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EMCS CW/RW Requirements Integration Report N-USOC-REQ

1. Filtered Zeolite below 0 5mm diamter 4 Pour the filtered Zeolite above 0 5mm diameter into a clean container or glass See picture below y m Doc no EMEX PR MULT 04 PRO prototech Z EMCS Experiments Int Doc no PR_30193_PR_05 Procedure for Filtering of Zeolite issue B Date 03 05 2007 Project 30193 Description Page 12 of 19 5 The Zeolite is now finished filtered and ready for use Filling of growth pot shall be done according to Growth Pot Filling Procedure from Multigen 1 PI Zeolite ready for use above 0 5mm diameter 6T Jo abed 2002 S0 E0 eg g enss SO ud EGTOE Ud 0u 20G lul Oud v0 LINW ud XINI ou 3oq Aped Joujo Aue Ag Ped Ul JO IDUA ur pasopsip aq Jou EYS 3I paliddns SEM 3j U SV HDSLOLOYd JO uoissiuad uam SLI NOYEM DIM zo asod4nd 343 Joj Ajajos 3urajdra1 343 Aq pasn ag 01 si pue SY HJYLOLOUd 03 Asejajidoud si uj a rau paujeuo2 uoneuuojut SUL peusiuy Buli 3li J S dans UNOJISd A pH deis Wiojlag mw s e days woad x days woad L dos woad Ce ES Z S Je3deub o3 53e Busy Weis Busy J23deup ut gs 3ueuudinb3 3no mu pue te Jajdeyo 0 22e Juswd nba p y Jaydey gt ui uuosi d 3no 114 suonesedaid
2. Verification Zeolite rinsed Procedure Prototech Annex 1 Appendix 2 of properly to avoid particles 0 5 mm HMST set up in June July 07 Astrium QA Annex 4 Verification 2 Form Table 1 Documentation of work performed at N USOC as part of the CW RW flight H W preparation Multigen 1 PCC assembly and seed set up Procedure Verification material Certificates CW RW Pls Annex 2 4 Seed Certificates loaded exactly as listed in for Arabidopsis Seeds WT GM GUS lefty and hmg Annex 3 Phytosanitary Certificates for Arabidopsis Seeds WT GM GUS lefty Report N USOC N USOC TR 027 Cell Wall Resist Wall PCC Assembly and Seed Set Up Report ADO4 Report IOT EMCS RP 4000 033 DOR CWRW EC Checkout and Inspection Report Final Flight Preparations AD05 Correct Growth support and seed installation and assembly of CW RW PCCs Final verification of ECs by IOT CWIRW PCCs Provided by Documentation Preparation at N USOC Type attached filled in for Multigen 1 flight HMST and hmg Plant seed import permit NASA Annex 5 Seed Import Permit forma invoice Shipping packing list NASA Cover letter Pro Annex 6 NASA Cover Letter Pro forma invoice and Shipping packing list N USOC PRO 005 Tm mW CW RW Flight Sample Hardware Packing Transport issue 2 LE and Check Out Procedure Date 27 02 2008 Warweqtaw Bier Ennperi and Rseratians Fein Page 8 of 58
3. Figure 5 6 Figure 5 7 The flat sealing was not removed and after inspection PEEK and bottom plate were mounted again 5 2 3 PCC The EMCS EC with integrated PCC has been returned with 1J On 28 7 2008 EC FM092 was opened in the presence of Astrium PA W Biemann ESA S Hinderer M Zell JAXA Prof Yoda san In this PCC germination took place and the seedlings were removed at KSC by JAXA representative The PCC was disassembled acc to N USOC PRO 002 issue 1 1 removal of locking cover 2 removal of growth pot with PCC lid Fig 1 2 3 EU Dok Nr No EMCS RP 0000 151 DOR Ma Trouble Shooting for EADS j Ausgabe lssue 1 Datum Date 18 8 2008 SPR 283 ai Seite Page 15 von of 24 Fig 1 i Fig 2 The o rings at lid and water inlet hole are properly installed and undamaged Inside the growth pot dark black particles biological origin were found especially on the borosilicate layer Fig 5 6 the sponge Fig 12 the PCC felt Fig 9 the felt at air inlet mainly towards growth pot Fig 3 4 the zeolithe Fig 10 Fig 3 Fig 4 air inlet felt left 3 removal of air inlet felt Fig 4 Dark black colour visible mainly towards inside of PCC Fig 3 4 4 removal of PCC lid Fig 5 6 The borosilicate layer shows dark black particles No further inspection whether also seeds are visible Donuts are properly installed and
4. Part HMST Recon s Arabidopsis thaliana He Haz Lev z E Last modified 1 2208 123 014 Columbia wa N A 104 seul l ossible eye andre S Contact Motoslti d from the 4 fespiratory v7 Latin Kamada JAXA Subsystem Arabica hali min 4 fram finc particles of kamada motoshi a jaxa j Arabidopsis thaliana to 19 seeds jaxajp Plant Culnvation Columbia 4efiy mutant NIA per PCC dry Seite 181 29 868 3698 Amy Chamber PCC S PECs ton SSL Ref memo Maas amy a boer com Label Arahidopsts thaliana SFZEUTM Columbia mas mutant NA This Ween will be Arabidopsis thaltata pe Modal Columbia Transgenic promotcr GUS NIA Ern a lene produced during germination trace trace Cultivation Chambers arhan dioxide produced during germin hon trace trace SC Micro memo Zeolite Zeopronivs 10095 240 mm SE22 07 014 10 4 LO mm panicles 30 mm PCC x PEC Plant growth supplement u NEO Murashige art Skoog Mixture 0 1 PCC xs POU Pelysinsl alcohol PVA Un OK i n 01ml DC x S PCC ole January 25 2008 9 31 42 AM Mission Toxicoloyist S Langford sou 15 unraisy 9024 SEO byt O St bb xta Zoo abeg Aspon Cv 4I SZ 10 DO 3v 7124017 N USOC PRO 005 N USOG E CW RW Flight Sample Hardware Packing Transport issue 2 4 and Check Out Procedure Date 27 02 2008 Page 47 of 58 Annex 5 Seed Import Permit N USOE N USOC PRO 005 m CW RW Flight Sample Hardware Packing Transport
5. 3 Sugur buen Pures ME patton JON Sou maye 18 sed vim m 4 mr vagina sok 2m Beam sen acd male vous T E S du xes saa Y Par cam VIZA AVWTE Sanatuvadxa yo absan Aa J Ud BYLONT man NSS COQO OwWd 20 8n hN Frew a DM ue PYLE unomi moy an eA SHIBWOY 82UBJ9 O EUILUON uonduoseg dojs js91 on days 359 enpeooJd au 0j SUONBLBA pue SabueyJ G e qe I G e qe ut PSJUSLUNDOP aq EYS enpeooJg eui 0 suoneueBA pue seBueuo iv ul UODEUSA ainpadouy 1591 8 96 JO pz bed 400 S0 Ez ap CL anss eunpao204Jg dN I9S paag pue Ajquiessy Jod L Labninw Ay heuig p u voriesuiaj ZO0 OUd DJOSN N ah soueunssy PJEND uone2o ejeq q peuops och hall Sud SO 10 XT 7 BOI Tan 30000 m Weed VAM RAD DTH mw Hy uses cw Guo Swa Ws vence vo un O3 wyapd TH A yeon D mme egg PUD do usss u vows c acl Pardo vm PUD Spann to patou carn Jenso D2 my anne Ems usb a UN Guothdee a y fe pwent prea buu o mat a zenzaub euatOddo yb bp YNDET Qs vv kat 324 mal P det Ap yim ma gt o ven Mae mast veont anmanv bou o we Mum 22d Lat LAG yrmend vam Ad aa 40d yuma mat qo is TY V9 92 ovv an xy ad YO sab Guraab Piano oi Lossaooicmw o IT dd Ne bugurmua dur may ay 20d Pwo PUb mad ROUA LABA 2 Nyt HO MRU at iTA fan po dus aide Ee das poc O ad PIJIN con wmd pape sowfms PA 22d oH pm PAN Him ua o yeaov 429 mo m Wars 39 bunns Jar un Nyt vi AN Mam A wol aarm pn anman ddy 2220 WA jene oma
6. 4 Scope This document contains the specialized plan and dedicated fill in procedures for verifying 1 Correct packing at N USOC into dedicated transport boxes 2 Handling during transport from N USOC to KSC 3 Correct check out before handover at SSL Space Science Laboratory at KSC N USOC PRO 005 N US00 mm CW RW Flight Sample Hardware Packing Transport Issue 2 plis gt Lu Bars Leppert and erann Gesi and Check Out Procedure Date 27 02 2008 Page 9 of 58 5 Procedure detailed plan 5 1 Personnel The personnel required for this Procedure and their associated responsibilities are listed in Table 2 Engineer The engineer is responsible for e operation of measuring instrumentation data acquisition data handling data recording e identification and compilation of data sheet e personnel to be present in the set up area e the accuracy of the measured values e data report Manager The manager is responsible for articles and technical aspects of the set up performance examination of disturbances article integration and handling set up performance providing of reports Quality Assurance The Quality Assurance representative is responsible for e surveillance of equipment according to regulations as well as the procedure application e statement that the set up articles to be used have passed all checks e checking the identification markings on the articles e supervision of proceeding with respect to qu
7. ISSUE STATUS The status list shows which chapters are included which changes are introduced valid issue and issued date Ch 6 App A and Editorial updates based on comments B from ESA The information contained herein Is proprietary to PROTOTECH AS and is to be used by the recipient solely for the purpose of which it was supplied It shall not be disclosed in whole or In part by any other party without the written permission of PROTOTECH AS EMCS 5 Doc no EMEX PR MULT 04 PRO protote C h e Experiments Int Doc no PR_30193_PR_05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 CONTENTS Page 4 of 19 CONTENTS SCOPE EE sssupupuwanan E REFERENCE DOCUMENTS A PR PARTICIPANTS REQUIRED 7 EQUIPMENT LIST DESCRIPTION occcccesonenseennuvcossecncncscenesscemesceuseauecunaeneneenarenensnsscenspensssesenersanos D Equipment sees een gege Sue ENTE iD Filtering of Zeolite enne ARTIG NS STEP BY STEP PROCEDURE LELETET EE SRPERERERERREBESEMEAM FILE TETT TT EE TELE LE LEG 13 N ACCEPT REJECT CRTTERTA sssssssssssnasassnaanansasaasnassssasasassassssssa LO DEVIATION soennensnnnvanensennavnunnnvnnerenvnnsenunnenennnnnnnannanenunsunennnunnensnnnennneenenene
8. 1 p E 2275 3792 10425 f f 123309 amp t2 134 224 0 134 po x opo p o op C o C o LLL LL A3 FreshtoHU of oo o op 9 ffreshteEC 3073 2049 1829 05 15884 20 4533333 FreshtoWaste 0 o o 0o 0o 0 0 0 Don al Da 124 0 op jJ Debt 3 0 on 0o 0 A J 3 DH to Waste 23480 3913 11059 af 12421 Hutba 815 G zsa 1 63 po _ qu ipo qo p A4 FreshtoHU O oo 0o 0o 0 0 FreshtoEC 4997 3331 2927 s8o0266667 2070 276 FreshtoWaste_ of oo og op op 0 Dan f 64 12 eT pHiEC 1X oo ap 0 JJ DH to Waste 26782 4297 10046 fo 15 39 3 Hopi 1907 38 se nei aa Summe 373 48 32 3466667 1577466667 24 0533333 3 EE ENES PER Ke ipu wasi peter 2297 38 2 z041 2721333333 250 3333333333 1809 0 13568 180 9066667 13567 1808933333 4 E O GE 1946 3244 b5435 pf 1409 Dhiowaste 16662 2777 770 12 ma fe _ ee s ei x E AN O O O O j esh to EC 969 6 5083 67 77333333 94611 126 1466667 resh to Waste 10 oi 08 3 0 o DH to HU 2 DH to EC w I Hei N EIKE o I nn Np ayo mim Ti D ao el ak S z 5 KO N 7 1454 m v m m x S Di pau 9 E 2 an mil 2 R 25821
9. ECDK only for electrical connection of EC Test sequence No Step Result 39 Weigh Water Reservoir 1246 4 g 40 Weigh EC 1729 7 g 41 Mount EC to Rotor I F and turn it accordingly Ok 42 Connect EC connector Ok 43 Start ECDK recording Ok 44 Start external air pump with by pass to achieve a flow of about Ok 180 200 ml min set voltage approx 2 9V ECDK 45 Switch EC Power ON ok 46 Set Digital Input 4 0 4 at ECDK 3 at FM etc ok 47 Set Digital Input 5 0 ok 48 Set Digital Input 4 1 ok 49 Set Digital Input 4 0 ok 50 Set Digital Input 4 1 ok 51 Set Digital Input 4 0 ok 52 Set Digital Input 4 1 ok 53 Set Digital Input 4 0 ok 54 Set Digital Input 4 1 ok 55 Set Digital Input 4 0 ok 56 Start p sensor recording ok Next steps will be repeated until p sensor triggers 57 Note pulse no in Pulse Record below ok for each pulse 58 ECDK Set Digital Input 2 1 ok for each pulse 59 ECDK Set Digital Input 3 1 ok for each pulse 60 ECDK Wait 10 seconds then read Analog 3 and note ok for each pulse triggering at itin Pulse Record if pressure increases gt 1V exit pulse 56 the loop 61 ECDK Set Digital Input 3 0 ok for each pulse 62 ECDK Set Digital Input 2 0 ok for each pulse 63 GSE Valve Switching tab ok for each pulse switch valves B1V4 and B1V6 ON 64 GSE Pump Swi
10. Integration Requirement Document ADO4 N USOC PRO 002 20 06 07 1 3 Multigen 1 PCC Assembly and Seed Set Up Procedure AD05 N USOC PRO 004 16 01 08 1 CW RW Seed Set Up Procedure ADO6 EMEX RP MULT 02 EADS 11 12 03 2 EMCS Experiments Multigen Part 1 Design Report EADS ADO7 RP 30193 PR 02 06 10 05 EMCS Experiments Multigen 1 Design Report CCNO4 Prototech AD08 EMEX MA MULT 01 PRO 14 12 07 E EMCS Multigen 1 PCC 1 User s Manual 1 2 Reference Documents RD Document Number Date Issue Title N USOC TR 027 CW RW Issue 1 PCC Assembly and Seed Set Up Report Date 13 02 2008 Page 4 of 105 2 List of Abbreviations Applicable Document Experiment Container Flight Model Hardware International Space Station Japan Aerospace Exploration Agency Norwegian User Support and Operation Centre Plant Cultivation Chamber Principal Investigator Quality Assurance Reference Document N USOC TR 027 CW RW Issue 1 PCC Assembly and Seed Set Up Report Date 13 02 2008 Page 5 of 105 3 Summary The CWRW PCC assembly and seed set up was performed at the Plant BioCentre in Trondheim Norway from January 14 through 24 2008 This document and its appendices cover the growth pot sterilization filling of growth support set up of seeds implementation of immobilization membrane PCC assembly and mounting of EC cover 4 Introduction The objecti
11. Norwegian User Support and Operations Centre Applicable and reference documents Applicable Documents Doc Number Issue Date Rev Title ADDI USOC MEQ GU 06 0707 L EMCS CWRW Integration Requirements Document AD02 N USOC ICD 004 D N USOC to CW RW Interface Control Document ADOS N USOC MAN 004 31 03 06 EMCS Ground Models Usage Process Plan ADO4 EMEX RP MULT 02 11 12 03 EMCS Experiments Multigen Part 1 EADS Design Report EADS AD05 EMCS MA 4000 002 12 11 04 2 EC User Handbook DCR AD06 RP 30193 PR 02 06 10 05 EMCS Experiments Multigen 1 Design Report CCNO4 Prototech ADO7 EMEX MA MULT 01 P 15 05 06 D EMCS Multigen 1 PCC 1 Users RO Manual AD08 EMEX PR MULT 04 P 05 12 06 A Procedure for filtering of Zeolite RO A CW RW Seed Set Up ADO9 N USOC PRO 004 16 01 2008 1 Procedure AD10 POIF 1005 Oct 2007 C Payload Operation Handbook Volume 2 AD11 N USOC MAN 004 31 03 2006 D2 EMCS Ground Models Usage Process Plan Reference Documents RD Document Number Date Issue Title RD01 Test N USOC TR 25 08 01 2008 1 EMCS ERM CW RW Test Report Biocompatibility Germination Test RD02 Test N USOC TR 28 12 02 2008 1 EMCS ERM Cell Wall Resist Wall Test Report Science Test RDO3 Test N USOC TR 29 26 02 2008 1 EMCS EM CW RW Re watering Verification Test Report RD04 N USOC NOT HS 002 15 02 2008 1 PCC Watering Strategy for the CWRW Experiment RD05 N USOC PRO 005 27 02 2008
12. issue 2 WRarweslas Brat Lassari and Auerations Cast x and Check Out Procedure Date 27 02 2008 Page 48 of 58 APHIS Animal and Plant Health Inspeotion Service Protection amp Quarantine United States Department of Agriculture Animal and Plant Health Inspection Service 4700 River Road Riverdale MD 20737 Permit to Import Plant or Plant Products For Exp erimental Purposes Departmental 1 This permitwas generated electronically via the ePermiis system PERMITTEENAME Tom Erman PERMITNUNBEE PDEP080004 ORGANIZA TI ON NASA APPLICATION NUMBER P588 080219 004 ADDRESS CAPPS Warehouse Bldg 5 698 DATE ISSUED 02 19 2008 867 2199 Ivhil StopSK FL Kennedy Space Center FL 32899 MAILINGADDRESS CAPPS Warehouse Bldg M6 698 867 2199 Mail StopSK FL Kennedy Space Center FL 32899 PHONE 321 867 2199 DATE REVISED 02 20 2008 FAX 321 867 7108 EXPIRES 02 19 2009 PORTS OF ENTRY FL Orlando Under the conditions specified this permit authorizes the following Countries of Origin Plant Paris Grown m U S Intended Use Seed No Analysis of plant response to micro gravity onboard the International Space Station SPECIAL INSTRUCTIONS TOINSPECTORS NONE Pe mit Number P DEP OSDOS THIS PERIVIT HAS BEEN APPROVED ELECTRONICALLY BY THE FOLLOWING PPQ DATE HEADQUARTER OFFICIAL VIA EPERMITS Karen Brady 02 20 2008 WARRING Anyalontbn forgsyox wnvuftorirol wo of f Fodaral Form y c ubpctte chil p
13. 1 SCOPE EE E EEDE EN SE D REYON 45 2 REFERENCE DOCUMENTS TTT 6 3 PARTICIPANTS REQUIRED T REN MEE 7 4 EQUIPMENT LIST SEENEN KA ees ge hEe Nee 8 5 DESCRIPTION E 9 5 1 Equipment an a an Rechen esas ck leere PERSE TA FEE YD 9 5 2 Filtering of Zeolite uu UTO erra er ae a baa Rd No RF ER AOR Cad eo eR Veg du 9 6 STEP BY STEP PROCEDURE uannnnnensvennnnennnnnnnvnuesvnnusvennnnensennnsovsneansnenovnnnnen 13 7 ACCEPT REJECT CRITERIA e ka kk an RESAN PREN sasa sd an ENN EE 15 8 DEVIATION aannnvnunannenevnnnnnnnnennenennunnannvevnnnvnnanenenununnnnnnnannsnansunnsuennnenununnnnenen 15 9 NONCONFORMANCES nseennnnenenunanunnnenntnnnnnsnnnnevnvnnnnnannsnnenunsnvnanumnnnenenvanennene 15 10 PROCEDURE SIGN OFF SHEET orrnsuennnnvnnvnnnnnnnnnnunnevunvvnensnsunenneusneennnnnvenennnnn 15 11 TABLE EIST er m 16 APPENDIX A PROCEDURE DEVIATION SHEET O 17 APPENDIX B NONCONFORMANCE LIST 18 APPENDIX C PROCEDURE SIGN OFF SHEET uuenensannnnnnnvennannnnavunannnnnnnnznnnnnennner 19 The information contained herein is proprietary to PROTOTECH AS and is to be used by the recipient solely for the purpose of which it was supplied It shall not be disclosed in whole or in part by any other party without the written permission of PRO
14. 5 von of 5 4 RECOVERY For RBLSS FM008 the QD repair was already done during the trouble shooting activities The following actions are still to be made e Exchange of one QD at RBLSS FMOOS thread is slightly worn e Repair activity for RBLSS FM007 according to RD8 5 APPENDIX REPORT ON TROUBLE SHOOTING OF NOT PROPERLY HYDRATED ECS SPR 0283 EMCS RP 0000 151 DOR iss 1 Trouble Shooting for Dok Nr No EMCS RP 0000 151 DOR Ausgabe lssue 1 Datum Date 18 8 2008 SPR 283 i Seite Page 1 von of 24 Titel A ge Trouble Shooting of not properly hydrated ECs SPR 0283 Title Konfigurations Dokumenten Typ Nr Document Type Configuration Item No Referenz Nr ete SEH Classification No Lieferbedingungs Nr Freigabe Nr DRL DRD No Release No Gruppierung Gruppierung Dok Version Group Doc related Group Version related Thema Subject Kurzbeschreibung Abstract Autor Org Einh Unternehmen Prepared by R Botn W Bi mann Organ Unit TO53 TOQ 5 Company YO Astrium 4 eege l Gepr ft i 4 Org Einh Unternehmen Agreed by Nee 4 f Organ Unit TO 53 Company EADS Astrium LA Gepr ft LA I LB Org Einh Unternehmen Agreed by W Biema 4 g X oY Organ Unit TOQS Company EADS Astrium Genehmigt FHN local ECB Chairman Org Einh g Einh Unternehmen Approved Organ Unit TE Company EADS Astrium by R Bosc E 43 4 08 is Dok
15. Cawg si Je eseg oo uoneJ6eyu y oj PEYDENE aq eys EUUN4 00L uonej6eju y Ing euuny IooL uogej8eju ou swous vonesjsnif ee yousq mol ejuejs ui pauuouad eq Isny Ru Od UMOJS eui jo ped Jaddn oun yo BueueAo eBpe eu Japun AJA JA JA JA A penu AjnjeJeo eq eus 3eJ dd Sy jo eBpe eu eoeuns yey ueld peuuojoeg iBuoM ojoug ouieg uonduosaq days 95 JO S efeg I WO p age 2002 S0 z oe A j au Buiqinisip 3nouyM ejjoez pesselduioo ayy jo doy uo Ajinjeveo A ATA Wee DU SPP werd eu geld Deg erene eui Woy oJ dd eui srowey CL enss eynpe2oJg dp jes pass pue Ajquiassy 99q L ueBniniy Z00 Odld DOSN N soueunssy Ayyenr uoneao em A0 pauuojieg 500 PUB ROMA pako whol S Vd so lo 99 IO bl PN Yoyo AJAIAJAJA ALATA ATA JA FAVA TA TALA amp p Z Ala la la al NATA ka pawuopad yieuu q oloudq ouu q OR uoueq mop ay ap s no pawuopyed eq ueg pigoqpieo jo asad e uo yi eaejd pue dn epis dn pij un L gt youaq MOJ 84 episjno peuuoned eq ueg alu Jayem wou Guu o jews OSS asc BAOWAY einjeds e JO een Aq uorss 5 1 SY ozu umop pessaJd eq AjinjeJeo 3snu pue uoisseoai ay Ley JeBue LIL S si ejeorisojoq SUL PIT 22d 9u jo xoeq eu uo uoisseo8 au ui ejeaiisoJoq jo 1eeus Jenou eoejg pouwWey jou si POY eduelsaloy auns ayen uoueq Buom u uo Dr umog spis dn
16. Project 30193 EQUIPMENT LIST Page 8 of 19 4 EQUIPMENT LIST Doc no EMEX PR MULT 04 PRO prototech 4 EMCS Experiments PR 30193 PR OS Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 Description Page 9 of 19 5 DESCRIPTION Filtering of Zeolite No particles below 0 5mm in diameter 5 1 Equipment Filter jig FJ Filter jig with Propyltex PP felt with mesh opening of 0 5mmx0 5mm SEFAR PP Felt article no 05 500 36 Container 1 C1 One container same size or bigger than Filter jig collecting the Zeolite particles below 0 5mm in diameter Container 2 C2 One container or glass for collecting the filtered Zeolite 5 2 Filtering of Zeolite 1 Place C1 on a clean table and place FJ on top of Ci As shown in picture below The information contained herein is proprietary to PROTOTECH AS and is to be used by the recipient solely for the purpose of which It was supplied It shall not be disclosed In whole or In part by any other party without the written permission of PROTOTECH AS no EMEX PR MULT 04 PRO Int Doc no PR 30193 PR 05 Doc 2 g E 2 x wi u O z LI prototech 03 05 2007 B Date H Issue Procedure for Filtering of Zeolite iption Descr See pictures below Pour unfiltered Zeolite into FJ do not filter to much Zeolite at a time 2 purpose of which it permission of PROTOTECH AS pient solely fo
17. USYM Jojou sij uo 6x uni oj asuas yew jou saop 1 3 3 E z z o o LLO MH UVd vOLLIO p lu uu duuj pue Juswow ey je Jojou y y uo ave sBui pees payeulwueb Ajuo ayi t HOISSUUANS uonisod UVd Pale 2Y snjvis EP md m AT JOJEUIS HO ISULI 10 UOSBIY aduBtyI JO UONdLDSag 3 di DH YL JO p bed 1517 Wa uonoy Bun N sseuipees youne snqunjo9 se e9 jue d y MOO D I Od 24 uo u s eq ue souapuog UE SUOD o pg u eje UONE USA eu eseajou ZELLN ZELLND SELLNS day Id S p lu uu lduu ZELLIN ZELLWO EE LU day Id Sp lu uu duu v LLINO VELLWNO PELLWO day la p lu uu duu LELLND OE LLIND 67 LLND day Id pajueuie duu VXV q pe o1dde u q e eu SJUSUISAOL sABa a aso jo uogeAjesqo juanbay ja L Uabyiinin UNM uoneioqeloo Jo Hed sy Bunse ueu Jo swa s AIB pue sseooJd YMoub ay dn peeds Ajinjadou Wu Siu Ajsuejui N ejeJ9 oj UEO pue pjo skep Oz Meou ale pg ui sjueld MOU 00 inoqe je Apuejsyon SI euinjoA 93 ay episul Ajpiunu y pue Biq eynb aie sjuejd ay 1 vg ui sjuejd jo uoneAjesqo Ju nb J jueurejdui lin o paonpay wou Aisuayu yi aseasouj pg ui Bunu A peseajoui jueuje duj LLO MH7HVd LELLIND OE LLND 6cLLWO dey Id Jejem ap 0 woy Bunayns Ing eje aq Wis BW LE ur sjuejd ayi einpayos p lu uu lduuj LVMLINI e a sas nd pide 00 Dua asojaq as nd Huo sem p euo uebe 1g jo U
18. Up Procedure ki B ot S9 4 Ground Support Requirements Table 1 shows the Ground Support Requirements needed to set up the PCCs for flight Table 1 Ground Support Requirements for PCC set up for flig Description Large workbench Sterile flow bench Balance Capacity 3 kg accuracy 0 1 g Ethyl alcohol 70 Ethyl alcohol 96 MULT 1_GSR_07 Spray bottle Stereomicroscope Pincers with micro tip medium and large Disposable gloves Sterile filter paper for drying of PCC sponge Exsiccator Short glass pipette Sterile pipette tips in pipette box 1 box MULT 1 GSR 15 Pyrex beakers 8x50 ml 12x250 ml 2 MULT 1 GSR 16 Sterile water Chiorine 2596 2 di Eppendorf autopipette 10 25 ul range 1 Autoclave bags Bensen burner MULT 1 GSR 25 Card board for cutting of borosilicate MULT 1 GSR 26 Small Spatula MULT 1 GSR 27 Aluminum foil Available l d wl al of of Al n A wl a mh box lt gt lt e i I e 1 box a 3 i N USOC PRO 002 Multigen 1 Issue 1 3 PCC Assembly and Seed Set Pate 20 06 2007 Up Procedure Page 9 of 56 5 Set Up Articles Table 2 shows the articles that are needed to set up the Multigen 1 PCC for flight Table 2 Set up articles Test Article name Supplied by Multigen 1 Flight Model PCCs EADS Growth medium Zeolite per PCC Diameter of PP Felt per PCC Diameter of Borosilicate per PCC Polyvinyl
19. astkrium Dok Nr No EMCS RP 0000 151 DOR Trouble Shooting for SPR 283 Ausgabe lssue 1 Datum Date 18 8 2008 Seite Page 4 von of 24 2 3 4 2 Characteristics of returned RBLSS Switch off Breadboard E Box Switch off Rotor computer Trend Type at Keyboard lt Alt Strg Entf gt lt Enter gt lt Enter gt lt Enter gt lt Alt F4 gt lt Enter gt Press computer button off Switch of GSE Computer Hyundai 4 2 1 Sucking from Waste Water was pumped from waste water reservoir through RBLSS to the RBLSS rotor I F QD01 resp QD04 QD opened by counterpart As to be expected in consequence of the swapped QDs no water was supplied Path 6 Waste gt V1 ON PW1 ON V2 OFF V3 ON gt QD01 opened by counterpart QD Waste gt V4 ON PW2 ON V5 OFF V6 ON gt QD04 opened by counterpart QD No Test Water Reservoir RBLSS Module Note outlet outlet 1 water service path 6 FM009 QD10 FM008 B2 QD04 flight configuration except Rotor I F and EC 2 water service path 6 FM009 QD09 FM008 B1 QD01 Waste Water Reservoir pressure Figure 4 1 Setup for water supply test with wrong QDs No Step actual value 1 start EC water service 1 pulse behavior as expected no delta pressure no water 2 start EC water service 5 pulse behavior as expected no delta pressure no water 3 start EC water service 3 pu
20. e2uenssy Ajjenn UONEDOT ea Aq pauwuopad to PARIS Laat PALE uo jwe 9010 Hl MAG UOH m gt nanvor IAU lg aGuods ayi ajexij 01 umop Burysnd asojaq Buys ayi JO uonisod au sAsasqO 10 4 YMO BU 0 AHE J ABM 08 1100 oou eT E eu SSE y ans ayen oujeuiul s jou SI Bury uonexiJ SUL x i Bury uonexiy Sy jo apisyno SY UO WW e gisi 8q jeus 249 uwelp SYL Uonexuy JO Suu jod umob Duupeug eJojeq 3210 jews Aq no AjueAe u9jens pue JESUS YAd JOM UJ uionoq Jod YMOID JeA02 ejejduis se JOY MOIH ay JO ulli Jejno esf oued yos e ui JESUS vAd 94 uo a 241D e MEIG Jayem ssaoxe UE no azeanbs pue Aju6noJouj jeuiejeut jan ww g z sseuxoiuj eene jeeus vAd joyoojy UIN JOd JO Wa 9x9 Ajeyeuixouddge eoeid e aye X18utra J 030ud uoneJjsuouied uonduosag deis eJnpeooJd jo uonduosep days Aq dajs 1 ejqelt ZO0 ONd OOSN N 898 siiejep 103 30d YIMOID 29d Jequieu vongann9 jueig L ueBnjn w eui JO Wed woyog ay ul eBuods y jueulejdui o MOU sequosep einpeooJd Buiwojjo eu Jod YIMOID JIJ uo eBuodsg Jo uonexiJ L 95 JO GE afed 100z st ez aen Jod YIMOID JIJ uo eBuods jo uonexi4 CL enss xipueddy infi PE vonesoubp 00 OMd DOSf N LIN 92ugunssy Ayenp uoneoo eg A0 pauoyag bbo P SWA PS VIG Sid 89 Io C pwe go lo h eng stet c Wi SU 0 so 5 UB pue SI ye AjuBno jadjeos A0 jena eBuods ssaoxe esoo ing psunoyad YleWaY OJOUA4 UONENSUOWag uonduasag dag quo Lage 96
21. identified Impact on experiments that use the watering feature of EMCS See Appendix 7 for details Initial Hydration EIR Exec 4 EIR Exec 5 EIR Exec 6 Experiment duration EIR Exec 7 Main Experiment objective 1 Tried to add Water pulses by manual cmds 2 Tried to use gravitational force to ease the hydration 3 Tried to recycle water from dehumidifiers to EC 4 Tried exceeded pumping time to force away eventual debris 1 Germination was seen in EC FM092 FM098 and FM100 2 The workarounds could not support the seedlings and early harvest was required Gravity level EIR Exec 13 Illumination EIR Exec 15 Harvesting EIR Exec 17 EIR Exec 18 EIR Exec 19 Main Experiment objective EMCS HW 0013 Zero Airflow in EC B3 The water outlet filter of EC B3 was blocked due to too much water in the bottom chamber in the CWRW growth pot Main Experiment objective After the water had evaporated the airflow could be restarted after 5 days and remained nominal The flooding of EC B3 FMO98 led to drowning of the seedlings in this position Main Experiment objective Samfunnsforskning N USOC Norwegian User Support and Operations Centre 4 2 Altered Requirements EMCS CW RW Requirements Integration Report Doc Nr N USOC REQ 025 Issue 1 Date 28 11 08 Page 26 of 30 From the 31 Requirements listed in ADO1 for pre experiment and experiment
22. uhr N USOC PRO 005 N USOE m CW RW Flight Sample Hardware Packing Transport issue 2 Rerwtalss Bun Langer und Rotritions Casiri and Check Out Procedure Date 27 02 2008 Page 53 of 58 NTNU N USOG T Social Research Ltd Borwrgira Beer Sugar amt Operations Centre Dated 15 01 2003 Our ref N USOC NOT SE2 004 Your letter dated Your Ref Shipping Packing List Transport to Transportation officer CAPPS warenouse Bldg M6 698 Kennedy Space Centre FL 32899 Mark for Tom Erdman 867 2199 Mail Stop SK PL Phone number 321 867 2199 321 867 661 1 No cm 44x32x15 7 33 Seeds of Arabidopsis thaliana Inside the Plant Cultivation Chambers PCC 11 Wild Type seeds 7 GM GUS seeds 15 Mutant Lefty seeds 3 Experiment Containers EC with installed Plant Cultivation Chambers PCC CI No SIN FMD92 FWO94 FMO95 44x32x15 7 33 Seeds of Arabidopsis thaliana I Inside the Plant Cultivation Il Chambers PCC 11 Wild Type seeds 7 GM GUS seeds 15 Mutant hmg seeds w Experiment Containers EC with installed Plant Cultivation Chambers PCC CI No SIN FMO96 FM 98 Seeds of Arabidopsis thaliana Inside the Plant Cultivation Chambers PCC 8 Wild Type seeds 15 Mutant Lefty seeds 15 Mutant hmg seeds n Experiment Containers EC with installed Plant Cultivation Chambers PCC CI No SIN FM099 FM100 Adress Location Tel 47 73 5
23. 06 i 4 ECin EC out E ECin EC out eR E I manna Sel Seal TT mamal self sealln mes Ben ne ID automatic t self sealing EC Position 4 EC Position 3 eu 02 um Filters 02 um Filters RBLSS Box 2 a Identical to Box 1 x i TE fel fe wo i m opos y y bo Qno QD QD 02 x Q ov osfy jap os i eu T EC out d EADS gcbriL i m Dok Nr No EMCS RP 0000 151 DOR Trouble Shooting for SPR 283 Seite Page Ausgabe lssue 1 Datum Date 18 8 2008 6 von of 24 4 2 2 Pumping into Fresh Air was pumped into fresh water reservoir through RBLSS via RBLSS QD12 S into Fresh Water Reservoir As expected by the swapped QD failure the water bag containing trapped air volume was blown up and the pressure increased by compressing the air in the bag Figure 4 4 air GSE pressure Fresh Water Reservoir Figure 4 3 Setup for water removal test with wrong QDs QD10 N opened by counterpart gt V1 ON PW1 ON V2 OFF V3 OFF gt QD12 S gt Fresh overpressure in water line mbar No Test Water Reservoir RBLSS Module Note outlet outlet 1 water service path 6 FM009 QD10 FM008 B2 QD04 flight configuration except Rotor I F and EC Pumping air through wrongly connected QDs into fresh water bag Water Reservoir FM009 RBLSS Module FM008 pump switched off at 700 mbar 800 4 700 o e 0 13 55 12 14 09 36 14 24 00 14 38 24
24. 14 52 48 15 07 12 15 21 36 15 36 00 15 50 24 16 04 48 16 19 12 16 33 36 Figure 4 4 Pressurization of fresh water bag when pumping air into it simulation of what happened during CWRW when A1 A2 B1 B2 dehumidifier were drained d Dok Nr No EMCS RP 0000 151 DOR EADS Trouble Shooting for SPR 283 Ausgabe lssue 1 Datum Date 18 8 2008 as E rium Seite Page 7 von of 24 4 3 Water supply to EC 4 3 1 End to End Test with EC FM092 Setup according to Figure 4 5 EC FM092 has no PCC mounted Therefore a syringe is installed to collect fresh water entering the EC Initially the EC is not mounted during filling of lines The Water Reservoir is oriented with fresh water bag upside keeps air bubble inside bag Water Reservoir EC Rotor I F pressure Figure 4 5 Setup for combined test with EC092 without PCC Test Water Reservoir RBLSS Module Rotor UE EC Note outlet outlet Adapter water service FM009 QD10 FM008 B2 QD04 S N 10 FM092 without PCC syringe path 6 as reservoir Test Equipment Test Sequence Rotor UE adapter S N 10 adjusted to worst case tolerances low interface high rails pressure sensor tube kit RBLSS GSE OHB No Step Result 1 Fill water lines to EC by pumping water from Water Reservoir ok 3 pulses 4 ml needed to fill via RBLSS to Rotor UE adapter the septum will be opened with the septum push tool un
25. 304 i L mv vm m HUD fn z2 og m De O 0000 RY ELE reshtoHU ve s of of 7 2566667 reshtoEC 4590 3060 ara 22526667 1399 18 5535335 rshioWase of i2 of of of 70 0395335833 0 93339333 DHtoHU 3 i l eg o sy 2 4069 dos me 7 naj 18 j ST EE Eee ERE 7 x p of 20 3322666667 281 j 4me 5634666567 T O of 7 0395353353 As O Z T 98 l5 379 6 100 se Zo o ss Summe 267 0938333 15 3460007 EE nm s iS I N s A o o i M En co HU to DH resh to HU resh to EC resh to Waste DH to HU 24 42 eo 1 PM no DIO DIO 26 ao ce W N 2277 123 ojlo mm DIE TI EI SEDE pm ojo ojo m m 5 0 15 a a o o is Dok Nr No EMCS RP 0000 151 DOR Trouble Shooting for EADS Ausgabe lssue 1 Datum Date 18 8 2008 asEer um SPR 283 Seite Page 22 von of 24 7 RBLSS MODULE FM 007 7 1 Inspection and Functional Test A major discrepancy was found during incoming inspection at KSC of items returned on 1J RBLSS Module FM 007 Quick Disconnects QDs towards fresh and waste water reservoir were swapped meaning e QD 10 N Fresh at RBLSS was equipped with a female QD thus fitting to waste QD of the water Reservoir e QD 12 N Waste at RBLSS was equipped with a male QD thus fitting to
26. A Procedure for filtering of Zeolite ADOS EMEX RP MULT 07 EADS 01 07 2004 EMCS Experiments Multigen Part 1 Interface Specifications AD10 EMEX RP MULT 10 EADS 11 12 2005 C EMCS Experiments P L Specification Multigen Part 1 2 2 Reference Documents EMCS Experiments N USOC PRO 002 Multigen 1 Issue 1 3 PCC Assembly and Seed Set Date 20 06 2007 Pi 7 Up Procedure so orp 3 Multigen 1 Experiment Design The Multigen 1 Plant Cultivation Chamber PCC is accommodated inside the EMCS Experiment Container EC as shown in Figure 1 EC with cover Figure 1 Multigen 1 Experiment implemented into EMCS EC The PCC constitutes the Experiment Unique Equipment of the Multigen 1 Experiment The PCC will maintain enclosed inside the Experiment Container EC at all times while on the International Space Station The seeds will be implemented in the PCC and the PCC will be integrated in the EC on ground prior to handover to the National Aeronautics and Space Administration NASA Once implemented into the European Modular Cultivation System EMCS on the ISS the PCC with seeds will be hydrated automatically The plants will automatically be dried out at the end of the experiment removed from the EMCS while still inside the enclosed EC and dry stowed until return to ground N USOC PRO 002 Intcyatias avd Creativity Muitigen 1 Issue 1 3 PCC Assembly and Seed Set Date 20 06 2007
27. Alcohole PVA per hole 10 ul Available 1 gt PVA Moulding Tool 1 7 Integration Tool Base Prototech Integration Tool Funnel with screws 2 Prototech Integration Tool Stamp Prototech 10 Mini Lid Attaching Tool 11 Borosilicate Punching Tool 2 12 Pi x Up Side Down Jig for PCC Lid 6 Environmental Conditions during Experiment Set Up The ambient conditions within the laboratory for filling and assembly shall be in compliance with the values specified in the table below it is recommended to keep the air humidity as low as possible to avoid early germination of the Arabidopsis seeds The actual values shall be filled into table 3 N USOC PRO 002 Multigen 1 Issue 1 3 PCC Assembly and Seed Set Date 20 06 2007 Up Procedure Rage 10 0S8 pressure and cleanliness during experiment set up Room temperature 22x3 C 32 Q 5 25 e a Table 3 Temperature humidi Cleanliness Sterile Flow Bench Geriu Flow Buch eoueinssy Aen uongeoo1 je A0 psunopad way vl Std 201067 SOL SI VID 797 sanul 09 JO 2 04 18 jeuiqeo ezijuejs yeay ur e ejd pue jur OG Jeyeaq XeJAg 0 eyjoez Deet J JSUEIL SARIS YJBSUJSPUN Jeueajo winnoea e Duped Aq unu g o SAOGE yoq Woy seed 1SNp Jeuonippe saoway WLU g o UBL ssej azis uM sapped Plone o JepJo ui Z xipueddy godv einpeosoud o Buip1022e eyjoez QENIA eiuounuy moyym wntpauw SIN L xipusddy 0 Buipioooe Lu
28. Commissioner of Customs lo be imported for the use of the National Aeronaulics and Space Administration or for the implementation of an intemational program of the Nationa Aeronautics and Space Administration including articles to be launched into space and parts thereof ground support equipment and uniquely associated equipment for use in connection with an international program of the National Aeronautics and Space Administration including launch service agreements S Note 1 of Subchapter VIII of Chapter 98 With respect to Subheading 9808 00 80 goods brought into the cusloms terntory of the United States by the National Aeronautics and Space Administration from space or from a foreign country as part of an international program of the Nalional Aeronautics and Space Administration shall not be considered an importation and an entry of such materials shall nol be required March 3 2008 To Customs Officials Transportation Security Officials Suzanne Overlie employee from Norwegian User Support amp Operations Centre in Trondheim Norway will be delivering N USOC NOT SEO 003 experiment hardware as identified on attached invoice which is needed as part of the European Modular Cultivation System EMCS facility which will be used to perform biological life sciences experiments The N USOC NOT SEO 003 experiment hardware is scheduled to fly aboard STS 123 Endeavour on International Space Station Mission 1J A with an anticipat
29. EMCS CW RW Requirements N USOC Me Integration Report Norwegian User Support and Operations Centre Samfunnsforskning AS Doc Nr N USOC REQ 025 Issue 1 Date 28 11 08 Page 1 of 30 EMCS CW RW Requirements Integration Report N USOC REQ 025 Issue 1 Date 28 11 2008 ESA Contact Reference ESTEC Contract 18279 04 NL SH Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 N US0C m Integration Report Date 28 11 08 F Page 2 of 30 Samfunnsforskning AS Norwegian User Support and Operations Centre Prepared by Suzanne verlie N USOC Integration amp Operations Engineer Checked by Carina Helle Berg N USOC Integration amp Operations Manager Checked by Knut Olav Helleseng N USOC Systems Manager Checked by Knut Robert Fossum N USOC Manager Checked by Ulrich K bler EMCS CWRW Payload Integration Manager PIM Checked by Toru Shimazu CW RW PI rep Contract Ref ESTEC Contract 18279 04 NL SH The Plant Biocentre Department of Biology Norwegian University of Science and Technology NTNU 7491 Trondheim Norway Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 E Integration Report Date 28 11 08 N US06 k Page 3 of 30 Samfunnsforskning AS Norwegian User Support and Operations Centre Internal Distribution Name Affiliation Knut Fossum N USOC Ann lren Kittang N USOC Carina Helle Berg N USOC
30. Jaded pezijuejs ui Jejem sseoxo Jno sseJd Ajinjajeo pue eu yoge Jejem jo efueyoxe saw aem pesiuoiep pezuiijeys ur AjuBnoiou eBuods oun asuny uu OL euuo uo GZ ul Jod ay Bui amp Bieuiqns Aq Buods jog ymous eu eziuajs p A0uu 1 SG UBD jeu Jaysew Aq spij Jod yimoJ6 pue sjod uoi e jagej pue g x pusddy 0 Buipio2oe jog YIMOIN JOd jo ped woyoq o eBuods alexi uonduoseq doe CL anssi ainpss0idg AN Jas peag pue Alqwassy 99d L ueBnIniy Dua pat veel Z00 ONd O0SN N 1400 v SIgEL eoueJnssy PENT UOHE007 eya A0 peuuonag pape PPW Sad gO Q he Bo lat epewnw YEP 7 PSYDBEL si mone UM paye jaaa 3281102 ay pun ejnjeds e YIM 814087 y jo eulos AOWA ane usAID ay 0 UMOP yurs jou se0p 81087 JO 9 9 OU jj peppe eq jsnui ajijoez sow MOLE Ui E m 3 peyew jaaa UEL e mojaq SUIS 91097 JO erg eui J aow Aue U sse1duioo jou seop ejjoez eui jun peuuoged eq eys Burdde syr YOUSG yom y JO eoeuns y Jsule e Jod YMOIS pejeJBeyul uM AA ATA JA LA TALA aseg joo uone bsjuj y Guidde Ajinjereo Aq eyjoez au ssaidwo5 Buun j00 uogeJBe3uj ei gluotuuuv inou j A AJA VA JA JA JA upper SA Id Aq peuouue eyosz uy jog YIMOJS eue at I4 i SMEJOS UYIM Be eseg joo uonei amp eju o jeuun joo uoneiBeju UE 10JEIIISX3 AIALATAIATALAL A T Wout jog ymo papau ym aseg joo uonelBeju saoway peullojlag y18euiosj 0joug oue uonduosaq deis 1U
31. KFT with formaldehyde The KFT with formaldehyde was frozen for return immediately after harvest MELFI to 95 C In total all ECs were removed from the EMCS and stowed see table 3 9 for details Dry mode of the EMCS Facility was performed in the afternoon After three days the two KFTs with RNALater were relocated from 2 C dewar in MELFI to 95 C dewar in MELFI Table 3 9 CW RW Harvest Flight EC A1 Flight EC A2 Flight EC A3 Flight EC A4 EMEB Flight EC B2 EC No FM097 FM099 FM094 FM096 FM092 FM095 FM098 FM100 N A EC EC Stowed Stowed and and later 1 KFT with later returned returned with RNALater Plant on 1J for 1J and and material N A EC troubleshooting N A EC N A EC harvested on N A EC 1 KFT with 1 KFT with to KFT Stowed on ground Stowed Stowed ground Stowed RNALater Formaldehyde RNALater 2 C for three 2 C for days than three days relocated to 95 than C dewar relocated to Formaldehyde MELFI 95 C dewar Directly to 95 DEWAR N A N A N A N A N A NA C dewar The experiment lasted for 47 days but due to late germination the plants were estimated 23 days at the time of harvest For more detailed information on sensory data like temperature relative humidity gas composition etc during experiment execution on orbit telemetry files located on the N USOC Drop box server can be accessed upon request Doc Nr N USOC REQ 025 EMCS CW RW Requirements Int
32. Knut Olav Helleseng N USOC Suzanne verlie N USOC Maja Rostad N USOC Gjert Aanes N USOC H kon Svare N USOC Liz Helena Coelho N USOC Abdul Basit Mohammed N USOC External Distribution Name Affiliation Jens Schiemann ESA MSM E Ulrich K bler ESA MSM E Thomas Niedermaier EADS Achim Schwarzwaelder EADS Pierfilippo Manieri ESA Toru Shimazu JSF Doc Nr N USOC REQ 025 Samfunnsforskning AS EMCS CW RW Requirements Issue 1 Integration Report Date 28 11 08 N USOC p Page 4 of 30 Norwegian User Support and Operations Centre Summary The CW RW Experiment was conducted on the International Space Station from March 30 2008 through May 23 2008 Several seeds in 3 ECs germinated in the CW RW Experiment on orbit but at the end most seedlings had wilted and only four plants in one EC barely survived until harvest From the very start of the experiment a problem with watering was evident Failure analysis of the hardware which was returned on 1J has in retrospect shown that faulty connections of the Quick Disconnects QDs on 2 RBLSS modules most likely were the cause of the watering issues Due to the watering problem which either led to a complete lack of germination or poor growth of the seedlings the PI Team decided to change a number of Experiment Requirements like illumination watering and gravity stimuli The Operations Team tried as far as possible to fulfill the Pl Team s changed re
33. Norwegian User Support and Operations Centre PY EMCS CW RW Requirements Integration Report Doc Nr N USOC REQ 025 Issue 1 Date 28 11 08 Page 25 of 30 PAR Description of PAR and Impact on science and CW RW Requirements ADO1 Affected Requirement Corrective act and Work arounds during CW RW Impact on CW RW after implemented workarounds Additional requirement affected after work around EMCS HW 0010 Experiment Container B4 Not Responding Correctly The digital input line to EC B4 could not be switched to HIGH at the beginning of the CWRW experiment The implications were that it was not possible to set the Plant Cultivation Chamber PCC accommodated inside the EC in Watering Mode It was still possible to supply water to the EC but it was not possible to use the PCC s delta pressure sensor s triggering point to detect the water saturation point of the growth pot Initial Hydration EIR Exec 4 After a power cycle in the frame of the crew procedure EMCS water flow checks the digital line was working nominally again and failed later again A checkout of this digital line shall be performed with an EMCS EC with reference insert Although impacted by PAR EMCS HW 011 B4 EC FM100 was the most successful regarding plant material EMCS HW 0011 EMCS ECs Not Hydrating Properly Incorrect mounting of Waste and Fresh Water Quick Disconnects on RBLSS Modules FM007 and FM008
34. Nr No EMCS RP 0000 151 DOR Trouble Shooting for EADS ae SPR 283 Ausgabe Issue 1 Datum Date 18 8 2008 gStErium Seite Page 2 von of 24 Daten Dokument nderungsnachweis Data Document Change Record DCR Ausgabe Betroffener Abschnitt Paragraph Seite nderungsgrund Kurze nderungsbeschreibung Issue Affected Section Paragraph Page Reason for Change Brief Description of Change first issue Table of Contents 1 INTRODUGTION EE 3 2 REFERENCE DOCUMENTS sss tnnt ener se ao se ao on ao os aoosaoosanasanoseeossanosssossossonanosannn 3 3 RETURNED TEMS u a ia anne ah nur euch 3 4 COMBINED TESTS WITH RBLSS EC WATER REGERVOIR nn 3 4 1 RBLSS GSE Start up Sequencee as aaa enne enne nnn sen trte enn nnns sn trn inen 3 4 2 Characteristics of RBLSS as used with wrong Quick Disconnects ss ssseesssessressressrnssrnssrnssrnssrnsens 4 4 2 1 SUCKING from Waste ii ai en 4 4 2 2 PUMPING INTO Fresh uya eki an a a oak atak W aa pak pp ek ko es an Ra seen DS aa L Ra 6 43 Water Supply to EE 7 4 3 1 End to End Test with EC FMO92 uesseenseessennsnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnennnennnenn nennen 7 4 3 2 End to End Test with EC EM 9 5 EXPERIMENT CONTAINERS sse aa saaa on aa an aaa rente ih rr sn resi tren tene nen nnt dtr nnn nnns 12 5 1 EG OS e u u ttanko oak on t kwake Miky d ka 12 5 1 1 Before opening bottom plate A 12 5 1 2 Bottom plate and
35. Plant Cultivation Chamber PI Principal Investigator QA Quality Assurance ISS the International Space Station NASA National Aeronautics and Space Administration PVA Polyvinyl Alcohol MS Murashige amp Skoog Medium PP Polypropylene ERD Experiments Requirements Document PD Payload Developer N USOC PRO 002 Multigen 1 Issue 1 3 PCC Assembly and Seed Set Pate RE E Up Procedure Page Sores 1 Scope This document and its appendices cover the Multigen 1 PCC assembly rinsing of Zeolite filling of growth support set up of seeds and implementation of immobilization membrane The document is based on input from Payload Developer PD and Principal Investigator PI The document has been changed since base lining signature Issue 1 2 according to the Change Log on page 3 2 Documents 2 1 Applicable Documents AD DocumentNumber AD 01 EMCS MA 4000 002 DCR Experiments Requirement AD 02 EMEX RP MULT 01 EADS Document Multigen Part 1 AD 03 EMEX RP MULT 02 EADS 11 12 2003 EMCS Experiments Multigen Part 1 Design Report AD 04 N USOC REQ 004 29 03 2007 1 EMCS Multigen 1 Integration Requirements Document AD05 RP 30193 PR 02 06 10 2005 EMCS Experiments Multigen 1 Design Report CCNO4 AD06 EMCS PR 0000 007 DOR 21 06 2006 EMCS EM System Start Up and Stop Procedure ADO7 EMEX_MA_MULT_01_PRO 15 05 2006 EMCS Multigen 1 PCC 1 User s Manual ADO8 EMEX PR MULT 04 PRO A 05 12 2008
36. Water pump run for 1 min then 5 min pause 5 repetitions No change of valve config between the pulses Started hydration schedule 26 pulses given Resat counter to 99 100 pulses 99 26 27 pulses given Sch stopped automatically 98 30 68 pulses given Resat counter to 25 Hydration in 0 8xg started 75 pulses given Sch stopped automaticall Started hydration schedule 95 pulses given Resat counter to 30 99 30 69 pulses given Resat counter to 25 100 25 75 pulses given Hydration in 0 8xg started 73 pulses given Sch stopped manually at this Started hydration schedule with counter set to 24 A total of 57 Could not be hydrated pulses given and dP due to low airflow threshhold reached Started hydration schedule with counter schedule with counter Started hydration schedule Started hydration schedule set to 24 A total of 89 set to 24 A total of 124 with counter set to 24 A with counter set to 24 A pulses given and dP pulses given threshhold reached Started hydration schedule with counter set to 24 Resat counter to 71 when it reached Added water pulses with Added water pulses with 96 Let it run to 100 A total of ADDWATB3 20 pulses ADDWATB4 19 pulses 146 pulses added i Started hydration schedule Started hydration with counter set to 24 A total schedule A total of XX of 90 pulses given and dP treshhold is reached Added water pulses with ADDWATB 1 19 pulses given Started hydration sche
37. a SCODE ES SE OE 8 5 Procedure detailed plan sssscssssssesssscssssssssssccessssencensocsscsessessesessesensccessassacacaceaceaces 9 ST POrsoritigl ccce ie vvv Vo E ANNETTE KES AGNES EN een 9 9 2 ocu m X 10 9 9 Equipment FACE S EE 10 5 4 Environmental Conditions eere eene AENEA noatra ont pe ee nea sae ananas 10 S S DURAN ON k k ES E RRRRMRMMMEMMMMMMWMWWAMMM 10 6 Procedure Description 11 0 1 Procedure EqUIDITIGFIE EEN 12 BA FA uc REE EE ERE 12 8 12 EQUIPMENBLISE EE 12 T Procedure TT EN 13 8 Procedure Variation Fill in scissa a R 15 9 Fallures during Process EE 16 101 Success CPCI EEN 16 11 Proceduro SIGIKO T uen rine rrr neret eben sisan ao nana y FPE eR ERE enke non 17 Annex 1 Filtration of Zeolite as performed prior to Multigen 1 experiment flight set up RIOT Arr A M 18 Annex 2 Certificate of Arabidopsis Thaliana Seeds Separate certificates for Wild Type GM GUS mutant lefty and mutant mg 38 Annex 3 Phytosanitary Certificate for Arabidopsis Thaliana Seeds One combined certificate for Wild Type GM GUS mutant lefty and mutant bmg J JII aa aaar 44 Annex 4 Verification 2 form s esseerensorortrrissnsnnvsnennsannnrnnennrerensenevnevnessensenesesvesnensseseusenensesessnsssvnse 46 Annex 535884 E EE 49 Annex 6 NASA Cover Letter pro forma invoice and packing shipping
38. fresh water bag was emptied and 227 g of water were collected This is compliant with the initial filling volume of 230 g and the analysis of the QD swap where it was derived that almost no water could have been used during operation of CWRW expected value was between 182 and 254 g see Table 6 1 No blockage or any other failure could be found The Water Reservoir was closed again using the original parts and it is regarded ready for filling before launch Figure 6 1 FM001 with waste sponge Figure 6 2 FM001 waste sponge removed 6 2 FM009 No damage was found on housing and QDs After opening of Water Reservoir an air bubble could be identified in the fresh water bag This confirmed that air water was pumped into the fresh bag during dehumidifier draining of the EE for the case of swapped QDs at the RBLSS Module FM007 Corrosion was found inside and outside the waste housing as already known from Water Reservoirs FM003 FMOO4 FM007 EU Dok Nr No EMCS RP 0000 151 DOR NT Trouble Shooting for EADS j Ausgabe lssue 1 Datum Date 18 8 2008 SSErium SPR 283 Seite Page 20 von of 24 The upper housing and fixation nuts have to be replaced by new material and surface treatment as already done for FM001 FM002 see SPR 253 The waste sponge was removed It was found humid and 6 1 g water could be squeezed which is compliant with the analysis of 0 24 g expected for the case of the swapped RBLSS QDs Tabl
39. in switching off the EMCS subsystems occurred due to spikes on the signal of the ACS C2 safety sensor After a workaround developed during MULTIGEN 1 i e decrease the O2 level to 21 95 if above no more triggerings occurred during MULTIGEN 1 After the move to Columbus no more spikes on the O2 signal could be found However one single unexplained triggering of the hardware safety circuit happened during the CWRW experiment Gravity level EIR Exec 13 Illumination EIR Exec 15 Airflow EIR Exec 15 Obs target and freq EIR Exec 09 Power interruptions EIR Exec 31 1 Start of EMCS Atmosphere Control System to reduce the level of PPO2 in EMCS to 21 Samfunnsforskning AS Norwegian User Support and Operations Centre PS EMCS CW RW Requirements Integration Report Doc Nr N USOC REQ 025 Issue 1 Date 28 11 08 Page 23 of 30 PAR Description of PAR and Impact on science and CW RW Requirements ADO1 Affected Requirement Corrective act and Work arounds during CW RW Impact on CW RW after implemented workarounds Additional requirement affected after work around EMCS SW 0003 EMCS VPU Lockup Loss of environmental control and images for 1 2 hours Several occurrences The EMCS VPU failed to communicate with the SPLC The root cause has been identified An EMCS application software update and VPU driver update is required to fix the problem and has been initiated It s
40. main activities related to preparations at N USOC are listed in table 3 4 The related products and performance documents are referenced Table 3 4 CW RW Sample preparation at N USOC laboratory Activity Product Procedure Preparations 8 ECs and 8 PCCs Integration performed by PD at N USOC Set up of 8 Growth Pots growth support and seeds N USOC PRO 004 by Pl Team Implementation of 8 integrated Growth Pots into 8 PCCs N USOC PRO 004 Enclosure of ECs with integrated EUE N USOC PRO 004 Final Leakage check and balancing of the 8 ECs with integrated EUE Verification by IOT Sharp edge 8 ECs with integrated EUE were inspected for sharp edges EMCS RP 0000 131 DOR inspection by HFIT Approved Labels on the 8 ECs with integrated EUE were inspected EMCS RP 0000 131 DOR labeling by IPLAT Preparations at N USOC were performed without any major deviations from procedure The CW RW EUE was assembled at N USOC The PCCs were filled with growth support before seeds immobilization membranes borosilicate doughnuts and mini lids were implemented ADO9 After PCC assembly and seed implementation the EC covers were mounted on the CW RW EC Base plate 8 ECs were prepared for the CW RW Experiment and the CW RW seed and EC Assignment list can be seen in table 3 5 Table 3 5 CW RW EC and seed assignment list as agreed prior to experiment execution p Flight EC A1 Flight EC A3 Flight EC
41. of seeds in EMCS Schedules Water flow check due to lack of hydration Crew Proc Check water flow 4 002 EMCS Water Flow Checks MGUEEXPRSEMCSC002 EMCS Main Door Check Crew involvement Check Main door not closing correctly Water Reservoir Stowage Check Crew involvement Check that the correct Water Reservoirs are installed ERS EMCS Preparation for H2O Crew Proc Turn Power Switches 2 028 EMCS Switch Configuration with Power off Res Replace Off MGUEEXPRSEMCSNO28 EMCS Water Reservoir Replace Crew Proc Replace Water 2 011 EMCS Water Reservoir Replacement Reservoir MGUEEXPRSEMCSNO 1 ERS EMCS Preparation for Crew Proc Turn Power Switches 2 028 EMCS Switch Configuration with Power off restart of experiment run after On MGUEEXPRSEMCSN028 H20 Res Replace EC Swap on Rotor A and B Crew Proc Exchange ECs A1 and A2 with ECs B1 and B2 2 004 Experiment Container Removal 2 003 Experiment Container Insertion Retrieval of CW RW KFTs from Crew Proc KFT Retrieval from 2 003 Cell Wall Resist Wall KFT MELFI Retrieval MELFI pre Harvest MELFI MGUEEXPRSCWRWN003 Set Up of MSG pre Harvest Crew Proc MSG Set up 2 002 Cell Wall Resist Wall MSG Set Up MGUEEXPRSCWRWNOO2 Harvest of CW RW plants OCA message and Crew Proc 2 004 CELL WALL RESIST WALL HARVEST IN MSG Harvest of plants in MSG MGUEEXPRSCWRWNOO4 Insertion of KFTs in MELFI after Crew Proc CW RW KFT Insert in 2
42. place it perpendicular to the Borosilicate Fibre Sheet 2 Penetrate the Borosilicate Fibre Sheet by gently pushing the Outer Hole Punch downwards while slowly rotating the Outer Hole Punch in both directions N USOC PRO 002 AP05 Appendix 5 Issue 1 3 Meuiding of PVA Membrane Date 23 05 2007 apr Page 52 of 56 Porosilicate Cutting lagavation and Lrestevity Norasiza Ere apen Maz Sperscieas estre 3 Keep the punching tool stable and penetrate the Borosilicate Fibre Sheet again by gently pushing the Inner Hole Punch downwards while slowly rotating the Inner Hole Punch in both directions 4 Lift up the tool and confirm that the punched piece of Borosilicate Fibre remains fixed to the punching tool N USOC PRO 002 AP05 Appendix 5 Issue 1 3 Meulding ef PVA Membrane Date 23 05 2007 Page 53 of 56 b orostlicate Cuttin 5 Remove the Outer Hole Punch by sliding it upwards while holding the Guide Sleeve 6 Confirm that the punched piece of Borosilicate Fibre remains fixed to the punching tool N USOC PRO 002 AP05 Appendix 5 Issue 1 3 Date 23 05 2007 Page 54 of 56 Borosilicate Cuttin The state after performing step 8 The piece of Borosilicate Fibre is clamping the PolyVinylAlcohol membrane which immobilizes the seeds 7 Move the Punching tool to the Growth Pot and pl
43. run approximately 15 were broken in addition to the main experiment objective It is desirable to cultivate wild type and mutant strains of Arabidopsis thaliana under microgravity and at 1 g conditions on orbit Table 4 2 summarizes all CW RW Experiment Requirements which were changed or broken as a consequence of the anomalies mentioned in this section Appendix 6 gives a detailed overview of all the executed changes during the CW RW Experiment Table 4 2 CW RW Experiment Run Requirements not met due to EUE or EMCS issues and further changed upon PI ESA request Requirement ADO1 As in the IRD Altered Reasoning Originator Hydration APO3 1 CWRW EIR Exec 04 2 CWRW EIR Exec 05 3 CWRW EIR Exec 06 1 Initial hydration shall provide each PCC with 13 1 ml water 2 The EMCS EC position EC PCC EC Position A1 lefty EC Position A2 hmg EC Position B1 efty EC Position B2 hmg will be watered at experiment run day 1 3 The EMCS EC Position A3 Wild EC Position A4 GM Wild EC Position B3 Wild EC Position B4 GM wild shall be watered on the experiment run day 4 1 There is no method to determine the actual water content in the growth pot 2 amp 3 The hydration problem during the CW RW experiment led to several initial hydration attempts in Several positions and on various days Appendix 5 Anomaly PAR HW 001 1 Payload Anomalies PI ESA Power interruptions CWRW EIR Exec 31 CW RW desir
44. the lights in position B4 were switched to FULL from REDUCED on day 20 after initial hydration Rapid observation of EC B4 was also implemented every 5 minutes In the following week images of B4 and sensory data indicated that water was needed Several failed attempts were made to hydrate this position and reverse pumping was also attempted Reverse pumping is to suck water from the EC and pump it to the dehumidifier to remove any blocking particles or debris in the needles The light in B4 was also switched from full to reduced intensity and the temperature in the EMCS incubator was lowered to 20 C to avoid dehydration Unfortunately there was no indication that B4 received water Figure 3 6 shows EC B4 on May 15 looking healthy and the condition of B4 the week after Due to the watering inability JAXA decided to request an early harvest operation Doc Nr N USOC REQ 025 EMCS CW RW Requirements Integration Issue 1 R T 28 11 N USOE x eport Date 28 11 08 Norwegian User Support and Operations Centre Page 20 of 30 Samfunnsforskning AS Figure 3 6 Images of EC B4 taken on May 15 and on May 22 after continued watering attempts On May 23 commands were sent to prepare the facility for harvest Garrett Resimann stopped the CW RW Experiment and initiated the harvest procedure The CW RW Experiment was concluded by harvest of plants in B3 to 1 KFT with RNALater and B4 largest plants to 1 KFT with RNALater small plant to
45. undamaged The o ring at water nipple is properly installed and undamaged wet Fig 8 Trouble Shooting f r Dok Nr No EMCS RP 0000 151 DOR EADS S P R 283 Ausgabe Issue 1 Datum Date 18 8 2008 SES rm Seite Page 16 von of 24 5 removal of donuts Fig 6 Fig 5 Fig 6 6 removal of borosilicate layer Fig 6 7 The PDA membranes are completely dissolved Fig 7 Fig 8 7 removal of PP felt Fig 9 10 Zeolithe touching the felt sticks on surface is a bit humid as well as the borosilicate sl Dok Nr No EMCS RP 0000 151 DOR Trouble Shooting for EADS Ausgabe lssue 1 Datum Date 18 8 2008 ei Es Lr iL rm SPR 283 Seite Page 17 von of 24 Fig 9 Fig 10 8 emptying and collection of growth pot zeolithe granulate Fig 4 11 Zeolithe is removed and collected carefully with a spatula Beside some brownish grains there are also dark black coloured ones visible Where the zeolithe is humid it sticks together Top 5 mm are loose but can easily be separated Sticking increases radially from the center towards the wall After emptying the PCC some zeolithe still sticks on the air inlet holes Fig 11 The sponge is wet and bacl particles are visible Fig 12 Fig 11 Fig 12 9 removal of bottom chamber lid Fig 13 14 The bottom chamber is completely dry The borosilicate layers incl mesh are pro
46. uo pri D9d aveg OR av youeq MOY ey episjino peuuoued eq ueg 001 Dugem pr A jo esn Aq Pit DDd WOY SpIT rur sAOWsY PIT JIJ u pajesbajui JI ueiqtu uu SJEII ISOJOG YUM PIT 3od ujmoi9 jo uonejB lul E L Z uonduosag dee OT p Siqe 1 95 Jo 9 abey 200z S0 ez ee CL anss eunpeoo4gd df 3es peas pue Ajquiassy 99q L ueBn niw Bt Z00 Oud D2OSf N g N eouewnssy ent UonevoT aleq A0 psw ohad Lois roh S gd So lot 0 0 hh m Z 3 ALALATATALALALN pauuoLag z das or MISYON TE 1E DE WAQ ONO WN Z 0 S96 NIG SXZIA SMeuos uno eui YM 31 Yoepe pue jog UMOJS uo uonisod Jedoid u pri 03d edeld song Buiqiosqe sayem sy AJU A 01 Uoneziua s Jaye yi uo Jejem JO Sjejdoup pews ae pue Allejlwis jo no ajeoyisosog jo eoeid e eall sejnuiu 09 Joj 9 044 Je ezijwe s Ap pue jio uintuluinje UNM 48409 jexeeq XaJAg ju OGZ ui PI 29d paeau Ijd youaq moj y eprsjno pouwoued eq UBD SPIT UIA ay JO suonisod ayy uojeui EYS sejou u G xipueddy ur pequosep se joo Buijoung ejyeoairsojog eui YM aejeaijisoJog y ui sejou Ind Yleway ojoyd owagq uonduosaq deis juo p eie L 95 Jo 3884 4002 S0 z awa 1 enss aJnpa201d dN I9S peas pue Alqwassy 3234 L uSByinn Deen per teg ZO0 Otid DOSN N souBinssy NO UOge20 ae Aq peuuouag PAs mp SCH 30 10 hc 50 ori ia MSOLO
47. will be about 1 day and the checkout at KSC 2 days N USOC PRO 005 H US0C mwan CW RW Flight Sample Hardware Packing Transport issue 2 B T E and Check Out Procedure Date 27 02 2008 Page 11 of 58 6 Procedure Description The procedure is divided in 3 parts described below The CW RW ECs and EUE has been pre treated according to procedures ADO2 and ADO3 as reported in ADO4 Packing Procedure at N USOC The CW RW ECs with integrated PCCs will be photographed and individually packed in zip lock bags provided by NASA at N USOC The ECs in ziplock bags will be installed in three aluminum transport boxes covered with foam 3 ECs are installed in box 1 and 2 S N FM092 FMO94 FMO95 in Box 1 and S N FMO96 FMO98 in Box 2 and two ECs are installed in box 3 S N FM099 FM100 A temperature logger is placed inside each box The boxes are stored at ambient temperature 22 C 3 C Transport Procedure from N USOC to KSC The CW RW ECs with samples will be transported in aluminum framed boxes with foam Transportation from N USOC to KSC will be achieved by car and airplane The boxes will be hand carried during the flights and the following requirements shall be obtained The boxes must be handled with care The boxes shall be transported at ambient temperature 22 C 3 C The boxes shall be transported at ambient pressure 1 atm Checkout Procedure at KSC If the ECs are not cleaned according to NASA requirement
48. 00 Fax 81 22 795 6669 The following information is submitted in support of shipment of seeds by Norwegian University of Science amp Technology NTNU OO n n E The name of supplier is Professor Kazuhiko NISHITANI Tohoku University Sendai JAPAN The name of contact person for supplier is Professor Kazuhiko NISHITANI The name of the seed is Arabidopsis thaliana The variation is Columbia Col O The sced stock was harvested December 20 2007 Production number is 2007 02 The seeds are untreated The seeds will be received by Motoshi KAMADA JAXA Regards A WA pasa Professor Kazuhiko NISHITANI Tohoku University Aobayama Aoba ku Sendai Japan 980 8578 Phone 81 22 795 6700 Fax 81 22 795 6669 e mail nishitan mail tains tohoku ac jp i N USOC PRO 005 N USDD mm CW RW Flight Sample Hardware Packing Transport issue 2 and Check Out Procedure Date 27 02 2008 Page 39 of 58 eg e Tohoku University cA ui Aobayama January 28 2008 te ni Aoba ku v D Sendai es 9 JAPAN 980 8578 TOHOKU Phone 81 22 795 6700 Fax 81 22 795 6669 The following information is submitted in support of shipment of seeds by Norwegian University of Science amp Technology NTNU The name of supplier is Professor Kazuhiko NISHITANI Tohoku University Sendai JAPAN The name of contact person for supplier is Professor Kazuhiko NISHITANI The name of the seed is Arabidopsis thaliana The variation is Col
49. 005 Cell Wall Resist Wall KFT MELFI Insert Harvest MELFI MGUEEXPRSCWRWN005 Insertion of EMCS Ref ECs Crew Proc Replace CW RW ECs with Ref ECs 2 005 EC Replacement MGUEEXPRSEMCSNO05 EMCS Dry Out Schedules Preparation for hibernating EMCS Crew Proc Closing of gas valves 2 024 EMCS Valve Movements for Ground Commanding MGUEEXPRSEMCSN024 Stowage at 95 C in MELFI before packing in Double cold bags for Ascent NASA responsible with N USOC Support On March 30 2008 Crew inserted the CW RW ECs in the EMCS positions as can be seen in table 3 5 Initial hydration of the EC A1 A2 and B1 B2 was attempted and progressed very slowly The hydration attempt only resulted in a small increase in rh for EC B1 After a couple days it was clear that hydration of the first four ECs was non functioning and potential causes were thought to be incorrect connections between EMCS RBLSS boxes EMCS Water Reservoirs and EMCS ECs Crew time was requested in order to verify correct configuration of EMCS Water Subsystem connections Astronaut Garrett Reisman performed the EMCS Water Flow Checks on April 5 2008 and reported that he could not see any obvious failures After the Water Flow Checks hydration of all ECs except EC B4 was attempted several times but showed little difference in PCC rh The hydration of EC A1 was not sufficient to trigger the delta pressure sensor which indicates a full growth pot it was ho
50. 0179 Quality Assurance Bjernar Vasenden Prototech 47 555 74103 PCC Cleaning N USOC TR 027 CW RW Issue 1 PCC Assembly and Seed Set Up Report Date 13 02 2008 Page 7 of 105 6 Set Up The set up was performed at the Plant BioCentre in Trondheim Norway 8 Flight ECs FM092 FM094 FM095 FM096 FM097 FM098 FM099 and FM100 and 8 PCCs were first cleaned by Prototech after being utilized in CW RW Biocompatibility test and CW RW Germination Hydration test before set up Arabidopsis Thaliana seeds Wild type GM GUS lefty and hmg used for set up was supplied by JAXA Science team and sterilized at N USOC by Set up Engineer Motoshi Kamada The Zeolite used for the set up was from the same batch as prepared for the Multigen 1 experiment and no new enrichment or filtration of Zeolite was performed during set up of the CW RW experiment Motoshi Kamada performed CW RW growth pot sterilization growth support filling seed integration implementation of immobilization membranes borosilicate doughnuts and mini lids as described in ADO4 and AD05 Assembly of the PCC and implementation of EC cover was performed by H kon Svare The preparations and set up are documented in the filled in procedures Multigen 1 PCC Assembly and Seed Set up Procedure in Appendix 1 and CW RW Seed Set Up Procedure in Appendix 2 Pictures illustrating parts of the Set Up can be found in Appendix 3 All changes and variations during set up w
51. 1 1 14 05 07 Added steps for weighing Zeolite 12 14 05 07 12 13 Ja Additional changes made to j 1 2 14 05 07 procedure in Appendix 1 N USOC PRO 002 Multigen 1 Issue 1 3 PCC Assembly and Seed Set Date 20 06 2007 Up Procedure Be Log of Changes since Base lining Reason for change Issue Date Page s Step s Corrected reference to appendix 20 06 07 Step 21 Amount of MS Medium corrected 1 3 20 06 07 2 Step 6 Relocated step of dust particle removal to after 1 3 20 06 07 11 Step 1 4 enrichment Changed method of removing dust particles Labeling of growth pots and Lids during the entire 20 06 07 EJ set up Growth pot shall remain in sterile bench over night to 20 06 07 be dried and not be transferred to exsiccator and Weighing of Growth Pot not Zeolite 13 200607 13 14 Step 11 14 heating cabinet 1 3 06 k Changed method of packing the Zeolite the growih 1 3 20 06 07 13 Step 12 pot shall not be tapped against the surface of the work bench but rather be tapped with a blunt object Attaching the borosilicate paper in the recession of 1 3 20 06 07 16 Step 19 the Growth pot lid need not be performed in the sterile flow bench The cardboard for cutting the borosilicate for the 1 3 20 06 07 Step 20 Growth pot lid need not be sterile 1 3 06 7 3 until Zeolite is compressed Specialized mini funnel is used to gui
52. 2 CW RW Flight Sample Hardware Packing Transport and Check Out Procedure Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 Integration Report Date 28 11 08 N USOC ko Page 8 of 30 Samfunnsforskning AS Norwegian User Support and Operations Centre 1 Scope 1 1 Purpose This document describes the results and a validation of the CW RW Experiment Requirements Integration Process performed by N USOC The integration was based on the Experiment Requirements documented in the CW RW Integration Requirements Document ADO1 as well as Test Plans Appendix 1 which identified requirements that had to be tested by the Pl team at N USOC in the EMCS Ground Models either to verify that the requirements could be met or to optimize integration into the EMCS facility 1 2 Document Overview The scope of the document is summarized in section 1 the CW RW experiment scientific objective and experiment flow are described in Section 2 Section 3 contains a summary and a validation of how the Experiment Requirements were integrated pre experiment 3 1 3 2 amp 3 3 during the experiment 3 4 and post experiment 3 5 Section 4 identifies Payload Anomalies and incidents which led to alteration of Experiment Requirements Section 5 summarizes the conclusion of the integration 2 Introduction 2 1 Scientific Objective Cell wall Cell walls of plants play a crucial role in forming supporting tissue in ste
53. 23 Houston TX 77058 FAX 281 483 3058 TO Mr Wilfried Biemann Astrium GmbH TOQ5 49 7545 8 4225 FROM Dr Shannon Langford U S Toxicologist 25 Progress Voice 281 483 2137 Pager 877 305 0566 DATE 25 Jan 2008 SUBJECT Verification Step 2 V 2 Form for As Loaded Materials listed in the Hazardous Materials Summary Table HMST PSRP Policy The JSC Payload Safety Review Panel silows experiment containers to be loaded only with materials in amounts and concentrations less than er equal to those listed in the final HMST for that container or with materials in the same HMST approved for other containers of the same experiment The levels of containment of the materials must bo consistent with the Hazard Level assigned Instructions Ihe V 2 form is to be completed at the time that thy materials are loaded into their flight hardware or as soon as feasible thereafler Please mark up the verified V i HMST noting ali doletians reduced amounts or concentrations changes in location in the experiment system or any other allowed changes The mark ups to the HMST should accurately telicct the materials that ate actually loaded The responsible party will sign the V 2 form and check the appropriate box 10 indicate whether corrections hava been made lo the HMST Then e mail a scanned version preferred of this form ard the HMST pages with corrections to Shannon d langford lt nasa gov If the HMST does not require any mark ups only the sig
54. 23 Repeat step 6 8 pulse ok 24 At syringe read out total volume and collected water volume total 10 8 water 9 7 25 Syringe was emptied start at zero ok 26 Repeat step 6 9 pulse ok 27 At syringe read out total volume and collected water volume total 1 3 water 1 2 28 Repeat step 6 10 pulse 29 At syringe read out total volume and collected water volume total 2 6 water 2 5 30 gt at GSE RH ctrl switched to 50 source DH ok 31 Repeat step 6 11 pulse 32 At syringe read out total volume and collected water volume total 4 2 water 4 1 33 Repeat step 6 12 pulse 34 At syringe read out total volume and collected water volume total 5 5 water 5 3 air bubble from RH loop passes the filter 35 Repeat step 6 13 pulse 36 At syringe read out total volume and collected water volume total 7 1 water 6 8 37 Dismount and weigh EC 1146 7 gram 7 4 gram added gt 7 4 6 8 0 6 gram in filter 38 Weigh Water Reservoir as reference 1263 8 gr gt 16 9 gr removed from fresh EADS Sei cbriL i rnm Trouble Shooting for SPR 283 Dok Nr No Ausgabe lssue Seite Page 1 9 Datum Date von of EMCS RP 0000 151 DOR 18 8 2008 24 300 4 250 End to end test with empty EC no PCC Water Reservoir FM009 RBLSS FM008 Rotor I F adapter SN10 EC FM092 pressure mbar m content of syringe ml water 4 content of syringe ml air PW1
55. 26 0 C 24 0 C 22 0 C 20 0 C 18 0 C 16 0 C 14 0 C 1 mar 08 2 mar 08 3 mar 08 4 mar 08 5 mar 08 6 mar 08 00 00 00 00 00 00 00 00 00 00 00 00 CWRW Box3 EC099 EC100 Page 1 of 1 SL52T Standard print printed on fredag 16 mai 2008 at 14 01 27 Logger Serial No C8000000050B6041 Lo Alarm Setpoint 40 0 C Total readings since new 25343 Hi Alarm Setpoint 85 0 C Sample Rate 10 mins Alarm s Triggered No Logged readings 4096 Calibration due Not Set Issue Time Date 29 feb 2008 9 59 33 AM Graph Start 29 feb 2008 11 38 00 PM Graph Stop 6 mar 2008 7 00 00 PM Owner Manifest Not Sel User Manifest 1 CWRW Box3 EC099 EC100 User Manifest 2 Logger 5 Signature 1 Signature 2 Print Name Print Name Date Date i i H H n I ppp li i MAX 31 86 C MIN 14 29 C RANGE 17 57 C mE MEAN 18 83 C 30 0 C 28 0 C 20 0 C ee i l SR BE UN Ei e it 16 0 C a BU 14 0 C Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 Integration Report Date 28 11 08 N USOG m3 4 S Se Norwegian User Support and Operations Centre Page 1 of 1 Samfunnsforskning AS Appendix 5 CWRW Hydration Summary 92 30 62 pulses Resat __ counter to 0 Pump running continuously for 2 min Power down for crew activity EMCS Water Flow Checks Counter set to 25 to avoid rapid pulsing _ Hydration performed in JAOS only Sch stopped
56. 3g L ueBniniy men PSE voneAuSu 00 OHd DOSIrN eouginssy Ayjenp uoge201 sed A0 pouvouay PAS vba S gd gO lo AT sgo 102 vpn mu 590 anssy Jaded say jsnp auas pue di D e ui euoaris sseoxe yo edi DI 39d eui Jo eoseuns SY uj pejjere HUN SPIT Ou 84 ur M8JDS pue joo L Buiuoeny pr UIA out esp spealuj PI UN ay punoje peejds AjueAe ejnueo poys uui CL e UMA eBuu s e ou Dot eq paoueApe ur pinous ATA TA LATATA ATA euooyis ay lL D BI auodyis UPM pessy afew pr rug au Ajddy Og PIT 990g eui jo aseuns ay eAoqe WW noge aq eys SPI Wal eui JO abpa Jeddn y joo Buyseny pri u au UM SPI iuny ay meuos Bujyoeye sayy pri eur out p ju uu jdur Huq aiojaq OUELIS Bunung Aq peziue s eq NEYS spr ILN G xipueddy ui einpeooud op Guploooe spr muy pue jnuuBnop ayeoyisoiog eu Junoyy 6c CHONO ejeoi isoJog sy uo aem jo sjajdoup Buippe Aq ayeoyisolog jo senijigedeo Gulquosqe 1ajem 0840 g xipueddy 001 Buruopung ejeoiiso10g ay Guisn Aq ejeoyisojsoq peziuers Kup jo JNUYBNOP jews e oyeyy gz 1 3D EAA eseeic uinnoeA YBIH auos esesJe euooili uo2l 1S YNM Burnoas pue Spr Iur yo UONEJBaIU 97 L4 pauuopad uoy ojoug ouag uonduoseg dajs days WOD p aqe 95 Jo 02 eBed 400z 80 Z a1eq CL enssi Sinpe20oid AN I9S pass pue Ajquiessy 99d p uabninin uin pit m
57. 40 gE abed 100z S0 ez eg Jod YWMOID 99d uo Buods jo uogexiJ CL anssi E xipueddy 1182418949 Ser ganesus j 00 Otd OOSfT N eougunssy y enp uoge2o sea A0 pauuojsg bip Peso ae E VW 716 SIL gO JOEZ Pre RO O h FIDA Ku 09 L aigeL 99 10 LE beq 100Z S0 z aea Jod YWMOID JIJ uo abuods jo uogexiJ CL anss xipueddy HOESER 00 OHd O2OS t N eougunssy Ayjenty uOHE201 jeg A0 pauuopad 5 3 TA Cc p d A N d 3 PA gt Pli SM 80 JOE PYD 3010 Hl EG MOE OSI JM HA alg pred VO bO PUO Shows SDF s k mm cocAQ VEAL AOU 200 yip 0 PHO BbLO lLD ALO 62 TLOW4 522 Of sabuods pyvmnou Pran 4 20101209 Pen rauvnlg ory anjeA jenjoy souBlajo r anjeA jeumuon uonduosaq dajs j59 on days isa e1np9204d euj OF suoneueA pue sabueyy z Sigel G ejqej ui peyueuunoop eq EYS eunpe2oJg eui 0 suoyenea pue seBueuo y Ul 13 UONEMEA e1npao20Jg 1591 Z 9G Jo ge abed 1007 60 ez om Jod YIMOUS 99d uo eBuods jo uonexiJ CL anss c xipueddy Dsg 00 08d 20SN N N USOC PRO 002 AP03 Appendix 3 Issue 1 3 Fixation of Sponge on PCC Date 23 05 2007 Growth Pot Paga 39 of 56 3 Procedure Sign off All steps and activities described in the Procedure have been successfully performed Failures and non conformities raised during procedure execution were listed in table 2 Procedure sign off Engineer Date m THOIS Emoag OG T H LEZ Sork ez E
58. 46 833 2181 7 7 8 778 1 1 47 847 2173 7 8 9 781 1 2 48 855 2143 7 8 10 806 1 2 49 866 2152 7 8 11 837 1 5 50 880 2153 7 9 12 855 1 7 51 885 2121 8 1 13 842 1 9 52 893 2117 8 3 14 833 2 1 53 893 2117 8 5 15 827 2 3 54 916 2108 8 7 16 829 2 5 55 922 2095 8 9 17 826 2 7 56 1051 2093 9 1 18 819 2 9 57 1300 2113 9 1 19 823 3 1 after 5 minutes 20 818 3 3 1500 gt 2000 delta weight increasing real value 11 9 g 21 819 3 5 gt 0 1625 ml pulse 22 824 3 7 23 819 3 9 24 815 4 1 25 819 4 3 26 819 4 5 RH ctrl 50 4 minutes on only RBLSS to generate air bubble delta t 5 min 27 809 4 7 28 811 4 9 29 809 5 1 30 796 5 3 31 800 5 4 32 790 5 4 Dok Nr No EMCS RP 0000 151 DOR Trouble Shooting for EADS me SPR 283 Ausgabe Issue 1 Datum Date 18 8 2008 E r ILA mMm Seite Page 12 von of 24 Pulse pressure estimated water Pulse pressure estimated water analog 2 mV volume in EC analog 2 volume in EC 33 803 5 4 34 806 5 4 35 806 5 5 36 814 5 7 37 818 5 9 38 810 6 1 39 796 6 3 40 818 6 5 interrupted for today End to end test with PCC Water Reservoir FM009 RBLSS FMOO08 Rotor I F adapter SN10 EC FM099 2500 20 2300 18 p PCC analog 2 mV water volume in EC ml 2100 16 1900 14 z m 1700 12 8 Q 9 o 2 1500 10 amp trigger
59. 8 Page 56 of 58 Description of containment and transport for Cell Wall Resist Wall experiment Material Usage The 104 Arabidopsis thaliana var Columbia Col O seeds which includes 30 wild type WT 14 gene modified GM GUS 15 lefty mutants and 15 hmg mutants will be used in an experiment named EMCS Cell Wall Resist Wall This experiment is an ESA European Space Agency and JAXA Japan Aerospace Exploration Agency sponsored experiment to be performed in the European Columbus lab on board the International Space Station ISS under an ESA NASA agreement The experiment is planned to fly on Space Shuttle 1 J A planned for a 2008 March launch The final handover at KSC Florida is planned for March 2008 Containment The entire collection of 104 Arabidopsis thaliana var Columbia Col 0 seeds which includes 30 wild type WT 14 gene modified GM GUS 15 lefty mutants and 15 hmg mutants will at ALL TIME including germination phase be contained within a minimum of one layer of containment For the transport to inside US to the ISS the seeds will be within Experiment Containers see figure 1 providing one layer of containment After germination of seeds inside the Experiment Containers the plant material will be removed from the enclosed Experiment Container inside a Microgravity Science Glovebox onboard the ISS and put into Kennedy Fixation Tubes see figure 2 where the plant material will be kept within one layer of containmen
60. 9 01 81 NO 7481 Trondheim Dragvoll Alle 38 Fax 47 73 59 01 77 Org No NO 974 767 880 N USOC PRO 005 H USOE ee CW RW Flight Sample Hardware Packing Transport issue 2 emm and Check Out Procedure Date 27 02 2008 Page 54 of 58 H USOG Rorweptta Ure Sapsort ant Oserations Contre EAN OPY YES NTNU Social Research Ltd The cargo is transported in three aluminum frame boxes see example image below for model type covered with foam on the inside Transpor box 1 and 2 contain three Experiment Containers ECs each FM092 FMO94 FMO95 ard FMO96 FMO97 while Transport Box 3 contains two ECs FM099 FM100 Transport box 1 Inside FMO92 there are a total of 7 Arabidopsis thaliana Wild Type seeds placed in 7 holes in the lids of the Plant Cultivation Chambers PCCs Inside FM094 there are a total of 7 Arabidopsis thaliana GM GUS seeds placed in 7 holes in the lids of the Plant Cultivation Chambers PCCs Inside FM095 there are a total of 4 Arabidopsis thaliana Wild Type seeds and 15 Arabidopsis CEN mutant lefty seeds placed in 7 holes in the lids of the Plant Cultivation Chambers PCCs Transport box 2 Inside FMO96 there are a total of 4 Arabidopsis thaliana Wild Type seeds and 15 Arabidopsis thaliana mutant hmg seeds placed in 7 holes in the lids of the Plant Cultivation Chambers PCCs Inside FMO97 there are a total of 7 Arabidopsis thaliana Wild Type seeds placed in 7 holes in the lids of th
61. 9 ke Figure 3 4 Image of EC B1 after the final watering attempt was performed on May g Doc Nr N USOC REQ 025 EMCS CW RW Requirements Integration Issue 1 Report Date 28 11 08 Page 19 of 30 On April 30 and May 4 although strongly advised against by N USOC ECs B3 and B4 were added 5 pulses of water on request from PI team A delta pressure check in ECs B3 and B4 was also requested by Pl team to see if the seedlings needed water again on May 7th It was advised against a delta pressure check by N USOC because of the danger of flooding the bottom chamber The delta pressure check was performed anyway and when performing this check 15 seconds in Watering Mode the dP triggered in both ECs indicating full growth pots The check lead to water flooding the bottom chamber in EC B3 PAR EMCS HW 013 and the flow stopped Attempts were made several times to start the humidity control in B3 but this failed Due to no flow in this EC the lights were switched off to avoid an increase in EC temperature Lights remained on in the other ECs After five days and continued attempts the humidity control in B3 was started successfully This was however too late for the seedlings in B3 and they had wilted due too drowning see figure 3 5 F mn N KEN D ea D TW Figure 3 5 Images of EC B3 taken on day of flooding the bottom chamber left and five days later when flow was resumed in this position right On May 13
62. 91 Trondheim CW RW PCC Assembly and Seed Set Up Report N USOC TR 027 Issue 1 Date 13 02 2008 Page 2 of 105 Table of Contents 1 Documents ee ns an ne 3 1 1 Applicable Documents u aan aus qs 3 1 2 Reference Documents 3 2 List of Abbreviations scssi a R EEEE ETNEN 4 3 SUmmaryY EE 5 4 Vth OU Ch OM vs iu iet RE eire e EE nen ERE Rer ER EIER 5 S KE TEE 6 EE 7 7 JdNonscontorimities oec eee tete tee etes tocca pee EE 8 NEM RESUItS koton aaa dbide ones r E E ansat Vosierdeenean Gevveddsedee te 9 O Conc e s NE 9 Appendix 1 Filled in N USOC PRO 002 Multigen 1 PCC Assembly and Seed Set up See TEE 10 Appendix 2 Filled in N USOC PRO 004 CW RW Seed Set Up Procedure 86 Appendix 3 Pictures from CW RW PCC Assembly and Seed Set Up 103 CW RW N USOC TR 027 Issue 1 PCC Assembly and Seed Set Up Report Date 13 02 2008 Page 3 of 105 Change Record Issue Date Page s Description of change Release 1 Documents 1 1 Applicable Documents AD Document Number Date Issue Title ADO1 EMCS MA 4000 002 DCR 12 11 04 2 EC User Handbook AD02 CWRW ISR 16 02 07 0 EMCS Cell Wall amp Resist Wall INTEGRATED SCIENCE REQUIREMENTS DOCUMENT AD03 N USOC REQ 007 06 07 07 1 EMCS CW RW Cell Wall Resist Wall
63. A2 Flight EC A4 Flight EC B1 Flight EC B3 Flight EC B2 Flight EC B4 EC FM 92 94 95 96 97 98 99 100 PCC FM 21 18 19 20 16 22 23 17 mini lid strain num strain num strain num strain num strain num strain num strain num strain num 1 WT 2 WT 1 WT 2 GUS 1 WT 2 WT 1 WT 2 GUS 1 2 WT 2 WT 1 WT 2 GUS 1 WT 2 WT 1 WT 2 GUS 1 3 lefty 3 WT 1 hmg 3 GUS 1 lefty 3 WT 1 hmg 3 GUS 1 4 lefty 3 WT 1 hmg 3 GUS 1 lefty 3 WT 1 hmg 3 GUS 1 5 lefty 3 WT 1 hmg 3 GUS 1 lefty 3 WT 1 hmg 3 GUS 1 6 lefty 3 WT 1 hmg 3 GUS 1 lefty 3 WT 1 hmg 3 GUS 1 7 lefty 3 WT 1 hmg 3 GUS 1 lefty 3 WT 1 hmg 3 GUS 1 Doc Nr N USOC REQ 025 EMCS CW RW Requirements Integration Issue 1 5 Report Date 28 11 08 Norwegian User Support and Operations Centre Page 15 of 30 Samfunnsforskning AS The preparations are documented in the filled in procedure PCC Assembly and Seed Set Up Report Appendix 2 Figure 3 1 A D illustrates parts of the Set Up Noe Figure 3 1 Images from EUE Set up at N USOC After Set up and Assembly of the PCCs the ECs were subjected to a final verification by the Industrial Operator Team IOT The ECs were checked for leakage and were balanced and adjusted for centre of gravity They were also checked for sharp edges by The Human Factors Implementation Team HFIT and the labeling was inspected by the Payload Label Approval Team IPLAT These processes that were performed by
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65. Assembly and Seed Set Up Procedure Issue 1 3 Als per formed Gang Heg lit preparations 14 01 07 34 01 07 N USOC PRO 002 Multigen 1 Issue 1 3 PCC Assembly and Seed Set Date 20 06 2007 Up Procedure Page Lente Multigen 1 PCC Assembly and Seed Set Up Procedure Prepared by Carina Helle Berg Reference Issue 1 3 Date of Issue 20 06 2007 Document type Procedure N USOC PRO 002 Multigen 1 Issue 1 2 PCC Assembly and Seed Set Bate 14 05 2007 Page 2 of 56 Up Procedure Approval Title Issue Multigen 1 PCC 1 2 Assembly and Seed Sei Up Procedure Author Signature Affiliation Date Suzanne veriie N USOC Wks 5 Checked by Sianature Affiliation Date Ann iren Kittang m N USOC ibj An Jen 9 7O i Knut Fossum l w p X Sege N USOC PM WE 3 Ulrich Kuebler ESA EMCS PIM Approved by Signature Affiliation Date Wilfried Biemann Astrium lOT PA Claude Brillouet ZAZA Je OF ESA EUE PM Tor Henning Iversen Ee Wnai A S SE Multigen 1 PI n Is _ DE Change Log Initial release 29 03 07 1 I Added check of Borosilicate water 14 05 07 16 19 Steps 20 26 absorbing capabilities Minor changes in procedure for 14 05 07 Step 1 2 Zeolite nutrition enrichment Deleted step in procedure for Zeolite 14 05 07 en s nutrition enrichment Changed agent for securing mini 1 1 14 05 07 Paragraph lids to PCC Lid 7 1 6 Updated version of App 2added
66. Centre Page 1 of 1 Samfunnsforskning AS Appendix 3 Flight Sample H W Packing Transport and Checkout Procedure N USOC PRO 005 N USOG CW RW Flight Sample Hardware Packing Transport issue 2 arwina Bror Logger und Bocrations Centre and Check Out Procedure Date 27 02 2008 Page 1 of 58 CW RW Flight Sample Hardware Packing Transport and Check Out Procedure Prepared by Maja Rostad Reference Issue 2 Date of Issue 27 02 2008 Status Document type Procedure Distribution As described in Approval list page 2 CIRIS Center for Interdisciplinary Research in Space NTNU Samfunnsforskning Dragvoll Alle 38 N 7491 Trondheim N USOC PRO 005 mmm mmm CW RW Flight Sample Hardware Packing Transport issue 2 PALY Rerweyize Diet Lesgar und aeren Centre yan and Check Out Procedure Date 27 02 2008 Page 2 of 58 Approval Title Issue CW RW Flight Sample Hardware Packing Transport and 2 Check Out Procedure Author Signature Affiliation Date Maja Rostad N USOC Checked by Signature Affiliation Date Ann lren Kittang N USOC Knut Fossum N USOC Manager Toru Shimazu JSF PI Rep Ulrich Kuebler ESA EMCS CW RW PIM Claude Brillouet ESA PM Added letter of IOT insurance coverage Import permit V2 form and NASA Cover Letter N USOC PRO 005 mmm CW RW Flight Sample Hardware Packing Transport issue 2 E ni am and Check Out Procedure Date 27 02 2008 Page 3 of 58 1 2 3
67. Conditions during Experiment Get Up eene 9 7 Pfoced re EE 11 TV Assembly OF POG u aeterne discere nemen estes 11 7 1 1 Assembly of Growth Pot with Sponge and Zeolite 11 7 1 2 Implementation of PP Felt on top of Zeolite 14 7 1 3 Integration of Growth Pot Lid with borosilicate membrane 16 7 1 4 Integration of Arabidopsis seeds eene 18 7 1 5 Integration of immobilization membrane PVA and borosilicate doughnuts 19 7 1 6 Integration of Mini Lids and securing with Silicone 20 7 1 7 Assembly of POG wii u u u tive ete konsa P epa dont eate ea s zaekosayozoge Site 21 8 Test Procedure Variation Fill in sssesvrrnrrenresrannronnensvrsnresnneenrennesnvnsnresvnsnnssnnrensennvennn 24 9 Procedure EE 26 Appendix 1 Zeolite Nutrition Enchment eese 27 Appendix 2 Procedure for filtering of Zeolite sees 32 Appendix 3 Fixation of Sponge on PCC Growth Pot 33 Appendix 4 Moulding and sterilization of PVA membrane a sssssesssessssrirssrrsrrrsrsrerarssn 40 Appendix 5 Borosilicate Cutting 49 N USOC PRO 002 Multigen 1 Issue 1 3 PCC Assembly and Seed Set Pate 20 06 2007 Page 5 of 56 Up Procedure Abbreviations EMCS European Modular Cultivation Chamber ERM EMCS Reference Model EC Experiment Container PCC
68. Crew Procedures CW RW KFT MELFI Retrieval CW RW MSG Set UP CW RW Harvest in MSG etc Doc Nr N USOC REQ 025 EMCS CW RW Requirements Integration Report Issue 1 N USOC Date 28 11 08 Norwegian User Support and Operations Centre Page 10 of 30 Samfunnsforskning AS Pre flight 10 50 Post flight L 40 L 20 L 10 Cultivation from seed to approx 10 Preparations at N USOC Lab Post flight assessment of samples at PI Lab Transport to KSC KSC Preparations and Checkout Transport toP Lab KSC stowage Stowage in MELFI pending launch KFT Retrieval by PI Download Upload 8 ECs KFTs Activity Crew bh Description Interaction Figure 2 2 CW RW Pre flight on orbit and retrieval timeline as planned before experiment start Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 Integration Report Date 28 11 08 S Page 11 of 30 Samfunnsforskning AS Norwegian User Support and Operations Centre 3 The Experiment Requirement Integration Process as Performed The CW RW Experiment s Integration Requirements are documented in the CW RW Integration Requirements Document N USOC REQ 007 AD01 The current document summarizes the actual products developed during the Integration phase and describes in which way different requirements were met on orbit The CW RW Integration and Real Time Operations Flow with time aspects responsible parties and resulting products can be seen in tab
69. EMCS HW 0013 and the Automatic subsystems shutdown due to lost communication between the SPLC and the TCS EMCS SW 0007 and EMCS SW 0009 In addition there were a lot of incidents where the EMCS SPLC lost communication with the Express Rack 3 Rack Interface Computer RIC PAR EMCS SW 0001 This was seen in form of Health amp Status telemetry dropping out and in again for different intervals ranging from 1 2 seconds to several minutes Table 4 1 lists the PARs that were reopened or established during CW RW PAR Description of PAR and Impact on science and CW RW Requirements ADO1 Affected Requirement Corrective act and Work arounds during CW RW Impact on CW RW after implemented workarounds Additional requirement affected after work around EMCS SW 0001 Loss of EMCS HK H amp S Packages Loss of images and other TM data for minutes several hours on several occurrences Obs target and freq EIR Exec 09 1 Power cycle EMCS SPLC 2 Reduction in EMCS TM Packages for a period until the TM had stabilized 1 Resumed environmental control and images Environmental control not effected Gravity level EIR Exec 13 Illumination EIR Exec 15 Power interruptions EIR Exec 31 Obs target and freq EIR Exec 09 EMCS SW 0002 EMCS SPLC Subsystems Loss of Com Loss of environmental control and images for 1 2 hours One occurrence Unwanted triggering of the EMCS H W safety circuit resulting
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75. LTIGEN these ECs were mounted in the following rotor positions Rotor A A1 A2 A3 A4 EC FM055 FM056 RBLSS Module FMOO1 FMO006 Rotor B B1 B2 B3 BA EC FMO58 FM057 RBLSS Module FM005 FMOO4 Observations of MULTIGEN ECs returned ECs are highlighted EADS Dok Nr No EMCS RP 0000 151 DOR Trouble Shooting for SPR 283 Ausgabe lssue 1 Datum Date 18 8 2008 E r rum Seite Page 24 von of 24 EC S N Rotor PCC S N PCC holes Main off nominals Position FM052 A1 FM04 3 germination sacrificed as water reservoir to drain the waste FM055 A2 FM02 3 plants partly no flow FM053 A3 FMO5 3 germination dried out FMO56 A4 FM10 5 partly no flow germination dried out FM054 B1 FMO8 5 germination no venting possible dig line failure plants dried out FM058 B2 FM09 3 triggered after 200 pulses germination dried out FM057 B3 FM11 5 no germination FM059 B4 FM12 5 germination dried out EC FMO55 to FM057 were opened from the bottom the filter side for inspection and found to be in proper condition with filters still wet about 9 months after the end of MULTIGEN 1 processing in EMCS The PCCs were not dismounted This inspections gave no clue on the insufficient performance during the MULTIGEN 1 experiment in EMCS EC FMO58 was not opened and used as returned to ground for a hydration test in the same set up as described in chapter 4 3 as a pr
76. N RFRFEERRER 6 Table 3 1 Participantfs SIGE Seats suene ne dd One E pate snek re iem OR UTR DN P NR SAL 7 Table 4 1 Equipment List Kuss SEENEN 8 The Information contained herein is proprietary to PROTOTECH AS and Is to be used by the recipient solely for the purpose of which It was supplied It shall not be disclosed in whole or in part by any other party without the written permission of PROTOTECH AS SV H2310 LOtid JO UDISS LJSO uem ayi JNOYM Aed Jayjo Aue Aq ped uj JO ajoum U pasopsip sq Jou jeys 31 payddns sea 3i u juw JO asodund ayi Joj Ajajos auajdpeu ayi Ag pasn sq 09 si pue sy HJYLOLOUd 03 Asejajidoud si wasay paujeyuon uopeuiojul SUL uondiiosag dags 133HS NOLLVIAIG 9YNAII0Ud W XTANIJdav er 40 LT sabe V XTONSAddv ESTOE Valo L00Z S0 E0 93eg a anss yjo z JO GuwSYNI 104 BIANPBIOJJ SO Ud E6TOE ud ou 20g 201 sjueuiLiodxa SOWA Oud TO LINW ud XAW3 ou a0q Doc no EMEX PR MULT 04 PRO prototec h ze EMCS Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 APPENDIX B Page 18 APPENDIX B NONCONFORMANCE LIST NCR DESCRIPTION The information contained herein is proprietary to PROTOTECH AS and is to be used by the recipient solely for the purpose of which it was supplied It shall not be disclosed in whole or in part by any other party without the written permission of
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78. ON nm e eo pressure at EC inlet mbar a eo E e eo EC turned top syringe emptied o content of syringe ml 50 0 4 08 20 00 08 30 00 08 40 00 a 08 50 00 time 09 00 00 farm Figure 4 6 Result of water supply test without PCC 4 3 2 End to End Test with EC FM099 09 10 00 0 09 20 00 Setup according to Figure 4 7 EC FMO99 is in original flight configuration with PCC mounted orientation EC horizontal top side down Since the water tubes are still filled from the previous test no initial filling is required The Water Reservoir is oriented with fresh water bag upside keeps air bubble inside bag Since the RBLSS delivers a controlled flow of approx 400 ml min but the PCC requires 200 ml min the EC is by passed and the flow to EC measured with an external flow meter Water Reservoir flow adjustment EC Figure 4 7 Setup for combined test with EC099 with PCC A Dok Nr No EMCS RP 0000 151 DOR Trouble Shooting for EADS SPR 283 Ausgabe lssue 1 Datum Date 18 8 2008 E r 1L Seite Page 10 von of 24 Test Water Reservoir RBLSS Module Rotor UE EC Note outlet outlet Adapter water service FM009 QD10 FM008 B2 QD04 S N 10 FM099 with PCC path 6 Test Equipment Rotor I F adapter S N 10 adjusted to worst case tolerances low interface high rails pressure sensor tube kit RBLSS GSE OHB
79. ONEIP Y wiousd L0 MH HVd 62LLND 6ZL LWS 62LLND dey Id abeys siu je uo Ajsnonunuoo eg ut SJYBI uo youms pajuawajduy sjue d ey JO jueyodui Jon si uoneuruunj se USYE ag eys sjexood jesy pedojeAep jo deu au WEaj la ay UIYNM SUOISSNISIP Jayy 10 MH HVd BZLLND STZLLND 8cLLWO day Id O3 SIY ui Moe ou BABY am eouis yo payojims Sy g u SIN Ho YoUMS pajuawajdwj L0 MH7HVd 8cLLINO 8cLLWO BZLLND Ad day Id Jelly ejejdeseq 93 au payoojq pue jequieuo wonog g 93 u moje pesa o jdwany pajuauwajduwij 00d 34 Olu pessaJd sem Jajem se yoayo qp Buunp paddojs mov L0 MH HVd 8cLLINO 8 L1WS 8 L1WS day Id peuinbai si uoneJp u jeuonippe yi euruuejep IM 98S G 49849 qp uuopeg pajusuajdu L0 MH HVd 82LLINS 8cLLIWNO 8ZI LWO day ld p nb si uoneip u jeuonippe J auwWayep IIIA 98S S YIBYI qp uuoneg pajuawsidwy LLO MH HVd 82LLIND 8cLLWO ZZLLINS dems 23 Jaye Ajjeuiwou sasse boJd UONEJP Y JI Yoayo sesind pejueuuejduuj mojs Gz ses nd pides c uleBe uoneup u jenu woad LLO MH HVd 8cLLINO 8ZLLNS Z2LLW9 dems 93 Jaye Ajeulwou sesseboud uonejp u ji eu p lu uu duuj MOIS SZ sas nd pidei ureBe uoneJp u LLO MH HVd LLLND L1WS LIND J9jEM am 0 wou Buueyns 1nq saye eq Ins uf La ui sjuejd ay L sasind pide 00 urebe g jo UONBJP Y WIOLSd pajuawejduy uoneBiseau enjre DE Jo Weg EP G 10 ajsem eq Pinoys uoneurseg Os spaau ji USYM usa WO Jajem sief 1eyipiunuep ay ji Auen 0 18e jurod 9s 9 0 Wojad sAep G Joj ajsem eq pjnou
80. PEEK plate removed I nnne 12 52 EG Wl EE 13 5 2 1 Before opening bottom Plate uuu oto eder a ote ehe 13 5 2 2 Bottom plate and PEEK plate removed I n n enne 13 5 2 3 uc c 14 6 WATER RESERVOIRS mau EE 19 ST MOOD Am 19 G2 FMOO9 19 7 RBLSS le RUE BEE 22 7 1 Inspection and Functional Test u uuu ttr re tice a rem cu x bar dok a aa edel 22 7 2 Correct RBLSS QDs by swapping Fresh and Waete enne 23 8 INSPECTION OF MULTIGEN EXPERIMENT CONTAINERS nennen 23 9 eeh EE 24 is Dok Nr No EMCS RP 0000 151 DOR Trouble Shooting for EADS Ausgabe lssue 1 Datum Date 18 8 2008 asEer um SPR 283 Seite Page 3 von of 24 1 INTRODUCTION This report describes in detail the trouble shooting proceedings based on the Trouble Shooting Plan RD6 which were undertaken to find the root cause for the failed watering of CWRW ECs 2 REFERENCE DOCUMENTS RD1 EMCS RP 0000 124 DOR issue 3 Fault Tree Analysis RD2 EMCS MA 0000 004 DOR issue 4 Water Reservoir Filling Procedure RD3 EMCS PR 0000 006 DOR issue 2 RBLSS Module Maintenance Procedure RD4 EMCS TP 4000 007 DOR issue 2 EC Functional Performance Test Procedure RD5 N USOC PRO 002 RD6 EMCS PL 0000 010 DOR issue 1 Trouble Shooting and Recovery Plan SPR 0283 RD7 EMCS TP 4000 029 DOR issue 1 CWRW EC Leak Test P
81. PERIMENTS TITLE Procedure for Filtering of Zeolite PREPARED BY Geir Omdal Date 930 002 CHECKED BY Bj rnar Vasenden Date RO Date 03 0 Zee 4 Date 3 25 702 F PRODUCT ASSURANCE Marian N Melle PROJECT MANAGEMENT Gelr Omdal The information contained herein is proprietary to PROTOTECH AS and is to be used by the recipient solely for the purpose of which it was supplied It shall not be disclosed in whole or in part by any other party without the written permission of PROTOTECH AS Doc no EMEX PR MULT 04 PRO prototec h EMCS Experiments Int Doc no PR_30193_PR_05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 ISSUE STATUS Page 3 of 19 ISSUE STATUS The status list shows which chapters are included which changes are introduced valid issue and issued date Ch no Page no 2 Change isue Date Ch 6 App A and Editorial updates based on comments App B from ESA mr ope The information contained herein is proprietary to PROTOTECH AS and is to be used by the recipient solely for the purpose of which it was supplied it shall not be d sclosed in whole or in part by any other party without the written permission of PROTOTECH AS EMCS Dac no EMEX PR MULT 04 PRO protote C h Experimente Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Projet 30193 CONTENTS Page 4 of 19 CONTENTS
82. PROTOTECH AS 2 e Doc no EMEX PR MULT 04 PRO prototech EMCS Experiments Deene pr 30195 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 APPENDIX C Page 19 of 19 APPENDIX C PROCEDURE SIGN OFF SHEET Item Part No Serial No has been filtered in accordance with the foregoing procedure d 23 ENGINEER GRETE RAkvAAG Sign Ge eo Date Ha Pera lp PRODUCT ASSURANCE CARINA HBERG Sign Gonn Ber Date Meg V Zerre l ud V PROJECT MANAGEMENT Sign Date CUSTOMER REPRESENTATIVE Sign Date where applicable The information contained herein is proprietary to PROTOTECH AS and is to be used by the recipient solely for the purpose of which it was supplied It shall not be disclosed in whole or in part by any other party without the written permission of PROTOTECH AS N USOC PRO 005 H USOE AM CWIRW Flight Sample Hardware Packing Transport issue 2 Sh La and Check Out Procedure Date 27 02 2008 Page 37 of 58 Annex 2 Certificate of Arabidopsis Thaliana Seeds Separate certificates for Wild Type GM GUS mutant lefty and mutant hmg N USOC PRO 005 H USOG m CW RW Flight Sample Hardware Packing Transport issue 2 Marvielza Bar aar nd trien tere LES and Check Out Procedure Date 27 02 2008 A a Page 38 of 58 T Tohoku University a D Aobayama January 28 h 2008 3 CH Aoba ku d i Sendai aS JAPAN 980 8578 TOHOKU Phone 81 22 795 67
83. SOC REQ 025 Issue 1 Date 28 11 08 Page 27 of 30 Illumination 1 Illumination during the experiment run is Rotor A Varying light duration and To reduce stress lights off or reduced PI ESA 1 CWRW EIR Exec 15 16h 8h day night cycle dark intensity intensity 2 CWRW EIR Exec 16 2 The light intensity should be 50 w m2 for Rotor B Varying light duration and To increase growth continuous lights the first 20 days and 75w m2 for the intensity remaining days See Appendix 6 for details Gravity level 1 Gravity level rotor A rotating 1xg at Rotor A Varying g ISS Power Issues Payload 1 CWRW EIR Exec 13 2 CW RW EIR Exec 5 1 the surface of PCC lid rotor B static micro g Rotor B Varying g Hydration issues EMCS PAR HW 01 1 led to hydration attempts at different g levels Anomalies and ESA PI 2 The Rotor A will stand still until watering See Appendix 6 for details agreement is complete for ECs with WT and GM wild day 4 Experiment duration The experiment duration is 43 days 10 The experiment lasted for 47 days Inability to hydrate properly Anomaly PAR Payload CWRW EIR Exec 07 days but due to late germination the plants were estimated to 23 days at the time of harvest EMCS HW 001 1 Anomalies and ESA PI agreement Observation target and frequency AP03 1 CWRW EIR Exec 09 2 CWRW EIR Exec 09 1 1 Each EC is observed by still image grabbing every 1 hour 2 Two EC p
84. T Zi a Quality Assurance Date PHOS Ft40qq n PI MOD MOR Maja Rostad _ L Felony 2008 Engi neer Dat M O92 Frenn HOw Font MOQ and FHIOO TD f a ss 04 02 deed _ x N USOC PRO 002 AP04 Ingevation and Crazterity Appendix 4 Issue 1 3 Moulding and Sterilization of Date 23 05 2007 PVA Membrane Page 40 of 56 Appendix 4 Moulding and sterilization of PVA membrane J N USOC PRO 002 AP04 lngoyatioo and Creativity Appendix 4 Issue 1 3 Moulding and Sterilization of Date 23 05 2007 PVA Membrane Page 41 of 56 Appendix 4 Moulding and Sterilization of PVA Membrane Prepared by Reference Issue Date of Issue Status Document type Distribution Suzanne verlie 1 3 23 05 2007 Procedure souBinssy Ayjenty uoneoo ee A0 pauwoped V V SEP 37190 ET 8010 kI wA MANDE ais ss uleqs Jo Buise uwnjo gt Suy 482805 uwnjoj ui aseg 1001 BupinoWw VAd SU JO Sued A pajquiassy NUR ww NIS ZS Led jeajs jo JejeWelg joo Buipjnoyy pezijeioeds asn pouuojuag Yleway oJoyd uogeysuowsg uonduoseg dag 200 Odd D0SN N 88S sjlEjep OJ SPI DDd au u ejou YDES JO apew eq jjeys suelgwew VAd euo 29d Jaqueyj uongAninO 1UBId vebynn Sy ui spees sisdopiqgjy Z qowu ri Jeu eueJquietu vAd jouoo y AUIAA Og eu pinow o mou sequosep einpeooJd Burwoljo eu J eueJquiej VAd jouooaje jAutAAjod jo uone
85. TOTECH AS Doc no EMEX PR MULT 04 PRO prototech 4 EMCS Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 SCOPE Page 5 of 19 1 SCOPE This document describes a procedure to filter the Zeolite according to agreement from EMCS Experiment Batch 1 Muitigen 1 Phase II Flight Safety Review no Zeolite particles below 0 5mm in diameter Taken from MoM from EMCS Experiment Batch 1 Multigen 1 Phase II Flight Safety Review RD1 During the review the question arose if Zeolite would present a potential hazardous to the crew e g the particles causing eye irritation A response was immediately requested from the NASA JSC toxicologist The response received stated that if the PO can assure JSC Toxicology and the PSRP that dusts have been eliminated and essentially all Zeolite particles in the flight samples with larger than 0 5 mm diameter the Toxicity Hazard Level would be reduced to zero non hazard EMCS E Doc no EMEX PR MULT 04 PRO protote h inii Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Projet 30193 REFERENCE DOCUMENTS Page 6 of 19 2 REFERENCE DOCUMENTS The following documents are referenced Rev RD1 Not available MoM from EMCS Experiment A 27 06 2006 Batch 1 Multigen 1 Phase II Flight Safety Review Table 2 1 Reference Documents The information contained herein is propriet
86. Verified during EST models are kept under the correct configuration and the operation should be in accordance with the EC handbook and the EMCS User Manual applicable to this EST 4 All NCRs with negative impact on CW RW should NCR 002 needs a minor update regarding the water repellent be closed properties after complete dry out seen during Science Test This was due to a human error forgot to rewater before day 20 and will not occur on flight Automatic water strategy This is regarded as normal open work 5 All PARs with negative impact on CW RW should PAR HW 006 needs further testing before closure Testing was be closed performed on EMCS FM on the 25 February 2008 and the results will be presented in the NASA PAR System under EMCS PAR HW 006 The EST participants identified PAR HW 006 as an impact on CW RW operations The EMCS generic Payload Anomalies will be handled separately Doc Nr N USOC REQ 025 EMCS CW RW Requirements Integration Issue 1 Report Date 28 11 08 N USOG m3 d Norwegian User Support and Operations Centre LGS Page 14 of 30 Samfunnsforskning AS 3 3 Overall activities and products related to CW RW Pre flight Operations Activities and products related to set up of the EUE and samples are described in section 3 3 1 Activities and products related to transport of hardware from set up location to KSC are described in section 3 3 2 3 3 1 Set Up and EUE Verification The
87. W 0008 EMCS SSL File Transfer and VPU Command Issue Same as EMCS SW 0003 The improvement of the VPU reset command sequence described in EMCS SW 0003 was developed because it was found that the command queue can be blocked under special circumstances when using the original reset sequence Obs target and freq EIR Exec 09 1 Power cycle of EMCS SPLC VPU when VPU was locked in a busy state 2 EMCS schedule to check and repair VPU lockups A workaround to reset the VPU by ground commanding was developed during MULTIGEN 1 and improved during CWRW 1 Resume VPU capability period with loss of environmental control 2 Loosing images during VPU lockup no loss of environmental control Gravity level EIR Exec 13 Illumination EIR Exec 15 Power interruptions EIR Exec 31 Obs target and freq EIR Exec 09 Samfunnsforskning AS N USOC Norwegian User Support and Operations Centre PS EMCS CW RW Requirements Integration Report Doc Nr N USOC REQ 025 Issue 1 Date 28 11 08 Page 24 of 30 PAR Description of PAR and Impact on science and CW RW Requirements ADO1 Affected Requirement Corrective act and Work arounds during CW RW Impact on CW RW after implemented workarounds Additional requirement affected after work around EMCS SW 0009 Automatic subsystems shutdown due to lost communication between the SPLC and the TCS The TCS was powered down by
88. a rapt pr L 8002 20 22 eiea eunpeo20Jg 1nO 29u2 pue zens yodsue Bupjoed ejempsey ajdwes 1464 MM Ba JOSA N S0O0 OUH IOSN N enjeA enjeA sy ewey jenjoy eouesajoj peunuoN uonduosag dajs enpa20Jd UI UODEUEA ei1npaooJg 8 q 1 8 ejqej ur pejueuinoop eq eYs senpeooid ur y enpeoo04g ay o suoneuea pue seBueuo v Ur UONEMEA eunpe2OoJd 8 8510 G abeq 80022042 aed enpa2oJgd JNQ yosyD pue 2 nssi yodsue1 Buryoeg aiempiey ajdwes il MYM i JOSN N 00 OUd OOSN N Ji wm vanges99g pes peste ong ete N USOC PRO 005 mmm mem CW RW Flight Sample Hardware Packing Transport issue 2 em ATTE rm and Check Out Procedure Date 27 02 2008 Page 16 of 58 9 Failures during Process All failures and non conformities during preparation shall be recorded in Table 8 10 Success Criteria The procedure will be deemed successful if performance of all steps Table 7 has been properly executed and no deviations have been observed N USOC PRO 005 mmm WW CW RW Flight Sample Hardware Packing Transport issue 2 Gan and Check Out Procedure Date 27 02 2008 Waewryins Bue Logger end Geeratinns Cealre Page 17 of 58 11 Procedure Sign off All steps and activities described in the Procedure have been successfully performed with test equipment and articles as stated in Table 5 and Table 6 Failures and non conformities raised during performance of the procedure were Procedure sign off Engin
89. ace it in position above the mini lid hole in the Growth Pot Lid Remove the Inner Hole Punch by sliding it upwards while keeping the Guide Sleeve in the accurate position above the mini lid hole Confirm that the punched piece of Borosilicate Fibre remains in the middle of the mini lid hole N USOC PRO 002 AP05 Appendix 5 Issue 1 3 H USBC A oulding of PVA Me Date 23 05 2007 pore eet M mbrane ES HR um Page 55 of 56 Dorssulicate Cuttin The Mini Lid is fixating the Borosilicate Fibre and the PolyVinylAlcohol membrane The seeds are now enclosed and prevented from undesirable movements 8 Use the Guide Sleeve to gently depress the small doughnut shaped piece of borosilicate down in the mini lid hole Use a stereomicroscope to confirm that the piece of borosilicate is evenly clamping the PVA membrane against the PolyPropylene felt and that the seeds are positioned in the middle the borosilicate hole Finally the Mini Lid is mounted with the designated tool 9 Attention Before the procedure can be repeated for the next mini lid hole position the piece of borosilicate that remains fixed in the Inner Hole Punch must be removed using the thin rod of stainless steel to push it out N USOC PRO 002 AP05 Appendix 5 issue 1 3 Moulding of PVA Membrane Dale 23 05 2007 N Page 56 of 56 DBovositiiccoa Cuttin 2 Procedure Sign off All steps and activities described in the Procedure have been
90. ajsem o ysa Wou Jayem duind LLO MH HVd 660LND 660LND d39 Id Qd sas nd pL6 1541 eu vIYIM 186511 jou PIP dp sesind 00 0 unu y Je pue ejnpeuos uonespAy uejses peiueujejdui LLO MH UVd 660LND Jejem UUM SSTEY 01 JIOMES8H OZH Wou seqni i4 o 1dwayy zdwnq 404 uiu duing 104 utu v 10104 p lu uu duu LLO MH UVd 6601WS p lu uu duu UO xog uo Bx ul ajsem o usaJj WO jejem dwnq 98S 09 Jo peajsui 285 GL 0 xoeq sas nd Jajem uojed 84 6 h sesind f LG 151 ou ULIM 186614 jou pip dp Hom pjnoys Ajueppns uoneJp u ji 1694 e asod 14514 eset pue sas nd Jejem JeBJe uy njsseoons jou uoneJp H 00L SZE sas nd GZt 1541 eui UILNIM JBBBLN jou pip dp 860 IN5 Ex G6 brZ ses nd ege 154 eui utu Jeu JOU pip ap LIOISSTLIGNS e uonisod J0 JBUIBLIO aduBYI 10 UOSEZY aduviyo jo UONdLINSa JO EG H 660LND dey ld ad Met LLO MH HVd 8601WS pajuawejduy LLO MH7HVd 860 LIN5 pejueuejduu 860 LIND p lu uu duu LLO MH uVd 860LND payuswejdu 860LW9 pajuawajdu LLO MH A VA 860LND pajuawajduy CL0 MH HVd 46019 p lu uu l duuj sas nd Mojs GZ PPV g SI Si 3 a 5 e e e o e e El El HEI E a 2 21 3 Oo ol ol oi 6 o 001 Gz sasind gzr 15 14 ay uti 1 BBIn JOU PIP ap 988 CL JO peajsul 985 09 0 ses nd Jajem yed S 5 LLO MH HVd 6 ZEZ sas nd GZE 15414 y UM 13551 jou PIP ap 0 0 Jejunoo asind Jejem ese 00L 6 E sesind eer 18414
91. ality assurance aspects i e correct tracing of procedure variations and non conformances Table 2 Personnel Name Affiliation Contact info Responsibilit N USOC 47 735 90181 U Suzanne verlie N USOC 47 735 90181 Transportation H kon Svare N USOC 47 735 90162 engineer pen Arve Grannevik N USOC 47 735 96095 N USOC PRO 005 CW RW Flight Sample Hardware Packing Transport issue 2 and Check Out Procedure Date 27 02 2008 Page 10 of 58 5 2 Location The procedure applies for all the activities that will take place in the laboratory at N USOC Trondheim Norway and at Kennedy Space Center KSC Florida USA 5 3 Equipment needed Table 3 states the equipment needed for the execution and completion of this procedure Table 3 Equipment needed for this procedure Provided b CW RW ECs with PCCs and samples prepared as documented in AD04 Lint free tissue to clean the ECs Isopropanol for cleaning of ECs Temperature logger Transport boxes with foam Disposable gloves 5 4 Environmental Conditions Table 4 Shows the nominal environmental values expected during packing transport and check out Actual values shall be noted down Table 4 Procedure environmental conditions Parameters values Temperature 4 Relative Humidity lt 40 pp CO consentration Pressure 5 5 Duration Packing at N USOC will take 2 hours Duration of transport from N USOC to KSC
92. anskie ofup to 250 000 7 U s 7734 bj ox pazihath bys fie of zotzrom fan 10 000 ex Fem onion a pa than Syoas oz bth 18 U S 4 1001 ko V Page 1 of 2 N USOC PRO 005 N USOC CW RW Flight Sample Hardware Packing Transport issue 2 Rarwestas Buer Ansgar ped Boersons Centre Yi e and Check Out Procedure Date 27 02 2008 Page 49 of 58 APHIS Animal and 3 PlantHealth y Plant Inspection Service Protection amp Quarantine PERMIT CONDITIONS This permit authorizes the importation of the listed articles under the conditions specified below A copy of this pennit including all conditions must accompany all shipments authorized under this permit 1 The seeds shall be selected from appare ntlydisease and pest free sources free of weed seeds including noxious weed seeds sail and other prohibited matter securely packaged to prevent loss in transit and shall be hand canied to the approved pori s by the permittee or his representative The plant materials shall be ceclaredon the travelers Customs declaration form and presented along with a copyof this permit to a representative of Plant Protection and Quarantine PPQ orby a Customs and Border Protection Agriculture Quarantine Inspection CBP AQI at tle time of baggage inspection 2 Upon arrival in the United States the samples will be inspectedby PPQ or CBP AQI If the samples are found apparently free from exotic plant rests diseases soil and other prohi
93. ary to PROTOTECH AS and is to be used by the recipient solely for the purpose of which it was supplied It shall not be disclosed in whole or in part by any other party without the written permission of PROTOTECH AS Doc no EMEX PR MULT 04 PRO prototech LI EMCS Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 PARTICIPANTS REQUIRED Page 7 of 19 3 PARTICIPANTS REQUIRED Personnel required Engineer Responsible for perform filtering according to procedure filling out step by step procedure Quality Assurance Engineer Responsible for e surveillance of the filtering and that filtering is performed according to procedure Responsibilit Company Dep Engineer Prototech Quality Assurance Engineer Prototech rs Marian N Melle Project Manager Prototech r Geir Omdal SIS a Table 3 1 Participants Doc no EMEX PR MULT 04 PRO EMCS Experiments prototech Int Doc no PR 30193 PR 05 03 05 2007 Issue B Date Procedure for Filtering of Zeolite EQUIPMENT LIST Project 30193 Page 8 of 19 4 EQUIPMENT LIST Prototech ami i Filter jig with mesh opening of 0 5mmx0 5mm Table 4 1 Equipment List The information contained herein is proprietary to PROTOTECH AS and is to be used by the recipient solely for the purpose of which it was supplied It shall
94. ate 16 01 2008 Page 6 of 13 era E aeg Doeratians am NES 3 Scope of Document This document and the Multigen 1 PCC Assembly and Seed Set up Procedure AD09 cover the CW RW PCC set up of seeds in the CW RW FMs This document is based on input from PI rep and JAXA CW RW Team Coordinator The set up will be performed according to the Multigen 1 set up procedure except for the strains of Aribidopsis Thaliana the positioning of seeds number of holes in PCC Growth Pot lid FM numbers and the implementation of the small o ring which all differ from the Multigen 1 experiment These differences are covered in chapter 4 4 Differences from Multigen 1 Set Up Procedure 4 1 Seeds This point replaces step 25 in the Multigen 1 PCC Assembly and Seed Set up Procedure ADO9 in the Cell Wall Resist Wall Set Up 4 1 2 Seed description For Cell Wall Resist Wall 4 different seed strains are used Arabidopsis thaliana wild type WT lefty hmg GUS GMO 4 1 3 Sterilisation of the Seeds Arabidopsis seeds in eppendorf tube e Sterilized in 500ul of 70 ethanol for 5 min e Washed with 500ul of sterilized water 1 time e Soaked in 500ul of 2 5 sodium hypochlorite and 0 5 Tween 20 for 5 min e Washed with 500ul of sterilized water 4 times e Dried on sterilized filter paper N USOC PRO 004 CW RW Seed Set Up Issue Issue 1 Procedure Date 16 01 2008 HL sov WA kanmigize Use Sapper 988 Dperalisns Cesire n Re NC Page 7 of 13 4 1 4 Se
95. au UlYJM 16651 jou PIP dp ses nd mojs 94 PPY LLO MH UVd 00L 6 E sesind eer 151 eu uym 16651 jou pip qp 98S GL JO peajsu 2es 09 0 sas nd Jajem yore co co o o 3 3 3 3 8 E E E E E oj oj oj oj o 0 01 Jejunoo esnd Jejem yesoy Uyd PNPA RIES aep ang bi JO Z bed Jsr wey uonov Bunsayy ssauipeay youne snquunjoo LLO MH UVd LLO MH UVd LLO MH UVd e o LLO MH UVd LLO MH UVd LLO MH lVd e o LLO MH73HVd LLO MH7HVd CN e e e ape ap v o wo a o a pa n O D a ui a LLO MH UVd LLO MH UVd LLO MH YVd s s ai ai s eg 2 2 E El El El El E E E E E 5 5 E Oo Of O oj oj oj Of o LLO MH UVd LLO MH UVd LLO MH7HVd LLO MH UVd LLO MH UVd LLO MH UVd LLO MH UVd avd Palepl vi JO abed vYOLLINS p lu uu duuj POLLWS p lu uu duu OLLWO pajuawajdwij OLLWO pajuawsjdwi OLLWO p lu uu duu OLLWO pajuawajdwi ZOLLIND pejueuuejduuj OLLWO pejueuiejduuj OLLWO pajuswajdwij OLLWO pajuawajdwij OLLWO p lu uu duu COLLINS p lu uu duu ZOLLND pajuawsidwiy ZOLLND pajuawajduy COLLND p lu uu lduu cOLLINO pajuawsjduij a x amp a a a 2 x a a a e e e Q o x a e o l 9 D a G a a c a a a day EOLLND uorssmuqns JO 1E e o e a o c a a a day l
96. ay Id OP LLIND RE SELLND ZELLWO SELLND LIOISSILUGNS joaleq CH LIND pajuewajdw Hr LLIND pejueuiejduu re N 3 J oO e ObLLWO pajuawajdw o o OP LIND pajuswajdwi o 6ELLND p lu uu duu 6E LLNS pajuewajdwj 6ELLWO pajuawajdui ZELLND pejueuiejduu 9ELLIND pajuawajdwi EbLLWO pajuawajdwij OP LIND pejueuiejduu ZELLN 6 LLWO 6 LLND GE LLIND ZELLIN S 3 Sl E EI EI E ol oi o SIJEJS aep anq TIOJEUISLIO Ajajajdwoo yno Aup Aey 810J8q pasanJey aq pinoys pue Jejem Aue Bulava jou ae sjuejq Jejem Aue 186 jou saop Apuesedde yoiym sjuejd ay Jo jno up prone 0 Japso ul paonpa aq pinoys uonejnueA pue seJneladwa Ajisuejur uonguluunjj epJejseK Buiduund asjyeneu Jaye pejeJp u Bureq Jaye esal jou pip pue pajeuBeis aq o wees uonisinbe aew uanbay ei uses aq ueo se SJUSLUSAOUW jue d epJejseK sanul exe pue ses nd ce ueAI6 Bulag Jaye pesa jou PIP pue pejeuBejs eq o waas uonisinbe abew jusnbay el u s aq ueo se SJUSWSAOW jueld Buluuouu sun ses nd ge uan b Bulag Jaye Ye se jou PIP pue peyeuBejs aq 0 wees uonisinbe eBew juanbey ei uses aq ULI se sjuewanow juejd payoyms eqjou ueo z eui Bip se pasn eq jou ued xoeuo qp keplajsak sas nd Jejem S UsAIB Bulag Jaye ejsau jou pip pue payeuBbe s eq o waas uonisinbe eBew juanbe y ei uees eq ue se sjuewanou que d sas nd Jayem 01 ueAIB Bulag
97. bited matter they will be returned for delivery to Kemedy Space Center Florida 3 The seedlings will be grown onan zeolite substzate in orbit on the ISS for 14 days The imported plant materials may not be distributed to any person without permission from this office 4 At the conclusion of the tests all imported plant materials are to be returred to Japan in un opened experiment containers END OF PERMIT CONDITIONS Pe m ItMember P DEP DE DDUS THIS PERMIT HAS BEEN APPROVED ELECTRONICALLY BY THE FOLLOWING PPQ DATE HEADQUARTER CFFICIAL VIA EPERMITS Karen Brady 02 20 2008 WARMING Any alontbn forgaror unvuftoriud wa of thi Fodor Form ubctto chal iy ofep to 230 000 7 U S s TBH DY ox ynichaltb ya fize efzotzom fun 10 000 or imple amar oder inim an Jes E medici li bler phat by Page 2 of 2 N USOC PRO 005 N USDC mw CW RW Flight Sample Hardware Packing Transport issue 2 onn Y and Check Out Procedure Date 27 02 2008 Page 50 of 58 Annex 6 NASA Cover Letter pro forma invoice packing and shipping list and IOT insurance coverage N USOC PRO 005 N USOE TES CW RW Flight Sample Hardware Packing Transport issue 2 mimi LA and Check Out Procedure Date 27 02 2008 Page 51 of 58 National Aeronautics and Space Administration John F Kennedy Space Center Kennedy Space Center Florida 32899 REFERENCE Harmonized Tariff Schedule of the United States Heading 9808 00 8000 Goods certified by it to the
98. cedure Sign off All steps and activities described in the Procedure have been successfully performed Failures and non conformities raised during procedure execution were listed in table 2 Procedure sign off Engineer Date Chai Boa og Feb of Motoshi Kamada JAXA Quality Assurance Date Mae esae OG February At Maja Rostad N USOC N USOC TR 027 N USOC MS CW RW Issue 1 Norwegian User Sagpor and perapoas Ceatre 3 PCC Assembly and Seed Set Up Report Date 13 02 2008 Page 103 of 105 Appendix 3 Pictures from CW RW PCC Assembly and Seed Set Up Fixation of sponge Growth pot sterilization Drying of the sponge YTRET EKLE RRR KSSE KR MA MEKKA KK KAARE Add LA hl d at 1 fi hl uk Wd Implementing top PP felt N USOC TR 027 N USOC A W CW RW Issue 1 Norwegian User Saggori and perapoas Ceatre r Y PCC Assembly and Seed Set Up Report Date 13 02 2008 Page 103 of 105 Implementing PVA membrane on seeds Making doughnuts Ap lying silicone to mini lids N USOC TR 027 N US0C A CW RW Issue 1 Bir Gar Bu C m PCC Assembly and Seed Set Up Report Date 13 02 2008 Page 103 of 105 Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 Integration Report Date 28 11 N USOC 3 g p ate 28 11 08 Norwegian User Support and Operations
99. cember December A G Hydration to watering Mode once a day for 20 seconds schedule to be started 3 21 G A test manually Based on the Delta Pressure and Flow one has to decide if Test C B watering is necessary Plan is needed for how to interpret different variable Procedure W S of pressure and flow Who will decide if watering is necessary Venting of 2 C FMs G R roots Verify Schedules 4 Germination ERM Verify the ability of FMs to support germination Schedules December December S E O Test Test 10 17 A G Procedure K M 6 C FMs W S 1 COM C B G R 5 Run 2 EM Biological Repetition Optimization of Germination Hydration Recycling Test Schedules January January G A Test 9 20 W S Technical Test PAR HW 0006 Procedure PAR SW 0001 SW 0003 4 M ECs 6 EST Table Verify functionality of all procedures for set up and transport all EMCS Schedules January February S E O Top schedules all crew procedures etc Test February M R Procedure 7 Technical ERM PAR HW 0006 TBD November November Testing 5 14 January February 10 p 8 Technical FM PAR HW 0006 SW 0001 TBD December Testing Norwegian University of N USOC NOT SEO 001 Science and Technology Issue 1 CW RW Test Plan Date 09 01 2008 Page 2 of 2 CW RW Test Plan Timeline Table 2 The table illustrates the CW RW test plan timeline including dates reserved for RT Set Up and Experiment execution on orbit Green blocks represent planned t
100. ctivities related to CW RW Post flight Operations 21 4 Payload Anomalies and incidents which led to altered Experiment Requirements 22 4 1 PAR Summary 2 as bid aa kalit 22 4 2 Altered Requirements qm 26 4 3 Main Reason for OE REE 28 eene EE EE 29 APPOMANC S EE 30 Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 Integration Report Date 28 11 08 Page 6 of 30 Samfunnsforskning AS Norwegian User Support and Operations Centre Acronyms 1xg The level of gravity found on earth AD Applicable Document CM Configuration Management CW RW Cell Wall Resist Wall EC Experiment Container EM Engineering Model EMCS European Modular Cultivation System ERM Experiment Reference Model ESA European Space Agency EUE Experiment Unique Equipment FM Flight Model GM Ground Model H W Hardware ISS International Space Station N USOC Norwegian User Support and Operations Centre PCC Plant Cultivation Chamber PA Product Assurance PI Principal Investigator PIM Payload Integration Manager PM Project Manager QA Quality Assurance QD Quick Disconnect RBLSS Rotor Based Life Support System RH Relative Humidity RD Reference Document URC User Requirements Collection Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 Integration Report Date 28 11 08 N USOC Wem Page 7 of 30 Samfunnsforskning AS
101. d ad day Id Ad dey Id Ad 9 8 9 8 8 amp SE aj a amp aj a aj a al aj a Qj a a a a aj a of ap a snjujs aep 20 STOT Dom ui UM015 aq pjnoo YDIYM g 40104 uo sjuejd OU aye 8484 USYM 10 01 SI uo 6x uni o esues yew jou seop 1 pue Juswow SU JE 40 0J V au uo ele sBui pees pejeuruue6 Ajuo au Bo ut UM015 aq p no2 uoIuM g Jojo uo sjuejd OU aje alay USYM JO OJ SI uo BX uni o esues yew jou seop 1I pue juewou eu je 10 01 V y uo eue sBui pees pejeuiuue6 JUO eu L sas nd Q04 154 SY uli Je561 jou PIP dp Sie JO seqn Bui amp Bojoun Burdjeu si AjAeJ6 ji sayaq sseJ6o1d jy6tw uonejpAH ses nd 005 S114 Su uli 8651 Jou PIP dp Sie JO seqni Bui amp Bojoun Buidjay si Ay neu ji sayeq ssesBoud jy61w uongjpAH ses nd 00 1541 ou utu Je551 JOU pip dp Sie JO seqni Bui amp bojoun Burdjay si Ay neu ji Jeneq ssesBoud 1uBitu uonejp H 001 922 sesind 9Z 1811 au utu Jeun jou pip dp Sie JO seqn Bu ojoun Buidjay si Aj eJ6 ji senaq ssa5o1d yyBiw uonejpAH 001 v19 sesind p12 1514 a4 ulm 12651 JOU pip dp ses nd y 9 1511 y uiu 186611 JOU pip dp V ui paddems aq IM v 93 eseo ui p p u al asegejep M N suonisod 23 Jeyo au vi Ajeunyou sseJ6o4d jou pip uonejp H suonisod 93 Jeujo y ur Ajjeuiwou ssaJ6o4d jou pip uoneJp H suonisod 23 Jeyjo y u Ajjeuwou sseJboud jou pip uoneJpAH suonisod 93 1340 au ui EUILUOU sseJboJd jou pip UONBJP H suonisod 93 Jeujo au ur Aj euruou sseJ6oJd jou
102. de the seeds in 20 06 07 1 Step 25 place on the centre of the PP felt instead of Glass funnel Add additional wiping of PCC Growth Pot lid to 1 20 06 07 Step 30 remove excess grease Add step to bring EC into the flow bench and surface 1 3 20 06 07 21 Step 33 sterilize them both inside and outside with ethanol Add implementation of circular autoclaved PP felt in 1 3 20 06 07 21 Step 34 the bottom of the PCC Implement check of correct alignment of PP felt on 1 3 20 06 07 21 Step 35 outside recession of growth pot E 3 23 Only three of four screws will be measured for 20 06 07 tarque since the tool was to big for the fourth position EC Sealing frame green will be inspected for dust particles and coated with a thin layer of Wacker Silicone High Vacuum Grease 20 06 07 Step 38 39 N USOC PRO 002 Multigen 1 Issue 1 3 PCC Assembly and Seed Set Date 20 08 2007 Page 4 of 56 Up Procedure Table of Contents En EE 5 TESS COP f pakaq S La u 2 Su Q nta q aa u qaa p guess 6 2 Pip PD 6 2 1 Applicable Documents ANEREN 6 2 2 Reference Documents u L Lu nG ak QD etta a hatt baeo Su aS nass ens 6 3 Multigen 1 Experiment Design ssssseeeeeeneeee nennen enema 7 4 Ground Support Requirements nn sssssrsssssssssses 8 S SGCUD ATtCIOS e ns o HER TO DE ia i de eeu duubduausdexocansetedbuessbaves 9 6 Environmental
103. dule with counter set to 24 A total of 125 pulses given Manual pumping 10 times 1 minutes valves switched between each pulse Started hydration schedule with counter set to 24 Resat counter to 25 when it reached 96 A total of 121 pulses added Resat counter to 69 when it was at 98 Let run to 100 A total of 225 pulses given Pumping for 5 minutes without switching the valves between the 5 1 minute pulses tarted hydration schedule Resat counter to 47 after 3 pulses A total of 82 pulses were given Performed one 5 minute pulse for preparation of Added 5 water pulses Added 5 water pulses manually manuall pulse A total of 61 pulses given Added 5 water pulses Added 5 water pulses manuall manuall schedule with counter set to 49 Resat counter to 83 when it reached 28 A total of 94 pulses were given EC relocated to position B2 Started hydration schedule with counter set to 24 Resat counter to 18 when it reached 7 Resat counter to 78 when it was on 28 Schedule continued to 100 pulses A total of 99 pulses were given Started hydration schedule with counter set to 49 Resat counter to 78 when it reached 28 A total of 99 pulses were Initial hydration started 100 rapid pulses given Started hydration schedule with counter set to 49 Resat counter to 83 when it reached 28 A total of 94 pulses were given dP check performed PCC full of water Bottom chamber flooded dP ch
104. e 6 1 The fresh water bag was kept in the original flight state for later use in the end to end test see chapter 4 2 4 3 Calculated fresh water content from overall weight is 201 g a proper value in comparison to the expected value of between 184 and 254 g acc Table 6 1 No blockage or any other failure was found The Water Reservoir was closed again using the original parts and it is regarded ready for filling before launch dis Dok Nr No EMCS RP 0000 151 DOR Trouble Shooting for EADS SPR 283 Ausgabe Issue 1 Datum Date 18 8 2008 StErium Seite Page 21 von of 24 Table 6 1 Analysis of CWRW water budget in gram calculated from the number of commanded pulses only blue red values are relevant positive amount put into reservoir negative taken from reservoir seconds pulses before WR exchange afer WR exchange FMOOT fresh resh to HU 59 So 2267 3049333333 3702 49 36 esh to EC 26603 17735 19641 261 88 6962 92 82666667 resh to Waste ao 18 ea 24 9 49 DH to HU ft 24 sy DH to EC a 2654 era ro 2944 oo vm ms m 8 O o n po T I Do 0 0 0 Op 587 3 6743 89 90666667 2067 2756 CO i HI oO aj gt l ola o p HU to DH esh to HU resh to EC resh to Waste DH to HU DH to EC m el I gt o ER 7 a m m Dj wj ofw an Fe
105. e DE mur um T m Bee K Eur fl RETI k n s Tm b i Kg KA Bn LEM LET ea ke BA Le MESE s ar EE L p uens 7 mE A 7 uongmsguoo dnjes pes MY MO T AQL LI i e Em o 9 EEEEE a D o a 2 o e 2 Wu S c P Oo S n EEE e c5 o EEE E s a S sn n sn 9 uens uens 2 2 o o O o L ET ke CP EL JO 6 beg 8002 10 91 0 enpeoolg deit 205N N enss enss dn jes pees MM MO PIT YOSIY 21009 00 O4d 90SN N ONLLN CW RW Seed Set Up Procedure Back N F Figure 1 Number of holes in PCC lid Number of seeds WT 7x2 4x4 30 GUS 7x2 14 lefty 15x2 30 hmg 15x2 30 Figure 2 Number of different types of seeds N USOC PRO 004 Issue Issue 1 Date 16 01 2008 Page 10 of 13 N USOC PRO 004 CW RW Seed Set Up Issue Issue 1 Procedure Date 16 01 2008 U Les ms te Foren wea toerien ege O Page 11 of 13 5 Test Procedure Variation Fill in All changes and variations to the Procedure shall be documented in Table 2 NTNU N USOC PRO 004 Social Research Ltd CW RW Seed Set Up Issue Issue 1 N USOG W Nerwegiza iret Sopgert nad Ureralioas testre MEAN Procedure Date 16 01 2008 lim Page 12 of 13 Nominal Tolerance Actual Value Value Table 2 Cont Test step Description N USOC PRO 004 Issue Issue 1 Date 16 01 2008 Page 13 of 13 CW RW Seed Set Up Procedure 6 Pro
106. e Integrated Operations Team Astrium herewith authorizes the N USOC to initiate and perform the transport of the CW RW EUE packed in 3 boxes at a total recurrency value of US 821872 from the N USOC in Trondheim to NASA Kennedy Space Center Florida The transport is fully insured by the EADS N V Insurance Policy for the ESA Manned Space Programme against all risks of physical loss of or damage to property during transportation and storage in transit NASA KSC CAPPS Transportation Management Export Control needs to be notified in due time prior to arrival 3 4 days and provided with the Proforma Invoice Flight name of the traveller Date of Birth country issue date expiration date and Passport in order for them to prepare a Verification of Duty free and Entry free Entitlement for NASA Shipments which will then be forwarded electronically back to the traveller to enable Customs processing at the traveller s US gateway airport where the traveller processes through US Customs To avoid paying US duties and taxes on the hand carried item s up to 25 of the value of the item s depending on how the Customs officer decides to classify the item s the traveller will be responsible to apply for the support request for handcarried articles to NASA in the time frame indicated above Note Handcarrying of Hardware in aircraft passenger compartments should be avoided to the maximum extent possible due to recently increased security meas
107. e Plant Cultivation Chambers PCCs Inside FMO98 there are a total of 7 Arabidopsis thaliana GM GUS seeds placed in 7 holes in the lids of the Plant Cultivation Chambers PCCs Transport box 3 Inside FM099 there are a total of 4 Arabidopsis thaliana Wild Type seeds and 15 Arabidopsis thaliana mutant lefty seeds placed in 7 holes in the lids of the Plant Cultivation Chambers PCCs Inside FMO100 there are a total of 4 Arabidopsis thaliana Wild Type seeds and 15 Arabidopsis a mutant hmg seeds placed in 7 holes in the lids of the Plant Cultivation Chambers PCCs The ECs are closed and can not be opened The seeds can not bee seen as they are implemented inside the PCCs Each of the ECs wil be kept inside a transparent Ziplock bag which allows visual inspection Location Tel 47 73 59 01 81 Adress NO 7491 Trondheim Dragvoll Alle 36 Fax 47 73 5901 77 Org No NO 374 767 580 N USOC PRO 005 N USDE Tw CW RW Flight Sample Hardware Packing Transport issue 2 Rarwtelas Bear Les and ByersDens tests W and Check Out Procedure Date 27 02 2008 Page 55 of 58 NTNU ae Bana rpikn User Sapyori ent Operations Cestre Social Research Ltd ress Location Tel 47 73 59 01 B1 NO 7491 Trondheim Dragvoll Alle 36 Fax 47 73 5901 77 Org No NO 974 767 860 N USOC PRO 005 PETS m CW RW Flight Sample Hardware Packing Transport issue 2 Barwtalan Bier Lunner and Sueratieas Castro S and Check Out Procedure Date 27 02 200
108. e rationale for this request was that After the Water Reservoir Replace EC B1 was hydrated and indicated a full growth pot but the seeds in this EC had not germinated A probable cause was that the seeds had been added small amounts of water prior to the reservoir replace but not sufficient to germinate and after this they had deteriorated This indicated that the position B1 could support hydration and it was proposed to exchange EC A1 with the EC B1 to rescue the small seedlings present in EC A1 Crew was also asked to exchange EC A2 with EC B2 to support troubleshooting analysis of the hydration issue The new positions of the CW RW ECs after the EMCS CW RW EC Exchange on May 5 can be seen in table 3 8 The green filling indicates positions were there were seedlings An updated experiment database was also created to support the new EC configuration Table 3 8 CW RW EC and seed assignment list after CW RW EC Exchange EC Location Flight EC A1 Flight EC A2 Flight EC A3 Flight ECA4 BEEN FightecCe2 EE EC FM 97 99 94 96 92 95 98 Seed Type WT lefty WT hmg WT GUS WTilefty WT hmg WT GUS Hydration of the small seedlings present in position B1 was tried again but unfortunately without any indication of successful water supply Figure 3 4 shows the seedlings in B1 two days after the last watering attempt In Appendix 5 a summary of hydration for all ECs can be found M ch 082
109. e recipient solely for the purpose of which it ied It shall not be disclosed in whole or in part by any other party without the written permission of PROTOTECH AS Doc no EMEX PR MULT 04 PRO prototec h za EMCS Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 Description Page 11 of 19 3 Start filtering the Zeolite by moving the FJ from side to side by using your hands See picture below Continue this to you observe that no more Zeolite is falling through the mesh in the FJ The QA engineer shall also observe and agree when the filtering is sufficient Move FJ from side to side Filtered Zeolite above 0 5mm diamter
110. e test to the end to end test with EC FM099 9 ACRONYMS EC EUE GSE HX PCC QD RBLSS RH Experiment Container Experiment Unique Equipment experiment related hardware inside EC Ground Support Equipment Heat exchanger Plant Culture Chamber Quick disconnect Rotor based life support system relative humidity
111. easseassanssseosaooossossanossonssoosonooonnnn 5 l Dok Nr No EMCS RP 0000 149 DOR Failure Analysis n EADS mor d Report SPR 283 Ausgabe lssue 1 Datum Date 1 8 2008 SSEri urm Seite Page 3 von of 5 1 INTRODUCTION This report summarizes the result of the activities based on the Trouble Shooting Plan RD6 to find the root cause for the failed watering of CWRW ECs After inspection and intensive testing of all returned parts inspection of four returned MULTIGEN ECs analysis of CWRW water commanding sequence the root cause could be clearly identified The attached as run procedure describes all activities in detail The main results are summarized below 2 REFERENCE DOCUMENTS RD1 EMCS RP 0000 124 DOR issue 3 Fault Tree Analysis RD2 EMCS MA 0000 004 DOR issue 4 Water Reservoir Filling Procedure RD3 EMCS PR 0000 006 DOR issue 2 RBLSS Module Maintenance Procedure RD4 EMCS TP 4000 007 DOR issue 2 EC Functional Performance Test Procedure RD5 N USOC PRO 002 RD6 EMCS PL 0000 010 DOR issue 1 Trouble Shooting and Recovery Plan SPR 0283 RD7 EMCS TP 4000 029 DOR issue 1 CWRW EC Leak Test Procedure RD8 EMCS PR 0000 010 DOR issue 1 RBLSS QD Exchange Procedure RD9 EMCS TP 0000 135 DOR issue 1 RBLSS System Proof Test Procedure 3 SUMMARY OF RESULTS The returned parts ITEM CWRW history initially on Pos A1 germination but no successful watering finally on Pos B1 FM092 EMCS EC 401 000 watering re
112. eck performed PCC full of water Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 Integration Report Date 28 11 N USOC B a g p ate 28 11 08 Norwegian User Support and Operations Centre Page 1 of 1 Samfunnsforskning AS Appendix 6 CWRW Implemented changes LLO MH UV d 960LIND 960LWD p lu uu duu yo amod swajsAsqns SON 01 peal pinoo U3IUA syeed Josues ZO Plone 0 ZN9 PP 0 l q sIAPE SI ji J UA A E Je si jene Ajayes ZO 960 LIND uo N 1senbay w o o LLO MH UVd 960 LIND pajuawajdui LLO MH HVd 960LWD p lu uu duuj LLO MH UVd 960LWD pajuawsjduj LLO MH UVd 960LIND pajuawsjdwj LLO MH7HVd L60LND pejueue duu LLO MH lVd L60 LIWO p lu uu duu LLO MH HVd V60 LWO pajuawsjdwj LLO MH UVd L60LND pajuawsjdwij LLO MH7HVd L60LND pejuawajdu LLO MH HVd L60LND OLND pejueuiejduu LLO MH UVd L60LND p lu uu duuj LLO MH UVd L60LND pajuawsjdwj LLO MH AVd L60LND pajuswajduy LLO MH HVd L60LND pajuawajduy LLO MH UVd L60LND pejueuiejduuj LLO MH YVd L60LND pajuawajdwij LLO MH AVA L60 LIND pejueuie duu LLO MH UVd L60LND p lu uu duu LLO MH7HVd L60LND pajuawejdu 0L0 MH YVd 060 LIND pajuewajdw 0G b6L sesind pyz 1514 ayi utum 16661 JOU PIP dp ege MOU JeJEM SONS AWAD MAID Jo Sanbay 9 OO Le day Gr 481 sesind zez 18414 eu utu 66601 jou pip ap Spe MO JejeM SONS AWARD Me49 10 jsanboy C60LND C60
113. ed launch date of March 11 2008 from Kennedy Space Center Florida Suzanne Overlie Passport 26011775 is intending to be traveling on the flights listed below 03 03 08 KLM KL1172 from Trondheim Airport Waernes Norway TRD to Amsterdam AMS MartinAir MP 635 from Amsterdam AMS to Orlando Florida MCO The N USOC NOT SEO 003 experiment hardware is valued at USS821 872 00 will be either hand carried checked as baggage or checked as cargo by Suzanne Overlie A DO NOT OPEN label is affixed to the containers Opening the containers will render the experiment useless The N USOC NOT SEO 003 experiment hardware is authorized for duty free entitlement under HTSUS Number 9808 00 8000 and entry free entitlement under U S Note 1 replicated in Reference box above Additionally a Permit to Import Plant or Plant Products for Experimental Purposes number PDEP 08 00045 issued on 02 20 2008 by United States Department of Agriculture is attached if you have any questions concerning this matter please feel free to contact me at the number below or Rose Ogden at 321 867 1963 or on her personal cell at 321 720 Manabu Ishii at 321 867 1763 Sjficerely Melly Caste Lestey Caro Transportation Officer 321 867 2975 N USOC PRO 005 mmm m CW RW Flight Sample Hardware Packing Transport issue 2 Wiederen and Check Out Procedure Date 27 02 2008 Page 52 of 58 J H USOG We NG NTNU Winergas User Support acd Gze
114. ed set up in the CW RW PCC Flowerpot Table 1 shows the set up of the CW RW EC Flight Models EC FM and PCC FM with the correct seed strains The EC FM and PCC FM are given in row 1 and row 2 of Table 1 Figure 1 and 2 show minilid locations number in the CW RW PCC flower pot where the different seed types are located The minilid location number from 1 to7 is found in column 2 in Table 1 In column 3 the strain names can be found and the number of seeds in the minilid is given Num N USOC PRO 004 Issue Issue 1 Date 16 01 2008 Page 8 of 13 CW RW Seed Set Up Wa Procedure Berstegiss User 5730271 ana Destalisas Ceatie un 4 2 O ring This point replaces step 32 in the Multigen 1 PCC Assembly and Seed Set up Procedure ADOS in the Cell Wall Resist Wall Set Up 4 2 1 Implementation of small o ring Implement the sterilized small o ring on the Growth Pot Lid instead of on the water nipple ADO7 6 3 1 step 1 and 2 This is to prevent the o ring from being damaged 9993 utum wor 4272 n mau 4m L mau Am py mau Lp mau LM Ke 9 4 819 1m 1212 4 AM mou 192 1 1210 imp Im mau PH sopog wo Te SAK Z Pag smoH bo 7 pwe EET uejd 0 Buipyo22e dn jeg Ap ARR L ud E LL BENE oL A acle EEE es FOREX ee 84 4 9002 Gr 1W 8002 Fs 81 1 8002 cuu arcu zziL 8ODZ EINE UNSER RE RK L z unu L A MERE Ed men pay D IM I L esch IM L
115. eer Date Hakan Svan 6 3 2008 Engineer Date anme UA 6 3 2008 Engineer Date N USOC PRO 005 N USOE mem CW RW Flight Sample Hardware Packing Transport issue 2 IT e and Check Out Procedure Date 27 02 2008 Page 18 of 58 Annex 1 Filtration of Zeolite as performed prior to Multigen 1 experiment flight set up June July 2007 EMCS EXPERIMENTS Procedure for Filtering of Zeolite N u o m K O z2 eg v a 8 a n LI o m sr H 2 n a d LI Doc no EMEX PR MULT 04 PRO prototech IP EMCS Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 Page 2 PROJECT EMCS EXPERIMENTS TITLE Procedure for Filtering of Zeolite PREPARED BY Geir Omdal Date 0380 007 CHECKED BY Bj rnar Vasenden Date O3 0520T ps PRODUCT ASSURANCE Marian N Melle Date 03 09 Zec 4 PROJECT MANAGEMENT Geir Omdal Sign Date BIT The information contained herein is proprietary to PROTOTECH AS and Is to be used by the reciplent solely for the purpose of which it was supplied It shall not be disclosed in whole or in part by any other party without the written permission of PROTOTECH AS Doc no EMEX PR MULT 04 PRO prototech P EMCS Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 ISSUE STATUS Page 3 of 19
116. egration Issue 1 Report Date 28 11 08 N USOG m3 a e Norwegian User Support and Operations Centre s Page 21 of 30 Samfunnsforskning AS 3 5 Overall activities related to CW RW Post flight Operations The 3 KFTs were returned on shuttle 1J on June 14 in double cold bags and recovered by PI representatives present at SLS Some hardware was also returned to EADS Astrium for troubleshooting the watering issues seen during the CW RW experiment run Including EC FM 099 Water Reservoir FMOO1 and RBLSS module FM008 which were all removed from the EMCS Water Reservoir FM009 which had been used at the start of CW RW and exchanged by crew was also removed from stowage and returned on 1J One additional EC FM092 was returned for scientific reasons and on June 16 PI rep performed harvesting from FM092 The KFTs and harvested material were shipped to Japan in cold boxes Samfunnsforskning Norwegian User Support and Operations Centre EMCS CW RW Requirements Integration Report Doc Nr N USOC REQ 025 Issue 1 Date 28 11 08 Page 22 of 30 4 Payload Anomalies and incidents which led to altered Experiment Requirements 4 1 PAR Summary There were a number of unplanned abruptions to the EMCS environmental control during the on board Cell Wall Resist Wall Experiment run table 4 1 The major impact on the science was caused by repeated problems with hydration of the ECs EMCS HW 0011 the airflow problem in EC B3
117. en filtered in accordance with the approved procedure to be signed and dated by Engineer Quality Assurance Representative and Customer Representative where applicable in Appendix C 2 Doc no EMEX PR MULT 04 PRO protote C h um Expertments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 TABLE LIST Page 16 of 19 11 TABLE LIST Table 2 1 Reference Documents ERE e ale jadin Pre EU 6 Table 3 1 Participants PT D EH 7 Table 4 1 Equipment List e reel ies S Ee de en AEN gen dee ec Sv HO31O0 109d JO u jssiuad WaNLIM IUJ INOUNM Aued mung Aue Ag Hed ul Jo ajoym ui pasopsip aq jou EYS 11 paliddns sem 3 youm yo asodind atu Joj Ajajos JuaidipeJ au Aq pasn eg 03 si pue SY HJHLOLOUd 03 Asejaudoud si US pauyeyuon voneuwojui SYL 2 SCH soud yw uondinsog des l33HS NOILVIAdG JANGIAIOUd V XTQNdddV er Jo LI aBed V XIaN3ddV E6TOE fold ZOOZ SQ O ee g enss 83110982 jo Buniaylg 403 e1npe2o4d SO Hd EGTOE Yd ou 20q UI s1ueuiLiedx3 SOWA Oud PO LINW Yd X3W3 ou 2og Doc no EMEX PR MULT 04 PRO prototech Va EMCS Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 APPENDIX B Page 18 of 19 APPENDIX B NONCONFORMANCE LIST NCR DESCRIPTION The information contained herein is proprietary to PROTOTECH AS and i
118. er LO wean o Domm A UN Hm NONCONFORMANCES senennennnuvnnnnunsnnnnnennnnunesunnennnnennnnnannennrnnnennennnennensrnnener LO PROCEDURE SIGN OFF SHEET aevnesnnnnuunnnnnvensnovunnvanvenvnunsnnvsnvevnavnsuvnsuveununver 15 l o 11 TABLE LLIST i16 APPENDIX A PROCEDURE DEVIATION SHEET svansvavuvsnnnnnrnnnnvunnnnnenunnnnnsnnnnnener 17 APPENDIX B NONCONFORMANCE LIST unsesnavsunovnonnnsnvennsnvennennonannnevnnnneneunnnn LO APPENDIX C PROCEDURE SIGN OFF SHEET ursansannenononannnsansnsnnnnuennnnnnenennennenn LO The information contained herein s proprietary to PROTOTECH AS and Is to be used by the recipient solely for the purpose of which it was supplied It shall not be disclosed in whole or in part by any other party without the written permisslon of PROTOTECH AS Doc no EMEX PR MULT 04 PRO prototech 4 EMCS Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Projet 30193 SCOPE Page 5 of 19 1 SCOPE This document describes a procedure to filter the Zeolite according to agreement from EMCS Experiment Batch 1 Multigen 1 Phase II Flight Safety Review no Zeolite particles below 0 5mm in diameter Taken from MoM from EMCS Experiment Batch 1 Multigen 1 Phase II Flight Safety Review RD1 During the review the question arose if Zeolite would present a potential hazardous to the crew e g the particles causin
119. er Serial No Total readings since new Sample Rate Logged readings Issue Time Date Owner Manifest User Manifest 1 User Manifest 2 Signature 1 Print Name Date 14 0 C 1 mar 08 00 00 4700000005068D41 21532 10 mins 4096 29 feb 2008 9 56 49 AM Not Set CWRW Box1 EC092 EC094 EC095 logger 3 Lo Alarm Setpoint Hi Alarm Setpoint Alarm s Triggered Calibration due Graph Start Graph Stop Signature 2 Print Name Date Page 1 of 1 40 0 C 85 0 C No Not Set 29 feb 2008 11 38 00 PM 6 mar 2008 9 00 00 AM MAX 80 307 MIN 13 160 RANGE 17 440 2 mar 08 3 mar 08 00 00 00 00 4 mar 08 00 00 5 mar 08 6 mar 08 00 00 00 00 CWRW Box2 EC096 EC097 EC098 Page 1 of 1 SL52T Standard print printed on fredag 16 mai 2008 at 13 54 09 Logger Serial No 66000000050EE941 Lo Alarm Setpoint 40 0 C Total readings since new 25676 Hi Alarm Setpoint 85 0 C Sample Rate 10 mins Alarm s Triggered No Logged readings 4096 Calibration due Not Set Issue Time Date 29 feb 2008 9 58 11 AM Graph Start 29 feb 2008 11 38 00 PM Graph Stop 6 mar 2008 7 00 00 PM Owner Manifest Not Set User Manifest 1 CWRW Box2 EC096 EC097 EC098 User Manifest 2 Logger 4 Signature 1 Signature 2 Print Name Print Name Date Date KERR MAX 30 83 C MIN 13 32 C RANGE 17 re dal d BEE MM MEANS 1884 C 30 0 C e I i 28 0 C
120. erbedingungs Nr DRL DRD No Gruppierung Dok Group Doc related Thema Subject Kurzbeschreibung Abstract AY LOR Autor S Li Org Einh Prepared by R Born Organ Unit TO 53 o G ft M c a Oo Einh epr ft rg Einh Agreed by T KL Organ Unit TO 53 Gepr ft Org Einh Agreed by daz bi amp ot Organ Unit TOQS Genehmigt FHN local ECB Chairman Org Einh g Einh Approved Organ Unit TE Te gt 29 BA Klassifikations Nr Classification No Freigabe Nr Release No Gruppierung Version Group Version related Unternehmen Company EADS Astrium Unternehmen Company EADS Astrium Unternehmen A Company EADS Astrium Unternehmen i Company EADS Astrium Dok Nr No EMCS RP 0000 149 DOR x Failure Analysis EADS jo en Report a Ausgabe lssue 1 Datum Date 1 8 2008 Seite Page 2 von of 5 Daten Dokument Anderungsnachweis Data Document Change Record DCR Ausgabe Betroffener Abschnitt Paragraph Seite Anderungsgrund Kurze Anderungsbeschreibung Issue Affected Section Paragraph Page Reason for Change Brief Description of Change first issue Table of Contents 1 INTRODUCTION oc E 3 2 REFERENCE DOCUMENTS Aineias ee 3 3 SUMMARY OF RESULTS iere terti teet eh ed pon and seit Ete Eget dane sin pede ume thane 3 4 REGOVERY nn EE R a a a ak te a ep n bek y 5 5 APPENDIX AS RUN PROCEDURE sese aa asa oss
121. ere collected in fill in tables in the procedures and are summarized in Table 2 CW RW PCC Assembly and Seed Set Up Report N USOC TR 027 Issue 1 Date 13 02 2008 Page 8 of 105 Table 2 Changes and variations to the procedure ADO4 Test step No Test step description Remarks 1 3 Zeolite filtration and enrichment was done prior to Multigen 1 experiment Same batch was used for this set up See N USOC PRO 002 Issue 1 3 Growth pots and growth pot lids were not marked Unlike for Multigen 1 the growth pots for CW RW Has the same configurations with 7 hole lids No need to use exsiccator because pot is left to dry in flow bench over night 10 Adjusted weight to 0g with growth pot integration tool and Petri dish 16 Used both integration tool stamp and small spatula to get felt under the edge 17 18 between Put in top o ring This step was not included in the procedure 19 There is no reference rod in CW RW lids The lid with borosilicate was pressed down on a piece of cardboard to get the borosilicate into the recession 29 After mounting the doughnut the seed position was checked 30 A small amount of silicone was applied on the mini lid male tread before it was put in the holes Lids were then screwed in until leveled with PCC lid surface Seed position was checked Better precision of silicone appliance and minimize smea
122. es continuous power during the entire 53 day experiment run but can withstand power interruptions for up to 5 hours during the first 43 days and up to 3 hours during the last 10 days of the experiment run Both Payload Anomalies and ISS Power limitations lead to periods of power interruptions For detailed descriptions of incidents of power interruptions and other interruptions to environmental stimuli contact N USOC Payload Anomalies Main Experirment objective By using Arabidopsis the goal is to identify gene sets which play a role in gravity dependant formation of supportive tissue in plants Therefore it is desirable to cultivate these mutant strains up to reproductive stage under microgravity and at 1 g conditions on orbit for comparison with the wild type When retrieving the plants to earth changes in expression of genes involved in formation of microtubules plasma membrane and cell walls can also be analyzed No plants developed up to reproductive stage Anomaly PAR HW 001 1 Payload Anomalies Temperature CWRW EIR Exec 08 Temperature inside the EMCS Experiment Containers during experiment execution shall be 2321 C On day 46 the temperature in the EMCS incubator was lowered to 20 C To reduce dehydration of the plant in B4 FM100 PI ESA Samfunnsforskning AS N USOG rt and Operations Cent Wes EMCS CW RW Requirements Integration Report Doc Nr N U
123. est of plants in B3 and B4 In total all ECs were removed from the EMCS Inability to hydrate led to very few plants to harvest Payload anomalies PAR EMCS HW 0011 Payload Anomalies and ESA PI agreement Doc Nr N USOC REQ 025 EMCS CW RW Requirements Integration Issue 1 Report Date 28 11 08 N USOG m3 j Norwegian User Support and Operations Centre GR Page 28 of 30 Samfunnsforskning AS 4 3 Main Reason for Failure The investigation of EMCS and CW RW hardware returned on 1J led to the final conclusion to the main reason for failure of the CW RW experiment ref Appendix 7 Failure Analysis On EMCS RBLSS MODULE FM008 the Quick Disconnects QDs towards fresh and waste water reservoir were swapped meaning QD 10 N Fresh at RBLSS was equipped with a female QD thus fitting into waste QD of the water Reservoir and vice versa QD 12 N Waste at RBLSS was equipped with a male QD thus fitting into fresh QD of the water Reservoir As it was confirmed by the supplier OHB the same discrepancy is most likely present on FM007 which was also used during CWRW and which is still mounted in the EMCS FM on rotor A Development of the failure Following the history of CWRW the following happened At the beginning of CWRW the humidity RH control of all ECs were started with 5096 setting Due to the QD swap no fresh water could be supplied to the humidifiers which was not immediately recogn
124. ests pink blocks represent set up for flight and orange blocks represent Real Time Operations Event September October November December January February March 07 07 07 07 08 08 08 Test 1 pH Test 2 Test 3 Test 4 Test 5 m Test 6 Test 7 TBD TBD Test 8 TBD RT Set Up Exp run on orbit Planned CW RW Tests I Flight preparations Experiment execution on orbit Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 Integration Report Date 28 11 N USOE 3 tegration Rep ate 28 11 08 Norwegian User Support and Operations Centre Page 1 of 1 Samfunnsforskning AS Appendix 2 Filled in PCC Assembly and Seed Set Up Report N USOC TR 027 CW RW Issue 1 PCC Assembly and Seed Set Up Report Date 13 02 2008 Page 1 of 105 Cell Wall Resist Wall PCC Assembly and Seed Set Up Report Date Prepared by Maja Rostad MEET Checked by Suzanne verlie REENEN A ae EE U EA P E P E E PA A E P EN Motoshi Kamada u u oiai meno aak an oanien aaea aaa aa ea aR Approved by Kazuhiko Nishitani coscienti ke Takayuki Hoson Hem TOU SAIMAZU m Rs Quality Assurance E Ne D N USOC Project Management All ROS SUNN TT EMCS PIM Ulrich WUD ON sme Document type Report CIRiS Center for Interdisciplinary Research in Space NTNU Samfunnsforskning Dragvoll Alle 38 N 74
125. et Z00 O ld DOSN N OQNLN soueunssy AWEND uone201 sjeg A0 PaUHONSd prasoy VOU Saa 29 19 HT 8919 X FAZ LOY QOd on oui Jod uoc eui BuipinB UeYM uorssea2es Jeng Jod YIMOIS eui UUM Juswu lle 1994102 U SI Nakrdq Su ens BYEN Buniesur USYM Buu o ejddiu 4ejem jjews y pue Guu o jog UIMOIS ay abewep 0 jou Lake eq Pinoys aes epads jog Jeuuj ay ojui SPIEMUMOP 1 Butysnd Aq jnjeJeo jog UIMOJS ay Junoyy uonejueuo umous au 0 Buipioo2e JIJ eui 0 30d YIMAL9 ayi uBIlV jd MOIE eui JO apisjno out uo Buu o a Jeypem eseauc wWnnoeA yH Addy l wopoq 99q eui ul pu y aoqe I Bujuado Jejnouo eu jo Bp jews y sapun peuuid aq pinous jer eur spueu p Ao B Bursn Aq 99d eui Jo ped uionoq au i ul J8J dd PSABpojne juewajdwij Jod UIMOJS ay JO epis no ayy uo AIAIAIATATAIATA I uorsseoal ui pe e oojne pue jno aJd Epbtidd Yer dd 19ursjdui alah la ta tat a oueyje uy anssy Jaded say snp UM y Budim Aq 3 ezi 1ojs aoepins pue uoueq MOL ajas aui olur 93 eui Bung ajddiu iayem uo pezijjeys Buu o jews 1u uu duuj Jh DEE 22d Jo Ajquiessy 7 7 p uuoli q yJewoy o oyd rousq uonduoseq deis 95 10 17 eed 2002 50 E2 EG CL enss ainpss0ig dfy jeg paas pue Ajquiassy 99d L ueBnnIy ZOD0 Otld DOSN N eougJnssy JJEND UopeJoj ag Aq pauuopad ab VAY Sd 8010 bT 9019 21 BS VONDE Sy JO 89Ju uo 0 LINZ 0 Ae uejy wuz e Buisn smauas ZLG III i NIG 9XZW Ano yo asn Aq 99d auj 0 JaA09 Du
126. fresh QD of the water Reservoir The function of pumps valves and performance of RH control were tested in the following sequence No Step nominal value actual value 1 Switch on and off all valves one after the other audible click click 2 Switch on and off air pumps one after the other flow rate at GSE PA1 0 38 l min 20 3 l min PA2 0 41 l min 3 Switch on and off all water pumps default direction one after rotation visible and rotation visible in the other in correct direction correct direction 4 Switch on RH control 5096 on pos 1 and 2 and wait until HU approaches to ok equilibrium is reached 50 DH approaches to 40 The filter housing F1 to F6 were inspected Filters look clean without degradation During all tests see chapter 4 it was found that the RBLSS Module is working completely nominally Therefore no further disassembly or investigations were made with the stand alone RBLSS The QD swap described above has dramatic consequences on all EC positions of each rotor it was confirmed by OHB that not only RBLSS FMOOS but also FM007 are concerned Due to this swap all positions are connected in an unfavourable way as shown in Figure 7 1b compared to the correct connection Figure 7 1a Y A1 A2 A3 A4 A1 hum A2 hum A3 hum A4 hum Figure 7 1a correct connection of water supply to EC and to from RH control A1 to A4 and the RH loops are supplied from fresh water bag drai
127. g dag 95 jo Gt eDeg 100z so ez aea SUEIQUSN VAd Jo uogezijuejs pue Bulpinoyy CL anss v xipueddy tO0dVW 200 OUd J0SN N eougJnssy JJENT uojeoo eg Aq pawoyed WON Sd SOIO ET SOUD hl WUD uen SBINUIW OZ JO 9 02 JE ezijw9 s pue eAe oojne ue ul Jef ssejB SU SJBl4 SOUBJQUISIN VAd 84 3M jogjuoo ul 185 o aAR 001NE y Ul seyem peje10deAe Aue piore oj Ajinjeleo pi USJUBIL Buyeas pue pip 3ueueyuindde yum sef ssef qu 005 episu usip Med pasop seld ede saepo ne Buisn Aq usip meg 01 PIT USIP Wed 4907 soejd ut Aeys wou syew oj SSUBJQUSN vAd 34 pue sjeeus esojnijevouu au uo aImnsseld Ajueae jnq pue Ajdde oj si aoed uogoo o JO esodund au usip meg aui oj uo prj usip 1494 eu Buryoeye 210189 uopoo eui uo J JEM Jo rl cz Ajddy euinjo4 USIP Weg eui Ley 19648 Jeymewos eq PINOYS ezis YSIP Weqg 84 OJUI J 0 U04409 Jo e eid e edeus Jeded Io esojnjjedojiu JO adA SLUES y JO Jsays MoU e YIM SSUBIQUSW YAd au 18409 xX1eulesj ojoug uoneJjsuouleg 95 jo gr 3fed sooz so ez eg eugJquio y vAd Jo uonezijuejs pue Hupo uonduosaq deis CL nssi y xipueddy v dV Z00 Otd 2OSf N S0UBINSSYV Alen Uogeoo JEG A0 pouwsoued Y N SIE K ID E 3010 hl SAU EE en gA anjea ienjoy eougJej o JEUIUON uondiuoseg dajs saj on days jsay a npsao d ayj oj suoneueA pue saDueu2 z alge G SIGE ui pejueuunoop eq Heys eunpeo2oJg 24 0 SUOHELBA pue seBueu
128. g eye irritation A response was immediately requested from the NASA JSC toxicologist The response received stated that if the PO can assure JSC Toxicology and the PSRP that dusts have been eliminated and essentially all Zeolite particles in the flight samples with larger than 0 5 mm diameter the Toxicity Hazard Level would be reduced to zero non hazard The Information contained herein is proprietary to PROTOTECH AS and Is to be used by the recipient solely for the purpose of which it was supplied It shall not be disclosed in whole or in part by any other party without the written permission af PROTOTECH AS prototec h EMCS Experiments Project 30193 Procedure for Filtering of Zeolite PARTICIPANTS REQUIRED 3 PARTICIPANTS REQUIRED Personnel required Engineer Responsible for e perform filtering according to procedure e filling out step by step procedure Quality Assurance Engineer Responsible for Issue Page B Date Doc no EMEX PR MULT 04 PRO Int Doc no PR 30193 PR 05 of 19 surveillance of the filtering and that filtering is performed according to procedure Responsibilit Table 3 1 Participants b Mrs Marian N Melle Y Ber d Ge c veia c r Geir Omdal 03 05 2007 Doc no EMEX PR MULT 04 PRO prototec h ga EMCS Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007
129. g the plants to earth changes in expression of genes involved in formation of microtubules plasma membrane and cell walls could also be analyzed Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 N USOC Integration Report Date 28 11 08 Norwegian User Support and Operations Centre IS Page 9 of 30 Samfunnsforskning AS 2 2 Experiment Unique Equipment The CW RW Experiment Unique Equipment EUE consisted of a Plant Cultivation Chamber PCC ADO7 which is an advanced flower pot that monitors the humidity in the growth medium and adds water when the delta pressure in the growth media is below a defined level A reference sheet was located on the base plate of the Experiment Container EC Figure 2 1 Figure 2 1 CW RW A Plant Cultivation Chamber PCC and Reference Sheet assembled on an Experiment Container EC Base plate B EC with EC Cover The growth support consisted of Zeolite ZeoPro with propylene felt PP felt on the top 2 3 CW RW Operational Scenario The CW RW Operational Timeline as planned before experiment start is outlined in figure 2 2 The CW RW pre flight operations and experiment as performed is documented in chapters 3 3 and 3 4 All samples were mounted on ground and the crew operations on board involved EMCS generic Crew Procedures EMCS EC replace EMCS Water Reservoir replace EMCS Valve movements EMCS Power Switch configuration and Clean Up activities post experiment and CW RW specific
130. hall be performed before the next experiment after CWRW Obs target and freq EIR Exec 09 1 Power cycle of EMCS SPLC VPU when VPU was locked in a busy state 2 EMCS schedule to check and repair VPU lockups A workaround to reset the VPU by ground commanding was developed during MULTIGEN 1 and improved during CWRW 1 Resume VPU capability period with loss of environmental control 2 Losing images during VPU lockup no loss of environmental control Gravity level EIR Exec 13 Illumination EIR Exec 15 Power interruptions EIR Exec 31 Obs target and freq EIR Exec 09 EMCS SW 0007 Unexpected Shutdown of EMCS Subsystems The TCS was powered down by the SPLC because the SPLC did not receive TM from the TCS for 30 sec All other subsystems were powered off simultaneously with the TCS as expected in such a case The other subsystems sent nominal telemetry and the EMCS power draw was nominal until the power down i e the TCS was still running nominally while no TM was received Impact on experiments loss of illumination and artificial gravity for a short timeframe Gravity level EIR Exec 13 Illumination EIR Exec 15 The subsystems could be nominally restarted after the incident 1 Resumed environmental control and images Environmental control not effected Gravity level EIR Exec 13 Illumination EIR Exec 15 Power interruptions EIR Exec 31 Obs target and freq EIR Exec 09 EMCS S
131. he air supply filter was dirty from air pump dust Figure 5 3 Figure 5 1 Figure 5 2 Figure 5 3 The flat sealing was not removed and after inspection the PEEK and bottom plate were mounted again 5 2 EC FM092 5 2 1 Before opening bottom plate Before opening the bottom plate the water interfaces were inspected The septa were protruding for 0 4 mm on both interfaces 5 2 2 Bottom plate and PEEK plate removed performed acc to EMCS MA 4000 004 DOR issue 2 sections 4 1 and 6 After removal of bottom plate the septum holder of supply I F was removed and inspected The inner side was partly filled with water Figure 5 4 which means that water passed the rotor I F and the septum towards EC Figure 5 5 After removal of the PEEK plate the filter assembly was found to be correctly installed The water supply filter upper side outlet towards PCC was wet Figure 5 6 Some water could also be seen on the PEEK plate Figure 5 5 and in the EC inlet hole Figure 5 7 However the channel towards PCC as well as the drill hole between septum and filter assembly was dry The channel could be dried by air which shares this way after passing the filter The air supply filter was dirty from air pump dust Figure 5 3 Jd Dok Nr No EMCS RP 0000 151 DOR a Trouble Shooting for EA D S me d SPR 283 Ausgabe lssue 1 Datum Date 18 8 2008 gSEr urm Seite Page 14 von of 24 Figure 5 5
132. i 0 pejosuuoo S AIBA Z UE ut S AJEA Buiyay ms ajiym Ajasojo pasojiuou eq pinous meup juano SY J UA papsemyoj SEM sanba e o pepuguiuioo USYM BUIYDNMS Ajjenjoe aie S MLA auj JI Ajilan 0 JepJo U LLO MH7HVd SOLLND SOLLND day Id Qd uonebnse u BINH NDE 4 JO Hed sas nd mojs pajuawajdu Z sasind pide 67 uleBe ajnpayos uoneJp u jeniur VEIS LLO MH7HVd SOLLWO SOLLWSO uoneBnseauj sine Aie Jo Hed Sand uoee ueewjeq pejueuiejdujj S A EA YIjIMS jou OG uoneAnoe dwnd uoee ues wjeq yem s lnului G ym Sel S uiu Ly ui dund Jayem uny uoneBnseAu eunjie4 le JO Yed SBemo s LI ae SIIOAJOSSY 187e M YUM JO uoneouyueA uuojied o meuo Joj JIAHIY moun sanbay day ld Duo paye s psuuejd se 9 y jo peajsul Aep aney yng Jejem u6noue 396 jou op Aay se y ur SBuljpaas jo jnoj Aana sunou G py OJ uoneurunili peonpey puewwog LLO MH UVd SOLLND pajuawsjdwij e e Er 9 LLO MH AVd POL p lu uu duu up pioAe 0 1 pio u epew si Aep Jed 1UBI sinou g Ajuo jo ysanba ayi il V O3 u pe1e oosip asam sBull p s sjdwaye uonelpAy sej Gung dey ld 6 o101W u UMOJB aq pjnoo YDIYM g Jojo uo sjuejd sidwaye uoneupAu Gxg Jaye v 10103 Jo uonejo doe OU eye 3484 USYM J0101 si uo 6x uni 0 esues ayew jou seop ji LLO MH UVd vOLLINO pajuawajdwij pue jueuioui SY Je Jojou y SY uo are sBui pees pajeuiuueb juo ayi day ld Dom UI UMOJ eq pjnoo YDIYM g 10101 uo sjuejd sjdwane uone p u Bxg 0 Jeye v Jojoy Jo uonejo doe OU aJe 8184
133. ing 5 S 2 1300 e o 1100 6 900 4 700 2 500 T T T 0 0 10 20 30 40 50 60 Number of 15 second pulses Figure 4 8 Result of water supply test with PCC number of pulses until p sensor increase 5 EXPERIMENT CONTAINERS 5 1 EC FM099 5 1 1 Before opening bottom plate Before opening the bottom plate the water interfaces were inspected The septa were protruding for 0 4 mm on both interfaces 5 1 2 Bottom plate and PEEK plate removed performed acc to EMCS MA 4000 004 DOR issue 2 sections 4 1 and 6 After removal of bottom plate Figure 5 1 the septum holder of supply UE was removed and inspected The inner side was wet some droplets on the surface which means that water passed the rotor I F and the septum towards EC Figure 5 2 After removal of the PEEK plate the filter assembly was found to be correctly installed The water supply filter upper side outlet towards PCC was wet Some water could also be seen on EADS _ astErium Trouble Shooting for SPR 283 Dok Nr No Ausgabe lssue Seite Page EMCS RP 0000 151 DOR 1 18 Datum Date von of 18 8 2008 24 the PEEK plate Figure 5 3 However the channel towards PCC as well as the drill hole between septum and filter assembly was dry The channel could be dried by air which shares this way after passing the filter or it was never wetted In any case it indicates that only a small amount of water 1ml entered the EC T
134. ing Transport N US0C Ws A Em and Check Out Procedure Recents Buer Lugnort und Baerabess Canio N Annex 3 Phytosanitary Certificate for Arabidopsis Thaliana Seeds One combined certificate for Wild Type GM GUS mutant lefty and mutant hmg N USOC PRO 005 mmm m CW RW Flight Sample Hardware Packing Transport issue 2 a and Check Out Procedure Date 27 02 2008 Page 43 of 58 PHYTOSANITARY CERTIFICATE PLANT PROTECTION SERVICE MINISTRY OF AGRICULTURE FORESTRY AND FISHERIES JAPANESE GOVERNMENT TO PLANT PROTECTION ORGANIZATION S OF Kingdom of Norway No 250072117 I DESCRIPTION OF CONSIGNMENT 1 Name and address of exporter 2 Declarcd name and address of consignee Suzanne Overlie The Plant Biocenter NTNU N 7491 Norway Toru Shimaszu 2 20 11 Midori Nishitokyo shi Tokyo JAPAN 4 Distinguishing marks NONE 3 Number and description of packages I Case 7 Declared point of entry Trondheim Norway 6 Declared means of conveyance Hand Baggage 5 Place of origin Japan 9 Botanical name of plants Arabidopsis thaliana B Name of produce and quantity declared 0 4 g Arabidopsis Seeds l Arabidopsis thaliana Columbia wild type 2 Arabidopsis thaliana Columbia lefty mutant 3 Arabidopsis thaliana Columbia lung mutant 4 Arabidopsis thaliana Columbia Transgenic promoter GUS This is to certify that the plan
135. inpadold dags Aq days ubis pue 3no jy bes UONGLDSOG dSIS 6T 140 tT 13684 Jiunaioo id dALS Ad diis eajosz JO GULIBJ IL 104 eynpe204g EGTOE od LZOOZ SO ED alBeg g anss SO Hd EGTOE Ud ou 200 201 SJUDLUMD XJ SOWA Otd P LINW Wd X33 ou 20g u223030Jd MCS Doc no EMEX PR MULT 04 PRO JJ E Experiments protote C h P Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 ACCEPT REJECT CRITERIA Page 15 of 19 7 ACCEPT REJECT CRITERIA The filtering is considered as successful if filtering has been performed according to procedure and no deviations anomalies have occurred 8 DEVIATION All deviations to this procedure shall be recorded on the Procedure Deviation Sheet in Appendix A 9 NONCONFORMANCES All Non Conformance shall be recorded in the Non Conformance List in Appendix B 10 PROCEDURE SIGN OFF SHEET Statement that the Zeolite has been filtered in accordance with the approved procedure to be signed and dated by Engineer Quality Assurance Representative and Customer Representative where applicable in Appendix C Doc no EMEX PR MULT 04 PRO prototec h p EMCS Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 TABLE LIST Page 16 of 19 11 TABLE LIST Table 2 1 Reference Documents E
136. is plant products or other regulated anicles described herein have been inspected and or tested according to appropriate official procedures and are epum to be free from the quarantine pests specified by the importing contracting party and to conform with the current phytosanitary requirements of the import ing y includi 1 H ADDITIONAL DECLARATION Ill DISINFESTATION AND OR DISINFECTION TREATMENT 10 Date TH Treatment XXXXX XXXXX 13 Duration and temperature 14 Concentration 15 Additional information XXXXX 16 Place of issue 18 Name of authorized officer Yokohama Plant Protection Station Narita Japan Masaharu Kojima January 20th 2008 No financial Tability ik respect to this certificate shall attach to the Ministry of Agncullute Forestry and Fisheries Japan of to any of ils officers or representatives N USOC PRO 005 CW RW Flight Sample Hardware Packing Transport issue 2 and Check Out Procedure Date 27 02 2008 Page 44 of 58 Annex 4 Verification 2 form N USOC PRO 005 N USOE TEE CW RW Flight Sample Hardware Packing Transport issue 2 Nerwteias Bits Ieren and oersass Cis UV d s and Check Out Procedure Date 27 02 2008 Page 45 of 58 To Froa Astriua ST Fan 49 7595 U 49 7545 LTOCALL at 00 01 25 17 35 Doc 190 Page 001 JSC Toxicology Group Environmental Faclors Branch John T James Ph D Toxicology Lead NASA Johnson Space Center SF
137. is thaliana The variation is Columbia Col O The seed stock was harvested October 15 2007 Production number is H 2007 02 The seeds are untreated The seeds will be received by Motoshi KAMADA JAXA Regards Mr Motoshi KAMADA JAXA 2 1 1 Sengen Tsukuba shi Ibaraki JAPAN 305 8505 Phone 8 1 29 868 3698 Fax 81 29 868 3956 e mail kamada motoshi jaxa jp CW RW Flight Sample Hardware Packing Transport and Check Out Procedure N USOC PRO 005 Issue 2 Date 27 02 2008 Page 41 of 58 Osaka city University 3 3 138 Sugimoto January 18 2008 Sumisyoshi ku Osaka JAPAN 558 8585 Phone 81 66605 2577 Fax 81 66605 2577 The following information is submitted in support of shipment of seeds by Norwegian University of Science amp Technology NTNU The name of supplier is Professor Takayuki HOSON Osaka city univ Osaka Japan The name of contact person for supplier is Professor Takayuki HOSON The name of the seed is Arabidopsis thaliana The variation is Columbia Col 0 The seed stock was harvested October 15 2007 Production number is L 2007 02 The seeds are untreated The seeds will be received by Motoshi KAMADA JAXA Regards dora Mr Motoshi KAMADA JAXA 2 1 1 Sengen Tsukuba shi Ibaraki JAPAN 305 8505 Phone 8 1 29 868 3698 Fax 81 29 868 3956 e mail kamada motoshi jaxa jp N USOC PRO 005 Issue 2 Date 27 02 2008 Page 42 of 58 CW RW Flight Sample Hardware Pack
138. ivity Working Group with participants from ESA IOT PI and N USOC as described per AD11 Test Procedures were developed by N USOC and reviewed and signed by participants of the EMCS GM Activity Working Group Test reports were prepared by N USOC after test finalization and the reports were reviewed and signed by the same participants of the EMCS GM Activity Working Group Doc Nr N USOC REQ 025 Samfunnsforskning AS EMCS CW RW Requirements Issue 1 Integration Report Date 28 11 08 N USOG m3 a d Norwegian User Support and Operations Centre Page 12 of 30 Table 3 2 The table summarizes CW RW tests performed in the EMCS Ground Models test facility test objective start and end date and test report number Test Test objective Facility Test Objective Start End Report 1 Science Test ERM The Arabidopsis thaliana seeds from wild type September November N mutants and GUS GM should germinate and 19 7 USOC develop 10 cm high stems within 50 days TR 28 Criteria 1 Germination Rate Group 1 Wild Wild GM and Mutant lefty Average 80 of seeds 7 seeds of total 9 seeds PCC Group 2 Mutant hmg Average 50 of seeds 3 seeds of total 6 seeds PCC Criteria 2 Stem evolution Rate Group 1 Wild Wild GM and Mutant lefty Average around 60 of Mini Lid 3 stems of 5 Lid PCC Group 2 Mutant hmg Average around 50 of Mini Lid 1 stems of 2 Lid PCC 2 Recycling Test EM Optimize the CW RW watering
139. ized because they contain sufficient water to supply 5096 humidity for quite a long time However the wrong connection led to an accumulation of an air water mixture 9096 air 1096 water volume in the fresh water reservoir instead of waste because the dehumidifier is drained every 3 hours n parallel to RH control the initial watering of EC PCC was commanded starting with positions A1 A2 B1 B2 These positions now tried to get water from fresh reservoir but in fact were connected to waste reservoir which nominally did not contain free water at the beginning of an experiment So these positions only received the small amount of 1 5 ml water which was contained in the tubes Initial watering of positions A3 A4 B3 B4 was started several days later Since these positions were connected correctly they tried to get water from fresh reservoir but received only an air water mixture which was partly sufficient for triggering the p sensor in the PCC However on a long term since the trapped air in the fresh water reservoir increased by the RH control the supply could not be supported sufficiently and the plants dried out After the unsuccessful trial of seven ECs the water reservoirs were exchanged Before the water reservoir exchange no position could be hydrated at all This can be quantitatively proven because the watering of A3 A4 B3 B4 positions followed much later than the 1 2 positions After the exchange there was a partial
140. jautomaticcaly at 100 pulses 75 pulses 1 Rotor A to fill tubes from H20 to RBLSS with water 3 min Pump1 1 min pump 2 100 pulses sch stopped automatically activity EMCS Water Flow Checks H20 to RBLSS with water 3 min Pump1 1 Drained de humidifier to Waste 3 min Power down for crew activity EMCS Water Flow Checks 60 sec Patches water pulses back to 15 sec Normal hydration sch 92 pulses Resat counter to 30 91 30 61 pulses Resat counter to 30 64 30 34 pulses Resat counter to 0 Patches water pulses to 60 sec sec Counter set to 25 to avoid rapid pulsing Hydration performed in AOS only Sch stopped automaticcaly at 100 pulses 75 pulses given Patches water pulses back to 15 sec Patches water pulses back Patches water pulses Power down for crew activity EMCS Water Flow Checks Patches water pulses Pumps water from fresh to waste in 1xg on Box 1 Rotor B to fill tubes from H2O to RBLSS with water 3 min Pump1 1 min pump 2 started 100 pulses given Sch stopped automaticall started 100 pulses given Sch stopped dration in Oxg started Hydration in 0 8xg started 100 100 pulses given Sch pulses given Sch stopped stopped automaticall automatically dration in 0 8xg started 93 pulses given Sch stopped automatically ydration in 0 8xg started 93 pulses given Sch stopped automaticall ydration in 0 8xg started 73 pulses given Sch stopped manually at this point
141. jective of germination and growth in 56 PCC Lid holes was not met Many of the CW RW experiment requirements were initially broken due to Payload and EUE anomalies and limitations The investigation of EMCS and CW RW hardware returned on 1J led to the final conclusion to the main reason for failure of the CW RW experiment On EMCS RBLSS MODULE FM008 the Quick Disconnects QDs towards fresh and waste water reservoir were swapped As it was confirmed by the supplier OHB the same discrepancy is most likely present on FM007 which was also used during CWRW As an attempt to optimize the remainders of the experiment the PI team and ESA agreed upon changes to the original experiment requirements In order to implement these changes several actions were taken which had been tested on ground only to a limited degree On some of these occasions implementation of the changed requirements were tested and advised against but led to unfortunate consequences like flooding of the EC B3 bottom chamber which in turn led to the seedlings in this position to perish All in all the Cell Wall Resist Wall Experiment unfortunately failed in spite of all the time and effort that was dedicated to making it a success The reason for this was most likely due to two faulty mounted EMCS RBLSS Modules Doc Nr N USOC REQ 025 EMCS CW RW Requirements Integration Issue 1 Report Date 28 11 08 N USOG P Norwegian User Support and Operations Centre Page 30 of 30 Sa
142. jenuew Woe sas nd CL sas nd GXp aomas duund Jsjem Aq pg Jo UONEIP Y WIOHSd sasind pg jo uonelpAy jsanbay sasind vg jo uoneJpAu jsenbay sasind c pg jo uonelpAy 1sanbay yuidn aj 10 jsenbay yurjdn aj 1oj senbay sebueyo enpeoold MUMO CLIP AWANOY NVA Joj uonisod ISULID JO UONduasaq 12 Oa Bunee N ssauipeay youne snqunjog E LO MH SOW3 SPLINO EPLLWO Eb LLIND day Id si Aq paseasou si syuejd juetuuedxe ay jo LLO MH SOW3 pajuawajdwj ayi Jo jno up jeuuny jo ysu eui u6nou USAS pejeAnoe eq Pinoys 1se1 eui 104 snonunuoo peonpas uo uoneurunii daay pue 1u uudo A p juejd Jayyny enuajod Jo yueuoduui si uonguluunijj HOISSILuGns C uonisod AVA PILPY CHILO TS yep ang 10 enm 10 gUIBLIO SUEI 10 UOSEIN ISUBI JO UONdLNsag JOE 94 YL JO 4 eBeg ISI Way uoo Sunaap ssaujpeay youne snquwnjog Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 Integration Report Date 28 11 N USOC B J g p ate 28 11 08 Norwegian User Support and Operations Centre 7 Page 1 of 1 Samfunnsforskning AS Appendix 7 Failure Analysis Report SPR 283 r Failure Analysis D J Dok Nr No EMCS RP 0000 149 DOR E R eport SPR 283 Ausgabe Issue 1 Datum Date 1 8 2008 m t um H Seite Page 1 von of 5 D Failure Analysis and Recovery Report SPR 0283 Konfigurations Dokumenten Typ Nr Document Type Configuration Item No Referenz Nr Reference No Lief
143. karatqtra iet Sepoaet sag boprssses Csin ji w GW AW Test Author e Suzanne verlie Checked by Signature Ann lren Kittang Knut Fossum Motoshi Kamada Ulrich Kuebler Wilfried Biemann Thomas Niedermaier Signature Approved by Toru Shimazu Reason for Change CW RW Seed Set Up Procedure vU 5 GW RW Test Procedure xus OWIRW Seed Set Up E Signature PU STE N USOC PRO 004 Issue Issue 1 Date 18 01 2008 Page 2 of 13 Affiliation Dater oo N USOC Affiliation Date N USOC N USOC Manager JAXA CW RW Team Coordinator ESA EMCS CW RW PIM Astrium IOT PA Astrium Affiliation Date JSF PI Rep CW RW Seed Set Up Procedure Nermtgtes Put Supert nas Operations Cartre n t V 1 Applicable Documents 2 List of Abbreviations u s w Aw aw unsern 3 Scope of Document LU LLL oe rei s kous EENEG sc era Ua 4 Differences from Multigen 1 Set Up Procedure e b OO GE n n e an ok E 0 YE Test Procedure Variation Fill in Procedure Sign Off cssseccsssesessesesseessecessessecesserensertenseteenaees ou N USOC PRO 004 Issue Issue 1 Date 16 01 2008 Page 3 of 13 OTTELE s NTN U N USOC PRO 004 Social Research Ltd CW RW Seed Set Up Issue Issue 1 N U50G Mara Procedure Date 16 01 2008 oam tt nsyon und Promien Comte Page 4 of 13 1 Applicable Docume
144. le 3 1 For further details on scientific status after execution of the experiment reference is made to reports made by the ESA Mission Science Office ESA MSO Team and the Principal Investigator Team Table 3 1 CWRW Integration and Real Time Operations Flow with time aspects responsible parties and resulting products Consolidation Integration Start End Responsible Product and Real Time Ops Requirements Consolidation N USOC Consolidation List phase Integration Requirements 29 03 07 N USOC EIRD Document CW RW Test Campaign 17 09 07 09 01 08 Test Reports Requirement input to NASA N USOC URC ground rules amp POIC Constraints Payload Regulations Experiment Sequence Test 19 02 08 21 02 08 N USOC EST Report EST Flight Sample Set Up 15 01 08 21 01 08 Pl Flight samples Current Report EC Check out 21 01 08 25 01 08 IOT HFIT IPLAT Verified ECs Report by IOT HFIT IPLAT Transport Handover 03 03 08 10 03 08 N USOC Samples handed over to NASA Current Report Real Time Operations 29 03 08 23 05 08 N USOC Current Report CW RW Change Request Log OSRs 3 1 CW RW Integration Testing in EMCS Ground Models As part of the Experiment Integration 4 tests were performed in the EMCS Ground Models at N USOC A summary of these tests can be seen in table 3 2 All activities involving usage of the EMCS ground models were coordinated within the EMCS GM Act
145. list and IOT insurance COV STAG PO EE NEN FUA MARE Fe ne ann so annona poo sea tta rabo TREE Er Kan Ne ta vae sans noon eher 52 N USOC PRO 005 N US0C rm CW RW Flight Sample Hardware Packing Transport issue 2 Nenstelzs User Lesoori and Bperstians Lasiry gi and Check Out Procedure Date 27 02 2008 Page 4 of 58 1 Applicable Documents Applicable Documents AD Document Number We 7 uberes ADO1 EMCS MA 4000 002 DCR 12 11 2004 EC User Handbook AD02 N USOC PRO 002 20 06 2007 Multigen 1 PCC Assembly and Seed Set Up Procedure ADO3 N USOC PRO 004 16 01 2008 CW RW Seed Set Up Procedure ADO4 N USOC TR 027 13 02 2008 1 Cell Wall Resist Wall PCC Assembly and Seed Set Up Report ADOS EMCS RP 4000 033 DOR 31 01 2008 CWRW EC Checkout and Inspection Report Final Flight Preprations N USOC PRO 005 e CW RW Flight Sample Hardware Packing Transport issue 2 and Check Out Procedure Date 27 02 2008 Page 5 of 58 2 List of Abbreviations EC Experiment Container EM EMCS Engineering Model EMCS European Modular Cultivation System PI Principal Investigator PA Product Assurance QA Quality Assurance ESA European Space Agency NASA National Aeronautics and Space Administration IOT Industry Operator Team N USOC Norwegian User Support and Operations Centre KSC Kennedy Space Center N USOC PRO 005 CW RW Flight Sample Hardware Packing Transport issue 2 W and Check Out Pr
146. lse behavior as expected no delta pressure no water not considering offset delta pressure when connecting the QDs spring loaded volume change EADS E rL im Trouble Shooting for SPR 283 Dok Nr No EMCS RP 0000 151 DOR Ausgabe lssue 1 Datum Date 18 8 2008 Seite Page 5 von of 24 Note Contrary to this Figure these QDs are swapped in FM 0007 and FM0008 V1 is connected to ZE not fresh Fresh Water i 03 EC out Figure 4 2 Circuit Diagram EMCS RBLSS aste Liquid RBLSS FM diagram 1 dsf 03 07 2001 Nu di si a S11 Eee 02 mier Fresi Wast RBLSS Box 1 5 N N b nib l L Ji EC Position 2 BON s EN Na p EC Position 1 VG V5 PW2 v4 ET PWI v2 v3 02 um Filters E 0 2 um Filters 4 V 7 v8 P N L Nick PW3 amp le e N a d d HH Hos MA Hat hp 07 QD 04 x QD 03 QD02 I I QD 01 QD
147. mfunnsforskning AS Appendices Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 Integration Report Date 28 11 N USOC B a g p ate 28 11 08 Norwegian User Support and Operations Centre Page 1 of 1 Samfunnsforskning AS Appendix 1 Cell Wall Resist Wall Test Plan NTNU e Norwegian University of Science and Technology N USOC Norwegian User Seppori and Dperaligas Ceatre an In AN CW RW Test Plan N USOC NOT SEO 001 Issue 1 Date 09 01 2008 Page 1 of 2 CW RW Test Plan Table 1 The table summarizes planned CW RW tests in the EMCS Ground Models with details on test objectives test facility comments resources needed start and end date test manager and outcome Test Test Facility Comment HW S W Start End Test Man Status objective and amp resources Assistance 1 Science Test ERM Verify the duration time of the experiment When stems have reached height Schedules September November S E O of 10 cm Approximately 50 days Test 19 5 H S Procedure 2 M ECs 2 Recycling EM Transfer mature plants from ERM after the ST to the EM Verify the ability of Schedules November November A G Test the EM to support growth of mature plants PAR HW 0006 Dehumidifier Test 5 14 G A issues Procedure 2 ST M ECs 3 Germination EM When will recycling start after the initial watering Around day seven Switch Schedules De
148. mipaw Booys abiyseinyy uy G 0 e oqe ezis aiod uy ejjoez youug UJPIM USSLU LULU g0 YjIM aaais yno uieBe azs eJod uiu g mojeg LONDEA 9111097 194 U pIM us uu ww g uj ASS YGNOJYI 8040 81037 Jayi peuuogiag x1eurasojoug oureq se ojb jnoujm Deg ent sdejs y Buipnjoui se o B uj peuuoned eq pinous sdejs Iv ejoN Uonduosap dejs Aq deg jusuiiedx3 p e qe y 2 92 ui sdejs oun a Burpioooe peuuoped si dn jas 99d 8u L sdejs a npa90Ad L 98 JO L L beg 4002 90 ez eiea CL anssi eanpeooJg dN I2S pees pue Ajquassy 32d L ueBniniy 31184 pit cranii 200 ONd DOSN N saoueinssy NEND Pas 2h gono gr ww std SONG v spade 8010 21 vo s ode ey we steil bbO PUYSLOW4 SDA PALUNDM MAG Lon oo PUY SLO ELO pO hbO TWA 593 sof abuods parnou Maen Powola 95 jo zL abed 4002 SU ez ee SO IDET B010 p x1euioM ojoug ouiag eg Aq pauuojsg 3 9 de vpowoy HUSJON S due NG VAM dic LapuasoA Jowugkr Ap Ajayajdwios si eBuods eu nun 14BIU JEAO uoueq MO 9Juejs ui jog YAOI YM aseg joo vonelbajuj SARS aseg 001 uoneJBeju y JO LIONOG ay Jsulebe Ajwuy eBuods sy sseid o Jep o Ul smelos eseg jooj uone 6ayu um eseg jooj vonefssjuj oun oj ApnuBn pexoo eq jsnw 10d UIMOIS SYL Jam Us SI eBuods e iuw euop sq snu SIU I Youaq MOL Steis eui episui eseg joo uomneJ8ejuj au o1 30d UIMOJS SUD YPEY
149. ms so they can grow in an upright position Different types of cell wall components such as cellulose xyloglucan and lignin are all involved in wall dynamics which give this type of support to plant tissue It is expected that the metabolism and gene expression of a number of these substances is highly sensitive to gravity And one wished therefore to examine the gene expression of these cell wall related gene families in a micro gravity environment By using Arabidopsis Thaliana the goal was to identify gene sets which play a role in gravity dependant formation of supportive tissue in plants Resist wall Plants have two major responses to gravity roots experience gravitropism they grow downwards and the shoot experiences resistance to the gravitational force by growing upwards Little information about resistance to the gravitational force has been obtained compared to gravitropism The hypothesis is that the mutual dependency between microtubules the plasma membrane and the cell wall in plants is responsible for gravity resistance Arabidopsis mutants that are defective in various ways of forming these cellular components show distorted growth on earth However it was expected that such mutants are rescued and can grow and develop normally as wild types under microgravity in space Therefore it was desirable to cultivate these mutant strains under microgravity and at 1 g conditions on orbit for comparison with the wild type When retrievin
150. nature Affiliation Date Toru Shimazu JSF Pl Rep Change Log Reason for Change N USOC PRO 004 CW RW Seed Set Up Issue Issue 1 Procedure Date 16 01 2008 Page 2 of 13 Approval Title CW RW Test Procedure Issue Draft CW RW Seed Set Up Author Signature Affiliation Date Suzanne verlie N USOC Checked by Signature Affiliation Date Ann lren Kittang N USOC Knut Fossum N USOC Manager Motoshi Kamada JAXA CW RW Team Coordinator Ulrich Kuebler ESA EMCS CW RW PIM Wilfried Biemann Astrium IOT PA Thomas Niedermaier Astrium Approved by Signature Affiliation Date Toru Shimazu Z JSF PI Rep 16 Jan 2008 Date NTNU Social Research Ltd N USOE Borat pan bytt Sreser 228 tarratyas texte Title Author Suzanne verlie Checked by Ann lren Kittang Knut Fossum Motoshi Kamada Ulrich Kuebler Wilfried Biemann Thomas Niedermaier Approved by Toru Shimazu Reason for Change CW RW Seed Set Up Procedure N USOC PRO 004 Issue Issue 1 Date 16 01 2008 A CW RW Test Procedure CW RW Seed Set Up Signature Signature To Nest gt Signature Change Log Page 2 of 13 roval Issue Draft Affiliation Date N USOC Affiliation Date N USOC N USOC Manager JAXA CW RW Team Coordinator ESA EMCS CW RW PIM Astrium IOT PA Astrium WI BA D se Affiliation Date JSF PI Rep Date amp NTNU Social Hesearch Lid usor Nm
151. ned V 2 form needs to be telurmed Contact Ihe toxico ogist if you have any questions V 2 for STS 123 TEST MATERIAL CHEMICAL DATA VERIFICATION STEP 2 Please check one box below and fill in the blanks DI The materials loaded are exactly as listed in the verified V 1 HMST for this payload K The materials loaded are consistent with the PSRP policy stated above deletions corections are marked on Ihe attached verified V 1 HMS or this payload A Signature a OE Ze Date _22 E L en Print Name Hete ski KAMADA _ Organization JAZA EET e Experiment Acronym Name Ci Zu EH A Role associated with the Payload Experiment S ere gon dala Telephone tP 2 H NAN S Fax y x KI Hir N USOC PRO 005 o a o c S v o lt x n 2 G 3 B G I 2 a E G O 9 D Oo NM N USOG 27 02 2008 Date and Check Out Procedure teias Beer Lennart and Geerations Casiri Page 46 of 58 Verification 5tatus STS 123 1JA Nerifieation 1 Status HAZARDOUS MATERIALS SUMMARY TABLE HMST V 1 Data Verified by P I To ISS JEM V 2 Expt Name Cell Wall Resist Wall European Modular Cultivation System Expi Heber Acronym CWRW EMCS HMST Record d Part ris Maximum Volume or Amount lety Level Record update history Contact Person Comments Maximum OpNom Concentration mability Tome Hazards vx Haz Level
152. ned water from RH loops is collected in the waste compartment Waste is Dok Nr No EMCS RP 0000 151 DOR Trouble Shooting for EADS SPR 283 Ausgabe Issue 1 Datum Date 18 8 2008 a3Sskcriumnm Seite Page 23 von of 24 A1 A2 A3 A4 A1 hum A2 hum Air water A3 hum A4 hum air Waste Figure 7 1b Wrong connection after QD swap From positions A1 A2 RH loops an air water mixture but mainly air is pumped into the fresh water bag this air water mixture is then supplied to the correctly connected positions A3 A4 Supply to A1 A2 and RH loops is connected to waste compartment which does not contain free water 7 2 Correct RBLSS QDs by swapping Fresh and Waste a The QDs of FM 007 were exchanged to correct positions according to RD8 RBLSS FM007 still in orbit is also affected and shall be repaired b It was found that the thread of the male QD of FM008 was slightly worn Therefore this QD shall be replaced by a new one 8 INSPECTION OF MULTIGEN EXPERIMENT CONTAINERS Inspections of the Multigen Experiment Containers from the previous EMCS mission can support trouble shooting for the CWRW ECs as the latter are electrically identical and mechanically only deviate in a few holes in the PCC lid i e water and air channels are the same All 8 MULTIGEN ECs were hydrated thus serving as a successful reference Four of these ECs FM055 to FM058 were returned to Astrium During MU
153. not be disclosed in whole or in part by any other party without the written permission of PROTOTECH AS Doc no EMEX PR MULT 04 PRO protote C h 4 SE Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 Description Page 9 of 19 5 DESCRIPTION Filtering of Zeolite No particles below 0 5mm in diameter 5 1 Equipment Filter jig FJ Filter jig with Propyltex PP felt with mesh opening of 0 5mmx0 5mm SEFAR PP Felt article no 05 500 36 Container 1 C1 One container same size or bigger than Filter jig collecting the Zeolite particles below 0 5mm in diameter Container 2 C2 One container or glass for collecting the filtered Zeolite 5 2 Filtering of Zeolite 1 Place C1 on a clean table and place FJ on top of C1 As shown in picture below s proprietary to PROTOTECH AS and is to be used by the recipient solely for the purpose of which without the written permission of PROTOTECH AS Doc no EMEX PR MULT 04 PRO prototech IP EMCS Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 Description Page 10 of 19 2 Pour unfiltered Zeolite into FJ do not filter to much Zeolite at a time See pictures below The information contained herein is proprietary to PROTOTECH AS and is to be used by th
154. nsor in the PCC However on a long term since the trapped air in the fresh water reservoir increased by the RH control the supply could not be supported sufficiently and the plants dried out e After the unsuccessful trial of seven ECs the water reservoirs were exchanged Before the water reservoir exchange no position could be hydrated at all This can be quantitatively proven because the watering of A3 A4 B3 B4 positions followed much later than the 1 2 positions e After the exchange there was a partial success in watering B1 B3 B4 Since B3 B4 watering followed immediately the water reservoir exchange only some air was collected in the fresh water reservoir and the positions could receive some water B1 did not show a proper triggering but a kind of partial triggering which is explained by a mixture of air with some water which led to this behaviour A4 was also watered quite soon after water reservoir exchange but did obviously not get sufficient water due to included air A8 was watered much later and suffered also from air inclusion in the fresh water reservoir Fault Tree Analysis The QD swap was already mentioned as a potential root cause in the FTA RD1 and so included in the crew inspection activity to recover EMCS Unfortunately this failure was not detected at this opportunity Dok Nr No EMCS RP 0000 149 DOR c Failure Analysis EADS m d Report Me n Ausgabe lssue 1 Datum Date 1 8 2008 SSEri urm Seite Page
155. nts Applicable Documents lap Document Number AD01 CW RW ISR 16 02 07 Issue Title EMCS Cell Wall amp Resist Wall INTEGRATED SCIENCE REQUIREMENTS DOCUMENT AD02 EMEX RP MULT 02 11 12 03 EMCS Experiments Multigen Part 1 EADS Design Report EADS AD03 RP 30193 PR 02 06 10 2005 EMCS Experiments Multigen 1 Design Report CCNO4 AD04 N USOC REQ 007 06 07 2007 1 EMCS CW RW Integration Requirement Document ADO5 EMCS MA 4000 002 DCR 12 11 2004 EC User Handbook ADO6 EMCS MA 0000 001 DOR 18 05 2006 EMCS Payload User Manual ADO7 EMEX MA MULT 01 PRO 14 12 2007 PCC 1 User Manual AD08 EMCS PR 0000 07 DOR 21 06 2006 1 EMCS EM Systems Start Up and Stop Procedure ADO9 N USOC PRO 002 14 05 2007 PCC Assembly and Seed Set Up Procedure CW RW Seed Set Up ch Ltd Miss We Procedure bermriiss Ure Eesen asg Beamten Geste E rit 2 List of Abbreviations EC EM EMCS PI PA QA ESA NASA IOT N USOC Experiment Container EMCS Engineering Mode European Modular Cultivation System Principal Investigator Product Assurance Quality Assurance European Space Agency National Aeronautics and Space Administration Industry Operator Team Norwegian User Support and Operations Centre N USOC PRO 004 Issue Issue 1 Date 16 01 2008 Page 5 of 13 N USOC PRO 004 CW RW Seed Set Up Issue Issue 1 Procedure D
156. o IV ul I UOIJELIBA 9Jnpo20Jd 1591 Z 99 Jo p eBed 100Z S0 z oe SUBJQUSN vAd jo uogezije3s pue Bu pinoiN CL anssi r xipueddy vFO0dV Z00 OHd DOS rN Inravatibn and Creativity 3 Procedure Sign off N USOC PRO 002 AP05 Appendix x 4 Issue 1 3 iti i Date 23 05 2007 Moulalcay and StcaLiZachon Page 48 of 56 Pv A m brane All steps and activities described in the Procedure have been successfully performed Failures and non conformities raised during procedure execution were listed in table 2 Procedure sign off Engineer e ka n 4 Quality Assurance N A Date Date N USOC PRO 002 AP05 Appendix 5 Issue 1 3 Borosilicate Cutting Date 23 05 2007 Page 49 of 56 Appendix 5 Borosilicate cutting 3 N USOC PRO 002 AP05 tneovation and Creativity Appendix 5 Issue 1 3 Borosilicate Cutting Date 23 05 2007 per Page 50 of 56 Appendix 5 Borosilicate Cutting Prepared by H kon Svare Reference Issue 1 3 Date of Issue 23 05 2007 Status Document type Procedure Distribution Sei NTNU N USOC PRO 002 AP05 latcvation and Creativity Appendix 5 Issue 1 3 Meutding of PVA Membrane Date 23 05 2007 3 Page 51 of 56 Baresikcate Cuttin 1 Procedure for Borosilicate Cutting lt q Inner Hole Punch L S lt q Guide Sleeve F Outer Hole Punch a Borosilicate Fibre Sheet LA 1 Pick up the punching tool by the Outer Hole Punch and
157. ocedure Date 27 02 2008 Page 6 of 58 3 Introduction The CW RW hardware consists of the CW RW Plant Cultivation Chambers PCCs integrated in the CW RW Experiment Containers ECs The CW RW ECs have been shipped from Astrium FN Germany to Prototech Bergen Norway where the PCCs were implemented The CW RW ECs with implemented PCCs were then shipped from Prototech to the Norwegian User Support and Operations Centre N USOC TRD Norway After arrival at N USOC the CW RW ECs with PCCs were utilized in CW RW Biocompatibility test and CW RW Hydration germination tests At the end of test period the ECs and PCC were cleaned by Prototech and finally surface sterilized by N USOC set up engineer In the period from 14 24 of January 2008 one Pl team representative and one N USOC engineer performed set up the PCCs with growth support and Arabidopsis seeds and closed the PCCs according to AD02 and ADO3 as documented in ADO4 IOT performed a final verification and leak check of the ECs at N USOC as documented in ADOS Table 1 shows an overview of the needed documentation of the work performed at N USOC as part of the CW RW Flight Hardware preparation Figure 1 CW RW Plant Culture Chamber implemented inside CW RW Experiment Container EC N USOC PRO 005 N US0C rm CW RW Flight Sample Hardware Packing Transport issue 2 BRarwiiss ser kapper and Operttions Fraien and Check Out Procedure Date 27 02 2008 Page 7 of 58
158. ositions on each rotor A1 A3 B1 B3 lefty and WT are observed every 6 minutes during shoot circumnutations period 5 10 days EC position and timeframe for observation can be defined near real time 3 day lookahead in flight EC conditions should allow clear view of the emerging shoots The still images are downloaded via telemetry 1 Observation was prohibited upon several occasions 2 There was no stem to observe Payload anomalies PAR EMCS SW 001 PAR EMCS SW 002 PAR EMCS SW 003 PAR EMCS SW 008 PAR EMCS SW 009 Due to the fact that in the end only one plant was growing in all of the ECs resources were redirected to observation of this particular EC Payload Anomalies and ESA PI agreement Harvesting APOS 1 CWRW EIR Exec 17 2 CWRW EIR Exec 18 3 JCWRW EIR Exec 19 1 The samples from Rotor A rotating 1xg shall be retrieved first by the crew after the experiment stop 2 EMCS CW RW samples from Rotor A must be removed from the EMCS ECs fixated and placed into MELFI for preservation within 1 5 hours after the crew removes the ECs from the EMCS 3 EMCS CW RW samples from Rotor B must be removed from the EMCS ECs fixated and placed into MELFI immediately after samples from EMCS Rotor A have been inserted into MELFI and within 1 5 hours after the crew removes the ECs from the EMCS 1 8 2 There were no plants on the A rotor for harvest 3 The CW RW Experiment was concluded by harv
159. peak L k enfeA anjeA SyJELISY j rjoy SIOUBL13 O BUIMION uonduoseg dajs j531 on dajs 3153 enpaooJd Sy 0 SUOYHBUEA pue SabuEUJ z SIGE S e qe ui peyueuunoop eq eys enpaoo4ec SY 0 suoneueA pue seDueuo jy Ul J UONEMEA gINPaJOJJ 159 Z 95 jo Og abed i oz gtr exq JUSLUYSHUJ uonignw 911087 L xipueddy CL enss VOdV c00 ONd OOSfrN 4 N USOC PRO 002 AP02 lagoyation and Creztteit Appendix 2 Issue 1 3 rfiltering of Date 23 05 2007 Zeolite Page 31 of 56 A Enn chment 3 Procedure Sign off All steps and activities described in the Procedure have been successfully performed Failures and non conformities raised during procedure execution were listed in table 2 Procedure sign off Engineer Date N A lt EE tes be en H een en mn mm Quality Assurance Date N A N USOC PRO 002 AP02 lageyation and Creativity Appendix 2 Issue 1 3 Procedure for filtering of Date 23 05 2007 Zeolite Page 32 of 56 Appendix 2 Procedure for filtering of Zeolite vr QUI MEG a wo N u ing o EMCS EXPERIMENTS Procedure for Filtering of Zeolite EMEX PR MULT 04 PRO Procedure for Filter Doc no EMEX PR MULT 04 PRO prototech EMCS Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 Page 2 of 19 PROJECT EMCS EX
160. peey youne snqunjog LLO MH UVd STLLND 9cLLINS rzi LWO Gd SYO SOW3 9ZLLWS JejSuB y SIH pue ajpAd Jemod SOWA p lu uu j duu LLO MH UVd SCLLWO 9ZLLND CLLINO sdo SOW3 SZ LUS uo Me dems 23 eui Jaye papesu jy eseqejeq yu dn allg 10 1senbay p lu uu lduu LLO MH UVd S LLWS 9 LLWS p lu uu jduu VeLLWO p lu uu duu ETLLND Ad dey Id za d 01 Zv LV suonisod Wou s93 dems dems 93 ann maid 104 jsenbay yoiym Bnq ay urejuoo jou saop uou einpeuos SL HOIT MEN LLO MH UVd LZLLWS pajuswajdwij FLO MH UVd tzi LWO te LLWO day ld Jejem p u sjuejd eui JEU peyeo pu uorum Butpjoj aq oj waas Sange rg PUB g o sas nd Jejem S ppe o senbay pejuawajdu LLLWO cLELWO dos Aem jey ay 0 no pasjind uaym Ayianou mass pajuawajduy 8 Jojo pue y 4001 jo sefew jenuew aye 0 J ayi pajsanbayj edejday say OZH ay Guunp sudeiBojoud 10j jsenbay Sey GHW eu uonnjoss1 peg anb seu snquinjo ui Wegqe au souls LLO MH YVd ELLLWO 60LLND des d ad nebr 1 amp 4 joe4 JO Wed eoe day say OZH AWARD M12 10 Jsanbey o LZLLWO dey la Jejem peeu sjuejd ay yey payeoipul yorum Huipjoy aq oj waas seAee rg pue eg o sas nd Jejem S ppe o isenbay p lu uu duu LLLLND OLLLND day Id SJUe d ya jo sniejs eu Yoayp 0 JepJo U O pIA LY pejsenbau O8pIA SON eUeju yo senbay pejusuajduy Wea la ay pue juewou y Je Buipuodse uou sem AA SUL OLLLIND 60LLINO V 1010 UO SeXOq SS 1g eu Bulyoyms AIBA Buunp meup zuana jo UONEASSAD pajuawajdu
161. perly installed The pressure sensor hole is also covered by the borosilicate dL Dok Nr No ST Trouble Shooting for NE ui EADS rm SPR 283 Ausgabe lssue 1 Datum Date Seite Page 18 von of EMCS RP 0000 151 DOR 18 8 2008 24 Fig 13 Fig 14 10 weighing of growth pot incl PP felts PVA sponge All parts were dried for 24 hours at 50 C with weighing before and after drying The following weight losses were measured Zeolith 2 3 gram sponge 0 2 gram felt 0 0 gram JL Dok Nr No EMCS RP 0000 151 DOR Sa Trouble Shooting for EADS j Ausgabe lssue 1 Datum Date 18 8 2008 SSErium SPR 283 Seite Page 19 von of 24 6 WATER RESERVOIRS 6 1 FMOO1 Inspection of external surfaces and QDs showed no damages on housing and QDs After opening of Water Reservoir an air bubble was seen in the fresh water bag This confirmed that air water was pumped into the fresh bag during dehumidifier draining of the EC positions A1 A2 as to be expected for the case of swapped QDs at the RBLSS Mod No corrosion was found inside or outside the waste housing This confirmed that the new material and surface treatment shows better corrosion protection than the original design The waste sponge was removed It was humid but did not contain squeezable water which is compliant with the analysis of 0 12 g expected contents with the swapped QDs of RBLSS Module FM008 and FM007 see Table 6 1 The
162. pip uoneupAH Jejem UMA SSTEY 0 JIOMSSEY OZH Wo s qni UU 0 Jdwany suonisod 93 Jeyjo y u EUILUOU sseJBoud jou pip UONEIP H suonisod 93 Jeyjo ay ui Ajjeulwou ssai6oud jou pip uoneip H suonisod 93 Jayjo ay u BUILUOU sseJboJd jou pip uoneJpAH aduvyo 10 UOSEIY IS way UONDY s dwaye uoneup u 6xg 0 Jaye v Jojo Jo uonejo J dois sjduaye uoneLpAy 6xg Jaye v 40103 JO uonejo doe sas nd 001 5xg Q ur urebe ainpayos uoneipKu Jeuuou uuojeg sas nd 001 5xg Q u uieBe ejnpeuos uonespAy jeuuou woad sas nd 001 6xg 9 u Uebe ejnpeuos uonespAy jeuuou woad sas nd 001 5xg Q ut urebe ejnpeuos uoneup u jewsou WIOHST 66 0 Ja UNOD jas u u ses nd 97 Joj uni 19 uleBe ainpayos uoneJp u uiejses uonnedai yoea usamjaq sBunjes an en Bui amp Bueuo jnouym sawn c Jeada em sejnuju c Udy UIL JO uni duind Jejem au 191 yuljdn aji 40 jsenbay ses nd 62 6xg 0 ut ejnpeuos uoneup u ewou uoned sasind 6xg 0 u ejnpeuos uoneup u j euuou ulojJed ses nd 6xg ui ejnpeuos uoneupAu jeuuou WHOL ses nd ce 5xg 0 u ainpayos UONBIP Y jeuuou WIONSd ses nd ce 5xg 0 ui ainpayos uoneipAy jewsou woed zduung Joj uiui dung JOJ uiw g 10103 uo xog uo Bx ui ajsem o usaJj Wouj Jajem dwnq ses nd 001 5xg 0 ut ejnpeuos UONBIP Y jeuuou WIOLaJ sasind 00 Bxo u ainpayos uoneJpAu jeuuou WONad ses nd 00 5x0 u ajnpayos uoneJpAu jeuuou ulloH d uonisod ISULI JO UOHdLIESAGA 24 Buneap Ssoul
163. pport and Operations Centre Table 3 3 Considerations discussed during the CW RW EST and documented in the EST Report Samfunnsforskning AS EMCS CW RW Requirements Integration Report Doc Nr N USOC REQ 025 Issue 1 Date 28 11 08 Page 13 of 30 Objectives Comment The EMCS CW RW tests and the schedules have been evaluated to meet the scientific objectives in EMCS to support stem development of the 4 different Arabidopsis thaliana wild type hmg GUS Wild efty under the environmental condition requested by PI N USOC TR 25 RDO1 N USOC TR 28 RD02 N USOC TR 29 RD03 The testing which has been performed in the EM has covered the whole duration of the experiment in sequences The Water Strategy and Water Budget estimation N USOC NOT HS 002 RD04 is a result of these tests Schedules used during these tests will be the basis for The Flight Schedules for the CW RW experiment on orbit Some alterations need to be made for the schedules to be made applicable for the whole run positions watering etc These Flight schedules are not complete and are an open issue which will be closed before flight NOTE As part of the EST or Pre EST testing there has not been performed a start to end sequence test of the experiment run in the EMCS GM using the EMCS schedules that shall be used in Flight with the parameter setting defined in the Water Strategy and Water Budget Arabidopsis thaliana shall grow automa
164. quests The discussed solutions had not been tested on ground to a satisfactory degree and unforeseen issues arose as a consequence of this when the changed requirements were implemented The problems encountered during the CW RW Experiment on orbit have been documented in the NASA PAR and ESA SPR Systems Resolution of the open issues will be posted in the same systems Norwegian User Support and Operations Centre Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 E Integration Report Date 28 11 08 S Page 5 of 30 Samfunnsforskning AS Table of Contents SUMMANY NETTE 4 Table ot Gontents uuu oi ak a ne euere 5 Per Jo EE EE ME 6 Applicable and reference documents esses esee entente entree nnns nnns entree 7 We SCOP LEE 8 ME POE OSG C T RE 8 1 2 Document EN MT 8 2 see Rm 8 2 1 Scientific OD POC VE e Rn 8 2 2 Experiment Unique e Mee 9 NN Operational SN 9 3 The Experiment Requirement Integration Process as Periormed nn 11 3 1 CW RW Integration Testing in EMCS Ground Models 11 3 2 CW RW Experiment Sequence Test EST 12 3 3 Overall activities and products related to CW RW Pre flight Operations 14 3 3 1 Set Up and EUE Verification 14 3 3 2 Transport and Checkout Activities m 15 3 4 CW RW On Orbit Operations una nenn ne 17 3 5 Overall a
165. r the without the written and is to be used by the reci part by any other pa was supplied It shall not be disclosed in whole or in i S A 1 amp EM E i Doc no EMEX PR MULT 04 PRO IF CS ents p fo t O t ec h purum Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 Description Page 11 of 19 3 3 Start filtering the Zeolite by moving the FJ from side to side by using your hands See picture below Continue this to you observe that no more Zeolite is falling through the mesh in the FJ The QA engineer shall also observe and agree when the filtering is sufficient Move FJ from side to side Filtered Zeolite below 0 5mm diamter 4 Pour the filtered Zeolite above 0 5mm diameter into a clean container or glass See picture below The information contained herein Is proprietary to PROTOTECH AS and is to be used by the recipient solely for the purpose of which it was supplied It shall not be disclosed in whole or in part by any other party without the written permission of PROTOTECH AS Doc no EMEX PR MULT 04 PRO prototech IP EMCS Experiments Int Doc no PR_30193_PR_05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 Description Page 12 of 19 5 The Zeolite is now finished filtered and ready for use Filling of growth pot shall be done according to Growth Pot Filling Procedure f
166. rch 6 2008 The ECs were surface cleaned figure 3 3A and the ECs were placed in NASA approved Zip lock bags figure 3 3B Sharp edge and cleanliness inspections were performed No anomalies were detected E A Figure 3 3 Surface cleaning of ECs and placed in NASA approved Zip lock bags Late load of the samples L 10 was required in order to avoid long time storage in EUE prior to Experiment start The assembled CW RW ECs were handed over to Flight Crew Office on March 10 2008 where they were packed in soft stowage before launch on March 11 2008 18 days prior to experiment start Doc Nr N USOC REQ 025 Issue 1 Date 28 11 08 Page 17 of 30 EMCS CW RW Requirements Integration Samfunnsforskning AS Report N USOG ko Norwegian User Support and Operations Centre i 3 4 CW RW On orbit Operations The CW RW on orbit activities are listed in table 3 7 The related products are referenced Table 3 7 Activities related to on orbit CW RW Cultivation and stowage Activities Product Procedure CW RW KFT transfer to MELFI Crew Proc Initial insertion in MELFI 2 001 CELL WALL RESIST WALL KFT INITIAL MELFI INSERT MGUEEXPRSCWRWNOO1 EMCS Preparation Crew Proc Opening of Gas Valve 2 024 EMCS Valve Movements for Ground Commanding MGUEEXPRSEMCSN024 Install CW RW ECs in EMCS Crew Proc Replace Ref ECs with CW RW ECs 2 005 EC Replacement MGUEEXPRSEMCSNO05 Automatic hydration
167. ring of silicone 33 31 Performed step 33 before step 31 Surface sterilized the inside of the EC cover base plate grid and PCC too 35 After implementing the PP felt in the recession on the outside of the growth pot the pot was pushed down in the PCC until the o ring was aligned with the top of the PCC before applying grease to the o ring To avoid getting grease on PP felt and also to prevent fibers from the felt to attach on the greased o ring 40 After applying silicone to green sealing frame the EC cover was mounted on backwards and then opened for a visual inspection of imprint that the grease made to the cover to verify an even distribution along the sealing After this the cover was mounted the correct way 7 Non conformities No NCR was opened as a result of the changes and variations listed in Table 2 N USOC TR 027 CW RW Issue 1 PCC Assembly and Seed Set Up Report Date 13 02 2008 Page 9 of 105 8 Results The set up was performed according to procedure AD04 and ADO5 with changes and variations listed in Table 2 9 Conclusion No negative effect on the performance of the Cell Wall Resist Wall experiment could be identified from the changes and variations listed in Table 2 N USOC TR 027 CW RW Issue 1 PCC Assembly and Seed Set Up Report Date 13 02 2008 Page 10 of 105 Appendix 1 Filled in N USOC PRO 002 Multigen 1 PCC
168. rj207 ay uajsey E edoeuns E ve do 32d 94 0 umop y usnd uay pue suid IE OM eui eAoqe AINAIATALATALA na juo ay Wouy 1 Buipiis Aq 99d eui jo ao Burjoo eui JUNON 9 uopnduoseg dajg dms 96 JO ZZ abed ZE SIgeL 2002 S0 c aeq SmasOS INO OOOO ES OOS peuuojegd xJeuimM ojyoug roueg CL anssi sinpedould d 1 1eS pass pue Ajquiassy 39d L ueBn niy 200 OHd DOS FN aoueinssy Aen uone2o01 eg A0 peulojiag yoveasex lou Sad 0 V HE 9010 1 ON ug Buisse6 yo pue amp uuno 101 molje 0 JepJo Ul spr UIA uo uoollis Buldjdde veye sinoy z wnwiunu peuuoled eq jsnui siu LOQY yooqpuey Joen 23 0 Bulploooe 18402 23 y Junoyy esea1c winnoe USIH SUOHNS JJJM uosys uy u 5 awe Bupes 93 1602 pauuojiag y1ewsay ojoyd owaq WUOD p SIGE 96 JO z abed 4002 90 EZ ed CL anssj eunpeo2o0Jg AN I9S pass pue Ajgwassy Id L ueBn iniy c00 OHd DOS t N A0 pauwojsq eoueinssy Eent uone3o1 sed SI gt 8010 nz 3239 Pral DaoTwo WON VON GOO ans TY rwv o y Vo yy al nfu Oe 222 SY quq 29922077 15 0400 nt 226 Op kp ooo p Jo Shad uo umo yassaid nan area solar um Di 24L pay out OU sa way buu o da un amd oye m mam xv apb a vwmunds zus pwoz dunzio JODJL UU HJ Pas yen usd eue Joa CaS Eau bed yma yum bo oi ribon veasnfyu gt Ao LOC mort ua Ans 2 atm ca nod s DDR ATOT can Of 3230 ON
169. rocedure RD8 EMCS PR 0000 010 DOR issue 1 RBLSS QD Exchange Procedure RD9 EMCS TP 0000 135 DOR issue 1 RBLSS System Proof Test Procedure 3 RETURNED ITEMS After use in EMCS the following items were returned from ISS to ASTRIUM and used for inspections and test as part of the trouble shooting for SPR 283 ITEM CWRW history pu initially on Pos A1 germination but no successful watering finally on Pos B1 FM092 EMCS EC 401 000 watering retrial not successful initially on Pos B2 no successful watering DE a finally on Pos A2 watering retrial not FM099 EMCS EC 401 000 successful successfully used on earlier mission second mission after refurbishment mounted on rotor A in exchange with FM008 no FMO001 EMCS WATER RESERVOIR 223 200 successful watering me pwen peu first mission initially mounted on rotor B no successful watering during CWRW exchanged FM009 EMCS WATER RESERVOIR 223 200 for FM002 sume mcsamesuonue zu eee FM008 EMCS RBLSS MODULE 223 100 pos B1 B2 no successful watering 4 COMBINED TESTS WITH RBLSS EC WATER RESERVOIR 4 1 RBLSS GSE Start up Sequence Start up of RBLSS GSE OHB owned equipment 1 Connect RBLSS connectors P12 and P14 with GSE harness 2 Power on both GSE computers no matter sequence and wait for start up takes approx 2 minutes 3 Switch on Breadboard E Box 4 Double click on icon Breadboard MCS GSE Switch off OHB GSE 1 Exit Breadboard MCS GSE program EADS
170. rom Multigen 1 PI Zeolite ready for use above 0 5mm diameter The information contained herein is proprietary to PROTOTECH AS and is to be used by the recipient solely for the purpose of which it was supplied It shall not be disclosed in whole or in part by any other party without the written permission of PROTOTECH AS SV HOALOLOYd JO uolssjulled usnm eu MARIM Aued sawo Aue Aq Hed uj JO ajoum uj pasopsip aq JOU Heys 31 pajddns sem 3j u iu A Jo asodind ayy Joy Ajajos quajdjoas ayi Aq pesn aq 0 si pue SY HIZLOLOYd 03 Alejandould s uialay paujeyyuon UOPEULIOJUI aur pausiuy Bue S days os 4 Lal del Z S Jaidpu o3 23e Bullayy 31835 T m La d 1 E 3 Lj ki Li ki ka y Jaqdeyo ut 3sr queuudinb3 no pue T g Jejdeyo o3 Op Juswd nba y y 9 gt Jejdeyo ui jeuuossad 3no j T suonmneJedaug uondinseaq dois ul Suena JUNAADOUd dils Ad dILS 9 Er jo ET aed NANAaFBIOUd dALS AY dai ls EGTOE olg 200Z 50 E0 eme g nssI 21211032 JO Buii 3li 40j o1npo204gd SO Hd EGTOE Wd ou 20C Ju syu uuri dixg SJNI EI u22303oJd Oud PO JANW d XIWI ou aoq SV HJHLOLOUN Jo LOJSS LS0 Una ayi NOY UM Aued sawo Aue Aq ued uj Jo ajoum uj pasopsip aq Jou jjeys 3 pajddns sem 3 YDIYM jo asocund ayi 40J Afa 0s 3uajdi2a ayi Aq pasn aq 01 si pue sy HI3LO1OUd 03 Atejaudoud s jasay paujejuos uaneuuojul au 3eeus 4O UBIS 34npa204d UIS pue 3no ju TI SLO s
171. s 4CLLWO Els LLO MH UVd OELLIND ZZLLIND LZ LUND gYN Ad pauawajduy UONEUNSOG OS speau 1 USYM useij WO Jajem sief Jay p wnyap ay ji JU A 0 158 1ulod 18s 9 0 7 uuojieg OELLIND ZZLLWO LLLND SUN Ad uonebsanu eunjie Al S EP S JO a sem eq Pinoys pajuewsjdwij UONBUNSSQ OS spsau j USYM usaJj Wouj Jajem sab Jeyipiuunuep eu H Ajuen 0 159 juiod jas 9404 load ZZLLND ZZLLND gywad uoneBnse u ane A S ep S 104 a sem eq pjnous uoneunsag OS speau j USYM usaJj WO Jajem sief Jeyipiunyap eu J AJue o Isa jurod 18s 9 07 uuojieg SZLLND Z2LLWO FCL sdo SONI yu dn 9j JO 15enbes IO GIU PES 2 A UVd paier Sms aep ong OST 10 EUISIIG SUEI3 10 LOSEIY aduviyo Jo uondrios q LLO MH YVd OE LLNS LZLLWO pajuawsjdwj wonebnsaAyj anes EE Jo Ned LLO MH UVd LLO MH UVd OE LLIND pajuawajdu e pue s lnp uos S HON sainpayos LN3A3 Sulpnjou seji mou 6 uonsod SKI Y JO S abed si7 Way UONDY Buneap sseuipees youne7 snquunjog ELO MH SOWF3 ELO MH SOW3 L0 MH SOW3 LLO MH SOW3 ELO MH SOWF3 LLO MH SON3 L0 MH SOW3 LLO MH SOW3 L0 MH SOWA LLO MH SOW3 ELO MH SOWJF3 LLO MH SOWJ3 LLO MH SOW3 LLO AH SOW3 LLO MH SONW3 UVd PEPY yL JO 9 oBed op lu uu duu cpejueuiejduuj HP LIND pajuewajdw cv ILIO pauswajduy Sv ILLINS GH LIND LIND 2 LIND e WLLNS ad dey Id OP LIND dey ld OF LLND day Id BE LLIND dey Id 6 LLINS d
172. s at handover to SSL N USOC representatives will clean the ECs at KSC The CW RW flight ECs S N FM092 FM094 FM100 will be inspected at KSC after transport from N USOC to ensure Everything looks like it did before the transport All surfaces are clean Protective sheet will be removed from EC Covers N USOC PRO 005 N USOC mW CW RW Flight Sample Hardware Packing Transport issue 2 a W and Check Out Procedure Date 27 02 2008 Page 12 of 58 6 1 Procedure Equipment 6 1 1 Article List Table 5 Article List Number CW RW ECs with PCCs ECs PD and samples cleaned and PCCs PD placed in zip lock bags PCC assembly growth medium and samples PI and N USOC representatives and N USOC EC Check IOT transport boxes with foam for CW RW ECs 25800 6 1 2 Equipment List Table 6 Equipment List Article Name Supplied Serial Available b Number A Packing Procedure at N N USOC USOC B Transport Procedure N PRO 005 USOC to KSC Transport boxes with foam N USOC C Checkout Procedure at N USOC En Sm we WA s av C 30 une Ld EL 4 Le CO Ae so mm Mud amp xog uodsuei ojui s93 MY MO Z IIeisul Z xog 1iodsuei so ei xog Hodsued ui 960 9604 N S 23 Joj jeeys 607 Svele 860 960IN4 93 MU MO 104 jaays Dot ut H z xog 11odsuei ui 12660 s nyessdwus su e GJE P zi Z xog H LL ul Suny LL JESU cit xog uodsuei out SOF MY M Iesu L t xog Hod
173. s to be used by the recipient solely for the purpose of which it was supplied It shall not be disclosed in whole or in part by any other party without the written permission of PROTOTECH AS Doc no EMEX PR MULT 04 PRO prototec h x EMCS Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 APPENDIX C Page 19 of 19 APPENDIX C PROCEDURE SIGN OFF SHEET Item Part No Serial No has been filtered in accordance with the foregoing procedure ENGINEER N A Sign Date PRODUCT ASSURANCE V A Sign Date PROJECT MANAGEMENT Sign Date CUSTOMER REPRESENTATIVE Sign Date where applicable The information contained herein is proprietary to PROTOTECH AS and is to be used by the recipient solely for the purpose of which it was supplied It shall not be disclosed in whole or in part by any other party without the written permission of PROTOTECH AS b N USOC PRO 002 AP03 Insovation and Creativity Appendix 3 Issue 1 3 Fixation of Sponge on PCC Date 23 05 2007 Growth Pot Page 33 of 56 Appendix 3 Fixation of Sponge on PCC Growth Pot Appendix 3 Fixation of Sponge on PCC Growth Pot N USOC PRO 002 AP03 Issue 1 3 Date 23 05 2007 Page 34 of 56 Appendix 3 Fixation of Sponge on PCC Growth Pot Prepared by Reference Issue Date of Issue Status Document type Distribution Suzanne verlie 1 3 23 05 2007 Procedure
174. strategy when November November N operating the ECs with 5 14 USOC mature plants and utilizing the EMCS water TR 24 recycling mode channel excess water from dehumidifier to humidifier and EC 3 Biocompatibility ERM Test and verify the functionality performance of December December N Germination Test the CW RW PCC ECs when cultivating 11 20 USOC Arabidopsis thaliana from seed to early TR 25 development of shoot Test and verify biocompatibility of the CW RW PCC ECs when cultivating Arabidopsis thaliana from seed to early development of shoot 4 Rewatering EM Verify the control strategy for automatic water January February N Verification Test supply to the PCC 31 18 USOC Validate the different regulating schedules with TR 29 the selected set points Confirm the estimated water budget with the selected automatic watering control strategy 3 2 CW RW Experiment Sequence Test EST The tests were finally reviewed during the Experiment Sequence Test EST from February 19 to February 21 which was a compressed table top of all procedures and products related to the CW RW Experiment The main conclusion from the EST was that the EST was a success and that all parties recommended continuing with CW RW Flight preparations with some necessary updates to procedures and products Considerations mentioned in the EST Report can be seen in table 3 3 N USOC m3 Norwegian User Su
175. success in watering B1 B3 B4 Since B3 B4 watering followed immediately the water reservoir exchange only some air was collected in the fresh water reservoir and the positions could receive some water B1 did not show a proper triggering but a kind of partial triggering which is explained by a mixture of air with some water which led to this behavior A4 was also watered quite soon after water reservoir exchange but did obviously not get sufficient water due to included air A8 was watered much later and suffered also from air inclusion in the fresh water reservoir Doc Nr N USOC REQ 025 EMCS CW RW Requirements Integration Issue 1 Report Date 28 11 08 N USOG m3 j Norwegian User Support and Operations Centre GR Page 29 of 30 Samfunnsforskning AS 5 Conclusion One of the difficulties of the Cell Wall Resist Wall experiment Integration was the late delivery of CW RW test hardware CW RW is one of the longest experiments ever conducted in the EMCS on the ISS and the test plan was affected by the length of the experiment The EST was performed as a Table Top so the full experiment run with 8 ECs was thus not performed due to time constraints No problems were encountered during flight preparations at N USOC hardware check out transport to KSC or CW RW experiment handover to NASA During CW RW experiment execution on orbit 15 of the 31 CW RW Experiment Requirements were changed or broken and the main experiment ob
176. successfully performed Failures and non conformities Procedure sign off Engineer Date o d Que bandet 04 feb 208 Quality Assurance Date b Lie taste e OG Febina i 1008 males or rm am mm ma am e mer ere m em im re or N USOC TR 027 CW RW Issue 1 PCC Assembly and Seed Set Up Report Date 13 02 2008 Page 86 of 105 Appendix 2 Filled in NLUSOC PRO 004 CW RW Seed Set Up Procedure Issue 1 N USOC PRO 004 CW RW Seed Set Up Issue Issue 1 W Procedure Date 16 01 2008 Hp enpster Begpert and Operations ste OO 3 Page 1 of 13 CW RW Seed Set up Procedure Prepared by Suzanne verlie Reference Issue Issue 1 Date of Issue 16 01 2008 Status Document type Procedure Distribution As described in Approval list page 2 N USOC The Plant BioCentre Dragvoll Alle 38 N 7491 Trondheim N USOC PRO 004 CW RW Seed Set Up Issue Issue 1 Procedure Date 16 01 2008 Page 2 of 13 Approval Title CW RW Test Procedure Issue Draft l CWIRW Seed Set Up Author Signature Affiliation Date Suzanne verlie ug Curly N USOC ib of Checked by Signature Affiliation Date c e Al 08 Ann lren Kittang Ann ren klang N USOC l 8 Pes Knut Fossum Mau EQ Raum N USOC Manager V vg Motoshi Kamada deals ludas JAXA CWIRW Ka og Team Coordinator Ulrich Kuebler ESA EMCS CW RW PIM w 23 a Por Wilfried Biemann E R Astrium IOT PA e Thomas Niedermaier Astrium Approved by Sig
177. suel aso Lit xog uodsuei Ul G60WH 760W4 Z60IN4 O3 Joj 2345 807 eoeld S60IN4 v60IA4 Z604 93 MH MO 104 jeays Bo ut IIIJ p a L t xog podsueu ui 12660 einjejeduie jersu 22 07 n A sBeq oo diz u 560 W4 760WNH Z60IN4 N S S23 Lit Xog podsuesj ojui s93 MY MO Iesu DOSN N JE e1npe204gd Bunjoed UI enpeooJg 7 Age Z 9 qe ui pejsij ere sdajs 159 Jene ui4eJnpeoo4d Z 8540 abe 8002 20 Zc aie eunpeooJg NO y9eUD pue UE DOS AKK 9n58l yodsue1 Bunjoeg asempsepy ajdwies W I MWA JOSA N S00 OHd OOSf N eh Pa eu 2 PNS SBeq 420 diz pepi oud YSYN ul SOF 24 evejd SJ AOD 23 UO Dol 8Aj2ejoJd SAOWAY L AL anssi seu juaj pue jouedoJdosi asn YSWN Aq pajsanba pepeeu si Buiueejo mau J sseuiuee p Jo seoeuns 23 E pey SOOd UM S93 MH MO JO uonoedsur ENSIA OSM JE e1npe204g 3n032eu5 9 abue 9 D ZZ BU JO no seinjejeduie piony euin Aue je peuedo eq jou jsnui 23 au seuinjsoo Aunoes uonoedsut Jo peuedo aq Aew sexoq podsue y FI aBuel 9 8 D ZZ BY Jo JNO seunjejeduie piony 2SM 0 JED q sexoq podsueun e eu uodsuei suejdue uo sejdwes u s93 Uu sexoq podsueu e y Aueo pueH eBuei 9 i O ZZ BU JO JNO senjejadwa pony A N Sastre psugopsa uondii9s q Ieguonippy PE KA Wan SS c xog uodsuej ui 32660 einje1eduue e3su LII d ES 8940 p abed PSS A AN tm
178. t until it is returned to Japan The Seeds are installed in the Experiment Containers at the Norwegian User Support and Operation Center N USOC located the Plant Biocentre NTNU in Trondheim Norway Transport The samples will be handcarried to Kennedy Space Centre in foam coated suitcases see figure 3 By car from Trondheim to Trondheim Airport V rnes by Airplane from Trondheim Airport V rnes to Orlando Sanford Airport by car from Orlando Airport to NASA Kennedy Space Centre FL At arrival at NASA Kennedy Space Centre KSC FL the enclosed Cultivation Chambers will be stored under the custody of NASA KSC under agreed ground handling procedures The same will be the case for Kennedy Fixation Tubes after return to Earth US KSC before samples are transported to Japan N USOC PRO 005 N USDE Mile op CW RW Flight Sample Hardware Packing Transport issue 2 mA and Check Out Procedure Date 27 02 2008 Aeren Buer Lansert and Operations Center UDE Page 57 of 58 Figure 1 The Arabldopsis seeds installed In the enclosed Experiment Container E gt E e Figure 2 Kennedy Fixalion Tubes showing how the plant samples will be contained in fixatlon liquids The KFTs have sets of 3 o rings to contaln the samples N USOC PRO 005 mmm ne CW RW Flight Sample Hardware Packing Transport issue 2 Nacwtilss Diss Lansert and Qeerations Centr and Check Out Procedure Date 27 02 2008 Page 58 of 58 Dear Ms Rostad Th
179. tching tab ok for each pulse switch water pump B1PW2 ON for 15 seconds observe water bubbles entering EC and note estimated volume in Pulse Record 65 GSE Pump Switching tab ok for each pulse switch water pump B1PW2 OFF B1PA2 remains ON 66 GSE Valve Switching tab ok for each pulse switch valves B1V4 and B1V6 OFF d Dok Nr No EMCS RP 0000 151 DOR EADS Trouble Shooting for SPR 283 Ausgabe lssue 1 Datum Date 18 8 2008 as E rium Seite Page 11 von of 24 67 Wait 1 minute for the first 25 pulses ok for each pulse wait 5 minutes for the remaining pulses ECDK 68 Set Digital Input 3 0 ok 69 Set Digital Input 2 0 ok 70 Set Digital Input 4 1 ok 71 Set Digital Input 5 1 ok 72 Switch EC Power OFF ok 3 switch air pump OFF ok 74 Dismount and weigh EC PCC 1741 6 g gt delta m 11 99 75 Weigh Water Reservoir as reference 1232 8 g gt delta m 13 69 Pulse Record Pulse pressure estimated water Pulse pressure estimated water analog 2 mV volume in EC analog 2 volume in EC 12 08 200 delta t 1min 13 08 2008 p RH PCC 8 1 770 0 2 41 812 2720 6 7 2 770 0 3 RH ctrl 50 5 minutes on only RBLSS to generate air bubble 3 776 0 3 42 817 2343 6 9 4 781 0 3 43 813 2290 7 1 5 780 0 5 44 815 2239 7 3 6 777 0 7 45 830 2207 7 5 7 770 0 9
180. the IOT HFIT and IPLAT were not part of N USOC responsibility 3 3 2 Transport and Checkout Activities The main activities related to transport and checkout are listed in table 3 6 The related products and performance documents are referenced and can be viewed in Appendix 3 The temperatures logged after set up and until after the stowage at KSC pending launch can be viewed in Appendix 4 Table 3 6 CW RW Transport to KSC Check out and Stowage pending launch Activity Product Procedure Transport to KSC Transport boxes Shipping papers N USOC PRO 005 Seed certificate N USOC PRO 005 KSC Preparations and Checkout 8 ECs with integrated EUE N USOC PRO 005 KSC Stowage pending launch 8 ECs with integrated EUE in transport bags The 8 assembled CW RW ECs were surface cleaned packed in Ziplock bags and placed in approved transport boxes BWH AZKE 95600 with protective foam Plastazote of more than 2 5 cm on all sides of the ECs Figure 3 2 The integrated ECs were transported to KSC by Airplane The ECs were not exposed to X Rays at any time during the transport NU Doc Nr N USOC REQ 025 EMCS CW RW Requirements Integration Issue 1 Report Date 28 11 08 M Page 16 of 30 Samfunnsforskning AS Norwegian User Support and Operations Centre Figure 3 2 The CW RW ECs were packed in Zip lock bags and transported in foam coated transport boxes per agreement with PD The check out was performed at KSC Ma
181. the SPLC because the SPLC did not receive TM from all subsystems connected to the NSP bus incubator for 30 sec All other subsystems were powered off simultaneously with the TCS as expected in such a case The subsystems connected to the other NSP busses and to the RS422 interface sent nominal telemetry and the EMCS power draw was nominal until the power down i e the TCS was still running nominally while no TM was received Impact on experiments temporary loss of environmental controls Remark It is taken into consideration that a common cause led to the incidents described in EMCS SW 0002 EMCS SW 0008 EMCS SW 0009 Obs target and freq EIR Exec 09 A restart of the EMCS subsystems was only successful after a power cycle of the SPLC 1 Resume VPU capability period with loss of environmental control 2 Loosing images during VPU lockup no loss of environmental control Gravity level EIR Exec 13 Illumination EIR Exec 15 Power interruptions EIR Exec 31 Obs target and freq EIR Exec 09 EMCS HW 0006 EMCS Humidity Control Issue The efficiency of the dehumidifiers of RBLSS Modules FM005 and FM006 was reduced Impact on experiments a limited water recycling capability b temporary clogging of air tube due to a water droplet Relative Humidity EIR Exec 3 In all cases the air flow could be re established after the droplet had evaporated Samfunnsforskning AS
182. thus fitting into fresh QD of the water Reservoir As it was confirmed by the supplier OHB the same discrepancy is most likely present on FM007 which was also used during CWRW and which is still mounted in the EMCS FM on rotor A Development of the failure Following the history of CWRW the following happened e Atthe beginning of CWRW the humidity RH control of all ECs were started with 50 setting Due to the QD swap no fresh water could be supplied to the humidifiers which was not immediately recognized because they contain sufficient water to supply 5096 humidity for quite a long time However the wrong connection led to an accumulation of an air water mixture 90 air 10 water volume in the fresh water reservoir instead of waste because the dehumidifier is drained every 3 hours e In parallel to RH control the initial watering of EC PCC was commanded starting with positions A1 A2 B1 B2 These positions now tried to get water from fresh reservoir but in fact were connected to waste reservoir which nominally did not contain free water at the beginning of an experiment So these positions only received the small amount of 1 5 ml water which was contained in the tubes e Initial watering of positions A3 A4 B3 B4 was started several days later Since these positions were connected correctly they tried to get water from fresh reservoir but received only an air water mixture which was partly sufficient for triggering the p se
183. tically from seed to approximately 10 cm in the EMCS Ground Models supported by the PCC Verified in Science Test N USOC TR 28 RD02 Seeds germinate in the ECs and confirm 1 2 ana Verified in Biocompatibility Test N USOC TR 25 RD01 biocompatibility 1 3 Water is supplied to the Growth Pot after the initial Verified in N USOC NOT HS 002 RDO4 watering by delta pressure early enough to support further growth of seedlings 1 4 Water amount is sufficient to keep plants vigorous The critical phases of the experiment as identified in Muligen 1 have without sign of exsiccation been tested in the EM and based on this the water usage has been estimated and a water budget has been made NOTE The EMCS FM and EM are similar but not identical and may result in a higher water usage In case of water shortage due to a potential difference in recycling compatibility between the FM and EM a strategy has been found to ensure further growth of plants N USOC NOT HS 002 2 The transport procedures the crew procedures OCA message will be finalized after growth development is and OCA message should have been evaluated established in real time There are a few open issues regarding the to meet the Pls requirement and ensure optimal Transport Procedures and the return of the hardware which will be samples returned to the Pls lab closed shortly These issues are regarded as normal open work 3 The procedures will confirm that the EMCS
184. til water comes out permanently 2 Weigh Water Reservoir 1280 7 gr 3 Weigh EC 1139 3 gr 4 Mount EC to Rotor I F ok 5 Start p sensor recording ok 6 At GSE start water service to EC 1 pulse 1minute pumping ok 1 pulse 83 sec wait 1 minute 7 At syringe read out total volume and collected water volume total 1 1 ml water 0 05 8 Repeat step 6 2 pulse ok 9 At syringe read out total volume and collected water volume total 2 5 water 1 0 adi Dok Nr No EMCS RP 0000 151 DOR EADS Trouble Shooting for Date 18 8 2008 SPR 283 Ausgabe lssue 1 Datum E r ILITTI Seite Page 8 von of 24 10 Repeat step 6 3 pulse ok 11 At syringe read out total volume and collected water volume total 3 9 water 2 2 12 Repeat step 6 4 pulse ok 13 At syringe read out total volume and collected water volume total 5 2 water 3 5 14 Repeat step 6 5 pulse ok 15 At syringe read out total volume and collected water volume total 6 6 water 5 0 16 Dismount and weigh EC 1145 4 gram 6 1 gram added gt 6 6 5 0 1 1 gram in filter 17 Mount EC again ok 18 Turn Rotor l F with EC 180 deg top down ok 19 Repeat step 6 6 pulse ok 20 At syringe read out total volume and collected water volume total 8 0 water 7 0 21 Repeat step 6 7 pulse ok 22 At syringe read out total volume and collected water volume total 9 4 water 8 3
185. trial not successful initially on Pos B2 no successful watering finally on Pos A2 watering retrial not FM099 EMCS EC 401 000 successful second mission after refurbishment mounted on rotor A in exchange with FM008 no FM001 EMCS WATER RESERVOIR 223 200 successful watering first mission initially mounted on rotor B no successful watering during CWRW exchanged FM009 EMCS WATER RESERVOIR 223 200 with FM002 did not show any failure or deviation from design which could lead to the observed behaviour In addition it could be shown that the RBLSS rotor I F the rotor itself and the rotor EC I F is functional because the returned ECs were wet and the water collected in the filters was visibly clean This rules out a potential blocking of the septum or other parts of this section Dok Nr No EMCS RP 0000 149 DOR x Failure Analysis EADS jo en Report a Ausgabe lssue 1 Datum Date 1 8 2008 Seite Page 4 von of 5 However EMCS RBLSS MODULE FM008 CI 223 100 first mission which had been mounted on EMCS FM rotor B1 B2 and achieved no successful watering there during CWRW showed a major discrepancy during incoming inspection of 1J returned items at KSC The Quick Disconnects QDs towards fresh and waste water reservoir were swapped meaning e QD 10 N Fresh at RBLSS was equipped with a female QD thus fitting into waste QD of the water Reservoir and vice versa e QD 12 N Waste at RBLSS was equipped with a male QD
186. umbia Col O The seed stock was harvested December 20 2007 Production number is 2007 02 The seeds have been treated as follows Genetically modified seeds are generated by transgenic Arabidopsis plants expressing promoter GUS fusion genes Briefly 3 kb 5 upstream regions of AtCesA4 gene was amplified from genomic DNA of A thaliana Col by PCR and cloned in frame upstream of thc GUS gene constructs Jefferson et al 1987 This was followed by transformation into A thaliana Col via Agrobacterium tumefaciens C58 strain using the floral dip transformation technique Clogh et al 1998 The seeds will be received by Motoshi KAMADA JAXA Regards Mrs Professor Kazuhiko NISHITANI Tohoku University Aobayama Aoba ku Sendai Japan 980 8578 Phone 81 22 795 6700 Fax 81 22 795 6669 e mail nishitan mail tains tohoku ac jp N USOC PRO 005 H USOE m CW RW Flight Sample Hardware Packing Transport issue 2 nn and Check Out Procedure Date 27 02 2008 Page 40 of 58 Osaka city University 3 3 138 Sugimoto January 18 2008 Sumisyoshi ku Osaka JAPAN 558 8585 Phone 81 66605 2577 Fax 81 66605 2577 The following information is submitted in support of shipment of seeds by Norwegian University of Science amp Technology NTNU The name of supplier is Professor Takayuki HOSON Osaka city univ Osaka Japan The name of contact person for supplier is Professor Takayuki HOSON The name of the seed is Arabidops
187. uondib seg deis ON deis ET N ES W lans 3unaaoosd dilS Ad dILS 9 AYNGAIONd daLS Ag d s EGTOE Poud 83110837 JO GuLBYNIL 10 BINPSLOUJ SJUDWINS XJ SIW3 mal u223030Jd SV H2310 LOWd JO uoisstaed UAIM ay JNOYJIM ed 184010 Aue Aq Led uj 40 sjoum ur p sol5sip aq zou eus at parjddns sem 3 YIIYM JO asodund at 104 Ajajos 3usjdi2a1 34 Aq pasn aq o3 si pue Sy HDIF 010tid 01 Aleyal daud si uay poutejuon UONRLUJEJUI SYL 3e8us J4jo uBIs e1npaoo4g UDIS pue 3no HU su J10J e1npa204d dags Aq days ubis pue yno mu Bulusiu d uondioseq dejs 6T 40 G i 2Bed 3ungaaoo0 d dils AY dALS EGTOE Pald d 00z S0 0 ep g enss 11 0 Z JO GUY 10 o1npo204d SO ud EGTOL Ad ou 20g Ju sjueuiniedx3 SIW3 u 2303oJd Oud O LINW Ad X3Wa3 ou 5oq Doc no EMEX PR MULT 04 PRO prototech e EMCS Experiments Int Doc no PR 30193 PR 05 Procedure for Filtering of Zeolite Issue B Date 03 05 2007 Project 30193 ACCEPT REJECT CRITERIA Page 15 of 19 7 ACCEPT REJECT CRITERIA The filtering is considered as successful if filtering has been performed according to procedure and no deviations anomalies have occurred 8 DEVIATION All deviations to this procedure shall be recorded on the Procedure Deviation Sheet in Appendix A 9 NONCONFORMANCES All Non Conformance shall be recorded in the Non Conformance List in Appendix B 10 PROCEDURE SIGN OFF SHEET Statement that the Zeolite has be
188. ures on flights to the U S Please contact rose m ogden boeing ksc nasa gov concerning the Duty Free Entitlement Best regards Manfred Nordhoff ISS Product Support ASTRIUM Space Transportation Tel 0421 539 4189 Fax 0421 539 5782 This email including any attachments may contain confidential and or privileged information or information otherwise protected from disclosure If you are not the intended recipient please notify the sender immediately do not copy this message or any attachments and do not use it for any purpose or disclose its content to any person but delete this message and any attachments from your system Astrium disclaims any and all liability if this email transmission was virus corrupted altered or falsified Astrium GmbH Vorsitzender des Aufsichtsrates Thomas Mueller Geschaeftsfuehrung Evert Dudok Vorsitzender Dr Reinhold Lutz Pablo Salame Fischer Guenter Stamerjohanns Sitz der Gesellschaft Muenchen Registergericht Amtsgericht Muenchen HRB Nr 107 647 Weitere Informationen ueber EADS Astrium http www astrium eads net Doc Nr N USOC REQ 025 EMCS CW RW Requirements Issue 1 Integration Report Date 28 11 N USOC A tegratio p ate 28 11 08 Norwegian User Support and Operations Centre Page 1 of 1 Samfunnsforskning AS Appendix 4 CWRW Temperatures logged during transport CWRW Box1 EC092 EC09 EC095 SL52T Standard print printed on fredag 16 mai 2008 at 13 40 55 Logg
189. ve of this set up was to prepare 8 Cell Wall Resist Wall ECs with PCC and seeds for the Cell Wall Resist Wall experiment according to given procedures AD04 and ADO5 N USOC TR 027 CW RW Issue 1 PCC Assembly and Seed Set Up Report Date 13 02 2008 Page 6 of 105 5 Personnel Set up Engineer The set up engineer was responsible for performing set up according to given procedures accuracy of the measured values Set up Manager The set up manager was responsible for set up articles and technical aspects of the set up performance examination of disturbances set up article integration and handling set up performance evaluation of set up results Quality Assurance The an Assurance representative was responsible for surveillance of set up equipment according to regulations as well as the set up procedure application statement that the set up articles to be set up have passed all checks before set up including calibration checking the identification markings on the set up articles supervision of set up proceeding with respect to quality assurance aspects i e correct tracing of procedure variations and non conformances Table 1 Set up personnel Name Affiliation Contact info Responsibility Motoshi Kamada JAXA 81 29 868 3698 Set up Engineer Hakon Svare N USOC 47 735 90162 Set up Engineer Suzanne verlie N USOC 47 735 90181 Set up Manager Maja Rostad N USOC 47 735 9
190. wa panddy DADO WS Te unsmad FWA CAN ans Jo vente cyrus CH SDIEA anjeA SYJELUSM enjoy S9UEJSJOL EUIUION uondwoseq dajs 1saj on dajs3saj 002 9 aqel 95 Jo SZ Seg Z007 90 7 aeg enss inp o5oiq dN I95 pees pue Ajquassy 32d L ua iynyy 1812423 pit eg 200 O8d SOSN N N USOC PRO 002 Multigen 1 PCC Assembly and issue 1 3 Seed Set Up Procedure Date 23 05 2007 Page 26 of 56 9 Procedure Sign off AM steps and activities described in the Procedure have been successfully performed Failures and non conformities raised during procedure execution were listed in table 5 Procedure sign off Engineer Date Quality Assurance Date i je Haa kib 01 felnuuy 2008 Engi hee v Date e G f Dez 200 i z 2 m Haken an Yoz 2008 N USOC PRO 002 AP01 Appendix 1 Issue 1 3 Zeolite Nutrition Enrichment Date 23 05 2007 Page 27 of 56 Appendix 1 Zeolite Nutrition Enrichment N USOC PRO 002 AP01 Inkavatien and Creativity Appendix 1 Issue 1 3 Zeolite Nutrition Enrichment Date 23 05 2007 Page 28 of 56 Prepared by Reference Issue Date of Issue Status Document type Distribution Appendix 1 Zeolite Nutrition Enrichment Suzanne verlie 1 3 23 05 2007 Procedure r eoueunssy AyyenD uoneoo7 aeq q peuuoneg 279 spp vw 2 a80 N Q mm 9 W PBI 23345 sapoed peduinj eyejedes 0 Japio ul ujpiM useui wu u
191. wever sufficient to lead to germination in At April 11 These seedlings were in danger of drying out due to lack of water and therefore workarounds were implemented As a short term plan recycling mode was used water from the dehumidifier was directed to the EC instead of waste and the time period of light was reduced Doc Nr N USOC REQ 025 Issue 1 Date 28 11 08 Page 18 of 30 EMCS CW RW Requirements Integration Report Samfunnsforskning AS N USOC Norwegian User Support and Operations Centre p Crew time was needed again for trying to assess the hydration problems and Garrett performed the Water Reservoir stowage check Even though he confirmed that the correct Water Reservoirs were installed the troubleshooting activities already performed seemed to point out the EMCS Water Reservoirs as the most likely candidates for the hydration issues Therefore on April 22 5 the reservoirs were exchanged with new ones that were stowed on ISS After the reservoir replacement hydration attempts of the ECs was resumed and hydration of position B1 B3 and B4 was deemed successful delta pressure trigger On April 26 and 27 germination was observed in position B3 and B4 Hydration of position A1 A2 A3 A4 and B2 was not successful and the seedlings in A1 were still in danger of drying out Due to the unsuccessful hydration in A1 Crew time was requested again and this time to exchange two ECs on rotor A with two ECs on rotor B Th
192. yatiers nile Wert Social Research Lid pe RE Datod 15 01 2008 Dur ref N USOC NOT SEO 003 Your letter dated Your Ref PROFORMA INVOICE Shippers name Suzanne verlie Address Dragvoll Alle 38 City 7034 Trondheim Country Norway Contact person Suzanne verlie Phone number 47 73 59 01 B1 47 92 09 56 17 Receivers name Transportation officer NASA Address CAPPS warehouse Bldg M8 698 867 2199 Mail Stop SK FL Kennedy Space Centre FL 32899 State Florida Country USA Contact person Thomas Erdman or T Renee Alkinson Phone number 321 867 2188 321 867 6611 Weight Value US Dollar Ep e masi goods Seeds of Arabidopsis Transport Box 1 2 thaliana and 3 Divided in Transport box 1 8 7 kg 308202 SIN FM092 FMO94 FM095 Experiment Transport box 2 8 7 pe Containers SIN FM096 FM098 30 Wild lype seeds Containers 3boxess 243kg 8218728 30 Lefty mutant seeds 30 hmg mutant seeds Experiment Containers 14 GM GUS seeds S N FM099 FM100 Invo e ose only no commercial value no payments Reason for shipment The equipment belongs to the European Space Agency ESA Contract Ref ESTEC Contract 18279 04 NL SH and is transported from Norway Trondheim lo USA FL Kennedy Space Centre for fight of the EMCS Cell Wall Resist Wall Experiment on the Space Shuttle STS 123 1 JIA Country of origin Germany Norway Japan Date March 2008 Signature of shipper gt
193. zi i1e3s pue Buipinol L 96 JO zp abed 2o0z s0 ez a1ea SUEJQUWAN vAd JO uonezijuejs pue Buipinojy enss r xipueddy tDdY Z00 Odd JOSN N soueunssy jjeni uonmeoo ee A0 peuuojiag W V S gd go Io 8010h JO Wou A eye ULM SL Ope uonnjos VAd JO jr OL UMA 2818j84 popusdda apedid asp joo Buipjnojw VAd uo VAd JO Jejdoup ek uonduosaq dee pauuojisg x1euiaM ojoudg uoneisuouleg 96 jo y abed SULIQUSN VAd Jo uoneziji1ajs pue Bulp noy 4002 oU c aed CL anss r xipueddy bOdwW Z00 Otai DOSN N eoueanssy leno uoneoo7 am Aq peuuopeg VAN S g 4 SIOIDEE SOUD A 27000 ura Buuno Jayy sejnuiu OZ 1noqe JO 2 6 je UBAO ui ang enne YJeway ojoyd uoye4jsuousg l uondii5s q dag 95 JO pp abeg 1007 SO tz eier eueJquie y vAd JO uonezijeys pue Burpjnow L enss v xipueddy Aylaledsy pab bheisd FOAV Z00 OUd I0SN N LIN uunjoo WOU SANS eueJquieyy vAd mun spiemdn Ajinjereo Guise uwnjog usnd pue Bur Jsoeds saowsy 92uginssy En UOREI0T sed Aq pauuopad tzv S gg JO ID ET 8070 av ue 46 eUJeIp up G ysig Wed sse e ur aded Jayy esojnjjeoouu Je noJio e uo AjueAe seugiquieyW VAd OZ moge einquisiq Jeouid dn oJotuJ e YIM uuinjo Wou PSAOLISJ 3G UBD pue pajediouews mou SI euelquiey vAd SpJEMUMOP Burseo uwnjo9 usng xc peuuopea yieui su ojoud uonge 3suouu q uonduose

EMCS CW/RW Requirements Integration Report N-USOC-REQ

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