QuickStix™ Kit for
Contents
1. grounds Waring blender or equivalent 6 Weigh sample into the appropriate size glass Mason jar and attach jar adapter with blade 7 Place assembly on the Waring blender or equivalent Shield with protective cover to prevent injury in the event of jar breakage Grind sample at high speed for 15 45 seconds or until all whole grains QuickStix for QuickScan Roundup Ready Corn Bulk Grain Page 2 of 4 Rev 03 21 11 USDA References http archive gipsa usda gov reference library handbooks grain insp grbook1 bk1 pdf USDA Grain Inspection Handbook Book 1 Grain Sampling http archive gipsa usda gov biotech sample2 htm Guidance document entitled Sampling for the Detection of Biotech Grains http archive gipsa usda gov biotech sample1 htm Practical Application of Sampling for the Detection of Biotech Grains http archive gipsa usda gov biotech samplingplan1 xls This website provides a simple to use Sample Planner 29K Excel Spreadsheet The planner allows you to enter different assumptions in terms of sample size number of samples acceptable quality level and to determine the probability of accepting lots with given concentration levels It also plots the probabilities in graph form for easy interpretation Specific data can be saved for documentation and future analyses Corn Common Extraction Grams of Corn x 1 5 mL of water For example 400 kernels with an average2. prior to preparation of each sample Use a new transfer pipette and reaction cup for each sample How to Run the QuickStix Strip Test 1 Allow refrigerated canisters to come to room temperature before opening Remove the QuickStix Strips to be used Avoid bending the strips Reseal the canister immediately 2 Place the strip into the reaction cup provided being sure to insert the end indicated by the arrows on the protective tape The sample will travel up the strip 3 Allow the strip to develop for 5 minutes before making final assay interpretations Positive sample results may become obvious much more quickly 4 Immediately cut off and discard the bottom section of the strip covered by the arrow tape and place in the QuickScan Reader Strips must be read while still wet NOTE Use extreme caution to prevent sample to sample cross contamination with grain fluids or disposables Interpreting the Results Development of the Control Line within 5 minutes indicates that the strip has functioned properly Any strip that does not develop a Control Line should be discarded and the sample re tested using another strip Results are scanned and interpreted quantitatively with the QuickScan System Place QuickStix Strip into the carrier slide in and press Read Test on the screen QuickScan will return a result as GMO or lt LOQ less than the Limit of Quantification Please consult the QuickScan User Manual fo
3. seed weight of 0 3 g 400 x 0 3 120 g of corn 120 g x 1 5 180 mL water Transfer 20 mL extract to cup Either pour or pipette to the 20 mL mark Bunn grinder or equivalent cont 8 Place subsample into an appropriately sized jar or zip type plastic bag and add the volume of tap water calculated using the Corn Common Extraction formula left 9 Cap the jar or zip plastic bag and shake vigorously for 30 seconds then allow sample to settle for another 30 seconds Waring blender or equivalent cont are broken Optimum grind time may vary based on sample size and condition of equipment The sample should be the consistency of coffee grounds 8 Add the volume of tap water calculated using the Corn Common Extraction formula left 9 Cap the jar and shake vigorously for 30 seconds then allow the sample to settle for another 30 seconds 10 Transfer 20 mL of the liquid portion from above the settled sample into the sample cup Pour extract into cup to the 20 mL line or use a fresh pipette from the kit to transfer extract until the 20 mL line is reached when testing smaller subsamples Important Avoid transferring particles as much as possible and after transfer allow the liquid in the sample cup to settle for 30 seconds so that any particles will settle at the bottom of the cup 11 To prevent cross contamination thoroughly clean blender parts and jars to remove dust and residue
4. QuickStix Kit for Roundup Ready Corn Bulk Grain Rev 03 21 11 Highlights Use with Common Extraction method Quantitative results in just over 5 minutes Available as 100 strip kits in bulk packaging or in QuickCombs Contents of Kit 100 QuickStix Strips packed in two moisture resistant canisters 100 transfer pipettes 100 reaction cups Items Not Provided Waring blender model 31BL91 or equivalent with glass jar adapter Eberbach E8495 and glass Mason jars OR Bunn grinder or equivalent Graduated cylinder Tap water Protective cover for blender jar while grinding QuickScan System Catalog Number AQ 010 BG Intended Use The QuickStix Kit for QuickScan Roundup Ready detects and quantifies CP4 EPSPS protein at the levels typically expressed in Roundup Ready corn The Limit of Detection for these QuickStix Strips is 0 5 Event NK603 corn i e one Event NK603 Roundup Ready corn kernel in 200 conventional corn kernels This kit will not detect GA21 Roundup Ready corn How the Test Works In order to detect the CP4 EPSPS protein expressed by Roundup Ready corn the sample must first be extracted to solubilize the protein Each QuickStix Strip has an absorbent pad at each end The protective tape with the arrow indicates the end of the strip to insert into the reaction cup The sample will travel up the membrane strip and be absorbed into the larger pad at the top of the st
5. Y OR FITNESS FOR A PARTICULAR PURPOSE The warranty provided herein and the data specifications and descriptions of EnviroLogix products appearing in EnviroLogix published catalogues and product literature are EnviroLogix sole representations concerning the Products and warranty No other statements or representations written or oral by EnviroLogix employees agents or representatives except written statements signed by a duly authorized officer of EnviroLogix Inc are authorized they should not be relied upon by the customer and are not a part of the contract of sale or of this warranty EnviroLogix does not warrant against damages or defects arising in shipping or handling or out of accident or improper or abnormal use of the Products against defects in products or components not manufactured by EnviroLogix or against damages resulting from such non EnviroLogix made products or components EnviroLogix passes on to customer the warranty it received if any from the maker thereof of such non EnviroLogix made products or components This warranty also does not apply to Products to which changes or modifications have been made or attempted by persons other than pursuant to written authorization by EnviroLogix THIS WARRANTY IS EXCLUSIVE The sole and exclusive obligation of EnviroLogix shall be to repair or replace the defective Products in the manner and for the period provided above EnviroLogix shall not have any other obligation with respe
6. ct to the Products or any part thereof whether based on contract tort strict liability or otherwise Under no circumstances whether based on this Limited Warranty or otherwise shall EnviroLogix be liable for incidental special or consequential damages This Limited Warranty states the entire obligation of EnviroLogix with respect to the Products If any part of this Limited Warranty is determined to be void or illegal the remainder shall remain in full force and effect License EnviroLogix has developed this kit using proprietary reagents as well as reagents licensed from Monsanto Company Roundup Ready is a registered trademark of Monsanto Technology LLC EnviroLogix the EnviroLogix logo QuickScan QuickStix QuickComb and Common Extraction are trademarks of EnviroLogix Inc EnviroLogix 2011
7. ng the working sample does not necessarily mean the originating lot is entirely negative for the analyte or protein in question Protect all components from hot or cold extremes of temperature when not in use Do not leave in direct sunlight or in a vehicle QuickStix for QuickScan Roundup Ready Corn Bulk Grain Page 4 of 4 Rev 03 21 11 For Technical Support Contact Us At EnviroLogix 500 Riverside Industrial Parkway Portland ME 04103 1486 USA Tel 207 797 0300 Toll Free 866 408 4597 Fax 207 797 7533 e mail info envirologix com website www envirologix com LIMITED WARRANTY EnviroLogix Inc EnviroLogix warrants the products sold hereunder the Products against defects in materials and workmanship when used in accordance with the applicable instructions for a period not to extend beyond a product s printed expiration date If the Products do not conform to this Limited Warranty and the customer notifies EnviroLogix in writing of such defects during the warranty period including an offer by the customer to return the Products to EnviroLogix for evaluation EnviroLogix will repair or replace at its option any product or part thereof that proves defective in materials or workmanship within the warranty period NEITHER ENVIROLOGIX NOR MONSANTO MAKES ANY OTHER WARRANTIES EXPRESS OR IMPLIED INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILIT
8. r details QuickStix for QuickScan Roundup Ready Corn Bulk Grain Page 3 of 4 Rev 03 21 11 outlined to demonstrate cup size and markings After 30 seconds add QuickStix to cup Strip must develop a Control Line to be valid cut where indicated and read in QuickScan System Kit Storage QuickStix can be stored at room temperature or refrigerated for a longer shelf life Note the shelf life on the kit box for each storage temperature The kit may be used in field applications however prolonged exposure to high temperatures may adversely affect the test results Do not open the desiccated canister until ready to use the test strips Precautions and Notes This kit is designed to be quantitative using the QuickScan System As with all tests it is recommended that results be confirmed by an alternate method when necessary The assay has been optimized to be used with the protocol provided in the kit Deviation from this protocol may invalidate the results of the test The results generated through the proper use of this kit reflect the condition of the working sample directly tested Extrapolation as to the condition of the originating lot from which the working sample was derived should be based on sound sampling procedures and statistical calculations which address random sampling effects non random seed lot sampling effects and assay system uncertainty A negative result obtained when properly testi
9. rip The portion of the strip between the protective tape and the absorbent pad at the top of the strip is used to interpret the reactions as described under Interpreting the Results Results are scanned and interpreted quantitatively with the EnviroLogix QuickScan System Please avoid bending the strips Sample Preparation 1 Collect a composite sample according to USDA GIPSA instructions found in the reference documents listed in the margin on Page 2 2 Determine the average weight of the grain from the lot to be tested Count and weigh 100 kernels seeds then divide by 100 3 Calculate the sub sample weight g needed for testing number of seeds X average seed weight 4 Choose appropriate container based on sub sample weight If using Waring blender container must allow enough free space above the sample for blades to operate 5 Calculate water volume needed for sample preparation The Common Extraction Method calls for a water volume to sample weight ratio of 1 5 to 1 Example Calculation using a 400 kernel sub sample with an average kernel weight of 0 3g 0 3g x 400 120g x 1 5mL 180 mL water for extraction Grind Sub Sample Bunn grinder or equivalent 6 Weigh out subsample based on the average weight per seed calculation 7 Grind subsample using Auto Drip setting if using a Bunn grinder with grinder until all whole grains are broken The sample should be the consistency of coffee
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