MRX Microplate Reader User Manual
Contents
1. Figure 1 Plate Carrier Diagram While the Main menu is displayed the plate carrier can be moved in and out using the up and down A v cursor keys The up key moves the plate carrier in and the down key moves it out Do not place any objects or stand within 150mm 6 5 in of the front of the Reader as this will obstruct the plate carrier MRX Microplate Reader User Manual 5 This page is intentionally left blank MRX Microplate Reader User Manual Chapter 1 Features of the Reader Chapter 1 Features of the Reader The MRX Microplate Reader is a microprocessor controlled photometer It is designed to measure the optical density OD of fluid samples in 96 well microplates in order to determine the level of chemical reactions Figure 2 MRX Microplate Reader A full list of the Reader s technical specifications can be found in Appendix C The Reader provides the following features e Endpoint Kinetic and Agglutination Software e PC compatibility for additional control and data storage e Multi lingual display which guides you through menu choices e Single and dual wavelength reading modes e Selection of up to six filter wavelengths e Data analysis and presentation as OD matrices and or graphically using curve fitting routines e Data output to printer MRX Microplate Reader User Manual 7 Chapter 1 Features of the Reader e Storage of assay test procedures and microplate data on 32. 2 2 Setup Procedures Maintenance Menu Select MAINT from the Setup menu to access the Maintenance menu and view the self test printouts and maintenance reminder settings Self Tests Self tests are performed when the Reader is switched on to make sure everything is working correctly The self test can be printed if necessary so that a record can be kept The Reader will display the current self test print setting Select ENTER to confirm the setting or CE to access the options PRINT SELF TEST 4 45P 30 06 94 YES NO CE Select YES to print the self tests each time they are run Le Note The printer must be connected directly to the Reader for the self test to print An extra self test retest is also available which is carried out before each plate is read The Reader will display the current setting for the test Select ENTER to confirm the current setting or CE to access the options TEST BEFORE READ 4 45P 30 06 94 YES NO CE Select YES to perform the extra test or NO to omit it L Note Adding the extra test will lengthen the reading time Maintenance Reminder The Reader has a maintenance reminder which can be displayed at regular intervals on power up The reminder is intended for use by laboratories which routinely verify the operation of their instruments The Reader will display the current setting Select ENTER to confirm the current setting or CE to access the options MAINTENANCE 4 45P 30 06 94
3. Assay Wizard 59 Running a Plate naceron eege 60 Data Menu recen e e RE Eee ie 61 View EE 61 Utilities Meni EE 61 Temperature Control Option E cchvccccecscctsertiva iepad ahine eee 61 ere 61 Agglutination OPUOt as see 62 TNE EE OO MRX Microplate Reader User Manual iii About This Manual About This Manual This manual has been written for the laboratory technician and describes how to use the MRX Microplate Reader With the information in this manual you can e Install the Reader e Configure the Reader using the Setup menu to suit your particular needs e Connect the Reader to a printer e Perform basic maintenance procedures This manual also describes all the features and specifications of the Reader hardware and software For information on how to use any software you have installed such as Endpoint or Barcode software refer to the individual user manuals For information on how to connect the Reader to an external computer refer to the Endpoint Program User Manual For explanations of error messages and possible solutions refer to the Troubleshooting Guide MRX Microplate Reader User Manual 1 This page is intentionally left blank MRX Microplate Reader User Manual Safety Information Safety Information If there is any doubt or concern about the safety of the instrument contact an approved service center Warning Although this instrument is fully insulated and earthed grounded it is Elec
4. e MAKE SURE that the voltage ratings on the rear panel of the Reader correspond to the local mains supply MRX Microplate Reader User Manual 3 Safety Information e MAKE SURE that the mains power cable is correctly wired Color codes are as follows Brown Live Live Blue Neutral Neutral Green yellow Earth ground Ground Fuses The instrument does not contain any user replaceable internal fuses However the power supply contains an internal fuse in the primary circuit If the fuse needs to be changed it must be replaced with a CSA approved 20x5mm 4A cartridge fuse with a voltage rating of 250V This fuse should not be replaced by the user If the fuse blows contact an approved service center 4 MRX Microplate Reader User Manual Symbols Symbols Warning Symbol For your safety the Reader is marked with a warning symbol to remind you of certain hazards e On the back panel this indicates that the optics door may be hot If you want to remove the optics door make sure that you allow the Reader to cool before touching it e Adjacent to the mains outlet this indicates that the outlet should only be used for an external monitor with a power rating of up to 120VA and an earth leakage current of less than 1 mA at 264V and 60 Hz Plate Carrier Diagram The diagram on the inside of the floppy disk drive door shows the mechanism for moving the plate carrier in and out
5. Printer Select PRINTER to view the printer settings MRX Microplate Reader User Manual 25 2 2 Setup Procedures Page Size Lab Name EPSON LX 810 4 45P 30 06 94 NEXT LAST ENTER CE Select ENTER to accept the printer shown or use the NEXT and LAST commands to scroll through the list of printers The Reader is supplied with a number of standard printer drivers If your printer is not listed here contact the service center The Reader asks you to enter the page size in inches For example PAGE LENGTH 11 000 4 45P 30 06 94 ENTER CE Select ENTER to accept the page length and width shown or press CE to enter new values The Reader asks if you want to print a laboratory name at the top of each print out sheet LAB 4 45P 30 06 94 YZ ABCD ENTER CE Enter a laboratory name or select ENTER to return to the System menu Laboratory names may be up to 16 characters in length and consist of the following characters e Numbers 0 9 and a decimal point Enter these using the keypad e Upper case letters lower case letters and symbols Enter these in the following way a Choose upper or lower case letters or the symbols using the A and v cursor keys a Scroll through the display characters on the display using the lt and gt cursor keys a Enter the character shown by the flashing cursor by pressing the A function key below it When the name is complete select ENTER MRX Microplate Reader User Manual
6. protocol Electrical Light source 75W tungsten halogen lamp Mains input 100 to 120V or 200 to 240V 50 to 60 Hz Power consumption 500 VA Fuses There are no internal user replaceable fuses The power supply contains a CSA approved 20x5mm 4A cartridge fuse with a voltage rating of 250V in the primary circuit If the fuse blows contact the service center Mains Power Fuse 5A U K mains plug powercord Safety Class apparatus MRX Microplate Reader User Manual 51 Appendix C Specifications Physical Environmental Conditions 52 Weight Dimensions Operating Temperature Operating Humidity Storage Temperature Storage Humidity Altitude 12 5 kg 27 5lb D390 x W360 x H220 mm 10 40 C 10 90 RH 0 50 C 10 90 RH lt 2000m MRX Microplate Reader User Manual Appendix D MRX Training Checklist The MRX Training Checklist is provided as a guide to ensure the critical functions of installation operation maintenance and use of software have been reviewed MRX All Types O Reader set up O Serial number O Power outlet on off button communication ports and floppy drive O Lamp filters and software O Printer set up and other peripherals Maintenance O Replace lamp O Change filters O Cleaning Run Verification Plate Review User s Manual Table of Contents Review List of Software Anomalies If Any MRX Microplate Reader User Manual 53 Appendix D MRX Training Checklist MRX
7. DATE 4 45P 30 06 94 ENTER CE Select ENTER to confirm the date shown or type in a new date To edit the date entered select CE use the keypad to enter a new date and then select ENTER MRX Microplate Reader User Manual 2 2 Setup Procedures System Menu Select SYSTEM from the Setup menu to access the System menu and view the printer and user interface settings SETUP 4 45P 30 06 94 LANGUAGE DISPLAY KEYPAD PRINTER CE Language Select LANGUAGE to set the language options The Reader will display the current language setting Select ENTER to confirm this setting or CE to access the options LANGUAGE 4 45P 30 06 94 ENGLISH FRANCAIS DEUTSCH ITALIANO OTHER OTHER accesses a second Language menu with the ESPANOL option Select the required language or select CE to return to the System menu The language chosen will be used to display all subsequent menus Display Select DISPLAY to view the Reader display format DISPLAY 2 X 40 4 45P 30 06 94 ENTER CE Press CE or ENTER to return to the System menu Keypad Select KEYPAD to view the Reader keypad format KEYPAD 21 KEY 4 45P 30 06 94 ENTER CE Select ENTER to move on The keypad has an optional beep which sounds each time a key is pressed The Reader will display the current key beep setting Select ENTER to confirm the setting or select CE to access the options KEY BEEP 4 45P 30 06 94 YES NO CE Select YES to switch the beep on or NO to switch it off
8. a Place the filter with the lowest wavelength in position 1 the next lowest in position 2 and so on a Make sure the side of the filter that has the shorter distance from the groove to the end is toward the lamp a The Filter Blanks must be fitted into the unoccupied filters positions in the Filter Wheel a Make sure the Filter Springs are around the sides of the Filter as shown in Figure 10 The Springs must not extend past the rear face of the Filter as shown in Figure 11 Otherwise the self test will fail due to Filter Motor Errors MRX Microplate Reader User Manual 17 2 1 Installation 18 Figure 10 Correct Filter Spring Positioning view of Filter Wheel from filter spring side Figure 11 Incorrect Filter Spring Positioning Slide the optics assembly back in if you have an older instrument you must also reconnect the 10 way connector and replace the optics door Attach the lamp connector to back of lamp Switching the Reader On Before switching the Reader on note the position of the voltage selector see Figure 4 or Figure 5 The position of the selector is very important and must be adjusted to match the line voltage of the mains power supply Position of Voltage Selector Mains Line Voltage 115V 100 120V 230V 200 240V If the voltage selector is not in the correct position refer to the Voltage Selector discussion on page 14 DO NOT place any objects or stand within 150mm of the front of the Read
9. 5 floppy disk drive or hard disk e Barcode option e Test plate option e Temperature Control option 8 MRX Microplate Reader User Manual Chapter 2 Installation and Setup Chapter 2 Installation and Setup 2 WMS Ee e EE 11 Unpacking the Reader eege ee site sues 11 The MRX77 and MRXpjys Back Panel NEEN 12 The MRX Revelation Back Panels EE 13 reene EE 14 The MRX Front Panelni ssc ce ent ee 15 The Keypad and Display EE 15 Windows QWERTY Keyboard Option 16 Fitting the Lamp and Optical Filters 20 0 0 cece ecesseceenceceeneeceenneeeeeaes 17 Switching the Reader EE 18 Switching between DOS Applications cc eeeceeeseeceeneceenteeeeneeees 20 22 Setup EE eege 21 SIE Mentana aoee a a E E AE E a a 22 Filters ME rer ee ee eener 23 Clock E EE 24 System Ment eege Ee EE Seen 25 M int nance Menmen ee ee 27 Comms Ment tee ee eerie ison A e SE ASS RRASA 28 Memory Men Geteste een eer ee E En aaa 29 Param RE EE 30 MRX Microplate Reader User Manual 9 Chapter 2 Installation and Setup This page is intentionally left blank 10 MRX Microplate Reader User Manual 2 1 Installation 2 1 Installation CAUTION DO NOT connect the instrument to the mains electrical supply or switch it on before you have completed this installation procedure To review the critical functions of installation operation maintenance and software use refer to the MRX Training Checklist in Appendix D Unpacking the Reader If you ordered an upg
10. 54 Power On Self Diagnostic Test O Print self test Proper Care of Disks and Backup of Assays and Data Stored on Disks Endpoint Software Set Up Filters Printer check setting anytime printer goes off line Clock MRX parameters Maintenance schedule m0000 0 0 Communication parameters interface with external computer see next section Creating a New Test O Test numbers names and plate ID O Shaking O Defining a template Well types B S T C user definable gt Replicates gt Fill row column Change O Wavelength mode single dual multiple O Blanking options O Optical Density OD Data Matrix MRX Microplate Reader User Manual Appendix D MRX Training Checklist O Data reduction gt Curve Fitting gt Thresholds gt Ratios gt Differences gt QC equations gt Area statistics O Password protection Editing a Test Running a Test and Recalling Data Printing Data Tests Directories Utilities O Manual control of the reader O Long and short menus O Version O Statistical data gt Coefficient of Variation gt Statistical data for stored plates O Batch O Spectral Response Curve Temperature Control O Safety information O Features O Software installation O Setting the temperature MRX Microplate Reader User Manual 55 Appendix D MRX Training Checklist 56 Kinetics Software O Features values determined from a reaction curve O Calculation mode
11. Figure 5 Plug the monitor cable into the Monitor Port labeled 8 on Figure 5 Plug the mouse cable into the PS 2 Mouse Socket labeled 5 on Figure 5 If you are also running DOS programs they will not run correctly while the mouse is plugged in Always unplug the mouse before running a DOS program Only use the monitor keyboard mouse and software supplied with the MRXpRevelation Do not install any unauthorized hardware or software as this may affect the reliability of the Reader and will invalidate the warranty 16 MRX Microplate Reader User Manual Fitting the Lamp and Optical Filters Warning Disconnect the mains cable before fitting lamp and filters Note DO NOT touch the filters bulb or reflector directly 1 Using a No 1 Posi driver remove the optics door and if fitted remove the packing materials from behind the door 1 Slide the optics assembly out of the instrument If you have an older instrument check your serial number panel newer Readers have 1CXCxxxx or 2CXCxxxx numbers you need to disconnect the optics assembly s 10 way connector and then slide the assembly out of the instrument 1 Insert the lamp into the assembly and plug the connector into its back see Figure 9 Make sure that the lamp is seated correctly with the locating pip on the lamp fitted into the notch on the optics assembly Figure 9 Fitting the Lamp and Filters 4 Insert the filters into the filter wheel
12. ME E EE 11 Unpacking the Reader tee 11 The MRX z and MRXpjys Back Panel un 12 The MRX Revelation Back Panel sissy nese cnsds cancasedsndeeviseneptumteeesecderenctbesieas 13 e 14 The MRX Front Panel sts ee 15 The Keypad and Display senene ainese nE a 15 Windows QWERTY Keyboard Option eccceeeececeeseceeneeeenteeeees 16 Fitting the Lamp and Optical Filters 2 0 0 0 ceeeeeeeececeenceceeneeceeeeeeeees 17 Switching the Reader EE dasscay aeaceaweeeenneeeneeee 18 Switching between DOS Applications 0 0 0 0 cecceeeecceceeeeceeneceenteeeenaes 20 DD SOUP Te 21 Setup VICI aes er tacts ccna lceang tevin nage eecncactsy E tase AE aneet 22 Filters Ee neee AE A E REAS 23 Clock MISTI ks eege 24 System EE EE 25 Maintenance Ehe 27 Comms Mene aeo ares aa EE AS E E a aaa tonne 28 Memory Mii TEE 29 Param let 30 Chapter 3 Printer Interface ssecessoesssoosessocesssoeessoossssose 33 Chapter 4 Maintenance eessssececssssocecssssececssssececessssccessoo 35 4 1 Routine Maintenance Drocedures 37 EN e rE E E E ont nes EO EO EATS 38 PROC LAN TIS ohsem aa tee E E E A AO ET ege 38 MRX Microplate Reader User Manual i External Painted and Plastic Surfaces 0 ceescceeeeeeeeeeneeeeeneeeeeeeeeenes 38 Ee 38 Re ee susie ard ae eae 38 4 3 Repacking eelere eeee eegene 39 4 4 Replacing the EE 40 4 5 Changing the Optical Filters cs stv cucetsscahenes anes Oeovertucaeecohernies 41 Appendix A Self Test Diagnostics ccsss
13. NO DAILY WEEKLY MONTHLY CE Select NO if you do not want any reminders to be displayed or select the appropriate frequency MRX Microplate Reader User Manual 27 2 2 Setup Procedures Comms Menu Select COMMS from the Setup menu to access the Communications menu and view PC communications settings Note Do not alter the default settings unless you are proficient in RS232 communications protocols SETUP 4 45P 30 06 94 MR700 MRX USER CE MR700 Select MR700 to set the Reader communication parameters to a format suitable for all DYNEX Technologies application programs The MR700 settings are Baud Rate 9600 Stop Bits 1 Data Bits 8 Parity even Data Format MR700 Communications The Reader asks which communication port is being used Select COM Port oe COM2 to specify the port or select CE to return to the Comms menu MRX Select MRX to set the Reader communication parameters to an enhanced format suitable for most application programs When MRX is selected the display flashes briefly and then redisplays the setup menu The MRX settings are Baud Rate 9600 Stop Bits 1 Data Bits 8 Parity even Data Format MRX Communications The Reader asks which communication port is being used Select COM1 Ports or COM2 to specify the port or select CE to return to the Comms menu Note When using a MRX no COM2 option exists MRX Microplate Reader User Manual 2 2 Setup Procedures User Select USER to customize the
14. clean dry amd fit for transpari Signed lie Drite WEN Teckoecboates reserves the nghi bo refus improperiy cleaned egaipasermi Shopping Address DYNMEX Technologma Abin Abore reium mambkeri 4340 Sullyfield Circle Chantilly NA 2151 1683 Suerested decontamination methods Brice Wash all surfaces with a MA bleach solution Follow ihai with a mild deterpen solaio Washers Please follow lke Decanlamoinalbon Procedur found in the back af the MRX Microplate Reader User Manual 47 Appendix B Accessories This page is intentionally left blank 48 MRX Microplate Reader User Manual Appendix C Specifications Appendix C Specifications Software Specifications Menu language English French German lItalian Spanish Clock function Day date and time MR Standard Endpoint Program Optional Kinetic Program and Agglutination Program MRX plus Standard Endpoint Program Kinetic Program and Agglutination Program MRX revelation Standard Revelation Program Program Load Using MX lt 40 seconds mime Using MRXpys lt 1 Second Hardware Specifications MS DOS Version Keypad 21 key tactile membrane Display 2 line by 40 character LCD No of filters 2 6 Windows Option Keypad QWERTY Display Super VGA Color No of filters 2 6 Performance Read cycle time Single Wavelength lt 4 seconds Dual Wavelength lt 6 seconds MRX Microplate Reader User Manual 49 405 to 850nm Wavelength Range Dynami
15. mode Threshold matrix Recalculating results Read mode Tissue Culture Growth TCG Plate speed Readings per well Defining the template Blanking Results matrix Transmission graphs Threshold matrix O Editing a test MRX Microplate Reader User Manual 57 Appendix D MRX Training Checklist O Running a test and recalling data O Printing data tests directories MRX jus or MRX from an External Computer Using Revelation Software Self Tests O Print the self test The Utility Menu O Configure reader Internal MRX External MRX gt Set up MRX gt Filters O Spectral Response Curve O Sample ID entry O Options gt Quick assay buttons Preferences Automatic data save Auto print Plate ID Laboratory information Directories O System password 58 MRX Microplate Reader User Manual Appendix D MRX Training Checklist Pull Down Menus Tool Bar Short Cut Keys Assay Buttons O New assay Endpoint gt Kinetics O Open various types of files Assays data sample IDs text files curves O Importing files Creating an Assay Assay Wizard O Title password bar code O Reader control gt Read modes Single Dual Multi Wavelength Agglutination gt Shake modes Start Stop modes gt Kinetics Time interval Calculation mode OD Min modes Time modes Moving Average Cut Off Graphs O Defining a template gt Well types B S T C user definable gt Replicates gt Fill row co
16. summarizes the lamp replacement instructions Note DO NOT touch the bulb or reflector directly 1 Switch off the mains power supply and disconnect the cable 1 Using a No 1 Posi driver remove the optics door 1 Slide the optics assembly out of the instrument If you have an older instrument check your serial number panel newer Readers have 1CXCxxxx or 2CXCxxxx numbers you need to disconnect the optics assembly s 10 way connector and then slide the assembly out of the instrument 1 Detach the lamp from the assembly and replace with a new one Make sure that the lamp is seated correctly refer to Fitting the Lamp and Optical Filters on page 17 1 Slide the optics assembly back in if you have an older instrument you must also reconnect the 10 way connector 1 Replace the optics door and reconnect the power supply Figure 14 Replacing the Lamp 40 MRX Microplate Reader User Manual 4 5 Changing the Optical Filters 4 5 Changing the Optical Filters Warning If the Reader has been used recently the optics door and the lamp in the optics assembly may still be hot Allow time for the Reader to cool before touching it Take care when removing the optics door as there may be broken glass in the optics assembly Figure 15 summarizes these instructions Note DO NOT touch the filters bulb or reflector directly 1 Switch off the power supply and disconnect the mains cable 1 Using a No 1 Posi dr
17. test results see Appendix A for a sample or save them to a file e Back up the data files for a MRX7 remove the disk and back it up using the File Manager program on an external PC On a yearly basis e Remove the filters and clean them Section 4 2 contains more detailed cleaning instructions Periodic maintenance should be determined by the user MRX Microplate Reader User Manual 37 4 2 Cleaning 4 2 Cleaning Warning Always switch the instruments off and disconnect the power cable before cleaning the Reader The Reader is constructed from high quality materials nevertheless spilt saline solutions solvents acids or alkaline solutions must be removed from outer surfaces immediately to prevent damage Autoclaving DO NOT autoclave any part of this instrument External Painted and Plastic Surfaces Clean external surfaces with a mild laboratory detergent followed by 10 bleach or 70 alcohol Always dilute detergents according to the manufacturer s instructions Filters Clean the filters by wiping them with a lint free cloth or a lens tissue If necessary a little alcohol may be used with the cloth or tissue Decontamination If you need to return the Reader to the service center and it has been in contact with human blood other potentially infectious body fluids pathological samples toxic or radioactive materials it must be decontaminated Before shipping the instrument do the following e Clean the
18. 0 MRX Microplate Reader User Manual 2 2 Setup Procedures 2 2 Setup Procedures The Reader has a number of settings which can be adjusted to suit the assays which will be run on it The settings are altered using the Setup menu To use the Setup menu options select SETUP from the Main menu The options accessed by this menu are summarized in the table below and described in detail in this section p 23 Filters kode amin Pei German Spanish 0 25 Printer various Dei PARAM p 30 Plate Type 12x8 10x4 Repeatability 1 4 readings L Note If you are using a MRXj Reader and you make changes to the Setup settings these changes will only apply to the current software program If you insert a different program disk you will have to re enter the settings MRX Microplate Reader User Manual 21 2 2 Setup Procedures Setup Menu Example The Setup menu allows you to view and if necessary alter the Reader settings SETUP 4 45P 30 06 94 OTHER FILTERS CLOCK SYSTEM CE Select OTHER to access a second Setup menu SETUP 4 45P 30 06 94 MAINT COMMS MEMORY PARAM CE In the menus shown on the following pages the options available are shown on the bottom line of the display To select an option press the function key below it or where appropriate key in a value Note In most of the menus the default or previously defined value is shown To enter a different value you must press CE This will access the alterna
19. DYNE X Absorbance 3 Plate Washing E User e Manual Ss for MRX Software DYNEX Technologies Inc 14340 Sullyfield Circle Chantilly VA 20151 1683 Phone 703 631 7800 FAX 703 803 1441 www dynextechnologies com This page is intentionally left blank rts MRX Microplate Reader User Manual IMPORTANT Please read carefully before using the MRX Microplate Reader Rev 11 04 02 Part No 99001840 Software Version Instrument N This document is the copyright of DYNEX Technologies Inc and must not be copied or reproduced in any form without prior consent DYNEX Technologies reserves the right to make technical improvements to this equipment and documentation without prior notice as part of a continuous program of product development This manual supersedes all previous editions MS DOS Licensing This product contains software licensed from Microsoft Corporation 1 The MRX contains intellectual property that is software programs that are licensed for use by the end user customer hereinafter referred to as the end user This is not a sale of such intellectual property The end user shall not copy disassemble or reverse compile the software program The software programs are provided to the end user as is without warranty of any kind either expressed or implied including but not limited to warranties of merchantability and fitness for a particular pur
20. Endpoint gt Kinetics Delta Total total rate of change in OD Min Best greatest rate of change in OD Min Linear average rate of change in OD Min gt Kinetics Time OD time to a certain OD OD Min time to a certain rate of reaction Relative time to an OD relative to the first OD taken Kinetics single well monitoring gt Options Increasing or decreasing OD Time intervals No of readings Scaling Results Log Display a well Creating a New Test Test numbers names and plate ID Shaking Defining a template Wavelength mode single dual multiple Blanking options Start mode Immediate OD OD Min Time Data reduction Curve Fitting Thresholds Ratios Differences QC equations Area Statistics MRX Microplate Reader User Manual Appendix D MRX Training Checklist O Editing a test and ODs O Running a test and recalling data gt Kinetic output All matrix of plots of 96 Wells Zoom plot of single well Row and column plots O Printing data tests directories Agglutination Software O Agglutination mode gt Principles gt Interpreting results Calculation modes Formula and Rules O Tissue Culture Growth TCG mode Principles Interpreting results O Creating a new test Test numbers names and plate ID gt Shaking gt Filter selection gt Read mode Agglutination Noise filtering Transmission graphs Defining a template Data Log Calculation
21. age of the mains power supply before switching the Reader on Position of Voltage Selector Mains Line Voltage 115V 100 120V 230V 200 240V FAILURE TO FOLLOW THE PROCEDURES DETAILED BELOW MAY RESULT IN DAMAGE TO THE MICROPLATE READER DUE TO PARTS FALLING INTO THE POWER SUPPLY There are two types of plastic cover fitted to the voltage selector switch e If the plastic cover has a slot cut in it and the voltage selector is not in the correct position insert a suitable small flat bladed screwdriver through the slot and move the selector to the desired voltage DO NOT LOOSEN THE SCREWS USED TO RETAIN THE PLASTIC COVER e If the plastic cover does not have a slot cut in it and the voltage selector is not in the correct position use a No 1 Posi driver to remove one of the screws and loosen the other screw to allow the plastic cover to be pushed aside Move the switch to the desired voltage replace the cover and tighten the screws DO NOT REMOVE BOTH SCREWS USED TO RETAIN THE PLASTIC COVER MRX Microplate Reader User Manual 2 1 Installation The MRX Front Panel ZS Jamaal am L Biz O POWER dies EL Figure 6 The MRX Front Panel 1 Power Switch marked I O for 4 Floppy Disk Drive on off 2 Power Light 5 Keypad and display 3 Plate Carrier Doo
22. c range 0 100 to 4 000 OD Linearity 0 000 to 2 000 OD 1 0 2 001 to 3 500 OD 1 5 Filter band width FWHM 10nm 2nm Center Wavelength Accuracy 2nm Precision 0 000 to 2 000 OD 0 2 CV or 0 005 OD whichever is greater 2 001 to 3 000 OD 0 6 CV 3 001 to 3 500 OD 1 0 CV Accuracy 0 000 to 3 500 OD 2 5 or 0 005 OD whichever is greater 340 to 400 Wavelength Range Option Dynamic range 0 100 to 3 000 OD Linearity 0 000 to 2 500 OD 2 0 Filter band width FWHM 18nm 2 nm Center Wavelength Accuracy 2nm Precision 0 000 to 2 000 OD 0 6 CV or 0 005 OD whichever is greater 2 001 to 2 500 OD 1 0 CV Linearity is the maximum percentage deviation of a reading from the ideal straight line plot of OD versus concentration Measured by the manufacturer at 410nm in the visible range and 340nm in the UV range Precision is the mean Coefficient of Variation CV figure for all wells on a plate that has been read 20 times in succession Measured by the manufacturer at 410nm in the visible range and 340nm in the UV range 50 MRX Microplate Reader User Manual Appendix C Specifications Interface Printer Interface Parallel Centronics Compatible with up to 850 printers All printers must be compliant with EMC directive 89 336 A number of standard printer drivers are included If your printer is not one of these contact the service center Computer Interface Bi directional RS232C with user definable
23. ccccssssssssccsseees 43 Self Lest Sampl EE 44 Appendix B ACCeSSOriesS esssecccessseocceessoocccesssooccesssoseesesssse 45 Appendix C Specifications eesssecccsesssocccsessocsccsesssoososessosse 49 Kate EE 49 Hardware Specifications 42s snaiseccec bt eaecaceesnenetss ine aea AO EEEa 49 MS DOS VEETSIN EEN 49 Windows OPON E 49 e ee 49 405 to 850nm Wavelength Range 00 0 eeececeseceesseeeceeececeeaeeceeneeeeeaes 50 340 to 400 Wavelength Range Chppon 50 lat e 51 5 ore ta NET 51 Physical Environmental Conditions c ccessccessseceeeeeeeseeneeceeneceeeaees 52 Appendix D MRX Training Checklist ssccssssooes 53 MRX AI EE 53 EE 53 R n Verification EE 53 Review User s Manual Table of Contents 0 0 0 0 ee eeeececeeeeeeeenteeeeees 53 Review List of Software Anomalies If An 53 MRX ege eege 54 Power On Self Diagnostic Test 2 c2 i2ccquieciesceseauceetnsactectaneeqrncetees 54 Proper Care of Disks and Backup of Assays and Data Stored on Disks54 ele eer 54 LE e ut 55 Temperature Control eege 55 Kinetics SOR EE 56 Agglutination Software sseeeseseeseessesesseressersseesssresseesseeesssresseeessrees 57 MRX plus or MRX77 from an External Computer Using Revelation SEET 58 SEI ESOS e eae ania ree and eo Ree 58 PRS Ay Ment soroaren R A 58 Pull Down Menus Tool Bar Short Cut Keys Assay Buttons 59 MRX Microplate Reader User Manual Table of Contents Creating an Assay
24. communication settings The User options allow you to set the values for baud rate stop bits data bits parity and data format The settings available are Baud Rate 110 150 300 600 1200 24 00 4800 9600 Stop Bits 1 2 Data Bits 7 8 Parity none even odd Data Format MR700 extended MRX Memory Menu Reset Select MEMORY from the Setup menu to access the Memory menu which allows you to reset the Reader memory or to upgrade the software SETUP MEMORY 4 45P 30 06 94 RESET UPGRADE CE To upgrade the Reader follow the instructions provided with the upgrade disk The RESET command resets the Reader s default settings overwriting all user defined setup parameters The Reader will ask you to confirm this action ARE YOU SURE 4 45P 30 06 94 YES NO CE Select YES to reset all the Reader s parameters or NO to return to the Memory menu Upgrade The UPGRADE option allows you to upgrade the Reader with a software upgrade disk Only Reader application software obtained from your supplier should be used with this command If you are upgrading the Main menu Setup software refer to Upgrading From a Previous Version in Chapter 3 in the Endpoint Program User Manual MRX Microplate Reader User Manual 29 2 2 Setup Procedures 30 Param Menu Select PARAM from the Setup menu to access the Parameters menu and view the OD limit data conversion plate type and repeatability settings Over Limit Le Data Conve
25. display Filter 1 and a wavelength for example FILTER 1 410 nm 4 45P 30 06 94 ENTER CE Select ENTER to confirm the wavelength shown and move on to Filter 2 Select CE to clear the wavelength shown and enter a new wavelength Press ENTER When wavelengths have been entered for all the filters installed the display will return to the Setup menu MRX Microplate Reader User Manual 23 2 2 Setup Procedures Clock Menu Time Date Select CLOCK from the Setup menu to access the Clock menu and view the time and date settings SETUP 4 45P 30 06 94 TIME DATE CE Select TIME to set the clock mode and time The Reader will display the current setting Select ENTER to confirm the mode shown or select CE to display the alternatives CLOCK MODE 4 45P 30 06 94 12 HOUR 24 HOUR CE Select 12 HOUR or 24 HOUR mode The Reader will allow you to change the time display TIME 4 45P 30 06 94 ENTER CE Select ENTER to confirm the time shown or enter the correct time using the keypad e If you selected the 24 hour mode enter the time and press ENTER e If you selected the 12 hour mode enter the time and then select A M or P M Select DATE to display the date mode The Reader will display the current setting Select ENTER twice to confirm the mode shown or select CE to display the alternatives DATE MODE 4 45P 30 06 94 dd mm yy mm dd yy CE Select dd mm yy or mm dd yy The Reader will allow you to change the date display
26. er as this will obstruct the plate carrier MRX Microplate Reader User Manual 2 1 Installation 1 Make sure that the instrument is switched off The power switch is ON when it is flush with the front panel and OFF when it stands out from the panel 2 For a MRX insert a Reader program disk into the floppy disk drive 3 Insert the power cord supplied into the socket at the rear of the instrument 4 Connect the power cord to a suitable power source with a protective earth ground Connect the printer cable from the Reader to the printer Switch the printer on Switch the Reader on by pressing the power switch on the front panel O N OA Verify that the Reader completes the self test routine successfully Self Tests When the Reader is switched on it carries out several self tests to check that the instrument is working correctly Appendix A shows a list of these tests and a sample printout During the self tests a series of symbols are displayed on the bottom line of the LCD these indicate the optional extras that are installed in your Reader If the Reader fails any of the tests an error message is displayed If the Reader s data files have been corrupted the Reader requests your serial number this can be found on the Reader s back panel or inside of the floppy disk drive s door If an error message persists contact the service center Maintenance Reminder On first power up the Reader may displa
27. erate successfully with the Reader Le Note Once the printer has been correctly installed it should always be switched on before switching on the Reader An external printer can be connected to the Reader using the interface cable shipped with the instrument This is a ribbon cable terminated by a standard IEEE Centronics style 36 way connector see Figure 12 Figure 12 Printer Cable Connector Centronics The table below shows the relevant pin assignments Pin No Signal Name 1 STROBE 2 DATA 1 3 DATA 2 4 DATA 3 5 DATA 4 6 DATA 5 7 DATA 6 8 DATA 7 9 DATA 8 MRX Microplate Reader User Manual Pin No 19 20 21 22 23 24 25 26 27 Signal Name GROUND GROUND GROUND GROUND GROUND GROUND GROUND GROUND GROUND 33 Chapter 3 Printer Interface 34 10 11 12 13 14 15 16 17 18 ACKNLG BUSY PE SLCT Not Used Not Used Not Used Not Used Not Used 28 29 30 31 32 33 34 35 36 GROUND GROUND GROUND INIT Not Used Not Used Not Used Not Used Not Used MRX Microplate Reader User Manual Chapter 4 Maintenance 4 1 Routine Maintenance Drocedures 37 EE eenegen 38 E E 38 External Painted and Plastic Surfaces ee SEENEN 38 Filer E E N 38 Decontam
28. g Removing readings Filtering noise Calculating results as a percentage of a specific well MRX Microplate Reader User Manual Microplate Reader User Manual 105202A A A Accessories 45 B Back panel MRX and MRX ius 12 Back Panel MRX revelation 13 C Cleaning 38 Clock Menu 24 25 Comms Menu 28 29 D Decontamination 38 Display 15 F Electric Shock Hazard 3 Equipment in Transit form decontamination See Appendix B External Painted and Plastic Surfaces cleaning the 38 F filter spring positioning 18 Filters cleaning the 38 Filters Menu 23 Front panel 15 Function keys 15 MRX Microplate Reader User Manual Fuses 4 H Hardware Specifications 49 SS oe Incorrect Operation 3 _K Keypad 15 D Lamp fitting the 17 18 17 18 replacing the 40 M Main Menu 19 Maintenance 37 38 Maintenance Menu 27 Maintenance Reminder 19 27 Memory Menu 29 Microplate Reader See Reader MRX training checklist 53 MRX front panel 15 MRX and MRX ius back panel 12 MRX krevelation back panel 13 0 Optical filters changing the 41 42 Optical Filters fitting the 17 18 63 64 Over Limit 30 Param Menu 30 31 Plate Carrier Diagram 5 Plate Type 30 Printer interface 33 34 R Reader accessories 45 back panel MRX and MEN 12 back panel MR revelation 13 connecting a prin
29. ination eeir ae E E E ee 38 4 3 Repacking E 39 4 4 Replacing the Lamps sss savendariatieasio iain oe ae 40 4 5 Changing the Optical Filters s ccciscpsnaviowteaiatieweaevasnenns 41 MRX Microplate Reader User Manual 35 Chapter 4 Maintenance This page is intentionally left blank 36 MRX Microplate Reader User Manual 4 1 Routine Maintenance Procedures IMPORTANT The first section in this manual contains safety information for all the equipment in the system You should be familiar with this information anyway but if not you must read through this section before carrying out any maintenance The warranty on the Reader may become void if you do not follow the safety and maintenance instructions contained in this manual or if the covers are removed by anyone other than approved service personnel 4 1 Routine Maintenance Procedures The Reader requires very little maintenance however certain simple procedures should be carried out on a regular basis On a daily basis e Verify self test passes e Remove the microplate from the plate carrier wipe the plate carrier down and return it to the In position e Wipe down the external surfaces Section 4 2 contains more detailed cleaning instructions On a weekly basis e Review the results of the self test that occurs when you switch the Reader on to make sure the instrument is functioning correctly If you want to retain this information for your records print out the self
30. iver remove the optics door 1 Slide the optics assembly out of the instrument If you have an older instrument check your serial number panel newer Readers have I1CXCxxxx or 2CXCxxxx numbers you need to disconnect the optics assembly s 10 way connector and then slide the assembly out of the instrument 1 Remove the relevant filter from its socket in the filter wheel and replace with the new one e Make sure the side of the filter that has the shorter distance from the groove to the end is toward the lamp e Make sure the Filter Springs are around the sides of the Filter as shown in Figure 10 on page 18 The Springs must not extend past the rear face of the Filter as shown in Figure 11 on page 18 Otherwise the self test will fail due to Filter Motor Errors 1 Slide the optics assembly back in if you have an older instrument you must also reconnect the 10 way connector 1 Replace the optics door and reconnect the power supply GE cs Figure 15 Changing the Optical Filters Le Note If you install new filters you should run the test plate software Be certain to run PARAMS to establish new baselines MRX Microplate Reader User Manual 41 4 5 Changing the Optical Filters This page is intentionally left blank 42 MRX Microplate Reader User Manual Appendix A Self Test Diagnostics Appendix A Self Test Diagnostics On power up and optionally before every read the Reader performs several self tes
31. lumn random gt Change template O0 Blanking options O QC equations for raw data MRX Microplate Reader User Manual 59 Appendix D MRX Training Checklist O Data reduction gt Curve Fitting Standards Fit types and axes Combine on same graph Use stored fit Calculated QC Data conversion Output format Thresholds Threshold equations Segments Labels QC Output format gt Ratios Spreadsheet O Report options gt Report layout Data Matrix gt Text file Options Open Microsoft Excel and import text file O Assay options gt Area statistics Sample IDs gt Processing order Running a Plate O Auto save print search for sample IDs Runa partial plate even though all wells are defined by well type O Adding assays to the check list Multiple assays on a plate 60 MRX Microplate Reader User Manual Appendix D MRX Training Checklist Data Menu O Reprocessing data Data refresh gt Select new assay Variable parameters Reagent lot number Outlier removal Titration multiplier Fit information Kinetic graphs D DDDD DD Sample IDs View Menu Utilities Menu O Plate Plate statistics Temperature Control Option O Features O Setting O Control Log Barcode Option O Types O Features MRX Microplate Reader User Manual 61 Appendix D MRX Training Checklist 62 Agglutination Option O UD DUDU D Plate speed Calculation modes Transmission graphs Data Lo
32. mbols earlier in this manual 2 The positions of ports 5 and 6 may not be exactly as shown but the number of pins or pin sockets in each port will remain the same 3 Remove the port protectors connector protector and styrofoam packing inside the Optics Door 7 MRX Microplate Reader User Manual The MRX revelation Back Panel Figure 5 The MRX fevelation Back Panel 1 Mains Inlet 8 Monitor Port 2 Voltage Selector see page 14 9 Optics Door 3 Monitor Mains Socket 10 Optics Cooling Vents 4 Keyboard Socket 11 Serial Number Plate 23 PS 2 Mouse Socket 12 PSU Cooling Vents 6 Serial Port 13 Main Enclosure 7 Printer Port L Notes 1 The warning symbols on the back panel indicate that the optics door may be hot and that no appliances other than a monitor should be plugged into the mains outlet For more details refer to the discussion on Symbols earlier in this manual 2 The positions of ports 5 through 8 may not be exactly as shown but the number of pins or pin sockets in each port will remain the same 3 Remove the port protectors connector protector and styrofoam packing inside the Optics Door 7 MRX Microplate Reader User Manual 13 2 1 Installation Voltage Selector CAUTION The position of the voltage selector see Figure 4 or Figure 5 is very important and must be adjusted to match the line volt
33. ories Appendix B Accessories Part Number Filter 630nm 6329780630 Lamp UV shipped standard with 549000600 single board unit Dust Cover MRX and MRXpiys 9119780012 Dust Cover MRX Revelation 9119780011 Lens Cleaning Cloth 41000070 Service Manual single board 99000500 RS 232 Filter 9 way D Connector 50400380 Test Plate 12 way non UV 450nm__ 0119780004 with Manual Insert and Sofware Test Plate 12 way UV with 0119780001 Manual Insert and Sofware Test Plate Software for use with 04000100 upgrade for MRXpixs ingle board unit onl snp Poa deny 04000110 system for MES Ai US Power Cord 6090405009 MRX Microplate Reader User Manual 45 Appendix B Accessories MRX User Manual 17000010 MRX pius User Manual 17000020 MRX Revelation User Manual 17000030 46 MRX Microplate Reader User Manual Appendix B Accessories DYNEX EQUIPMENT IN TRANSIT IMPOR IAY S Please ach a copy ol thas fon wilh cach stamen LE your nsinmament contains a kard drive pense retain back up Ce pies of DI shored Files Failure Io d so may result in the loss of those files Retum Authorization Number Contac Technical Service DYNES Technologies Fonc MISHI i EI fox TOO OE TRIG Equipment Seral Number EQUIPMENT DECLARATION Clearly indicate Faul condition or reason for retum CERTIFICATE OF DECONTAMINATION I certify ihmi dhe equi pment described above has been disinfected decontaminated and is
34. pose The entire risk of the quality and performance of the software program is with the end user D DYNEX Technologies and its suppliers shall not be held to any liability for any damages suffered or incurred by the end user including but not limited to general special consequential or incidental damages including damages for loss of business profits business interruption loss of business information and the like arising from or in connection with the delivery use or performance of the software program MRX and MRXplu h are registered trademarks of DYNEX Technologies MRX uf Assay Wizard and Revelation are trademarks of DYNEX Technologies MS DOS Microsoft Excel and Windows are registered trademarks of Microsoft Corporation l e IBM is a registered trademark of International Business Machines Corporation Apple and Macintosh are trademarks of Apple Computer Inc Copyright 1997 DYNEX Technologies All rights reserved Limitations of Warranty Relating to Software 1 DYNEX Technologies accepts no liability for the maintenance or support of Microsoft software 2 DYNEX Technologies accepts no liability for any consequential loss arising from the use of software on this product outside the scope of the above definition Limitations of Use The user of the MRX must follow the specific manufacturer s assay package insert when modifying parameters and establishing result
35. r The Keypad and Display The Reader is controlled by menu driven software which responds to the user s menu choices The menus are displayed on a liquid crystal display which can show up to five options at a time Choices are entered using the keypad VERSION 1 0 SEEPSTES tien ie CO OCH OC OO OCH Oo A A A A Figure 7 Function Keys and Display e Function keys menu options are shown on the bottom line of the display and selected by pressing the function key below MRX Microplate Reader User Manual 15 2 1 Installation 1 4 25 Sc 40 SO Se 6r 7c 8x CJL JLo Ese Figure 8 Alphanumeric Keys and Cursor Keys e Alphanumeric keys used to enter numbers and well locations e Esc key returns the display to the Main menu e Cursor keys used to scroll through well locations and menus and in some menus to move the plate carrier in and out Le Note When the Reader is operating under the control of an external computer the keypad will not operate Windows QWERTY Keyboard Option If you have bought the MRXpreyelation you Will have to install the monitor keyboard and mouse Keyboard 1 Plug the keyboard cable into the Keyboard Socket labeled 4 on Figure 5 Place the protective cover over the keys Monitor Place the monitor on top of the Reader Plug the power cord into the Monitor Mains Socket labeled 3 on
36. rade you will only receive an upgrade disk and a manual The Reader is packed to provide maximum protection during shipment Take care when unpacking it and examine it carefully for any damage Report any damage to the carrier immediately Check the contents against the shipping checklist enclosed and report any omissions to your supplier Remove the Reader from the carton and place it on a level surface free of dust moisture vibration draughts and away from direct sunlight Keep the packing materials so that they can be used if the Reader has to be transported Figure 3 Packing Contents Example 1 Reader 5 Software disks 2 Manual Set 6 Power Cord 3 Optical Filters 7 Printer Cable 4 Lamp 8 Interface Cable 9 RS 232 Filter not shown MRX Microplate Reader User Manual 11 2 1 Installation The MRX and MRXpus Back Panel Figure 4 MRX and MRXp us Back Panel 1 Mains Inlet 7 2 Voltage Selector see page 14 8 3 Monitor Mains Socket 9 4 Keyboard Socket 10 5 Serial Port 11 6 Printer Port L Optics Door Optics Cooling Vents Serial Number Plate PSU Cooling Vents Main Enclosure Notes 1 The warning symbols on the back panel indicate that the optics door may be hot and that no appliances other than a monitor should be plugged into the mains outlet For more details refer to the discussion on Sy
37. rsion The Over limit is used to set a range within which ODs should fall ODs above the limit will not be processed The limit is also used as an Under limit to set the negative limit of the range For example If 3 500 is set as the over limit ODs above 3 5 will not be stored and will be printed as OVER ODs below 3 5 will be printed as UNDER The Reader will display the current Over Limit Select ENTER to confirm the value shown and move on to the Param Plate Type menu Select CE to clear the value shown and enter a new value Minimum 0 000 Maximum 4 000 Note Take care when setting the Over limit if it is too low valid test results may be rejected The normal limit is 3 500 If the Over limit is altered the Reader asks if ODs which lie outside the range should be assigned a conversion value for use in quality control equations If no value is assigned to these ODs the QC equations which contain them will fail DATA CONVERSION 4 45P 30 06 94 YES NO ENTER CE Select YES to enter a value for Over and Under ODs or select NO to define the plate type The Reader will ask for the Over value followed by the Under value For example OVER VALUE 9 999 4 45P 30 06 94 ENTER CE Enter Over and Under values and select ENTER Min Over value 0 000 Max Over value 9 999 Min Under value 9 999 Max Under value 0 000 Plate Type Two types of plate format can be used with the Reader One is the standard 12 rows b
38. s calculation methods The performance characteristics of the MRX have not been established with specific serology or antigen detection assays The user must evaluate the MRX in conjunction with the specific serology and or antigen detection assays This evaluation must include the establishment of performance characteristics for the specific assays Under no circumstances does the MRX have any direct patient contact or perform any therapeutic patient function Specific diagnostic results are not provided by the device Diagnostic decisions are made only after independent confirmation by additional methods under the supervision of a qualified professional WARNING If this equipment is used in a manner not specified by the manufacturer then the protection provided by the equipment may be impaired Before using any decontamination method except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment This page is intentionally left blank Table of Contents Table of Contents About This Manual sss scccccsccccsssscsecescacesscdscedasesssene doseswevanessaves ese 1 Safety Information secccsssssocccssssecccessssoccesessocecessssccecssssessees 3 kA EE 5 War SVG EE 5 Plate Carrier Did Sarit eer 5 Chapter 1 Features of the Reader sccccssssssscccssssssscees 7 Chapter 2 Installation and Setup cccccsssssscsssseeesssees 9 Aah
39. surface with a mild laboratory detergent followed by 10 bleach e Fill out an Equipment in Transit form refer to Appendix B for a copy of the form 38 MRX Microplate Reader User Manual 4 3 Repacking the Reader 4 3 Repacking the Reader This procedure assumes that the original Reader packaging materials are being used Figure 13 summarizes the repacking instructions 1 Remove the optics door and take out the lamp and filters When you replace the optics door insert the optics door packing materials Pack the lamp and each of the filters separately Make that there is no microplate in the plate carrier and no disk in the floppy drive Place the Reader in the original anti static plastic bag Place the bottom end caps into the carton and lower the Reader into the carton Fit the top end caps to the Reader Place the filters and lamp in the accessories box and fit this between the end caps Seal the box carefully and securely Make sure that the box is not dropped or handled in a rough manner Figure 13 Repacking the Reader MRX Microplate Reader User Manual 39 4 4 Replacing the Lamp 4 4 Replacing the Lamp Warning If the Reader has been used recently the optics door and the lamp in the optics assembly may still be hot Allow time for the Reader to cool before touching it Take care when removing the optics door as there may be broken glass in the optics assembly Figure 14
40. ter to it 33 electrical specifications 51 features of 7 8 front panel 15 hardware specifications 49 interface 51 keypad and display 15 performance specifications 49 50 physical environmental conditions 52 repacking the 39 resetting its memory 29 self tests 19 software specifications 49 switching it on 18 unpacking the 11 Repacking the Reader 39 Repeatability Mode 31 Resetting the Reader memory 29 S Safety Information 3 4 Self tests 19 27 43 sample printout 44 Setup menu 21 22 Specifications electrical 51 hardware 49 interface 51 performance 49 50 physical environmental 52 software 49 switching between DOS applications 20 System Menu 25 26 V V Voltage Selector 14 AN Warning Symbol 5 Windows QWERTY Keyboard Option 16 MRX Microplate Reader User Manual Microplate Reader User Manual 105202A MRX Microplate Reader User Manual 65
41. tives available To change the display language from English to Spanish Select SYSTEM from the Setup menu to access the System menu Select LANGUAGE from the System menu to access the Language menu The default or previously defined menu language is displayed LANGUAGE ENGLISH 4 45P 30 06 94 ENTER CE To access the alternative languages select CE LANGUAGE 4 45P 30 06 94 ENGLISH FRANCAIS DEUTSCH ITALIANO OTHER Select one of the languages displayed by pressing the function key directly below it or select OTHER to access the Spanish option LANGUAGE 4 45P 30 06 94 ESPANOL CE To select Spanish as the display language press the function key below ESPANOL The menus will then be displayed in Spanish MRX Microplate Reader User Manual 2 2 Setup Procedures Filters Menu The Filters menu allows you to install new filters and enter filter wavelengths Select FILTERS from the Setup menu to display the number of filters installed NO OF FILTERS 2 4 45P 30 06 94 ENTER CE ENTER Confirm the number of filters shown CE Clear the number of filters and enter a new number Minimum 1 filter Maximum 6 filters Once the number of filters has been entered the Reader prompts you to enter the wavelength of each filter in turn Note When installing new filters remember that the filter with the lowest wavelength should be in position 1 on the filter wheel and so on in ascending order of wavelength The Reader will
42. tric important for all users to be aware of the potential hazard of using liquids ae in close proximity to an electrical supply If any liquids are spilled azar disconnect the instrument from the mains electrical supply immediately and clean the liquid up DO NOT reconnect the electrical supply until the instrument has been fully inspected by an approved service engineer Take care when using non rigid non standard microplates as spillages are more likely to occur To switch this instrument off press the power switch on the front panel Remove the mains power cable from the socket on the rear panel Warning Operating this equipment in ways other than detailed in this manual may Incorrect impair the protection provided by the instrument Operation e DO NOT operate this equipment with the covers removed as potentially lethal voltages are contained within e DO NOT operate this equipment with the safety earth ground disconnected e DO NOT install unauthorized cards spare components or accessories as this may impair the safety of the Reader and will invalidate its watranty e DO NOT place any objects or stand within 150mm of the front of the Reader as this will obstruct the plate carrier e DO NOT overfill the microplate wells as this may lead to liquid contamination of the wells and impair the safety of the Reader e BE PREPARED for unexpected plate carrier movement when the Reader is controlled by an external computer
43. ts These cover A D Status e Begins conversion and checks that the status line goes high and low e Verifies that hardware can be detected and chosen Plate Motor e Moves the plate to Out position and then to the In position e Verifies that the plate carrier position sensors are operating correctly e Checks motor and sensors Background e Checks for light leakage into the reading Light compartment Moves the filter motor to the first position e Verifies that current is flowing through the bulb when it is switched on e Checks that the bulb has not blown KH e Checks the filter motor and optical alignment Transmission le Verifies that all fibres are capable of Levels transmitting at least 33 of the maximum transmission level e Checks for blocked channels or broken diodes e Checks that gains can be selected for all filters e Checks for damaged or missing filters MRX Microplate Reader User Manual 43 Appendix A Self Test Diagnostics Self Test Sample Printouts SELF DIAGNOSTIC SEQUENCE Reader Serial 2CXC2549 Technologist Date 3 17 98 Time 11 15 02 AM Test 1 A D Pass Test 2 Plate motor Pass Test 3 Background light levels Pass Test 4 Bulb Pass Test 5 Filter motor Pass Test 6 Transmission levels Pass Test 7 Filters Pass Self Diagnostic Summary Reader Status Ready 44 MRX Microplate Reader User Manual Appendix B Access
44. y 8 columns format and the other is the 10 rows by 4 columns format or the Chinese plate format The Reader will display the current plate type setting MRX Microplate Reader User Manual 2 2 Setup Procedures Select ENTER to confirm the current plate type setting or CE to access the options PLATE TYPE 4 45P 30 06 94 12x8 10x4 CE Select 12x8 or 10x4 to the specify the plate type Repeatability Mode Using the repeatability mode you can specify that each reading the Reader takes is repeated a number of times and averaged into a single result The Reader will display the current repeatability setting Select ENTER to accept the current setting and return to the Setup menu or select CE to enter a different number REPEATABILITY MODE 4 45P 30 06 94 ENTER CE Minimum 1 no reading repeats Maximum 4 Le Note Repeated readings will require a longer reading time and this option should be used with caution for time critical plate readings MRX Microplate Reader User Manual 31 2 2 Setup Procedures This page is intentionally left blank 32 MRX Microplate Reader User Manual Chapter 3 Printer Interface Chapter 3 Printer Interface If the Reader has been supplied with a printer this printer will be completely compatible with it The following information is for users whose printer was not supplied with the Reader Any printer which communicates through a Centronics parallel printer interface will op
45. y a maintenance reminder HAVE YOU PERFORMED YOUR MAINTENANCE CHECKS To clear the message press any key The reminder is intended for use by laboratories which routinely verify the operation of their instruments The self test printouts and the frequency of the maintenance reminders may be altered from the SETUP menu Main Menu If no faults are detected the instrument displays the Main menu which shows all the available software programs and SETUP If there are more MRX Microplate Reader User Manual 19 2 1 Installation than four software programs installed use the lt and gt cursor keys to view them While the Main menu is displayed the plate carrier can be moved in using the A cursor key and out using the v cursor key The plate carrier should not be left out of the Reader for more than a few minutes at a time as it will gather dust and may become damaged If it is left for five minutes without using the software the Reader will move the plate carrier in automatically Switching between DOS Applications On a MR Su 1 Press the Esc key to return the Reader to the Main menu 1 Switch the Reader off 1 Change the software disk 1 Switch the Reader on again Le Note If you have changed any settings in the Setup menu you will have to re enter them for the new program On a MRXp pius 1 Press the Esc key to return the Reader to the Main menu 1 Select the required program using one of the function keys 2
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