USER`S MANUAL - Frank`s Hospital Workshop
Contents
1. 3 0 82 3 5 1 CRYSTAL VIOLET C 35 4 5 43 55 2 WASH D 25 17 3 34 23 5 3 IODINE B 30 4 5 38 5 7 4 WASH D 33 22 8 69 47 8 5 DECOLORIZER A 39 6 0 47 7 2 6 WASH D 20 13 8 34 23 5 7 HIGH SPEED DRY 55 55 DURE 237 320 Total without alcohol fixation re 69 a 113 3 ee 307 402 Total with alcohol fixation Baran 144 6 7 min 116 8 Option for ethanol or methanol fixation References PRODUCTS REFERENCES PREVI Color Gram CD Rom PREVI Color Gram User s Manual PREVI Color Cytocentrifuge Rotor User s Manual General Safety and Regulatory Information e fr en de es it pt el sv da pl hu sk e ct It Iv ru ja tr na zh ko th REAGENTS e Acetone safranin A e Safranin A e Acetone fuchsin A e Fuchsin A e lodine B See your e Crystal violet C bioM rieux Nozzles 2 representative Nozzle o ring Nozzle wire Nozzle cleaning solution Slide carousel 12 30 D amp E empty bottle D amp E 5 Liter empty bottle Waste tubing Waste container Maintenance kit PREVI Color Gram User s Manual 2 3 General Description Description The PREVI Color Gram consists of LA aa with safety lock e Right side panel with 5 prime buttons for 5 Carousel 30 reagent channels or Carousel 12 Fuchsin or safranin with or without acetone Bowl see page 2 5 lodine Front panel see page 2 4 Crystal Violet Distilled water Ethanol or methanol Front panel and keypad description Indicato2. e fast staining e high volume staining productivity 12 or 30 slides at a time e CLEAN cycle to purge each spray nozzle with ethanol or methanol e 9 operator selected decolorizer cycles e separate reservoir delivery tube pump and spray nozzle for each reagent e operator selectable ethanol or methanol fixation function to fix samples The correct accessory must be used for each function The PREVI Color Cytocentrifuge Rotor is available from bioM rieux as an option offering additional features program no 2 and 3 recommended by bioM rieux The PREVI Color Gram is an in vitro diagnostic medical device for professional use only The PREVI Color Gram has been designed to perform automated Gram staining of human sample micro organisms for in vitro diagnostics with bioM rieux reagents only The PREVI Color Gram has been qualified with the following cell suspensions e blood e bronchoalveolar liquid BAL e cerebrospinal fluid CSF e feces e sputum e vaginal swab e urine bioM rieux disclaims any responsibility for the use of the PREVI Color Gram with biological cell suspensions other than those mentioned above PREVI Color Gram User s Manual 2 1 General Specifications Specifications PARAMETERS CHARACTERISTICS Main power input 100 240 V 50 60 Hz The power setting is indicated on the back panel Power Consumption 85 W max Fucecatin 2A 250V IEC TYPE T g 5x20 mm 0 2x7 9 inch
3. EE 5 16 LOIMG tEMM SIONAG Siria een inde AR et en An TN et At Rain ten 5 16 a A A 5 18 Cleaning the instrument and the carousel sise 5 18 E 5 18 EVE AIO ESS a a a ee ei De tu 5 18 Disintecting and or stenliN Or ida 5 19 DISIN CIONSOMUIONS EE 5 19 DisiMtecuon Procede ita cai 5 20 IAStTUMentS AID IMO dansa RE 5 22 DiSiM CIOn e ee 1116 EE 5 22 FUso E e EE 5 23 6 Troubleshooting 6 1 Error messages and Troubleshooting Table 6 1 7 Preventive maintenance chart 7 1 Note Screenshots figures and messages are given for information purposes only 2 PREVI Color Gram User s Manual 1 How to use this manual Warnings This manual provides the appropriate instructions to install operate and maintain the PREVI Color Gram instrument Different types of warnings are used throughout the manual e for safety reasons DANGER e to ensure that the instruments are maintained in good working condition CAUTION e for regulatory reasons WARNING or e for optimum performance of operations procedures etc IMPORTANT Before using your instrument please also read the General safety and regulatory information booklet provided with the instrument and the above mentioned warnings Specific warnings A DANGER CAUTION The PREVI Color Gram must be installed in a well ventilated area If power is lost while the stainer is running do not open the lid until carousel rotation ceases Check the amber rota
4. Gram Monitoring reagent level Monitoring reagent level CAUTION The reagent and waste liquid levels must be monitored by the user To ensure proper staining and to protect the stainer never allow a reagent line to run dry When the reagent liquid level is near the bottom of a reagent bottle replace the bottle see Replacing a reagent bottle Ga page 4 12 DO NOT FILL ABOVE THIS LINE never allow the waste liquid level to go above the maximum safety level When the waste container is full empty it see Emptying the waste container page 4 13 IMPORTANT Do not transfer residual reagent from a used bottle to a fresh bottle Doing so can lead to noticeable accumulation of precipitates on the slide particularly with the iodine and Crystal Violet stains B amp C Replacing a reagent bottle Open the new bottle record the letter on the cap and keep it for future use e g long term storage P eg Unscrew the cap and remove the dip tube 7 from the empty bottle Insert the dip tube into the appropriate reagent bottle and screw on the ring cap O Discard the bottle in a biohazard container The instrument is ready for use 4 12 PREVI Color Gram User s Manual Operating the PREVI Color Gram Other settings Emptying the waste containe WEEKLY PROCESS Disconnect the waste tubing from the full waste container Empty the waste container ES Throw the effluents
5. and set the bottle aside O Flush at least 250 ml of ethanol or methanol through the line and spray the nozzle by priming Leave ethanol or methanol in the line Remove and clean the nozzles see Disassembly page 5 4 and Cleaning page 5 5 Flush the bowl with distilled water Q To operate the instrument again perform the previous steps in reverse order Maintenance and cleaning Long term storage IMPORTANT Leave flushing fluids in the reagent lines during storage CAUTION PREVI Color Gram User s Manual Do not run reagent lines dry Do not subject the instrument to freezing temperatures if aqueous fluids remain in any of the reagent lines Serious damage may result Maintenance and cleaning Cleaning Cleaning A DANGER Prior to cleaning disconnect the PREVI Color Gram from the main power supply to avoid any risk of fire and explosion The cleaning procedure should be performed in a well ventilated room by authorized trained personnel wearing appropriate individual protection equipment f e Cleaning the instrument and the carousel Clean the outside of the instrument and the carousel with a dry or moist cloth only If very dirty clean them with a cloth moistened with ethanol 70 or mild detergent For better cleaning leave the carousel for half a day in a container full of 10 mild detergent Rinse under water bioM rieux strongly recommends distilled w
6. prevent nozzle loss Inspect nozzle parts Remove any material in the swirl cone grooves by sliding a piece of paper along the edge Rinse parts in the tube with distilled water O Rinse with ethanol 70 Push the wire through the back of the disassembled nozzle Replace the O ring and apply silicon lubricant 5 6 PREVI Color Gram User s Manual Maintenance and cleaning Nozzle handling Reassemble the nozzle by inserting the swirl cone into the compression screw Hold all the parts ina vertical position until reassembly is completed o Return the nozzle to its original location Repeat instructions 1 to 8 for each nozzle Prime each reagent channel 10 Perform pattern and volume tests before reusing the instrument IMPORTANT If the test is still not correct see Unclogging the tubing page 5 8 PREVI Color Gram User s Manual 5 7 Maintenance and cleaning Nozzle handling Unclogging the tubing Remove the carousel from the stainer Remove the nozzle connected to the air locked pump Screw the nozzle adapter of the unclogging tool included in the nozzle maintenance kit into the nozzle holder Withdraw the unclogging tool plunger to create a vacuum Hold the plunger at midpoint to prevent it being drawn back by the vacuum Press the desired prime button to start the reagent pump 5 8 PREVI Color Gram User s
7. recurs please call your bioM rieux representative CAUTION Fuse failure may indicate a serious internal problem For continued protection replace only with fuses of the type and rating indicated on the rear panel of the stainer Put the power switch in position O Disconnect the power cord from the mains Disconnect the main power cord from the socket Open the cover with a small screwdriver Push the fuse holder to the left to check the fuses Replace fuses if necessary A Push the fuse holder to the right Close the cover o Reconnect the power cord socket and mains and switch on the instrument PREVI Color Gram User s Manual 5 23 6 Troubleshooting The following section provides suggestions to help you quickly solve routine problems that might be encountered with the PREVI Color Gram These solutions are indexed to additional information found throughout this manual Because more difficult problems may require detailed technical service institutions with qualified service personnel should obtain and refer to the PREVI Color Gram Service Manual Otherwise you should contact your bioM rieux representative for assistance The following table gives a brief description of the error messages and the troubleshooting actions IMPORTANT If the recovery procedure does not work please contact your bioM rieux representative Error messages and Troubleshoot
8. test M If the volume is in the tolerance range go to point 9 If the volume is still out of the tolerance range see Nozzle handling page 5 4 to clean the nozzle Repeat the tests with the cleaned nozzles v If the volume is in the tolerance range go to point 9 If the volume is still out of the tolerance range contact your bioM rieux representative PREVI Color Gram User s Manual 5 11 Maintenance and cleaning Volume test For future use empty the contents of the tubes into the bowl clean the tubes rinse the tubes with distilled water waste liquid thrown in the bowl put the tubes back into their original place in the main tenance box 10 e err 19JEM P3I1SI0 PREVI Color Gram User s Manual Maintenance and cleaning B line flushing procedure B line flushing procedure The maintenance kit is required for the following test that must be performed monthly B line is prone to corrosion and needs a higher level of cleaning IMPORTANT A carousel must be in place during the procedure otherwise it will generate an error and will not continue the process Remove the nozzle B Perform a B line flush by pressing O Load 500 mli of distilled water in the B line position and press Note pump approx 400 ml of distilled water through the B line PREVI Color Gram User s Manual 5 13 Maintenance and cleaning B line
9. the 12 slide carousel or 17 or more for the 30 slide carousel make certain you have not programmed the stainer for fewer slides If you program the stainer for a partial load be sure to load the slides in the correct positions as indicated by the markings on the carousel If the problem persists please contact your bioM rieux representative Make certain that both position magnets are still attached to the bottom of the carousel Make certain you have not programmed the stainer for fewer slides than you have loaded If you program the stainer for a partial load be sure to load the slides in the correct positions as indicated by the markings on the carousel Verify that each reagent pump is primed by opening the lid and pressing the manual prime button The nozzle should immediately spray a fine mist of reagent There should be no sputtering or hissing sounds to indicate the presence of air in the reagent lines If the problem persists please contact your bioM rieux representative Check the level of the Reagent D bottle Check Reagent D spray volume according to tolerance range Check nozzle spray pattern according to the procedures see Pattern and volume testing page 3 6 This type of discoloration is usually caused by a piece of debris or reagent precipitate clogging the spray nozzle orifice Clean any nozzle that exhibits a poor spray pattern If the problem persists please contact your bioM rieux repres
10. to protect the stainer interior Place the stainer in a biological safety hood or well ventilated area Spray the inner bowl and inner lid with a disinfection solution Repeat the spray treatment every 2 or 3 minutes Leave the solution on surfaces for approximately 20 minutes IMPORTANT For sporicidal disinfection allow the solution E to react for 10 hours Do not allow cleaning solutions to dry on the stainer surfaces Rinse the inner bowl and lid thoroughly with water bioM rieux strongly recommends distilled water 5 20 PREVI Color Gram User s Manual 6 Wipe the exterior surfaces Do not flood the display panel with excessive moisture Any moisture that seeps through could damage the internal electronics Q Repeat wipe down every 2 or 3 minutes for about 10 minutes total Remove the decontamination solution by thoroughly wiping surfaces with a cloth soaked in water Immerse or generously spray the carousel and lid with disinfection solution Allow to sit for 20 to 30 minutes OD Thoroughly rinse the carousel and lid with water bioM rieux strongly recommends distilled water PREVI Color Gram User s Manual Maintenance and cleaning Disinfecting and or sterilizing 10 min 5 20 30 min 5 21 Maintenance and cleaning Instrument shipment Instrument shipment WARNING Before the PREVI Color Gram is return
11. 4501 1689 en CE sl PREVI Color Gram USER S MANUAL BIOMEIRIEUX bioM rieux S A 69280 Marcy l Etoile France Tel 33 0 4 78 87 20 00 Fax 33 0 4 78 87 20 90 http www biomerieux com Printed in France 673 620 399 RCS Lyon O 2008 bioM rieux S A REF 29675 version B 03 2009 The performance characteristics and the results of the products presented are subject to a proper and skilled use of the product in strict conformance with the User s Manual Photo and illustration credit bioM rieux bioM rieux the blue logo and PREVI are used pending and or registered trademarks belonging to bioM rieux S A or one of its subsidiaries Revisions The list of revisions below summarizes replacements or additional pages in your User s Manual Date of printing Modifications Pages modified A 09 2008 Creation All e 03 2009 Addendum 4501 1842 insert Table of Contents 1 How to use this manual 1 1 Elle Te EE 1 1 SPS CII WAN INNING EE 1 1 General 2 1 FF UIC HOMANMCSS CHOON ssa de eeste cena leu due diet ete ee peser 0 2 1 RSS ence saeco ue see ant ade vue Suse ucaeae cen ceaeaeuant geese au aeembeot oe cue senecet sec ane ccan ener sucooaeaesanteceusecetcads 2 1 Ja ae et e 2 1 le o AA A a a 2 2 E A A E 2 2 TEE Seeerei 2 2 Run timing sequence and reagent consumption General references 2 3 A 2 3 DES CNI o od 2 4 Front pa
12. EVI Color Gram In all cases of maintenance and repair work should only be undertaken by a skilled technician Untrained personnel should not attempt to dismantle the instrument Only spare parts supplied or specified by bioM rieux should be used Fitting of non approved parts may affect the performance and safety features designed into the instrument If the equipment is used in a manner not specified in the instructions for use provided by bioM rieux the protection provided by the equipment may be impaired If in doubt contact your bioM rieux representative 1 2 PREVI Color Gram User s Manual Functional description Key features Intended use WARNING The PREVI Color Gram is designed to Gram stain specimen smears on microscope slides A Gram stain aids in the presumptive diagnosis of the illness and it gives preliminary classification of the causative agent This microprocessor controlled slide stainer is dual purpose Additionally the PREVI Color Cytocentrifuge Rotor allows to quickly sediment sample cells onto microscope slides for staining or other purposes The chemical mechanism of Gram differentiation is based on the differential cell wall permeability to the Crystal Violet lodine complex The Gram stain yields a specimen in which the Gram positive organisms are purple to black and the Gram negative organisms are pink to red PREVI Color Gram s features are e minimized reagent consumption
13. Manual Maintenance and cleaning Nozzle handling 5 Run the reagent into the tube of until the fluid runs free of bubbles The plunger can be withdrawn further to accept more fluid Do not pull the plunger completely out of the barrel of the tool Remove the nozzle adapter tubing and express the collected fluid into the stainer bowl Reinstall the nozzle Perform pattern and volume tests before reusing the instrument IMPORTANT If the test is still not correct contact your bioM rieux representative PREVI Color Gram User s Manual 5 9 Maintenance and cleaning Volume test Volume test The maintenance kit is required for this test that must be performed weekly Press n Hold a volume test tube small tube in place so that the nozzle C is covered to capture dispensed liquid Remove and close the tube Record the reagent letter on the tube 5 10 PREVI Color Gram User s Manual Maintenance and cleaning Volume test Place the tube in position C Q Repeat steps 3 4 5 and 6 for all the other nozzles Dr Dr A B and E Check each tube Tolerance according to the following table Minimum Maximum M If the volume is in the tolerance range go to point 9 If the volume obtained is out of the tolerance range e too low loosen the nozzle slightly e too high tighten the nozzle slightly And repeat the
14. ater then wipe dry with a clean cloth moistened with ethanol 70 Wipe with a lint free cloth Liquid spills If any liquid is spilled on the PREVI Color Gram it should be removed immediately to avoid affecting the performance of the equipment DANGER If liquid spilled on the PREVI Color Gram is potentially infectious it should be disinfected in accordance with any local applicable regulations CAUTION For disinfection solutions and procedure see Disinfecting and or sterilizing page 5 19 Note These decontamination procedures are for routine use only If you are shipping the stainer to bioM rieux for repair or service you must contact bioM rieux s representative for a current copy of the decontamination and shipping instructions before preparing and shipping the stainer Shipping the stainer without decontaminating it according to these instructions is dangerous to service personnel and will result in significant decontamination work Preventing injury If a slide breaks inside the stainer during a staining cycle stringent precautions must be taken during clean up especially if the instrument has been processing dangerous pathogens Always use protective gloves safety glasses and forceps when removing broken glass from inside the stainer f e Glass shards can be embedded in the walls of the stainer bowl These can cause serious cuts and pose a danger of infection Always remove these shards with a s
15. can be attracted to the magnets on the bottom of the rotor and cause damage if spun free of the magnets during spinning of the rotor IMPORTANT Never allow fluid to rise above the base of the drive hub Do not spray fluids near openings in the stainer housing that will allow fluid into the instrument interior This may cause severe damage to the instrument WARNING The disinfection procedure and the disinfectants should comply with the local applicable regulations Under normal clinical use the PREVI Color Gram poses very little risk of biological infection to laboratory workers Before the PREVI Color Gram is returned to bioM rieux all outer surfaces must be disinfected A disinfection declaration must be completed by the operating authority otherwise the PREVI Color Gram may not be accepted by the distributor or service center or customs authorities may hold it Disinfection solutions The outer surfaces of the PREVI Color Gram should be disinfected using a surface disinfection solution such as ethanol 70 mild detergent bleach solution for sporicidal disinfection use a 2 alkaline activated glutaraldehyde solution allow the solution to react for 10 hours PREVI Color Gram User s Manual 5 19 Maintenance and cleaning Disinfecting and or sterilizing Disinfection procedure Prepare a suitable container for all disposables Mask the lid latch and locking pin holes with waterproof tape
16. craper before attempting to remove loose glass Use a vacuum or adhesive tape to pick up loose glass inside the stainer bowl 5 18 PREVI Color Gram User s Manual Maintenance and cleaning Disinfecting and or sterilizing Disinfecting and or sterilizing All parts of the PREVI color Gram that came into contact with biological samples patient samples positive control samples or hazardous material must be treated as potentially infectious areas DANGER Reagents used with the PREVI ColorGram contain moderately hazardous chemicals that require care in handling Always use appropriate safety measures including gloves and eye protection when handling reagents f e The disinfection procedure should be performed in a well ventilated room by authorized trained personnel wearing appropriate individual protection equipment it is very important to thoroughly disinfect the PREVI Color Gram before removing it from the laboratory or before any technical service is performed on it This procedure may not be effective against prions Prior to cleaning disconnect the PREVI Color Gram from the main power supply to avoid any risk of fire and explosion CAUTION Do not flood the bowl by overloading the drain Never load chipped or cracked slides into the instrument Failure to use slides in good condition can lead to breakage during the cycle Make certain there are no small ferrous metal objects in the bowl These
17. cycle continues this indicates an electronic problem 1 Switch the stainer OFF 2 Wait 10 to 20 seconds 3 Switch power ON again to reset the instrument If the problem persists please contact your bioM rieux representative PREVI Color Gram User s Manual 6 3 Troubleshooting Error messages and Troubleshooting Table Description and Error Messages Recovery Procedure STAINING Abnormal staining on entire surface of all slides Abnormal staining on entire surface of some slides while other slides from the same carousel appear normal Streaks or bands of discoloration on one or more slides Check reagent levels in bottles Make sure the external reagent dip tubes are securely attached to each bottle Verify that each reagent pump is primed by opening the lid and pressing the manual prime button The nozzle should immediately spray a fine mist of reagent There should be no sputtering or hissing sounds which indicate the presence of air in the reagent lines Watch the external reagent pickup lines to see if there are any air bubbles present Air bubbles indicate inadequate priming or possibly an air or reagent leak in the system Air bubbles in any reagent line will likely cause poor staining Check nozzle performance using the Spray Pattern and Volume tests see Pattern and volume testing page 3 6 If necessary clean nozzle s If you are staining a full carousel 7 or more slides for
18. e may be an internal problem If the problem persists bioM rieux representative please contact your Troubleshooting Error messages and Troubleshooting Table Description and Error Recovery Procedure Messages If the display shows WRONG ROTOR a few seconds After you press RUN make sure the slide carousel is properly loaded on the drive hub In staining mode the instrument automatically detects whether the staining carousel is present before proceeding In cytocentrifuge mode the instrument will stop if it senses the staining carousel After you verify that the carousel is correctly loaded press RUN If the display still shows WRONG ROTOR there may be an internal problem Check carousel magnets If the problem persists please contact your bioM rieux representative The stainer does not begin a cycle when you press RUN PRIMING Press the priming button while listening carefully for the sound of the pump operating A reagent line will not prime even after following the procedures If you cannot hear the pump there may be an internal problem please contact your bioM rieux representative STAINING A small puddle of stain around the drain opening at the rear of the bowl or a few drops of stain around the bottom of the bowl is not unusual and will not cause a problem Stainer bowl fills with reagent after use If the bowl is filling with a large quantity of stain check the exter
19. ed to bioM rieux it must at least be disinfected A disinfection declaration must be completed by the operating authority see Disinfection declaration page 5 22 If a disinfection declaration is not supplied the PREVI Color Gram may not be accepted by the distributor or service center or customs authorities may hold it Ship your instrument in a container comparable to its original packaging Shipping conditions Temperature 0 to 40 C Humidity lt 80 non condensing Disinfection declaration The following disinfection declaration MUST be printed and completed by the operating authority and attached to the top of the package in which the PREVI Color Gram is returned before being sent to bioM rieux Disinfection declaration declare that the PREVI Color Gram in this package has been disinfected to remove or inactivate any biological material patient samples or hazardous material which could be dangerous to personnel or that it has never been exposed to any biohazardous material COCO ctr fash rte ced a ated ns Company institution e eege Funcion un T E A E R Phones Fak O 0 ao EME AA Dat Ordisintections NN Saude hd ae a Date name EE Signatures add ds 5 22 PREVI Color Gram User s Manual Maintenance and cleaning Fuse replacement Fuse replacement DANGER To prevent the risk of fire the main fuses should only be replaced with fuses of the same type and rating If fuse failure
20. entative PREVI Color Gram User s Manual Troubleshooting Error messages and Troubleshooting Table Description and Error Recovery Procedure Messages Specimens are washing off If you are heat fixing slides Make sure you are slides using adequate heat Try fixing some slides with alcohol in addition to heat to verify the fixation step Try to make your smears as thin as possible for a given specimen to minimize fixation problems Be sure to use clean premium quality slides If you are using the instrument s alcohol fixation function make sure the fixation function is activated the display reads FIX normal or fix HIGH If using normal fixation switch to high to apply more alcohol Check the alcohol Reagent E level in the bottle Check the alcohol Reagent E nozzle spray pattern and spray volume If the problem persists please contact your bioM rieux representative PREVI Color Gram User s Manual 6 5 e Sun l es LA Ou LA eg eg Ou e gt Su ah Fa _ EB ICE RRE E rc gent met um eege 6 E g 5 F E L li KZ GP Neal i mm CT d Es E Ga ABBA er ER 3 d SC ATHLNOW Yo Sir e CRD ATAIIM T A Yad me Aung EEI 2180 BALE AAN SOUFLE URU SD ELELU 3 20 LYVHO JINVNALNIVW JALLN3IA 34d 7 1 PREVI Color Gram User s Manual
21. es Outside dimensions Width 560 mm 22 inches Depth 530 mm 21 inches Height Lid open 580 mm 23 inches Weight 16 8 kg 37 lbs Ambient temperature Operation 15 C to 30 C 59 F to 95 F Storage 20 C to 60 C 4 F to 140 F Relative humidity maximum 80 at up to 31 C Altitude lt 2000 m Pollution degree 2 Over voltage category Il O e da PARAMETERS CHARACTERISTICS Readent pra VINOS les Each reagent has a separate spray nozzle g pray to dispense the correct amount of reagent A Fuchsin or Safranin with or without acetone B lodine Reagent channel C Crystal Violet D Distilled water E Ethanol or methanol Carousel capacit ie lege RS 30 slides Run 9 operator selected decolorizer cycles Program no 2 and no 3 validated by bioM rieux 20 rpm for staining rpm revolutions per minute Rotor speeds 950 rpm for drying Not provided by bioMerieux Since distilled water and ethanol or methanol are not available from bioM rieux it must be obtained locally Water should be filtered 0 2 micron inline filter to remove bacteria and particulates Ethanol or methanol should be 99 8 pure 2 2 PREVI Color Gram User s Manual General References Run timing sequence and reagent consumption General references The following table includes the general references for a staining cycle at DECOLORIZER 3 Slides carousel 12 30 Phases PUMP Time Volume Time Volume sec ml Sec ml 0 FIXATION E 70
22. f hazardousness and to treat and dispose of them or have them treated and disposed of in accordance with any applicable regulations YEARLY maintenance Contact your bioM rieux representative PREVI Color Gram User s Manual 5 3 Maintenance and cleaning Nozzle handling Nozzle handling Disassembly The maintenance kit and the cleaning solution are required IMPORTANT If the compression screw cannot be easily loosened use light penetrating oil and a 5 8 inch wrench to loosen the nozzle Perform the clean see CLEAN alcohol purge stage page 4 10 Remove the nozzle Disassemble the nozzle Remove the O ring and Z A discard it Gr d The O ring must be el Ce changed monthly Repeat previous actions 2 to 4 for each nozzle 5 4 PREVI Color Gram User s Manual Maintenance and cleaning Nozzle handling Cleaning Place the nozzle parts in the 50 ml centrifuge tube position Dr Note the reagent letter on the tube Fill with 25 ml of cleaning solution and cap the tube Shake the tube at least 10 times O Soak the parts as long as possible from 10 minutes to 12 hours Place the tube in its original location Repeat previous actions 1 to 5 for each nozzle PREVI Color Gram User s Manual 5 5 Maintenance and cleaning Nozzle handling Reassembly Empty the tube into the bowl with a skimmer or your thumb to
23. flushing procedure E Remove the remaining distilled water Load 200 ml of the diluted nozzle cleaning solution in the B line position and press Note pump approx 100 ml of the cleaning solution through the B line 6 Allow to sit for at least 1 hour up to 12 hours E 1202 h if possible Note in the meantime centrifuge mode can be activated by pressing After soaking load 500 ml of distilled water in B line position to rinse 5 14 PREVI Color Gram User s Manual Maintenance and cleaning B line flushing procedure and press Note pump approx 400 ml of distilled water through the B line 1 Load 200 ml of lodine and press 10 Reinstall the nozzle B Press PREVI Color Gram User s Manual 5 15 Maintenance and cleaning Priming all lines simultaneously Priming all lines simultaneously Q e to prime all the reagent lines The pumps will run for 60 seconds Long term storage If the PREVI Color Gram is inactive for more than a week perform the following steps This long term storage procedure will prevent nozzle plugging from occurring when the instrument is ready to be used again Unscrew the cap and remove the dip tube from the empty bottle Place the dip tube in a bottle of ethanol or methanol 99 8 pure 5 16 PREVI Color Gram User s Manual Install a cap on the reagent bottle
24. ght is ON Place the empty carousel in the instrument and close the lid Press Open the lid and Switch OFF the instrument Spray and wipe the interior of the bowl and the nozzles with ethanol 70 IMPORTANT remove the carousel The CLEAN cycle must be used each time the PREVI Color Gram is left on stand by FOR MORE THAN 3 HOURS The alcohol purge stage must be performed after each staining period and the prime stage must be performed just before restarting the instrument This stage requires the use of 10 ml of alcohol divided equally between each nozzle After this first cleaning stage if the instrument remains on stand by for more than one week please refer to the Long term storage page 5 16 PREVI Color Gram User s Manual Operating the PREVI Color Gram Alcohol fixation ethanol or methanol Alcohol fixation ethanol or methanol Automated fixation can be used in place of manual fixation WARNING bioM rieux strongly recommends the use of the HIGH fixation mode and not the NORMAL mode ALCOHOL fixation in normal mode is shorter and may lead to specimen loss The ALCOHOL FIXATION is OFF by default Que Or See Starting a staining cycle page 4 8 Note The ALCOHOL fixation can be deactivated by pressing 2 in step 3 of the above procedure This setting is kept in memory PREVI Color Gram User s Manual 4 11 Operating the PREVI Color
25. he Volume test page 5 10 End of the week Wipe the carousel tray and lid using paper towel with ethanol 70 Flush the waste tubing with 200 to 300 ml of ethanol 70 to prevent clogging Empty the waste container see Emptying the waste container page 4 13 Check that the maintenance procedures have been completed by following the Preventive maintenance chart page 7 1 and then sign with your initials PREVI Color Gram User s Manual MONTHLY maintenance MONTHLY Eu e 063 4 E l 1833 d Po a Maintenance and cleaning Preventive maintenance plan Disassemble and clean all the nozzles see Disassembly page 5 4 Perform the B flushing procedure see B line flushing procedure page 5 13 Perform the Pattern test see Pattern test page 4 3 Perform the Volume test page 5 10 If tests are incorrect see Nozzle handling page 5 4 and repeat the tests with the cleaned nozzles Check that the maintenance procedures have been completed by following the Preventive maintenance chart page 7 1 and then sign with your initials WARNING WASTE DISPOSAL Dispose of used or unused reagents as well as any other contaminated disposable materials following procedures for infectious or potentially infectious products It is the responsibility of each laboratory to handle waste and effluents produced according to their nature and degree o
26. il the CLEAN cycle is completed Pattern test Or pea PREVI Color Gram User s Manual 4 3 Operating the PREVI Color Gram Pattern test Hold a sheet of white paper in front of nozzle A Check the quality of the pattern If the pattern is not correct see Nozzle handling page 5 4 Repeat steps 3 4 and 5 for all the other nozzles Dr Dr B C and E Press ES 4 4 PREVI Color Gram User s Manual Operating the PREVI Color Gram Loading slides Loading slides CAUTION Never load chipped or cracked slides into the instrument Failure to use slides in good condition can lead to breakage during the staining cycle If slides do break in the bowl see Cleaning page 5 18 Make certain there are no small ferrous metal objects on the lab bench These can be attracted to the magnets on the bottom of the carousel and cause damage if spun free of the magnets during spinning of the carousel Slides must be loaded in balanced pairs If an odd number of slides need to be stained use a blank slide IMPORTANT Carousels must be loaded with similar specimens for a similar level of staining Otherwise bioM rieux does not guarantee the staining performance Remove the carousel from the bowl D gt E y ee e ls o T mn z A gt Remove the carousel lid by pressing the button and lifting the lid away PREVI Col
27. ing Table Theme Description and Error Recovery Procedure Messages There is no power to the stainer Check the electrical outlet and the power cord when the power switch is turned Connection ON Check the fuses by disconnecting the power cord from the power entry module then opening the fuse door For continued protection replace only with fuses of the type and rating indicated on the rear panel of the stainer CAUTION Fuse failure may indicate a serious internal problem DISPLAY Strange information shown on 1 Switch the stainer OFF the display and or erratic stainer operation The stainer does not begin a cycle when you press RUN The display is blank when the POWER light is lit If the display shows PREVI Color Gram User s Manual 2 Wait 10 to 20 seconds 3 Switch power ON again If this works temporarily but the problem recurs install a computer type surge suppressor to protect the instrument from power line transients If possible connect the stainer to a power circuit that is not shared by centrifuges refrigerators air conditioners or other motorized equipment 1 Switch the stainer OFF 2 Wait 10 to 20 seconds 3 Switch power ON again If the display is still blank there may be an internal problem If the problem persists bioM rieux representative please contact your Verify that the lid is fully closed and the latch is engaged If the Lid Not Shut indication remains ther
28. into an appropriate biohazard bin in accordance with local regulations Connect the waste tubing to the empty waste container The instrument is ready Note bioM rieux recommends emptying the waste container once a for use week see Maintenance and cleaning page 5 7 Other settings WARNING The information contained in this section is intended for customers who wish to use this equipment with applications other than those specified by bioM rieux In that case the user assumes complete responsibility for that use Decolorizer setting The decolorizer cycle intensity can be changed according to the type of specimens or the staining results expected IMPORTANT Levels 2 and 3 have been validated by bioM rieux level 3 default setting Keys DECOLORIZER CYCLE ULTRA THIN SPECIMEN LEVELS RECOMMENDED BY BIOMERIEUX BIOM IRIEU Xx we CO ULTRA THICK SPECIMEN PREVI Color Gram User s Manual 4 13 Operating the PREVI Color Gram Other settings Press Enter the required DECOLORIZER level Example for a very thick specimen press See Starting a staining cycle page 4 8 4 14 PREVI Color Gram User s Manual Operating the PREVI Color Gram Other settings Stain adjustment These settings can be used if the desired staining is not achieved through the decolorizer settings alone Once you have found the decolorized setting that works you can adjust the amount
29. ionized water It contains at least 1 of Oxalic Acid Dihydrate the Material Safety Data sheet is available on request The nozzle cleaning solution must be reconstituted before use by adding 2 5 liters of ethanol C2H50 or methanol CH40 99 8 to the 5 L bottle This instruction is symbolized on the bottle label by the following mention gt 2 5 L C2H6O or CH40 The reagents required but not provided ethanol or methanol may present a chemical risk e and should be handled with care Reconstituted Nozzle Cleaning Solution with ethanol or aay methanol can be hazardous and should be handled with the same care N un EA NOZZLE CLEANING SOLUTION VV mm K mm The concentrated nozzle cleaning solution must be stored at 15 25 C until the expiry date indicated on the bottle label After reconstitution the solution must be stored at 15 25 C until the expiry date For practical reasons the solution can be transferred into a 500 mL or a 1 liter bottle In this case it is important to record the expiry date on the bottle Please refer to Maintenance and cleaning section page 5 4 Waste disposal the Nozzle Cleaning Solution effluents must be treated in the same manner as the reagents see warning page 5 3 Note The nozzle cleaning solution is available separately Please contact your bioM rieux representative Other parts Since the following parts are not available from bioM rieux they m
30. n the waste container is full empty it see Emptying the waste container page 4 13 WARNING bioM rieux recommends the use of the default DECOLORIZER setting IMPORTANT Use E as an emergency stop when required e g abnormal vibration or noise the staining cycle will be aborted ALCOHOL FIXATION is OFF by default If it needs to be ON see Alcohol fixation ethanol or methanol page 4 11 If the carousel is full press Indicates the number of slides e g 6 If not enter the number of slides that have been loaded into the carousel even number Then press Indicates the progression of the program running for the named reagent CAUTION E d A signal tone sounds at the end of the cycle Se 4 8 PREVI Color Gram User s Manual Operating the PREVI Color Gram Unloading slides Unloading slides WARNING Treat slides in accordance with good laboratory practice guidelines and local regulations Remove the carousel from the bowl Remove the carousel lid by pressing the button and lifting the lid away Carefully remove each slide already dried and read the Gram results with a microscope Negative Gram tousse Positive Gram O Discard slides into a biohazard container PREVI Color Gram User s Manual 4 9 Operating the PREVI Color Gram CLEAN alcohol purge stage CLEAN alcohol purge stage CAUTION e ODS Be sure the stainer li
31. nal drain tube Make sure the drain tube is properly connected to the drain port on the stainers rear panel The tubing must run continuously down toward your lab drain or waste container with no loops rises or obstructions Make sure the end of the tubing is not submerged in liquid This can prevent proper drainage If you are using a waste container make sure the container is vented to allow air to be displaced by the spent stain Check the drain tube for blockage or foreign material If the problem persists please contact your bioM rieux representative Check the stainer bowl to make sure there is no foreign material interfering with the rotation of the drive hub and carousel If the display shows Turn the hub or carousel by hand it should turn freely 1 Switch the stainer OFF 2 Wait 10 to 20 seconds The stainer does not begin a cycle when you press RUN 3 Switch power ON again and try again Continued DRIVE PROBLEM display indicates an internal problem please contact your bioM rieux representative 6 2 PREVI Color Gram User s Manual Troubleshooting Error messages and Troubleshooting Table Description and Error Recovery Procedure Messages Make certain the rotor is balanced and that the If the display shows carousel is seated correctly on the hub Verify keyboard operation by observing the display while entering slide number settings The settings should appear on the display as
32. nel and keypad description BE 2 4 BACK DANG lie 2 5 SEN 2 5 Mamtenance KI es rate ne dant na ta boat are ane aa attests a nie 2 5 NOZZE E E ia 2 6 NOZZIC ClEAMING Ree EE 2 6 EIERE 2 6 Installing the PREV Color Gram 3 1 Blatt Iesel tele el 3 1 Installation ee ee EE 3 2 Jee Vie e WASTS UDI Osdia AE 3 2 CGMMECUAG DOW EE 3 3 installing E Le E elen 3 4 Pr paration PrOCOdUrO ES Sn E a D is 3 5 Verde ln e EE 3 5 ee RN el in TS SUNG EE 3 6 Operating the PREVI Color Gram 4 1 CUBAN Tepime Sta ase a i ee o 4 2 Pantene is tere erro Re O A Oper eran gener a ore a 4 3 LOGIN SIND E 4 5 Staningd a staining Bee EE 4 8 Bn Metall Ee 4 9 CLEAN alCOMOl DUNG Sta ER 4 10 Alcohol fixation ethanol or methanol seen 4 11 WMONMONNG re agentievel EE 4 12 Replacing a reagent boleta EE 4 12 Emptying the waste De EEN 4 13 OINGrSCUINGS arisia R EE eee Lt 4 13 A 4 13 P a 4 15 PREVI Color Gram User s Manual Table of Contents 5 Maintenance and cleaning 5 1 Preventive maintenance Pla EE 5 1 Preventive Maintenance CAF dee EE 5 1 SIB ge Mer Ein Le EE 5 2 WEEKLY gn Ela ne 5 2 MONTHLY mantenan aussi elias 5 3 YEARLY maintenance ccccccccececeeeceeeceueeceeeceueceueeceessueceeeseeeceueeseesaeessueeeueesaeeseessueeseeesseueesaeesseessuseneeess 5 3 NO ZI IVA CM Ol WEE 5 4 DISASS MONA A rot io ner tan nine 5 4 EE Un le e EE 5 5 KI EE 5 6 Unclio gg ING AMG TUBING EE 5 8 A 5 10 BING MUSING Beleg er 5 13 Bidon ele URL Eat e Ee
33. of Crystal Violet C and lodine B according to 3 settings Low Default and High Recommended setting is Medium A setting of reagent C High and reagent B High will produce darker Gram positive organisms A setting of reagent C Low and reagent B Low will produce lighter Gram positive organisms O Or for the Crystal Violet for a high level See Starting a staining cycle page 4 8 Note For setting the IODINE level simply press a in step 3 instead of 2 PREVI Color Gram User s Manual 4 15 9 Maintenance and cleaning A DANGER Reagents used in the PREVI Color Gram contain moderately hazardous chemicals that require care in handling Always use appropriate safety measures including gloves and eye protection when handling reagents f e CAUTION Before performing any maintenance operations remove all the slides from the carousel bioM rieux strongly recommends following this maintenance plan to keep the PREVI Color Gram in good operating condition One week is the maximum time between two successive staining runs If the PREVI Color Gram is not used for more than one week the long term storage procedure must be applied see Long term storage page 5 16 Preventive maintenance plan The preventive maintenance chart is a support to be used e DAILY e WEEKLY e MONTHLY e YEARLY and each time it is necessary to intervene on the instrument and i
34. opriate safety measures including gloves and eye protection when handling reagents e Right side panel with 5 prime buttons for 5 reagent channels Fill the E bottle with ethanol or methanol 99 8 pure and the O future use e g long term storage D bottle with distilled Fuchsin or water D Safranin with or without 5 D acetone p lodine Place each bottle in Crystal Violet the appropriate CT position En Distilled water N Ethanol or w s methanol Open the new bottle record the letter on the ze cap and keep it for Ee Insert the dip tube E into the reagent bottle E and screw on the ring cap Repeat step 3 for bottles D C B A Note For frequent use 5 L bottles D amp E are available see References page 2 3 To install the bottles cut the tubing just above the dip tube Install the bottles in a drip tray 3 4 PREVI Color Gram User s Manual Installing the PREVI Color Gram Preparation procedure Preparation procedure IMPORTANT To obtain optimum performance thoroughly purge and prime each reagent delivery line using the following instructions CAUTION This procedure must be applied before the 1 use of the instrument WARNING Never operate a dry pump for more than 10 seconds Nozzle checking Remove each spray nozzle with the nozzle tool by turning the counter clockwise Note the location of each nozzle so you can return it to the o
35. or Gram User s Manual 4 5 Operating the PREVI Color Gram Loading slides TOP VIEW CAROUSEL Insert the slides into Specimen side the carousel N The slides must be inserted diametrically opposite one to the other to balance the carousel O Be sure that slides are loaded in balanced pairs If an odd number of slides need to be stained use a blank slide CAUTION A signal tone sounds during the run if the position of the slides is 7 unbalanced 4 6 PREVI Color Gram User s Manual Replace the lid by pressing the button and lowering the lid over the indexing posts Release the button and press the lid handle until it clicks into place Q Place the carousel in the bowl and close the lid PREVI Color Gram User s Manual Operating the PREVI Color Gram Loading slides Operating the PREVI Color Gram Starting a staining cycle Starting a staining cycle CAUTION A new cleaning cycle should be performed if the time between runs exceeds 3 hours The reagent and waste liquid level must be monitored by the user To ensure proper staining and to protect the stainer never allow a reagent line to run dry When the reagent liquid level is near the bottom of a reagent bottle replace the bottle see Replacing a reagent bottle page 4 12 DO NOT FILL ABOVE THIS LINE never allow the waste liquid level to go above the maximum safety level Whe
36. possibility of condensation causing a hazardous situation Keep the waste container tube straight and as short as possible 2 meters 79 inches maximum The waste container tube should be positioned at a lower level than the table If possible bioM rieux recommends you to install the instrument at an extremity of the bench top Installing waste tubing Clip the waste tubing into the PREV Color Gram and push until you hear a click i E d Cat Come AN 30 SSD Adjust the tube length L lt 2m Connect the waste tubing to the waste container Drip tray Install the waste container into a drip tray to prevent from spilling any liquid 3 2 PREVI Color Gram User s Manual Installing the PREVI Color Gram Installation procedure Connecting power Make certain the switch is OFF O Check that the voltage label complies with the power supply Insert the electric cord into the power socket O Plug the power cord into the mains AC power Turn the power switch ON 1 D After 2 seconds the digital display will show X firmware version PREVI Color Gram User s Manual 3 3 Installing the PREVI Color Gram Installation procedure Installing reagent bottles A DANGER Reagents used in the PREVI Color Gram contain moderately hazardous chemicals that require care in handling Always use appr
37. riginal location Hold a sheet of white paper in front of the nozzle A Press and hold each prime button Stain should appear within 5 seconds If this is the case go to step 6 If not immediately stop the procedure and see Unclogging the tubing page 5 8 PREVI Color Gram User s Manual 3 5 Installing the PREVI Color Gram Preparation procedure Flush with distilled water and wipe dry the bowl and the nozzles Q Replace each spray nozzle by hand and then with the nozzle tool by turning clockwise The Pattern test page 4 3 Then the Volume test page 5 10 IMPORTANT After this preparation procedure follow the ROUTINE use described in Operating the PREVI Color Gram page 4 1 3 6 PREVI Color Gram User s Manual 4 Operating the PREVI Color Gram SETTING THE NUMBER OF SLIDES ALCOHOL FIXATION PREVI Color Gram routine process Key Orange line run process Blue line maintenance process Grey elements optional part This routine process flow diagram describes the necessary stages for the routine use of the instrument Details are given in the following sections PREVI Color Gram User s Manual 4 1 Operating the PREVI Color Gram CLEAN reprime stage CAUTION The reagent AND waste liquid levels must be monitored by the user To ensure proper staining and to protect the instrument never allow a reagen
38. rs Cytocentrifuge mode selection Run a staining process I Access to PATTERN and VOLUME tests see Pattern and volume testing page 3 6 Program the staining setting Power Rotation 3 3 3 Cleaning process Abort any operation or Exit o sexy EE E y y y zen y 4 Color Gram EE e Numeric keys programs Control keys 2 4 PREVI Color Gram User s Manual General Description Back panel Volume button signal tone setting Exhaust vent Power switch On Off 3 O Fuse door alternative current see Specifications page 2 2 Waste tubing Power cord Spray nozzle orifices DR Distilled water Rear E Ethanol or methanol C Crystal Violet B lodine A Fuchsin or Safranin with without acetone Dr Distilled water Front Drive hub Maintenance kit 6 nozzle tubes 6 volume test tubes 1 nozzle tool 1 cleaning wire 1 unclogging tool 1 nozzle disassembly tool 2 nozzles 4 nozzle O ring Grease silicon lubricant PREVI Color Gram User s Manual 2 5 General Description Nozzle Compression screw Q Nozzle housing Swirl cone O ring Nozzle cleaning solution The nozzle cleaning solution is used for the PREVI Color Gram maintenance operations The 2 5 L concentrated nozzle cleaning solution reference 29525 is contained in a 5 liter bottle The concentrate is composed of 25 2 g of Oxalic Acid Dihydrate per liter of de
39. t line to run dry When the reagent liquid level is near the bottom of a reagent bottle replace the bottle see Replacing a reagent bottle page 4 12 the waste liquid level to be above the maximum safety level When the waste container is full empty it See Emptying the waste container page 4 13 DO NOT FILL ABOVE THIS LINE IMPORTANT To obtain optimum performance thoroughly purge and prime each reagent delivery line using the following instructions WARNING Never operate a dry pump for more than 10 seconds gc The CLEAN cycle is a two stage process that re primes reagents into the nozzles then uses ethanol or methanol 99 8 pure to purge the reagent nozzles of precipitates and debris CLEAN reprime stage IMPORTANT If you have not used the PREVI Color Gram before go directly to Installing reagent bottles page 3 4 This stage must be completed before restarting several runs to complete the CLEAN cycle ge The CLEAN reprime stage requires the use of 7 5 ml of A B C reagents Alcohol lodine Crystal Violet and 10 ml of reagent D Distilled Water divided equally between each nozzle Switch on the instrument Open the lid and insert an empty carousel matching the proper position Close the lid 4 2 PREVI Color Gram User s Manual Operating the PREVI Color Gram Pattern test Perform a PATTERN test Note If a CLEAN cycle is aborted a warning message will be displayed unt
40. tion light on the front panel and listen for the sound of carousel rotation to cease The anhydrous alcohol used in the instrument is highly flammable A break or malfunction in the reagent delivery system has the potential of releasing up to 500 ml of alcohol in and around the instrument If this occurs carefully shut off the power to the stainer and consult the safety sheet for information in handling alcohol spills Do not use the instrument again until any leaks are repaired Regarding the PREVI Color Cytocentrifuge Rotor lid and rotor gaskets and related components are intended to be part of biosafety systems such as are specified in international and national biosafety guidelines and cannot be relied on as the only means of safeguarding workers and the environment when handling pathogenic micro organisms bioM rieux recommends distilled water for reagent D and ethanol for reagent E Methanol can also be used for reagent E nevertheless its use is subject to specific safety precautions and requires the instrument to be placed under a safety hood To avoid serious instrument damage never use reagents other than those supplied by bioM rieux Except for both reagent mentioned above using reagents not supplied by bioM rieux may seriously damage the stainer and may void your warranty PREVI Color Gram User s Manual 1 1 How to use this manual Specific warnings IMPORTANT Please read this manual carefully before using the PR
41. ts parts Preventive maintenance chart Cleaning cycle reprime stage followed by pattern test Cleaning cycle alcohol purge NS Signing with initials NS Wiping the bowl DS Volume testing d k WEEKLY E23 4 5 6 amp Complete with your inifals Kr Wiping the carousel E Pattern test Waste tubing flushing Nozzle maintenance B line flushing PREVI Color Gram User s Manual 5 1 Maintenance and cleaning Preventive maintenance plan DAILY maintenance IMPORTANT CAUTION DAILY WEEKLY maintenance Check reagent levels and expiry dates This procedure is applicable when the time between two runs exceeds 3 hours After the last run Before restarting a new staining run successively perform the CLEAN cycle see CLEAN reprime stage page 4 2 the PATTERN test see Pattern test page 4 3 the CLEAN cycle alcohol purge stage after the last run see CLEAN alcohol purge stage page 4 10 The instrument is on STAND BY and can be switched off ae Spray and wipe the interior of the bowl and the nozzles with ethanol 70 Clean the instrument lid with ethanol 70 Check that the maintenance procedures have been completed by following the Preventive maintenance chart page 7 1 and then sign with your initials WEEKLY KM2 3 4 5 6 KA 9 2 Beginning of the week Perform t
42. ust be obtained locally e ethanol or methanol more than 99 8 pure e distilled water Empty bottles can be provided by bioM rieux see References page 2 3 WARNING ETHANOL OR METHANOL are not provided by bioM rieux The customer should purchase them locally and observe the recommendations in terms of safety and chemical risk 2 6 PREVI Color Gram User s Manual 3 Installing the PREVI Color Gram CAUTION If you observe any damage on the packaging or equipment please contact your bioM rieux representative before installing the instrument Place the instrument with at least 30 cm 12 inches clearance from obstructions or hazardous materials Unpacking procedure Open the 2 boxes and remove the protection foam packaging Check the contents according to the packing lists included in the 2 boxes instrument and accessories ge o compose Ez LE PES Lift the stainer out of the box Place the instrument on a flat surface free from dust and Vibration and away from direct sunlight PREVI Color Gram User s Manual 3 1 Installing the PREVI Color Gram Installation procedure Installation procedure CAUTION Ensure that the distance between the back panel of the PREVI Color Gram and the wall is at least 30 cm 12 inches Before the PREVI Color Gram is installed and switched ON it should be left to stand for at least three hours so that there is no
43. you press the intensity keys 1 Switch the stainer OFF 2 Wait 10 to 20 seconds The stainer does not begin a cycle when you press RUN 3 Switch power ON again and try again If the problem persists please contact your bioM rieux representative STAINING Stain is leaking onto the lab Check all external reagent lines for visible signs of bench cracks or loose fittings Make sure the drain outlet inside the stainer bowl is not blocked by any foreign material Make sure the drain tube is securely attached to the drain port and that the tubing is not cracked or deformed If the problem persists please contact your bioM rieux representative STAINING The stainer fails to spray To allow programmed staining of partial loads the reagent during a staining cycle stainer monitors the position of the carousel as it rotates in the bowl Stain is sprayed only in the correct position This causes the actual cycle time to and or vary depending on the position of the carousel at the beginning of the cycle continues to run after the cycle This is normal stainer operation However if the should be complete cycle continues for an abnormally long period or if the bar graph and percentage complete icon do not change after 1 minute it may indicate an electronic problem or an internal problem To determine this press the STOP button If the cycle stops this indicates a problem with the carousel position sensor If the
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