Model 22 Syringe Pump Series User`s Manual
Contents
1. E N Rear panel showing OFF ON switch and voltage selector set for 115V Publication 5385 003 REV A p Harvard Apparatus Syringe Pump Model 22 Loading Syringes The Model 22 70 2222 will hold one or two syringes from 140 ml capacity to 0 5 ul To load syringes release the black pusher block by depressing the bronze button and sliding the block to the left Lay the syringe s on the black syringe holder where they are held in place by the spring loaded fingers Make sure that the flange on the barrel of the syringe touches the syringe holder Advance the pusher block until it touches the head of the syringe At this point the pusher can be advanced manually to clear any air bubbles from the syringe and lines To use small diameter syringes it will be necessary to unscrew the black knob that retains the syringe holder fingers remove the fingers invert them and replace Model 55 2275 has anti siphon protection Both the puser block and syringe holder have adjustable retainers to secure both the plunger and flange of the barrel 22 Pumps with Infusion and Withdrawal Models 55 2226 55 2219 and 55 2316 are infusion and withdrawal versions of the 22 Pump A reversing switch is provided This switch plugs into the 25 pin RS 232 connector at the back of the pump and reverses the direction of the motor These pumps are also provided with adjustable hardware on both the pusher and the syringe holder Adjust these retain2. e or Vn 200 EM RESPONSE response string if syntax error 210 REM 220 REM 230 REM 240 S OOR if out of range if prompt only i RESPONSE 250 IF LOC 1 gt 0 THEN S S INPUT LOC 1 1 260 P RIGHT S 1 270 IF P gt OR P OR P OR P THEN 290 280 GOTO 250 290 IF LEN S 5 THEN RETURN 300 I 3 310 IF ASC MID S I 1 13 THEN 330 320 I I41 GOTO 310 330 RESPO 340 RETUR The subroutine at 1 NSE MID S 3 1 3 N ines 150 340 is designed to be called after each transmission to the pump The pump terminates each line of its response with the two character sequence Carriage Return Line Feed See Appendix 1 for details Lines 310 320 search for the Carriage Return character ASCII 13 and line 330 extracts the response string 9 Example 4 Volume Dispensing Example 4 uses the same subroutine as the last example and demonstrates the volume dispensing capabilities of the pump Several useful programming techniques are also illustrated 10 REM E 20 CLS K 30 OPEN 32 PRINT 36 PRINT 38 PRINT 40 PRINT 42 INPUT 44 IF Kg 46 IF Kg 48 GOTO 50 PRINT 52 PRINT XAMPLE4 EY OFF COM1 1200 N 8 2 RS CS DS AS 1 1 STP GOSUB 150 1 RAT GOSUB 150 RATE RESPONSE 1 RNG GOSUB 150 RANGE RESPONSE Current rate is RATE RANGE Change Rate Y or N K Y OR K y THEN 50 N
3. A maximum of ten Model 22 s can be remotely controlled from a single computer RS 232 port by assigning to each pump a unique address from 0 to 9 which will serve as the pump s identifier In addition Model 22 s can be inter mixed on the same daisy chain with other daisy chainable pumps from Harvard Apparatus Note Each pump in tbe chain must bave a unique address Keyboard Operation To assign an address identifier to a pump press SET and 0 Publication 5385 003 REV A E Harvard Apparatus Syringe Pump Model 22 Computer Control of Multiple Pumps Contd The display will show Ad n where n is a number from 0 to 9 indicating the pump s current identifying address Press the number from 0 to 9 representing the pump s address The number in the m display represented by n above will change to the number pressed Press ENTER when the desired address is displayed Remote Operation When transmitting a command to the pump precede each command with the address of the pump For example the following command will request a pump with address 1 to run 1 RUN CR Only the pump whose address matches the address preceding the command will respond to the command all other pumps will ignore the command Only pumps which have recognized its address on a command will enter remote mode indicated by the REMOTE lamp illuminating When a pump responds to a command preceded by its address it terminates its response w
4. O R oO gt x o a E 1 Connector Female 25 pin D connector on rear panel RS 232 Lines Pin 1 GND Pin 2 RXD Receive data from computer input Pin 3 TXD Transmit data to computer output Pin 7 GND Pin 19 TXD Transmit data to daisy chain output Pin 21 RXD Receive data from daisy chain input Make no connection to other pins for RS 232 interface A normally open momentary switch may be connected between Pin 18 and Pin 7 A momentary closure will start the pump if it is stopped and stop it if it is running This interface should not be used in conjunction with RS 232 Publication 5385 003 REV A 26 Harvard Apparatus Syringe Pump Model 22 Appendix D Contd 2 Baud Rate Baud rate may be set to 300 1200 2400 or 9600 baud The setting is preserved in nonvolatile memory To set baud rate Press SET and START STOP key simultaneously The display will show the current baud rate Each time START STOP is pressed the display will change showing 300 1200 24 2400 or 96 9600 Pressing ENTER will select the currently displayed baud rate The display will return to its normal condition displaying flow rate Front Panel Indicator REMOTE lamp indicates that pump is under remote control When lit the key board is disabled Indicator lamps and the numeric display continue to function when under remote control Communication RS 232 format is No Parity i Start 8 Data bits 2 S
5. eg 50 ml min with a 10 microliter syringe the pump will reject this instruction and notify the user Nonvolatile Memory The pump remembers its last instructions entered from the keyboard including diameter and flow rate even after the power has been disconnected Non Flow Detection An optical sensor detects lack of motion due to jamming end of syringe travel kink ing of tubing etc shuts down the pump and alerts the user Momentary Loss of Power The pump will stop and alert the user RS 232 Interface The pump is provided with a built in interface for use with most laboratory comput ers A Users Manual for RS 232 application is enclosed TTL Logic The pump can be stopped started and reversed remotely Publication 5385 003 REV A sf Harvard Apparatus Syringe Pump Model 22 Initial Set Up 1 Read the Manual 2 Locate the voltage selector switch on the rear panel of the pump see below and set it to the voltage being used If other than 115V 60 Hz is being used the plug must be cut off and an appropriate plug installed observing the polarity of the international line cord used Brown high Blue neutral Green ground 3 Turn on main power switch located directly above the line cord on the rear panel The display will now illuminate indicating that the power connections are correct The flashing number displayed is the current flow rate Flashing indicates that power has just been applied N Pa
6. 24 397 0 1000 ul 0 048 795 0 1 ml 0 049 805 0 2 ml 0 011 186 6 2 ml 0 010 168 2 3ml 0 011 181 4 5 ml 0 019 317 0 10 ml 0 028 461 0 20 ml 0 050 821 0 30 ml 0 074 1208 0 50 ml 0 002 28 40 100 ml 0 003 47 60 140 ml 0 004 55 10 Publication 5385 003 REV A 238 Harvard Apparatus Syringe Pump Model 22 Appendix C Model 22 Interface Command Reference After each transmission to the Pump terminating with a CR character ASCII 13 the pump enters remote mode and responds with the three character sequence CR LF prompt The prompt character indicates the status of the pump as follows When stopped ASCII 58 decimal When running forward ASCII 62 decimal When running reverse ASCII 60 decimal When stalled ASCII 42 decimal Note With a pump chain if no address precedes the command transmitted the pump at address 0 will respond to tbe command Commands Response CR LF prompt KEY Return to keyboard control Remote lamp off RUN Infuse forward direction REV Start reverse direction STP Stop CLV Clear volume accumulator to zero CLT Clear target volume to zero dispense disabled MLM number Set rate units are milliliters per minute ULM number Set rate units are microliters per minute MLH number Set rate units are milliliters per hour ULH number Set rate units are microliters per hour MMD number Set diameter units are mm Rate is set to 0 MIT number Set target infus
7. 9 13 6 30 21 70 12 50 60 26 70 20 35 60 140 Air Tite All Plastic Size Diameter 5 12 45 Size Diameter 10 15 90 0 25 cc 3 45 mm 50 29 00 2 8 92 3 8 99 5 11 70 Unimetrics 10 14 70 Series 4000 amp 5000 20 1958 30 22 70 Size Diameter 50 29 00 10 ul 0 460 mm 100 35 70 25 0 729 50 1 031 100 1 460 250 2 300 500 3 260 1000 4 610 Publication 5385 003 REV A Terumo Diameter 8 95 mm 13 00 15 80 20 15 23 10 29 10 Sherwood Monoject Plastic Diameter 4 65 mm 8 94 12 70 15 90 20 40 23 80 26 60 38 40 Popper amp Sons Inc 25cc 960mm Perfektum Glass SGE Scientific Glass Engineering Size Diameter 25 ul 0 73 mm 50 1 03 100 1 46 250 2 30 500 3 26 1 0ml 4 61 mm 2 5 7 28 5 10 30 10 14 57 Hamilton Microliter Series Gastight Size 0 5 ul 1 2 5 10 25 50 100 250 500 1 0 ml 2 5 10 25 50 Diameter 0 103 mm 0 1457 0 206 0 3257 0 460 0 729 1 031 1 46 2 3 3 26 4 61 mm 7 28 10 3 14 57 23 0 32 6 Appendix B 2 Nominal Minimum amp Maximum Flow Rates Various Syringes Actual Limits Will Vary Depending on Manufacturer Model 22 SYRINGE puL Hour uL Minute ml Hour ml M SIZE Min Max Min Max Min Max Min Max 0 5 ul 0 002 23 8 1 pl 0 003 47 8 2 ul 0 006 95 2 Harvard Apparatus Syringe Pump Model 22 5 ul 0 015 238 0 10 pl 0 029 474 0 25 ul 0 073 1193 0 50 ul 0 002 39 7 100 ul 0 005 79 7 250 ul 0 012 197 8 500 pl 0 0
8. Condition Outputs Available Pin 15 High output 2 5 5 0 volts when pump running Low output 0 0 5 volts when pump is stopped or stalled Forward Reverse Pin 16 High Normal Running in Infusion Low Short to ground pump reverses and withdraws Remote START STOP Timer Pin 17 High Normal Running Low Short to Ground Pump Stops Remote START STOP Momentarily Footswitch Pin 18 When momentary shorted to ground if pump is stopped it starts if pump is running it stops Note that pump will not respond to a command of less than 50 millisecond duration Power amp Ground Pin 1 Ground Pin 7 Ground Pin 14 5 Volts in series with 47 ohm resistor Current drawn should not exceed 25 milliamps Non TTL Pin Functions Pins 1 Used for RS 232 computer control Pins 2 Used for RS 232 computer control Pins 3 Used for RS 232 computer control Pins 7 Used for RS 232 computer control Pins 19 Used for RS 232 computer control Pins 21 Used for RS 232 computer control Publication 5385 003 REV A Harvard Apparatus Syringe Pump Model 22 RS 232 Tutorial Tutorial Contents Tutorial Contents Contd 1 Introduction 9 Example 4 Volume Dispensing 2 Computer to Pump Connection 10 Example 5 Ramping Flow Rate 3 Setting Baud Rate 11 Other Languages 4 Starting BASIC 5 Example 1 Terminal Emulator Appendix I Model 22 Interface 6 Keyboard Commands via RS 232C Command Referen
9. ET and RATE keys simultaneously One of the four range lamps will flash Each successive press of the RATE key will advance the flow rate range Stop when the desired range is flashing c Enter the numerical value of the flow rate desired including decimal point if you make an error enter numbers to clear the display When the correct flow rate value is displayed press ENTER The pump is now programmed for flow rate The display will indicate the numerical value and the units of flow rate will be illuminated Publication 5385 003 REV A s m Harvard Apparatus Syringe Pump Model 22 Operating the Pump Contd Start pump by pressing STOP START key and the pump will run and the RUN light will be illuminated Stop pump by pressing STOP START key and the RUN light will go out If SET and START STOP are pressed simultaneously the baud rate is displayed and the remote lamp will flash Press ENTER to return to normal operation The remote lamp when illuminated indicates that the pump is under remote computer control These features are covered in detail in the RS 232 instructions Out of Range Certain combinations of syringe size and flow rate are outside the range of the pump such as asking for ul hour with a 100 ml syringe The pump will reject this input data when the ENTER key is pressed and the display will read oor until a dif ferent combination of syringe and rate that is within range is entered See Table 2 for typical maxi
10. K KK 160 S P RESPONSE Publication 5385 003 REV A i9 Harvard Apparatus Syringe Pump Model 22 RS 232 Tutorial Contd 170 WHILE P lt gt AND P lt gt gt AND p AND p lt gt x 180 IF LOC 1 gt 0 THEN S S INPUT LOC 1 1 190 P RIGHT S 1 200 WEND 210 IF LEN S gt 4 THEN RESPONSE MID S 3 INSTR 3 S CHR 13 3 220 RETURN Lines 54 and 74 test the extreme rates if they are within range then the intermediate rates will be acceptable R is the range command string Note the use of the PRINT 1 USING statement in lines 52 and 70 to allow rate setting in any one of the four ranges Line 84 sets up a timer interrupt which causes the subr outine at line 100 to be executed one per second The interrupt is turned on and off with the TIMER statements The variables P and T are tested by the main program and modified by the interrupt subroutine Line 120 computes RATE as a function of the current time T the duration TFINAL and the initial and final rates Other flow rate functions can be programmed by changing this line The subroutine at line 150 is a more elegant version of the get response routine using a WHILE WEND loop and the INSTR function Its behavior is identical to the earlier version 11 Other Languages Any language which supports RS 232 communication may be used to control the pump Computer Control of Multiple Pumps Daisy Chain Option
11. Model 22 Syringe Pump Series User s Manual Model 22 Syringe Pump MAI 55 2222 Model 22 Infuse Withdraw MAI 55 2226 Model 22 Multisyringe MAI 55 5920 HARVARD APPARATUS Publication 5385 003 REV A WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will
12. OR K n THEN 60 42 H1 KEY GOSUB 150 Change rate from pump keypad Publication 5385 003 REV A ixl Harvard Apparatus Syringe Pump Model 22 RS 232 Tutorial Contd 54 PRINT Press any computer key to continue 56 IF INKEY THEN 56 58 GOTO 32 60 INPUT Enter volume to dispense ml VOLUME 62 PRINT 1 USING MLT VOLUME GOSUB 150 64 IF RESPONSE THEN 66 65 PRINT Error Try again GOTO 60 66 PRINT 1 CLV GOSUB 150 68 PRINT 1 RUN GOSUB 150 70 PRINT 1 VOL GOSUB 150 72 PRINT Volume dispensed RESPONSE 74 IF P gt THEN 70 80 IF P THEN 90 82 PRINT Done PRINT GOTO 32 90 PRINT Stalled PRINT GOTO 32 150 REM kkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkxk 160 REM get response subroutine 170 REM ck ck ck ck ce ke ce ee ce ce ke ck ck ke ce ke ce ck ce ck ck ce ck ck ck ko ko ko ko kc ko KK 180 REM returns 190 REM P prompt character gt lt or x 200 REM RESPONSE response string if syntax error 210 REM OOR if out of range 220 REM if prompt only 230 REM 240 S RESPONSE 250 IF LOC 1 gt 0 THEN S S INPUT LOC 1 1 260 P RIGHT S 1 270 IF P gt OR P OR P OR P THEN 290 280 GO TO 250 290 IF LEN S 5 THEN RETURN 300 I 3 310 IF ASC MID S I 1 13 THEN 330 320 I I41 GOTO 310 330 RESPONSE MID S 3 1 3 340 RETURN The subrout
13. R R MLH OR R ULM OR R ULH THEN 50 42 IF R mlm OR R mlh OR R ulm OR R ulh THEN 50 46 PRINT Invalid Range GOTO 34 50 INPUT Enter Final Rate FRATE 52 PRINT 1 USING R H H H FRATE s 54 GOSUB 150 IF RESPONSE THEN 66 62 PRINT Out of Range GOTO 50 o 66 INPUT Enter Initial Rate IRATE c 70 PRINT 1 USING R HHHH IRATE z 74 GOSUB 150 IF RESPONSE THEN 80 I 78 PRINT Out of Range GOTO 66 80 PRINT 1 RNG GOSUB 150 RANGE RESPONSE 82 INPUT Enter Ramp Duration Seconds TFINAL 83 PRINT PRINT Press STOP START on pump to interrupt 84 ON TIMER 1 GOSUB 100 85 T O PRINT 1 run GOSUB 150 86 TIMER ON 90 IF Pg gt THEN 94 92 TIMER OFF PRINT Interrupted PRINT GOTO 22 94 IF T TFINAL THEN 90 95 TIMER OFF 96 PRINT Done PRINT GOTO 22 100 REM kkkkkkkxkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkxkxk 104 REM timer subroutine executed once a second 106 REM kkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkxkxk 110 T T 1 120 RATE IRATE T FRATE IRATE TFINAL 122 PRINT USING t iHHHHH HHHH HHHH RANGES T RATE 130 PRINT 1 USING R H H RATE 140 GOSUB 150 141 PRINT 1 KEY GOSUB 150 142 RETURN 148 REM Xkkckckck ck ckck ck ck ck ckckck ck ckckckckckckckckckck KKK KKK KK KKK KK 150 REM get response subroutine version 2 152 REM Xkokckckck ck ckck ck ck ck ck ck ck ck ckckckckckckckckckck ckckckckck ck ck kc KK
14. TART key Maintenance Model 22 requires no special maintenance other than keeping it clean by avoiding accidental spills of pumped material The two guide rods and the lead screw should be sparingly lubricated periodically with the Magnalube G R grease provided with the pump This Teflon R based grease is available either from Harvard Apparatus or Carleton Stuart Corp 13 02 44th Ave Long Island City New York 11101 Solvents of any type should never be used to clean the pump mild detergent solution may be used to clean the key pad Publication 5385 003 REV A s Harvard Apparatus Syringe Pump Model 22 Remote Control All new Model 22 Pumps are equipped with both TTL Logic and a built in RS 232 Interface via the 25 pin connector at the rear TTL Logic Transistor to Transistor Logic The TTL Logic exists in all pumps manufactured after April 1 1988 with serial numbers A 24090 or higher TTL is a system of control in which there are only two states HIGH In which a voltage of 2 5 5 0 volts is applied to a pin LOW In which a TTL voltage of 0 0 5 volts is applied In this application low is a short circuit to ground n v 8 1 15 1 5 1 58 17 2 21 22 79 M 35 Note This view is from witbin tbe pump looking outwards tbroutb tbis Connector All pin numbers refer to the 25 pin D Sub connector Publication 5385 003 REV A Harvard Apparatus Syringe Pump Model 22 TTL Functions Pump
15. ay RS 232C Interface Non Volatile Memory Publication 5385 003 REV A Microprocessor multiple syringe infusion or infusion withdrawal 140 x 220 x 220 cm 5 5 x 11 x 8 75 in 10 Ibs 4 5 kg 115 230 VAC 50 60 Hz via selector switch 45 W Typically 15 mA Typically lt 0 2 Q 95 to 130 VAC 50 60 Hz 220 to 260 VAC 50 60 Hz selectable 0 9 microprocessor controlled stepping motor 2 1 reduction from motor 24 thread inch 32000 1 4 stepping 6 8 second step 416 7 msec step 0 330729 um minimum 2 9068 m min 47 6 mm min 16 384 to 1 x 0 03576 0 05 Automatic enter syringe size up to 50 mm 0 5 ul to 140 ml 0 002 ul hr with 0 5 ul syringe 55 1 ml min with 140 ml syringe 3 1 2 digit numeric LED 7 LED indicators for range diameter Run Stop and remote control Chained dual bidirectional ports Storage of all settings 2 Harvard Apparatus Syringe Pump Model 22 Features The Model is an Infusion Only syringe pump employing a microprocessor controlled small step angle stepping motor driving a lead screw and half nut A key pad is used for data entry of flow rate and syringe diameter The microprocessor calculates the cross sectional area of the syringe to be used and calibrates the flow rate for that syringe A number of special features result from use of microprocessor technology Out of Range If the user selects a combination of syringe size and flow rate that is outside the range of the pump
16. cation 5385 003 REV A Declaration of Conformity Application of Council Directive s 73 23 EEC 89 336 EEC Standard s to which conformity is declared EN61010 EN50082 1 EN50081 1 Manufacturer s Name Harvard Apparatus Inc Manufacturer s Address 84 October Hill Road Holliston Massachusetts 01746 U S A Type of Equipment Syringe Pump Model No Model 22 I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place United States of America Date November 11 1996 A C Signature Beth Bauman HARVARD ees APPARATUS VP Engineering Operations Position
17. ce 7 Example 2 Handling the Pump Appendix II Interface Specification ae e s h for Model 22 Infusion xample 3 Interpreting the Pump Response Tutorial Step by Step 1 Introduction The purpose of this manual is to introduce users of Model 22 to the use of the built in RS 232C computer interface It is designed to assist those who are not familiar with computer control of laboratory instruments to write programs in BASIC on IBM PC or compatible computers Experienced programmers may prefer to skip to the technical specification in Appendix II The example programs are intended to show the fundamentals of communication to the instrument and to serve as building blocks when writing programs tailored for a specific application Each example program builds on the previous one They include programs that permit keyboard control of the pump and demonstrations of automatic volume dispensing and variable flow rate operation A pre wired cable for interface to IBM PC or compatible computers is available from Harvard Apparatus Order catalog number 55 2223 IBM to Model 22 RS 232C Cable Computer to Pump Connection The Model 22 Infusion Pump uses an RS 232C interface to communicate with a computer This is the same communication system used by most terminals and modems and many other laboratory instruments On the IBM PC and compatible computers this interface is usually called a serial or COM port The computer sho
18. continue to monitor the application of the RoHS Directive to its products and will comply with any changes as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required 2 Harvard Apparatus Syringe Pump Model 22 Table of Contents SUBJECT PAGE NO General Information Warranty and Repairs 2 LlrTdijecine cm M 3 Featur f 4 Initial Set UP Kc 5 Loading Syringes umsissssssennnnsannnsp nnksvemekevevvnn ne 6 Operating the Pump Entering Syringe Diameter s 7 Entering Flow Rate s ssssss 7 Out of Pange err irre rre ken ideni 8 Changing Flow Rates on eie ree testa 8 End Of MAV Ol atsvsrsccdseaventnsremensanavaeseavsx mme 8 Momentary Loss of Power 8 ETL RISO EEE 8 Remote el Qo e 9 TIL FUNGQUONS me 10 Non TTE Functions coe eru aenea ou Ease Ser peer nRxER EUR 10 H5 232 TULOFIA aiiis apaa eaa araara 11 Computer Control of Multiple Pumps 19 Appendices A Table of Syringe Diameters 21 B Nominal Minimum and Maximum Flow Rates 22 C Interface Command Reference 23 D Interface Specifications 25 E M lntenance u unmrus taus hv siv 27 F Accessories svoren 28 Publication 5385 003 REV A E Harvard Apparatus Sy
19. ers using the black thumb screws so that both the head of the pluncger and the flange on the barrel are held securely in place with minimal free play 22 Pumps with Multiple Syringe Holders Catalog Numbers 55 5920 and 55 2219 These versions of the Pump 22 have a saw tooth syringe holder capable of holding up to ten syringes emptying them simultaneously at identical rates This multiple syringe will accommodate up to Ten syringes of any size up to 10ml Six syringes of any size up to 50 ml Catalog Numbers 55 2314 and 55 2316 Have a multiple syring holder which accommodates up to four syringes of 30 ml up to 140 ml To load syringes rotate the spring loaded sponge rubber retainer arm out of the way and lay syringes in V shaped slots with the syringe flange touching the holder Rotate the retainer arm to hold syringes in place If less than the maximum number of syringes are used try to place them symmetrically about the lead screw to present a balanced load Note The back pressure for each syringe will be divided by tbe number of syringes used Thus for four 50 cc syringes tbe maximum pressure developed in eacb syringe will be 400 mm Hg 8 psi Publication 5385 003 REV A 7 Harvard Apparatus Syringe Pump Model 22 Operating the Pump The syringe diameter and flow rate must be entered into the pump s memory using the key pad and LED display See figure below Front panel indicating that pump i
20. eys simultaneously The display will show the current baud rate and the REMOTE lamp will blink Fach time the START STOP key is pressed the display will change showing 300 1200 24 2400 or 96 9600 Pressing ENTER will select the currently displayed baud rate The display will return to its normal condition displaying flow rate The REMOTE lamp will extinguish The Model 22 may be used with an IBM PC or compatible computer at any of these baud rates The fastest rate 9600 baud is recommended The examples assume that the pump has been set for 1200 baud The pump baud rate is preserved in non volatile memory it does not need to be reset each time the pump is turned on Starting Basic The serial interface in the IBM PCT or compatible computer contains the hardware necessary to communicate with the pump To control this hardware a program is required AII of the example programs are written in the BASIC language which is supplied with the operating system of the computer Depending on which kind of computer is used the operating system may be PC DOS Compaq DOS or MS DOS Whichever it is Version 2 0 or higher should be used The BASIC interpreter may be called GWBASIC or BASICA This tutorial assumes a rudimentary knowledge of programming in BASIC Refer to the manuals supplied with the operating system to learn how to start the BASIC interpreter edit BASIC programs run and debug programs and save and load programs on di
21. ied type and rating 3AG 1 2 AMP 250V SLO BLO FUSE VARNING USE IN MANNER NOT SPECIFIED BY THE MANUFACTURER MAY IMPAIR THE PROTECTION PROVIDED BY THE EQUIPMENT Publication 5385 003 REV A 28 Harvard Apparatus Syringe Pump Model 22 Appendix F Accessories For All Pumps Foot Switch For remote START STOP switch has 9 ft cable terminating in connector to fit 25 pin terminal at rear of pump Press switch to start press to stop Catalog No Product MA1 55 2215 Foot Switch only with cable and connector Reversing Switch This switch plugs into the 25 pin connector and changes the direction of motor rotation to produce either infusion or withdrawal It is the same switch that is supplied with infusion withdrawal pumps Catalog No Product MA1 55 2217 Reversing Switch only RS 232 Kit For IBM computers and compatibles Consists of cable with 25 pin connectors at each end to connect all pump 22 s to the RS 232 port Enables user to write programs to Control all functions remotely e Reversing Ramping up and down Control volume delivered Catalog No Product MA1 55 2223 RS 232 Cable for IBM compatibles MA1 55 2225 RS 232 Cable for non IBM compatibles Daisy Chained Control Catalog No Product MA1 55 2239 Daisy Chain Connector MA1 55 4145 Computer to Daisy Chain for IBM Computers MA1 55 4146 Computer to Daisy Chain for non IBM Computers MA1 55 7760 Daisy Chain to Daisy Chain Cable Publi
22. ine at lines 150 340 is called after each transmission to the pump This ensures that the pump has executed the command and finished transmitting its response before the program continues Each time a command is received by the pump the REMOTE lamp on the key pad illuminates and the pump key pad is locked out Line 50 illustrates use of the KEY command which allows the programmer to re enable the key pad Line 62 illustrates the best way to send commands with numeric arguments The PRINT 1 USING statement ensures that the argument is transmitted in a format which is acceptable to the pump The test at line 64 is necessary to detect out of range responses to the prior command If the target volume is out of range then RESPONSES will equal OOR The loop at lines 70 74 reads the pump s volume accumulator and tests the prompt to determine when the dispense is complete Line 80 detects the stall condition if the pump has reached the end of its travel Publication 5385 003 REV A RS 232 Tutorial Contd 18 7 10 Example 5 Ramping Flow Rate a A useful feature of Model 22 is its ability to change rate without stopping x Example 5 illustrates variable flow rate infusion 3 10 REM EXAMPLES 14 KEY OFF CLS o 18 OPEN COM1 1200 N 8 2 RS CS DS AS 1 E 22 PRINT 1 STP GOSUB 150 n 26 PRINT 1 CLT GOSUB 150 34 INPUT Enter Range MLM MLH ULM OR ULH R 38 IF R MLM O
23. ion volume units are ml Numbers between 0 and 1999 will be accepted by the pump Leading zeros and trail ing decimal point are optional Any number of digits to the right of the decimal point may be transmitted The number received will be rounded to four significant digits if the leading digit is 1 or three significant digits if the leading digit is 2 to 9 Queries Response CR LF value CR LF prompt Queries with Numeric Response DIA Send diameter value units in mm RAT Send rate value in current range units VOL Send current accumulated infused volume units are ml TAR Send target volume units are ml VER Send model and version number currently 22 900 Value format nnnn nnn The transmitted value is an 8 character string with leading zeros converted to SP characters ASCII 32 The fifth character is a decimal point ASCII 46 Publication 5385 003 REV A Appendix C Contd 24 Query Response CR LF range CR LF prompt Queries with String Response RNG Send range message Range is a character string one of ML H MI M UL H or UL M Error responses CR LF CR LF prompt Unrecognized command CR LF O O R CR LF prompt Out of range Harvard Apparatus Syringe Pump Model 22 Publication 5385 003 REV A Appendix D 25 7 Interface Specification for Model 22 Infusion Pump a N i RS 232 Cable Wiring o o e E 2 n o y 2 z 2 gt 2 D o E e 2 oO amp R e K lt A
24. ith its prompt character preceded by the pump s address For example with the above command the pump will respond with CR LF 1 To just request a pump s current prompt send just the pumps address 1CR The addressed pump will respond with its prompt preceded by its address By default if the pump address is omitted from any command address 0 will be used and the pump with address O will respond to the command but will not return its address before the prompt Pump Daisy Chain to Computer Connection For each Model 22 in the Daisy Chain Attach a 25 Pin to Daisy Chain Adapter 55 2239 to the RS 232 Connector on the rear of each pump in the Daisy Chain Attach a 25 Pin to Daisy Chain Cable 55 4145 for IBM and compatibles or 55 4145 for other computers from the computer s RS 232 port to the socket labeled IN on the Daisy Chain Adaptor of the first pump For each additional pump in the Daisy Chain after the first pump attach a Daisy Chain to Daisy Chain Cable 55 7760 from the socket labeled OUT on the previous pump to the socket labeled IN on the next pump Publication 5385 003 REV A Appendix A Syringe Diameters in mm a Stainless Steel Size Diameter Size 8 CC 9 525 mm 3 CC 20 cc 19 130 5 50 cc 28 600 10 100 cc 34 900 20 30 60 Becton Dickinson Plastic Plasti pak Size Diameter Harvard Apparatus Syringe Pump Model 22 1 cc 4 78 mm 3 8 66 Size 5 12 06 re 10 14 50 3 20 1
25. mp in line 50 Line 50 also sends an end of line character Carriage Return When the end of line character is received by the pump it interprets the preceding characters and sends its response The response will depend on the particular command received but it will always end with one of the four prompt characters which indicate its status Pump Stopped Pump Infusing Pump Reversing Pump Stalled Line 80 sets P to the last character received The program continues concatenating characters to S until a prompt is received 8 Example 3 Interpreting the Response Example 3 demonstrates a method of breaking up the pump response transmission into its constituent parts 10 REM EXAMPLE3 20 CLS 30 OPEN COM1 1200 N 8 2 RS CS DS AS 1 40 INPUT Enter Command COMMAND 50 PRINT 1 COMMAND 60 GOSUB 150 70 PRINT Response RESPONSE 80 PRINT Status HE 90 IF P THEN PRINT STOPPED 100 IF P gt THEN PRINT RUNNING 110 IF P lt THEN PRINT REVERSING Publication 5385 003 REV A 16 7 Harvard Apparatus Syringe Pump Model 22 RS 232 Tutorial Contd 120 IF P THEN PRINT STALLED 130 PRINT 140 GOTO 150 REM 160 REM 170 REM 40 koc ck ckecke ke ke kk ck ck ck e e ke ke ek kk ck ke kx kc k kk ck oko k ck kock get response subroutine ck ck ckcke ke ke kk ck ck ck ee ke ke ek kk ck ke kx Sk kk kc k ko ko koc k ok 180 REM returns 190 REM P prompt character 2
26. mum and minimum rates for various size syringes Changing Flow Rate To change the flow rate while the pump is running press SET and RATE simultane ously The display will go blank Enter the new flow rate The pump will continue to run at the old flow rate until the ENTER key is pressed at which time the pump will run at the new rate End of Travel The pump motor is equipped with an optical rotation sensor If for any reason an electrical step signal to the motor is not followed by a mechanical step of the motor the power to the motor is shut down and the RUN light will flash indicating the pump is stalled This can occur when the syringe plunger hits bottom a line is kinked syringe plunger binding or any situation requiring more force to the head of the syringe than the pump is capable of delivering To restart the pump remove the cause of stalling and press START The pump has an adjustable collar on the rear guide rod This collar can be pre set to terminate pumping at any point It is also useful when small microliter syringes are being used since the pusher block can bend the tiny wire plungers Set the collar to stop the pump before the microliter plunger hits bottom Momentary Loss of Power If during the course of pumping the A C power has been interrupted the pump will stop running and will not resume when the power goes on This condition results in the display flashing and the RUN light going out To resume pumping press the S
27. o the string variable S using the INPUT function in line 80 Line 90 displays the received characters and the program loops back to line 40 Keyboard Commands Via RS 232 To test the program and interface Turn off the pump Turn it on again the pump display should be blinking Run the program Publication 5385 003 REV A 14 7 Harvard Apparatus Syringe Pump Model 22 RS 232 Tutorial Contd At this point nothing will appear on the monitor since the program is looping continuously waiting until either a key is pressed or a character is received from the pump To make something happen press the enter RETURN key of the computer keyboard The following should occur The REMOTE lamp of the pump illuminates The pump display stops blinking m The character appears on the computer monitor What has occurred is that the character corresponding to the enter key Carriage Return has been read by the program and sent to the pump The pump has responded with a prompt character which indicates that it is stopped This has been received by the program and output to the computer display The program is still looping waiting for the next event to occur If the pump and computer do not behave as described make sure that the cable is properly connected to COMI and that the pump baud rate is set to 1200 Make sure your cable is wired according to Figure 1 If you are using COM2 or are not sure which port
28. returned materials authorization number has been issued by our cus tomer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practi cal enclose a sample or drawing We offer a complete reconditioning service CAUTION This pump is not registered with the FDA and is not for clinical use on human patients CAUTION NOT FOR CLINICAL USE ON HUMAN PATIENTS Publication 5385 003 REV A if Harvard Apparatus Syringe Pump Model 22 Specifications Specifications Type Size HxWxD Weight Power Leakage to Ground Ground Resistance Voltage Operating Range Drive Motors Timing Belt Drive Lead Screw Pitch Motor Steps per 1 Revolution of Lead Screw Step Rate Minimum Maximum Pusher Advance Step Pusher Travel Rate Minimum Maximum Dynamic Speed Range Accuracy Reproducibility Calibration Syringe Size Flow Rate Range Minimum Maximum Displ
29. ringe Pump Model 22 General Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency While the flow will stay calibrated the peak will vary Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse nor mal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCI DENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclu sion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied war ranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 1371 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA
30. s running at 22 2 ml minute under remote control from a computer Tbe display reads 22 2 while tbe ml min unit light remote and the run light are illuminated 1 Entering the Syringe Diameter a Identify the make and capacity of the syringe to be used using the diameter table on page 21 or attached to the line cord Referring to this syringe diame ter table determine the diameter in mm If your syringe is not listed deter mine the diameter of the plunger using a micrometer and note it for future reference b Depress the SET and DIAM keys simultaneously This procedure prevents accidental changes The mm diam light will flash indicating that the pump is ready to receive input c Using the key pad enter the syringe diameter complete with decimal point watching the result on the display If you make an error continue entering numbers until the display goes blank and then enter the correct value d When the display reads the desired diameter press ENTER The mm diame ter indicator light will go out The syringe diameter has now been entered into memory Each time a diameter is programmed the rate is set to zero To recall the syringe diameter entered at any time even while the pump is running simply press DIAM and the diameter will appear on the display without affecting flow while the key is depressed 2 Entering Flow Rate a Determine the range of flow rates ml min ml hour ul min or ul hour for your use b Press S
31. sed by subsequent statements to refer to this interface There may be other OPEN statements in a program for other interfaces or disk files if so they should use a different file number The particular RS 232C port is identified by COM If your computer has two ports then COM2 may also be used The statement sets the COM1 baud rate to 1200 and defines the particular format of serial bits to be used With Model 22 this Is no parity bit eight data bits per character and two stop bits Line 30 clears the bottom line of the screen and turns on the flashing cursor Line 40 reads the keyboard and assigns a single character string to string variable K if a key has been pressed This statement does not wait for a keystroke if no key has been pressed then the null string is assigned to K Line 50 sends the character to the display and line 60 sends it to file 1 which has been assigned to COM1 These statements connect the computer keyboard to both the display and the pump connected to COMI If no key is pressed then K is the null string nothing is displayed or transmitted and the program continues execution Line 70 uses the LOC file number function to determine if any characters have been received from the interface If the value of the function is less than one then no characters have been received and the program loops back to line 40 continuously testing the keyboard If characters have been received then they are transferred t
32. skettes The RS 232C interface operates one bit at a time However the interface is character oriented from the programmer s point of view Programs send characters to the interface and receive them from the interface in the same way they accept characters from the keyboard and display them on the monitor Each operation is controlled independently by the program which is running Example 1 demonstrates how to tie the keyboard display and interface together so that the data transmission can be controlled and observed Publication 5385 003 REV A RS 232 Tutorial Contd 13 7 Harvard Apparatus Syringe Pump Model 22 55 Example 1 Terminal Emulator Start the BASIC interpreter At the OK prompt type in EXAMPLEI 10 REM EXAMPLE1 20 OPEN COM1 1200 N 8 2 RS CS DS AS 1 30 KEY OFF LOCATE 1 0 7 40 K INKEY 50 PRINT K 60 PRINT 1 K 70 IF LOC 1 1 THEN 40 80 S INPUT LOC 1 1 90 PRINT S 100 GOTO 40 This sort of program is usually called a terminal emulator since it connects the key board display and RS 232C interface so that they function similarly to a computer terminal Its use here is to permit testing and experimentation with the Model 22 interface The OPEN statement in line 20 initializes the interface and assigns it a file number An OPEN statement must be executed once before attempting to use an interface The file number 1 in this case may be any value from 1 to 255 and will be u
33. top bits Transmissions to the pump consist of sequences of ASCII characters terminated with CR carriage return ASCII 13 decimal Upper case ASCII 64 to 95 decimal and lower case ASCII 96 to 127 decimal are equivalent ASCII characters from 0 to 12 and 14 to 31 are ignored SP space ASCII 32 decimal is ignored Whenever a transmission is received the pump enters remote mode illuminates the REMOTE lamp and disables the keyboard Publication 5385 003 REV A a7 Harvard Apparatus Syringe Pump Model 22 Appendix E Maintenance Model 22 requires no special maintenance other than keeping it clean by avoiding accidental spills of pumped material The two guide rods and the lead screw should be sparingly lubricated periodically with the Magnalube G R grease provided with the pump This Teflon R based grease is available from Harvard Apparatus Catalog MA1 0606 086 Teflon R based grease Solvents of any type should never be used to clean the pump A mild detergent solution may be used to clean the keypad Fuse Replacement Make sure power cord is disconnected from main supply before replacing fuse Remove bottom cover on pump by removing four 4 rubber feet and two 2 screws Locate fuse on power supply module Remove fuse from fuse clip Use caution not to break the fuse when removing Replace fuse bottom cover screws and rubber feet For continued fire protection replace fuse only with 250V fuse of the specif
34. uld have one or two 25 position pin male connectors on the rear panel Note Any 25 position socket female connectors on the computer are parallel printer interfaces that are not suitable for controlling the pump Care should be taken to ensure that the male end of the pump cable is not connected to the computer The Model 22 pump has a 25 position socket female connector The cable wiring for use with a standard IBM PC or compatible serial port is shown in Figure 1 To connect the pump to the computer Turn off both devices Connect the socket end of the cable to the computer Connect the plug end to Model 22 Tighten the retaining screws Boot up the computer and turn on Model 22 Publication 5385 003 Revision A ial Harvard Apparatus Syringe Pump Model 22 RS 232 Tutorial Contd Once the cable is connected both the computer and the pump may be used normally Either one may be turned on or off at any time It is good practice however to turn off power when connecting or disconnecting the cable 3 Setting the Baud Rate RS 232 is a bidirectional serial data communications interface This means that in each direction computer to pump and pump to computer data is transmitted one bit at a time The rate of data transmission in bits per second is the baud rate The Model 22 will operate at baud rates of 300 1200 2400 or 9600 To set the baud rate Press SET and START STOP k
35. you are connected to change line 20 to 20 OPEN COM2 1200 N 8 2 RS CS DS AS fl and try again Assuming that the computer and pump are responding correctly type RUN enter The pump will begin to infuse and the character gt will be displayed Try some other commands from the table in Appendix I The computer monitor will appear similar to Figure 2 Since this program loops continuously press the CTRL and BREAK keys together to stop it Keyboard Command Script GW BASIC 2 02 C Copyright Microsoft 1983 1984 60892 Bytes free OK load example 1 OK run run gt gt rat 999 000 gt rng UL M gt ulm 123 4 gt rat 123 400 gt stp Publication 5385 003 REV A 1 7 Harvard Apparatus Syringe Pump Model 22 RS 232 Tutorial Contd 7 Example 2 Handling the Pump Response The character by character technique used in Example 1 is suitable for keyboard entry of commands however program control of the pump requires that a program wait until a complete response has been received Example 2 illustrates how to do this 10 REM EXAMPLE2 20 OPEN COM1 1200 N 8 2 RS CS DS AS 1 30 KEY OFF 40 INPUT Enter Command K 50 PRINT 1 K 60 S 70 IF LOC 1 gt 0 THEN S S INPUT LOC 1 81 80 P RIGHT S 1 90 IF P OR P gt OR P OR P THEN 110 100 GOTO 70 110 PRINT The Pump Response is S 120 GOTO 40 Line 40 accepts a string which is sent to the pu
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