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G1377-90000 - Agilent Technologies

1. Remotecable GPIB or LAN goog oo to LC ChemStation AC power sii AC power Ba popopo B DDDDDODIG AC power E EEEE Analog signal to recorder is AC power Figure 14 Recommended Stack Configuration Thermostatted Autosampler Rear View 1260 Infinity Autosampler User Manual Installing the Autosampler 3 Installing the Autosampler Parts required Preparations Description 1 Sampler Power cord Locate bench space Provide power connections Unpack the sampler Instruments are partially energized when switched off The power supplies still use some power even if the power switch on the front panel is turned off gt To disconnect the thermostatted autosampler from line power unplug the power cord from the autosampler and the ALS thermostat gt Make sure that it is always possible to access the power plug Personal injury To avoid personal injury keep fingers away from the needle area during autosampler operation gt Do not attempt to insert or remove a vial or a plate when the needle is positioned CAUTION De
2. T 2 AC power Analog signal to recorder aa Remote cable i AC power CAN Bus cable AC power AC power Analog signal torecorder ira Ar g a AC power K GPIB or LANto control software Figure 12 Recommended Stack Configuration Well Plate Autosampler Rear View 48 1260 Infinity Autosampler User Manual Installing the Autosampler 3 Optimizing the Stack Configuration Detector Instant Pilot Column Compartment gt a E F Solvent Cabinet Autosampler ay Ey Degasser n I ALS Thermostat Q da Pump t 9J y Figure 13 Recommended Stack Configuration Thermostatted Autosampler Front View 1260 Infinity Autosampler User Manual 49 3 50 Installing the Autosampler ing the otaci ontigurat Analog signal to recorder CAN bus cable DE Autosampler o 5 Thermostat cable
3. tf Agilent Technologies Notices Agilent Technologies Inc 2006 2008 2010 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61377 90000 Edition 06 10 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separ
4. 2 Defective leak sensor 3 Leak sensor incorrectly routed being pinched by a metal component Leak Sensor Short Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause 1 Defective flow sensor 2 Leak sensor incorrectly routed being pinched by a metal component Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative 1260 Infinity Autosampler User Manual Troubleshooting and Diagnostics 6 Compensation Sensor Open The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Plea
5. Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 40 1260 Infinity Autosampler User Manual Site Requirements and Specifications 2 Physical Specifications Table 14 Physical Specifications Type Specification Comments Weight 15 5 kg 35 Ibs Dimensions height x 200 x 345 x 440 mm 8 x 13 5 x 17 inches width x depth Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 300 VA 200 W 683 BTU Maximum Ambient operating 4 55 C 41 131 F temperature Ambient non operating 40 70 C 4 158 F temperature Humidity lt 95 at 25 40 C 77 104 F Non condensing Operating Altitude Up to 2000 m 6562 ft Non operating altitude Up to 4600 m 15091 ft For storing the module Safety standards IEC CSA Installation Category II Pollution Degree 2 For indoor use only UL 1260 Infinity Autosampler User Manual 41 2 Site Requirements and Specifications Performance Specifications Table 15 Performance Specifications Type Specification GLP features Communications Safety features Injection range
6. Valve to Mainpass Failed The injection valve failed to switch to the mainpass position The switching of the injection valve is monitored by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the mainpass position or if the microswitch does not close the error message is generated Probable cause Suggested actions 1 Valve in an intermediate position between Turn the sampler main power OFF and ON the bypass and mainpass positions 2 Defective injection valve Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative 1260 Infinity Autosampler User Manual 103 6 Troubleshooting and Diagnostics Needle Lock Failed The lock assembly on the sampling unit failed to move successfully The upper and lower positions of the needle lock are monitored by position sensors on the sampling unit flex board The sensors detect the successful completion of the needle lock movement If the needle lock fails to reach the end point or if the sensors fail to recognize the needle lock movement the error message is generated Probable cause Suggested actions 1 Defective or dirty position sensor Clean the position sensor 2 Sticking spindle assembly Please contact your Agilent service representative 3 Defective needle drive motor Please contact your Agilent service representative 4 Def
7. 1260 Infinity Autosampler User Manual Contents 6 1260 Infinity Autosampler User Manual 1260 Infinity Autosampler User Manual 1 Introduction Introduction to the Autosampler 8 Sampling Sequence 10 Injection Sequence 12 Sampling Unit 14 Analytical Head 15 Injection Valve 16 Needle Flush Station 16 Needle Lock 17 Needle Sample Transport Assembly 18 Advanced Operating Modes 20 Early Maintenance Feedback EMF 22 Electrical Connections 23 Interfaces 24 Overview Interfaces 26 Setting the 8 bit Configuration Switch On Board LAN 30 Communication Settings for RS 232C 33 Special Settings 35 This chapter gives an introduction to the High Performance Micro Autosampler att Agilent Technologies 7 1 Introduction Introduction to the Autosampler The Agilent 1260 Infinity High Performance Micro Autosampler is designed to perform capillary LC with injection of sample volumes ranging from nL to uL Features A micro Rheodyne valve and the optimized design of the needle seat loop and seat capillaries minimize dispersion A high resolution metering device offers resolution ten times better than a standard autosampler bypass operation facilitates low delay volume increased sample injection speed for high sample throughput flexible and convenient sample handling with different types of sample containers Using 384 well plates allows to process up to 768 samples unattended Technical Principle The well plate sampler transpo
8. Optimizing Performance 73 Optimizing Performance 74 Optimization for Lowest Carry Over 75 Fast Injection Cycle and Low Delay Volume 82 Precise Injection Volume 84 Choice of Rotor Seal 86 Choice of Seat Capillary 87 Troubleshooting and Diagnostics 89 Agilent Lab Advisor Software 90 Overview of the Sampler s Indicators and Test Functions 91 Status Indicators 93 Error Messages 95 Maintenance Functions 112 High Performance Autosampler Step Commands 114 Troubleshooting the Autosampler 117 Errors Which May Occur During the Turn ON and Initialization Process 118 Instrument Logbook Errors and Step by Step Repair Proces 122 Needle Centering Over the Vial or the Well 128 Maintenance 129 Introduction to Maintenance and Repair 130 Maintenance Functions 132 Early Maintenance Feedback EMF 133 Maintenance Procedures 135 Parts and Materials for Maintenance 151 Sampler Main Assemblies 152 Vial Trays 154 Accessory Kits 156 Thermostat for ALS FC Spotter 157 Cable Identification 159 Cable Overview 160 Analog Cables 162 Remote Cables 164 1260 Infinity Autosampler User Manual Contents BCD Cables 167 CAN LAN Cables 169 External Contact Cable 170 RS 232 Cables 171 10 Appendix 173 General Safety Information 174 The Waste Electrical and Electronic Equipment Directive 177 Lithium Batteries Information 178 Radio Interference 179 Sound Emission 180 Use of Solvents 181 Agilent Technologies on Internet 182
9. Precision Pressure range Sample viscosity range Sample capacity Injection cycle time Carry over Early maintenance feedback EMF electronic records of maintenance and errors Controller area network CAN RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas error detection and display 0 01 8 ul in 0 01 pl increments with the small loop capillary 0 01 40 ulin 0 01 pl increments with the extended loop capillary Typically lt 0 5 RSD of peak areas from 5 40 ul Typically lt 1 RSD from 1 5 ul Typically lt 3 RSD from 0 2 1 pl up to 400 bar 5880 psi 0 2 5 cp 2 x well plates MTP 10 x 2 ml vials 108 x 2 mL vials in 2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6 mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials 54 Eppendorf tubes 0 5 1 5 2 0 mL in 2 x 27 Eppendorf tube plate Typically lt 30 s using following standard conditions Default draw speed 4 l min Default eject speed 10 pl min Injection volume 0 1 pl Typically lt 0 05 using the following conditions Column 150 x 0 5 mm Hypersil ODS 3 pm Mobile phase Water Acetonitrile 85 15 Column Flow rate 13 pl min Injection volume 1 pl caffeine 25 ng caffeine 1 ul water to test carryover Outside wash of needle before injection 20 sec with water using flush port 42 1260 Infinity Autosa
10. Rotor Seal on page 143 counter in the EMF is exceeded When the valve performance shows indication of leakage or wear Exchanging the metering seal When autosampler reproducibility indicates See Removing the Metering Seal on seal wear page 144 Exchanging the loop capillary When loop capillary blocked or broken See Removing the Loop Capillary on page 146 Exchanging the peristaltic pump When tubing broken See Peristaltic Pump on page 149 1260 Infinity Autosampler User Manual 135 7 Maintenance Removing the Needle Assembly When Tools required Parts required When the needle is visibly damaged When the needle is blocked Two 1 4 inch 5 16 inch wrenches 8710 0510 supplied in accessory kit 4 mm open end wrench 8710 1534 supplied in accessory kit p n Description 1 G1377 87201 Needle assembly Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be carefull when you remove the needle carrier assembly gt Use the silicon safety tube supplied with every new needle 136 In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found in the Tools icon 2 Open the front door and remove the side door gt on oe ol Remove the plate tray from the tray base Push the silicon safety tube supplied
11. Disconnecting or reconnecting the sampler to thermostat cable when the power cords are connected to either of the two modules will damage the electronics of the modules gt Make sure the power cords are unplugged before disconnecting or reconnecting the sampler to thermostat cable CAUTION Damage through condensation If the condensation tube is located in liquid the condensed water cannot flow out of the tube and the outlet is blocked Any further condensation will then remain in the instrument This may damage the instruments electronics gt Make sure that the condensation tube is always above the liquid level in the vessel ime To avoid personal injury keep fingers away from the needle area during autosampler operation gt Do not attempt to insert or remove a vial or a plate when the needle is positioned 54 1260 Infinity Autosampler User Manual Installing the Autosampler 3 1 Place the thermostat on the bench 2 Remove the front cover and route the condensation drain tube to the waste bottle Condensation drain tube Waste bottle Figure 16 Condensation leak outlet Install the LAN interface board in the sampler if required Remove the adhesive tape which covers the side and front doors Open the front door and remove the left side door Remove the transport protection foam Re install the corrugated waste tube in the plastic port Re install the left sid
12. Please contact your Agilent service representative Exchange the needle carrier assembly Please contact your Agilent service representative Please contact your Agilent service representative No vial was found in the position defined in the method or sequence When the needle carrier moves to a vial and the needle goes into the vial the position of the needle is monitored by an encoder behind the vial pusher If no vial is present the encoder detects an error and the message missing vial is generated Probable cause 1 No vial in the position defined in the method or sequence 2 Defective needle carrier assembly 3 Defective transport assembly flex board 4 Defective MTP board Suggested actions Install the sample vial in the correct position or edit the method or sequence accordingly Exchange the needle carrier assembly Please contact your Agilent service representative Please contact your Agilent service representative 1260 Infinity Autosampler User Manual Initialization Failed Troubleshooting and Diagnostics 6 The autosampler failed to complete initialization correctly The autosampler initialization procedure moves the needle arm and transport assembly to their home positions in a predefined routine During initialization the processor monitors the position sensors and motor encoders to check for correct movement If one or more of the movements is not successful or is not detect
13. Table 3 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote G4212A B DAD 2 No Yes Yes 1 Yes G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 1 Yes G1362A RID G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1316A C TCC 2 No No Yes No Yes G1322A DEG No No No No No Yes AUX G1379B DEG No No No Yes No No AUX G4227A Flex Cube 2 No No No No No G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN e CAN connectors as interface to other modules e LAN connector as interface to the control software RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e Analog output connector s for signal output 1260 Infinity Autosampler User Manual 25 1 26 Introduction Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369A B LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module
14. before the analysis can be continued for example repair exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the instrument log book This section explains the autosampler error messages and provides information on probable causes and suggested actions to recover from error conditions Timeout The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required 1260 Infinity Autosampler User Manual 95 6 Troubleshooting and Diagnostics Shut Down An external instrument has generated a shut down signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down i
15. 2mm hex key 8710 2438 supplied in accessory kit Parts required p n Description 1 G1367 60010 Needle Carrier assembly WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be carefull when you remove the needle carrier assembly gt Use the silicon safety tube supplied with every new needle 1 In the user interface start the maintenance mode and select the Change Needle Carrier function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Carrier function can be found in the Tools icon 2 Open the front door and remove the side door Remove the plate tray from the tray base 4 Push the silicon safety tube supplied in the WPS leak kit G1367 60006 and with every new needle over the needle 5 Pinch the holder clamp pull back and remove the needle assembly from the needle carrier 6 Unplug the flex board on the sample transport 7 Unscrew the three holding hex screws with the 2 mm hex key 8 Remove the needle carrier assy 1260 Infinity Autosampler User Manual 139 7 Maintenance Installing the Needle Carrier Assembly When When the needle carrier is defect Tools required 2mm hex key 8710 2438 supplied in accessory kit Parts required p n Description 1 G1367 60010 Needle Carrier assembly Install a new needle carrier G1367 60010 on place Install the three holding hex screws with the 2 mm hex key Plug in the flex board on the sa
16. 5 Drive the tube from the peristaltic flush pump to the solvent bottle in the solvent cabinet 1260 Infinity Autosampler User Manual Installing the Autosampler 3 Flow Connections to the Sampler 6 Seat capillary see recommendations in Choice of Seat Capillary on page 87 from pump Corrugated tube iK A a Loop capillary waste tube SY ANA azie mm O a l Te gt RTR rA i to waste to column Figure 19 Hydraulic Connections 1260 Infinity Autosampler User Manual 59 3 Installing the Autosampler Installing the Sample Tray 60 Press the bottom on the right side to release the front door Lift the front door Load the sample tray with sample well plates and vials as required Aa Q N Slide the sample tray into the autosampler so that the rear of the sample tray is seated firmly against the rear of the sample tray area 5 Press the front of the sample tray down to secure the tray in the autosampler If the tray pops out of position the air channel adapter is not correctly inserted 1260 Infinity Autosampler User Manual Installing the Autosampler 3 Figure 20 Installing the Sample Tray 1260 Infinity Autosampler User Manual 61 3 Installing the Autosampler Transporting the Sampler 62 When moving the autosampler inside the laboratory no special precautions are needed However if the autosampler needs to b
17. Autosampler User Manual ee 7 e Maintenance J Introduction to Maintenance and Repair 130 Simple Repairs Maintenance 130 Warnings and Cautions 130 Cleaning the Module 131 Maintenance Functions 132 Early Maintenance Feedback EMF 133 EMF Counters 133 Using the EMF Counters 134 Setting the EMF Limits 134 Maintenance Procedures 135 Removing the Needle Assembly 136 Installing the Needle Assembly 137 Removing the Needle Carrier Assembly 139 Installing the Needle Carrier Assembly 140 Exchange the Needle Seat 141 Exchange the Seat Capillary 142 Rotor Seal 143 Metering Seal and Plunger 143 Removing the Loop Capillary 146 Installing the Loop Capillary 148 Peristaltic Pump 149 Installing Interface Board 150 This chapter describes the maintenance of the autosampler gig Agilent Technologies 129 7 Maintenance Introduction to Maintenance and Repair Simple Repairs Maintenance The autosampler is designed for easy repair The most frequent repairs such as changing a needle assembly can be done from the front of the instrument with the instrument in place in the system stack These repairs are described in Maintenance Procedures on page 135 Warnings and Cautions WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for exam
18. Metering Seal pack of 1 for the G1377 60013 40 pl Analytical Head 5064 8293 Micro Plunger assembly In the user interface start the maintenance mode and select the Change Piston function In the Agilent Lab Monitor amp Diagnostic Software the Change Piston function can be found under the Tools icon Open the front door Remove the corrugated leak tubing 4 Remove the two capillaries from the analytical head Use a 1 4 inch wrench oo N OOQ if you have a SST capillary or a 4 mm wrench if you have a fused silica capillary Unscrew alternately the two fixing screws with the 4 mm hex key and remove them Pull the analytical head away from the sampling unit Remove the two fixing screws from the base of the analytical head Remove the head body Using the piston carefully remove the metering seal Clean the chamber and ensure all particular matter is removed 1260 Infinity Autosampler User Manual Maintenance 7 Installing the Metering Seal When When poor injection volume reproducibility When leaking metering device Tools required 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit 4mm 15cm long T handle hex key 8710 2392 supplied in accessory kit Small flat head screwdriver Parts required p n Description 1 5063 6589 Metering seal pack of 2 for 100 pl analytical head 1 5063 6586 Plunger 1 5022 2175 Metering Seal pack of
19. Parity 1 0 Odd Parity 1 1 Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity 1260 Infinity Autosampler User Manual Introduction 1 Special Settings The special settings are required for specific actions normally in a service case The tables include both settings for modules with on board LAN and without on board LAN They are identified as LAN and no LAN Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 12 Boot Resident Settings without on board LAN Mode Select SW1 sw2 SW3 SW4 SW5 SW6 SW7 Swe LAN No LAN TEST BOOT 1 1 1 0 0 0 0 0 TEST BOOT 1 1 0 0 1 0 0 0 CAUTION Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are diagnosis and repair log books which will not be erased gt Save your methods and data before executi
20. X slide carries the antenna and electronics of a RF sensor This device has multiple functions It allows to read and write information from a tag located in the new tray e It allows to increase the number of different trays It allows to read the revision and other data tags of the needle sample transport assembly and sampling unit Complex flex boards make the electrical connection to the various motors sensors and the MTP board The needle carrier has an integrated plate vial pusher with an additional linear encoder to sense vials and the presence of plates The needle and the loop capillary are user exchangeable The back of the needle sample transport assembly has a cover to protect the electronics from potential solvent vapor 1260 Infinity Autosampler User Manual 19 1 Introduction Advanced Operating Modes Multi Draw Mode Optional The multi draw mode provides injection volumes up to 1500 ul In this case a capillary which holds the additional volume is assembled between seat and valve Then the aspirated sample is pushed into the enlarged seat capillary before repetitive aspiration starts After the last aspiration took place the injection valve switches and the mobile phase transports sample towards column Injector Program A sequence of all available single sampling steps can be tailored to customer needs for special applications Injector program capability is offered with the standard instrument Active N
21. away through a waste drain 1260 Infinity Autosampler User Manual Introduction 1 The six port only 5 ports are used injection valve unit is driven by a high speed hybrid stepper motor During the sampling sequence the valve unit bypasses the autosampler and connects flow from the pump to the column directly During injection and analysis the valve unit directs the flow through the autosampler which ensures that all of the sample is injected onto the column and that the metering unit and needle are always free from sample residue before the next sampling sequence begins All the injection valves have different stator heads and different rotor seals The volume of each valve is different Control of the vial plate temperature in the thermostatted autosampler is achieved using an additional Agilent module the Agilent 1290 Infinity Thermostat for ALS FC Spotter The thermostat contains Peltier controlled heat exchangers A fan draws air from the area above the sample vial tray of the autosampler It is then blown through the fins of the cooling heating module There it is cooled or heated according the temperature setting The thermostatted air enters the autosampler through a recess underneath the special designed sample tray The air is then distributed evenly through the sample tray ensuring effective temperature control regardless of how many vials are in the tray In cooling mode condensation is generated on the cooled side of the Pel
22. cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 1260 Infinity Autosampler User Manual 179 10 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 180 1260 Infinity Autosampler User Manual Appendix 10 Use of Solvents Observe the following recommendations on the use of solvents e Brown glass ware can avoid growth of algae e Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric acid and nitric acid especially at higher temperatures Gif your chromatography method allows replace by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCls O gt
23. gt 2COCI 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solvents containing strong complexing agents e g EDTA Mixtures of carbon tetrachloride with 2 propanol or THF 1260 Infinity Autosampler User Manual 181 10 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the modules for download 182 1260 Infinity Autosampler User Manual Index Index 8 bit configuration switch on board LAN 30 without On Board LAN 32 A advanced operating modes 20 Agilent Diagnostic software 90 Agilent Lab Advisor software 90 Agilent Lab Advisor 90 Agilent oninternet 182 algae 181 ambient operating temperature 41 ambient non operating temperature 41 analog signal 27 analog cable 162 apgremote 28 B battery safety information 1 8 BCD cable 167 bench space 40 bypass 12 c cable analog 162 BCD 167 CAN 169 connecting modules 48 external contact 170 LAN 169 remote 164 RS 232 171 cables analog 160 BCD 160 CA
24. in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters The autosampler provides four EMF counters Each counter increments with autosampler use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The autosampler provides the following EMF counters Injection Valve Counter This counter counts the number of valve switching EF4512 since the last reset of the counter Needle Assembly Counter This counter counts the total number of needle into seat movements used for the needle life time EF4510 since the last reset of the counter Seat Assembly Counter This counter counts the total number of needle into seat movements used for the seat life time EF4511 since the last reset of the counter 1260 Infinity Autosampler User Manual 133 7 134 Peristaltic Pump This counter gives the accumulates active pump time in units seconds EF4513 Using the EMF Counters The user setable EMF limits for the EMF counters enable the early maintenance feedback to be adapted to specific user requirements The wear of autosampler components is dependent on the analytical conditions therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF l
25. into the appropriate maintenance position In the ChemStation the sampler maintenance positions can be selected from the Maintenance menu in the Diagnosis display In the Control Module the functions can be selected in the Test screens of the autosampler In the Agilent Lab Monitor amp Diagnostic Software the maintenance positions can be selected from the Tools icon Maintenance Functions The maintenance functions moves the arm assembly in a specific position in order to enables easy access for maintenance operations Home Position The home position function moves the arm to the right side for better access and exchange of the trays Park Position The park position function moves the arm to the left side of the tray In this position it is possible to secure the sample transport mechanism with the protection foam The sample transport is than ready for transporting Change Piston The change piston function draws the piston away from the home position relieving the tension on the spring In this position the analytical head assembly can be removed and reinstalled easily after maintenance This position is also used to change the analytical head plunger and metering seal 1260 Infinity Autosampler User Manual Troubleshooting and Diagnostics 6 Table 27 Maintenance positions Function Arm position in X Arm position in Arm Position in Z Note Theta Change Needle Left side Straight Up No current on Theta Chan
26. module to 3394 6 integrators 35900 60750 Agilent 35900A A D converter 01046 60105 Analog cable BNC to general purpose spade lugs Remote cables p n Description 03394 60600 Agilent module to 3396A Series integrators 3396 Series Il 3395A integrator see details in section Remote Cables on page 164 03396 61010 Agilent module to 3396 Series III 3395B integrators 5061 3378 Agilent module to Agilent 35900 A D converters or HP 1050 1046A 1049A 01046 60201 Agilent module to general purpose BCD cables p n Description 03396 60560 Agilent module to 3396 integrators G1351 81600 Agilent module to general purpose 1260 Infinity Autosampler User Manual Cable Identification 9 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection External Contact Cable p n Description G1103 61611 External contact cable Agilent module interface board to general purposes RS 232 cables p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where th
27. modules are connected by a control cable and both are turned on by the sampler module The thermostat power supply has two externally accessible fuses Damaged electronics Disconnecting or reconnecting the sampler to thermostat cable when the power cords are connected to either of the two modules will damage the electronics of the modules gt Make sure the power cords are unplugged before disconnecting or reconnecting the sampler to thermostat cable Incorrect line voltage at the instrument Shock hazard or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage 38 1260 Infinity Autosampler User Manual CAUTION Site Requirements and Specifications 2 Unaccessable power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified
28. n Description G1375 87317 Seat Capillary 150 mm 0 10 mm ID for G1377 87101 Needle Seat G1375 87316 Seat Capillary 150 mm 0 075 mm ID for G1377 87101 Needle Seat G1375 87300 Seat Capillary 150 mm 0 05 mm ID for G1377 87101 Needle Seat G1377 87101 Needle Seat without capillary for G1377A Disconnect the seat capillary from the injection valve port 5 with the 1 4 5 16 inch wrench Remove the needle seat see Exchange the Needle Seat on page 141 3 Use the seat capillary mounting tool provided in the accessory kit and replace the seat capillary from the seat with the 4 mm wrench Install the seat assembly in its location and reconnect the capillary to the injection valve port 5 Follow the procedure in Exchange the Needle Seat on page 141 to finish the installation Choose the seat capillary diameter as function of the column and the application you run on the system See Choice of Seat Capillary on page 87 1260 Infinity Autosampler User Manual Rotor Seal When Tools required Parts required 10 11 Maintenance 7 When poor injection volume reproducibility When leaking injection valve 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 9 64 inch 15 cm long T handle hex key 8710 2394 supplied in accessory kit p n Description 0100 2088 Vespel Rotor Seal for 0101 1050 injection valve G1377A Open the front door Remove all capillaries from the injection val
29. or smaller This capillary gives full chromatographic performance Due to the small diameter this capillary can show some blockage 1260 Infinity Autosampler User Manual 87 5 Optimizing Performance Choice of Seat Capillary 88 1260 Infinity Autosampler User Manual 1260 Infinity Autosampler User Manual 6 Troubleshooting and Diagnostics Agilent Lab Advisor Software 90 Overview of the Sampler s Indicators and Test Functions 91 Status Indicators 93 Power Supply Indicator 93 Instrument Status Indicator 94 ErrorMessages 95 Maintenance Functions 112 Sample Transport Self Alignment 113 High Performance Autosampler Step Commands 114 Troubleshooting 116 Troubleshooting the Autosampler 117 Errors Which May Occur During the Turn ON and Initialization Process 118 Instrument Logbook Errors and Step by Step Repair Proces 122 Needle Centering Over the Vial or the Well 128 This chapter gives an overview to troubleshooting the High Performance Micro Autosampler ot Agilent Technologies 89 Troubleshooting and Diagnostics Agilent Lab Advisor Software 90 The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all A
30. peristaltic pump to deliver fresh solvent to the wash station The reservoir for the solvent is located in the solvent cabinet the waste is channeled by a separate flex tube to a waste bottle 1260 Infinity Autosampler User Manual Introduction 1 Needle Lock A needle lock is used to support the needle carrier in its function making a firm seal of the needle in its seat The needle lock arm is driven by a stepper motor connected to the spindle assembly by a toothed belt 1260 Infinity Autosampler User Manual 17 Introduction 1 emi ort Ass le Sample Tre Needle Sample Transport Assembly Needle carrier U poy US i iii il X axis Needle assembly Reflective light switches Needle Sample Transport Assembly Figure 6 1260 Infinity Autosampler User Manual 18 Introduction 1 The needle sample transport is a multifunctional module capable of moving the needle into various positions such as different wells in two different plates different vials needle wash position and the needle seat position The active movable axes are the X axis the Z axis and the theta axis the vial plate pusher is an additional passive axis All axes are stepper motor driven and encoder controlled in order to have tight feedback for the axes position The theta and Z axes have spring loaded belt tensioner Reflective light switches detect the presence and type of different trays The
31. returns to the injection port the injection valve is switched to the mainpass position and directs the pump flow back through the sample loop For further information on how to reduce carry over see Optimization for Lowest Carry Over on page 75 Recommended Wash Solvents e water e ethanol e methanol e water acid especially for basic compounds e water base espeecially for acidic compounds e water acetonitrile The life time of the tubing in the peristaltic pump is shortened by the usage of organic solvents 1260 Infinity Autosampler User Manual 79 5 80 Optimizing Performance Cleaning the needle seat If flush port has run out of solvent or the option needle wash hasn t been used for several injections or in case the needle seat has got contaminated the needle seat may be contaminated and carry over is significantly higher than expected For cleaning the needle seat there is an automatic procedure using the mobile phase s solvents If that doesn t work a manual cleaning has to be done the following procedure can be used to clean the needle seat Automatic procedure There s an injector command for flushing the seat So a cleaning method can be set up using an injector program Injector program e INJECT e FLUSH SEAT for 90 0 sec 0 0 mm offset e VALVE mainpass Line 1 starts the run so the pump s time table is started Line 2 let s the needle move above the seat and switches the valve
32. sequence does not exist The reflection sensors on the transport assembly flex board are used to check automatically which sample trays are installed coding on tray If the vial position does not exist in the current sample tray configuration the error message is generated Probable cause Suggested actions 1 Incorrect tray installed Install the correct trays or edit the method or sequence accordingly 2 Incorrect tray definition Install the correct trays or edit the method or sequence accordingly 3 Incorrect vial positions defined in the Install the correct trays or edit the method or method or sequence sequence accordingly 4 Tray recognition defective dirty sample tray Please contact your Agilent service or defective transport assembly flex board representative Peristaltic Pump Error The peristaltic pump motor in the autosampler has failed The current on the motor is used by the MTP board to monitor the speed of the peristaltic pump motor If the current falls below a certain value the error message is generated Probable cause Suggested actions 1 Defective motor Please contact your Agilent service representative 2 Defective SUD board Please contact your Agilent service representative 3 Defective MTP main board Please contact your Agilent service representative 110 1260 Infinity Autosampler User Manual Troubleshooting and Diagnostics 6 Vessel or Wash Vessel Error The needle does not reach the t
33. up steps using the syringe can be skipped steps 5 6 8 1 In the user interface start the maintenance mode and select the Change Loop Capillary function In the Agilent Lab Monitor amp Diagnostic Software the Change Loop Capillary function can be found under the Tools icon Open the front door and remove the side door Remove the plate tray from the tray base Push the silicon safety tube over the needle oF WwW N Remove the corrugated loop capillary waste tube and introduce the small tubing from the leak kit into the loop capillary protection tube 6 Draw up the liquid with the syringe 7 Unlock the needle tighter lock system 8 Draw up the rest of the solvent from the loop capillary protection tube 9 Loosen the loop capillary fitting on the analytical head side 10 Pinch the holder clamp pull back and remove the needle assembly with the loop capillary from the needle carrier 146 1260 Infinity Autosampler User Manual Maintenance 7 11 Attach the 5 16 inch wrench to hold position at the needle assembly Use the 4 mm wrench to loosen the fitting of the loop capillary 12 Pull the loop capillary out from the needle assembly 1260 Infinity Autosampler User Manual 147 7 Maintenance Installing the Loop Capillary When Tools required Parts required 148 Capillary blocked Capillary broken i Two 1 4 inch 5 16 inch wrenches 8710 0510 supplied in accessory kit p n Description G1375 87315
34. valve capillaries 58 Vespel seal 86 vial contents temperature 42 vial numbering 64 vial tray 62 vial racks 8 vials 8 viscous samples 84 84 voltage range 41 WwW waste electrical and electronic equipment 177 WEEE directive 177 weight 41 1260 Infinity Autosampler User Manual 185 www agilent com In This Book This manual contains user information about the Agilent 1260 Infinity High Performance Micro Autosampler The manual describes the following introduction to the sampler e site requirements and specifications e installing configuring and using the autosampler optimizing performance e troubleshooting and diagnostics e maintenance parts and materials e information on cables e safety and legal information Agilent Technologies 2006 2008 2010 Printed in Germany 06 10 G1377 90000 fe Agilent Technologies
35. 0 Blue polypropylene Clear PTFE red rubber p n 5182 3458 Green polypropylene Clear PTFE red rubber p n 5182 3457 Red polypropylene Clear PTFE red rubber p n 5182 3459 Table 24 Screw Caps Description Septa 100 Pack Blue polypropylene Clear PTFE red rubber p n 5182 0717 Green polypropylene Clear PTFE red rubber p n 5182 0718 Red polypropylene Clear PTFE red rubber p n 5182 0719 Blue polypropylene Clear PTFE silicone p n 5182 0720 Green polypropylene Clear PTFE silicone p n 5182 0721 Red polypropylene Clear PTFE silicone p n 5182 0722 68 1260 Infinity Autosampler User Manual Using the Autosampler 4 Configure Well Plate Types If the plate you are using is not found on the List of Recommended Plates and Closing Mat on page 65 you may configure a custom plate Measure the exact dimensions of the plate as marked below and enter the values in the plate configuration table of the ChemStation Figure 22 Well Plate Dimensions straight 1260 Infinity Autosampler User Manual 69 4 Using the Autosampler Configure Well Plate Types Figure 23 Well Plate Dimensions staggered 70 1260 Infinity Autosampler User Manual Using the Autosampler 4 Table 25 Well Plate Dimensions Location Description Definition Limits Rows Number of rows on the plate up to 16 Columns Number of columns on the plate up to 24 Volume Volume in pl of a sample vessel A Row distance Distance in mm between the center of two rows B Col
36. 1 for the G1377 60013 40 pl Analytical Head 1 5064 8293 Micro Plunger assembly 1 Install the new metering seal Press it firmly into position Reassemble the analytical head Press the plunger assembly into the seal Put the two fixing screws in place and reinstall the analytical head to the sampling unit 4 Tighten alternately the two fixing screws with the 4 mm hex key Connect the two capillaries to the analytical head Use a 1 4 inch wrench if you have a SST capillary or a 4 mm wrench if you have a fused silica capillary Reinstall the corrugated leak tubing Close the front door In the user interface close the Change Piston function and exit the maintenance mode In the Agilent Lab Monitor amp Diagnostic Software the Change Piston function can be found under the Tools icon 1260 Infinity Autosampler User Manual 145 7 Maintenance Removing the Loop Capillary When e Capillary blocked Capillary broken Tools required Two 1 4 inch 5 16 inch wrenches 8710 0510 supplied in accessory kit Parts required p n Description 1 G1375 87315 Loop capillary 1 G1377 87300 Loop capillary 40 uL for G1377A WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be careful when you remove the loop capillary gt Use the silicon safety tube for the needle If the loop capillary is not broken and no solvent has leaked into the loop capillary tube the solvent draw
37. 100 Minimum 4 4 Maximum 1000 1000 Micro Well Plate Autosampler with 8 ul loop capillary Default value 4 10 Minimum 0 7 0 7 Maximum 20 100 Micro Well Plate Autosampler with 40 pl loop capillary Default value 4 10 Minimum 0 7 0 7 Maximum 250 250 1260 Infinity Autosampler User Manual 85 5 Optimizing Performance Choice of Rotor Seal 86 Vespel Seal The standard seal has sealing material made of Vespel Vespel is suitable for applications using mobile phases within the pH range of 2 3 to 9 5 which is suitable for the majority of applications However for applications using mobile phases with pH below 2 3 or above 9 5 the Vespel seal may degrade faster leading to reduced seal lifetime Tefzel Seal For mobile phases with pH below 2 3 or above 9 5 or for conditions where the lifetime of the Vespel seal is drastically reduced a seal made of Tefzel is available Tefzel is more resistant than Vespel to extremes of pH however is a slightly softer material Under normal conditions the expected lifetime of the Tefzel seal is shorter than the Vespel seal however Tefzel may have the longer lifetime under more extreme mobile phase conditions PEEK Seal With the High Performance SL Autosampler a PEEK rotorseal is used This warrants a leak tight system at high pressures and allows the usage of solvents ranging from pH 2 3 to 12 The PEEK material may show a reduced lifetime if used with following solvents e Methyl
38. 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 rsvo sys rsvo rsvo Fc The LAN settings are done on the LAN Interface Card G1369A B Refer to the documentation provided with the card Communication Settings for RS 232C The communication protocol used in the column compartment supports only hardware handshake CTS RTR Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the column instrument must be powered up again in order to store the values in the non volatile memory Table 8 Communication Settings for RS 232C Communication without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Bits Parity Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up 1260 Infinity Autosampler User Manual 33 1 34 Introduction Table9 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table 10 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication Table 11 Parity Settings without on board LAN Switches Parity 7 8 0 0 No
39. 377 60023 p n G1377 60013 6 Micro Injection valve assembly for G1377A p n 0101 1050 7 Needle Seat assy for G1377A without capillary p n G1377 87101 Seat cap 0 10 mm ID for G1377 87101 Needle Seat p n G1375 87317 Seat cap 0 075 mm for G1377 87101 Needle Seat p n G1375 87316 Seat cap 0 05 mml for G1377 87101 Needle Seat p n G1375 87300 8 Plate Tray base p n G2258 60011 9 Needle assy for G1377A p n G1377 87201 10 Needle Carrier assembly p n G1367 60010 11 Power supply assembly not visible p n 0950 2528 12 Autosampler Main Board MTP p n G1367 66520 Exchange Assembly MTP board p n G1367 69520 13 Ribbon Cable from ST to MTP p n G1364 81601 Ribbon Cable from SLS to MTP not visible p n G1367 81600 14 Loop capillary for G1377A 8ul injection volume p n G1375 87315 Loop capillary for G1377A 40ul injection volume p n G1377 87300 15 Illumination assembly for sampler p n G1367 60040 Sampler TCC cap 500 mm 0 05 mm id for G1377 78A Fan not visible Fan exhaust not visible BCD board not visible p n G1375 87304 p n 3160 1017 p n 3160 4097 p n G1351 68701 1260 Infinity Autosampler User Manual 153 8 Parts and Materials for Maintenance Vial Trays Vial Trays Table 32 Autosampler Vial Trays and Tray Base Item Description Part Number 1 Tray for 2 plates 10 x 2 ml vials p n G2258 60011 2 Screws for springs p n 0515 0866 3 Spring p n G1313 09101 4 Spring stud p n 0570 1574 5 Tray base includes item
40. 5003 Torque adapter G1367 60006 WPS Leak Kit G1375 87304 Fused silica PEEK capillary 50 pm 50 cm G1375 87316 Seat Capillary 150 mm 0 075 mm ID for G1377 87101 Needle Seat G1329 43200 Adapter air channel 5181 1519 CAN cable Agilent module to module 1 m 8710 1534 Wrench 4 mm both ends open end G1377 44900 tool for Micro Seat Capillary Mounting G1377 87300 Loop capillary 40 uL for G1377A 156 1260 Infinity Autosampler User Manual Parts and Materials for Maintenance 8 Thermostat for ALS FC Spotter Thermostat for ALS FC Spotter Table 35 Thermostat for ALC FC Spotter Item Description Part Number 1 Thermostat exchange assembly p n G1330 69040 Figure 28 Thermostat for ALS FC Spotter 1260 Infinity Autosampler User Manual 157 8 Parts and Materials for Maintenance Thermostat for ALS FC Spotter 158 1260 Infinity Autosampler User Manual 1260 Infinity Autosampler User Manual 9 Cable Identification Cable Overview 160 Analog Cables 162 Remote Cables 164 BCD Cables 167 CAN LAN Cables 169 External Contact Cable 170 RS 232 Cables 171 This chapter provides information on cables used with the Agilent 1260 Infinity modules gg Agilent Technologies 159 9 Cable Identification Cable Overview 160 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n Description 35900 60750 Agilent
41. BCD 4 10 5 5 BCDO 1 1e 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 168 1260 Infinity Autosampler User Manual Cable Identification 9 CAN LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN Cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection 1260 Infinity Autosampler User Manual 169 9 Cable Identification External Contact Cable 5o C G 1 100 lt 6 ie e e11 One end of this cable provides a 15 pin plug to be connected to Agilent modules interface board The other end is for general purpose Agilent Module Interface Board to general purposes External contact cable Color Pin Agilent Signal Name Agilent module interface module board to general purposes p n G1103 61611 White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 170 1260 Infinity Autosampler User M
42. Digital ground a NC 2 Brown Prepare run Low 3 3 Gray Start Low z NC 4 Blue Shut down Low 5 NC 5 Pink Not connected i NC 6 Yellow Power on High o 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators p n 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 1260 Infinity Autosampler User Manual Cable Identification 9 Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground gt NC 2 Brown Prepare run Low e 3 3 Gray Start Low m s NC 4 Blue Shut down Low e O a NC 5 Pink Not connected NC 6 Yellow Power on High o 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 A D Pin Agilent Signal Name Active module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 1260
43. Infinity Autosampler User Manual 165 9 Cable Identification Agilent Module to General Purpose p n 01046 60201 Pin Universal Pin Agilent Signal Name Active module TTL 1 White Digital ground A O 1 2 B oo Brown Prepare run Low o0 oo og 3 Gray Start Low KEY coal oo BG 4 Blue Shut down Low o0 am 5 Pink Not connected oOo 0 ji 6 Yellow Power on High O 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 166 1260 Infinity Autosampler User Manual BCD Cables Cable Identification 9 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 Violet 2 BCD7 80 4 Blue 3 BCD 6 40 m Yellow 4 BCD 4 10 SS Black 5 BCD 0 1 ay Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low 1260 Infinity Autosampler User Manual 167 9 Cable Identification Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module 1 1 BCD 5 20 2 2 BCD7 80 8e 7 3 3 BCD6 40 4 4
44. Loop capillary G1377 87300 Loop capillary 40 uL for G1377A Pull the new loop capillary in the needle assembly Attach the 5 16 inch wrench to hold position at the needle assembly Use the second wrench to tighten the loop capillary fitting Push the loop capillary into the loop capillary protection tube until it comes out on the sampling unit side 4 Reinstall the loop capillary corrugated waste tube over the loop capillary 5 Retighten the loop capillary fitting on the analytical head 6 Pinch the holder clamp and reinsert the needle assembly into the needle carrier 7 Push the black chain into the needle assembly until the stop 8 Lock the needle tighter lock system Check the alignment of the needle in the needle pusher of the needle carrier by viewing from several directions to see that it is aligned in the center of the needle pusher The needle must be centered in the needle pusher as all alignment by the autosampler is calculated from the needle pusher position 10 Remove the silicon safety tube from the needle 11 Replace the plate tray in the tray base Re install the side door and close the front door 12 In the user interface close the Change Loop Capillary function and exit themaintenance mode In the Agilent Lab Monitor amp Diagnostic Software the Change Loop Capillary function can be found under the Tools icon 1260 Infinity Autosampler User Manual Maintenance 7 Peristaltic Pump When
45. N 161 external contact 161 LAN 161 overview 160 remote 160 RS 232 161 CAN cable 169 capillaries 58 choice of vials and caps 84 cleaning 131 Communication settings RS 232C 33 compensation sensor open 99 compensation sensor short 99 condensation 40 D damaged packaging 44 delivery checklist 44 44 Diagnostic software 90 dimensions 41 draw speed 84 84 DRAW 84 1260 Infinity Autosampler User Manual E ejectspeed 84 84 EJECT 84 electronic waste 177 EMF counters 133 setting 134 using 134 environment 38 error messages fan failed 100 error messages arm movement 102 compensation sensor open 99 compensation sensor short 99 front door error 101 initialization failed 107 invalid vial position 110 leak sensor open 98 leak sensor short 98 leak 97 metering home failed 108 missing vial 106 motor temperature 109 needle lock failed 104 needle to needle seat position 105 peristaltic pump error 110 remote timeout 96 shut down 96 synchronization lost 97 timeout 95 valve to bypass failed 103 valve to mainpass failed 103 vessel error 111 vessel stuck to needle 111 external contact 183 Index cable 1 0 F failure 91 fan failed 100 flow connections 58 frequency range 41 fuses 38 H humidity 41 injection valve 16 injection volume precision 84 injection volumes less than 2 ul 84 injection sequence 12 injection valve 8 14 injection volume 84 injection volumes 84 installation ben
46. Pack Using the Autosampler 1000 Pack 100 Pack silanized 4 Description Volume ml Clear glass 2 Clear glass 2 write on spot Amber glass 2 write on spot p n 5181 3375 p n 5182 0543 p n 5182 3376 p n 5183 4491 p n 5183 4492 p n 5183 4493 p n 5183 4494 p n 5183 4495 Table 20 SnapTop Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 p n 5182 0544 p n 5183 4504 p n 5183 4507 Clear glass 2 p n 5182 0546 p n 5183 4505 p n 5183 4508 write on spot Amber glass 2 write on spot p n 5182 0545 p n 5183 4506 p n 5183 4509 100 Pack 1000 Pack 100 Pack silanized Table 21 Screw Top Vials Description Volume ml Clear glass 2 Clear glass 2 write on spot Amber glass 2 write on spot p n 5182 0714 p n 5182 0715 p n 5182 0716 p n 5183 2067 p n 5183 2068 p n 5183 2069 p n 5183 2070 p n 5183 2071 p n 5183 2072 1260 Infinity Autosampler User Manual 67 4 Using the Autosampler Table 22 Crimp Caps Description Septa 100 Pack Silver aluminum Clear PTFE red rubber p n 5181 1210 Silver aluminum Clear PTFE red rubber p n 5183 4498 1000 Pack Blue aluminum Clear PTFE red rubber p n 5181 1215 Green aluminum Clear PTFE red rubber p n 5181 1216 Red aluminum Clear PTFE red rubber p n 5181 1217 Table 23 Snap Caps Description Septa 100 Pack Clear polypropylene Clear PTFE red rubber p n 5182 055
47. TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal 1260 Infinity Autosampler User Manual Introduction 1 Table5 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces Some modules have module specific interfaces connectors They are described in the module documentation 1260 Infinity Autosampler User Manual 29 1 Introduction Setting the 8 bit Configuration Switch On B
48. Tools required Parts required Tubing blocked or broken sand paper p n Description 1 5065 4445 Peristaltic pump with Pharmed tubing The peristaltic pump is a replaceable unit The tubing inside the pump is not replaceable FP O N Remove the corrugated leak tubing Press the two clips on the front of the peristaltic pump Pull the pump forward off the motor shaft Disconnect the tubing leading to the wash port and the tubing coming from the solvent bottle Connect the wash port tubing to the upper tubing of the new pump use sandpaper to get a good grip on the tubing Connect the tubing coming from the solvent bottle to the lower tubing of the new pump 7 Push the pump onto the motor shaft until the clips click into place 8 Reinstall the corrugated leak tubing 1260 Infinity Autosampler User Manual 149 7 Maintenance Installing Interface Board When For all repairs inside the sampler or for installation of the board Tools required Flat head screwdriver Parts required Description 1 Interface board CAUTION Electrostatic discharge at the interface board The interface board is sensitive to electrostatic discharge gt Always use the ESD strap when handling electronic boards 1 Switch off the autosampler at the main power switch 2 Disconnect all cables from the existing interface board Then loosen the interface board holding screws and slide the board out of its holding rails 3 Identify the in
49. Up Needle into Seat Action Switches injection valve to the bypass position Moves the plunger to the home position Lifts the needle arm to the upper position Move the needle arm to the vial location on the plate Lowers the needle into the vial Metering device draws the defined injection volume Lifts the needle out of the vial Lowers the needle arm into the seat Comments Command also switches the valve to bypass if it is not already in that position Command lifts the needle and lowers the needle into sample Command can be done more than once maximum draw volume of 40 100 5000 ul cannot be exceeded Use Plunger Home to reset the metering device 1260 Infinity Autosampler User Manual Troubleshooting and Diagnostics 6 Table 28 Step Commands Step Action Comments Valve Mainpass Switches the injection valve to the mainpass position Needle Up Mainpass Lifts the needle arm to the upper position and Switches the injection valve to the mainpass position 1260 Infinity Autosampler User Manual 115 6 Troubleshooting and Diagnostics Troubleshooting If the autosampler is unable to perform a specific step due to a hardware failure an error message is generated You can use the step commands to perform an injection sequence and observe how the autosampler responds to each command Table 29 on page 116 summarizes the step commands and lists the error messages and probable causes associated
50. al Accessory Kits p n 5063 6527 9222 0518 G1315 45003 G1367 60006 G1375 87304 G1375 87316 G1329 43200 5181 1519 8710 1534 G1377 44900 G1377 87300 Installing the Autosampler Description Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste Bag plastics Torque adapter WPS Leak Kit Fused silica PEEK capillary 50 pm 50 cm Seat Capillary 150 mm 0 075 mm ID for G1377 87101 Needle Seat Adapter air channel CAN cable Agilent module to module 1 m Wrench 4 mm both ends open end tool for Micro Seat Capillary Mounting Loop capillary 40 uL for G1377A 1260 Infinity Autosampler User Manual 3 45 3 Installing the Autosampler Optimizing the Stack Configuration 46 If your autosampler is part of a system you can ensure optimum performance ensuring minimum delay volume by installing the following configuration Figure 11 on page 47 and Figure 12 on page 48 show the configuration recommended for the sampler Figure 13 on page 49 and Figure 14 on page 50 show the configuration recommended for the thermostatted sampler 1260 Infinity Autosampler User Manual Installing the Autosampler i Solvent cabinet Vacuum degasser Instant Pilot Autosampler Column compartment Detector Figure 11 Recommended Stack Configuration Well Plate Autosampler Front View 1260 Infinity Autosampler User Manual 3 47 3 Installing the Autosampler
51. anual Cable Identification 9 RS 232 Cables p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m 1260 Infinity Autosampler User Manual 171 9 Cable Identification RS 232 Cables 172 1260 Infinity Autosampler User Manual This appendix provides general safety and environmental information 1260 Infinity Autosampler User Manual 10 Appendix General Safety Information 174 The Waste Electrical and Electronic Equipment Directive 177 Lithium Batteries Information 178 Radio Interference 179 Sound Emission 180 Use of Solvents 181 Agilent Technologies on Internet 182 ee Agilent Technologies 173 10 Appendix General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Ensure the proper usage of the equipment The protection
52. arget position in the vial or in the vessel of the well plate The sensor behind the vial pusher in the needle carrier assembly detects the successful completion of the needle movement to the vessel If the needle fails to reach the end point the sensor fails to recognize the needle movement and the error message is generated Probable cause Suggested actions 1 Bad vessel definition in the plate Check the vessel definition in the plate configuration configuration 2 Closing mat to rigid thick Check that the closing mats is not too thick 3 Bad X or Theta positioning Exchange the needle carrier assembly 4 Defective encoder on the needle carrier Please contact your Agilent service assembly representative Vessel Stuck to Needle The vessel sticks to the needle when the needle moves up Probable cause Suggested actions 1 Closing mat to rigid thick Check that the closing mat is not too thick 2 Bad X or Theta positioning and the needle Exchange the needle carrier assembly sticks into the wall between two holes 3 Defective encoder on the needle carrier Please contact your Agilent service assembly representative 1260 Infinity Autosampler User Manual 111 6 Troubleshooting and Diagnostics Maintenance Functions 112 Some maintenance procedures require the needle arm metering device and needle carrier to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies
53. ate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could resul
54. ch space 40 installing the autosampler flow connections 58 sample trays 64 installing the sample tray 60 installing the thermostatted autosampler preparation 55 installing the thermostatted autosampler interface cables 54 safety 51 54 tray cover and front cover 62 installing the autosampler interface cables 51 safety 51 installing the autosampler 51 power cable 51 installing the thermostatted autosampler 184 power cable 54 instrument status indicator 94 interfaces 24 internet 182 introduction to the autosampler 8 L LAN cable 169 leak sensor open 98 leak sensor short 98 leak 97 line frequency 41 line voltage 41 lithium batteries 178 low volume injections 84 M mainpass 12 maintenance functions 91 112 maintenance procedures 135 maintenance functions 132 step commands 114 maintenance procedures 22 message remote timeout 96 metering device 84 metering device 14 missing parts 44 multi draw option 8 needle flush station 16 needle lock 17 needle drive 14 needle sample transport assembly 18 non operating altitude 41 Non operating temperature 41 numbering of vials 64 0 operating Altitude 41 operating modes 20 operating temperature 41 optimizing performance automated needle wash 84 delay volume 84 delay volume adjustment 84 early maintenance 84 inject valve seal 84 low volume capillary kit 84 P park transport assembly 62 parts and materials 44 vial trays and tray bas
55. d Perform an auto alignment Check the connector from the needle carrier to the sample transport unit Check the sample transport connectors on the main board sample transport Check the connector on the bottom of the sample transport unit Change the needle and the seat Change the needle carrier assembly G1367 60010 Change the sample transport unit Change the main board G1367 69520 1260 Infinity Autosampler User Manual 127 6 Troubleshooting and Diagnostics Needle Centering Over the Vial or the Well 128 The positioning of the needle is very precise You have to take no action if the needle hits in the safe area metal border Septum Safe area to hit Figure 25 Vial cap If the diameter for the safe area is approximately 7mm smaller than the diameter of the septum no corrective action is necessary Action to Take if the Needle Does Not Hit in the Safe Area V Check if the right vials or plates are used see or see List of Recommended Vials and Caps on page 67 V Make sure the needle is correctly installed It should be pushed into the needle carrier as far forward as possible and centered in the vial pusher V Upgrade the firmware revision to A 04 14 or higher and the ChemStation revision to A 08 04 or higher Y Perform an auto alignment without any plates in place V Change the tray G2258 60011 see service note G1367 007 1260 Infinity Autosampler User Manual 1260 Infinity
56. dle is moved into the wash port after the sample is drawn By washing the needle after drawing a sample the sample is removed from the outer surface of the needle immediately As the flush port is automatically refilled with fresh wash solvent this option should be used routinely Using wash vials is usually not necessary but available for special applications Using a wash vial If a wash vial is used it should be considered not to cap the vial Otherwise small amounts of sample remain on the surface of the septum which may be carried on the needle to the next sample 1260 Infinity Autosampler User Manual Optimizing Performance 5 Using the Flush Port During the injection process when the sample is in the loop and when the valve still is in Bypass the outside of the needle can be washed in a flush port located behind the injection port on the sampling unit During the wash cycle as soon the needle is in the flush port a peristaltic pump fills the flush port with fresh solvent during a defined time The volume of the flush port is about 680 ul and the pump delivers 6 ml min Setting the wash time to 10 seconds is sufficient to refill 2 times the flush port In most cases this is enough to clean the needle outside Additionally after the needle left the flush port the flush pump keeps running runs for 6 sec to ensure refill with fresh solvent At the end of this flush process if injection with needle wash is selected the needle
57. duct is classed as a Monitoring and Control Instrumentation product s Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1260 Infinity Autosampler User Manual 177 10 Appendix Lithium Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren 178 Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 1260 Infinity Autosampler User Manual Appendix 10 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All
58. e 154 parts and materials transport assembly 152 Peek seal 86 performance specifications 42 physical specifications 41 power supply indicator 93 Power Consideration 38 power consumption 41 power cords 39 power requirements 38 R radio interference 179 remote cable 164 repairs maintenance procedures 135 metering plunger 143 metering seal 143 needle assembly 136 rotor seal 143 1260 Infinity Autosampler User Manual Index rotor seal 86 RS 232C cable 171 communication settings 33 S safety class 174 safety information lithium batteries 178 safety general information 174 standards 41 symbols 176 sample trays 64 numbering of vial positions 65 sampling sequence 10 sampling unit 14 seals 86 144 145 shipping containers 44 shipping 62 shut down 96 site requirements 38 power cords 39 solvents 181 sound emission 180 special interfaces 29 special settings boot resident 35 forced cold start 35 specification physical 41 specifications 42 stack configuration rearview 48 rearview 50 thermostatted autosampler 50 stator 16 status indicator 91 status indicators 93 step functions 92 X step commands 114 Xais 18 synchronization lost 97 Z T Z axis 18 Tefzel seal 86 temperature sensor 97 temperature 42 thermostat 157 theta axis 18 timeout 95 transport assembly 18 transport mechanism 8 transport 62 transporting the autosampler 62 tray combinations 65 trays 64 U unpacking the autosampler 44 V
59. e door take care of the magnet at the back soo N OO 1 FP Q Place the sampler on top of the thermostat Make sure that the sampler is correctly engaged in the thermostat locks 1260 Infinity Autosampler User Manual 55 3 Installing the Autosampler 10 Remove the tray and the plastic cover from the tray base place the air channel adapter into the sampler tray base Make sure the adapter is fully pressed down This assures that the cold airstream from the thermostat is correctly guided to the tray area of the well plate sampler Air channel adapter Figure 17 Installation of Thermostat and Autosampler 11 Re install the tray 12 Ensure the power switch on the front of the sampler is OFF and the power cables are disconnected 13 Connect the cable between the sampler and the thermostat see Figure 18 on page 57 14 Connect the power cables to the power connectors 15 Connect the CAN cable to other Agilent modules 16 If a Agilent ChemStation is the controller connect the LAN connection to the LAN interface 17 Connect the APG remote cable optional for non Agilent 1200 Infinity Series instruments 18 Ensure the side panel is correctly installed 19 Turn ON power by pushing the button at the lower left hand side of the sampler 56 1260 Infinity Autosampler User Manual Installing the Autosampler 3 20 Close the front door The exhaust fan will turn ON and remove the vapor from the tray compartment After 1 2 min
60. e indicates initializing during this period 4 The vapor blowout period begins This lasts for about 2 minutes 5 WPS sample transport and sampling unit initialization begins at the 2 minutes mark from turn on if the front cover is closed If the front cover is open at the 2 minutes mark initialization will start only when the front cover is closed Initialization takes about 1 5 minutes When initialization is complete the needle is in the needle seat the needle lock is down and the status indicator is off StatusLED ai Power LED Figure 24 Instrument LED indicator 72 1260 Infinity Autosampler User Manual 000 J e ol This chapter provides information on how to optimize the autosampler 1260 Infinity Autosampler User Manual Optimizing Performance Optimizing Performance 74 Optimization for Lowest Carry Over 75 Using the Automated Needle Wash 78 Using the Flush Port 79 Cleaning the needle seat 80 Fast Injection Cycle and Low Delay Volume 82 Precise Injection Volume 84 Draw and Eject Speed 84 Choice of Rotor Seal 86 Choice of Seat Capillary 87 ee Agilent Technologies 73 5 Optimizing Performance Optimizing Performance 74 Autosamplers are more and more used in HPLC to improve the productivity in the laboratories and the consistency and accuracy of analytical results The informations below will help you on how to optimize some parameters to achieve best results for lowest ca
61. e shipped to another location via carrier ensure e The transport assembly is in the park position Use the Lab Monitor and Diagnostic software or the Instant Pilot for this command The vial tray and the sample transport mechanism is secured with the transport protection foam 1260 Infinity Autosampler User Manual 1260 Infinity Autosampler User Manual 4 Using the Autosampler Sample Trays 64 List of Recommended Plates and Closing Mat 65 List of Recommended Vials and Caps 67 Configure Well Plate Types 69 Turn ON and Initialization Steps 72 This chapter describes the usage of the High Performance Micro Autosampler att Agilent Technologies 63 4 Using the Autosampler Sample Trays Supported Trays for an Autosampler Table 16 Trays for an Autosampler G2258 60011 Tray for 2 well plates or vial plates and 10 x 2 ml vials Pos P2 A1 Cc Vial 10 Pos P2 B1 fe ISHII Plate 2 HOOO08 Pos P2 P24 Pos P1 A1 Pos P1 B1 S Plate 1 Pos P1 H12 Vial 1 I Figure 21 Numbering of vial and well plate position 64 1260 Infinity Autosampler User Manual Using the Autosampler List of Recommended Plates and Closing Mat WARNING Explosive gas mixtures There is a risk of building explosive gas mixtures in the instrument if flammable solvents are used gt Cover the plates gt Remove the plates from the sampler after turning it OFF 4 WARNING Contamination with adhesives Closing mats with adh
62. e wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m 1260 Infinity Autosampler User Manual 161 9 Cable Identification Analog Cables 162 One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog E57 1260 Infinity Autosampler User Manual Agilent Module to BNC Connector Cable Identification 9 p n 8120 1840 Pin BNC Pin Agilent Signal Name module nn dl Shield Shield Analog lt _ EO Center Center Analog Agilent Module to General Purpose p n 01046 60105 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Black Analog 3 Red Analog E xg 1260 Infinity Autosampler User Manual 163 9 Cable Identification Remote Cables 164 O O JNE One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3394 Pin Agilent Signal Name Active module TTL Z 9 1 White
63. eat Capillary 150 mm 0 075 mm ID for G1377 87101 Needle Seat G1375 87300 Seat Capillary 150 mm 0 05 mm ID for G1377 87101 Needle Seat In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the change needle seat function can be found under the Tools icon Open the front door and remove the side door Remove the plate tray from the tray base Disconnect the seat capillary from the needle seat with the 4 mm open wrench Use the flat head screwdriver to remove the needle seat Insert the new needle seat Press it firmly in position Connect the seat capillary to the needle seat with the 4 mm open wrench Replace the plate tray in the tray base Re install the side door and close the front door In the user interface close the Change Needle Seat function and exit the maintenance mode In the Agilent Lab Monitor amp Diagnostic Software the change needle seat function can be found under the Tools icon The seat capillary can be exchanged alone if the needle seat is not damaged 1260 Infinity Autosampler User Manual 141 7 Maintenance Exchange the Seat Capillary When Tools required Parts required 142 When the seat is visibly damaged When the seat capillary is blocked 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4 mm open end wrench 8710 1534 supplied in accessory kit Flat screwdriver p
64. ecognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system Leak A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 1260 Infinity Autosampler User Manual 97 98 Troubleshooting and Diagnostics Leak Sensor Open The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause 1 Leak sensor not connected to the main board
65. ective main board Please contact your Agilent service representative 104 1260 Infinity Autosampler User Manual Needle to Needle Seat Position Troubleshooting and Diagnostics The needle failed to reach the end position in the needle seat The position of the needle is monitored by a position encoder on the needle carrier If the needle fails to reach the end point or if the encoder fails to recognize the needle carrier movement the error message is generated Probable cause 1 Bad sample transport sampling unit alignment 2 Bent needle 3 Missing needle 4 Blocked seat 5 Defective position sensor in the needle carrier assembly 6 Defective MTP board 1260 Infinity Autosampler User Manual Suggested actions Do an auto alignment Check and exchange the needle assembly if necessary Clean or change the needle seat assembly if necessary Exchange the needle carrier assembly Please contact your Agilent service representative Please contact your Agilent service representative 6 105 106 Troubleshooting and Diagnostics Needle Carrier Failed The needle carrier on the Sample Transport Assembly failed to move correctly Probable cause 1 Defective Z motor 2 Vial pusher blocked 3 Bad needle carrier positioning in X or Theta 4 Defective vial pusher sensor 5 Defective MTP main board Missing Vial or Missing Wash Vial Suggested actions Exchange the needle carrier assembly
66. ed the error message is generated Probable cause 1 Side door not installed correctly 2 Sample transport sampling unit not aligned correctly 3 Mechanical obstruction 4 Defective sampling unit flex board 5 Defective transport assembly flex board 6 Defective sampling unit motor 7 Defective MTP main board 1260 Infinity Autosampler User Manual Suggested actions Check if the side door is installed correctly Check if the magnet is in place in the side door Do an auto alignment Ensure unobstructed movement of the transport assembly Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative 107 6 Troubleshooting and Diagnostics Metering Home Failed The metering plunger has failed to move back to the home position The home position sensor on the sampling unit flex board monitors the home position of the plunger If the plunger fails to move to the home position or if the sensor fails to recognize the plunger position the error message is generated Probable cause Suggested actions 1 Dirty or defective sensor Exchange the sampling unit flex board 2 Broken plunger Exchange the metering plunger and seal 3 Defective metering drive motor Please contact your Agilent service representative 4 Defective MTP board Please contact your Agil
67. eedle Wash The active needle wash mode allows also the flushing of the outer surface of the needle This results in an additional decrease of sample carry over Duration of the procedure is setable Overlap Injection Cycle Overlapped injection is the mode where the autosampler runs the injector program for the next analysis during the current analysis without injecting After the sample has reached the column the valve is switched back to bypass and the next injection cycle starts but waits with switching to main pass until the actual run is finished This mode allows it to increase the sample throughput 20 1260 Infinity Autosampler User Manual Introduction 1 Low Delay Volume Mode This mode is especially interesting for gradient elution with small bore or capillary columns The injection valve is switched back to bypass after the sample is eluted beyond the injection valve port 6 This decreases the delay volume because the gradient needs not to pass the metering device and the loop capillary 1260 Infinity Autosampler User Manual 21 1 Introduction Early Maintenance Feedback EMF 22 The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user settable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled For details on EMF counters and how to use
68. enance functions position the needle assembly the needle carrier the sample transport assembly and the metering device for easy access when doing maintenance see Maintenance Functions on page 112 Sample Transport Self Alignment The sample transport self alignment with the sampling unit and the well plate tray is required to compensate for larger deviations in positioning the needle carrier The sample transport self alignment is required after disassembling the system or when you exchange the sample transport the sampling unit the tray or the MTP main board This function is in the diagnose screen of the Chemstation or the Control Module 1260 Infinity Autosampler User Manual 91 6 Troubleshooting and Diagnostics Step Commands The step functions enable execution of each step of the sampling sequence individually The step functions are used primarily for troubleshooting and for verification of correct autosampler operation after repair see High Performance Autosampler Step Commands on page 114 For details on step commands please refer to the Agilent Lab Monitor amp Diagnostic Software 92 1260 Infinity Autosampler User Manual Troubleshooting and Diagnostics 6 Status Indicators Two status indicators are located on the front of the autosampler The lower left indicates the power supply status the upper right indicates the autosampler status Status indicator green yellow red Line powe
69. ene chloride e DMSO gt THF e High concentrations of sulfuric acid e High concentrations of nitric acid 1260 Infinity Autosampler User Manual Optimizing Performance 5 Choice of Seat Capillary The needle seat assembly is made up of two parts needle seat and seat capillary Different models of seat capillaries are available p n Description G1377 87101 Needle Seat without capillary for G1377A G1375 87317 Seat Capillary 150 mm 0 10 mm ID for G1377 87101 Needle Seat G1375 87316 Seat Capillary 150 mm 0 075 mm ID for G1377 87101 Needle Seat G1375 87300 Seat Capillary 150 mm 0 05 mm ID for G1377 87101 Needle Seat Seat Capillary 150 mm 0 10 mm ID for G1377 87101 Needle Seat p n G1375 87317 is the capillary preinstalled in the micro well plate autosamplers upon delivery This capillary is recommended for applications with a 0 3 mm column or higher It provides less plugging of the capillary in general and especially with biological samples For small K this capillary can provide a higher peak width for isocratic analysis Seat Capillary 150 mm 0 075 mm ID for G1377 87101 Needle Seat p n G1375 87316 is available as a spare part and is recommended for applications with a 0 3 mm column or smaller This capillary gives full chromatographic performance Seat Capillary 150 mm 0 05 mm ID for G1377 87101 Needle Seat p n G1375 87300 is available as a spare part and is recommended for applications with a 0 3 mm column
70. ent service representative 108 1260 Infinity Autosampler User Manual Troubleshooting and Diagnostics 6 Motor Temperature One of the motors of the transport assembly has drawn excessive current causing the motor to become too hot The processor has switched off the motor to prevent damage to the motor See figure for motor identification e Motor 0 temperature X axis motor e Motor 2 temperature Theta gripper rotation motor Motor 1 temperature Z axis motor The processor monitors the current drawn by each motor and the time the motor is drawing current The current drawn by the motors is dependent on the load on each motor friction mass of components etc If the current drawn is too high or the time the motor draws current is too long the error message is generated Probable cause Suggested actions 1 Mechanical obstruction Ensure unobstructed movement of the transport assembly 2 High friction in the transport assembly Please contact your Agilent service representative 3 Motor belt tension too high Switch off the autosampler at the power switch Wait at least 10 minutes before switching on again 4 Defective motor Please contact your Agilent service representative 5 Defective transport assembly flex board Please contact your Agilent service representative 1260 Infinity Autosampler User Manual 109 6 Troubleshooting and Diagnostics Invalid Vial Position The vial position defined in the method or
71. ent to position 0 Turn on the system Check the connector from needle carrier to the sample transport unit Check the sample transport connectors on the main board sample transport Check the connector on the bottom of the sample transport unit Change the needle carrier assembly G1367 60010 Change the sample transport unit Change the main board G1367 69520 125 6 126 Troubleshooting and Diagnostics 7 Needle Seat Error Probable cause 1 Suggested actions Upgrade the firmware revision to A 04 14 or higher and the ChemStation revision to A 08 04 or higher Check if the needle is installed the sample transport comes without needle Check the needle position and correct alignment in the pusher Check if the seat is not blocked with any parts or material crystals glass Perform an auto alignment Check the connector from the needle carrier to the sample transport unit Check the sample transport connectors on the main board sample transport Check the connector on the bottom of the sample transport Change the needle and the seat Change the needle carrier assembly G1367 60010 Change the sample transport unit Change the main board G1367 69520 1260 Infinity Autosampler User Manual Troubleshooting and Diagnostics 6 8 Sample Location Error Probable cause Suggested actions 1 Check the plate configuration in the user interface Ensure the right vials and plates are use
72. er magnet is oriented with the correct pole toward the magnet sensor on the main board and positioned in the side cover such that it is close enough to the magnet sensor Replace the main board G1367 69520 1260 Infinity Autosampler User Manual Troubleshooting and Diagnostics 6 Initialization Fails The initialization fails to complete its required movements resulting in one or more of a variety of possible error messages The error message produced depends on when the failure occurred during the initialization Gather the revisions information user interface error and logbook information and error code information as described on page 1 Also consider anything which may have been done just before the initialization failure occurred To date we have seen initialization errors occur for a variety of reasons As an overall troubleshooting plan follow the suggested actions described bellow If this does not help take the information from the instrument logbook refer to the appropriate section in the next chapter and follow the recommended actions step by step Probable cause Suggested actions 1 Turn the sampler off manually change the X position of the sample transport and rotate the needle carrier to a different position turn on the sampler again If there is any weight on top of the sampler remove that weight turn off the sampler and then turn it on again 1260 Infinity Autosampler User Manual 121 6 T
73. esive c