Finnpipette F1 ClipTip User Manual
Contents
1. Picture 16 Make sure that the tip fittings are aligned in same level adjust if necessary 8 Sterilization Before autoclaving disassemble the tip fitting from the tip cone and autoclave them separately After autoclaving the tip cone and tip fitting must be cooled to room temperature for at least two hours When reassembling make sure that the tip cone and tip fitting are dry We recommend checking the calibration after every sterilization cycle The effectiveness of the autoclaving must be verified by the user 8 1 Autoclavable parts Single and multichannel pipettes Tip fitting 5 Tip fitting O ring 5 1 single channel pipettes ip cone assembly 42 Parts can be repeatedly autoclaved at 121 C 252 F 2 ata for 20 minutes 9 Troubleshooting The table below lists possible problems and their solutions Defect Possible reason Possible action Leakage e ip fitting incorrectly attached or gt Re attach tighten the tip fitting loose or replace with new tip fitting s using the tool e ClipTip clips are bent Discard the tip e ip fitting o ring damaged Change the O ring e ip incorrectly attached Attach firmly e Foreign particles between the tip Clean the tip cones and attach and the tip cone new tips e Foreign particles between the Clean and grease the O ring and piston the O ring and the cylinder cylinder e nsufficient amount of grease on the Grease accordingly cylinder and2. jenuew 1esf DW Thermo Scientific F1 ClipTip Single Channel Multichannel Instructions for Use This product complies with the European Union Directive 98 79 EC and it is marked with a CE marking When the product is used in applications related to the Directive 98 79 EC read the additional information enclosed in the product package or contact the manufacturer at info pipettesOthermofisher com to ensure correct and safe use The CE mark covers the system containing the CE marked F1 ClipTip pipette and the CE marked ClipTip pipette tips Product specifications are subject to change without prior notice ClipTip is a registered trademark of Thermo Fisher Scientific Inc and its subsidiaries 1 Introduction Congratulations on your purchase of a Thermo Scientific F1 ClipTip pipette The F1 ClipTip is a general purpose air displacement micropipette Its intended use is for aspirating and dispensing liquids The F1 ClipTip features an innovative interlocking tip interface technology that locks the tip in place ensuring secure tip attachment and preventing tips from being displaced in the middle of pipetting F1 ClipTip operates only with ClipTip tips For improved ergonomics tips are attached and ejected using minimal application pressure The F1 ClipTip product offering covers a volume range from 1 ul to 1000 ul and includes variable and fixed volume single channel pipettes as well as multichannel pipettes The convenient col
3. 1 0028 1 0029 1 0030 1 0031 1 0032 1 0033 1 0034 1 0036 1 0037 1 0038 1 0039 1 0040 1 0042 1 0043 1 0044 1 0046 1 0047 1 0048 1 0050 1 0051 1 0053 1 0054 Appendix 2 Manufacturer specification limits Thermo Scientific F1 ClipTip Variable Volume Single Channel Compatible Order No Range Volume Inaccuracy Imprecision ClipTip ul ul SDyul 4641170 1 10 ul 10 E10 0 EE 00 0 050 0 50 20 9 SEU aU 0040 0 80 1 SUZ EA yt 0 020 2 00 4641180 2 20pl 20 020 1 00 0 08 0 40 20 10 190 0 06 0 60 2 20 00 00 DONS 4641190 5 50 ul 50 030 060 DST CT 50 25 M 7 0 13 0 50 5 40 15 3 00 QUIS ELS 4641200 10 100 ul 100 080 080 0 20 0 20 200 50 0 60 1 20 0 20 0 40 10 EEUU SU 0 10 1 00 4641210 20 200 ul 200 E 0 60 0 4 0 20 200 100 L00 0 4 0 40 20 20 30 ol 0 14 0 70 4641220 30 300 ul 300 ho 0 60 0 6 0 20 300 150 0 6 0 40 30 1045 11 50 0 18 0 60 4641230 100 1000 ul 1000 6 0 0 60 20 0 20 1000 500 4 0 0 00 0 30 100 D 1 00 0 6 0 60 Thermo Scientific F1 ClipTip Fixed Volume Single Channel Compatible Order No Volume Inaccuracy Imprecision ClipTip ul ul SD ul CV 4651170 0 040 400 0 040 4 00 20 4651180 5 0 070 1 40 0 070 1 40 20 4651190 10 68 0 080 0 80 20 4651200 20 0 14 0 70 0 100 0 50 20 4651210 25 015 0 60 175 0 50 50 4651220 50 0 30 0 60 0 20 0 40 50 4651230 100 0 40 0 40 0 30 0 30 200 4651240 200 0 80 0 40 0 600 0 30 200 4651250 250 210 0 40 075 0
4. polyethylene 4 Safety Cautions are marked with this symbol 4 1 Intended use The intended use of the device is to transfer liquids in the volume range of 1 ul to 1000 yl The F1 ClipTip pipette and ClipTip tip are designed as a component of an analyzing system for an end user who is responsible for validating the system to ensure reliable and safe results Damage to health Follow general procedures for hazard prevention and safety instructions e g wear protective clothing eye protection and gloves For use and waste disposal of hazardous e g radioactive and potentially infectious material follow the safety instructions and general laboratory practice p The pipette and tips are not intended for in vivo use Do not use the pipette for pipetting any liquid to be injected into a human body gt Do not eject the tip towards anybody The F1 ClipTip is to be used by trained personnel with required laboratory skills The instructions for use must be read prior to and during the use of the device pipette and tip The F1 ClipTip pipette can be used between 4 C and 40 C Incorrect dispensing results Performance may vary due to a pipetting method forward pipetting technique recommended b temperature air liquid vessel pipette and tip C pressure d humidity e operator e g thumb movement pipetting angle f liquid density viscosity and vapor pressure g type of tip gt f the pipetting perfor
5. 0 ES 15 5 100 1000 ul 1000 80 0 8 30 0 3 1000 blue 100 8 0 8 0 30 Eu Thermo Scientific F1 ClipTip Fixed Volume Single Channel Compatible Fixed Volume Inaccuracy Imprecision ClipTip ul ul SD ul CV 1 0 050 5 00 0 050 5 00 20 5 E4078 12 50 0 075 1 50 20 10 0 120 1 20 0 080 0 80 20 20 0 20 1 00 0 10 0 50 20 25 0 50 2 00 0 20 0 80 50 50 0 50 1 00 0 20 0 40 50 100 0 80 0 80 0 30 0 30 200 200 1 60 0 80 0 60 0 30 200 250 4 00 1 60 Non 0 60 300 500 4 00 0 80 1 50 0 30 1000 1000 8 00 0 80 3 00 0 30 1000 Thermo Scientific F1 ClipTip Variable Volume Multichannel Compatible Range Channel Volume Inaccuracy Imprecision ClipTip Color code ul ul SDul CV 1 10u 812 10 7 016 16 20 pink 1 024 24 0 16 16 5 50 8 12 50 25240 04 0 8 50 violet 5 1 0 20 0 4 8 0 10 100 ul 8 12 ES 200 yellow 10 ST 30 300 ul 6 12 300 8 0 E 3 0 1 0 300 orange 30 6 0 M 10 10 0 20 Appendix 4 Assembly and spare parts Available as a spare part See model specific order numbers on page 26 1062800 1062930 Service and Multichannel service tool adjustment tool 4 Handle 5 Tip fitting 42 Tip cone assembly 50 Module 21 1 10yl 2 20pl 1ul 5ul 10ul Fixed 20 ul Fixed lt 14 lt a 14 ES 15 Ss 16 17 17 _ 18 19 18 20 19 21 E 20 22 23 B 2 5 50yl 25 50 ul Fixed 14 T 15
6. 1000 ul 1000 ul Thermo Scientific ClipTip filter tips sterile ClipTip Volume 20 ul 50 ul 200 yl 300 yl 1000 ul Thermo Scientific ClipTip racked and sterile tips Oty 10x96 rack 10x96 rack 10x96 rack 10x96 rack 10x96 rack 10x96 rack 10x96 rack 10x96 rack 8x96 rack 8x96 rack Oty 10x96 insert 10x96 insert 10x96 insert 10x96 insert 10x96 insert 10x96 insert 10x96 insert 10x96 insert 8x96 insert 8x96 insert Oty 10x96 rack 10x96 rack 10x96 rack 10x96 rack 8x96 rack All ClipTip tips are certified to be free from RNase DNase DNA ATP and pyrogens 27 Appendix 6 F1 ClipTip and ClipTip compatibility table Non filter tips Filters F1 ClipTip 1 10 F1 SIUE 2 20ul ClipTip Filter 20 ClipTip Filter 50 ClipTip Filter 200 ClipTip Filter 300 ClipTip Filter 1000 ClipTip 200 ClipTip 300 ClipTip 1000 ClipTip 20 ClipTip 50 F1 ClipTip ipl fixed volume F1 ClipTip Sul fixed volume an fixed volume 28 Appendix 7 Pipette accessories Multichannel stand 9420390 F stand 9420400 Reagent reservoir 100 ml 95128085 Y Reagent reservoir 25 ml 95128093 M j I Reagent reservoir 25 ml divided 77951228095 29 info pipettes thermofisher com www thermoscientific com GLP www thermoscientific com thermoscientific com 2015 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific Inc
7. 28 0 1 0046 DU 1 0047 230 1 0049 29 5 1 0050 30 0 1 0052 85 1 0018 1 0019 1 0020 1 0020 1 0021 1 0022 1 0023 1 0024 1 0025 1 0026 1 0027 1 0028 1 0029 1 0030 1 0031 1 0032 1 0033 1 0035 1 0036 1 0037 1 0038 1 0040 1 0041 1 0042 1 0044 1 0045 1 0046 1 0048 1 0049 1 0051 1 0052 10 Appendices Appendix 1 Conversion table Air pressure kPa 90 95 TOOTO OS 1 0019 1 0020 1 0020 1 0021 1 0021 1 0021 1 0022 1 0022 1 0023 1 0023 1 0023 1 0024 1 0024 1 0025 1 0025 1 0026 1 0026 1 0027 1 0027 1 0028 1 0028 1 0029 1 0029 1 0030 1 0031 1 0031 1 0032 1 0032 1 0033 1 0033 1 0034 1 0034 1 0035 1 0036 1 0036 1 0037 1 0038 1 0038 1 0039 1 0039 1 0040 1 0041 1 0041 1 0042 1 0043 1 0043 1 0044 1 0045 1 0046 1 0046 1 0047 1 0047 1 0048 1 0049 1 0050 1 0050 1 0051 1 0052 1 0053 1 0053 100 1 0020 1 0020 1 0021 1 0022 1 0023 1 0024 1 0025 1 0025 1 0026 1 0027 1 0028 1 0029 1 0031 1 0032 1 0033 1 0034 1 0035 1 0036 1 0037 1 0039 1 0040 1 0041 1 0042 1 0044 1 0045 1 0047 1 0048 1 0049 1 0051 1 0052 1 0054 101 3 1 0020 1 0020 1 0021 1 0022 1 0023 1 0024 1 0025 1 0026 1 0027 1 0028 1 0029 1 0030 1 0031 1 0032 1 0033 1 0034 1 0035 1 0036 1 0038 1 0039 1 0040 1 0041 1 0043 1 0044 1 0045 1 0047 1 0048 1 0050 1 0051 1 0052 1 0054 for distilled 105 1 0020 1 0021 1 0022 1 0022 1 0023 1 0024 1 0025 1 0026 1 0027
8. 30 300 4651260 500 215 0 30 9 0 30 1000 4651270 1000 230 0 30 SCH 0 30 1000 Thermo Scientific F1 ClipTip Variable Volume Multichannel Compatible Order No Range Channel Volume Inaccuracy Imprecision ClipTip ul ul SD ul CV 4661110 1 10 yl 8 10 0 240 240 0160 1 60 20 J 20 200 400 0 150 3 00 0 120 1200 0 080 8 00 4661120 5 50pl 8 50 O 50 29 oe A J LE O O 100 4661130 10 10011 8 100 130 E 50 SEE OU TU 10 UE ONU 00 4661140 30 30011 8 300 el IS DO 150 oss 1 30 SECH 00 4661150 1 10 yl 12 10 40 240 240 0160 160 20 J 0 200 400 0 150 3 00 1 0 120 1200 0 080 8 00 4661160 5 50 ul 12 50 A 50 25 0O20 O 70 5 A EESTI 4561170 10 10011 12 100 0 ee 50 zs xL DEE 10 II e 4661180 30 500 ul 12 300 430 1 00 09 030 300 150 E Op 0 30 SETA i500 06 200 Appendix 3 1508655 calibration specifications Thermo Scientific F1 ClipTip specifications according to 1508655 standard Thermo Scientific F1 ClipTip Variable Volume Single Channel Compatible Range Volume Inaccuracy Imprecision ClipTip Color code ul ul SD ul CV 1 10 ul 10 EG 0 080 0 8 20 pink 1 EE 0 080 8 0 2 20 pl 20 0 20 EU 0 10 0 5 20 pink 2 0 20 10 0 0 10 g0 EDU 50 0 50 1 0 0 20 0 4 50 violet 9 30 50 10 0 20 4 0 10 100 pl 100 0 80 0 8 0 30 200 yellow 10 0 80 8 0 0 30 20 200 ul 200 1 60 0 8 0 60 200 yellow 20 1 60 8 0 0 60 3 0 30 300 ul 300 4 0 t3 0 5 300 orange 30 4
9. and its subsidiaries Specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Thermo Fisher Scientific Ratastie 2 P O Box 100 FI 01621 Vantaa Finland Thermo SCIENTIFIC 1508810 05 Part of Thermo Fisher Scientific
10. components which contain the antimicrobial additive silver sodium hydrogen zirconium phosphate Silver is known to inhibit the growth of a broad spectrum of micro organisms For waste disposal instructions contact your local environmental agency For more information contact us at info pipettesOthermofisher com Common laboratory disinfectants such as 70 96 ethanol can be used without effect on antimicrobial treatment IN Aggressive substances can damage the pipette or pipette parts Check for material compatibility before using organic solvents and aggressive chemicals ClipTip pipette tips are made of natural color virgin polypropolyene The tips feature a hydrophobic surface which effectively prevents liquid retention ClipTips pipette tips come in multiple packaging options including racks and reload packs in sterile and non sterile versions ClipTip tips are also available with a filter and are certified free of human DNA RNase DNase ATP and endotoxin contamination The material used in F1 ClipTip pipettes and ClipTip tips are as follows F1 CLIPTIP PIPETTES Material Component External surfaces of the upper parts ABS PC acrylonitrilebutadienstyrene polycarbonate PA polyamide PEI polyetherimide Foil Exterior and interior of lower parts PEI polyetherimide PA polyamide PVDF polyvinyldenfluoride EPDM ethylene propylene diene rubber FVM fluorosilicone rubber oteel stainless steel CLIPTIP TIPS Tip ES polypropylene Filters
11. volume and the selected volume of a pipette A V W A inaccuracy V mean volume Vo nominal volume Inaccuracy can be expressed as a relative value A 100 x A Vo Imprecision random error Imprecision refers to the repeatability of the pipettings It is expressed as standard deviation S or coefficient of variation CV 5 standards deviation V mean volume n number of measurements Standard deviation can be expressed as a relative value CV 100 x S IN 7 Maintenance Cautions for incorrect maintenance The pipette should be regularly serviced according to the instructions for use The pipette performance and functioning must be checked after maintenance Aggressive substances can damage the pipette or pipette parts Check for material compatibility before using organic solvents and aggressive chemicals Use only original Thermo Scientific spare parts and ClipTip tips Avoid excess grease Use only the grease provided with the pipette If the pipette is used daily it is recommended to service the pipette every three months When the F1 ClipTip is not in use make sure it is stored in an upright position We recommend a pipette stand for this purpose When shipping the device for service or inspection ensure that it is completely free of chemical biological or radioactive contamination 1 1 Daily maintenance The pipette should be checked at the beginning of each day for dust and dirt on its outside surf
12. 1 300 _ Loop _ _ 9 22 em 5 2 s 5 1 10 100 20 2001 30 3001 100 1000 100 200 ul Fixed 250ul Fixed 500 1000 ul Fixed 12 19 13 13 a 14 15 6 17 11 11 23 24 F1 ClipTip single channel and multichannel spare part order numbers Single Channel 1 10ul 1ul 5ul 10ul Fixed 4 2214000 1 10ul 4 2214070 Fix 11 4 2214080 Fix 5 4 2214090 Fix 10 5 11072080 5 1 2214930 3 pcs 5 2 1030060 18 1030380 19 1030060 23 1030170 42 2214180 2 20ul 20pl Fixed 4 2214010 2 20yI 4 2214100 Fix 20 5 11072080 5 1 2214930 3 pcs 5 2 1030060 18 1030380 19 1033110 42 2214190 5 50ul 25yl 501 Fixed 4 2214020 5 50ul 4 2214110 Fix 2511 4 2214120 Fix 50 5 11072080 5 1 2214930 3 pcs 5 2 1030060 18 1030500 19 1039060 42 2214200 10 100 4 2214030 10 1001 5 11072080 5 1 22149303 pcs 5 2 1030060 17 1030510 42 2214210 20 200 100ul 2001 Fixed 4 2214040 20 200yl 4 2214130 Fix 100 4 2214140 Fix 200 5 11072080 5 1 2214930 3 pcs 5 2 1030060 17 1030160 42 2214220 26 30 300ul 2501 Fixed 4 2214050 30 3001 4 2214150 Fix 250 5 11072080 5 1 2214930 3 pcs 5 2 1030060 17 1033330 42 2214230 100 1000ul 5001 10001 Fixed 4 2214060 100 100011 4 2214160 Fix 500 4 2214170 Fix 1000ul 5 11072200 5 1 2215540 3 pcs 5 2 1033410
13. 17 1030020 42 2214240 Multichannel 8 ch 12 ch 4 2214000 1 10yl 4 2214020 5 50ul 4 2214030 10 100ul 4 2214050 30 3001 5 11072080 5 1 2214920 12 pcs 5 2 1030060 42 2214390 1 10ul 12 pcs 42 2214400 5 50ul 12 pcs 42 2214410 10 1001 12 pcs 42 2214420 30 3001 12 pcs 8 ch 50 2213620 1 10 50 2213630 5 50yl 50 2213640 10 100ul 50 2213650 30 3001 12 ch 50 2213660 1 10yl 50 2213670 5 50yl 50 2213680 10 100 50 2213690 30 3001 Appendix 5 ClipTip ordering information Code 94410210 94410213 94410250 94410253 94410310 94410313 94410510 94410513 94410710 94410713 Code 94410217 94410218 94410257 94410258 94410317 94410318 94410517 94410518 94410717 94410718 Code 94420213 94420253 94420313 94420513 94420713 Clip ClipTip 20 ClipTip 20 sterile ClipTip 50 ClipTip 50 sterile ClipTip 200 ClipTip 200 sterile ClipTip 300 ClipTip 300 sterile ClipTip 1000 ClipTip 1000 sterile Clip Tip ClipTip 20 ClipTip 20 sterile ClipTip 50 ClipTip 50 sterile ClipTip 200 ClipTip 200 sterile ClipTip 300 ClipTip 300 sterile ClipTip 1000 ClipTip 1000 sterile ClipTip Filter 20 ClipTip Filter 50 ClipTip Filter 200 ClipTip Filter 300 ClipTip Filter 1000 Volume 20 ul 20 ul 50 ul 50 ul 200 ul 200 ul 300 ul 300 ul 1000 ul 1000 ul Thermo Scientific ClipTip reload inserts Volume 20 ul 20 ul 50 ul 50 ul 200 ul 200 ul 300 ul 300 ul
14. 17 the bigger O ring 18 and the smaller O ring 19 back onto the piston 12 Compress the spring 13 with your fingers by pressing the piston and the spring support 16 against each other and then slide the tube 20 with the remaining parts onto the piston Hold the spring 13 compressed and carefully slide the entire assembly into the tip cone 11 and then release the spring Put the spring 15 and support 14 on top of the tip cone and carefully insert the tip cone assembly 42 onto the handle 4 and tighten it by hand Reassemble the tip ejector 10 Put the tip fitting 5 onto the end of the tip cone assembly and tighten it with the tool We recommend using a new O ring during each maintenance 12 2 20yl 2011 Fixed 5 50yl 25 50 Fixed Slide the spring 13 spring support 16 and tube 17 back onto the piston 12 Compress the spring 13 with your fingers by pressing the piston 12 and the spring support 16 against each other and then slide the bigger O ring 18 the smaller O ring 19 the spring support 20 and spring 21 smaller diameter against spring support 20 onto the piston Hold the spring 13 compressed and carefully slide the entire assembly into the tip cone 11 and then release the spring Put the spring 15 and support 14 on top of the tip cone 11 and carefully insert the tip cone assembly 42 onto the handle 4 and tighten it by hand Reassemble the tip ejector 10 Put the tip fitting 5 onto the end of the tip cone assembly and tighten i
15. aces Particular attention should be paid to the tip cone To clean the pipette we recommend wiping it with a napless cloth wetted with 70 ethanol Leakage test www www A leakage test can be performed at any time It is recommended to be carried out after maintenance or autoclaving Pre wet the tip three to five times with water Aspirate a nominal volume of water into the tip Place the pipette on a stand or keep it firmly in your hand Wait for 20 seconds If a drop falls within 20 s the pipette is leaking If the pipette leaks see Chapter 9 Troubleshooting for possible reasons and solutions 7 2 Periodic maintenance The maintenance procedure starts with the disassembly of the pipette www F1 ClipTip O rings 5 1 are wear parts It is recommended to replace O rings regularly to ensure proper sealing Always replace the O rings if they are worn or damaged 1 2 1 Disassembly of single channel pipettes 1 Remove the tip fitting 5 Picture 8 To remove the tip fitting place the end of tool 3 Picture 9 without a metal rod on the tip fitting tool against the tip fitting The tip fitting will guide the tool properly into place Turn the tool counterclockwise Y turn to untighten the tip fitting Rotate the tip fitting tool and press the end with a metal rod through the tip fitting Be careful not to damage the O ring inside the system with excess force Loosen the tip fitting with a tool and pull it out Make sure that the O
16. atible range ClipTip 4661110 FI ClipTip 8 ch1 10 8 1 10 ul 0 02 ul pink ClipTip 20 4661120 F1 ClipTip 8 ch 5 50 ul 8 5 50 ul 0 1 yl violet ClipTip 50 4661130 F1 ClipTip 8 ch 10 100 ul 8 10 100 ul 0 2 yl yellow ClipTip 200 4661140 F1 ClipTip 8 ch 30 300 ul 8 30 300 yl 1 ul orange ClipTip 300 4661150 F1 Cliplip 12 ch 1 10 ul 12 1 10 ul 0 02 ul pink ClipTip 20 4661160 F1 Cliplip 12 ch 5 50 ul 12 5 50 ul 0 1 ul violet ClipTip 50 4661170 F1 Cliplip 12 ch 10 100 ul 12 70 100 ul 0 2 ul yellow ClipTip 200 4661180 F1 ClipTip 12 ch 30 300 ul 12 30 300 ul 1 ul orange ClipTip 300 2 Package The F1 ClipTip package contains the following items 1 F1 Cliplip pipette oervice tool Multichannel service tool Tip fitting tool ClipTip tip sample Tube of grease Order No 2203130 Instructions for Use Calibration certificate Warranty Certificate 9 Shelf hanger Order No 2206040 10 Warning labels 11 O ring Order No 2214930 Single Channel Pipette Scp 300 3 pcs Order No 2215540 Scp 1000 3 pcs Order No 2214920 Multichannel Pipette Mcp 12 pcs 3 Main Illustration 100 02 Fi hp Pipetting and volume adjustment button Adjustable finger rest Tip ejector Volume display Space for personal ID tag Tip fitting Tip sealing O ring Color coding NOS RHO 3 1 Materials The F1 ClipTip is made of mechanically durable and chemically resistant materials The F1 ClipTip pipette includes
17. djustment of the pipette is correct Pipette adjustment Adjustment is done only for one volume The recommended adjustment volume is the minimum volume or 10 of the maximum volume 1 Fit the service tool Picture 7 into the openings of the calibration nut at the top of the handle 2 Turn the service tool clockwise to increase or counterclockwise to decrease the volume 3 After adjustment check the calibration according to the instructions above Formulas for calculating results Conversion of mass to volume Picture 7 V w e xZ V volume ul W weight mg e evaporation loss mg Z conversion factor for ul mg conversion Evaporation loss can be significant at low volumes To determine mass loss dispense water into the weighing vessel note the reading and start a stopwatch Check and note down how much the reading decreases during 30 seconds e g 6 mg 0 2 mg s Compare this to the pipetting time from taring to reading Typically the pipetting time might be 10 seconds and the mass loss is then 2 mg 10 s x 0 2 mg s in this example If an evaporation trap or lid on the vessel is used the correction of evaporation is usually unnecessary The factor Z is for converting the weight of the water to volume at the test temperature and pressure A typical value is 1 0032 ul mg at 22 C and 95 kPa See the conversion table in Appendix 1 Inaccuracy systematic error Inaccuracy is the difference between the dispensed
18. h the tip guide the pipette into a ClipTip in a rack and press with light force until the tip is attached delicate click sound will indicate that the tip is attached The tip is attached when the clips Picture 4 are locked to the tip fitting Do not use excessive force when attaching the tips as the device requires minimum attachment force Lift the pipette If the tip is not attached repeat Steps 1 and 2 Use only ClipTip tips with an F1 ClipTip pipette Check that all clips are locked to ensure tip sealing Picture 4 ClipTip tips are designed for single use only To ensure sterility and purity of tips check that the package is unbroken For sterile tips also ensure that the indicator dot is red Check that the tip is complete and unbroken Check for foreign particles in the tip Check that the filter tips have filters in place and that each filter is unbroken and not tilted 5 5 Aspirating and dispensing liquid co 5 Fill a suitable vessel with the liquid to be dispensed Depress the pipetting button to the first stop Picture 5 Dip the tip under the surface of the liquid 1234 in the reservoir to a depth of about 1 cm and slowly release the button Withdraw the tip from the liquid by moving it against the edge of the reservoir to remove excess liquid Deliver the liquid by gently depressing the button to the first stop After a delay of about one second continue to depress the button all the way to t
19. he pipette Follow the ergonomic guidelines for laboratory work to minimize the risk of repetitive strain injury RSI 5 1 ID tag You can mark the pipette ID tag with your initials pipetting application or calibration date Remove the pipettes module to replace the tag Mark the label and slide the tag together with the holder back to its slot Picture 1 5 2 Adjusting the finger rest The finger rest supports the position of the pipette on the index finger enhancing ergonomics The finger rest can be adjusted by rotating it 60 degrees in both directions from the center position Picture 2 Usually right handed operators turn it left counter clockwise to achieve the best possible position for the thumb to eject the tip vv w w 5 3 Setting the delivery volume 1 3 4 NOTE Do not set volumes outside the pipette s specified volume range Using excessive force to turn the volume adjustment button outside the range may jam the oet the delivery volume using the volume adjustment button on the top of the pipette Picture 3 Pull the button to activate the volume setting To increase the delivery volume turn the button counterclockwise To decrease the delivery volume turn it clockwise Lock the volume by pushing the button down Check the volume on the display Picture 3 mechanism and eventually damage the pipette 5 4 Tip attachment 1 2 3 Incorrect use of tips www attac
20. he second stop This action will empty the tip Withdraw the tip by moving It against the edge of the target container Picture 5 Ensure proper functioning of F1 ClipTip 1 10ul multichannel and 1 50 pul single channel pipettes The F1 ClipTip 1 10ul multichannel and 1 50 ul single channel pipettes utilize an innovative super blow out piston technology to ensure efficient liquid delivery with small volumes In pipettes with the super blow out piston three separate movements are observed Picture 6 when the plunger is pressed down to dispense liquid 2 4 Primary plunger movement delivers the set volume amount Blow out additional phase to dispense residual liquid super blow out enhances the effectiveness of blow out 8 Picture 6 Plunger in up position Plunger in down position When the pipette is not used for a period of time the plunger movement may become stiff due to hardening of grease In rare cases this can affect the functionality of the super blow out piston which can become temporarily jammed To ensure proper functionality it is recommended to press the plunger several times to the final stop step 3 in picture before dosing the sample liquid This releases the piston and re distributes the lubricant NOTE If the super blow out piston is jammed increased force might be required to release it Ensure that plunger moves to the down position Picture 6 For more information about different pipetting
21. mance is critical to the outcome of a specific application the result has to be assured with an alternative test and if this is not an option by duplicate testing The possibility of an incorrect volume delivery during pipetting cannot be entirely mitigated To avoid inaccurate dispensing and or leakage check that the tip is properly attached to the pipette When rotating the volume adjustment button do not exceed the volume range of the pipette Otherwise the pipette may be damaged or affect pipetting performance Pipetting performance cannot be guaranteed if the tip is reused Choose only a tip and pipette with matching color coding 5 Operation Cautions for incorrect operation gt For optimum performance make sure that the tips pipette and solution are at the same temperature Before you begin your actual pipetting fill and empty the tip three to five times with the solution that you will be pipetting p Push and release the pipetting button slowly at all times particularly when working with high viscosity liquids Never allow the pipetting button to snap back gt Do not hold the pipette horizontally or upside down when there is liquid inside the tip Sample liquid may enter the pipette which will affect the pipetting performance and may damage the pipette gt Operate the pipetting button gently If it is quickly released sample liquid may enter the pipette which will affect the pipetting performance and may damage t
22. ol clockwise stop tightening when you feel resistance Rotate the tip fitting tool and tighten the tip fitting by using the end without a metal rod Check that there is no gap between tip fitting and cylinder Picture 12 Fit in a new O ring 5 1 No gap Be careful not to overtighten the tip fitting This may damage the thread in the pipette NOTE If the tip fitting is tightened using the end with the metal rod of tip fitting tool the tool will start to slip This may disform the tool and make it more difficult to use 7 2 3 Disassembly of multichannel pipettes 1 Remove and discard the O ring and remove the tip fittings Picture 8 with the Tip fitting tool Picture 9 We recommend replacing the O ring during each maintenance 2 Press the tip ejector 4 1 down whereupon it presses the tip ejector 23 down at the same time Keep the ejector cover manually in the down position and let the tip ejector rise to the normal position whereupon the parts snap apart revealing a slot 7 If needed facilitate lightly by hand 3 Keep the handle 4 still and insert the second end of the service tool 2 into the slot and turn clockwise to separate the handle and the module 6 from each other Loosen until you can pull the parts apart 4 Pullthe holder spring 13 downwards and remove the two locking pieces 12 Finally remove the holder spring carefully ensuring that the spring does not fly off 5 Remove the two MCP adap
23. or coding system facilitates to identify the correct by volume for use F1 ClipTip Variable Volume Single Channel Cat no Description Volume range Increment Color code Compatible ClipTip 4541170 F1 Cliplip 1 10 ul 1 10 ul 0 02 ul pink ClipTip 20 4641180 F1 ClipTip 2 20 pl 2 20 ul 0 02 ul pink ClipTip 20 4641190 F1 ClipTip 5 50 ul 5 50 ul 0 1 ul violet ClipTip 50 4641200 F1 Cliplip 10 100 ul 10 100 ul 0 2 ul yellow ClipTip 200 4641210 F1 ClipTip 20 200 ul 20 200 ul 0 2 ul yellow ClipTip 200 4641220 F1 Cliplip 30 300 yl 30 300 pl 1 ul orange ClipTip 300 4641230 F1 ClipTip 100 1000 ul 100 1000 ul 1 ul blue ClipTip 1000 F1 ClipTip Fixed Volume Single Channel Cat no Description Volume range Color code Compatible ClipTip 4651170 FI ClipTip 1 ul 1 ul dark blue ClipTip 20 4651180 FI ClipTip 5 ul 5 ul dark blue ClipTip 20 4651190 F1 Cliplip 10 ul 10 ul dark blue ClipTip 20 4651200 FI ClipTip 20 ul 20 ul dark blue ClipTip 50 4651210 FI ClipTip 25 ul 25 ul dark blue ClipTip 50 4651220 FI ClipTip 50 ul 50 ul dark blue ClipTip 50 4651230 FI ClipTip 100 ul 100 ul dark blue ClipTip 200 4651240 F1 Cliplip 200 ul 200 ul dark blue ClipTip 200 4651250 F1 Cliplip 250 pl 250 ul dark blue ClipTip 300 4651260 F1 Cliplip 500 ul 500 ul dark blue ClipTip 1000 4651270 FI ClipTip 1000 ul 1000 ul dark blue ClipTip 1000 color coding does not apply to fixed models F1 ClipTip Multichannel Cat no Description Channels Volume Increment Colorcode Comp
24. pment An analytical balance must be used The scale graduation value of the balance should be chosen according to the selected test volume of the pipette Volume range Readable graduation under 10 ul 0 001 mg 10 100 ul 0 01 mg above 100 ul 0 1 mg The test liquid is deionized distilled grade 3 water in compliance with ISO 3696 Tests are done in a draft free room at a constant 0 5 C temperature between 15 C to 30 C The relative humidity must be above 50 Especially with volumes under 50 ul air humidity should be as high as possible to reduce the effect of evaporation loss Special accessories such as an evaporation trap are recommended 6 3 Procedure to check calibration The pipette is checked at maximum volume nominal volume and minimum volume new tip is first pre wetted three to five times and a series of ten pipettings is done at both volumes A pipette is always adjusted according to the delivery of the selected volume Use of the forward pipetting technique is recommended The maximum permissible errors are valid for the forward pipetting method It is recommended to use the 1508655 calibration limits in Appendix 3 or user defined specifications Procedure 1 Do 10 pipettings at the minimum volume 2 Do 10 pipettings at the maximum volume 3 Calculate the inaccuracy A and imprecision CV of both series 4 Compare the results to the limits If the calculated results are within the selected limits the a
25. ring comes out with the tip fitting Picture 10 52 ec Picture 8 Picture 9 2 Pressthe tip ejector 4 1 Rotate the tip ejector 10 clockwise and pull it out Picture 11 3 Turn the tip cone 11 clockwise with the service tool 1 There are two slots in the service tool the smaller one for pipettes under 300 ul and the bigger one for pipettes over 300 ul 4 Pull out the piston assembly and other parts from the handle with the tip cone 11 Then turn the tip cone upside down and tap all parts from it You can check the parts of each specific pipette from the pictures starting on page 22 Remember to keep all parts in order on the table for reassembly 1 2 2 Cleaning and reassembling single channel pipettes General maintenance instructions can be found on page 10 Clean the piston the piston spring and the O rings with a dry napless cloth Check the tip cone for foreign particles or dirt Immerse it in a suitable cleaning solution Dry before assembly Grease the cleaned parts with the lubricant that comes with the pipette The pipette is assembled in the reverse order to disassembling Check the volume specific reassembling of the piston assemblies starting on page 22 Picture 11 Piston reassembly procedures 1 10pl 1 5 10pl Fixed First slide the spring 21 O ring support 22 and O ring 23 onto the tube 20 Then slide the spring 13 the spring support 16 and tube
26. t with the tool We recommend using a new O ring during each maintenance 10 1001 20 2001 100 20011 Fixed 30 3001 2501 Fixed Slide the spring 13 spring support 16 and O ring 17 back onto the piston 12 Slide the entire assembly into the tip cone 11 Put the spring 15 and support 14 on top of the tip cone 11 and carefully insert the tip cone assembly 42 into the handle 4 and tighten it by hand Reassemble the tip ejector 10 Put the tip fitting 5 onto the end of the tip cone assembly 42 and tighten it with the tool We recommend using a new O ring during each maintenance 100 100011 500 100011 Fixed Put the O ring 17 and support ring 16 onto the tip cone 11 Slide the spring 13 onto the piston 12 and slide the entire assembly into the tip cone 11 Put the spring 15 and support 14 on top of the tip cone 11 and carefully insert the tip cone assembly 42 into the handle 4 and tighten it by hand Reassemble the tip ejector 11 Put the tip fitting 5 onto the end of the tip cone assembly 42 and tighten it with the tool We recommend using a new O ring during each maintenance Reassembly of tip fitting 5 and O rings 5 1 and 5 2 Place the tip fitting onto the tool end with a metal rod so that the screw end is facing upwards Place the O ring into the rod above the tip fitting or make sure that the O ring is at the bottom of the cylinders screw end Align the tool with the cylinder and carefully screw the tip fitting in by turning the to
27. techniques check the Good Laboratory Pipetting Guide available at www thermoscientitific com GLP 5 6 Tip ejection To release the tip point the pipette at a suitable waste receptacle and press the tip ejector with your thumb 6 Calibration and Adjustment 6 1 Factory calibration limits F1 ClipTip pipettes are factory calibrated and adjusted to give the volumes as specified with distilled or deionized water using the forward pipetting technique Manufacturer specifications are shown on the pipette calibration certificate and in Appendix 2 Cautions for calibration and pipette adjustment gt It should be noted that the use of different pipetting techniques may affect the calibration results The pipettes are constructed to permit re adjustment for other pipetting techniques or liquids of different temperature and viscosity The performance of a new and serviced pipette must be checked and documented gt The manufacturer s specifications should be used as guidelines and the user should establish acceptable imprecision and inaccuracy performance limits ISO 8655 gt The user determines the performance and applicability of the pipette and tip combination for a particular application and determine the required interval for performance checking gt Before starting the actual dosing pre rinse the tip with the liquid by filling and emptying the tip three to five times to improve accuracy and precision 6 2 Test conditions and equi
28. ter claws 44 45 and pull the MCP adapter 46 away Remove the MCP spring base 43 by pulling upwards 6 Remove the two screws 19 and pull the tip ejector 23 into the down position as shown in the picture on page 24 7 While the tip ejector 23 is in the down position open its upper end slightly on both sides and pull downwards Remove the fastening spring 22 Remove the ejector link 24 Press the piston bar 16 beam into the down position Finally unfasten the four screws 20 21 and the tip ejector The ejector slide 25 and two springs 26 can be removed for maintenance This does not belong to the regular long term service however 8 Disengage the housing bottom 17 and piston bar 16 from each other You can disengage the tip cone assembly 42 by pulling it away from the piston assembly 31 Disengage the piston assembly by pulling it out sideways from the piston bar as shown in the picture on page 24 Remove the piston spring 33 7 2 4 Service instructions for multichannel pipette tip cones cylinders Volume 1 10 pl Disengage the tip cone cover 32 from the tip cone A Picture 13 by easing it off with a flat head screwdriver The tip cone cover and the interior parts of the tip cone the second support 35 the O ring 37 the sealing casing 38 the dash spring 39 the spring support 40 and the O ring 41 can all be removed in order by using the piston assembly 31 as shown in the picture If some parts remain in the tip cone they can be removed by t
29. the O ring e O ring damaged Change the O ring Inaccurate e ncorrect operation gt Follow the instructions carefully dispensing e ip incorrectly attached gt Attach firmly e Calibration altered caused by Recalibrate according to the misuse for example instructions e Wrong tip Use the correct tip e Unsuitable calibration a list of Readjust the pipette factors is presented in Section 4 1 Calibration and adjustment e ip cone part 11 is loose Tighten the tip cone firmly to the handle e Pipette not serviced Perform pipette service not ejecting e fitting not properly attached gt Tighten the tip fitting e Uneven tip ejecting with the D Tighten the tip fitting with the multichannel tool or replace the tip fitting set Piston jammed Pipette has been unused for a long Remove the tip if attached period Grease is removed while pipetting a highly volatile solvent repeatedly Press the plunger to the second stop and release several times to re apply the grease Value of the conversion factor Z ul mg as a function of temperature and pressure water Temperature C 80 1 0017 Do 1 0018 16 0 1 0019 16 5 1 0020 17 0 1 0021 1 0022 18 0 1 0022 03 1 0023 190 1 0024 185 1 0025 20 0 1 0026 2085 1 0027 210 1 0028 2109 1 0030 221 1 0031 2200 1 0032 230 1 0033 2308 1 0034 24 0 1 0035 24 5 1 0037 2010 1 0038 279 1 0039 26 0 1 0040 2088 1 0042 27 0 1 0043 27 5 1 0045
30. urning the tip cone upside down and tapping it 32 S N A 30 40 41 Picture 13 Volumes 5 50 pl 10 100 pl and 30 300 Disengage the tip cone cover 32 from the tip cone B Picture 13 by easing it off with a flat head screwdriver The tip cone cover and the interior parts of the tip cone the O ring spring 34 the supporter ring 35 and the O ring 36 5 50yl 10 100yl also O ring 37 can all be removed in order by using the piston assembly 31 as shown in the picture If some parts remain in the tip cone they can be removed by turning the tip cone upside down and tapping it Picture 14 7 2 5 Cleaning and reassembling multichannel pipettes See the cleaning instructions in Section 7 2 2 Single channel pipettes Check the volume specific reassembling of the piston assemblies starting on page 24 The pipette is assembled in the reverse order to disassembling Note that the ejector link 24 must be in the down position simultaneously with the tip ejector 23 as shown in the picture on page 24 Clean and lubricate the holder spring 13 before assembling NOTE To ensure even performance between all channels in a multichannel pipette all tip cones have to be changed at the same time if any of them needs to be changed Don t mix tip cones of different packages because one bag contains a matched set of tip cones Place aligning studs to the same side when assembling the module Picture 1 Picture 15
Download Pdf Manuals
Related Search