- MPW MED. INSTRUMENTS Spółdzielnia Pracy
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1. centrifuge Cat No 10223 e A i W 20223e ENG user manual Cat No MED INSTRUMENTS 2012 11 26 USER MANUAL LABORATORY CENTRIFUGE MPW 223e A Read this before use mpw mpw pl www mpw pl MPW MED INSTRUMENTS 46 Boremlowska Street 04 347 Warsaw Poland tel 48 22 610 81 07 service fax 48 22 610 55 36 IVD Contents Application ZA 2s 2N 2 2 3 1 a a 3 4 Technical data Accessories Basic accessories Optional accessories Exploitation materials Installation Unpacking of the centrifuge Location Connection to mains Fuses Description of the centrifuge 4 1 General description Safe working conditions ae 2 S3 5 4 I5 Operating personnel Guarantee period and operation life Safekeeping period Hints on centrifuging Hazards and precautions Operation of the centrifuge 6 1 6 2 6 3 6 4 6 5 6 6 6 7 6 7 1 6 7 2 6 7 3 Service elements Mounting of the rotor and accessories Construction and safety measures Drive Data setting and read out Controls Safety devices Cover lock Rest state check Unbalanced load checking system 7 Description of the centrifuge operating elements 7 1 Control panel 7 2 Switching the centrifuge on 7 2 1 Selection of the program 7 2 2 Start of the program 7 2 3 Emergency stop 7 2 4 End of the centrifuging 7 2 5 Programming 7 3 Mathematical rel2. all test tubes are filled with the fluid with 1 2 g cm density If density of the centrifugated liquid is higher than 1 2 g cm then test tubes could be filled only partially or one shall limit operation speed of the centrifuge that is being calculated from the formula 1 2 Nperm n max X vr E 4 y specific gravity cm Nmax maximum rotational speed RPM Nperm permitted speed 8 Cleaning disinfection maintenance CAUTION Use safety gloves for operations specified below 8 1 Cleaning of the centrifuge Prior to start of cleaning and disinfection of the centrifuge one shall put the safety gloves on For cleaning shall be used water with soap or other water soluble mild detergent One should avoid corrosion inducing substances and aggressive substances It is prohibited to use alkaline solutions inflammable solvents or agents containing abrasive particles 8 2 Cleaning of the accessories In order to ensure safety operation one shall in regular way carry out periodical maintenance of the accessories Manufactured rotors buckets and round carriers have to withstand steady high stresses originated from the field of gravitation Chemical reactions as well as corrosion combination of variable pressure and chemical reactions can cause corrosion or destruction of metals Hard to observe surface cracks increase gradually and weaken material without visible symptoms In the case of observation of surface damage crevi
3. homogeneous density equal to 1 2 g cm or smaller when centrifugation is carried out at maximum speed When fluids of higher density shall be used then it is necessary to limit the speed see point 7 3 3 Maximum load One must not to use the rotors buckets and round carriers with symptoms of corrosion or other mechanical defects One must not subject to centrifugation substances of high corrosion aggressiveness which could cause material impairment and lower mechanical properties of rotors buckets and round carriers One must not use rotors and accessories not admitted by the manufacturer It is permitted however to use commercial glass and plastic test tubes intended by manufacturer of these test tubes for centrifugation in laboratory centrifuges It is distinct warning against using accessories not specified in the User manual Cracking of test tubes not intended for centrifugation can result in dangerous unbalance One must not carry out centrifugation with the rotors with taken off or not tight driven caps One must not lift or shift the centrifuge during operation and rest on it One must not stay in the safety zone within 30 cm distance around the centrifuge neither leave any things e g glass vessels within this zone It is prohibited to put any things on the centrifuge 10 6 Operation of the centrifuge 6 1 Service elements Cover of the centrifuge Viewing port Emergency cover release Key for emerge
4. least 30 cm Ensure sufficient ventilation Fix firmly the rotor on the motor shaft Avoid unbalance Load opposite buckets with the same accessories Centrifugation of the test tubes of different dimensions In principle it is possible to centrifuge test tubes of different dimensions however it is absolutely necessary in such cases that opposite round carriers have to be the same The test tubes shall be not only inserted symmetrically but round carriers and their hangers shall be equally loaded It is not allowed to operate centrifuge with asymmetric loads applied to rotors and buckets 9 Load all places in rotors 10 Fill test tubes outside the centrifuge 11 Glass tubes shall be test tubes intended for centrifuges of proper strength enabling centrifugation with acceleration up to 5000 x g 12 Fill in the test tubes with the medium of the same weight in order to protect the centrifuge against unbalance 13 Lubricate the rotor journal pins 14 Use only accessories kept in good condition 15 Protect equipment against corrosion using accurate preventive maintenance 16 Infectious materials could be processed in closed buckets only 17 It is prohibited to centrifuge explosive and inflammable materials 18 It is prohibited to centrifuge substances prone to reacting in result of supplying high energy during centrifugation ie ee ee 5 5 Hazards and precautions 1 10 11 12 13 14 15 16
5. the centrifuge shall not be located near the radiators and shall not be subjected to direct sunlight The table for the centrifuge shall be stable and shall have flat levelled table top Safety zone has to be established around the centrifuge with the minimum radius of 30 cm Under normal operating conditions ambient temperature shall not drop below 15 C or raise above 35 C In the case of changing location from a cold to warm one there will occur condensation of water inside the centrifuge It is important then that sufficient time shall be allowed for drying the centrifuge prior to restarting of the centrifuge minimum 4 hours 3 3 Connection to mains Supply voltage given on the rating plate has to be consistent with local supply voltage MPW MED INSTRUMENTS laboratory centrifuges are I safety class devices and are provided with the three core cable of 2 5 3 2 m length with the plug resistant to dynamic loadings Mains socket shall be provided with the safety pin Neutral grounding of mains socket safety pin has to be verified by authorized services This verification has to be carried out each time when mains socket is being replaced It is recommended to install emergency cut out that shall be installed far from the centrifuge near the exit door from the room or outside the room Supply voltage 230 V 50 60 Hz optionally 115 V 50 60 Hz 3 4 Fuses The centrifuge has standard protection with the WTA T 4A 250 V fuse situated in the pl
6. the fact that the centrifuge parts important because of safety reasons are not damaged This remark is specifically important for 1 Motor suspension 2 Motor shaft concentricity 3 Fixing the pins in the bucket 4 Centrifuge accessories and especially structural changes corrosion preliminary cracks abrasion of metal parts 5 Screw joints 6 Inspection of the rotor assembly 7 Inspection of bioseals of the rotors if such are used 20 11 Conditions of repairs Manufacturer grants to the Buyer a guarantee on conditions specified in the Guarantee Certificate Buyer forfeits the right to guarantee repair when using the device inconsistently with the Operating Instruction provisions when damage resulted from the User s fault Repairs should be carried out in authorized service workshops granted with the MPW Certificate The centrifuge shall be sent to repair after decontaminating disinfection Information about authorized service workshops could be obtained from the Manufacturer 1 e 12 Manufacturer s data MPW MED INSTRUMENTS SPOLDZIELNIA PRACY 46 Boremlowska Street 04 347 Warsaw Poland tel 48 22 610 56 67 service 48 22 610 81 07 fax 48 22 610 55 36 internet http www mpw pl e mail mpw mpw pl 13 Information about Distributor DISTRIBUTOR 21 MED INSTRUMENTS Y DECLARATION OF CONFORMITY Product Laboratory centrifuge Model MPW 223e Product classification on the basis o
7. the rotor chamber it is recommended to place on the bowl strip of vaseline from the top down to bottom Then rotor shall operate for several minutes at moderate speed Glass and metal particles will collect on lubricated area and could be easily removed with the piece of cloth together with the grease This operation can be repeated in case of need 9 Emergency conditions service 9 1 Troubleshooting Majority of faults could be cancelled by switching the centrifuge OFF and then ON After switching the centrifuge ON shall be displayed parameters of the recently implemented program and buzzer signals consisting of four successive tones In the case of short duration power failure the centrifuge terminates cycle Please find below the most frequent faults and their repair metods display active and no check buzzer sound 2 Centrifuge does not start START key pushing does not generate reaction or single tone only Rotor stopping symbol S is not displayed yet Wait till rotor stops and the rotor stopping symbol is displayed Cover opening symbol O is displayed Close cover S symbol that means stop should be displayed LED STATUS diode is blinking Centrifugation cycle in progress push STOP key or wait till cycle ends Indications show a cycle in progress but the Switch power supply OFF ON If fault still persists then motor does not start call service 19 3 Programming function not active It is impossible to r
8. 17 18 Prior to starting the trial of switching the centrifuge on one shall read exactly all sections of this instruction in order to ensure smooth run of operation avoiding damages of this device or its accessories Centrifuge can be operated by laboratory personnel getting acquainted with the Instruction Manual Centrifuge must not be transported with the rotor mounted on the motor shaft One must use original rotors test tubes and spare parts only In case of faulty operation of the centrifuge one shall ask for assistance of service of the MPW MED INSTRUMENTS Company or its accredited representatives It is prohibited to switch the centrifuge on if it is not installed properly or rotor is not fitted correctly The centrifuge must not be operated in places where explosion hazard appears as it is not of explosion proof make It is prohibited to centrifugate materials which could generate inflammable or explosive mixtures when in contact with air It is prohibited to subject to centrifugation toxic or infectious materials without taking proper safety measures work in properly adapted rooms personal safety equipment Proper disinfection procedures have to be carried out when dangerous substances contaminated the centrifuge or its accessories One must not open the cover manually in emergency procedure when rotor is still turning One must not exceed limit load set by the manufacturer Rotors are intended for fluids of average
9. 95 13170 13233 13245 13246 13247 13249C 13250 13265 13266 13267C 13276 13329 14024 14035 14036 14043 14071 14089 14082 14088 14169 14181 14186 14187 14188 Type of rotor Angle Rotor capacity Max rpm Rmax cm Angle rotor 30 12x15 ml 4000 11 9 Angle rotor 45 4x30 25 ml 4000 11 9 Angle rotor 30 10x30 25 ml 4000 12 Angle rotor 30 12x30 25 ml 4000 9 6 Angle rotor 30 10x50 ml 4000 11 7 Angle rotor 30 24x15 10 ml 4000 11 7 Angle rotor 45 8x15 ml 4000 11 5 Angle rotor 30 30x15 10 ml 4000 13 3 Swing out rotor 4x100 ml 4000 13 8 Swing out rotor 8x15 10 ml 4000 14 Swing out rotor 4x7x10 5 ml 4000 12 9 Swing out rotor 4x50 ml 4000 13 8 Bucket for test tubes 15 10 7 6 ml 013 17x100 120 mm for rotor No 11237 11486 11487 11502 12193 Bucket for test tubes 10 6 5 ml 013 17x70 85 mm for rotor No 11486 11487 11237 11502 12193 Bucket 100 ml without thread for rotor 12183 for carriers 14186 14190 14024 14181 14192 14224 Bucket 100 ml with cap for round carrier No 14186 14190 14024 14181 14192 Hanger 2x15 ml with 2 buckets Falcon No 13080 for rotors No 12183 Bucket 12x5 ml for test tubes p 13x81 mm for rotor 12232 Bucket for rotor No 12232 for round carriers No 14169 14235 14238 14239 14240 14242 Bucket for test tubes 5x5 ml 12x75 85 mm for rotor No 12485 Bucket 4x10 ml for rotor No 12485 Bucket 2x15 ml for rotor No 12485 Bucket 50 ml for test tube
10. ations 7 3 1 RCF relative centripetal force 7 3 2 Nomogram of relationship rotational speed centrifuging radius RCF 7 3 3 Maximum load 8 Cleaning disinfection maintenance 8 1 Cleaning of the centrifuge 8 2 Cleaning of the accessories 8 3 Sterilization and disinfection of the rotating chamber and accessories 8 4 Lubrication 8 5 Glass cracking 9 Emergency conditions service 9 1 Troubleshooting 10 Safety work 10 1 Safety work inspection procedures 10 2 Inspection procedures carried out by the operator 11 Conditions of repairs 12 Manufacturer s data 13 Information about Distributor Annexes Statement of conformity Declaration of decontamination repair Declaration of decontamination return 1 Application The MPW 223e centrifuge is a table top laboratory centrifuge intended for in vitro diagnostic IVD Its construction ensures easy operation safe work and wide range of applications in laboratories engaged in routine medical analyses biochemical research works etc It is intended for separation of mixtures suspensions and systemic fluids into constituents of different densities under influence of the centrifugal force This centrifuge is not biotight and therefore during centrifugation of preparations that require biotightness one has to use closed and sealed buckets and rotors It 1s prohibited to centrifuge caustic inflammable and explosive preparations in the cen
11. ce or other change as well the corrosion given part rotor bucket etc shall be immediately replaced In order to prevent corrosion one has to clean regularly the rotor together with the fastening bolt buckets and round carriers Cleaning of the accessories shall be carried out outside of the centrifuge once every week or still better after each use Then those parts shall be dried using soft fabric or in the chamber drier at ca 50 C Especially prone to the corrosion are parts made of aluminium For cleaning them one should use very neutral agent of pH value within 6 8 range It is forbidden to use alkaline agents of pH above 8 In this way substantially is increased useful service life and diminished susceptibility to corrosion Accurate maintenance also increases service life and protects against premature rotor failures Corrosion and damages resulting from insufficient maintenance could not be object of claims lodged against the manufacturer 8 3 Sterilization and disinfection of the rotating chamber and accessories All standard disinfecting agents can be used The centrifuge and the equipment are made of different materials which should be taken into account Consider the temperature resistance of different materials when using steam for sterilisation It should be mentioned that air tight containers should be used for centrifuging for example infectious materials in order to prevent them from penetrating the centrifuge 18 Conta
12. d operations facilitates to operator programming of condition of the centrifuge Operation of the centrifuge is simple and self evident 6 5 Controls Microprocessor control system being used in the centrifuge ensures the following possibilities of setting of parameters of operation stepwise rotational speed selection within the range from 300 up to 4000 rpm every 100 rpm setting of the centrifugation time within the range from 1 up to 99 minutes selection of SHORT short duration operation setting for pre programmed speed 12 6 6 Safety devices Apart from the above described passive devices and safety measures there exist as well active devices and elements as follows 6 6 1 Cover lock The centrifuge can be started only with properly closed cover The cover can be opened only after stopping the rotor In the case of emergency opening of the cover during operation the centrifuge will be immediately switched off and the rotor will be braked until stopping completely With opened cover the drive is completely disconnected from power which makes it impossible to start the centrifuge Emergency cover release In case of e g power failure there still exists possibility of manual opening of the cover At right side of the casing there is situated small blanked hole where one shall put key cat no 17162 or bar 2 mm and push it and cover opens by itself CAUTION Cover can be released and opened provided that rotor wil
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14. e during centrifuging it is possible to interrupt the process and stop the rotor quickly with single pushing of STOP key 7 2 4 End of the centrifuging After termination of time of centrifugation preset in the program braking follows At the end of deceleration the rotational speed drops at slower rate in order to ensure soft settling of rotor carriers Stopping is followed by buzzer signal and is displayed by S letter symbol After pushing COVER pushbutton the cover opens and O symbol is displayed 7 2 5 Programming Programming mode is activated with pushing SPEED and TIME keys after selection of parameters of the program which one would like to save or change Acceptance of preset parameters is done by pushing STOP key One can save one program only 15 7 3 Mathematical relations 7 3 1 RCF relative centripetal force RCF acceleration is the acceleration generated by the rotor rotary motion acting upon tested product and it can be calculated according to the formula RCF 11 18 r n 1000 where RCF x g r cm n rpm Depending on the distance of particles of the tested product from the axis of rotation one can find from above formula minimum RCF average RCF or maximum RCF On the basis of preset RCF value and given radius of the bottom in the bucket one can calculate from the formula the rotational speed to be set in the program of centrifuging Selection of the time of sedimentation and the RCF val
15. ecord parameter values Call service to memory last recorded program can not be recalled Disturbances on displays possible too 4 Centrifuge starts but does not accelerate overload closing the cover 5 Centrifuge starts and accelerate then decelerate and stops Unbalanced load symbol U is displayed Check Offence of acceptable unbalance value of the Rotor screw fixing rotor of test tubes Symetrical load rotor with test tubes Equalization of vessels loads Rotor stopping S symbol not displayed yet Rotor is still rotating Wait for stopping of the rotor and after pushing cover opening key single tone displaying of the S symbol is audible Nothing is displayed Check the centrifuge power supply Rotor stopping S symbol is displayed but Call service cover cannot be opened 10 Safety work 10 1 Safety work inspection procedures From the point of view of operational safety the centrifuge has to be subjected to inspection carried out by authorized service engineer or especially trained experts at least once every year in the state of operational readiness The reason for more frequent inspection could be for instance more frequent unbalance cases or corrosion inducing environment Results of inspections repairs and tests have to be recorded and kept on file Operating Instruction shall be stored in the centrifuge use place 10 2 Inspection procedures carried out by the operator Operator has to pay special attention to
16. er to avoid overflows 11 CAUTION Centrifuge will tolerate small weight differences occuring during loading of rotors However it is recommended to equalize vessels loads as much as possible in order to ensure minimal vibrations during operation 12 Maximum unbalance amounts up to 15 g 13 In order to prolong lifetime of the rotor and gaskets rotors shall be lubricated with the maintenance oil while gaskets and threaded parts shall be lubricated with Vaseline cat no 17201 basic accessories 14 For replacement of the rotor one shall release clamping by several turns of the bolt and then using both hands grab the rotor at opposite sides taking it away from drive shaft by pulling it up 6 2 Construction and safety measures The centrifuge has rigid self supporting structure Housing is made of plastic while front is made of steel sheet Cover is fixed on steel axles of hinges and from the front is locked with electromagnetic lock blocking possible opening during centrifugation Bowl forming the rotation chamber is made of acid resistant steel sheet 6 3 Drive Drive constitutes brushless induction motor of low noise level free of carbon brushes This solution eliminated the danger of contamination the preparations with carbon dust 6 4 Data setting and read out Data setting and read out system forms hermetically closed keyboard with distinctly accessible operation points Easily readable display signalling individual performe
17. est tubes 15116 for bucket 13182 13184C 14196 Round carrier for bucket No 13184 for bucket No 13182 13184C 14235 Round carrier 12x5 ml 12x75 mm for bucket No 13233 and rotor No 12232 14238 Round carrier for test tube 15121 for bucket 13233 14239 Round carrier for test tube 13 5mm x 75 mm d for bucket 13233 14240 Round carrier for test tubes 15011 15015 15128 for bucket 13233 14242 Round carrier for test tube 15016 for bucket 13233 14248 Round carrier for test tubes 30 25 ml 29 8 26 mm for bucket No 13266 13267C 13276 13250 13249C 14255 Round carrier 7 ml p 25 13 mm for bucket No 13329 14256 Round carrier 15 10 ml 25 17 mm for bucket No 13329 15040 Polypropylene test tube 100 ml with stopper 44 5x103 mm 15046 Polypropylene test tube 14 ml with stopper 16 8x105 mm 15048 Polypropylene test tube 15 ml Nalgene 16 x113 mm 15050 Polypropylene test tube 15 ml Falcon 17 21x120 mm for bucket No 13080 15052 Polypropylene test tube with conical bottom 50 ml 030 35x120 mm for bucket No 13249 and 13276 15053 Polypropylene test tube 10 ml with stopper 016 19x100 mm for bucket No 13080 15054 Polypropylene test tube 6 ml with cap p 11 5x92 mm 15055 Polypropylene test tube 30 ml with cap 24 8x100 mm for bucket No 13249 with reduction sleeve 14248 and for bucket No 13329 15117 Glass tube 25 ml 25x100 mm for bucket No 13249 with reduction sleeve No 14248 and for bucke
18. f Non classified A or Band not for the Directive 98 7 self testing PN EN ISO 13485 2012 AC 2013 03 PN EN 61010 2 PN EN 13612 2 PN EN 61326 2 6 2 e standard PN EN ISO 15223 1 2012 m O peezes zapu MPW MED INSTRUMENTS ni ar d SP DZIELNIA PRACY Wojciel Tiii hera s Warsaw 46 Boremlowska Street mgr a se Quality policy in line with ISO 9001 2008 Certifying authority MPW MED INSTRUMENTS SP DZIELNIA PRACY w Warszawie Directive 2004 108 WE EMC Warsaw 13 11 2014 DECLARATION OF DECONTAMINATION In order to protect our employees please fill out the declaration of decontamination completely before sending centrifuge to the manufacturer repair 1 Device SAVDO qj q j OAT A TO E A NA LEANNE O Aa a ee EE EOE TE ona 2 Description of decontamination see user Manual 3 Decontamination carried out by SAAME qj RONNE E aE te p E EEEE EE aa e r Oean aai Eoia 4 Date and signature gt lt aisdsscuscrceronsasts ovsacysagactecascddesosbusaeasetoderavegersasvaaeaeonatens DECLARATION OF DECONTAMINATION In order to protect our employees please fill out the declaration of decontamination completely before sending centrifuge to the manufacturer return 5 Device STYPE qj qj j _ _ _essineirnaibrena A AART serial No 6 Description of decontamination see user Manual 7 Decontamination carried out by name 8 Datean
19. iners and rotors can be autoclaved at 121 124 C for 15 minutes at 215 kPa The centrifuge shall be disinfected with disinfecting agents used normally in the health care sector for examples look at amp 2 2 The user is responsible for disinfecting the centrifuge properly if a dangerous material has been spilled outside or inside the unit Always wear protective gloves when performing the work described above 8 4 Lubrication The rotor pins shall be always lubricated with the Vaseline cat no 17201 basic accessories In this way is ensured uniform deflection of the buckets and quiet centrifuge operation 8 5 Glass cracking In the case of glass cracking one shall wear safety gloves and all debris shall be accurately removed Rubber inserts shall be exactly cleaned or possibly replaced Otherwise one has to take into account the following possibilities Glass particles left in the rubber cushion pad will cause once more glass cracking Glass particles left in containers make impossible uniform deflecting of the buckets and round carriers resulting in unbalance Glass particles left in the rotor chamber cause metal abrasion because of strong air circulation This dust will not only contaminate the centrifuge chamber rotor buckets carriers and centrifuged material but will cause as well damages of surfaces of the accessories rotors and the centrifuge chamber For complete removal of glass particles and metal dust from
20. l be in rest state 6 6 2 Rest state check Opening of the centrifuge cover is possible only with the rotor in the state of rest This state is being checked by the microprocessor which recognizes and signals the rest state prior to opening the cover with letter S Stop 6 6 3 Unbalanced load checking system The centrifuge is equipped in unbalance sensor for protection Drive is switched off during acceleration or operation of the centrifuge when loads of opposite buckets or carriers in rotor are unbalanced and message U will be displayed CAUTION See paragraph 9 1 subparagraph 5 13 7 Description of the centrifuge operating elements Power switching ON OFF is carried out with master switch situated on back of the centrifuge All settings on the centrifuge are done by means of the control panel Panel comprises control pushbuttons displays and signalling LEDS 7 1 Control panel MPW MED INSTRUMENTS Centrifuge MEW ZZZe START STOP Control panel placed on front casing wall serves for controlling centrifuge operation Control panel comprises the following elements 1 Upper display field S 4 digits rpm RCF 4 digits x g 2 Lower display field T 4 digits m s S STOP O opened cover U unbalance 3 Error signalling 3 Rotor status signalling STATUS COVER blinking rotor rotates not illuminated rotor does not rotate 4 Function key START 5 Function key STOP 6 Function key COVER
21. n a gt Buzzer serves for signalling function recording and determination of the centrifuge status gt START key serves for starting centrifugation program with parameters presented on display gt STOP key serves for interrupting centrifugation program in any program phase and braking the rotor saving of preset SPEED and TIME centrifugation parameters gt AA COVER key serves for opening of the cover SPEE gt i SPEED keys serve for speed programming gt TIME keys serve for time programming gt 3 key serves for short duration operation 14 7 2 Switching the centrifuge on After switching power ON the control system calls recently implemented program and displays in relevant fields rotational speed duration of centrifugation and cover opening status Provided that rotor in the centrifuge is stopped it is possible to open the cover by means of COVER key Stopped rotor status is displayed as a S letter symbol in the display field When this symbol is not already displayed then one must wait till this rotor stops and the above mentioned symbol appears 7 2 1 Selection of the program Control panel can save 1 program preset by the user Program acceptance consists in pushing STOP key 7 2 2 Start of the program After acceptance of the program and checking if rotor was mounted centrifuge can be started with pushing START key provided that cover is closed 7 2 3 Emergency stop At any tim
22. ncy lid opening Control panel Clamping bolt Rotor Motor shaft Drawing No 1 General view 6 1 Mounting of the rotor and accessories L Connect the centrifuge to mains master switch at back wall of the centrifuge Open the cover of the centrifuge pushing the pushbutton COVER Prior to putting the rotor in one has to check 1f rotating chamber is free of impurities e g such as dust glass splinters residues of fluids that must be taken away J One shall release with special spanner clamp on the motor shaft and fit the rotor on the motor shaft driving it home on the cone 4 Screw in the bolt for fixing the rotor clockwise and screw it tightly home with the supplied 11 spanner 3 Swing out rotor have to be provided with the buckets in all seats One should to remember that every buckets swings individually Bucket suspension studs should be lubricated periodically with vaseline 6 In the case of rotors designed with the cover they must not be used without it Rotor covers must be closed exactly Rotor covers ensure smaller drags of the rotors proper setting of the test tubes and airtight sealing ve One should use only buckets intended for selected types of the rotor see p 2 1 Accessories 8 Fill test tubes outside the centrifuge 9 Put on or screw the caps on vessels and rotors when they have such 10 In the case of centrifuging in angle rotor test tubes buckets have to be filled properly in ord
23. s 50 30 25 ml with thread 30x99 mm for rotor No 12485 Bucket 50 ml for test tubes 50 30 25 ml without thread b 30x96 mm for rotor No 12485 Bucket for Arthrex ACP system for rotor 12183 Bucket 50 ml for rotor 14089 14248 Bucket 50 ml for rotor 14089 14248 Bucket 1x50 30 25 ml without thread 030x96 mm for rotor No 11333 Bucket 30 25 ml 025x100 mm for rotor No 11329 11332 and 11257 Round carrier for test tubes 15050 for buckets 13182 13184C Round carrier for test tubes 15046 for bucket 13276 Round carrier for test tubes 13 5mm x 92 mm for bucket 13276 Round carrier for test tubes 15419 for bucket 13276 Round carrier for test tubes 15055 15117 for bucket 13276 Round carrier for test tubes 15050 for buckets 13250 13276 13266 13267C Round carrier for test tubes 7 5 ml 017 6 13 3 mm for buckets No 13080 and 13081 Round carrier for test tubes 7 5 ml 017 3 13 mm for bucket No 13080 Round carrier for test tubes 8x5 ml p 13x81 mm for bucket 13233 and rotor 12232 Round carrier for test tubes 15119 for bucket 13182 13184C Round carrier 4x7 ml b 13 1x100 mm for bucket No 13184 Round carrier 4x10 ml 16 1x100 mm for bucket No 13182 13184C Rubber cushion for test tubes 100 50 30 25 ml for bucket No 13182 13184C 14189C Round carrier 50 ml Falcon p 44 5 30 mm for bucket No 13182 13184C 14190C Round carrier 30 25 ml b 44 5x25 5 mm for bucket No 13182 13184C 14192C Round carrier for t
24. t No 13329 15118 Glass tube 10 ml 16x100 mm for bucket No 13080 15119 Glass tube 7 ml 12x100 mm for carrier No 14082 in bucket No 13080 15120 Glass tube 5 ml 12x75 mm for carriers No 14082 14235 in bucket No 13081 15121 Polypropylene test tube 10 ml with stopper 17x70 mm for bucket No 13081 and carrier No 14234 15419 Polypropylene test tube 5 ml 912x75 mm for carrier No 14082 in bucket No 13081 _ while stocks last 2 2 Exploitation materials Only proprietary elements listed in the equipment list shall be used in the centrifuge as well as centrifuge test tubes with suitable diameter length and strength The use of test tubes made by other companies shall be consulted with the manufacturer of the centrifuge The centrifuge shall be cleaned and disinfected with agents used normally in the health care sector such as Aerodesina 2000 Lysoformin 3000 Malseptol Malsept SF Sanepidex Cutasept F 3 Installation 3 1 Unpacking of the centrifuge Open the package Take out the cardboard box containing the accessories Take out the centrifuge from the package Keep the package and packing materials at hand for possible transport at a later date 3 2 Location Almost all energy being supplied to the centrifuge is transformed into heat and then emitted to the environment This is the reason why proper ventilation is essential Ventilation ducts situated in the centrifuge have to be fully efficient Moreover
25. trifuge 2 Technical data Manufacturer MPW MED INSTRUMENTS SPOLDZIELNIA PRACY 46 Boremlowska Street Warsaw Poland Type MPW 223e Mains L1 N PE V Hz 10 230 V 50 60 Hz or 115 V 50 60 Hz Maximum power consumption 120 W Interference level PN EN 55011 Noise level 50 dB Rotational speed range 300 4000 rpm Maximum capacity 500 ml Maximum acceleration RCF 2504 x g Maximum kinetic energy 1536 Nm Time range 1 99 min SHORT short duration operation Physical data Depth 435 mm Width 355 mm Height 270 mm Weight 13 kg Centrifuge operation conditions Environmental temperature Relative humidity at ambient temperature Installation category Degree of pollution Protection zone 2 1 Accessories PN EN 61010 1 p 1 4 1 2 440 C lt 80 II PN EN 61010 1 2 PN EN 61010 1 300 mm 2 1 1 Basic accessories enclosed with every centrifuge 17099T spanner for the rotor pes 1 17201 vaseline 20ml pes 1 17142 complete clamp pes 1 17162 key for emergency lid opening pes 1 17861 fuses WTA T 4 A 250 V pes 2 17866 power cord 230 V pes 1 17867 power cord 115 V optionally pes 1 20223e ENG Operating Instruction pes 1 2 1 2 Optional accessories Depending on customer s need MPW 223e centrifuge can be provided with accessories specified below Cat No 11237 11257 11329 11332 11333 11486 11487 11502 12183 12193 12232 12485 13080 13081 13182 13184c 131
26. ue shall be carried out experimentally for a given product Once every 100 rpm electronic circuit automatically calculates and displays averaged RCF value 16 7 3 2 Nomogram of relationship rotational speed centrifuging radius RCF Centrifuging radius cm 50 45 40 35 30 29 28 21 26 25 24 23 22 21 20 19 18 A 17 16 14 13 12 11 10 Formula used for calculation of this nomogram 2 R C F 11 18 r n 1000 where R C F multiple of gravitational speed r centrifuging radius cm n rotational speed r p m g gravitational acceleration Example of making use of the nomogram A 14 4 cm B 4600 r p m C 3400 x g RCF n 1000 _ 11 18 r RCF 2 0 ni T000 r 17 R C F x g multiple of gravitational acceleration 100000 50000 40000 30000 20000 15000 10000 8000 6000 5000 4000 3000 2400 2000 1500 1200 1000 800 600 500 400 300 200 150 120 100 80 60 50 40 30 25 20 O oOo AO r p m 20000 15000 14000 13000 12000 11000 10000 9000 8000 7000 6000 5000 4500 4000 3500 3000 2500 2000 1500 1400 1300 1200 1100 1000 900 800 700 600 500 400 300 200 7 3 3 Maximum load In order to avoid overloading of the rotor one shall observe maximum load which is recorded on every rotor Maximum permissible load is reached when
27. ug in socket and master switch unit at back wall of the centrifuge 4 Description of the centrifuge 4 1 General description New generation of MPW MED INSTRUMENTS laboratory centrifuges is provided with modern microprocessor control systems very durable and quiet asynchronous brushless motors and accessories consistent with modern requirements 5 Safe working conditions 5 1 Operating personnel MPW 223e Laboratory Centrifuge can be operated by laboratory personnel after getting acquainted with the Instruction Manual User manual shall be always near the centrifuge User manual must be constantly at hand 5 2 Guarantee period and operation life Guarantee period for MPW 223e centrifuge amounts to minimum 24 months Principles are specified in guarantee certificate The service life of the centrifuge specified by the manufacturer amounts to 10 years After termination of guarantee period it is necessary to carry out annual technical inspections of the centrifuge made by authorized service of manufacturer The manufacturer reserves the right to make modifications at produced goods 5 3 Safekeeping period Maximum period of storage of not used centrifuge amounts to year After this period one should ask authorized service to carry out an inspection of the device 5 4 Hints on centrifuging Set the centrifuge in horizontal position on rigid base Ensure safe location Ensure free space around the centrifuge within at
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