for the sensor to use in the Sample Settings area of the Settings tab The user can also use the Deployment Wizard to automatically set these values Status PO4 Plot Raw Plot Settings Monitor Fi Set Date and Time Units uM v Sample Settings Current Proposed Interval 0 40 00 0 40 00 Cal Frequency 1 1 Num Samples 10 10 Low Power Idle 0 00 30 0 00 30 Prime O 1 Select how frequently the sensor does a sample The format for this is hours minutes seconds The sensor takes some time to complete a sample sequence so if the interval is very small the sensor will apparently collect data continuously To take a sample once every other day for example set the interval to 48 00 00 2 Select the number of sample sequences or the start date and time The number of samples can be set from 1 to 32767 A value of 1 or a blank will set the sensor to do sample sequences as long as it has power 3 Select how frequently the sensor will do a
6. PicoDOS start message shows at the current baud rate after several seconds 7 3 File commands CPY Copy Description Copy a file to a new filename or location on the flash drive Wildcards and are permitted Note that if a destination file already exists it will be overwritten Argument 1 The file or files to be copied Argument 2 The destination of the copied file or files Argument 3 An optional V command to verify the new file or files Response The names of the source files followed by lt CR gt lt LF gt as they are copied After the last file is copied an additional lt CR gt lt LF gt shows followed by n files s copied where n is the number of files copied The message is padded with spaces so that the number field is always eight characters wide and ends with two lt CR gt lt LF gt DEL Delete Description Erase the specified file or files from the flash drive Wildcards and are permitted Argument 1 The file or files to be erased Response n file s erased N the number of files erased The response ends with two lt CR gt lt LF gt 43 Cycle commands DIR Dir Description Get a list of the files in the directory Wildcards and are permitted The character any group of one or more alphanumeric characters The any single alphanumeric character Arg
7. SUD Start time and date Description Get set the time and date for the first or next data collection sample or for a scheduled pump prime cycle No arguments returns the date and time for the next scheduled data collection sample or none If NOS is 0 it will automatically be set to 1 Argument 1 The start date as m d y optional or P to show the date and time or none of the scheduled pump prime cycle Argument 2 The start time h m s or 0 to stop data collection Argument 3 P to apply the preceding time or date and time to the pump prime cycle start time Response The current or new mode of operation UPC UPS count Description Get set the operation when an external wake signal happens on pin 1 The default 0 to bring the sensor out of a low power mode Mode 1 starts a data collection sample sequence Mode 2 sensor to show the most recent data collected Argument 1 The wake up mode Response The new or current wake mode 0 off 1 start on wake signal 2 show GLSO on wake signal WKM External wake mode Description Get the number of UPS cycles 40 Cycle commands Argument 1 None Response The number of UPS cycles that have happened from low power faults 7 2 Operation commands FLT Fault Status Description Show any fault conditions Argument 1 none Response A four character hexidecimal value
8. calibration spike after an ambient sample Change this value to 1 so that the rest of the sequences to not do the cal spike 4 Select how long the sensor will stay at full power draw The default is 2 minutes then the sensor goes to a low power mode until the next sample sequence starts Note that if this is set to a value of less than 3 seconds the sensor may not be responsive 5 Use the prime checkbox to show the effects of a prime cycle on the deployment calculator 6 Push Apply New Settings when the selected values have been entered into the Proposed variable boxes 8 10 2 Pump controls Pump Controls S Cal Ri R2 Pump Num Pumps Run Pumps Stop 57 Software reference Use the pump control boxes to select a pump to operate Enter the number of times to operate each of the pumps that are selected in the variable boxes and push Run Pump s to start the pump sequence To see how many pump operations are left in the current sequence go to the bottom of the Monitor tab and type PMP 8 10 3 SDI controls Sensors that have an SDI 12 interface have an additional 8 contact bulkhead connector The default address is 0 The user can change this address in the host software or at the set address command through the SDI port SDI 12 Address SDI Status Set SDI Address Get SDI Status The sensor does not need to be connected to an SDI 12 bus to change the address 8 11 Status tab T
9. manufacturer on page 33 for details on cleaning the red cartridge 3 Use the syringe to fill the cartridge with DI water 4 Attach the Tygon tubing to R2 5 Turn the sensor on 6 At the Settings tab type 200 in the number of pumps area 7 Push Run Pump s 8 Fill and flush each cartridge Keep flow passages filled with DI water 9 Wrap the outlet tubing around the eye bolts 10 Keep the reagent cartridges in a refrigerator 11 Replace any worn parts 12 Lubricate the bulkhead connectors 13 Attach the protective dummy plugs and lock collars 14 Attach the protective sleeve to the sensor 15 Put the sensor in its case for safe storage Maintenance 36 Section 7 Cycle commands The user can use commands as an alternative to the host software to communicate with the Cycle sensor Refer to the discussion below for details about how to use the commands Commands are limited to 160 characters which includes the Command characters are case insensitive Characters are converted to uppercase by the sensor but are echoed as they were input e Commands start with an ASCII character 0x24 and end with an ASCII carriage return lt CR gt character 0x0d e The command designator follows the e Command designators are usually 3 or 4 characters One or more arguments follow the command designator Arguments can be separated by a space 0x20 a tab 0x09 or a comma 0x2c Ifa command does not
10. mix period The sample pump both reagent pumps and the calibration standard pump operate A known amount of phosphate is added to the sample 8 Purple Spiked sample read period No pumps operate Signal output counts decrease because there is more phosphate added to the sample This means more color develops and the transmission is lower 9 Blue Final flush period The sample pump operates As with the other flush periods the output returns to a baseline value 26 Data analysis 5 3 Blank run example Counts A clean sensor will usually have a decrease in counts as it is conditioned When the user calibrates the sensor there is a shift in ambient read counts from run to run or a slight shift in the pre and post analysis rinse baseline of 50 100 counts 000000 00 02 00 000400 00 00 00 100800 001000 0012093 00 1400 001800 00 1800 ime 5 4 Good quality calibration data Counts Data such as the seven overlaid lines below shows good data The lines show flat stable flush periods downward spike during mixing no signs of bubbles and all seven lines agree well 00 00 00 00 05 00 00 10 00 00 15 00 00 20 00 00 25 00 00 30 00 00 35 00 Time 27 Data analysis 5 5 Bad data The graph below is an example of bad data caused by bubbles 28 Section 6 Maintenance Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glass
11. r Destination Folder Access Denied 1 You ll need to provide administrator permission to copy to this folder CycleHost Date created 1 3 2012 11 01 AM E Continue Skip Cancel Y Do this for all current items 2 found The Cycle software files will unzip Go to the main program file Double click on the CycleHost exe file Sensor setup O WET C a P E Search CycleHost GOM was o dl Organize y Include in library v Sharewith gt 4 WETLabs 2 Name i 4 CycleHost fl Cycleho J RE d JRE J ib i de lib m CycleHost exe d Windows Defender 5 do Windows Mail 9 Ifa Windows security warning shows push Run to continue to install the Cycle Host software ls Open File Security Warning The publisher could not be verified Are you sure you want to run this software CH Name ram Files x86 WETLabs CycleHost CycleHost exe Publisher Unknown Publisher Type Application From C Program Files x86 WETLabs CycleHost Cycle Bin Conca 7 Always ask before opening this file gPa This file does not have a valid digital signature that verifies its y publisher You should only run software from publishers you trust How can decide what software to run 2 3 Prime the sensor Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock
12. responds to the Send Data command Example address 0 SDI recorder sends OM sensor sends 00007 lt CR gt lt LF gt SDI recorder sends OMC sensor sends 00007 lt CR gt lt LF gt Refer to the Send Data command for examples of the commands that come after either the aM or aMC Start Concurrent Measurement aC Start Concurrent Measurement and Request CRC aCC Response atttnn lt CR gt lt LF gt Purpose starts a measurement in the sensor Notes a address 0 9 ttt measurement time in seconds The sensor always sends 000 nn 07 The number of measurement values the sensor will make and return after subsequent Send Data commands If a scheduled prime sequence is pending or running the sensor will ignore the Start Measurement and Start Measurement and Request CRC commands Start Concurrent Measurement aC Has 70 characters Start Measurement aM Has 35 characters Example address 0 SDI recorder sends OC sensor sends 000007 lt CR gt lt LF gt 48 Cycle commands SDI recorder sends OCC sensor sends 000007 lt CR gt lt LF gt Send Data commands that come after either the aM or aMC commands Send Data aDO aD1 Response a lt values gt lt CR gt lt LF gt or a lt values gt lt CRC gt lt CR gt lt LF gt Purpose sends data to the SDI recorder Notes aDO after aM 35 charac
13. that shows the fault status The value 0000 shows that there are no faults Table 3 Fault conditions Bit field Fault 0x0001 Invalid configuration values at startup The default configuration values are used 0x0002 The configuration values used at startup were not saved Some values may not be in effect 0x0004 Invalid operational values at startup The default operational values are used 0x0008 The operational values used at startup were not saved Some values may not be in effect 0x0010 The stored data subdirectory did not exist at startup and could not be made 0x0020 Communications to the gas gauge controller were not made 0x0040 SYS QPBCS is not set correctly GLSO Get Recent Sample Description Get the data for the most recently completed or ended sample collection sequence Argument 1 none Response The most recently completed or ended sample collection sequence followed by two pair of lt CR gt lt LF gt characters Refer to Error Reference source not found for the data format LSS Last Shutdown State Description Get the status of the last shutdown Argument 1 none Response The message ok if the shutdown was via the XIT command or power failure if the last shutdown was from a power fault ONT Run Time Power Consumption Description Get set the total on time for the sensor This number is usually reset when a new battery is connected Argument 1 The new on time in seconds Argument 2
14. the date to deploy on the Priming Start Date and Time calendar Enter the time to deploy minus 30 minutes in the Prime Start Time variable box The sensor will begin the priming cycle 30 minutes before it is deployed Enter the total samples for the deployment in the variable box next to Number of Samples Enter the sample interval in the variable box area next to Sample Interval Keep the default of 6 in the Cal Frequency variable box This is the manufacturer s recommended frequency Push Next A summary of the configuration shows Cycle Deployment Wizard Settings Summary Autonomous mode Syne to host clock Reset sample counter Reset pover consumption Data dir to existing dir RUN6 NOT RECOMMENDED Instrument units to uM Setting reagent 1 deployment volume to 250 0 Setting reagent 2 deployment volume to 250 0 Priming to start at 16 15 00 on 11 09 10 11 1 minutes from now Sampling to start at 16 45 00 on 11 09 10 41 1 minutes from now Unlimited number of samples Sample interval 1 00 00 Push Send Settings to Cycle If the user selects a configuration that will have a negative effect on the operation of the sensor a window shows A There are non recommended settings Apply them anyway Push Yes Push Yes at the next window to make a configuration report 13 Sensor setup Configuration values will still be sent to the sensor if the user does not want a report and pushes No 31 Ent
15. 0 1 Start HH MM SS Run 89 59 Software reference 8 12 2 1 Raw plot controls Control the number of plots to show in the Raw Plot tab and erase all plots from this tab Note that the default maximum number of raw plots is 6 The manufacturer recommends a limit of 10 plots E Cycle Host s n 000 Run 89 0 577 pM at 08 05 09 09 05 12 Tine and Date Settings Status Sleeping Get Settings PDT MMYOO YY HH MM SS Time to next sample Host 10 22 09 09 49 43 Tine to Prime Cycle 10 22 09 09 49 42 Run _stop_ seep lear Changes EXT C Sop after next current sample Host Computer Offload Dir C CycleDatalRunt mj Status PO4 Plot Raw Plot Settings Monitor Files 5i 17 18 18 1 91 00 08 00 0 10 00 0 18 00 Time From Sequence Start HH MM SS Run 87 Run 83 Run 89 8 13 Monitor tab This tab is generally used for troubleshooting Use the Monitor tab to look at the serial data that goes between the host PC and the sensor Copy and save the serial data in the window to another program for evaluation BB Cycle Host 5 n 000 Run 89 0 577 pM at 08 05 09 09 05 12 Pie Tools Help COM Settings Time and Date Settings Satu o com 6 Gat Settings POT MALOS vr tSS Tena to rent sampir Host 10 22 09 10 0026 Tere to Prime Rate 19000 Aechy em Settings a A Cycle 10 22 09 100025 fun so se O Rop after neatiourert sami Most Computer
16. 3 The calibration standard concentration stored by the sensor ups u The UPS counter stored by the sensor It increments each time the power is removed from the sensor when the sensor is not in a low power mode fit ffff A four character hexadecimal code that shows fault conditions within the sensor A value of 0000 shows that no faults are detected 54 Software reference 8 5 Communication setup Cycle Host s n 000 File Tools Help COM Settings com 3 v Rate pane 5 Host Coto Bd Di Status 0 w PI Set the COM port from the host software e Select the appropriate communication port from the drop down menu or Enter the port number in the variable box Go to the Tools menu then select Options to turn off the auto connect option The manufacturer recommends that the user keep the default baud rate of 19200 The Cycle software looks for available communication ports on the host PC and makes those available to the user The port defaults to None the first time the software is used Thereafter the software will try to connect to the last port used Note There is a known issue with the serial port drivers on some PCs using the Windows 7 operating system Once opened a COM port will not be available to the Cycle software or other programs until the user closes the Cycle software q A On certain Windows 7 systems the com port that was just in use will not be available for this or other p
17. A SEA BIRD COASTAL Cycle phosphate PO sensor User manual 01 2014 Edition 3 Table of Contents SECHONA IPECTICAMONS aa 3 A sethisaesl gantleaste sess satis eases acedanontancniancssaheneebcesthcs 3 1411 Bulkhead Connectors ci ii 3 a A atMexcccetcercuadescacdstec cuca seks aces tcducangie N E A AE cctcnecsnseceun ATA E 3 13 Op ica 4 14 Analytical iss ranie Seth sat on EU IA RES 4 Section Sensor SEUD aii 5 2 1 ASSOMDIC the Sensor ie 5 2 2 Install EOE e EEE EEEE EE T 8 2 3 A TNE SONS OMe rea eek onthe Pee EEE E EE E AE E E E E EE E E EEEE 9 2 3 1 Prime sensor with VacuUM oocccccnccnnncccnnnconccononoonnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 11 2 3 2 Fill sensor filters cian ai davecvdeveravecsaacceancneseaeteeaas 11 2 4 Prepare sensor for deplOy Met ooooooococococcoocconoccnnnccnnocnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnninnninns 11 Section Dep Mec 15 IM Modes of operationes n A A A 15 3 2 Set up for deployMent coocooocincccnnccccncnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nono nro EEEE EEE nn nnnnnnnnnnernnnennnnnis 15 AS NO 16 3 2 2 SDkdeployment taa Dei ia cal da tcibaddsiaddtadntndweantadeseeee 17 3 3 Deployment procedures cccccceeeececeeceeeeeeeeeeeeeeeeeeeeeeeeseceeeeaeeeaaesaaaeeaaaeeaaeaaaeaaasgeessseesseeesees 18 A 18 Section 4 Optional in laboratory performance analysis oooooooooooncconncccnccciocccicocconoccnncnnns 21 4 1 Setup for in laboratory performa
18. Offload Dr CACydR DRA 4C Ratas POS Plot Ram Plot Settings Montor Pies oo Awake 00 8 00 0 00 0 0000 8 14 Get data files Use the Cycle software to get the data that is stored in the sensor 1 Start the Cycle software if it is not already on 2 Select the Files tab 60 4 Software reference Cycle Host File Tools Help COM Settings Time and Date Settings Status Idle com 5 Get Settings PST MM DD YY HH MM 55 Time to next sample Unknown Host 01 04 12 15 04 00 Time to Prime Unknown Clear Changes Stop after next current sample Host Computer Offload Dir C CycleData vw GJ i T pane Status PO4 Plot Raw Plot Settings Monitor Files Cycle Files D Instrument Files Cycle On Board Data Storage Dir H RUN S RUNZ 00000004 TxT 00000003 TXT 00000002 TXT a EE SUMMARY TXT E D0000001 TXT Total Space 1 047 363 584 H O DATA Refresh Root Directory Listing a Create New On Board Dir Fiter TxT Hide Offloaded Current File oO Push Refresh Root Directory Listing The files stored in the sensor will show in the Files tab Enter the file directory or folder on the PC to store the data from the sensor or create a new folder Host Computer Offload Dir C CycleData Runt w a Status PO4 Plot Raw Plot 5 C CycleData Data E dataRuni Push Offload Selected File s Dir to move the data from the sensor to the PC T
19. SDI cable to the 8 pin connector on the sensor 2 Connect the other end of the SDI cable to the SDI recorder and a 12V power source Note that the power supply must supply a minimum of 2 amps 3 Configure the SDI recorder to send aM or aC commands at the chosen frequency These will start measurements on a preset schedule The sensor will ignore the aM and aC commands if a priming sequence is schedule but not complete The priming sequence will complete before the sensor starts measurements The SDI schedules when to start measurements The sensor controls whether the measurement is spiked or normal 17 Deployment Schedule the aM or aC commands at an interval longer than 35 minutes spiked measurements take approximately 35 minutes 3 3 Deployment procedures Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses The user makes the decision about which mode of operation to use then does the steps below to deploy the sensor 1 Install new cartridges on the sensor Refer to sections Assemble the sensor on page 5 Prime sensor with vacuum on page 11 and Prepare sensor for deployment on page 11 for details 2 Connect the sensor to a 12V 15 watt power supply and PC with the manufacturer supplied cable The user needs a serial to USB adapter for the supplied cable to connect the sensor to the PC 3 Start the Cycle softwa
20. a sample after this command will do a cal sequence Allowed values are 1 to 32767 If a data sample is being collected the new value will not change it Response The current or new cal frequency followed by the number of data samples before the next cal spike The two values are separated by a space character 0x20 A value of zero for the number of data samples before the next cal spike means that the next sample will run a cal spike DCA Deployment cartridge amounts Description Get set the quantity of chemicals in the sensor at the start of a deployment Argument 1 The calibration standard in mL Argument 2 The quantity of reagent 1 in mL Argument 3 The quantity of reagent 2 in mL Argument 4 S safety flag Response The current or new cartridge quantities to three decimals in milliliters at the start of a deployment The values are separated by a space character 0x20 DSD Data subdirectory Description Get set the subdirectory used to store data Argument 1 The name of the subdirectory without the root directory e g C Only subdirectories of the root directory are allowed Subdirectory names can be no longer than 8 characters Only a z and 0 9 are permitted Response The message reset when the command is completed DSI Device specific information Description Get device specific pump volumes the optical pathlength the PO offset and the scale factor Argument 1 The ambient pump volume in pL Argument 2 The pump volume of
21. ab set the calibration pump to run 100 pump cycles While the pump is in operation pull a light vacuum approximately 1 5 10 mL of the full travel of the plunger 5 After the pump has operated for 100 cycles make sure that the reagent tubing that connects the cartridges and the inlet barbs does not have any air bubbles 6 Look at the tubing from the reagent cartridges to the manifold to check for bubbles If bubbles are present do steps 1 4 again If bubbles are small it may not be possible to remove them 7 Dosteps 1 5 with R1 and R2 8 Press Flush 6 7 Bulkhead connector maintenance Lubricate the mating surfaces of bulkhead connectors at regular intervals with pure silicone spray only Allow the contacts to dry before they are connected Make sure that the pins have no corrosion which looks green and dull Make sure that the rubber seals on the pins are not delaminated Connectors should mate smoothly and not feel gritty or too resistant Figure 25 Example of damaged connector Delamination Broken pin Corrosion The manufacturer recommends 3M Silicone Lubricant spray UPC 021200 85822 Other silicone sprays may contain hydrocarbon solvents that damage rubber DO NOT use silicone grease DO NOT use WD 40 The wrong lubricant will cause the bulkhead connector to fail prematurely and the sensor will flood 6 8 Send reagent cartridges back to manufacturer Wear Personal Protective Equipment PPE
22. ample sequence Remaining The number of samples left as set by the NOS command Dia1 2 Reserved Diagnostic fields Table 6 Phosphate units designators Command Designator Description EUFO uM Micro molar phosphate concentration EUF1 mg L Milligrams per liter of reactive phosphate EUF2 mgP L Milligrams of atoms phosphorus per liter measured in the form of reactive phosphate Table 7 Step descriptions Index Step Index Step 0 Initial flush 5 Mid flush 2 1 Ambient read 1 6 Ambient read 2 2 Mix ambient 7 Mix spiked 3 Ambient PO read 8 Spiked PO read 4 Mid flush 1 9 End flush 8 2 Raw transmittance format Table 8 Raw transmittance measurement fields Field Description mm dd yy The sample date hh mm ss The sample time 53 Software reference Table 8 Raw transmittance measurement fields continued Field Description signal The transmittance signal counts 0 4095 run The run counter It increments by one for each sample sequence step An index counter that shows the current step in the sample sequence Refer to the Step descriptions on page 53 code A legacy code number that shows which pumps are in operation counts An increment counter that shows the number of transmittance measurements left in the current step of the sample sequence vin The voltage of the sensor s power supply line to two decimal places 8 3 Summary file format The summary
23. as to prevent the collection of air bubbles Data output values may change because of adsorption or primary production of a water tank The manufacturer recommends that the user do a validation of the water in the tank 4 5 NIST check standards for in laboratory performance analysis The manufacturer uses a 5 3 UM NIST traceable check standard that is used after calibration and before servicing to check the sensor s calibration This 5 3 uM check standard is also shipped to the user The user can check the sensor s calibration and validate any lab prepared standards Contact the manufacturer to get more check standard To make an analysis of the sensor s performance make sure the sensor is primed and that the data is accurate and stable The manufacturer recommends the use of deionized DI or tap water until the sensor s data is stable The sensor can be operated overnight to make sure the data output is stable 23 Optional in laboratory performance analysis 4 6 Solutions for in laboratory performance analysis 24 Change to a new solution to analyze with the sensor The manufacturer recommends the use of deionized DI or tap water until the sensor s data is stable The sensor can be operated overnight to make sure the data output is stable Note DI and tap water can contain measurable phosphate Use ultrapure millipore or equivalent 18 MOhm water to prepare check standards and blanks 1 2 3 Disconnect the sam
24. at or smock gloves safety glasses To make an analysis of the sensor s performance make sure the sensor is primed and that the data is accurate and stable The sensor can be operated overnight to make sure that the data output is stable Make sure the sensor does not run out of solution to sample or it will pull air in A 500 mL bottle will be enough solution for approximately 10 sample cycles csf 1 This will make a little more than 500 mL of waste Make sure the waste container is large enough for this volume Do the steps below to analyze the performance of the sensor in the laboratory before doing the steps to check standards 4 1 Setup for in laboratory performance analysis 1 Make sure that the sensor is connected to the host PC and a power supply and is in standby mode 2 Make sure to have 1 L of clean water with no particles over 10um 3 If necessary start the Cycle software 4 Goto the Settings tab and push Get Settings a Cycle Host s n 104 File Tools Help Time and Date Settings Status Sleeping COM Settings PST MM DD YY HH MM 55 Time to next sample COM 5 Get Settings Rat See Host 01 05 12 08 37 53 Time to Prime ate _ Apply New Settings Cycle osposjizos 37 51 Run stop Sleep Clear Changes Host Computer Offload Dir C CycleData v a Flush Jl Prime Now Proposed Interval 1 00 00 1 00 00 Max Runs 2615 Max Runs 2615 C Stop after next current sample S
25. ault idle time it will go to low power Running The sensor is in data collection mode or in a flush mode Pumping The pumps are in operation The manufacturer recommends the user use the Run Stop and Sleep buttons in the laboratory Use the Deployment Wizard for field deployments 8 8 1 Operation options Operate the sensor or set a time for the sensor to operate with the Choose Run Option window i 7 Choose Run Option Run Now Cancel Option Action Set Start Time Date Select the start times and dates to set up and operate the sensor Run Now The sensor starts to operate Cancel The window closes 8 8 2 Stop options The user has two options to stop the sensor Stop Sampling 2 el Choose a stop option Stop and Flush StopNow Continue Options Action Stop and Flush Stops the sensor and starts a flush sequence of approximately 5 minutes Do this at the end of a sample sequence Stop Now Stops the sensor The manufacturer recommends this only if the user starts a new sample sequence soon after the sensor is stopped Continue Closes the window The sample sequence will not stop 8 8 3 Low power option Put the sensor into a low power mode from a standby mode before power is removed 56 Software reference 8 9 Data file storage Choose the folder on the host PC in which to store the data collected by the sensor Host Computer Offload
26. ber 104 The firmware version is 1 03sd SDI recorder sends Ol sensor sends 013WET LABS CYCLEPO4___104_1 03sd lt CR gt lt LF gt 47 Cycle commands Start Measurement aM Start Measurement and Request CRC aMC Response attn lt CR gt lt LF gt Purpose starts a measurement Notes a address 0 9 ttt measurement time in seconds The sensor always sends 000 n 7 The number of measurement values the sensor will make and return after subsequent Send Data commands If a scheduled prime sequence is pending or running the sensor will ignore the Start Measurement and Start Measurement and Request CRC commands The sensor needs approximately 1800 seconds to complete a measurement The SDI 12 specification allows measurement times from 000 to 999 The sensor uses a store and forward technique to work around this limitation The sensor always sends the measurement time as 000 seconds Measurements are saved in first in first out FIFO memory If the SDI recorder sends a Start Measurement command and subsequent Send Data command the sensor sends the next set of data in the FIFO If there is no stored data the sensor sends a null response a lt CR gt lt LF gt The data recorder or data processing program needs to handle these values appropriately The sensor has the same response to Start Measurement and Request CRC except that it appends a 3 byte CRC to the data when it
27. cations Section2 Sensor setup 2 1 Assemble the sensor Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses The Cycle PO sensor comes in two boxes One is a fiberboard box with the reagent cartridges The second is a ruggedized plastic case with the sensor and spare parts 1 Remove the sensor and the spare parts kit from the plastic case 2 Remove the cartridges from the fiberboard box 3 Set the sensor on its side to remove the seven screws that attach the protective sleeve to the sensor 4 Remove the seven Phillips screws on the sleeve Keep the screws Figure 1 Screws removed from protective sleeve 5 Hold the bottom of the sensor and bring it vertical to slide the protective sleeve off Figure 2 Sensor with protective sleeve removed 6 Look at the sensor One side has the intake tubing to the sample port marked with Se Sensor setup Figure 3 Intake tubing Figure 4 Reagent tubing Cartridge color Location on sensor Contents Blue S phosphate Yellow R1 ascorbic acid Red R2 sulfuric acid lt 10 7 Remove the reagent cartridges from the box and unwrap each cartridge Note that the cartridges are indexed so each one will only fit in one place on the sensor 8 Install the blue calibration cartridge first Hold it above the upper housing above the intake
28. ct a syringe to a 25 cm length of 1 8 inside diameter ID Tygon tubing 5 Pull a minimum of 10 mL of 2 cleaning solution into the syringe 29 Maintenance Figure 20 Micro 90 pulled into syringe EA i A OF AR a 6 Connect the other end of the tubing to the S barb Figure 21 Tubing connected to sensor s e 7 Inject the contents of the syringe into the tubing Figure 22 Tubing in cleaning solution 8 Disconnect the syringe and put the tubing it was connected to into the bottle of cleaning solution 9 Go to the Settings tab of the Cycle software and push Flush The sensor takes 5 minutes to fill with the cleaning solution 10 Let the solution soak in the sensor for approximately Y hour to 1 hour 30 Maintenance 6 2 Replace reagent cartridges Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses 1 Remove the blue calibration cartridge first Press the stainless steel coupler on the bottom of the cartridge to release it 2 Slide the cartridge up and off the guide pins 3 Pull the cartridge away from the housing and set the cartridge aside Figure 23 Blue cartridge removed 4 Remove the yellow and then the red cartridges 5 Install new cartridges Refer to the section on Assemble the sensor on page 5 for details on this procedure 6 3 Replace intake filter and screen Disconnect the tubi
29. eeeeeeeeseeseecseaaaesaeaacseeesseeeeseeeeeseeees 56 9 8 1 Operation OPtionsy iissccdnaccedccends bouts yee bens desde eos bese hones ode Rida 56 89 2 Stop tati 56 9 0 3 LOW POWEF Option cerceii nie e a AE EAEE E 56 8 9 Data file Storage siii a a A deve Aaa A TE Aa A id ida 57 8 10 Settings tabr e O e a A a A a er err ee rere eer RCA 57 8 10 1 Sample collection schedule oooooococococccicnonicococconoconnccnnccnnccnnncnnnonnnnnnnnnnnnnnnnnnnnnnnnnninnnininnns 57 8 10 2 PUMP CONTO S a i a AA sence beige RE Ea o E A E EEA 57 AS A O 58 8 11 Status tab a a a E ease aa p aE A a a ERE REEE EE 58 9 12 Plot ta bS ea a a a E E 58 38 121 POF plot taD a deidad 59 9 1 2 1 1 POZ plot controls serier e ae aeee a Ra eE eine beaks dane Ei S 59 8 122 Raw plot tab eein p cai 59 8 12 21 Raw PIOE CONTOIS aneen eee a eke Hate Badia es 60 OVS IMOMIMON TAD A 60 8 14 Get data ES stevie wivaeivi cinta tid atv E E E tire tern alae ian tive ra e ahd 60 Section 9 General informati0NM ooooooocinccinnccciocccoococonccconocconononnncnnncnnonnononnnnnnnnnnnn nono nn nn nn nan nnnannnnns 63 9 1 Wa Wise thae 63 9 2 SEIVICE ANA Mia A A IIA gaa mes 63 9 3 Waste electrical and electronic equipMeNt ooooooonnooococccnoccnoncnoonnno nono nono 63 Section 1 Specifications 1 1 Mechanical Height w handle 56 cm Diameter 18 cm Weight in air 6 8 kg w reagents Weight in water 0 37 kg Depth rating 200 m 1 1 1 Bulkhead co
30. er a name and location to store the report Use txt or log as the filename extension 32 Push Save 33 Push Finish then Yes to make a results report The results report records the new values and the previous values Cycle Deployment Wizard oo Awake PO4 gt WKM D PO4 gt UKM O o PO4 gt CLK 04 17 09 PO4 gt CLK 04 04 17 09 PO4 gt VOL 11 875 2 190 27802 oe PO4 gt VOL 0 00 0 00 0 00 0 00 5 0 000 0 000 0 000 0 000 PO4 gt CNT The software will put the sensor into a low power mode 34 Disconnect the sensor from the power supply 14 Section 3 Deployment 3 1 Modes of operation There are six modes of operation Both raw and engineering units for each sample are stored in the sensor s memory Cycle modes of operation Host controlled mode The sensor is connected to a host PC and is controlled and monitored by the Cycle software The user can look at the sensor s data output and other status indicators in this mode Autonomous mode The sensor operates by itself for example installed on a mooring with a battery pack to supply power Deployment the sensor is installed on a mooring that has no controller or data logger The power is supplied by a battery pack Asynchronous slave mode The sensor is connected to a master controller At certain intervals the controller pulls the most recent data from the sensor The sensor collects data on its pre determined schedule independent of t
31. es PPE includes a laboratory smock safety glasses and gloves Replace the Cycle reagent cartridges and intake filters approximately every 1000 samples and clean the optics flow path Change the battery core of the battery pack if that is the power source for the Cycle The sensor is calibrated to output a reactive phosphate concentration in user defined units of UM mg L or mgP L The sensor will operate for approximately ten 1000 sample deployments between service and re calibration by the manufacturer 1 Put the sensor on its side and use a Phillips screwdriver to remove the seven screws that attach the protective cover to the sensor Figure 18 Protective cover removed e E 2 Support the bottom of the sensor and lift into a vertical position 3 Pull the sleeve up and off the sensor Keep the sleeve and the screws 6 1 Clean sensor flow paths Clean the flow paths between each deployment with a 2 cleaning solution of Micro 90 or Liqui Nox to keep the optics clean from the products of chemical reactions which can cause a decrease in sensitivity Both solutions are available from various scientific supply companies 1 Make sure that the sensor is connected to a power supply and a PC with the Cycle software on 2 Pull the sample tubing straight off the hose barb to disconnect Figure 19 Sample tube loosened 3 Unwrap the exhaust tubing from the top of the sensor and put one end into a beaker 4 Conne
32. files have information for all the samples in each of the sensor s sub directories Pate Time Run CAPO4 VAPO4 VAS State Flush1 Amb Min Flush2 Cal Min Remaining Diagl Diag2 After the header line there are one or more lines of data collected This ends with a lt LF gt 0x0a character Refer to the Engineering units output on page 53 for details 8 4 Raw file format The raw sample text files have two header lines that come before the raw transmittance measurements then one or more lines after the raw transmittance measurements The format is shown below Ver V VV av a aa cv cc cc riv yy yy r2v rr rr ZO Z ZZZzZz CO 0 0000 cas ss ss st t ttt cc 10 53 ups u flt ffff lt 1lf gt Table 9 Raw file first header line Field Description ver V VV The firmware revision number that the file was collected under av a aa The pump volume stored by the sensor for the ambient pump volume in pL CV CC CC The pump volume stored by the sensor for the calibration pump volume in pL rlv yy yy The pump volume stored by the sensor for the R1 reagent pump volume in pL r2v rr rr The pump volume stored by the sensor for the R2 reagent pump volume in L ZO Z ZZZZ The zero offset stored by the sensor in uM CO 0 0000 The calibration pump volume offset stored by the sensor in uL Cas Ss ss The constant a value stored by the sensor st t ttt The slope threshold which is usually negative stored by the sensor cc 10 5
33. gloves safety glasses The sensor comes with de ionized DI water in all of the fluid passages The user must prime the sensor before it is turned on This will move the reagents and calibration standard into the fluid passages Table 1 Equipment needed to prime the Cycle User supplied Manufacturer supplied 2 receptacles for water 50 mL syringe Regulated power supply Test cable PC Ya outside diameter OD tubing lt 1 m in length 1 Find the in OD tubing connected to the S mark on the meter 2 Pull the tubing from the hose barb next to the S straight off the barb The barb is angled up Sensor setup Figure 9 Y shaped tubing disconnected from S Unwind the exhaust tubing from the top of the sensor Put one end of the exhaust tubing in an empty receptacle Fill another receptacle with 150 mL or more of DI water Connect the 50 mL syringe to a length of 1 4 tubing 7 Put the end of the tube in the de ionized water and fill it with water using the syringe Figure 10 Tube filled with DI water E E 8 Make a kink in the tube near the syringe so that no water drains from the tube and remove the syringe Figure 11 Syringe removed from filled tube 9 Keep the tube with a kink in it and push it onto the hose barb near the S mark Figure 12 Sensor ready to be primed 10 10 11 12 Sensor setup Make sure to prime the sensor with a vacuum Refe
34. he SDI 12 v1 3 specification Definitions e a is the sensor s SDI 12 address e lt CRC gt is the 3 character Cyclic Redundancy Check e lt CR gt is a Carriage Return character e lt LF gt is a Line Feed character Acknowledge Active a Response a lt CR gt lt LF gt Purpose confirms the SDI 12 address Example address 0 SDI recorder sends 0 sensor sends 0 lt CR gt lt LF gt Address query Response a lt CR gt lt LF gt Purpose reports the sensor s SDI 12 address Example address 0 SDI recorder sends sensor sends 0 lt CR gt lt LF gt Change address aAb Response b lt CR gt lt LF gt Purpose changes the sensor s SDI 12 address to b The address defaults to 0 Example address 0 SDI recorder sends 0A1 sensor sends 1 lt CR gt lt LF gt address now 1 Change address al Response allccccccccmmmmmmvvwv_ssssss_fffffff lt CR gt lt LF gt a sensor address ll 2 character SDI 12 version For example 13 for version 1 3 cccccccc 8 character vendor identification For example WET LABS mmmmmm 6 character sensor model For example _CYCLE vvvv 3 character sensor version For example PO4 _ssssss up to 6 character serial number field Includes leading space _fffffff up to 7 character firmware version field Includes leading space Example address 0 serial num
35. he Status tab shows the estimated volumes of chemicals in each of the sensor s cartridges Push Get Settings or Refresh to get information on the cartridge volumes BB Cyce Hest s n 104 8 12 Plot tabs Monitor the collected data in counts from the sensor in the Raw Plot tab and collected data in uM mg L or mgP L in the PO Plot tab Do a right click in the plot window to select how to look at the data Pq Status PO4 Plot Raw Plot Settings Monitor Files 1 000 Properties 1 100 Save as 1 200 Print 1 300 Zoomi gt Zoom Out gt a 1 400 Both Axes Counts 1 500 i Domain Axis i Range Axis 1 600 H e Select Domain Axis to see the time e Select Range Axis to see the phosphate concentration 58 Software reference 8 12 1 PO plot tab Look at the data as the concentration of phosphate in selected units Refer to Table 6 on page 53 in the Output formats section for more information E cycle Host s n 000 Run 1042 0 784 pM at 05 02 09 13 38 36 DER Time and Date Settings Status Ide Get Settings POT MMOD YY HH MM SS Time to next sample Tine to Prime Host 10 22 09 09 27 00 Apply New Settings Cyde 10 22 09 09 26 59 Run stop Clear Changes Stop after next current sample Host Computer Offload Dir C CycleData Runt Status POS Plot Raw Piot Settings Monitor Files PO4 356 356 PO4 uM 428100 7 00 AM Date MM ddiyy HH m
36. he controller The controller supplies power to the sensor Deployment the sensor is installed on a mooring with a system controller that is set to collect and send the collected data to a shore side database at regular intervals Other sensors on the mooring turn on for 2 minutes of every 15 The Cycle collects data once per hour Synchronous slave mode The sample rate of the sensor is synchronized with the controller The sensor collects data when signalled by the controller The controller supplies continuous power to the sensor Deployment the sensor is installed on a mooring with a system controller that is set to collect and send the collected data to a shore side database in real time SDI 12 mode The sensor operates in the synchronous slave mode through the SDI 12 port Commanded mode The sensor is connected to the controller and is under the control of the controller This mode has the most control over the sensor and also needs the most work to use 3 2 Set up for deployment Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses The sensor can be hung under a dock with a length of rope or installed as part of a larger system Operate the sensor 15 off vertical The manufacturer recommends full submersion of the sensor The sensor can operate in less water as long as the intake filters on the bottom of the sensor are submerged If they are
37. he user can save only one directory at a time but it is possible to select several files at the same time to store to the PC Monitor the data saved to the PC Look at the Current File area at the bottom of the software window 61 Software reference 62 Section 9 General information 9 1 Warranty Revised editions of this user manual are on the manufacturer s website This sensor is warranted against defects in materials and workmanship for one year from the date of purchase The warranty is void if the manufacturer finds the sensor was abused or neglected beyond the normal wear and tear of deployment 9 2 Service and repair The manufacturer recommends that sensors be sent back to the factory annually to be cleaned calibrated and for standard maintenance Do the steps below to send a sensor back to the manufacturer 1 Contact the manufacturer for a Return Merchandise Authorization RMA Note The manufacturer is not responsible for damage to the sensor during return shipment 2 Remove all anti fouling treatment from the sensor before sending it back to the manufacturer Note The manufacturer will not accept sensors that have been treated with anti fouling compounds for service or repair This includes tri butyl tin marine anti fouling paint ablative coatings etc 3 Use the sensor s original ruggedized shipping case to send it back to the manufacturer 4 Write the RMA number on the outside of the shipping ca
38. m ss The user can also look at stored data in this window Refer to Replay Select Files in the Files tab 8 12 1 1 PO plot controls Open the plot controls part of the PO Plot tab to look at PO data and whether it is in the range selected Go the to the Tools menu then select Options then Max PO4 Plot Threshold uM to enter this value Other controls the user can select Look at and clear data points in the plot area Look at out of range data Look at the number of data points plotted compared to the total data points received This is the counter on the upper left corner of the Plot tab Note If the sensor cannot calculate a phosphate concentration the output will be NAN not a number All NANs are counted the total number of data points No NANs are counted as valid data 8 12 2 Raw plot tab Look at the data in this tab as a quality assurance tool The raw data in counts is proportional to transmittance BB cycle Host s n 000 Run 89 0 577 pM at 08 05 09 09 05 12 File Tools Help COM Settings Time and Date Settings Status Ide com 6 Get Settings POT MMYODYY HH MM SS Time to next sample se Host 10 22 09 09 48 22 Time to Prime Rate 19200 Apply New Settings Cyde 10 22 0909 48 21 run Seep Clear C E top after next current sample Host Computer Offload Dir C CycleDatalunt 3 Status PO4 Plot Raw Plot Settings Monitor Files 4 1 750 a 00 12 00 0
39. more times 11 Put the empty cartridges into a new box with a minimum of 5 cm of protective material around the cartridges 12 Send all three of the empty cartridges to the manufacturer for credit on new cartridges 13 Follow all local laws and regulations to discard the waste water from the cartridges 6 9 Sensor storage Always flush out all of the reagents in the sensor Push Flush in the Settings tab to do this procedure 6 9 1 Short term storage 34 Make sure that the sensor is clean and has been flushed before it is put into storage for as long as a month 1 Clean any biofouling from the protective sleeve 2 Clean and flush the sensor Refer to the steps in Send reagent cartridges back to manufacturer on page 33 for details on cleaning the red cartridge 3 Make sure the cartridges are installed on the sensor 4 Wind the outlet tubing around the eye bolts Maintenance 6 9 2 Long term storage The waste solution from the sensor cartridges is Hazardous Waste Follow the applicable regulations to discard the solution Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses Make sure that the sensor is clean and has been flushed before it is put into storage for as long as several months 1 Clean any biofouling from the protective sleeve 2 Clean and flush the sensor Refer to the steps in Send reagent cartridges back to
40. nce analysis oooooononocicccccccccccncncnnnnncnnnnnnnnnnnnnnnn nono nn nnnnnnnnnnnnnnnnn 21 4 2 Water required for in laboratory performance analysis ooooonnnnnnnncccnncnnnnncccnnncnnnnnanrn o nn nnnnnnnnnnnn 22 4 3 In laboratory sensor stability analySiS oooonioiiiinioninnicocccn cc 22 4 4 Use of water tanks for in laboratory performance analySiS ooccccccnnnnnccccnncccnnononnnonnnnoncnnnnnnnnnn 23 4 5 NIST check standards for in laboratory performance analySiS cccccceeceeeeeeeeeeeeeeeeeeeeeeeeees 23 4 6 Solutions for in laboratory performance analySiS ooooocccccnnnnnnnocononnncnnncnnncninnnnnnnn nono nncnnnnnnnnn 24 Section 3 Data AMAL SIS 3st cei ceupdeas Goce sand re eae tesserae eee ER sien elements 25 A A cheikh tins chp Gale Gs cakes Gas al os Sal os aes aes ao cade Cod a sta chs Gani Si a ane 25 5 2 Operation SEQUENCE cococcccccccconcconcconnncnnnnnnno nono R RARA RRA RARA RR RAR RRA RRRRRRRRRRRRnRnnnnnnneninnnns 26 5 3 Blank TUNE A Ai AA 27 5 4 Good quality calibration Gale A ici db its 27 55s BAG NN 28 Section 6 Maintenance iii ia 29 6 1 Clean sensor flow pathS ooooooonoconccconccoonnnnnnnnnnnnnnn nono nnnnnnn nana n anna rana nn nn nnnnnnnnnnnnnnnnnnnnnnnnnrnnnnnn 29 6 2 Replace reagent cartridges ii A A A AAA Ad 31 6 3 Replace intake filter and SCreen ooonnoonnoonccconccoonconnnnnnnnnnnonnnnnnnnnnnnnnn nn rnnn rra nn nnnnnnnnnnnnnnnnnnnnnnnn 31 54 Clean macro fo lihgi ita A A A A A A 32
41. need an argument a lt CR gt line terminator follows the designator Non printing ASCII characters that occur before the that starts a command are ignored and not echoed More than one command can go on a single line if separated by semi colons 0x3b Commands execute until there are more than 160 characters per line or there is an error Use the backspace character 0x08 to remove characters from the end of the command The command interpreter will echo the backspace and send a space 0x20 and a backspace 0x08 character to delete the removed character from the monitor Set up the command interpreter with the SPR command The default is enabled which shows the PO4 gt command prompt when it is ready to accept commands Cycle shows the success or failure of user issued commands and end with lt CR gt lt LF gt characters Invalid commands will show Bad command or file name e Invalid parameters or arguments will show invalid argument s A command that cannot be accepted while the sensor is collecting a sample will show Not available while sample running 7 1 Configuration commands CAS Constant a star value manufacturer s scale factor Description Get the constant a value Argument 1 none Response The constant a value to two decimal places CCS Calibration Standard Concentration Setting Description Get set the concentration of the calibration standard Change
42. ng from the top of the two stainless steel filters Remove the two screws that hold the intake filter holder to the base plate Remove the filter housing from the base plate Loosen the set screw that holds the intake filter in the holder Push gently on the tubing fitting of the filter to remove the filter from filter housing Remove the plastic spacer from the bottom of the filter Remove the copper screen from the base plate Replace the copper screen with the new screen that came with the filters Install the plastic spacer onto the bottom of the new filter 10 Put the new filter into the filter holder O 0o N Oo Oe Ne 31 Maintenance Figure 24 10 um filter installation N too high correct height ae 11 Tighten gently the set screw that holds the filter in place Do not over tighten 12 Install the filter and the holder onto the base plate a Start one screw Hold the other side of the filter holder stable with a thumb or finger b Start the second screw c Make sure the screws are tightened evenly Try to keep the filter holder parallel to the base plate d Tighten to hand tight Do not tighten too much 6 4 Clean macro fouling Wash and scrape clean any macro fouling from the sensor to keep it in good condition Do not wash with a pressure washer Remove any anti fouling tape before the sensor is returned for servicing 6 5 Flush sensor flow paths 1 Rinse the inlet tubing or ge
43. nnectors Pin Function MCBH 6 MP connector Ground RX External wake Voltage in TX oO na AJ ww hd Analog out Pin Function Diagram of MCBH 8 MP Ground Bi directional SDI 12 V in Reserved Do not use Reserved Do not use Reserved Do not use Reserved Do not use o NIIAJ AJ wl ry Reserved Do not use 1 2 Electrical Input Main port 6 pin 10 5 18 0 V SDI port 8 pin Current draw 115 mA avg 2 A max 9 mA avg 11 mA max Low power current draw 30 pA Power dissipation 1 15 W avg 12 W max Communications RS232 ASCII SDI protocol Serial data rate 9600 115 200 baud 1200 baud Data storage 1 GB compact flash card type II External wake high voltage 3 5 20 0 V External wake low voltage 0 0 3 3 V Specifications 1 3 Optical Wavelength 870 nm Pathlength 5 cm Linearity gt 95 R 1 4 Analytical Detection limit three standard deviations of 18 MOhm water less than or equal to 0 075 uM less than or equal to 0 0023 mg L PO P Quantification limit ten standard deviations of 18 MOhm less than or equal to 0 025 uM water less than or equal to 0 0077 mg L PO P Range nominal 0 10 uM 0 0 3 mg L PO P A higher range of 0 40 uM 0 1 2 mg L PO4 P is possible but outside specifi
44. not the sensor cannot flush air bubbles which can result in poor data quality Prevent the reagent cartridges from freezing and use a sun shade to keep the cartridge temperatures below 35 C Make sure that the waste tubing does not have kinks in it when the sensor is deployed Operate the sensor at least 10 cm above the bottom of a body of water This allows for circulation around the intake filters Do not use the handle to deploy the sensor Make sure that the electrical cables have no tension The user may attach the sensor to a structure such as a mounting bracket Make sure to have a backup attachment for safety 15 Deployment e Use braided line rope not twisted nylon to support the cables bracket aluminum alignment pin base plate Mounting considerations A e brocket assembled as shown on the left can be bolted to a WET Labs IOP Profiler cage using 1 4 20 hardware Offset holes on the bracket allow mounting in several locations within the cage 2 The aluminum bracket is easy to drill and modify on site for custom installations Attaching es to the Bracket E P ign the Owel pin n the bracket with the drain slot on the bottom of the instrument This aligns the intakes to be clear of the bracket 2 Set the instrument down over the pin and rock it back against the saddles The top of the shell will slide underneath the restraining bar 3 Wrap the polypropylene straps around the instrument adju
45. ops and a flush sequence starts Stop Measurement aX_CLEAR Response aCLEARED lt CR gt lt LF gt Notes starts the SDI system All data in the FIFO buffers are cleared Any scheduled prime sequences are cleared If aX_CLEAR is sent while the sensor is running a measurement the measurement stops and a flush sequence is started aX_CLEAR Example address 0 a measurement is currently under way There is already one set of measurement data in the FIFO buffer SDI recorder sends 0X_CLEAR sensor sends OCLEARED lt CR gt lt LF gt the FIFO buffer is cleared The measurement stops and a flush sequence starts Prime aX_PRIME Response PRIME lt CR gt lt LF gt Notes sends PRIME Y lt CR gt lt LF gt and starts a prime sequence If a measurement is currently under way the prime sequence will not start correctly Use the Get Prime Status command to verify that a prime sequence started correctly aX_PRIME Example address 0 a measurement is not currently under way SDI recorder sends aPRIME sensor sends PRIME Y lt CR gt lt LF gt the prime sequence starts Prime aX_PRIME Response aPRIME Y lt CR gt lt LF gt or aPRIME N lt CR gt lt LF gt depends on prime status Notes this command sends the status of the prime sequence Y or N aX_PRIME Example address 0 a prime sequence is currently under
46. order Deployment 4 Power supply 5 6 socket test cable 6 8 socket SDI cable Power requirements and example setups The sensor must have a minimum of 10 5 VDC at 2 amps The decrease in voltage over 30 m of 18 gauge cable is approximately 2 2 V Use a standalone power supply if the SDI recorder cannot supply 2 amps Connect the negative terminal of a standalone power supply to the ground terminal of the recorder Do not connect the positive terminal of a standalone power supply to any terminal on the recorder Make sure to add the power requirements of any SDI capable sensor to the total current requirement Sample setup 1 Equipment Power requirement sensor Cycle PO 10 5 VDC 2 amps cable length 60 m 200 ft 4 4 VDC 200 x 2 2 cable gauge 18 power supply SDI recorder that supplies 12 VDC at 0 5 amps 14 9 VDC at 2 amps this setup requires a power supply that supplies 14 9 VDC at 2 amps Sample setup 2 Equipment Power requirement sensor Cycle PO 10 5 VDC 2 amps sensor SUNA 12 18 VDC 1 amp cable length 30 m 100 ft 3 3 VDC cable gauge 18 power supply SDI recorder that supplies 12 VDC at 0 5 amps 15 3 VDC at 3 amps this setup requires a power supply that supplies 15 3 VDC at 3 amps Note Set the Cycle PO and SUNA to different SDI addresses Change the Cycle from its default of 0 to 1 before deployment 3 2 2 SDI deployment 1 Connect the
47. p actions by any or all of the pumps in the sensor Reset the pump counter only when a new pump is installed 42 Cycle commands Argument 1 none Response Shows the counts for the ambient pump the calibration pump blue the reagent 1 pump yellow and the reagent 2 pump red separated by spaces These are the total counts over the lifetime of the pumps installed by the manufacturer TTS Time To Next Sample Start Description Get the time until the start of the next scheduled sample sequence Argument 1 none Response Shows the time in h mm ss until the next sample starts Shows a 0 if a sample is being collected or 1 if a sample has been stopped VOL Volumes Pumped Deployment Pump Counts Description Get set the volumes for all the pumps in the sensor for this deployment An optional argument is R S resets all four pump volumes to 0 00 Argument 1 Ambient volume from the pump in mL Argument 2 Calibration standard from the pump in mL Argument 3 Reagent 1 from the pump in mL Argument 4 Reagent 2 from the pump in mL Argument 5 S safety flag Response The current or new volumes in mL to three decimal places in the order above The values are separated by a space XIT EXIT Exit Description Stop the firmware application and go to PicoDOS Scheduled samples are stopped Same as SUD 0 Argument 1 none Response None from the sensor application The
48. ple inlet tube from the S barb Let the solution drain into the sample reservoir Flush the inside and the outside of the tube with clean water The manufacturer recommends 18 MOhm Shake to dry Refer to the steps in Prime the sensor on page 9 and be careful to not make more bubbles in the intake tube Degassing sample can minimize the formation of bubbles Section5 Data analysis 5 1 Get data files Use the Cycle software to get the data that is stored in the sensor 1 Start the Cycle software if it is not already on 2 Select the Files tab Cycle Host File Tools Help COM Settings Time and Date Settings Status Idle com 5 Get Settings PST MM DD YY HH MM 55 Time to next sample Unknown Host 01 04 12 15 04 00 Time to Prime Unknown Clear Changes Stop after next current sample Host Computer Offload Dir C CycleData v Lad Status PO4 Plot Raw Plot Settings Monitor Files Cycle Files Instrument Files Cycle On Board Data Storage Dir H RUNI S RUNZ 00000004 TXT On Board Disk Storage 00000003 TXT Free Space 950 304 768 00000002 TXT a SUMMARY TXT Sa ee 00000001 TXT Total Space 1 047 363 584 H E DATA Replay Selected Files Create New On Board Dir Filter TXT Hide Offloaded Current File o 3 Push Refresh Root Directory Listing The files stored in the sensor will show in the Files tab 4 Enter the file directory or folder on
49. ports Sensor setup Figure 5 Blue calibration cartridge installed 9 Set the cartridge on the guide pins and push down until it clicks into place If the cartridge does not click into place lift it off of the guide pins and push on the stainless steel tab of the fluid coupler to make sure it is unlocked Figure 6 Blue cartridge on guide pins Figure 7 Fluid coupler at the bottom of each cartridge 10 Install the yellow and then the red cartridges The cartridges are installed correctly if they cannot be lifted off of their bases Sensor setup Figure 8 Cartridges installed 2 2 Install the software Install the Cycle Host software from the CD that comes with the sensor or download it from the Software tab of the Cycle Host product page on the manufacturer s web site 1 na pe 7 8 Make a Cycle folder in C Program Files x86 on Windows Vista Windows 7 or Windows 8 or in C Program Files for earlier versions of Windows Make a CycleHost folder in the Cycle folder from the previous step Put the CD into the PC or go to the downloaded zip file Right click on the CycleHost zip folder and choose Extract All Extract the files to C Program Files x86 WETLabs CycleHost Select a Destination and Extract Files Files will be extracted to this folder C Program Files x86 WETLabs CycleHost E Show extracted files when complete If asked for Administrator permission push Continue
50. r to Prime sensor with vacuum on page 11 for details Connect the manufacturer supplied test cable to the sensor a power supply that can provide 2 amps and the host PC The user will need a serial to USB adapter cable Start the Cycle software 2 3 1 Prime sensor with vacuum 1 2 7 Attach the Luer lock to the 1 16 ID barb adapter and to the supplied syringe Attach the syringe with the adapter to the outlet of the 1 16 ID sensor effluent tubing that comes out of the top end flange Under the Settings tab set the calibration pump to run 100 pump cycles While the pump is in operation pull a light vacuum approximately 1 5 10 mL of the full travel of the plunger After the pump has operated for 100 cycles make sure that the reagent tubing that connects the cartridges and the inlet barbs does not have any air bubbles Look at the tubing from the reagent cartridges to the manifold to check for bubbles If bubbles are present do steps 1 4 again If bubbles are small it may not be possible to remove them Do steps 1 5 with R1 and R2 2 3 2 Fill sensor filters 1 Fill the filters with Dl water 1 Disconnect the 1 8 ID tubing that connects the filter to the S inlet barb 2 Connect the manufacturer supplied syringe to the 1 8 tubing Push clean water into the filters 3 Pinch the tubing and remove it from the syringe 4 Connect it to the S barb again to prevent the loss of prime 5 The filters
51. ratory performance analysis The manufacturer recommends the use of deionized DI or tap water until the sensor s data is stable The sensor can be operated overnight to make sure the data output is stable Note DI and tap water can contain measurable phosphate Use ultrapure millipore or equivalent 18 MOhm water to prepare check standards and blanks 4 3 In laboratory sensor stability analysis 1 Select the Raw Plot tab to see the data 2 Look at the data after 19 operation cycles to see if the data is stable Figure 15 Example of stabilized data Counts If the data is stable go to the next section If the data is not stable refer to Prime the sensor on page 9 and do the steps again to make sure the sensor is set up correctly and has not pulled in any air which will give data that is not accurate 22 Optional in laboratory performance analysis Figure 16 Bubbles the sample line unts Co 00000 0001100 000200 020300 000400 020500 000000 0007 001200 001200 001400 001500 001600 001700 COTeCO 001500 Figure 17 Shifting baseline Counts 1600 0017 00 0 1800 001900 4 4 Use of water tanks for in laboratory performance analysis When the sensor operates in a laboratory water tank temperature changes or a decrease in water flow can cause air bubbles to form The manufacturer recommends that the user operates a pump to circulate the water that crosses the sensor intake are
52. re and choose the applicable serial port 4 Turn on the power supply to the sensor 5 Push Get Settings to make sure that the software and the sensor have communication 6 Select the Tools menu then Deployment Wizard 7 Choose the desired mode to operate the sensor a SDI 12 mode choose the synchronous slave mode in the Deployment Wizard b All other modes connect the sensor to a battery pack or other power supply 8 Push Next 9 Complete the steps in the Deployment Wizard a Choose the prime and sample start times that give sufficient time to deploy the mooring b Push Finish c Push Yes to put the sensor into a low power mode 10 Make sure that the sensor is in a low power mode 11 Disconnect the sensor from the test cable and PC 12 Fill the filters with DI water Make sure there is no air in the sensor refer to Prepare sensor for deployment on page 11 for details 13 If possible keep the sensor in a bucket in approximately 20 cm of water while the sensor travels to the deployment site 14 Put the bucket of water with the sensor in it in the water at the deployment site This will keep air from getting into the sensor 15 Make sure that the waste tubing on the top of the sensor has no blockages or kinks 3 4 Retrieve the sensor 18 It is important to make sure that the sensor does not get air bubbles inside it when it is retrieved from a deployment Stop data collection before the sensor is pulled from
53. rograms until this program has been exited Sorry for the inconvenience 8 6 Configuration changes Change the configuration values in the sensor cols Help A Settings Time and Dat om l6 v Get Settings PDT MM C Host 05 te 19200 vw Apply New Settings os Cycle 05 Clear Changes 1 Push Get Settings to get the configuration values stored in the sensor unless the Deployment Wizard will be used right away The host will get the configuration values from the sensor 2 Push Apply New Settings to send new configuration values to the sensor The area around the buttons is yellow until the new values are sent to the sensor 3 Push Clear Settings to remove any pending changes 8 7 Set date and time Use the Deployment Wizard to set the date and the time in the sensor The user can also use the Control Panel of the host PC to do this 55 Software reference 8 8 Modes of operation Status Sleeping SS Time to next sample 0 39 50 1 10 Time to Prime 0 09 50 1 09 Run Stop Sleep Stop after next current sample a The sensor operates one of five modes Table 10 Sensor operating modes Mode Description Unknown The host PC has not yet received any information from the sensor Sleeping The sensor is in a low power mode Idle The sensor is not in operation and has not yet automatically gone to a low power mode If the sensor does not receive a command within 2 minutes the def
54. s or there was another error RMD Remove Directory Description Erase the specified sub directory if it is empty Note that an open subdirectory cannot be erased even if it is empty Use the DSD command to change the data sub directory first Argument 1 Path to the empty directory to erase Response Dir Removed directory is erased error code directory does not exist Dir not empty error directory is not empty TYPE Type Description Shows the specified files from the serial port There is no data verification or way to see the end of a file Wildcards and are permitted Argument 1 The files to show Response Directory then filename separated by a character then lt CR gt lt LF gt 7 4 Miscellaneous commands DAC Set DAC output Description Set the level of output of the Digital to Analog converter to a specified level 44 Cycle commands Argument 1 The 12 bit value shows in hexidecimal format from O fff Response The new DAC level of output Table 4 Common DAC output voltages Value Approximate output voltage 0 0 00 333 1 00 400 1 25 666 2 0 800 2 5 999 3 0 c00 3 75 ccc 4 00 fff 5 00 DEV Device Type Description Get the device type and serial number of the sensor Argument 1 none Response The device type and the serial number of the sensor separated by a space PO4 device type nnn
55. se and on the packing list 5 Use 3rd day air to ship the sensor back to the manufacturer Do not use ground shipping 6 The manufacturer will supply all replacement parts and labor and pay to send the sensor back to the user via 3rd day air shipping 9 3 Waste electrical and electronic equipment Electrical equipment that is marked with this symbol may not be disposed of in European public disposal systems In conformity with EU Directive 2002 96 EC European electrical equipment users must return old or end of life equipment to the manufacturer for disposal at no charge to the user To recycle please contact the manufacturer for instructions on how to return end of life equipment manufacturer supplied electrical accessories and auxiliary items for proper disposal 63 General information 64 Sea Bird Coastal 13431 NE 20th Street 0 Bellevue WA 98005 U S A www sales 425 401 7653 support 425 401 7654 support sea birdcoastal com www sea birdcoastal com WET Labs Inc 2012 All rights reserved Printed in USA
56. seconds or hours minutes seconds If 0 the sensor will collect one data sample and then stop Response The new or current sample interval as hh mm seconds NOS Number of samples Description Get set the number of data samples includes the current data sample to collect before the sensor stops Argument 1 The number of data samples to collect The default is 1 which sets no limit to the number of data samples Response The new or current number of data samples to collect OPD Output period Description Get set the data output interval Use with SDO command that looks like the old sensor data output format to specify how often a data sample is sent from the sensor The default is 5 This value also specifies how often the raw signal data is written to the raw data files Argument 1 The new output interval Makes the old version data output to show every nth LED cycle Response The new or current period of output RAT Serial port rate Description Get set the baud rate for the serial port Argument 1 The new rate Values are 19200 38400 and 57600 Response If argument 1 is used the response is Changing rate to arg1 Hit lt Enter gt when ready If no argument is used or after lt Enter gt is pushed the new baud rate shows Note that data samples collected at a baud rate other than 19200 may result in bad data The baud rate cannot be changed while data is being collected 39 Cycle commands
57. specified pumps for a specified number of cycles Files are not made and data is not collected Use the STP I command to stop a PMP command Argument 1 None to get the number of pump cycles left or a decimal code that specifies which pumps to operate Set a bit position to 1 to start the pump Response The number of pump cycles left Pumping Done pump operation is complete Code Amb Cal R1 R2 Code Amb Cal R1 R2 0 no no no no 8 no no no yes 1 yes no no no 9 yes no no yes 2 no yes no no 10 no yes no yes 3 yes yes no no 11 yes yes no yes 4 no no yes no 12 no no yes yes 5 no no yes no 13 yes no yes yes 6 no yes yes no 14 no yes yes yes 7 yes yes yes no 15 yes yes yes yes VIN Input Voltage Description Get the input voltage to the sensor in volts Argument 1 none Response The input voltage to the sensor to two decimal places It shows approximately 0 2 V lower than the voltage supplied to the sensor VSN Version Description Get the firmware version in the sensor Argument 1 none Response The firmware version n nn where n is a digit from 0 9 7 5 SDI commands 46 The Cycle sensor supports all basic SDI 12 commands Refer to the SDI 12 specification for details of the command protocol SDI 12 has been tested with the SDI 12 Verifier from NR Systems Inc http Awww sdi 12 verifier com with up to 1000 ft of 24 gauge cable Cycle commands For any command nat described below the sensor will respond according to t
58. st them to length and snap them together They should be very tight It may be easier to adjust the length before snapping the buckle 4 Tie a safety line from the stainess eyes at the top of the instrument to a secure structure How to thread the webbing through the buckle ull to tighten SY WET Labs 33 sewn end free end Cycle mounting bracket Replace any questionable hardware that is less expensive than the data from the sensor Make sure screws screw eyes brackets ropes straps zinc anodes etc are in good condition Replacement parts are available from the manufacturer or a marine supply store The sensor effluent exits through the outlet tubing Make sure the effluent flows freely and does not go onto the sensor or its mounting The effluent contains antimony and molybdenum and has a pH of lt 2 Make sure to wear the proper Personal Protective Equipment PPE to work near this effluent Refer to the MSDS that comes with the reagent cartridges for specific information Obey local state and federal laws to dispose of waste Contact the manufacturer for waste containment solutions 3 2 1 SDI operation 16 All sensors that have an 8 pin connector can operate on an SDI 12 network SDI 12 version 1 3 is supported Refer to the SDI 12 Version 1 3 specification at http www sdi 12 org for details Required equipment 1 Cycle sensor with both 6 and 8 pin connectors 2 PC with Cycle host software installed 3 SDI rec
59. t CR gt lt LF gt SDI recorder sends ODO sensor sends 0 11 0706 06 0809 0501 12 678 0 9 lt CR gt lt LF gt recorder sends 0D1 sensor sends 0 12 0GFS lt CR gt lt LF gt aDO after an aC command Example address 0 values from the previous measurement sample date 11 0707 sample time 06 0910 run number 0502 PO concentration 12 901 units of PO concentration 0 last sample state 9 battery voltage 11 9 SDI recorder sends OC sensor sends 00007 lt CR gt lt LF gt SDI recorder sends ODO sensor sends 0 11 0707 06 0910 0502 12 901 0 9 11 9 lt CR gt lt LF gt aDO after an aCC command Example address 0 values from the previous measurement sample date 11 0708 sample time 06 1011 run number 0503 PO concentration 12 234 units of PO concentration 0 last sample state 9 battery voltage 11 8 SDI recorder sends OCC sensor sends 00007 lt CR gt lt LF gt SDI recorder sends ODO sensor sends 0 11 0708 06 1011 0503 12 234 0 9 11 8FZH lt CR gt lt LF gt 50 Cycle commands Continuous Measurement aR01 Continuous Measurement and Request CRC aRCO Response a lt values gt lt CR gt lt LF gt or a lt values gt lt CRC gt lt CR gt lt LF gt Notes sensor sends the last measurement data stored in the FIFO The format is the same as aDO after an aC or aCC command If
60. t a new length to connect to the S inlet 2 Fill a clean beaker with approximately 100 mL of clean water and put the other end of the tubing in the beaker 3 Go to the Settings tab of the Cycle software and push Flush a Pull the inlet tubing out of the beaker of water Let approximately 2 cm of air into the tubing b Put the inlet tubing back in the beaker Let approximately 2 cm of water into the tubing c Do the two steps above until the inlet tubing is filled with 2 cm sections of air and water 4 Put the end of the inlet tubing in the beaker again 5 Attach a syringe with a Luer lock to a 1 16 hose barb and then the outflow tube on the sensor 6 Pull the plunger to the 15 mL mark to fill the syringe Push Flush if necessary 7 Remove the syringe and put the inlet tubing in the waste beaker 8 Do the flush two more times to make sure the sensor has been flushed three times The sensor is now clean 9 Disconnect the syringe and tubing and the inlet tubing from the S port 32 Maintenance 6 6 Pull a vacuum to prime sensor Pull a vacuum to remove air bubbles that are in the sensor Do nat pull the syringe too far This makes too much vacuum and causes more bubbles 1 Attach the Luer lock to the 1 16 ID barb adapter and to the supplied syringe 2 Attach the syringe with the adapter to the outlet of the 1 16 ID sensor effluent tubing that comes out of the top end flange 3 Under the Settings t
61. tatus PO4 Plot Raw Plot ins Monitor Files Set Date and Time Units uM v Sample Settings Deployment Calculator Estimates Current Proposed Current Cal Frequency 6 6 Days 109 0 Days 109 0 Num Samples Cal Used mL Cal Used mL Low Power Idle 0 02 00 0 02 00 Prime al R1 Used mL R1 Used mL gt R2 Used mL R2 Used mL Total Effluent L Total Effluent L Pump Controls Charge Used 4h Charge Used Ah Cal R1 R2 Pump ala al a Limiting Factor Limiting Factor Num Pumps Run Pumpls Stop SDI 12 Address 0 SDI Status SDI ok Set SDI Address Get SDI Status Current File f Set the Interval to 15 minutes 00 15 00 or as necessary for an overnight test Set the Cal Frequency to 1 Set the Num Samples to approximately 20 for an overnight test Check the Deployment Calculator to make sure there is enough reagent in the cartridges for an overnight test o O O 21 Optional in laboratory performance analysis 9 10 11 12 13 Push Apply New Settings The yellow highlights go away Go to the Files tab Make a folder for stabilization or laboratory sample collection for example Stable or Lab1 Note that the file name is limited to 8 characters Push Run Push Set Data and Time to select when the sensor starts to prime and run It takes approximately 12 5 minutes to prime the sensor 4 2 Water required for in labo
62. tely Use the serial port or the external wake signal to put the sensor in a ready state There are some characters that are baud rate dependent and cannot be used to wake the sensor The character that is used to wake the sensor is not buffered for input The manufacturer recommends that the user does an RS232 break of at least 500 ms to wake the sensor Argument 1 The number of seconds to be in a low power state No argument stay in low power until the next data sample time occurs Response The message Sleeping lt CR gt lt LF gt shows When argument 1 is used or if the sensor is going to collect a sample the message for n seconds lt CR gt lt LF gt shows where n is the smaller of the value of the commanded interval or the time to the next data sample STP STOP Stop Description Cancel a sample sequence and flush the sensor or stop immediately If the flush occurs NOS goes down as though a whole sample was completed If a sample is not being collected the SUD and SUD P move to 0 and the NOS value does not change Argument 1 no argument the flush sequence occurs the sample sequence stops The NOS does not change as though the sample sequence did not occur Response Stopped shows if there is no sample sequence Flushing shows if the user made the command without the l argument Stopping shows if the user made the command with the l argument TPC Total Pump Counts Description Get the total number of pum
63. ters are allowed For the sensor the response is 6 the first 6 values in the SDI data format table There is not space in the 35 character field for 7 values If a subsequent aD1 command is sent the sensor will send the seventh value battery voltage aDO after aC 70 characters are allowed For the sensor the response is 7 values There is space in the 70 character field for 7 values The recorder does not need to send an aD1 command because all 7 values are sent after the aDO command aDO and aD1 after an aM command Assume the sensor is set to address 0 Example 1 address 0 values from the previous measurement sample date 11 0705 sample time 06 0708 run number 0500 PO concentration 12 345 units of PO concentration 0 last sample state 9 battery voltage 12 1 SDI recorder sends OM sensor sends 00007 lt CR gt lt LF gt SDI recorder sends ODO sensor sends 0 11 0705 06 0708 0500 12 345 0 9 lt CR gt lt LF gt recorder sends 0D1 sensor sends 0 12 1 lt CR gt lt LF gt aDO and aD1 after an aMC command Example 2 address 0 values from the previous measurement sample date 11 0706 sample time 06 0809 run number 0501 PO concentration 12 678 units of PO concentration 0 49 Cycle commands last sample state 9 battery voltage 12 0 SDI recorder sends OMC sensor sends 00007 l
64. the water and before power is supplied again If data collection is not stopped before power is supplied to the sensor again it can start operation and pull air in Also do not let the reagent cartridges become empty or the pumps can make air bubbles Deployment The manufacturer recommends that the user retrieve the sensor in a bucket that has approximately 20 cm of water in it so that the sensor stays submerged for travel 1 For the SDI mode of operation stop the sample sequence on the SDI recorder This will stop the SDI but not the sensor 2 Turn the power off to the sensor and then back on to stop any active samples The sensor will store the last sample even when the power is turned off 3 For SDI mode of operation Send an aR command The sensor will send the data values from the previous sample Disconnect the cable from the sensor Remove the sensor from the mooring Connect the sensor to the host PC and power supply with the test cable Turn on the sensor Push Refresh Directory Listing under the Files tab and offload the summary txt file and any other desired files from the current data sub directory E 19 Deployment 20 Section 4 Optional in laboratory performance analysis The waste solution from the sensor cartridges is Hazardous Waste Follow the applicable regulations to discard the solution Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab co
65. the PC to store the data from the sensor or create a new folder Host Computer Offload Dir C CycleData Runt v a C CycleData Data E CA Status PO4 Plot Raw Plot 5 Push Offload Selected File s Dir to move the data from the sensor to the PC The user can save only one directory at a time but it is possible to select several files at the same time to store to the PC 6 Monitor the data saved to the PC Look at the Current File area at the bottom of the software window 25 Data analysis 5 2 Operation sequence Counts This section describes how the sensor calculates phosphate and how to interpret the quality of the data 1 2 emna ifs coreo Si ij TETI coe crew 4 5 6 8 3 7 Table 2 Cycle output periods 1 Blue Pre analysis flush period The sample pump operates Referred to as the baseline 2 Red Ambient read period Used for 100 transmittance without any absorption from phosphate reaction No pumps operate 3 Green Sample mix period The sample pump and both reagent pumps operate 4 Purple Sample read period No pumps operate The reaction curve color develops Counts decrease until complete The white circle and number show the signal used as the sample transmission 5 Blue Post analysis flush period The sample pump operates Output counts spike then increase to approximately the baseline value 6 Red Spike ambient output period No pumps operate 7 Green Spike
66. the calibration standard in uL Argument 3 The pump volume of reagent 1 in uL Argument 4 The pump volume of reagent 2 in pL Argument 5 The PO offset volume in uM Argument 6 The calibration offset in uL 38 Cycle commands Argument 7 The cell pathlength in cm Response The current setting The arguments in this table show in order from argument 1 to argument 7 separated by space characters 0x20 The pump volumes and pathlength show to two decimal places The offsets show to four decimal places EUF Engineering units format Description Get set the units to use for engineering units output Argument 1 The units to use 0 microMolar uM 1 milligrams of phosphate per liter mg L 2 mg of atoms of phosphorus per liter measured in the form of reactive phosphate mgP L Response The new or current values as UM mg L or mgP L IDT Idle timeout Description Get set the communication idle time in seconds If no communication is received within this time period while a data sample is not running the sensor goes back to a low power sleep state Argument 1 The new communication idle timeout in seconds The range is 5 to 4924 Response The new or current idle timeout in seconds INT Sampling interval Description Get set the time period between data samples referenced from one start time to the next Argument 1 The interval in seconds or minutes
67. there is no data in the FIFO the sensor sends the last data record sent the Continuous Measurement command does not start a measurement It sends data from a previous measurement aRO Example 1 address 0 values from the previous measurement sample date 11 0710 sample time 06 1213 run number 0505 PO concentration 12 890 units of PO concentration 0 last sample state 9 battery voltage 11 6 SDI recorder sends ORO sensor sends 0 11 0710 06 1213 0505 12 890 0 9 11 6 lt CR gt lt LF gt aRCO Example 2 address 0 values from the previous measurement sample date 11 0712 sample time 06 1415 run number 0507 PO concentration 12 456 units of PO concentration 0 last sample state 9 battery voltage 11 4 SDI recorder sends ORO sensor sends 0 11 0712 06 1415 0507 12 456 0 9 11 4 lt CR gt lt LF gt Stop Measurement aX_STOP Response aSTOPPED lt CR gt lt LF gt Notes stops the current measurement then turns the pumps on to flush the sensor The sensor flush takes approximately 5 minutes If aX_STOP is sent when a measurement is not underway the sensor does nothing and no flush sequence is started 51 Cycle commands aX_STOP Example address 0 a measurement is currently under way SDI recorder sends OX_STOP sensor sends OSTOPPED lt CR gt lt LF gt the measurement st
68. this value only if the manufacturer gives instruction to do so Argument 1 The new calibration standard concentration in uM Response The current new calibration standard concentration in uM to two decimal places CLK or DAT Get set the sensor s internal clock Description Get set the sensor s internal clock Argument 1 The new time in hh mm ss 24 hour clock or the new date mm dd yy if both the date and time are set 37 Cycle commands Argument 2 The new time in hh mm ss 24 hour clock if both the date and time are being set Response The current or new date and time in mm dd yy hh mm ss format The hours are in a 24 hour format The date and time are separated by a tab character 0x09 not by a space CNT Sample counter Description Get set the current or most recent sample number This is the number used in the raw file naming format When the sample counter is set the next sample run is the newly set count plus 1 Set this value to 0 at the start of each deployment Argument 1 New count value for the number of data samples completed Must be followed by a S switch to change the count Set to one less than the number of the next sample Response The current or new sample counter CSF Cal spike frequency Description Get set the frequency that a cal spike is done The default is 6 Argument 1 The new frequency The next dat
69. three characters where n is a number fro 0 9 H HELP Help Description Show the available commands Argument 1 none Response See table below CAS const a CCS calibration concentration CLK get set date and time CNT sample counter CPY source destination CSF calibration frequency DAC set dac output DAT get set date time DCA get set cartridge volumes DCS default config DEL file DEV get device type DIR dir D for data dir DSI device config DSD data subdir EUF get set units format FLT get fault status GET get file s GLSO get last output IDT idle timeout INT sample interval LSS last shutdown NOS number of samples SONT show on time OPD output period PRI priming state PMP run pumps RAT get set baud rate REN oldname newname RMD remove empty dir 45 Cycle commands RUN run sample SDA SDI bus address SDO output format SDS SDI status SLP low power sleep SPR prompt on off STO store config info STP stop sample SUD start time date TPC get total pump counts TTS time til start TYPE dump file s UPC get ups count VIN get input voltage VOL get volumes pumped VSN show version info WKM _ get set wake mode XIT exit to PicoDOS PMP Run Pump s Description Operate the
70. ument 1 The directory to list or D for the current directory Argument 2 The optional filename pattern to list For example txt Response A list of the directory with the specified filters If a file is being collected it will list on the last line separated by a space from the preceding Active file GET Get File Description Get the specified file or files Wildcards and are permitted The file move is stopped with an RS232 break signal of 500 ms or longer This is followed by the checksum for the characters that were sent before the sensor got the break signal Argument 1 The files to be moved Response All of the specified files will be moved A header line comes before each file The header line has the filename the number of the file being moved from 1 to n the total number of files moved the file size in bytes and the number of bytes that need to be moved This information is separated by a space The whole file is moved and is followed by the CRC 32 checksum of eight hexidecimal characters Data being collected will stop while the file is moved If GET is used the data output stream will have pauses for the sensor s internal requirements REN Rename Description Change the name of a specified file on the flash drive Argument 1 The path and the file to change Argument 2 The new path and the file name Response lt CR gt lt LF gt Not found error the file does not exist Rename error the file exist
71. way SDI recorder sends 0X_PRIME sensor sends OPRIME Y lt CR gt lt LF gt the prime sequence starts 52 Section 8 Software reference 8 1 Engineering units output Table 5 Data output format Field Description mm dd yy Date hh mm ss Time Run Run counter It increments by one for each sample sequence CAP04 The phosphate value calculated by the sensor then by a space then the unit designator default uM Collected data shows to 3 decimal places for uM and 4 for mg L and mgP L The data value may also be nan inf or inf then the designator VAP04 The variable a based phosphate calculated by the sensor then a space and the unit designator The data format is the same as CAP04 VAS The variable a value to 3 decimal places Used to calculate VAP04 State The last step in the sample sequence Refer to Table 7 Flush1 The mean of the last 10 transmittance signal counts in the ambient flush State 1 part of a sample sequence Amb Min The mean of the 6 transmittance signal counts that triggered the knee slope threshold reaction State 3 part of a sample sequence Flush2 The mean of the last 10 transmittance signal counts in the ambient flush State 6 part of a sample sequence Cal Min The mean of the 6 transmittance signal counts that triggered the knee slope threshold reaction State 8 part of a s
72. will drip some water after this step It is not possible to remove all the air bubbles Try to remove as many as possible 2 4 Prepare sensor for deployment PON Use the host software to make sure that the sensor is in a low power sleep mode Disconnect the test cable from the sensor the power supply and the PC Disconnect the filling tube from the S inlet of the sensor Connect the Y tube to the S inlet again Figure 13 Y tube connected to the S inlet 5 Wind the exhaust tubing at the top of the sensor 11 Sensor setup 6 Make sure to align the indentations of the protective sleeve with the eye bolts then slide the protective sleeve over the sensor Figure 14 Protective sleeve aligned with eye bolts 10 11 12 13 14 15 16 17 12 Put the sensor on its side hold the eye bolts and align the screw holes for the sensor and the protective sleeve Note that the protective sleeve is longer than the sensor The screws cannot be installed when the sensor is vertical Install the seven Phillips screws again Make sure that the sensor is connected to the host PC and a power supply and is in standby mode Put the sensor in approximately 20 cm of water If necessary start the Cycle software Go to the Tools menu Select the Deployment Wizard Fie Tools Help e Deployment Wizard Ctrl D Get Settings Ctr G Options Ctro Restore Instrument Settings The Cycle Deplo
73. yment Wizard window will appear Cycle Deployment Wizard Choose a mode of operation for this deployment Autonomous Standalone with no external controller Synchronous Slave Sampling initiated by external controller Asynchronous Slave Cycle sampling on a preset schedule O Host Continuous control via a host computer L Back Next cancel Select Autonomous Standalone with no external controller Push Next Enter the name of a directory in which to store the collected data 18 19 20 21 22 23 24 25 26 27 28 29 30 Sensor setup Note that the name can have only 8 characters Push Next The Priming and Sampling Start date and Time window appears Cycle Deployment Wizard Priming Start Date and Time Sampling Start Date and Time January __ Y January 2012 Sun Mon Tue Wed Thu i Sun Mon Tue Wed Thu Fri Sat Ea 011 Bis 7 ARANA 12 e 9 10 14 42 43 14 17 18 19 03 17 18 19 2 21 24 25 26 04 5 A 2 31 05 31 Prime Start Time 09 30 Sample Start Time 10 00 Skip Prime Cycle Number of Samples 2000 Cal Frequency 6 Sample Interval 1 00 00 Back JE Jd Cancel Set up the sensor to do a prime cycle Either use the Settings tab or the Deployment Wizard before the sensor operates Select the date to deploy on the Sampling Start Date and Time calendar Enter the time to deploy at the Sample Start Time variable box Select

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