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UNICORN Version 4.0 Administration and Technical Manual

1. OutletFractions Enables fraction collection in subse How Many quent outlets from the OutletValve 0 7 Fractionation starts at selected outlet Volume If the number of programmed frac 5 100000 ml tions exceeds the number of outlets Start at the fractionation will resume in posi F2 F8 tion F3 After completing the last frac tion the position selected before executing OutletFractions instruction will be executed Due to the rather long time to turn the valve Volume lt Sml is not allowed C 1 5 Watch Instruction Description Parameters Watch UV1 Sets a conditional watch on the speci Test Watch Cond fied monitor signal causing a branch Value Watch_Conc to a block on the next occasion when Action Watch_AD900 the condition is met Only one watch Watch_pH may be active for a signal at any one Watch Pressure time The watch is deactivated if the Watch Samp Press watch condition is met Watch Samp Flow Watch AirSensor1 Watch AirSensor2 Watch AirSensor3 Watch AuxIn1 Watch AuxIn2 Watch_AuxIn3 Watch AuxIn4 Watch Off Cancels a watch Signal e p107 Strategy for AKTAexplorer 100 mx2 lt lt o 1 6 Hold until Instruction Description Parameters Hold until Hold until is a conditional instruc tion which may be used instead of Watch The method is put on Hold until the specified condition signal test value is met OR until the speci fied ti
2. ii 8 2 3 1 TCP IP Windows NT 2000 domain 8 2 3 2 IPX SPX Novell server 0 13 3 Installation 3 1 Installation summary i 19 3 2 System requirements cee eee 19 3 3 Hardware installation e eee eee 19 3 4 Software installation eee 25 3 4 1 Installing UNICORN for the first time 25 3 4 2 Installing selected software components after the initial installation o 39 3 5 Managing system connections 40 3 5 1 Establishing a connection LL 40 3 5 2 Connection MOdESs Li 41 3 5 3 Leaving and locking a system 42 3 5 4 Disconnecting a System 43 3 5 5 Network considerations 0 000 44 3 6 Calibrating monitors cee eee 44 3 6 1 Calibrating monitors for AKTAdesign systems 46 4 Administration 4 1 System definitions i 4 1 1 Defining new systems 4 1 2 Editing system definitions 4 1 3 Deleting system definitions 4 1 4 Deleting strategy definitions a 4 2 User administration 2 0 ccc eee ee ee 4 2 1 User access SfOUPS iL 4 2 2 Access group examples ee 4 2 3 Creating new users LL 4 2 4 Assigning Users Properties 0a 4 2 5 Changing user passwords LL 4 2 6 Changing user attributes 00 00 4 2 7 Viewing and changing user definitions 4 2 8 Deleting USers a 4 2 9 Deleting folders 0 4 2 10 Printing user setup info
3. Cancel Help Fig 5 1 Instructions dialogue in System Control The illustration shows the Alarms group of settings ep 5 250 System settings Note an 18 1150 11 e p76 2 The instructions are classified into Instructions groups including Alarms Special Monitors and Curves To view the parameters and parameter settings for a particular group click on the appropriate radio button 3 The instructions for the selected instruction group are listed Beneath each instruction is listed the parameters and the current setting 4 To change the setting for a parameter select a parameter from the list and change the associated parameter setting The parameter is updated in the list 5 To restore a setting to its default setting as defined in the system strategy select the setting and click on Set Selected Parameter to Strategy Default Value Only the selected parameters that are marked in the instructions field will be returned to their default values 6 When all required changes have been made click on OK Choosing Cancel will cancel all changes made since the dialogue was last opened 5 1 Alarms Alarms define upper and lower alarm and warning limits for process monitor signals e If the signal exceeds the Alarm limits a alarm sounds and an alarm message is displayed and the process is paused i e method execution is suspended and all pumps are stopped The situation must be acknowledged and corrected
4. Iv Calibration Renew log file Renew every 7 day Set NG Bun Hours Open Backup Print Export Close Help Fig 4 15 System audit trails Run End Manual Displays times for run start and completion and for manual operation Alarms Warnings Displays alarms and warnings defined in the strategy for the system Errors Displays system errors Calibration Displays monitor calibration operations e p67 4 Administration an 18 1150 11 e p68 Viewing older audit trails When you choose Administration Audit trail the audit trail dialogue displays the current audit trail To view an older audit trail click on Open in the audit trail window and choose the file to open The drive is automatically selected according to the type of audit trail file If you have saved log files on diskette these drives can be selected from the pull down list Files are named by date and serial number Choose Current log file to return to the current audit trail after viewing older files Open log file x Drive Log drive 7 Fig 4 16 Open Log File dialogue Printing audit trails Click on Print in the audit trail dialogue menu prints the audit trail file as currently displayed in the window 4 3 2 Renewing audit trail files The audit trail file is renewed at regular intervals between 1 and 30 days To set the interval choose Administration Audit Trail from the Main Menu Enter the required interval in the Renew every field and click
5. Power user Group Administrator User Process operator C Method editor Guest 5 O Evaluation Process supervisor a Group 7 V User setup Levels Group 8 M Audit trail Z System setup Group 9 O Delete move Home only Group 10 M Delete move V Copy file s O Confirm Sign C Unlock locked system C Run methods O Manual interaction V System settings O Edit MethodQueue O Run MethodQueue V Edit global list s V Maintenance V Quit program O Strategy debug Cancel Help Fig 4 6 Suggested authorisation profile for the system administrator The administrator has special responsibility for maintaining user system and audit information and for file management in the PC folder structure backup routines etc The administrator may not edit or run methods or MethodQueues issue manual instructions calibrate monitors or change the system configuration Development staff Users need to be able to edit and run methods and Method Queues issue manual instructions configure system parameters calibrate monitors and evaluate data They may also copy move or delete files Process supervisors Supervisors may pause a method and issue manual instructions as well as start methods and MethodQueues Supervisors are also allowed to calibrate monitors configure system parameters unlock running processes evaluate run data and quit UNICORN e p57 4 Administration Caution an 18 1150 11 e p58 Process
6. 20 Standard C 1 3 Alarms amp Monitors Instruction Description Parameters Wavelength Sets wavelength s for the UV UVI monitor The range is 190 700 nm 190 700 nm for UV1 and OFF 190 700 nm for UV2 UV2 and UV3 OFF 0 190 700 nm UV3 OFF 0 190 700 nm AutoZeroUV Sets the relative AU to zero AutoZeroAD900 Sets the relative AD900 signalto zero AveragingTime UV Filters the noise in the UV signal AvTimeUV Averaging time is the time interval 0 01 used for calculating the moving 0 02 average of the absorbance signal 0 04 A long averaging time will smooth 0 08 out noise efficiently but will also 0 16 distort the peaks The averaging 0 32 time should not be less than 1 28s 0 64 when more than one wavelength is 1 28 monitored A column specific 2 56 averaging time is set automatically 5 12 when a column and a template are selected AD900Filter Sets the filter parameters for Type AD900 signal Average Median Min Max Length 1 20 e p99 Strategy for AKTAexplorer 100 Alarm UV1 Sets the alarm limits for UV1 sig nal An alarm will set the system in Pause Mode Disabled Ena bled HighAlarm 6000 0000 to 6000 0000 mAU LowAlarm 6000 0000 to 6000 0000 mAU Alarm_Cond Sets the alarm limits for the con ductivity signal An alarm will set the system to Pause Mode Disabled Ena bled HighAlarm 0 999 mS cm LowAlarm 0 9
7. After the signal has activated a warning the warning will not be repeated as long as the signal remains within a window defined by the hysteresis setting above and below the warning limit This prevents repeated warnings from noisy or oscillating signals close to the warning boundary Hysteresis is only relevant for warnings since an alarm puts the system in Pause at the first alarm 5 2 Curves Curve settings determine which monitor signals will be stored as curves in the result file Check that Store is set to ON for all signals that are to be stored e p77 System settings r Instructions UWI Parameters C Alarms ping Time between samples 0 100 s C OFF 9 DN CT Specials UV Store ON Time between samples C P Time between samples 0 100 s po 4 Monitors ie 0 100 js Store ON Time between samples 0 100 s Conc Store ON Curves Time hahusen samnles 1 NNN s fai Set Selected Parameter To Strategy Default Value cri He Fig 5 3 Curves instructions in System Control Settings in the Curves group include Store Time between samples Determines whether the curve data is stored in UNICORN The Time between samples determines the frequency with which curve data is recorded in UNICORN this does not affect the reading frequency of the monitor itself Default values are the shortest possible time between samples Caution If a curve is set to Store OFF data from the monitor conce
8. Delta_Base 0 6000 mAU WatchPar_Cond The Delta_Peak setting affects only the Less_than_or_valley and Peak_max conditions when using the Watch instruction see UNI CORN user manual As a general guideline set the value to 2 3 times the noise level and 5 10 of the smallest expected peak height Delta_Base setting affects only the Stable_baseline condition For this condition to be activated the sig nal may not vary by more than the Delta_Base value up or down over the time interval specified in the Stable_baseline condition for the Watch instruction Delta_Peak 0 999 mS cm Delta_Base 0 999 mS cm WatchPar Conc Strategy for AKTAexplorer 100 The Delta Peak setting affects only the Less than or valley and Peak max conditions when using the watch instruction see UNI CORN user manual As a general guideline set the value to 2 3 times the noise level and 5 10 of the smallest expected peak heigth Delta_Base setting affects only the Stable_baseline condition For this condition to be activated the sig nal may not vary by more than the Delta_Base value up or down over the time interval specified in the Stable_baseline condition for the Watch instruction Delta_Peak 0 100 B Delta_Base 0 100 B WatchPar AD900 WatchPar pH The Delta Peak setting affects only the Less than or valley and Peak max conditions when using the Watch instruction see UNI CORN user manual Delt
9. For monitors supplied by manufacturers other than Amersham Biosciences and for some Amersham Biosciences monitors calibration in UNICORN should be performed each time the monitor itself is calibrated For most monitors supplied by Amersham Biosciences calibration from UNICORN also performs a true calibration of the monitor adjusting the signal response level in the monitor Calibration may use one point two point or continuous measurement depending on the type of monitor and system One point calibrations are based on measurements taken at one reference point The monitor will be calibrated based on an assumed linear response between the reference point and zero Two point calibrations are based on measurements at two reference points The monitor will be calibrated assuming a linear response between the two reference values It is important that monitors are calibrated in the measuring range for which they will be used particularly when the response is not linear over the whole operating range of the monitor see Figure below calibrated VA response su actual actual FA working range response response p calibrated po response PA Good calibration Poor calibration Fig 3 21 For a monitor with a non linear response make sure the reference points are within a linear working range Continuous calibration is based on a value accumulated during a given time This kind of calibration applies to the sample
10. No Yes vial and the sample is quantitatively AirSegment transported into the loop with trans NoAir Air port liquid After every injection the needle is rinsed and if WashExt is programmed the outside of the needle is washed with WashVol settings InjectionValve Sets the position of Injection Valve Position The Injection Valve is switched to Load Inject position 1 with parameter load to Waste position 2 with parameter inject and to position 3 with parameter waste The instruction gives an injection mark when the injection valve switches to Inject position ColumnPostion Selects the position for column Position ColumnPosition switches the two col Position1Bypass umn valves simultaneously Usually Position1Bypass corresponds to a tub ing connected to position 1 on both valves Position2 corresponds to a col umn connected to position 2 on both valves etc Position 2 7 e p97 Strategy for AKTAexplorer 100 OutletValve Selects the position for Outlet valve Position Feed switches outlet valve one step Feed WasteF1 forward from its actual position Feed FracF2 F3 F4 from positions F8 and WasteF1 will 8 F5 F6 F7 F8 switch the valve to position F3 Out letValve gives fraction marks BufferValveAl Selects the position for Buffer Valve Position A1 Position A11 corresponds to posi A11 A18 tion 1 on the Buffer Valve A1 Posi tion A12 corresponds to
11. This component includes the UNICORN E application files fessi Space Required 40200 K Space Available 1110376 K pa Fig 3 6 Select Components dialogue Stand alone installation e Check all components Network installation e Program Files must be checked on all stations both local and remote e User Information contains global files for the UNICORN software procedures BufferPrep recipes report formats column files and the users file Click on the Change button to select sub components to install These files need only be installed once in a network For all following installations to the same network these files will be already be present on the server an 18 1150 11 e p28 Installation EB Note The users file should be checked for the first installation only If the users file is checked during later installation on other computers in the network any users already defined will be deleted and only the default user will be available e Strategy and Template files contain a wizard and should be checked for each new strategy to be installed usually when a new system is installed on a local computer Each strategy needs only to be installed once since they are stored on the server e System should be checked on all stations where a system is connected but not on demo or remote stations e Manuals should be checked if you want to install the user manual in pdf format to the target computer If
12. and the New User item In the dialogue that is displayed enter user name maximum 20 characters and must be unique within the domain description and password Click on the Groups button In the dialogue that is displayed select the newly created group of UNICORN users and click on the lt Add button Finally click on the OK button in the Group membership dialogue Click on the OK button in the New User dialogue and the user is created When all users have been created click on Close in the New User dialogue Sharing a folder This is performed by the network administrator 1 6 Log on to the Windows NT 2000 computer designated to be the UNICORN file server with administrative rights Start Windows Explorer with Start Programs Windows Explorer Open the shortcut menu for the folder that will serve as the UNICORN server disk Select Sharing In the dialogue that is displayed click on the Shared As radio button and enter a suitable share name e g UNICORN This name will be used when connecting to this folder Set up the permissions for the shared folder by clicking the Permissions button Select Everyone in the list box and click on the Remove button Click on the Add button and in the dialogue that is displayed make sure that the domain is selected in the top drop down list Select the newly created user group in the list and click on the Add button and finally select Full Control in the bottom drop down list and
13. digital out signal 0 or 1 is set in sys tem settings Check auxiliary equip ment for correct default value C 3 3 Monitors Instruction Description Parameters Wavelength Sets wavelength s for the UV moni UV1 tor The range is 190 700 nm for 190 700 nm UV1 and OFF 190 700 nm for UV2 OFF 0 UV2 and UV3 190 700 nm UV3 OFF 0 190 700 nm AveragingTime UV Filters the noise in the UV signal AvTimeUV Averaging time is the time interval 0 01 used for calculating the moving 0 02 average of the absorbance signal A 0 04 long averaging time will smooth out 0 08 noise efficiently but will also distort 0 16 the peaks The averaging time 0 32 should not be less than 1 28s when 0 64 more than one wavelength is moni 1 28 tored A column specific averaging 2 56 time is set automatically when a col 5 12 umn and a template are selected SetCondScale 0 Sets the 0 scale of the relative CondValue conductivity signal The relative 0 999 9 mS cm conductivity is displayed as a per centage of bufferB in run data Cond and as a curve Cond SetCondScale Sets the 100 scale of the relative CondValue 100 conductivity signal The relative 0 999 9 mS cm conductivity is displayed as a per centage of bufferB in run data Cond and as a curve Cond e p115 Strategy for AKTAexplorer an 18 1150 11 e p116 100 CondTempComp Relates conductivity to temperature The com
14. 18 1150 11 e p40 Installation EB To connect to a system from a remote station in a network installation the local station i e the computer physically connected to the chromatography system must be logged on to the network The UNICORN drivers must be running on the local station although the UNICORN program does not need to be running A local station can be used to control the chromatography systems directly connected to the PC without logging on to the network Files stored on network drives will of course not be accessible This mode of working places UNICORN into an error state and you should not ideally work in this state since global files such as the user settings file musers30 mpm etc are stored on the network Any changes made to these files while not logged on will apply locally and will be lost the next time you log on to the network to use UNICORN For runs performed in this stand alone mode where the result file is directed to a network drive the results will be saved in the Failed folder on the local station Several simultaneous connections can be established to one system but only one may be in control mode i e able to actively control the system The other connections are in view mode and can monitor the system activity but cannot issue commands 3 5 2 Connection modes The possible connection states of a System Control module are indicated by the status text on the status bar as summarised below Conne
15. 3593 Fax 1 800 329 3593 BioPilot BioProcess UNICORN and Akta are trademarks of Amersham Biosciences Limited or its subsidiaries Amersham and Amersham Biosciences are trademarks of Amersham plc Windows Windows NT and Windows 2000 are trademarks of the Microsoft Corporation Novell is a trademark of the Oracle Corporation Amersham Biosciences AB Bj rkgatan 30 SE 751 84 Uppsala Sweden Amersham Biosciences UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA England Amersham Biosciences Inc 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 USA Amersham Biosciences Europe GmbH Munzinger Strasse 9 D 79111 Freiburg Germany Amersham Biosciences K K Sanken Building 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan All goods and services are sold subject to the terms and conditions of sale of the company within the Amersham Biosciences group that supplies them A copy of these terms and conditions is available on request Amersham Biosciences AB 2001 All rights reserved CONTENTS 1 Security features 151 ACCOSS SEGUINY oarnein pa de a 1 1 2 Connection security ees 1 1 3 Data security iii 2 1 3 1 Network communication failure aaa aaea 2 1 3 2 ikocalistationirailure crd ii a anan 3 1 4 Security recommendations for control stations 3 2 Network setup 2 1 Introduction oirrese teiaa a 5 2 2 Requirements lira Rie eeeh 7 2 3 Installation guide
16. A Technical specifications an 18 1150 11 p88 Feedback tuning GE B Feedback tuning Some systems allow you to perform PID feedback tuning of the pump flow rate using the System Tune command in System Control Other systems allow you to perform tuning with the insertion of a tune instruction in the method Other systems such as the AKTAdesign systems do not use tuning at all This appendix describes the principles of PID feedback tuning of pump flow rate to available systems UNICORN process control software provides a graphical interface for feedback tuning Feedback control is aimed at eliminating discrepancies between the actual value and the requested value in this case flow rate BioProcess System uses the measured flow rate to control overall pump speed and the measured conductivity to control the relative speeds of pumps A and B in gradient formation PID tuning uses three parameters to determine feedback control e The P parameter reduces the effect of an error but does not completely eliminate it A simple P regulator results in a stable discrepancy between actual and requested flow rate a stationary error e The I parameter eliminates the stationary error but results in a slight instability leading to oscillations in the actual flow I can have values between 0 and infinity where smaller values have greater effect and a value of infinity has no effect The value infinity is set as 9999 in UNICORN e In certa
17. After installation the following operations should be performed in UNICORN by the administrator before other users can use the program 1 Set up system definitions for the chromatography systems see System definitions on page 49 2 Define new users with home folders and access groups and access 8 p profiles see User administration on page 53 Note These operations can be performed on any station in a network installation It is however important that the administrator is logged on to the network on the station being used so that the changes will apply globally throughout the network 4 1 System definitions System definitions set up the chromatography systems which are connected directly to the local computer in UNICORN installation This must be done for each new system installed In a network installation these definitions must be set up for each local computer in e p49 4 Administration an 18 1150 11 e p50 the network but the actual set up operations can be performed on any computer Rights of access to system are controlled at the user administration level see User administration on page 53 To manage system definitions choose Administration System setup in the Main Menu To use this menu command you must have Audit trail System setup authorisation see above System Setup x Systems Close New Edit Delete F Help Fig 4 1 System Setup dialog
18. Instruction Description Parameters Autosampler AutosamplerControl consists of dif Command Control ferent autosampler commands Initial InitialWash Wash performs a complete wash of RotateTray needle and syringe RotateTray is used SyringeHome when tray segments is placed on the SyringeEnd tray and when new vials are loaded NeedleHome onto the tray SyringeHome and NeedleFront C 2 3 Alarms amp Monitors This group contains the same instructions as the Alarms amp Mon group in method instructions Strategy for AKTAexplorer 100 C 2 4 Frac This group contains the same instructions as the Frac group in method instructions with the following additions Instruction Description Parameters Man_Fractionation Starts fraction collection if the frac Rack tion size specified by the FracSize 1 26 parameter is gt 0 Tube change is Frac_order delayed with respect to the set delay 1 4 volume FractionCollector is stopped Tube type with the instruction Fractionation 1 2 Stop FracSize 0 100000 Start_at 0 4 Basel 1 2 Man Peak Fraction Peak_Fractionation enables only Rack ation peaks to be collected The UV monitor 0 26 is used to determine when there is a Frac_order peak to collect PeakSize determines 0 4 max volume to be collected in each Tube_type tube To collect the peak in one tube 1 2 set PeakSize gt peak volume If PeakSize Peak_FracParameters are not set in 0 100000 the method
19. MOonitors a Gawad UA 110 Ac AA AA enh 111 C 3 System settings instructions a 111 Cal Ala IS aaa na Paan ble Dna ALAGANG AG MD Asa ee hala 111 Cazsspeclals zag iii eE pA ade ha NAT naaa ayaan a 112 Ca o Monitors sted BAN AN DA LG ad NA LB Soa dee 115 GB AoCUNES ALY BAAL AN Mat AD ba BOL ka no PA Pe ia Pk 118 Security features CE 1 Security features This chapter considers security features in UNICORN under the topics access security e system connection security e data security e security recommendations for control stations 1 1 Access security Operation of UNICORN is restricted to authorised users defined in the system by the system administrator see User administration on page 53 User access may be protected by a password The minimum password length for users is defined when UNICORN is installed see Software installation on page 25 In installations where access security is important passwords should be enforced and should be changed regularly see Changing user passwords on page 63 Fach user is assigned an access level that defines the operations that the user can perform and the folders the user is allowed to access see User administration on page 53 For system security administrative routines such as user and system definition should be permitted only to the system administrator Installation of UNICORN establishes a default user with full access rights in the system I
20. Password Password required Minimum number of characters E No password required System Control Windows CM so LE wa Fig 3 9 Password and System Control Window Settings dialogue 12 Select the program folder in which you want the UNICORN icon to be placed You can either create a new folder or select one that already exists The folder will be placed in the programs menu under the Start button menu Select Program Folder x Setup will add program icons to the Program Folder listed below You may type a new folder name or select one from the existing Folders list Click Next to continue Program Folders Unicom Existing Folders Administrative Tools Common Adobe Acrobat Intel LANDesk Virus Protect 5 0 Microsoft Developer Network Microsoft Visual C 5 0 lt Back Cancel Fig 3 10 Select Program Folder dialogue an 18 1150 11 e p32 Installation ER 13 At this point the setup program is ready to start copy the files A dialogue will display all the selections that have been made If the settings are correct click on the Next button to start copying the files If you want to make any changes you can click on the Back button Start Copying Files x Setup has enough information to start copying the program files If you want to review or change any settings click Back If you are satisfied with the settings click Next to begin copying fi
21. Stable_baseline condition for the Watch instruction Delta_Peak 0 10 MPa Delta_Base 0 10 MPa WatchPar Sample Pressure WatchPar Sample Flow The Delta Peak setting affects only the Less than or valley and Peak max conditions when using the Watch instruction see UNI CORN user manual Delta Base setting affects only the Stable baseline condition For this condition to be activated the sig nal may not vary by more than the Delta Base value up or down over the time interval specified in the Stable baseline condition in the Watch instruction The Delta Peak setting affects only the Less than or valley and Peak max conditions when using the Watch instruction see UNI CORN user manual Delta Base setting affects only the Stable baseline condition For this condition to be activated the sig nal may not vary by more than the Delta Base value up or down over the time interval specified in the Stable baseline condition in the Watch instruction Delta Peak 0 1 MPa Delta Base 0 1 MPa Delta Peak 0 50 ml min Delta Base 0 50 ml min UVlampOFF Sets the UV lamp OFF This com mand only influences the specific run Strategy for AKTAexplorer 100 C 1 4 Frac Instruction Description Parameters Fractionation Starts fraction collection if the frac Tube_type tion size specified by the FracSize 0 2 parameter is gt 0 Tube change is FracSize delayed with resp
22. access to this function is restricted 2 Enter a password and confirm the password in the Password and Confirm spaces 3 Select a user group from the User drop down list 4 Select a home folder from the Home folder drop down list You may choose any folder from this list even if you created a home folder in the New User dialogue User properties x e Dave David wilson r User e Advanced User name Dave Hom Access 5 1 44 Instructions Full name David Wilson Position Prod Res Password pee Confim fem SS Group Administrator z Home folder pw Administrator Attributes T User disabled Ta Must change password atnevt logan User Attributes T Use large icons TT Show unused variables TT Show details Cancel Help Fig 4 10 The User properties dialogue Administrator Attributes can be set in this dialogue by checking the boxes User disabled and Change password at next logon User Attributes can also be set in this dialogue by checking the boxes Use large icons Show unused variables and Show details Advanced User Properties The advanced password functions are password age and uniqueness account lockout automatic workstation lock and signature To select e pol 4 Administration Note an 18 1150 11 e p62 these properties select a user in the User setup dialogue and click on Edit to access to Users Properties dialogue Click on Advanced in the drop down
23. and enter a name for the workgroup The network administrator should supply this name click on Next to continue Click on Finish Remove the CD ROM disk Select the option to restart your computer now The computer is restarted Log on with administrator rights The Select NetWare Logon dialogue is displayed The network administrator must supply the Default Tree and Context to use Select the Default Tree and Context radio button enter the information from the network administrator and click on OK Since the name and password of the user you logged on as are not a valid account on the Novell server a message will be displayed after a 30 second timeout NetWare Authentication Failure Click on Yes to keep the default tree and context Installing UNICORN 1 2 Start Windows Explorer with Start Programs Windows Explorer Connect the shared folder on the UNICORN file server with the designated drive letter You can do this by selecting Tools Map Network Drive In the dialogue that is displayed select the drive letter in the upper drop down list box 10 11 12 Network setup CH Enter a valid domain user ID in the Connect As field In the Shared folders list at the bottom of the dialogue the network can be seen double click on the Novell server with the shared folder Available shared folders on the selected server are displayed Double click on the shared folder UNICORN will use A dialogue appears w
24. before the process can be restarted e If the signal exceeds the Warning limits a warning message is issued without interrupting the process Alarm and warning messages are displayed on all stations with a connection to the system concerned regardless of the activity currently being performed in UNICORN and regardless of the identity and access rights of the current user Alarms and warnings can however only be acknowledged from the control mode connection If allowed by the system strategy limits for certain monitor signals may also be set locally in a method overriding the global setting as long as the method is in operation This feature allows for instance the pH warning threshold to be set to one value during process operation and another during system cleaning Caution System settings 5 Error messages from the monitors and pumps are reported if the respective Error settings are Enabled Alarms are not active unless the mode is set to Enabled This can be viewed for each instruction in the list The hysteresis setting not available for AKTAdesign systems for a warning determines the extent to which the signal can oscillate around the warning limits without re activating the warning Warning im re WARNING Fig 5 2 The hysteresis not available for KTAdesign systems setting defines the limits within which the signal may oscillate up or down from the threshold without re activating a warning
25. checked A network installation can be either a local station or a remote only system To get a remote only system i e a computer to which no systems are physically connected check Remote only system For a network installation you will also have to select a server disk where the server files are to be located When you perform a network installation the necessary parts of UNICORN software will be copied automatically to the network server disk Note If you perform a stand alone installation and later want to connect the an 18 1150 11 e p30 system to a network you must remove the current installation and install the software with the appropriate settings Installation Setup Type Fig 3 8 Setup Type dialogue 11 Enter the minimum number of characters required for passwords Valid numbers of password characters are 3 15 Alternatively if you do not require password protection select No password required With this setting users can be defined with or without passwords Note In a network installation make sure that you enter the same password settings on each station in the network Choose the number of System Control windows that should be available in the installation maximum 4 ep3l ED Installation Password and System Control Window Settings Set the requirement for minimum number of password characters and the number of system control windows Press the NEXT button to continue m
26. default values in system Start at settings will be used Tube change is 0 4 delayed with respect to the delay vol Base ume To stop PeakFractionation use 1 2 the instruction Peak_FracStop Ignore_LastTube Use the instruction Ignore_LastTube if you want to fractionate after the last tube position set in the method C 3 System settings instructions C 3 1 Alarms This group basically contains the same instructions as the Alarms amp Mon group in method instructions The following instructions are not included however Wavelength AutoZeroUV AutoZeroAD900 AveragingtimeUV AD900Filter SetCondScale0 SetCondScale100 WatchPar UV1 WatchPar_Cond WatchPar_Conc WatchPar_AD900 WatchPar_pH eplll an 18 1150 11 e p112 Strategy for AKTAexplorer 100 WatchPar_Pressure WatchPar_SamplePressure WatchPar_SampleFlow Also one new alarm is added Instruction Description Parameters Wash_Alarm_Press Sets the alarm limit during PumpWash Mode lure land SystemWash An alarm will set Disabled Ena the system to Pause bled HighAlarm 0 10 MPa C 3 2 Specials Instruction Description Parameters Autosampler Sets the hardware related autosampler LoopVol Parameters parameters When replacing the sam 10 1000 ul ple loop or syringe check A 900 User SyringeVol Manual for correct syringe loop and 100 ul 250 ul buffer tubing combinations Flush vol 500 ul 1000
27. file To re install these global files check each sub component If for example the existing user definitions are damaged you can check the Users file to re install the default user Any other users defined in the system including users installed from other stations in a network installation will be deleted However method and result files will not be erased You can regain access to these files by re defining users with appropriate folder access Users are installed on the network server in a network installation System installation Check the System installation option if you are installing a new or an additional system on a stand alone computer or a local station in a network or if you want to change the settings for an existing system Systems are not installed on demo or remote only systems Strategy files Check the Strategy files option to re install system strategies or to install additional strategies Accept the suggested name or enter a new name for the strategy This will not affect any existing method or result files in the system Strategies are installed on the network server in a network installation e p39 DD Installation Check the Template files option to re install template files and wizard or to install template files for a new strategy Normally templates are installed together with strategies If you are not installing a strategy at the same time a dialogue will be shown displaying all strategies that are ins
28. folder Available shared folders on the selected server are displayed Double click on the shared folder UNICORN will use A dialogue appears with a request to give a valid user name and password The user name will be the ID previously entered in the Connect As field Give the password and click on OK Install UNICORN see Installation on page 19 select Network version Re boot the PC and log on as one of the domain users that will run UNICORN Connect the shared folder again this is necessary since each user has his own connected network drives Repeat steps 1 2 3 5 and 6 above and make sure the Reconnect at Logon option is checked Start UNICORN and set up system definitions and user profiles Installation on page 19 2 3 2 IPX SPX Novell server This is an example of how to set up the network so that UNICORN can run across the network when the network protocol is IPX SPX and uses a Novell server e p13 HD Network setup ss The general network organisation is described in the following picture an 18 1150 11 e p14 PC with UNICORN 9 GODO ADDA f Chromatography system m fl 9 CODE 5 PC with UNICORN Network sever Chromatography system GODO falas Chromatography system ODE e DODO PC with UNICORN PC where UNICORN PC with UNICORN is NOT installed is Chromatography sys
29. installation 39 stategy and or template files 35 strategy files after the initial installation 39 system after the initial installation 39 template files after the initial installation 40 user information after the initial installation 39 installing IPX SPX on the computer 15 installing TCP IP on the computer 10 installing UNICORN 16 installing UNICORN on network 8 installing UNICORN NT domain with TCP IP 13 instructions alarms and monitors 99 flowpath 96 manual control 110 miscellaneous 108 pump 93 system setting 111 watch 107 instructions groups 76 instructions manual alarms and monitors 110 flowpath 110 instructions system settings alarms 111 curve 118 monitors 115 specials 112 IPX SPX installing on the computer 15 IRQ setting 20 L leaving system 42 license agreement 27 local station definition 7 failure 3 locking system 42 logoff problem logging off 81 logon problem logging on 79 M managing system connections 40 manual control instructions 110 maximising resolution 87 method problems 81 red instructions 82 method instructions pump 93 modes connection 41 monitors calibrating for AKTAdesign systems 46 calibration procedure 44 system settings instructions 115 e 123 um 18 1150 11 e 124 network communication error 7 communication failure security 2 computer installations 86 control access rights 44 controlling systems from local and remote stations 7 creating home folder 60 creating
30. of UNICORN is installed on all stations in the network 3 4 1 Installing UNICORN for the first time The installation procedure described below assumes that the operating system Windows NT 2000 is correctly installed on your computer Refer to the operating system documentation for details For network installations the network must be correctly set up UNICORN is supplied on a CD ROM Files on the CD ROM are compressed and cannot simply be copied onto the hard disk The installation procedure described below creates the required folder structure on the hard disk and decompresses the files Do not attempt e p25 ED Installation an 18 1150 11 e p26 to decompress the distribution files using any other file decompression utility Follow the procedure below to install UNICORN For a network installation follow this procedure on each computer in the network You can quit the installation at any point by clicking on either the Cancel button or the Exit button If you do this however the installation will be incomplete and the software cannot be used 1 For network installations log on to the network and check that you have access to the server disk and folder where UNICORN network components are to be installed 2 Insert the CD ROM disk into the CD ROM drive 3 Select Run from the Windows taskbar Start button menu 4 Write the command d setup where d is the unit for your CD ROM drive Click on OK 5 The UNICORN s
31. position 2 etc PumpAInlet Sets the position of pump A inlet The Position instruction is not allowed ina method A1 A2 when running BufferPrep gives a warning in BufferPrep mode PumpBiInlet Sets the position of pump B inlet The Position instruction is not allowed ina method B1 B2 when running BufferPrep gives a warning in BufferPrep mode SampleValve Selects the position for the Sample Position Valve S1 58 Valve8 Sets the valve position for valve with Position ID code 8 Feed 0 1 8 Valve9 Sets the valve position for valve with Position ID code 9 Feed 0 1 8 FlowDirection Sets the flow direction for the column Direction The instruction is useful when clean DownFlow ing columns The instruction switches UpFlow the Flow Direction Valve to position 2 with parameter UpFlow and to posi tion 1 with parameter DownFlow The flow is reduced to 0 ml min when the valve switches InjectionMark The instruction gives an injection mark The instruction is useful when the sample is introduced onto the col umn with the system pump an 18 1150 11 e p98 Quantitation Data Strategy for AKTAexplorer 100 The parameters of QuantitationData are sent to evaluation module to be used when updating quantitation table Different levels of standards are used for updating quantitation table and Sample is used for quantitation of sample Type Sample Level 1 Standard to Level
32. possible to e Create or delete users and assign users to user access groups e Change user access groups e Create and manage user s folders e p53 4 Administration an 18 1150 11 e p54 fo Define users and their security attributes m Access groups a Each user is assigned to an access group This group defines access to different functions in UNICORN When changing a group all users Access groups assigned to that group are affected m Users ame Full name Group default default Administrator Stephen Clegg Administrator Fig 4 5 User Setup dialogue 4 2 1 User access groups UNICORN has 10 different access groups that can be named and assigned to differing levels of access to UNICORN To edit an access group select Administration User Setup from the Main Menu In User Setup click on Access groups to view the User Properties dialogue To use this menu command you must have User setup Levels authorisation see below To change the name of a group enter a new name on the bar Access then click on OK If you change the definition of an access group to which users are already assigned the changes will apply to all users in this group At least one access groups must have User setup Levels authorisation UNICORN will not allow you to remove this authorisation from all levels Click on the Print button in the User Setup dialogue to obtain a record of each authorisation level that
33. pump in AKTAdesign systems e p45 DD Installation an 18 1150 11 e p46 3 6 1 Calibrating monitors for AKTAdesign systems To calibrate the monitors for AKTAdesign systems do the following 1 Select System Calibrate to display the Calibration dialogue 2 Select each of the monitors in turn and perform the procedures as described in the dialogue 3 Click on Close once the calibrations have been made Example pH System Control 1 Explorer100 Calibration Calibration procedure Calibrate pH electrode for buffer 1 See Help pH 1 Reference value 1 pana 00140 2 Read value 1 pH ETON IED 3 Reference value 2 eur 00140 a Fn Calibrated electrode slope esa Asymmetty potential at pH7 mV Monitor Measured value Help Fig 3 22 Dialogue for pH calibration Some AKTAdesign systems have a pH monitor to allow on line pH measurements Calibration can be performed with the electrode either within the flow cell or outside If calibrating with the pH electrode sitting in the flow cell 1 Attach the first pH reference solution e g pH 7 0 to Inlet A on the system and the second reference solution e g pH 4 0 to Inlet B 2 In System Control select Manual Pump select the instruction PumpWash Explorer Purifier Basic and then choose Inlet A Click on Execute The pump and inlet tubing will now be filled with the first reference solution 3 When the PumpWash is fini
34. see System definitions on page 49 e If connecting to a system remotely check that the local station physically connected to the system is turned on and that the network is functioning at both the remote and the local station e Check that the limit of eight connections to the system has not been exceeded 6 3 Method and run problems 6 3 1 Cannot Quit or Logoff from UNICORN If you are unable to Quit or Logoff from UNICORN for a connection you may be running a scouting method or a MethodQueue These functions require a control mode connection in order to start subsequent cycles correctly 6 3 2 Monitor signals do not appear in the System Control Curves panel For monitor signals to be displayed in System Control they must be set to STORE ON in system settings Signals for which STORE ON ss set can be chosen from the View Curve contents dialogue box for display in the curves panel 6 3 3 Error message Couldn t create result file If you receive an error message Couldn t create result file Destination path could not be found at the end of a method the local computer was unable to access the folder specified in the result file path This may arise if the specified folder is on the network server and network communication has been lost The result file is saved in the FAILED folder on the local station 6 3 4 The Method System Connection dialogue keeps appear ing If the Method System Connection dialogue keeps appe
35. signal rises above the set MinWidth level value and is ended when the UV 0 01 1500 min signal goes below the set value MinWidth sets the minimum Peac Fractionation time Peak Fractionation continues at least until MinWidth is reached even if the signal is fluctuating AD900Filter Sets the filter parameters for AD900 Type signal Average Median Min Max Length 1 20 Keyboard Set the mode of the user interface of Mode the instruments Open Key Open Instructions can also be set on Locked KeyAnd the instruments KeyLocked Menus DialLocked can be viewed on the instruments by turning the dial KeyAndDialLocked The user inter face of the instruments is locked AuxOut1 Sets signal on digital out 1to Oor 1 Signal Make sure correct default value for 0 1 digital out signal 0 or 1 is set in sys tem settings Check auxiliary equip ment for correct default value AuxOut2 Sets signal on digital out 2 to Oor 1 Signal Make sure correct default value for 0 1 digital out signal 0 or 1 is set in sys tem settings Check auxiliary equip ment for correct default value AuxOut3 Sets signal on digital out 3 to Oor 1 Signal Make sure correct default value for 0 1 digital out signal 0 or 1 is set in sys tem settings Check auxiliary equip ment for correct default value Strategy for AKTAexplorer 100 AuxOut4 Sets signal on digitalout4to0or1 Signal Make sure correct default value for 0 1
36. steps to generate your own report There are two ways of accessing the Report Generator Wizard e you can generate a report in the Main Menu by selecting the menu command Administration Create System Report e when an error message appears in System Control you can activate the report generator by clicking on the Report button in the message dialogue 4 4 1 Generating a report from the Main Menu Generate Report Wizard This first dialogue gives an introduction to the report generator e p69 4 Administration an 18 1150 11 e p70 Generate Report Wizard This wizard will help you quickly generate a report on the UNICORN software To begin the report generation click Next Cancel Heb Systems This dialogue is displayed only if you have accessed the wizard from the Main Menu This displays a list of the available systems for the logged on user In order to proceed further a system must be selected for which the report is to be generated r Select system to make a report on Basic10 Print W Cancel Help Administration CU Description You are required to enter a short description of the problem the circumstances under which the problem occurs and the consequences caused by the problem Description Reproducibility You are required to give information concerning the reproducibility of the problem If the problem is reproducible then you can describe how to bring ab
37. the connection becomes a view mode connection After leaving and locking scouting or MethodQueue runs it is not possible to establish a control mode connection from another computer PI Installation Leave Control of Explorer100 Ea Leave system Unlocked Locked Username default Password Cancel Help Fig 3 20 Leave Control of system dialogue You may leave the system unlocked or locked e Unlocked leaves the system unlocked Any other user may establish a control mode connection to the system Use this option if you do not intend to use the system in the near future e Locked locks the system with the password specified in the dialogue A control mode connection can only be established by providing the correct password Note that this password is independent of the user s logon password A locked system can also be unlocked with the logon password for a user with Unlock locked systems authorisation This authorisation should be restricted to a small number of users to prevent indiscriminate unlocking of locked systems 3 5 4 Disconnecting a system To disconnect a chromatography system from a System Control module click on the Disconnect from system icon or choose System Disconnect If you are disconnecting a control mode connection you will be asked if you wish to leave control of the system under a password protected lock Logging off or quitting UNICORN automatically disconnec
38. the local station Results are saved on the local station with the original result file name in the folder DRIVE Unicorn Local Fil Failed from which they can be retrieved after the run is complete To retrieve results from the Failed folder 1 Start UNICORN if it is not already started on the local station where the separation process was run 2 Log on as a user with access to the Failed folder 3 You can now move the result file to a suitable location on the network server when network communication is re established so that it is accessible from remote stations Result files are saved directly under the Failed folder and are identified by result file name Files with the same result file name base are distinguished by an incremental serial number in the same way as result files in any other folder Access to the Failed folder The system administrator may choose one of two policies concerning access to the Failed folder e Grant access to the Failed folder to all users so that each user can retrieve his or her own result files This places the responsibility for retrieving result files and deleting old files from the Failed folder on the individual users Note that with this policy any user will be able to examine copy move and delete the other users results in the Failed folder This policy has the advantage that the Failed folder can conveniently be used to temporarily store methods and results from runs performed from
39. user groups and users NT domain with TCP IP 9 creating user groups and users NT workstations connected to a Novell server 14 data transfer 86 example 9 installation 86 installation of UNICORN 8 maintenance 6 minimum requirements for running UNICORN 7 requirements 85 security implications 6 setup 5 setup introduction 5 sharing a folder NT domain with TCP IP 10 sharing a folder NT workstations connected to a Novell server 14 supported network cards 85 UNICORN in the network environment 5 0 oligo system type selection 50 P parameter setting changing 76 restoring default value 76 viewing 76 password 31 63 pH calibrating the pH sensor 46 PID feedback tuning 89 tuning parameters 89 print audit trails 68 can not print screen on printer 83 user setup information 65 pump instructions 93 Q quit problem quitting UNICORN 81 R remote station 7 report generate 73 generate from System control 73 generating from the main menu 69 report generator AKTA hardware information 72 attachments 72 integrity check 73 problem description 71 reproducibility information 71 systems list 70 Wizard 69 resolution and sampling frequency 86 maximising 87 run problems 81 S sampling frequency 86 screen problem with fit of method editor window on screen 82 security access 1 connection 1 data 2 features overview 1 in network 6 recommendations for control stations 3 selecting system components 51 setti
40. with the latest version of your driver and enter the path to it in the dialogue In the Network dialogue enter the Services tab and click on the Add button In the dialogue that is displayed select Server and click on the OK button Click on the Add button again In the dialogue that is displayed select Workstation and click on the OK button In the Network dialogue enter the Protocols tab and click on the Add button In the dialogue that is displayed select TCP IP Protocol and click on the OK button 12 Network setup Gi In the Network dialogue click on the OK button When the window is writing this new data to the hard disk the configuration dialogues for the TCP IP protocol will be displayed Information specific to the network must be entered This information should be supplied by the network administrator Installing UNICORN 1 di 10 11 Start Windows Explorer with Start Programs Windows Explorer Connect the shared folder on the UNICORN file server with the designated drive letter You can do this by selecting Tools Map Network Drive In the dialogue that is displayed select the drive letter in the upper drop down list box If the current user ID does not have permissions to the shared UNICORN folder on the server enter a valid domain user ID in the Connect As field In the Shared folders list at the bottom of the dialogue the network can be seen double click on the server with the shared
41. you create The authorisation items are e Method Editor Required for using the Method Editor for creating and editing methods Chapters 4 and 5 of the User Manual Administration ci Evaluation Required for using the evaluation module for processing result data Chapters 9 and 10 of the User Manual User setup Levels Required for defining and changing access levels and user Caution We recommend that only one user in an installation or network is assigned this access Audit trail System setup Required for examining the audit trail and for defining connected systems see Audit trails on page 66 Caution We recommend that only one user in an installation or network is assigned this access Delete move Home only Required for deleting and moving files and folders within the user s home folder Does not authorise these operations on other folders Delete move Required for deleting and moving files and folders outside the user s home folder Also authorises these operations within the home folder Copy file s Required for copying files The user must have access to both the source and target folders for moving or copying between folders Confirm Sign Required for authorised confirmation of answers to start protocol questions and signing of methods and result files Unlock locked system Permits a user to unlock locked systems by providing the user s own logon passwords locked systems can normally only
42. 11 07 18 Connection change User default at computer UPPPCOS connected to system Wizard F Logon Logolf 2001 04 09 11 07 19 Connection change User default at computer UPPPCOB connected to system Explorer 00 Define system MethodQueue I Maintenance Renew log file Renew every 7 day mg Set Now Bun Hour Open Backup Print Export Close Help Fig 4 14 Global audit trails User Displays all user creation deletion and re definition operations Backup Displays backup operations for global audit trail files Log on off Displays all logon and logoff attempts with the name of the user logging on or off including failed logon attempts Administration ci Define system Displays all system definition deletion and redefinition events MethodQueue Displays MethodQueue start operations System audit trails Select the system for which the audit trail is to be displayed from the drop down list and check the items you want to view All items are recorded in the audit trail the check boxes in the System field only control which items are displayed System audit trail files are saved on the local station to which the system is physically connected and may be examined from the local station without logging on to the network System audit trail files can be viewed from any computer in a network installation Audit Trail x C Global System System Explorer100 x I Run End Manual IV Alarms Wamings M Enors
43. 99 mS cm Alarm_AD900 Sets the limits for AD900 signal An alarm will set the system to pause Mode Disabled Ena bled HighAlarm 5000 to 5000 mV LowAlarm 5000 to 5000 mV Alarm_pH Sets the alarm limits for the pH signal An alarm will set the sys tem to Pause Mode Disabled Ena bled HighAlarm 0 14 pH LowAlarm 0 14 pH Alarm_Pressure Sets the alarm limits for pressure An alarm PressureHigh Alarm PressureLowAlarm will set the sys tem to Pause Mode Disabled Ena bled HighAlarm 0 10 MPa LowAlarm 0 5 MPa an 18 1150 11 e p100 Alarm_Sample Strategy for AKTAexplorer 100 Sets the alarm limits for sample Mode Pressure pressure An alarm SamplePressu Disabled Ena reHighAlarm SamplePressure bled LowAlarm will set the system to HighAlarm Pause 0 1 MPa LowAlarm 0 1 MPa Alarm_Auto Sets the temperature limits for the Mode Sampler autosampler An alarm will set the Disabled Ena system to Pause bled HighAlarm 4 40 C LowAlarm 4 40 C Warning Auto Sets the temperature limits for the Mode Sampler autosampler A warning will be displayed on the screen but will Disabled Ena bled not set the system to pause HighWarn 4 40 C LowWarn 4 40 C Alarm_AirSensor1 Sets the alarm for AirSensor1 An Mode alarm will set the system in Pause if air is detected in air sensor flow cell 1 Di
44. CORN usage and system activity for the system administrator The audit trail may be viewed in global mode all systems in the installation or system mode one chosen system 4 3 1 Examining audit trails In the audit trail window select whether you wish to view the Global or System trail Global audit trails Check the items you want to display in the Global field All items are recorded in the audit trail the check boxes in the Global field only control which items are displayed Global audit trail files are saved on the server disk in a network installation and a network connection is required to examine global audit trails 2001 04 03 17 10 50 Logoff default C System 2001 04 03 17 10 52 Connection change User at computer UPPPCOE disconnected from system Wizard 2001 04 03 17 10 53 Connection change User at computer UPPPCOE disconnected from system Explorer 00 Global 2001 04 06 10 14 18 Logon default 2001 04 06 10 14 21 Connection change User default at computer UPPPCOS connected to system Wizard 2001 04 06 10 14 21 Connection change User default at computer UPPPCO6 connected to system Explorer100 gi 2001 04 06 14 14 51 Logoff default 2001 04 06 14 14 57 Connection change User at computer UPPPC06 disconnected from system Wizard 2001 04 06 14 14 58 Connection change User at computer UPPPCOS disconnected from system Explorer 00 M Backup 2001 04 09 11 07 14 Logon default 2001 04 09
45. FF OFF OFF 170 offset OFF OFF OFF 180 offset ON ON ON 190 offset OFF ON ON LAO offset ON OFF ON 1B0 offset OFF OFF ON 1C0 offset ON ON OFF 1DO offset OFF ON OFF 1E0 offset ON OFF OFF 220 offset ON OFF ON 230 offset OFF OFF ON OFF 240 offset ON ON OFF OFF 250 offset OFF ON OFF OFF 260 offset ON OFF OFF OFF 280 offset ON ON ON OFF 290 offset OFF ON ON OFF DAO offset ON OFF ON OFF 300 offset ON ON ON OFF e p23 ww Installation an 18 1150 11 e p24 310 offset OFF ON ON OFF OFF 320 offset ON OFF ON OFF OFF 330 offset 340 offset OFF ON OFF ON ON OFF OFF OFF OFF OFF 350 offset OFF OFF OFF OFF BEO offset ON OFF OFF OFF OFF UNICORN Control Board AT BioPilot systems Alternative IRQs for this card are 3 5 10 and 11 The manufacturing default setting is IRQ10 The jumpers of the multinet card correspond to IRQs as follows Alternative addresses for this card are shown in the table The value that should be entered as a
46. Q nana chromatography software UNICORN Version 4 0 Administration and Technical Manual Amersham wn 18 1150 11 e Y Biosciences UNICORN 4 0 Administration and Technical Manual 18 1150 11 Edition AA 2001 04 to order e Asia Pacific Tel 852 2811 8693 Fax 852 2811 5251 e Australasia Tel 61 2 9894 5152 Fax 61 2 9899 7511 e Austria Tel 49 761 4093 201 Fax 49 761 4903 405 e Belgium Tel 0800 73 888 Fax 03 272 1637 e Canada Tel 1 800 463 5800 Fax 1 800 567 1008 e Central East South East Europe Tel 43 1 982 3826 Fax 43 1 985 8327 e Denmark Tel 45 16 2400 Fax 45 16 2424 e Finland amp Baltics Tel 358 09 512 3940 Fax 358 09 512 1710 e France Tel 0169 35 67 00 Fax 0169 41 9677 e Germany Tel 0761 4903 201 Fax 0761 4903 405 e Italy Tel 02 27322 1 Fax 02 27302 212 e Japan Tel 81 3 5331 9336 Fax 81 3 5331 9370 e Latin America Tel 55 11 3667 5700 Fax 55 11 3667 87 99 e Middle East and Africa Tel 30 1 96 00 687 Fax 30 1 96 00 693 e Netherlands Tel 0165 580 410 Fax 0165 580 401 e Norway Tel 2318 5800 Fax 2318 6800 e Portugal Tel 01 417 7035 Fax 01 417 3184 e Russian 8 other C 1 S amp N I S Tel 7 095 232 0250 956 1137 Fax 7 095 230 6377 e South East Asia Tel 60 3 8024 2080 Fax 60 3 8024 2090 e Spain Tel 93 594 49 50 Fax 93 594 49 55 e Sweden Tel 018 612 19 00 Fax 018 612 19 10 e Switzerland Tel 01 802 81 50 Fax 01 802 81 51 e UK Tel 0800 616 928 Fax 0800 616 927 e USA Tel 1 800 526
47. a Base setting affects only the Stable baseline condition For this condition to be activated the sig nal may not vary by more than the Delta Base value up or down over the time interval specified in the Stable baseline condition in the Watch instruction The Delta Peak setting affects only the Less than or valley and Peak max conditions when using the Watch instruction see UNI CORN user manual As a general guideline set the value to 2 3 times the noise level and 5 10 of the smallest expected peak height Delta_Base setting affects only the Stable_baseline condition For this condition to be activated the sig nal may not vary by more than the Delta_Base value up or down over the time interval specified in the Stable_baseline condition in the Watch instruction Delta_Peak 0 5000 mV Delta_Base 0 5000 mV Delta_Peak 0 14 pH Delta_Base 0 14 pH e p103 Strategy for AKTAexplorer 100 an 18 1150 11 e p104 WatchPar Pressure The Delta Peak setting affects only the Less than or valley and Peak max conditions when using the watch instruction see UNI CORN user manual As a general guideline set the value to 2 3 times the noise level and 5 10 of the smallest expected peak heigth Delta_Base setting affects only the Stable_baseline condition For this condition to be activated the sig nal may not vary by more than the Delta_Base value up or down over the time interval specified in the
48. aring you have some method s that you have not connected to a system most likely from imported methods using the Copy from external function in the Main Menu Connect the method s to the appropriate system and the dialogue disappears e p31 HD Troubleshooting Note an 18 1150 11 e p82 6 3 5 The Method Editor window does not fit on the screen If the Method Editor window does not fit on the screen and has scroll bars you may have the incorrect font size installed For pre installed Windows NT 4 0 the display screen resolution is set at 1024x768x65536 with Large fonts You need to install the Small fonts which requires that you have available the Windows NT Workstation 4 0 CD ROM or Windows 2000 shipped with your Compaq computer Insert the CD ROM and follow the directions on the screen Be sure to always install the latest NT4 service pack after installing something from the Windows NT 4 0 CD ROM 6 3 6 There are red instructions in a method Red instructions i e instructions with a red dot in a method are syntax errors and may be due to the following e The method was connected to the wrong system i e the strategy of the system is incompatible with the method e The method instructions do not correspond to the components you have chosen for your system Check your system components under Administration System Setup in Main Menu e The Copy function was used instead of Copy from external w
49. ate New User dialogue Create New User x Create a new UNICORN user The created user has default set as logon password User name David Full name p avid Wiggans Position Power user Home folder Drive ci bd Name DW kd OK Cancel Help Fig 4 8 Create New User dialogue 2 Enter the User name Full name of the user and Position as appropriate You can continue to define new users as long as the User setup dialogue is open Click on OK to close the dialogue If you close the dialogue by clicking on Cancel all changes made since you opened the dialogue will be lost 3 Itis possible to select an existing home folder by selecting a drive and name in the drop down menus 4 Click on New to create a new home folder for the user Select a drive from and enter a folder name in the Create New Folder dialogue If you choose to create a home folder on a network drive make sure that the drive is always accessible Click on OK to create the user to return to the Create New User dialogue e p59 4 Administration Create New Folder xj Drive c fui Folder name e di OK Cancel Fig 4 9 The Create New Folder dialogue Caution In a network installation always create home folders on a network Note drive which is accessible from all computers If you create a home folder on the C local drive it will only be accessible from the computer on which it was created A home fol
50. be unlocked using the locking password see Leaving and locking a system on page 42 We recommend that this authorisation is restricted to a few users in an installation The user who locks a system does not require this authorisation to unlock the same system Run methods Required for starting methods Manual interaction Required for issuing manual commands in System Control Pause Required for pausing a running process with the PAUSE button in System Control The PAUSE instruction in methods does not require explicit authorisation e p55 gt Administration an 18 1150 11 e p56 e Calibrate Required for using the Calibrate commands in System Control e Tune Required for using the Tune commands in System Control see Feedback tuning on page 89 e System settings Required for changing system settings with the Settings command in System Control see System settings on page 75 Any user may view the system settings but this authorisation is required to make changes to the settings e Edit MethodQueue Required for using the MethodQueue editor e Run MethodQueue Required for running MethodQueues e Edit global list s Required for saving a method as a method template an evaluation procedure a report format a column in the Column list or BufferPrep recipe also Quantitation tables and Mol Size tables if the Analysis module is installed as globally available It is also required for deleting me
51. blish a connection is logged on to the network e that the limit of 8 connections to the system has not been exceeded If you can establish a connection but cannot control the system the manual menu commands in System Control are grey check that no other user has a control mode connection and that you have sufficient access rights to control the system manually 6 2 3 Run data Connection in System Control displays a No x In System Control if the Run data option Connection says No 1 or No 2 e Check that the UNICORN PC Control board is configured according to the settings made during the installation of the program i e the same Control unit number Address and IRQ must be set at the Control board see Hardware installation on page 19 e The communication may also fail if the UNICORN PC Control board configurations conflict with other boards in the PC If this is the case select a free Address and a free IRQ during UNICORN installation and at the Control Board see Hardware installation on page 19 If the Run data option Connection says No 3 there is no contact with the communications interface e In Main Menu select Administration System Setup In the dialogue select the system with problems and click on Edit Check that the Troubleshooting Gi strategy pipe server name and the control number are correct according to installation at the local station physically connected to the system
52. chPar_Cond the Less than or valley and Delta Base WatchPar Conc Peak max conditions when using WatchPar pH the Watch instruction see UNI WatchPar Pressure CORN User Manual As a general WatchPar Sample guideline set the value to 2 3 times pressure the noise level and 5 10 of the WatchPar_Sample smallest expected peak height Flow Delta_Base setting affects only the Stable_baseline condition For this condition to be activated the signal may not vary by more than the Delta_Base value above or below the time interval specified in the Stable_baseline condition in the watch instruction AirSensor1 Sets the sensitivity of the air sensor Mode AirSensor2 flow cell The status No air Air of High Sensitivity AirSensor3 the cell is shown in Run data and Medium Sensitivi ty Low Sensitiv ity Flow from SystemPump SamplePump e p117 Strategy for AKTAexplorer 100 C 3 4 Curves Instruction Description Parameters UVI Set the specific signal curve on off for Store UV2 storing in the Result file The time ON OFF UV3 between samples determines the fre Time between Cond quency with which curve data is samples Cond recorded Conc pH Pressure Flow Temp Sample Pres Sample Flow AuxIn1 AuxOut1 AD900 A900Temp an 18 1150 11 e p118 INDEX A access 62 authorisation level items 54 defining levels 54 examples of groups 56 examples of levels 58 security 1 administ
53. cipe and BufferPrep pH The system will be washed at 25 ml min default vol ume 75 ml s System Wash 0 OFF 10 999 ml MethodBase Sets the base for method volume cal culation to either SystemPump or SamplePump Base SystemPump SamplePump AuxOut1 Sets signal on digital out 1 or 0 or 1 Make sure correct default value for digital out signal 0 or 1 is set in system settings Check auxiliary equipment for correct default value Signal 0 1 e p95 Strategy for AKTAexplorer 100 R AuxOut2 Sets signal on digital out 2 to 0 or 1 Signal Make sure correct default value for 0 1 digital out signal 0 or 1 is setin system settings Check auxiliary equipment for correct default value AuxOut3 Sets signal on digital out 3 to 0 or 1 Signal Make sure correct default value for 0 1 digital out signal 0 1 is set in sys tem settings Check auxiliary equip ment for correct default value AuxOut4 Sets signal on digital out 4 to 0 or 1 Signal Make sure correct default value for 0 1 digital out signal 0 or 1 is setin system settings Check auxiliary equipment for correct default value C 1 2 Flowpath Instruction Description Parameters InjectionPartial Selects the injection mode Injection VialNo Partial for the AutoSampler The 1 160 syringe dispenser fills the sample line Inj Volume with sample The valve turns and the 1 500 sample loop is filled b
54. click on the OK button Select OK to accept Share in the Type of Access dialogue Installing TCP IP on a non networked computer This is performed by the network administrator 1 an 18 1150 11 e p10 Open the control panel with Start Settings Control Panel 10 11 12 13 14 15 16 17 18 19 Network setup Gi Double click on the Network icon A question will be displayed asking if you want to install Windows NT 2000 Networking Click on Yes In the next dialogue select Wired to the network and click on Next Specify the network adapter to use by clicking on Select from list If your adapter is shown in the displayed list select it and click on OK Alternatively click on Have disk and insert the disk with the latest version of your driver Enter the path to it in the dialogue and click on OK Click on Next Make sure your selected adapter is shown in the list and click on Next Select TCP IP as the protocol to use Click on Next Select all default the services and click on Next Configure the TCP IP protocol Information specific to the network will have to be entered This information should be supplied by the network administrator Click on Next when ready Click on Next again to install the selected components Insert the Windows NT 2000 CD ROM disk and enter the path to it in the dialogue Example D Click on Continue A dialogue is displayed displaying where the file
55. ction Text State Action mode Not connected none Click on the System Connection icon or choose System Connect to establish a connec tion Control mode Controlled Click on the Disconnect by lt user gt from System icon or choose System Discon nect to leave the system but retain the connec tion with the System Control module You may leave the system locked or unlocked e p41 DD Installation an 18 1150 11 e p42 View mode Controlled by lt other user gt View mode Locked by lt other user gt View mode System is available In all modes you can choose System Disconnect or click on the Disconnect from System icon to disconnect the system from the control window The indicated user has a control mode connec tion Clicking on the System Connection icon as no effect The indicated user has left the system in a locked state Click on the System Connection icon to establish a con trol mode connection you must supply the locking password which is case sensitive or your logon password if you have Unlock locked sys tems authorisation A user has left the sys tem in an unlocked state Click on the System Connection icon to establish a control mode connection 3 5 3 Leaving and locking a system A running or End state system with a control mode connection can be left and locked by clicking on the Leave control of system icon or by selecting System Leave Control When the system is left
56. der can always be created on a network drive even if UNICORN is not installed for network control The computer only needs to be connected and logged on to the local network In general each user should have a separate home folder To create new folders under a home folder use the click on File in the Main Menu select New and then Folder Enter the new folder name in the dialogue This operation does not require special authorisation Any folder may be used as a home folder As a recommendation in network installations place the home folder on a drive which is addressed by the same drive letter from all computers in the network 4 2 4 Assigning Users Properties There are four groups of user properties that are accessible from the User setup dialogue User Advanced Access Folder and Systems and Instructions User Properties The first User Properties dialogue is for assigning user names passwords user groups and home folders The minimum number of characters in the password is defined when UNICORN is installed see Installation on page 19 The program may also be installed without password protection The case of letters in passwords is significant Caution Leaving the password as default can constitute a serious security risk an 18 1150 11 e p60 1 Select a user in the User setup dialogue and click on Edit to access to Users Properties dialogue Administration ci Caution For security reasons make sure that
57. e improved by cleaning the electrode then you must replace the electrode For further information read the Monitor pH C 900 User Manual If calibrating with the electrode outside the flow cell Remember not to remove the electrode from Solution 1 until the Read Value 2 button has become available the text on the button turns from grey to black e p47 3 Installation an 18 1150 11 e p48 Administration ci 4 Administration There are three main aspects of administration of a UNICORN installation e System administration e Access groups and user administration e Network administration where appropriate System administration concerns maintenance of software aspects of the installation including definition of connected systems and monitoring of system usage audit trails These activities are described in the present chapter System administration duties may also include routine monitor calibration see Calibrating monitors on page 44 Access groups and user administration concerns authorisation of access to the system and should at least in larger installations be the responsibility of one person or a small group These activities are described in the present chapter In a network installation maintenance of the network functions will normally be carried out by the computer staff responsible for the company s network Aspects of network administration relevant to UNICORN are considered in Chapter 2
58. ect System Tune in System Control Select Flow for Feedback Control Open the valves and start the pump with the column in line 2 Set P 0 05 I 9999 and D 0 Set the flow rate in Setpoint Press New parameters and the new values will apply 3 Note the response Increase the value of P until the actual flow rate oscillates with a constant period and amplitude When changing to new PID values set the new values and flow rate and press New parameters The flow rate must be changed in Setpoint every time the PID values are changed When the oscillation is satisfactory note the P value P0 and the oscillation period in seconds TO 4 Calculate suggested PID values for the required regulator type from the table below Feedback tuning GE Regulator P I D type P 0 5 Po PI 0 45 Po 0 83 To PID 0 6 Po 05 Ty 0 125 Ty 5 Adjust the PID parameters from these suggested starting values until the feedback behaviour is satisfactory 6 When satisfied press Save to save the PID parameter settings During tuning adjustments UNICORN displays the effects of the current parameters graphically The Output signal is the signal to the pump The Input signal is the actual flow rate B 2 Gradient tuning Tune gradient settings in the same way as flow rate settings with the exception that the Setpoint parameter is B The Input signal is the actual eluent concentration B as determined by the cond
59. ect the system type in the drop down list box If you want to connect a strategy to the system select a strategy in the drop down list box where all installed strategies are visible When all selections have been made click on the OK button to save the system in the system table If you click the Cancel button the system will not be written to the system table and you have to enter it yourself from within the UNICORN software in the Main Menu Administration System setup e p37 ED Installation an 18 1150 11 e p38 System Table Settings xi The installation program is about to create the system table and needs some additional information Enter a name that you want to use for the system defined below If you want to connect a strategy to the system select a strategy in the drop down list box Only strategies that have been installed are visible System name System System type Chromatography Strategy uomo N System settings Control board CU 900 Control unit 1 IRQ 10 Address 100 Fig 3 17 System Table Settings dialogue 17 After the installation is complete the computer must be restarted for the installed software to be properly configured Click on Finish to exit the Setup Complete dialogue and restart the computer Setup Complete Setup has finished copying files to your computer Before you can use the program the computer has to be restarted Remove any discs fro
60. ect to the set delay 0 100000 volume FractionCollector is stopped Start_at with the instruction Fractionation 0 4 Stop Base 1 2 Fractionation Stops fraction collection after the set Stop delay volume has been collected FeedTube During Fractionation or Peak_Fractionation FeedTube moves the tube rack forward one tube after the set delay volume has been col lected A fraction mark is given PeakFractiona Peak Fractionation enables only Tube type tion peaks to be collected The UV moni 1 2 tor is used to determine when there is PeakSize a peak to collect PeakSize determines 0 100000 max volume to be collected in each Start at tube To collect the peak in one tube 0 4 set PeakSizespeak volume If Base Peak FracParameters are not set in 1 2 the method default values in system settings will be used Tube change is delayed with respect to the delay vol ume To stop PeakFractionation use the instruction Peak FracStop Peak Frac Sou Sets the source UV or AD900 for Mode rce peak detection UV AD900 Peak FracStop Stops peak collection after the set delay volume has been collected e p105 Strategy for AKTAexplorer 100 an 18 1150 11 e p106 Peak_FracPara metersUV UVI1 Parameter settings for Peak_Fractionation Choose between Slope and Level to detect peaks To obtain suitable value for Slope per form a test run and measure the slope at the beginning and the end of the s
61. es a user defined message Messages may be issued in Screen or Noscreen mode Message Mode New_ Chromato gram Pause Opens a nw chromatogram in the result file All subsequent data until the next New_Chromatogram instruction or the end of the method will be stored in the new chromato gram Places the system in the Pause state for the specified length of time Name Time Ready Signals that a next step in a method MethodQueue may start provided that the next step runs on a different wet unit Set_mark Sets a mark in the chromatogram Selecting the Set_Mark will display the message text Mark text Inserts a comment in the method Comments do not affect method exe cution Comment e p109 Strategy for AKTAexplorer 100 C 2 Manual control an 18 1150 11 e p110 C 2 1 Pump This group contains the same instructions as the Pump group in method instructions with the exception of no MethodBase instruction C 2 2 Flowpath This group contains the same instructions as the Flowpath group in method instructions with the exception of no QuantitationData instruction with the following addition SyringeEnd are used when the syringe is replaced SyringeEnd wash solvent is aspirated from the wash solvent SyringeHome the syringe contents will be dispensed to the syringe waste NeedleFront and NeedleHome is used when the sample needle is replaced
62. etup program is launched and you will see the Welcome dialogue Click on Next to continue Welcome x Welcome to the Unicom Setup program This program will install Unicom on your computer Itis strongly recommended that you exit all Windows programs before running this Setup program Click Cancel to quit Setup and then close any programs you have running Click Next to continue with the Setup program WARNING This program is protected by copyright law and international treaties Unauthorized reproduction or distribution of this program or any portion of it may result in severe civil and criminal penalties and will be prosecuted to the maximum extent possible under law Fig 3 3 Welcome dialogue Installation 6 The license agreement must be accepted to install UNICORN Click on Yes to continue the setup program UNICORN Software License Agreement x Please read the following License Agreement Press the PAGE DOWN key to see the rest of the agreement Licence Agreement and Warranty Statement Important Please read the Licence agreement below carefully The right to use this Amersham Pharmacia Biotech AB Software Product is sold only on the condition that the customer agrees to the following Licence If you do not agree to the terms of the Licence you may return the software for full refund terminating the licence as set forth below Licence Agreement In retum for the payment of the one
63. f migration from a previous version of UNICORN is required please contact your Amersham Biosciences Service Representative for instructions 3 2 System requirements For the hardware software and network requirements please refer to Appendix A 3 3 Hardware installation In most cases an installation of your system will be performed by Amersham Biosciences authorised personnel If your system is not pre installed then follow the steps below to install the expansion card in your PC This procedure is only required in stand alone installations i e systems not connected to a network and on local computers in network installations 1 Turn off the power to your PC and remove the power cable from the mains socket 2 Open the PC cover Refer to your PC documentation if you are not sure how to do this e p19 ww Installation an 18 1150 11 e p20 3 Locate an empty ISA slot for CU 900 and UNICORN Control Board AT expansion cards Locate an empty PCI slot for CU 900 PCI cards Take the expansion card out of the anti static bag Handle the card by its edges and avoid touching the electronic components as far as possible because discharges of static electricity can permanently damage electronic components on the card If you are working in a room where static electricity tends to build up discharge any electricity from your body by touching an earthed metal surface e g a water tap or radiator before handling the ca
64. formation printing 65 viewing and changing user definitions 64 user groups and users creating on local computer 14 creating on NT domain with TCP IP network 9 creating on NT workstations connected to a Novell server 14 W watch instructions 107 Windows NT 85 Wizard report generator 69 Z Ziegler Nichols method 90 Amersham Biosciences e 127
65. global procedures report formats BufferPrep recipes columns or a users file already exist you will be asked if you want to replace the corresponding files Normally you should answer No When any of the global user files are replaced a backup file is created from the old file The next time UNICORN is started these backup files if they exist are checked against the new files If any differences exists between the files the user is presented with a dialogue where data that exists in the backup file but not in the new file can be restored 9 Select the disk drive where the program is to be installed and click on the Next button to continue This should be a physical disk drive usually c on the computer where you are installing UNICORN not a network disk drive e p29 ED Installation Select Drive The UNICORN software have to be installed to a folder nomed Unicorn located in the root directory of the selected rive Select the drive that you want to install the UNICORN software to and press the NEXT button to continue Available disk drives on the system Destination Path C UNICORN lt Back Cancel Fig 3 7 Select Drive dialogue 10 Choose whether the setup type is stand alone or network For a stand alone installation the network options settings are ignored A stand alone installation can be either a local station or a demo station If you want to install a demo station make sure that Demo system is
66. have access from the Systems list and the access level of the user from the Access level drop down list User Properties Instructions It is possible to define the user level at which manual instructions should be available to the user and system sounds Select a user name in the User setup dialogue click on Edit to access to Users Properties dialogue Click on Instructions in the menu tree and define the user levels in the by clicking on the check boxes 4 2 5 Changing user passwords Every user can change his or her own password with the Administration Change Password command in the Main Menu Enter the old password and the new password in the appropriate fields Passwords are not displayed explicitly in this dialogue The password will not be changed if either the old password is incorrect or the two copies of the new password differ from each other In addition a user with access to user group User setup Levels can change the password for any user To change a password for another user open Administration User setup select the user and enter a new password in the Password field see Changing user passwords on page 63 Change passwords regularly and avoid obvious passwords like secret and open_sesame for maximum security If you forget the password for the only user with User setup Levels access you must re install the default user as described in We recommend that only one user in an installation or netwo
67. hen importing a method from a diskette e The wrong system may have been selected in the Save As dialogue in Method Editor e You may also have templates not intended for your system which might be the case for custom designed systems e The systems strategy has been updated with a new strategy that differs in the instruction set There are several actions that you can take e Check that the method has been connected to the correct system either in the System Method Connection dialogue when using the Copy from external dialogue or in the Save As dialogue in Method Editor e For custom designed systems go to the Method Editor select the red instruction and either delete it or replace it with a corresponding instruction if available from the Instruction box Repeat this for all red instructions before saving the method Troubleshooting Gi 6 3 7 I ve logged out of Windows NT 2000 and then logged in again but can not get system connection in UNICORN only for local systems not remote If you shut down Windows NT 2000 using the command Start Shutdown Close all programs and log in as a different user you will not be able to obtain a System Control connection in UNICORN the next time you or another user logs on This is because the aforementioned shutdown procedure automatically shuts down a number of processes including those needed for system connection that are only started when the computer is booted up In other words y
68. hould only be used at flow rates generating drops Tube No synchronisation of collection Note The delay volume must be changed when the parameter TubeChange is altered to or from Accumulator see the manual for the correct delay volume Peak_Frac_Sou Sets the source UV or AD900 for Mode rce peak detection UV AD900 Peak_FracPara UV1 Parameter settings for Mode metersUV Peak Fractionation Choose between Level Slope Slope and Level to detect peaks To MinWidth obtain suitable value for Slope per form a test run and measure the slope at the beginning and the end of the smallest and flattest peak you want to collect using Operations Differenti ate in Evaluation See UNICORN manual for more details For level fractionation the Peak Fractionation is started when the UV signal rises above the set level value and is ended when the UV signal goes below the set value Time min must be used as base MinWidth sets the minimum Peac Fractionation time Peak Fractionation continues at least until MinWidth is reached even if the signal is fluctuating 0 10 1500 min Level 6000 to 6000 mAU StartSlope 0 10 10000 mAU min EndSlope 0 10 10000 mAU min e pl13 Strategy for AKTAexplorer an 18 1150 11 ep114 100 Peak_FracPara AD900 Parameter settings for Level metersAD900 Peak FractionationAD900 5000 TO 5000 The Peak_Fractionation is started mV when the UV
69. hout special operating system utilities All methods result files and folders within a selected folder will be deleted when the selected folder is deleted Administration ci User properties m x C Dave David Wilson Folders User Advanced Elon Access Folders FR Systems 44 Instructions paeba Q Are you sure you want to delete 3 folder s a c bengt c Colin c Frank Yes No New Delete OK Cancel Help Fig 4 13 Deleting a folder from User Setup You cannot delete e a home folder to which a user is assigned To delete such a folder you must first either delete the user or change the home folder assignment for this user Select the user in the User Setup dialogue and assign a different home folder e a folder to which several users share access To delete such a folder remove the access rights from each user first 4 2 10 Printing user setup information You can print the settings for selected users 1 In the User Setup dialogue click on the Print button The Print dialogue is displayed 2 Select individual users for which you want to print information or click on the Select All button 3 Check the boxes for the Print Items that you want to include 4 Click on OK to print e p65 4 Administration an 18 1150 11 e p66 4 3 Audit trails The audit trail accessed under Administration Audit Trail in the Main Menu provides a full record of UNI
70. ill ask for the template disk when it is needed When installing strategies you will be prompted for a name for the strategy that is being installed You can either keep the default name or enter another name Enter Information i x Enter a name for the strategy maximum 8 characters lt Back Cancel Fig 3 15 Enter Information dialogue an 18 1150 11 e p36 16 Installation If you click on the Select button the template files and wizard will be installed to the folder where the selected strategy is located The Cancel button aborts the template installation In both cases you are returned to the Strategy amp Template Installation dialogue Select Strategy x To install the template files select the correct strategy and click on the SELECT button To abort the template installation click on the CANCEL button Strategies installed on the system Fig 3 16 Select Strategy dialogue Choose to install additional strategies if you have more than one system configuration Strategies are installed independently of systems A strategy is assigned to a system when the system is defined The System Table Settings dialogue is only displayed when UNICORN is installed for the first time and only if a system table is not already present The dialogue will be shown for each system defined in the System Setup dialogue see step 14 above Enter a name that you want to use for the system and sel
71. in cases the D parameter can reduce the oscillations introduced by a PI regulator D can have values between 0 and infinity where larger values have greater effect and a value of 0 has no effect Most often a simple PI regulator is preferable for control of flow rate and BioProcess System is configured by default with the D parameter set to zero setpoint stationary response P regulator PI regulator Fig 2 1 A simple P regulator left gives a stationary error A PI regulator right eliminates the stationary error but introduces stable oscillations in the response e p89 B Feedback tuning Note an 18 1150 11 e p90 Tuning a feedback control system in practice is largely a matter of trial and error The following recommendations summarize the Ziegler Nichols method for finding suitable PID values small empirical adjustments in the suggested values may be required for optimal feedback control In situations where more than one instrument has the possibility for feedback control in the Tune window a range problem may occur if the actual range is different between the two instrument loops Specifically a Setpoint out of range warning may occur if the lower range has been used before the higher range Action If more than one feedback control possibility exists choose the same parameter twice in the Feedback Control window B 1 Flow rate tuning 1 Make sure that the flow path is open Sel
72. is issued or the result file is closed Data are also saved to disk at pre set intervals during a run minimising data loss in the event of power or communication failure The capacity of the data buffers is 16000 points for up to sixteen monitors as listed in the Curves group of System Settings in System Control If a buffer is filled during a run the number of points is halved by deleting every second point and the sampling frequency for subsequent points is halved The initial sampling frequency for each monitor is set in the system strategy and can be viewed and changed in the Curves group of System Settings For AKTAdesign systems the sampling frequency for the UV curves is set to 10 samples s 10 Hz For BioPilot System strategies the sampling frequency is normally set to 0 5 or 1 sample s 0 5 or 1 Hz depending on the type of monitor Atan initial sampling frequency of 10 samples per second 10 Hz the following resolutions apply for the curves Technical specifications co Duration Sampling frequency No of points Resolution s point 0 27 min 10 Hz 0 16000 0 1 27 53 min 5 Hz 8000 16000 0 2 53 107 min 2 5 Hz 8000 16000 0 4 107 203 min 1 25 Hz 8000 16000 0 8 To ensure maximum resolution for part of a run issue a New Chromatogram instruction at the beginning of the part This empties the data buffers and resets the sampling frequency to that specified in the system settings e p37
73. ith a request to give a valid user name and password The user name will be the ID previously entered in the Connect As field Give the password and click on OK Install UNICORN see Installation on page 19 select Network version Re boot the PC and log on as one of the users that will run UNICORN The Select NetWare Logon dialogue will be displayed The network administrator must supply the Default Tree and Context to use Select the Default Tree and Context radio button enter the information from the network administrator and click on OK Connect the shared folder again this is necessary since each user has his own connected network drives Repeat steps 1 2 3 5 and 6 above and make sure the Reconnect at Logon option is checked Start UNICORN and set up system definitions and user profiles see Installation on page 19 e pl7 2 Network setup an 18 1150 11 e p18 Installation EB 3 Installation This chapter describes how to install UNICORN software 3 1 Installation summary The following installation procedures are required before UNICORN systems can be used 1 Back up files if migrating from UNICORN 3 10 to UNICORN 4 0 2 Set up the network environment for network installations only 3 Install UNICORN hardware and software 4 Define access levels for the installation 5 Define users with home folders and access profiles 6 Check the system settings for the attached systems Note I
74. les Current Settings Column Files v 2 f Cancel Fig 3 11 Start Copying Files dialogue 14 If you have chosen to install systems the system installation dialogue is displayed after the file copy process Select a system and click on the Change button to set up the system When all systems that are connected to the station have been defined click on the Next button to continue e p33 ED Installation System Installation x Instrument settings for the installation Select a system and press the CHANGE button to edit the settings for the selected system When all systems physically connected to the computer have been defined press the NEXT button to continue r Select System System C System2 C System3 C System 4 r Settings Control Unit Control Board IRQ Address lt Back Next gt Cancel Fig 3 12 System Installation dialogue When you click on the Change button the System Setup dialogue is displayed System Setup Instrument setup for the system installation Select the control board and set the resource settings for the system Press the OK button to save the changes and return to the System Installation Dialogue The CANCEL button ignores the changes The DEFAULT button gives the default resource settings for the selected system type and the CLEAR button clears all fields If you need additional help press the HELP button Sys
75. list Check the boxes to set the Password age Password uniqueness Account lockout Automatic workstation lock An additional password or Electronic signature is also defined in this dialogue User properties xj e Dave David Wilson Advanced H oe J m Password age on Access Iv Expires in po days E94 Instructions r Password uniqueness gt IV Remember E passwords m Account lockout W After E bad logons r Automatic workstation lock M Lock after F5 minutes r Signature Password Confirm Cancel Help Fig 4 11 The User properties Advanced dialogue User Properties Access Folders and Access Systems In the User setup dialogue and click on Edit to access to Users Properties dialogue Click on Access and Folders in the menu tree Select which folders the user shall have access to from the check box list Up to 20 folders can be set up to be shared Selecting a folder here will give the user access to all files and folders therein Folders that are not selected in this list will not be visible in the methods or results panel of the Main Menu All users should be given access to the Failed folder on each local station in a network installation This will ensure that users can access results saved in the Failed folder in the event of a network communication error Caution Administration ci Click on Systems to select the system s to which the user will
76. lue is valid for all runs and remains until you again change the value or return the setting to its default value Of course you can temporarily change the setting within a method as described in the previous point Changes to the default settings should be made when the system is installed Also certain settings may need to be adjusted if system components are changed e g alarm and warning limits or for specific run purposes e g monitor and curve settings For strategies where you can select the system components only the settings for the selected components will be displayed Assigning a new value to the actual system setting is performed in the System Control module and Audit trail system setup access is required to make such changes see User access groups on page 54 1 To access the settings click on the appropriate System Control icon on the taskbar and select System Settings The Instructions dialogue for the connected system is displayed Basic Explorer 10XT Alarms Instructions x m Instructions Alarm Pressure Parameters foi Mode Mains Made Enabled C Disabled High larm 25 000 MPa naed ooo C Specials Low larm 0 000 MPa 3 wash Alarm Pressure HighAlarm 2 Cc il Mode Enabled 1 Meno HighAlarm 15 000 MPa kali LowAlarm 0 000 MPa Alam UW1 LowAlam Mode Disabled 0 000 ja MPa HighAlarm 6000 000 mAU Curves andre RNANA NAON mali Set Selected Parameter To Strategy Default Value
77. m their drives and then click Finish to complete the UNICORN Software setup and restart the computer Fig 3 18 Setup Complete dialogue Note Installation EB 3 4 2 Installing selected software components after the initial installation If your UNICORN installation should be damaged for example due to accidental file deletion or hard disk failure or if you want to install additional systems strategies or templates you can use the installation program to re install selected parts of the software The installation program detects the presence of existing UNICORN files and suggests the components to install Check some or all of Program files User information Strategy amp template files System installation and Manuals according to which part s you wish to install The appropriate parts will be copied into the existing folder structure For network installations remember to log on to the network before installing any UNICORN software components Program files Before re installing the program files be sure that the UNICORN software is not running Check the Program files option in the Components list to re install UNICORN This will not affect any existing method or result files in the system The program is always installed locally even in network installations User information User information contains several sub components global procedures global report formats global BufferPrep recipes column files and the users
78. mallest and flattest peak you want to Mode Level Slope MinWidth 0 01 1500 min Level 6000 to 6000 mAU collect using Operations Differenti StartSlope ate in Evaluation See UNICORN 0 01 10000 manual for more details For level mAU min fractionation the Peak Fractionation EndSlope is started when the UV signal rises 0 01 10000 above the set level value and is ended mAU min when the UV signal goes below the set value Time min must be used as base MinWidth sets the minimum Peac_Fractionation time Peak_Fractionation continues at least until MinWidth is reached even if the signal is fluctuating Peak_FracPara AD900 Parameter settings for Level metersAD900 Peak FractionationAD900 5000 to 5000 The Peak Fractionation is started mV when the UV signal rises above the set MinWidth level value and is ended when the UV 0 10 15000 min signal goes below the set value MinWidth sets the minimum Peac_Fractionation time Peak_Fractionation continues at least until MinWidth is reached even if the signal is fluctuating Accumulator Use this instruction to wash the accu Strokes Wash mulator The flow rate must be gt 0 OFF 0 1 10 Reset_Frac_Pos ition This instruction will reset the fraction collector Thus fractionation set to start at next position will start in the first position The instruction will reset next position for all tube types Strategy for AKTAexplorer 100
79. meout is reached The method execution is then continued Instruc tions at the same breakpoint as Hold until but in the method placed after Hold until will be executed AFTER the method execution is con tinued Signal Test Value Timeout C 1 7 Other Instruction Description Parameters Base Sets the method base to time volume column volume or SameAsMain A column can be selected if defined in the column list enabling linear flow rates to be used Column Any can also be selected Column Base Volume Block Calls a block unconditionally Block Continue Resumes execution of a held method End Block Returns control from a block to the point at which the block was called End Method Terminates method execution an 18 1150 11 e p108 Evaluate Strategy for AKTAexplorer 100 Calls an evaluation procedure which must be stored together with the method Calling an evaluation proce dure closes the current chromato gram and any data colleted subsequently will be stored in a new chromatogram Procedure Hold Places the system in Hold state Loop Repeats the instructions between Loop and Loop_End a specified number of times Loop must be paired with Loop_End in the same method block No of loops Loop_End Ends a Loop instruction Loop_End must be paired with Loop in the same method or block Message Generat
80. ms later or change the settings for an already defined system run the setup program once again with only the System Installation option checked in the Component Selection dialogue 15 If you have chosen to install strategy and or template files the Strategy amp Template Installation dialogue is displayed If the correct strategy is visible in the list mark that strategy and click on the Install button If you have the strategy template files on a diskette or another source click the Have Disk button to locate the strategy template files e p35 ED Installation Strategy and Template Installation x Select the strategy you want to install and press the INSTALL button If your strategy template is located on a floppy or on another source press the HAVE DISK button to locate it When all strategies templates are installed press NEXT to continue Options IV Install strategy IV Install template Strategy template AKTA Basic 10 with Frac 900 AKTA Basic 10 with Frac 950 AKTA Basic 10 XT with Frac 900 AKTA Basic 100 with Frac 950 AKTA Explorer 10 5 with Frac 900 xl AVTA Polo INF aithle Pee NEN Have Disk Cancel Fig 3 14 Strategy amp Template Installation dialogue If the options for both strategy and template are checked the template files are installed automatically together with the strategy If the strategy and template files are located on different diskettes the setup program w
81. n address in the UNICORN software installation is the number listed in the Address DualPort column The manufacturing default setting for the address is no 12 and for this setting D0000 should be entered as the address in Jumper IRQ setting W2 3 W3 5 W4 10 WS 11 the UNICORN software installation No 52 1 S2 2 52 3 S2 4 Address DualPort 0 ON ON ON ON 40000 1 OFF ON ON ON A4000 2 ON OFF ON ON A8000 3 OFF OFF ON ON AC000 Installation EB ON ON OFF ON 0000 OFF ON OFF ON 4000 ON OFF OFF ON 8000 OFF OFF OFF ON BCO000 ON ON ON OFF C0000 of NT Nf ay F OFF ON ON OFF C4000 10 JON OFF ON OFF C8000 11 OFF OFF ON OFF CC000 12 ON ON OFF OFF D0000 13 OFF ON OFF OFF D4000 14 JON OFF OFF OFF D8000 15 OFF OFF OFF OFF DC000 6 Install the expansion card in an empty PCI slot and close the cover on your PC 7 Connect the card to the liquid handling module CU connector using the communication cable provided Additional liquid handling modules up to a total of four may be connected 3 4 Software installation UNICORN will normally be installed by your Amersham Biosciences representative Follow the instructions below if you need to install the program yourself If the system is connected to the network and installed to support remote control make sure that the same version
82. n any computer in the network not possible during scouting or MethodQueue runs by any user with sufficient access rights see above e A UNICORN user may connect to a system with up to eight different remote workstations in the network Each successive multiple instance of the user automatically establishes the same System Control connections as the first instance when the logon is performed Multiple instances are however treated by UNICORN as separate users although they are not distinguished in the System Control workspace display and only one of the instances may maintain a control mode connection to a system Multiple instances may also disconnect and connect their System Control modules independently of each other once the logon is performed See System connections on page 11 3 6 Calibrating monitors Certain system monitors notably pH monitors need to be calibrated regularly for correct results According to the routines established in the laboratory or process department monitors may be calibrated at pre set intervals by the system technician or calibrated as required by the user before each run This section describes the calibration procedure For AKTAdesign systems the pump pressure offset and pH must be calibrated regularly Installation EB Monitors can only be calibrated by users with Calibrate Tune access All monitors can be calibrated from UNICORN to convert monitor signals to appropriate units for display
83. ng curve 77 hysteresis 77 store 78 time between samples 78 e 125 um 18 1150 11 e 126 software requirements 85 specials system settings instructions 112 stand alone installation 86 static electricity precautions 20 STORE ON 81 store setting 78 strategy 53 finding description of instruction 93 for AKTAexplorer 100 93 selection 50 strategy definitions deleting 53 strategy file error 79 system audit trails 67 component selection 51 connection 40 connection modes 41 connections management 40 disconnect 42 disconnecting 43 leaving and locking 42 naming 50 requirements 85 system control monitor signals not appearing 81 system definitions defining new system 50 deleting 52 editing 52 management 49 system run hours 69 system settings accessing settings 75 instructions 111 introduction 75 system type 50 T TCP IP installing on computer 10 time and date correction 83 time between samples setting 78 U UNICORN access problems 79 cannot quit or logoff 81 in the network environment 5 installation 16 installation summary 19 installing in NT domain with TCP IP 13 installing on network with IPX SPX Novell server 13 installing software 25 operations after installation 49 problem re establishing system connection 83 system administrator 6 unlocked system 43 user administration 58 defining new user 58 deleting 64 home folder selection 61 identification 58 selecting user accessible folders 62 setup in
84. nstallation Fig 3 1 The PCI expansion card When you are installing the UNICORN software and the setup program asks for the card settings enter the correct values for IRQ and address If for some reason the default settings can not be used on a specific computer for example there is a conflict with another device that it already installed the settings have to be changed Fig 3 2 Dip switch settings for the CU 900 PCI card ep2l ww Installation an 18 1150 11 e p22 CU 900 AKTA systems ISA Alternative IRQs for this card are 3 4 5 7 10 11 12 and 15 The manufacturing default setting is IRQ10 The address is made up of two parts the base address and the node address Valid base addresses for this card are any hex address between 0 and FFO the node address can be altered between 1 4 The manufacturing default setting should be updated to reflect the current default for the address 100 base address 100 and node address 1 Note that this is computer dependent To change the settings for the address refer to the tables Node Offset S1 7 S1 8 address 1 0 ON ON 2 400h OFF ON 3 800h ON OFF 4 C00h OFF OFF Installation EB Base address 100 offset S1 1 51 2 ON 51 3 ON 110 offset OFF ON ON 120 offset 130 offset ON OFF OFF OFF ON ON 140 offset ON ON OFF 150 offset 160 offset OFF ON ON O
85. o the network The user interface for UNICORN does not however need to be started on the local station System Control from a remote station is managed through network level routines which are started at log on time on the local station A local station can be used to control the chromatography system directly connected to the PC without logging on to the network The station must however have been logged on to the network once previously so that the necessary files are copied from the network server to the local computer This copying is performed automatically Method and result files stored on the network drives will of course not be accessible For runs performed in this stand alone mode where the result file is directed to a network drive the results will be saved in the Failed folder on the local station It is still required that the user log on to the local Windows NT 2000 station using a valid user ID 2 2 Requirements The following are minimum network requirements for running UNICORN in a network installation e Windows NT version 4 0 workstation or Windows 2000 workstation ep 2 Network setup an 18 1150 11 e p8 e A valid network card see Appendix A e A valid network connection e The user must have the right Access this computer from network to connect to the local station in remote control e Protocols and services such that named pipes are usable over the network e Protocols and service
86. on 36 Instructions Alarms group 75 Leave Control 43 New system 51 Open Log File 68 Password and System Control Window Settings 32 pH calibration 46 Select Components 28 Select Drive 30 Select Strategy 37 Setup Complete 38 Setup Type 31 Start Copying Files 33 Strategy amp Template Installation 36 System connect 40 System Installation 34 System Setup 34 System setup 50 System Table Strategy 38 UNICORN Software License Agreement 27 User information 27 Welcome 26 E edit system definitions 52 error cannot connect to system 80 connections not available 80 couldn t create result file 81 Method System Connection dialogue keeps appearing 81 No x 80 strategy file error 79 evaluation problems 83 procedures 83 F failed folder access 2 feedback control aims 89 feedback tuning 89 flow rate tuning 90 flowpath instructions 96 manual instructions 110 folder user access 62 e121 um 18 1150 11 e 122 G generating report 73 from the main menu 69 global audit trails 66 gradient tuning 01 H hardware installation 19 requirements 85 home folder deleting 64 selection 61 hysteresis setting 77 installation address setting 22 hardware 19 operations required after installation 49 selecting the components 28 selecting the type of system 35 setting IRQ and address 20 stand alone 86 UNICORN software 25 installing program files after the initial installation 39 software components after the initial
87. on Set The new setting will take effect from the time the change is made You can also start a new audit trail file at any time by clicking on Now This will not affect the automatic setting e g if the audit trail is set to renew every Monday and you click on Now on a Friday a new file will be started immediately and another new file will be started on the following Monday Administration ci 4 3 3 Backing up audit trail files Click Backup in the audit trail window to make copies of audit trail files Choose whether to copy or move the files and click on Backup The Move alternative is recommended to save disk space Backup operations are recorded in the audit trail System run hours In the system audit trail click on Run hours to display the accumulated run time for the system i e the time the system has been in manual or run mode Run hour values show the number of hours that the system has been used for manual or method controlled runs The Run hours record is useful in following up expected and actual lifetimes for liquid handling components Click on Reset to reset the accumulated run hours to zero The reset time is recorded in the audit trail 0 0 Hours Fig 4 17 Run Hours in the system audit trail 4 4 Report Generator Wizard UNICORN 4 0 contains a report generator Wizard for registration of errors or problems that you have detected or that occur during a run The Report Generator Wizard takes you through the
88. operators Process operators are allowed to run and pause methods and MethodQueues but may not perform any other operations 4 2 3 Creating new users All UNICORN users are identified by a username and password A new installation is provided with a default user username default password default This user provides unrestricted access to all UNICORN functions As part of the installation procedure new users should be created with passwords and restricted access rights as required The default user should be deleted or redefined to prevent unauthorised access to the system Maintenance of user authorisation information is the responsibility of the system administrator In a newly installed system log on as user default To define edit or delete users choose Administration User setup in the Main Menu To use this menu command you must have authorisation see above All user administration is performed from this dialogue User Setup x fo Define users and their security attributes m Access groups Each user is assigned to an access group This group defines access to different functions in UNICORN When changing a group all users Access groups assigned to that group are affected m Users Name Full name Group default default Administrator Fig 4 7 The User Setup dialogue Administration ci 1 Tocreate a new user click on New in the User Setup dialogue and enter the username in the Cre
89. ou must restart the computer in order to obtain a system connection in UNICORN 6 3 8 Print screen does not send a copy of the screen to the printer Print screen only makes a copy of the screen to the clipboard and not to the default printer If you wish to make a print out of the view on the screen press the lt Print Scrn gt key and paste the image from the clipboard into an appropriate program such as Microsoft Paint and then print out the image 6 4 Evaluation problems 6 4 1 Incorrect date and time The date and time recorded in the result file are taken from the PC system clock setting If these are not correct check the system clock setting 6 4 2 Evaluation procedure aborts Instructions in an evaluation procedure address curves by identification number irrespective of curve names Make sure that the curves processed when the procedure is executed are compatible with those processed when it was recorded An evaluation procedure aborts if you try to store resulting curves at the position of an original raw data curve e p83 HD Troubleshooting an 18 1150 11 p84 6 5 KTAdesign system specific problems 6 5 1 Connected to a system but no system contact In System Control if the Run data option Connection says Yes but the option Instruments says Scanning and there is no contact with the system after a period of waiting check if e The chromatography system is turned on e All cable connection
90. out the problem Reproducibility ep 1 4 Administration an 18 1150 11 e p72 Attachments You have the option of attaching result files method files and or log files to the report 1 To make an attachment first select the appropriate tab Result method System log or Global log r Files Result Method System log Global log Baseline example 1 res Delete r System information IV Computer amp operating system information TO ARTE hardware information IV Integrity check Emit Preview lt Back Next gt Cancel Help 2 Click on the Add button and browse in the displayed dialogue to find the file that you want to attach 3 Select a file and click on Open The selected file is added to the tab in the Attachments dialogue System information can also be included in the report by checking the appropriate option boxes By default the following options are checked Computer amp operating system information This summarises information about the computer and operating system e g type of processor processor speed RAM memory hard disk capacity and printer AKTA hardware information This summarises information about the specific AKTAdesign hardware e g the instrument and PROM version for every instrument that is connected Administration ci Integrity check When UNICORN is installed a checksum calculation is performed on the s
91. p Gi ORIO Chromatography LJ system aa PN ARDA PC with UNICORN Network sever Chromatography system PC with UNICORN Chromatography system 0508 PC with UNICORN PC where UNICORN PC with UNICORN is NOT installed Chromatography system Fig 2 1 A network installation with 4 chromatography systems and 5 workstations PCs The chromatography systems physically connected to PCs 1 and 5 can be controlled locally Alternatively any of the PCs with UNICORN installed can be used to remotely control any of the chromatography systems via the network In this example PC 4 is connected to the network but it cannot be used to control any chromatography systems since it does not have UNICORN installed Note also that the server does not have UNICORN installed and is not involved in the control process per se Creating user groups and users of UNICORN This is performed by the network administrator 1 Logon to the Windows NT 2000 domain controller with administrative rights 2 Enter the User Manager for the domain by selecting Start Programs Administrative Tools Common 3 Create a group that all UNICORN users will be part of Select the User menu and the New Local or Global Group item 4 In the dialogue that is displayed enter a suitable name for the group and click on OK e p9 2 Network setup Create the users that will run UNICORN Select the User menu
92. pensation consists of a compensation factor together with a reference temperature Con dRefTemp All conductivity values will then automatically be converted to the set reference temperature The factor is expressed in percent age increase of conductivity per C increase in temperature If the CompFactor is unknown a general approximate value of 2 can be set for many common salt buffers 0 off Tc is shown on monitor dis play if CompFactor gt 0 CompFactor 0 9 9 CondRefTemp Selects the reference temperature to which the measured conductivity values will be converted Con dRefTemp is active when a factor of CondTempComp is selected RefTemp 0 99 9 C pHTempComp Sets the temperature compensation on or off For more accurate meas urements during temperature changes the pH measurement can be temperature compensated When using pHTempComp it is important that the temperature of the pH elec trode is the same as that of the con ductivity flow cell since that is where the temperature is measured pHTempComp ON OFF Strategy for AKTAexplorer 100 Flow scheme in System control Connect the flow cell to the air sen sor control box according to the instruction manual Then set corre sponding air sensor number to the sensitivity needed Parameter Flow from selects the flow source for the air sensor flow cell WatchPar_UV1 The Delta Peak setting affects only Delta Peak Wat
93. r Installing IPX SPX on the computer 1 2 9 Open the control panel with Start Settings Control Panel Double click on the Network icon A question will be displayed asking if you want to install Windows NT Networking Click on Yes In the next dialogue select Wired to the network and click on Next Specify the network adapter to use by clicking on Select from list If your adapter is shown in the displayed list select it and click on OK Alternatively click on Have disk and insert the disk with the latest version of your driver Enter the path to it in the dialogue and click on OK Click on Next Make sure your selected adapter is shown in the list and click on Next Select NWLink IPX SPX Compatible Transport as the protocol to use Deselect TCP IP Click on Next 10 Select all default the services and click on Next 12 Select Client Service for NetWare and click on OK epld 2 Network setup an 18 1150 11 e p16 13 14 15 16 17 18 19 20 21 23 23 24 25 Click on Next Click on Next again to install the selected components Insert the Windows NT 2000 CD ROM disk and enter the path to it in the dialogue Example D Click on Continue A dialogue is displayed displaying where the files will be read Click on Continue The bindings are displayed Click on Next to continue Click on Next to start the network Select the Workgroup radio button
94. r interval may slow down the user interface response The control functions in UNICORN performance will however not be impaired If you add system definitions remember to grant access to the new systems to the appropriate users see System requirements on page 19 System name Purifier 100 System type Chromatography pd Strategy Information Component Pipe server name pav Control unit number 1 4 fi x Auto save interval E minutes Cancel Help Fig 4 2 New System dialogue Selecting system components Some system strategies allow you to define the specific components included in the system 1 In the New System dialogue click on the Component button The Component dialogue is displayed 2 To include a component in the system select it in the Component list field and check the boxes for the components you wish to add e pdi 4 Administration ss 3 When all selections have been made click on OK Component x Select components AutoSampler ColumnYalve Y2 ColumnYalve Y3 OutletValve Y4 SampleYalve Y5 BufferYalveA1 YG FlowDirectionYalye Y7 Valve V8 Valve V9 Cond monitor pH Cell K K K K K KK K K K Sample pump Airsensor 1 Airsensor 2 Airsensor 3 Fig 4 3 Component dialogue Note 1 The strategy contains instructions for all possible components However instructions are shown only for the components selected for the current s
95. ration overview 49 user 58 AKTAdesign system problems 84 alarm activation 77 alarms display 76 introduction 76 system settings instructions 111 alarms and monitors instructions 99 manual instructions 110 audit trail system setup 55 audit trails 66 backing up audit trail files 69 examining 66 global 66 printing 68 renewing audit trail files 68 system 67 viewing older audit trails 68 authorisation items 54 authorisation profile example development staff 57 process operators 58 process supervisors 57 system administrator 56 57 autosave interval value 51 BioPilot System sampling frequency 86 buffer capacity 86 e 119 um 18 1150 11 e 120 C calibrating monitors 44 calibrating monitors for KTAdesign systems 46 changing 63 chromatography systems type selection 50 component adding or removing 51 computer specifications 85 connecting chromatography system to remote and local computers 41 connecting system controllers to chromatography system 40 connection establishing 40 instrument sticks on scanning 84 limit 80 modes 41 not available 80 security 1 control capacity 86 control unit number selection 51 creating user groups and users 14 curve settings 77 system settings instructions 118 data buffers 86 buffers capacity 86 minimising loss in the event of communication failure 86 security 2 transfer 86 date and time correction 83 demo system 30 dialogue Component 52 Create New User 59 Enter Informati
96. rd Settings for CU 900 and UNICORN Control Board AT expansion cards Two settings that are made on the expansion cards are for inter rupt IRQ and address These settings must be entered in the UNI CORN software installation so that UNICORN can find the systems when started To see which settings the card has look at the card and compare it with the tables below The IRQ is set by a jumper and the address is set with a dip switch Write down the settings and set aside for future use Settings for CU 900 PCI expansion cards Set the control unit CU on the expansion card This setting must be entered in the UNICORN software installation so that UNI CORN can find the systems when started The CU is set with a dip switch To see which CU that is set look at the expansion card and compare it with Figs 3 1 and 3 2 Write down the number of the CU that is set on a paper and set aside for future use When you are installing the UNICORN software and the setup program is asking for the card settings enter the correct value for the selected CU Write down the settings on a paper for future use Special for CU 900 PCI and Windows 2000 Windows 2000 can auto detect the new card when the system is booted and the Add new hardware wizard is started The infor mation used to install the CU 900 PCI card on Windows 200 Using the Add new hardware wizard is found at Cu900pci inf inthe folder Drivers Cu900pci on the CD ROM I
97. rk is assigned access to User setup Levels 4 2 6 Changing user attributes Users can change their user attributes from the Main Menu with Administration Change User Attributes command e p63 4 Administration Note Note an 18 1150 11 e p64 Change user attributes xj Toobars Variables Show unused TT Show details Cancel Help Fig 4 12 The Change user attributes dialogue 4 2 7 Viewing and changing user definitions To view the setup for any user click on the user name in the User setup dialogue To change the user definitions make changes in the Group menu as appropriate and click on OK 4 2 8 Deleting users To delete a user select the user from the username list and click on Delete You may delete all users except the last user with User setup Levels access This ensures that at least one user has the right to perform administration functions Deleting a user does not delete the user s home folder or method and result files 4 2 9 Deleting folders To delete folders select a user in User Setup and then click on Access Folders in the User properties dialogue Select a folder s to delete in the right hand side of the dialogue that is displayed and click on Delete Click on Yes in the pop up dialogue to confirm the delete action You cannot delete a folder to which a user has access either as home folder or as a shared folder A deleted file cannot be recovered wit
98. rmation 4 3 Audit trails 0 0 ccc ee 4 3 1 Examining audit trails 0 00 00 c cee 4 3 2 Renewing audit trail files 000 000 4 3 3 Backing up audit trail files 000 0000 4 4 Report Generator Wizard cece ees 4 4 1 Generating a report from the Main Menu 4 4 2 Generating a report from System Control 5 System settings bi EL HA 5 2 CUrvess sa BNG RIN NU eda Sie ha Se eS 6 Troubleshooting 6 1 Logon problems LL 6 1 1 Unable to log on to UNICORN 000 6 1 2 Error message Strategy file error 6 2 UNICORN access problems 6 2 1 Unable to access certain UNICORN functions 6 2 2 Connections are not available 6 2 3 Run data Connection in System Control displays a No x 6 3 Method and run problems annann 81 6 3 1 Cannot Quit or Logoff from UNICORN 81 6 3 2 Monitor signals do not appear in the System GontrolCurves panel Liar he ta ee be nee oes 81 6 3 3 Error message Couldn t create result file 81 6 3 4 The Method System Connection dialogue keeps appearing 81 6 3 5 The Method Editor window does not fit on the screen 82 6 3 6 There are red instructions in a method 82 6 3 7 I ve logged out of Windows NT 2000 and then logged in again but can not get s
99. rned cannot be displayed in the curves window during a process run and will not be recorded in any way an 18 1150 11 e p78 Troubleshooting Gi 6 Troubleshooting 6 1 Logon problems 6 1 1 Unable to log on to UNICORN Choose your username from the list and enter your password If you have forgotten your password ask the system administrator for a new one If you cannot log on using your correct username and password the USERS30 MPM file in the UNICORNISERVERVIIL folder may be corrupt Restore the file from the latest back up copy or reinstall the default user If users are not available on a remote station in a network installation the user list in the Logon dialogue box is empty make sure that the computer is logged on to the network before starting UNICORN A remote station accesses the user list directly from the network server If the user list on a local station in a network installation is not up to date make sure that the computer is logged on to the network before starting UNICORN The user list is stored locally on a local station and is updated automatically from the network server if the computer is logged on to the network 6 1 2 Error message Strategy file error If you receive the error message Strategy file error Can t load strategy in a stand alone installation the strategy file is probably corrupt Reinstall the strategy as described in Installing selected software components after the ini
100. romatography systems may be controlled without running the user interface modules In a network installation this situation can arise if a system is controlled from a remote station without starting UNICORN on the local station In a stand alone installation the situation can arise if a user quits UNICORN after starting a run In both cases it is not apparent from the desktop that UNICORN control software is actually running and there is therefore a risk that Windows NT may be shut down and the computer turned off in the belief that it is not in use To prevent this Do not quit UNICORN if you are controlling a system Do not turn off local station computers in a network installation If possible start UNICORN application program on all local stations in a network installation and establish a view mode connection as an indication that a connected system might be running ep3 ED Security features an 18 1150 11 e p4 Network setup Gi 2 Network setup UNICORN 4 0 can be installed in a network environment allowing chromatography systems to be controlled from any PC in the network where UNICORN is installed This chapter describes how to set up the network environment before installing UNICORN This chapter is only relevant if you will be running UNICORN to control chromatography systems remotely The Network setup should be performed by someone with experience in Windows NT Windows 2000 and network installations 2 1 In
101. s are intact If those actions do not help try to restart both computer and system 6 5 2 Flowscheme If the flowscheme is not displayed properly check that you have selected 65536 colours under Windows NT 2000 Start Settings Control Panel Display Settings Technical specifications co A Technical specifications A 1 System requirements A 1 1 Hardware requirements Windows NT 4 Compaq PC Pentium II 333 MHz or higher minimum Pentium 200 MHz Windows 2000 Compaq PC Pentium III 500 MHz or higher 128Mb RAM for one system 256 Mb RAM for two or more systems 150 Mb available hard disk space Colour monitor 1024x768 pixels small fonts 64k colours 1 ISA or PCI slot per connected system CD ROM drive 1 44 Mb 3 5 diskette drive Mouse Supported printers HP DeskJet 660C HP DeskJet 895C HP DeskJet 950C HP LaserJet SMP A 1 2 Software requirements Microsoft Windows NT Workstation 4 0 with Service Pack 6 or later or Microsoft Windows 2000 A 1 3 Network requirements These are the recommended network requirements for running UNICORN in a network installation Supported Network cards 3COM Etherlink III Compaq Netelligent 10 100 TX Embedded UTP Controller Compaq Integrated NetFlex 3 Controller AMD PCNET PCI Ethernet Adapter Integrated Novell NetWare version 4 50 189 or later or Microsoft Windows NT Server 4 0 The UNICORN software works on earlier versions as well even though some versions of the No
102. s such that folders can be connected to a drive unit The two last points are satisfied if the services Server and Workstation are installed and one of the protocols TCP IP or NWLink IPX SPX User rights The requirements in the user rights list above are satisfied if a default installation of the network is performed The default is for everyone to have the user right Access this computer from network However if this is nor the case then the user rights should be set by a person with administrative privileges on the Windows NT 2000 workstation using the following steps 1 Select Start Programs Administrative Tools Common User Manager 2 Select Policies User rights from the dialogue menu 3 Select Access this computer from network from the drop down list 4 Click on the Add button 5 Select the group or person s who will get this right It should be a group within which the person logged on to the local UNICORN station is a member or that person is specifically selected 6 Click on the OK button 2 3 Installation guide 2 3 1 TCP IP Windows NT 2000 domain This is an example of how to setup the network so that UNICORN may run across the network when the network protocol is TCP IP and there is an Windows NT 2000 domain controller The setup of the Windows NT 2000 domain controller is not described here a competent network administrator is generally needed to setup an Windows NT 2000 domain server Network setu
103. s will be read Click on Continue The bindings are displayed Click on Next to continue Click on Next to start the network On the primary Domain Controller run Server Manager by selecting Start Programs Administrative Tools Server Manager From the menu select Computer and then the Add to Domain item Select the Windows NT 2000 or Server radio button and enter the Windows NT 2000 workstation name in the Computer Name field epll 2 Network setup an 18 1150 11 e p12 20 21 22 23 Click on Add The computer is added to the domain On the Windows NT 2000 workstation select the Domain radio button and enter the name for the domain The network administrator should supply this name Click on Next to continue You will be welcomed to the domain Acknowledge and click on Finish Select the option to restart your computer now The computer is restarted Log on to the Domain with administrator rights Installing TCP IP on a networked computer This is relevant when network has been installed earlier 1 2 10 11 Log on to the computer with administrator rights Open the control panel with Start Settings Control Panel Double click on the Network icon In the Network dialogue enter the Adapters tab and click on the Add button In the dialogue that is displayed select the correct driver for your network adapter and click on the OK button Click on the Have disk button and insert the disk
104. sabled Ena bled Alarm_AirSensor2 Sets the alarm for AirSensor2 An alarm will set the system in Pause if air is detected in air sensor flow cell 2 Mode Disabled Ena bled Alarm_AirSensor3 Sets the alarm for AirSensor3 An alarm will set the system in Pause if air is detected in air sensor flow cell 3 Mode Disabled Ena bled SetCondScale0 Sets the 0 scale of the relative conductivity signal The relative conductivity is displayed as a per centage of bufferB in run data Cond and as a curve Cond CondValue 0 999 mS cm e p101 Strategy for AKTAexplorer 100 an 18 1150 11 e p102 SetCondScale 100 Sets the 100 scale of the relative conductivity signal The relative conductivity is displayed as a per centage of bufferB in run data Cond and as a curve Cond96 CondValue 0 999 mS cm WatchPar_UV1 The Delta_Peak setting affects only the Less_than_or_valley and Peak_max_conditions when using the Watch instruction see UNI CORN User Manual As a gen eral guideline set the value to 2 3 times the noise level and 5 10 of the smallest expected peak height Delta_Base setting affects only the Stable_baseline condition For this condition to be activated the sig nal may not vary by more than the Delta_Base value up or down over the time interval specified in the Stable_baseline condition in the Watch instruction Delta_Peak 0 6000 mAU
105. shed click on the Flowpath radio button Mark instruction ColumnPosition if available and ensure that Position1Bypass is selected 4 Click on the Pump radio button and select the instruction Flow Insert the flow rate that you will use later during your run and click on Execute Installation EB 5 Select System Calibrate and select monitor pH 6 The Measured value field shows the actual reading according to the previous calibration This value may be incorrect and does not affect the current calibration Allow at least 35 ml of reference buffer to pass through the cell during which time the reading should stabilise 7 When the pH is stable enter the known pH of the reference solution in the field for Reference value 1 Click on the Read Value 1 button 8 Switch to the second reference solution by selecting Manual Pump and the instruction PumpWash Select Inlet B and click on Execute When the PumpWash is finished select the instruction Gradient set target to 100 B after 0 minutes and click on Execute Repeat steps 6 and 7 for Reference value 2 9 After the calibration values are automatically entered into the Calibrated electrode slope and Asymmetry potential at pH 7 fields A new electrode will have a slope between 95 102 and an asymmetry potential from 30 to 30 mV Should the values fall outside these ranges clean the electrode If the slope falls below 80 and the asymmetry potential outside 60 mV and cannot b
106. t is important for system security that this user is deleted when the site specific users have been created 1 2 Connection security To prevent conflicts in System Control requests each system in an installation may have only one control mode connection at any one time A running system may be left in a view mode connection and locked with a password independent of the user s logon password to prevent users without access from establishing a control mode connection and interfering with the control of the separation process Otherwise if the system is left unlocked in a view mode connection any user may establish a control mode connection to the system e pl n Security features Note an 18 1150 11 e p2 We recommend that systems are always locked when a user leaves the system For controlled and locked systems the responsible user is identified in the System Control window for view mode connections A system which is left unlocked with no control mode connection has no identified responsible user Systems may be locked even when they are idle to allow users to reserve a system for later use 1 3 Data security 1 3 1 Network communication failure If the network communication fails while a method is running any control mode connection on a remote station will lose control of the system and results destined for network drives cannot be saved in their correct folders In this event the run continues under control from
107. talled on the system You must select the strategy to which the method template files correspond Templates together with wizards are installed on the network server in a network installation Manuals Check the Manuals option to re install the Manuals This will not affect any existing method or result files in the system The manuals are installed locally even in network installations 3 5 Managing system connections UNICORN installed on a given computer may have up to four System Control windows the actual number is determined when the software is installed each of which may be connected to one chromatography system at a time Connections are managed using the Connect and Disconnect commands in the System menu A network installation may have more than four systems in total but each computer in the network can establish a maximum of four connections Connection management is the same for stand alone and network installations 3 5 1 Establishing a connection To connect a System Control module to a chromatography system open a System Control that is not currently in use identified by the Not connected text in the status bar and choose System Connect or VA click on the System Connect toolbar icon The dialogue lists the systems to which you have access Select the system to which you want to connect and click on OK System Connect Ea Basic10 Explorer10 OK Cancel Help Fig 3 19 System connect dialogue an
108. tationary files dll and exe for the system An integrity check involves a new checksum calculation being performed for the same files in their folders and comparing the new calculated value with the checksum value obtained during installation The results of the comparison are presented in the report and any deviations are included Generate Report The Generate Report dialogue displays a tree structure of all drives that are reached from the computer performing the report generation By default the report is saved in the folder Unicorn Reports although you can change this folder to another one of your choice Generate report Select a directory to store the report and press the Finish button to create it CJ TEMP a Unicom Q Bin Q DI H E Local EI J Report 98011401 Report 38020801 CI Server Settings H E WINNT4 ed E S3 gary on pit 500 H PA Kali a ENO EY le pa reen crea teo You will also notice that the Print and Preview buttons are enabled in this dialogue Preview opens the report in NotePad while Print prints out the report without any preview Generating a report from System Control If you activate the Generate Report Wizard by clicking on the Report button in an error message dialogue in System Control the following dialogues are displayed ep 3 gt Administration an 18 1150 11 e p74 Generate Report Wizard See description above Description All of
109. tem Fig 2 2 General network organisation based on a Novell network controller The setup of the Novell server is not described here a competent network administrator is normally needed to setup a Novell server Creating user group and users of UNICORN on the Novell server This is performed by the network administrator The network administrator creates the users that will be running UNICORN on the Novell server The procedure for this is not described here Sharing a folder This is performed by the network administrator The network administrator creates a shared folder on the Novell server which UNICORN will use for shared data The procedure for this is not described here Creating user group and users of UNICORN on the local computers 1 Logonto the client computer with administrator rights 2 Enter the User Manager with Start Programs Administrative Tools Common User Manager 3 5 6 Network setup Gi Create the users that will run UNICORN Select the User menu and the New User item In the dialogue that is displayed enter the user name the UNICORN users created by the network administrator description and password enter the password the user has in the Novell network Make sure that the Password Never Expires option is checked and that the User Must Change Password at Next Logon option is not checked Click on OK in the New User dialogue and the user is created When completed close User Manage
110. tem System 1 Control Board CU 900 UNICORN Control Board AT C CU 900 PCI Hep Fig 3 13 System Setup dialogue an 18 1150 11 e p34 Installation EB Select the type of control board that the system being installed is using CU 900 CU 900 PCI or UNICORN control board AT For each system you will have to set the correct settings for the control unit Additionally the control interrupt and address have to be set for CU 900 and UNICORN control board AT control board types the CU 900 PCI control board handles these settings automatically Refer to Hardware installation on page 19 for the correct settings The Default button gives the default resource settings for the selected system type If you want to remove a system click on the Clear button to clear all fields All fields must contain a value before you can continue Click on OK to save the changes or Cancel to abort the dialogue In both cases you are returned to the System Installation dialogue Install additional systems until you have installed all systems physically connected to the computer the number of systems is not related to the number of System Control windows installed in step 12 If you installed more than one system make a note of which system is connected to which control unit This information is useful to have when you set up the system table or if you must in the future reinstall UNICORN Note If you want to define syste
111. the local station when the network is not running Security features CE Grant access to the Failed folder only to one or a few users who share the responsibility for retrieving results files and deleting old files from the Failed folder This policy should be used if the installation requires restricted access to users result files Note that the user s with access to the Failed folder should also have access to other users home folders to be able to copy or move result files to suitable destinations In granting access to the Failed folder it is sufficient to grant access to DRIVE Unicorn Local Fil Failed since this is the path to the Failed folder on each local station 1 3 2 Local station failure If the local station fails during a run the run may continue but the results generated after the failure cannot be saved An autosave feature saves a temporary result file on the local station during runs at a pre set interval defined in the system definition see System definitions on page 49 When the local station is restarted the temporary result file will be transferred to the original result file destination or to the Failed folder if the original destination was on a network drive which is not currently available The result file will contain the results of the run up to and including the last autosave time before the failure Results after this time will be lost 1 4 Security recommendations for control stations Ch
112. the problems errors that are currently listed in the System Control error message dialogue are recorded here Help text is then shown specific to the selected error in the error message dialogue By selecting a different error in the dialogue the appropriate text is shown All problems with the help texts are included in the report You also have the possibility to enter a short description of the problem the circumstances under which the problem occurs and the consequences caused by the problem Reproducibility See description above Attachments See description above One exception is that log book files can be attached instead of result files Generate Report See description above Note System settings E 5 System settings Fach installed system has a set of default settings for example to define system settings for alarms and warnings and to select which data will be stored in result files The default system settings are valid for all runs unless you change the settings in one of two ways you assign a new value to a parameter within a method The specific change that you make in the method is valid only until End After End the parameter returns to its default setting See Appendix B in the UNICORN 4 0 User Manual and Appendix B in this manual for settings defined in AKTAexplorer 100 system and other UNICORN System Controller strategies respectively you assign a new value to the actual system setting This new va
113. the set flow rate The sample pump can be calibrated in System Control Calibrate SamplePump 0 50 ml min e p93 Strategy for AKTAexplorer 100 an 18 1150 11 e p94 DirectLoad The sample is directly loaded onto 7 SampleFlow the column ReduceFlow makes it 0 50 ml min possible to automatically reduce the Volume flow during sample loading to avoid 0 20000 ml a pressure alarm An alarm will be ReduceFlow received if the flow is lower than the No Yes flow specified in MinFlow If an MinFlow alarm is executed and the run is con 0 1 49 9 ml min tinued by pressing Continue the sample loading will be stopped and the run will continue PumpWash PumpWash is used to change eluents InletA1 Explorer in the inlet tubings pump and OFF A11 A18 mixer THE PUMPS MUST BE InletA2 PURGED PRIOR TO USING OFF ON PUMPWASH Up to four inlets can InletB1 be washed simultaneously InletA1 OFF ON InletA2 InletB1 and or Inlet B2 The InletB2 system is set to Pause and the injec OFF ON tion valve is set to Waste during PumpWash After completed wash the injection valve is turned back to its previous position and the flow rate continues with previously set value Flow rate 25 ml min and Wash vol ume 30 ml inlet PumpWash PumpWash is used to change eluents InletA1 Purifier in inlet tubings pump and mixer OFF ON THE PUMPS MUST BE PURGED InletA2 PRIOR TO USING PUMPWASH OFF ON Up to fo
114. thod templates global procedures global report formats global columns or global BufferPrep recipes also global Quantitation tables and global Mol Size tables We recommend that this authorisation is restricted to only one user in an installation e Maintenance Required to gain access to the System Maintenance command in System Control e Quit program Required for ending a UNICORN session with the File Quit program command in the Main Menu e Service mode This mode is for Amersham Biosciences service personnel only 4 2 2 Access group examples Below are some examples illustrating the way groups of users can be assigned differing access levels in a multi user installation UNICORN is installed with a number of predefined Access groups that can be changed to the requirements of each individual group System administrator The administrator has special responsibility for maintaining user system and audit information and for file management in the PC folder structure backup routines etc The administrator may not edit or run methods or MethodQueues issue manual instructions calibrate monitors or change the system configuration Administration ci Caution We recommend that only one user in an installation or network is assigned system administrator rights If several users can change user definitions and system connections confusion can rapidly follow User properties xj Access groups r Access
115. tial installation on page 39 In a network installation the error may appear if you try to create a method for a system not physically connected to the computer Make sure that the computer is logged on to the network before UNICORN is started so that the strategy file on the server disk is accessible 6 2 UNICORN access problems 6 2 1 Unable to access certain UNICORN functions UNICORN functions to which you do not have access appear grey in the menu and cannot be used Your user profile is determined by the system administrator from Administration User Setup in the Main Menu e p79 HD Troubleshooting an 18 1150 11 e p80 6 2 2 Connections are not available Check the connection between the PC and the chromatography system Check that the power to the chromatography system is turned on If the connection appears to be correct and the power is turned on switch off the chromatography system and quit UNICORN Shut down and switch off the computer then restart the entire system If a system is not available when you attempt to establish a connection check that you have access rights to the system Access rights are not automatically assigned for a newly defined system If you receive the error message Cannot connect to system in a network installation check e that the local computer to which the system is connected is turned on and logged on to the network e that the computer from which you are trying to esta
116. time fee for this Amersham Pharmacia Biotech AB software product the customer receives from Amersham Pharmacia Biotech AB a licence to use the product subject to the following terms and conditions zl Do you accept allthe terms of the preceding License Agreement If you choose No Setup will close To install UNICORN you must accept this agreement lt Back Yes No Fig 3 4 UNICORN Software License Agreement dialogue 7 Enter your name company and product serial number for the software Click on the Next button to continue User Information Type your name below You must also type the name of the company you work for and the product serial number Name Amersham Pharmacia Company Boech OS ial XXXXXXXXXXXXKXX Serial Fig 3 5 User information dialogue e p27 ED Installation Note The serial number can be found on the UNICORN License Agreement that is shipped with the CD 8 Select the components to install The components User information and Strategy and template files contain sub components These sub components can be accessed by clicking on the Change button When all selections are made click on the Next button to continue Select Components X Select the components you want to install clear the components you do not want to install Components Program Files User Information 1632K Strategy and Template files OK System Installation 48K Manuals 7860K Description
117. troduction It is important to understand the basics of how UNICORN operates in a network setup to ease the installation The first step is to login to a network to enable installation of UNICORN Then read the following explanations of some of the aspects of UNICORN in a network setup Explanation of some of the aspects of UNICORN in a network Storage of data Communication Log files in a net work setup Named pipes com munication Network failure in the middle of a run Access to the net work drive while running Server ina UNI CORN network setup Methods and log files are stored in a folder shared between the local and the remote UNICORN The remote and the local UNICORN use named pipes to send commands and data between them While running the local UNICORN system writes logs on the local harddisk When the run is over it copies them to the network drive where log files are stored From the remote UNICORN commands are sent such as run method foo met From the local UNI CORN messages and trend data is sent to the remote UNICORN The local UNICORN will continue the run and the log file will be on the local harddrive when the run is over When a method is begun the method is copied from the network drive to a local directory and it is read from there during the run UNICORN requires only a directory for log files and method templates accessible by both the local and the remote UNICORN It is generall
118. ts all connected systems displaying the Leave control dialogue for each system Systems which are disconnected in this way will be re connected automatically when you log on to UNICORN again Note however that you may have disconnected from a control mode connection but establish a view mode connection on re connect if another user has taken control of the system in the meantime e p43 ww Installation Note an 18 1150 11 e p44 You can disconnect a system during a run and the run will continue It is not recommended to do this without locking the system since this can leave a run on the system with no responsible user You cannot however disconnect from scouting or MethodQueue runs 3 5 5 Network considerations In a network installation a chromatography system can be controlled from any computer in the network provided that the user has sufficient access rights in UNICORN UNICORN software has to be installed but not necessarily running on the computer to which the system is physically connected The computer has to be logged on to the network e A system which is controlled by another user through a control mode connection can be viewed through a view mode connection by any user with sufficient access on any number of computers in the network This allows runs to be monitored from multiple display stations although only the active connection can control the system e A system that is locked by a user can be unlocked o
119. uctivity e p91 B Feedback tuning an 18 1150 11 e p92 Strategy for AKTAexplorer 100 C Strategy for AKTAexplorer 100 A dedicated strategy is available for each system in the AKT Adesign platform The instructions available differ slightly between strategies although the majority are common To find a description of any instruction in your particular strategy mark the instruction and click on lt F1 gt To obtain a printout of the instructions select File Print in the Method Editor and check the Instruction set option As an example this appendix lists the instructions for methods manual control and system settings supported by the strategy for AKTAexplorer 100 E100f300 C 1 Method instructions C 1 1 Pump Instruction Description Parameters Flow Starts the flow for the system pump Flow rate at the set flow rate 0 100 ml min Gradient Defines a linear gradient for gradi Target ent pump pump A and B Reaches 0 10096B the target value after the specified Length length Length is expressed in time volume or column volume depend ing on selected base Base is selected in Methodologies Base in Manual is always time 0 99999 base BufferPrep_pH Selects pH for a defined BufferPrep pH recipe When BufferPrep is 0 14 changed to OFF in a method the instruction should be set to zero otherwise an alarm will be given SampleFlow Starts the flow on the sample pump Flow rate at
120. ue 4 1 1 Defining new systems To define a new system click on New in the System Setup dialogue The New System dialogue is displayed 1 Enter the system name in the System name field The system name can be set only when defining a new connection and cannot later be edited since user access rights are linked directly to the system name Names can be up to 30 characters long 2 Select a System type either Chromatography or Oligo 3 Select a strategy for the system from the pull down list in the Strategy field Available strategies are determined when UNICORN is installed see Hardware installation on page 19 If you have several strategies installed make sure that the selected strategy is appropriate for the chromatography system being defined Click on the Information button to display information about the selected strategy 4 The Pipe server name field is the same as the Windows NT 2000 computer name Leave this value unchanged Note Administration ci 5 Select the control unit number 1 4 in Control unit number This is the physical connection number for the chromatography system on the local computer see the section Installation on page 19 6 Entera value in the Autosave interval field if you want UNICORN to save a copy of the result file at pre set intervals during a run This minimises loss of data in the event of a computer failure The recommended interval for most systems is 5 minutes A shorte
121. ul ume is the amount of sample or trans FlushVol port liquid taken from a vial before 30 100 ul the loop is filled with sample Head 787 WashVolume space ensures that no air or vapour 300 9999 ul bubbles are formed during sample TrayType aspiration Only one type of tray seg StandardVial A ment can be used at a time MicroVial D HeadPress No Yes NeedleHght 0 40 mm Autosampler Sets the speed of the syringe The Speed SyringeSpeed speed is dependent of the scaling Low Normal parameter but also of the syringe vol High ume See table in A 900 User Manual Scaling If the samples are highly viscous it 0 1 1 0 may be necessary to program larger flush volumes and reduce the syringe speed for better performance Strategy for AKTAexplorer 100 AutoSampler_ Sets the temperature for the autosam Temp Temperature pler Maximum temperature decrease OFF 0 will be 12 C below the surrounding 0 C 4 40 temperature FracParameters Parameter settings for fraction collec DelayVol tor Delay volume is defined as the 0 10 ml volume from UV cell to the end of the TubeChange tubing for the fraction collector Col Tube DropSync lection of flow during tube change can Accumulator be handled in different ways Accumu lator The flow will be collected in the accumulator during tube change and then be delivered in the next tube DropSync Tube change synchronised to drop release S
122. ur inlets can be washed InletB1 simultaneously InletA1 InletA2 OFF ON InletB1 and or Inlet B2 The system InletB2 is set to Pause and the injection OFF ON valve is set to Waste during Pump Wash After completed wash the injection valve is turned back to its previous position and the flow rate continues with previously set value Flow rate 25 ml min and Wash volume 30 ml pump PumpWashBasic Strategy for AKTAexplorer 100 Pump Wash is used to change eluents in the inlet tubings pump and mixer THE PUMPS MUST BE PURGED PRIOR TO USING PUMPWASH Both pumps can be washed simultaneously PumpA and or B The system is set to Pause and the injection valve is set to Waste during PumpWash After completed wash the injection valve is turned back to its previous posi tion and the flow rate continues with previously set value Flow rate 25 ml min and Wash volume 30 ml pump PumpA OFF ON PumpB OFF ON SystemWash System Wash will change eluents in the system The InletA1 used during the SystemWash will be the same as chosen before the SystemWash THE PUMPS MUST BE PURGED PRIOR TO USING SYSTEM WASH The system is set to pause ColumnPosition to Position1Bypass and flow is diverted to waste of the outlet valve during SystemWash The valves and the flow rate will return to their previous settings after SystemWash In BufferPrep mode SystemWash delivers buffer with the selected composition Re
123. vell Netware driver have known problems A valid network connection e p85 A Technical specifications an 18 1150 11 p86 A 2 Control capacity A 2 1 Stand alone installations Simultaneous control of up to four chromatography systems Each module is separately configured in a system strategy supplied by Amersham Biosciences A 2 2 Network installations A few basic facts about a network installation e Chromatography systems must be locally linked to a workstation and then the workstation is linked to the network i e the chromatography systems are not directly linked up to the network e Each local workstation can be connected to four separate chromatography systems e A network can support up to 99 chromatography systems which are connected locally to the workstations in the network e A workstation can locally or remotely actively control up to four chromatography systems This is achieved using the four possible System Control windows in UNICORN available on each workstation e Each chromatography system may be controlled by only one active System Control window and be viewed by seven different other System Control windows in UNICORN A 3 Data sampling Data from chromatography system monitors are stored temporarily in data buffers in the local system controller Data are transferred from the buffers to disk storage by UNICORN whenever a chromatogram is closed i e when the New_Chromatogram instruction
124. ver Controlled user access to security back fand local disks home and shared folders e g up routines placing all home folders on a shared disk to prevent data from being scattered through lout the network Network access Maintenance of user pass security words and access rights to shared resources UNICORN access security Maintenance of user profiles Note Note Network setup Gi PC s with UNICORN software can be categorised as follows e A local station is a PC to which chromatography systems are physically connected UNICORN software must be installed on all local stations in the network e A remote station is a PC to which often no systems are physically connected but which can control systems over a network link UNICORN software is installed with the Remote Only option on remote stations Chromatography systems in the network can be controlled from either remote or local stations A local station in a network can also be used as a remote station to control other systems If a run is being controlled from a remote station and a network communication error occurs the run will continue under the control of the local station Results will be saved in the Failed folder on the local station A control mode connection can be established on the local station to control the run For a chromatography system to be accessible in the network the local station must be switched on and logged on t
125. y a good idea to use a directory on anWindows NT 2000 server for easy backup ep5 2 Network setup Note an 18 1150 11 ep6 The different network setups that UNICORN has been tested in are the following e Windows NT 2000 domain with TCP IP e Windows NT 2000 workstations connected to a Novell server The same version of UNICORN must be installed on both the computers with the chromatography system and the remote control computers Mixing different versions will not work Establishing and maintaining a networked UNICORN installation requires some understanding of the working of the Windows NT 2000 operating system and the concept of NT domains if used We recommend that a competent network administrator that will also be involved in the installation of UNICORN software should maintain the network The network administrator is not necessarily the same person as the system administrator for UNICORN Once the network is set up network functions are entirely transparent to UNICORN users and the network administrator does not need to understand the use of UNICORN for controlling chromatography systems Security is very important in a networked UNICORN installation Three aspects of security can be distinguished and the responsibility for maintaining security is shared by the network administrator and the system administrator for UNICORN Network administrator UNICORN administrator Data storage Back up routines for ser
126. y transporting WashExt the programmed injection volume into No Yes the sample loop The sample loopis AirSegment partially filled with sample After NoAir Air every injection the needle is rinsed and if WashExt is programmed the outside of the needle is washed with WashVol settings An air segment is used to reduce the amount of the flush volume and will not be injected an 18 1150 11 e p96 Strategy for AKTAexplorer 100 Injection Selects the injection mode Injection VialNo Flushed Flushed for the AutoSampler The 1 160 syringe dispenser fills the sample line WashExt with sample FlushVol settings The No Yes valve turns and the sample loop is AirSegment filled with sample The sample loop is NoAir Air filled two or more times the loop vol WashExt ume depending on the volume of the No Yes loop The loop volume is selected in system settings 100 ul is the standard loop volume After every injection the needle is rinsed and if WashExt is programmed the outside of the needle is washed with WashVol InjectionPickup Selects the injection mode Injection VialNo Pickup for the autosampler The 1 160 syringe dispenser fills the sample line 8 TrpViaINo with liquid from transport vial The 1 160 needle moves from the transport vial Inj Volume to the sample vial and picks up the 1 475 ul programmed injection volume The WashExt needle moves back to the transport
127. ystem Note 2 Changes made in the selection of the components are copied to the audit trail 4 1 2 Editing system definitions To edit an existing system select the system in the System setup dialogue and click on Edit Alter the parameters as appropriate Remember that if you change the system strategy the arrangement of tubing pumps columns etc may need to be changed on the chromatography system An attempt to control a system using the wrong strategy may cause malfunction and damage to the chromatography system 4 1 3 Deleting system definitions To delete a system definition select the system s in the System Setup dialogue and click on Delete A system definition can only be deleted if the system is idle and no users are connected to the system an 18 1150 11 e p52 Administration ci _ 414 Deleting strategy definitions To delete a strategy definition select a system in the System Setup dialogue and click on Strategy Select a strategy to delete in the Strategy Administration dialogue and click on Delete Strategy Administration x Strategy Strategy used on system B100F200 Delete Help Fig 4 4 The Strategy Administration dialogue 4 2 User administration Access to UNICORN software is controlled by username and password authorisation Within the program each authorised user is assigned to an access group that determines functions the user can perform From the User Setup dialogue it is
128. ystem connection in UNICORN only for local systems not remote 83 6 3 8 Print screen does not send a copy of the screen to The Printers reeeo neri iaia Ladin Lisa ta 83 6 4 Evaluation problems cece eee eee 83 6 4 1 Incorrect date and time 83 6 4 2 Evaluation procedure aborts 0 0000 cee eee 83 6 5 AKTAdesign system specific problems 84 6 5 1 Connected to a system but no system contact 84 6 5 2 1FIOWSCHEMC ie cera aule ae ian 84 A Technical specifications A 1 System requirements i 85 A 1 1 Hardware requirements 85 A 1 2 Software requirements 85 A 1 3 Network requirements 85 A 2 Control capacity i 86 A 2 1 Stand alone installations 86 A 2 2 Network installations 86 A 3 Data sampling eee 86 B Feedback tuning B 1 Flow rate tuning i 90 B 2 Gradient tuning ccc cee ees 91 C Strategy for AKTAexplorer 100 C 1 Method instructions 0 00 cc eee eee ee ees 93 Gal NG RUIN Res gt ein ke eae Neds at cates Bee 93 CE2 FIOW Path i essre Moio i SoU ate oa oll Landen as 96 Gis AlarmsS amp Monitors 0 58 8 Ae Lie Mee Pens Bee Sally 99 GPA Failed iii cai 105 GIO Wate stenti il e Ne 107 C6 FOIE atti I era 108 COME nen Pin the gl ta TGA e a 108 C 2 Manual control cee ee 110 Ci2 1 PUM 22 tes NAL i NG LI GG Ako E 110 C22 Fl0W path aa aT NAG ka BN i alti al 110 C 2 3 AlarmsS amp

UNICORN Version 4.0 Administration and Technical Manual

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