Hoefer SQ33 Sequencer
Contents
1. Gebruik het instrument niet in extreem hoge luchtvochtigheid boven 80 Condensatie voorkomen door te laten de eenheid evenwicht tot omgevingstemper atuur bij het nemen van het instrument van een kouder naar een warmere omgeving Om voldoende koeling mogelijk te maken ervoor te zorgen dat de ventilatieopeningen van het instrument niet worden gedekt piii T rkeit tietoja Finnish SQ33 Jos laitetta k ytet n tavalla ei m ritelty Hoefer Inc antama suojaus saattaa heiket T m v line on suunniteltu sis tiloissa laboratorion k yt t n Vain lis tarvikkeet ja osat hyv ksytty tai toimittamia Hoefer Inc voi k ytt k yt st yll pidosta ja huoltoa tuote K yt vain virtal hdett joka on CE merkitty ja turvallisuutta sertifioitu kansallisesti tunnustettu testauslaboratorio Turvallisuus Kannen on oltava paikoillaan ennen kuin kytket virtal hteen johtaa virtal hteeseen K nn kaikki virtal hteen ohjaa virta ja irrota virtajohdot ennen kuin irrotat turvallisuutta kansi Kierr t vain vett tai 50 50 vesi etyleeniglykoli l mm n vaihtimen l pi jos varusteena l kytke l mm nvaihtimen vesihanaan tai j hdytysnestett l hde josta vesi paine on s ntelem t nt Koskaan k ytt n pakkasnestett tai mik tahansa orgaan inen liuotin mihin tahansa osaan v line Orgaaniset liuot timet aiheuttaa korjaamatonta vahinkoa laitetta l k yt puskuria2. manual Hoefer SQ33 Sequencer Includes Fan Sensor Kit um SQ33 IM Rev B0 03 13 Ayo e f e r Page finder important Information aaa sa otra aule L Waste Electrical and Electronic Equipment WEEE sees eee ee eee IX 1 5033 Function and Description 1 ee e 2 3 SQ33 Operating Instructions ir 4 4 Installation and Operation of the Fan Sensor Ki li 5 5033 Care and Maintenance 111111111 13 6 Customer Service Information 11111111 14 Je DISPOSOT EE 15 Sa Ee ii aR O SEEGER blka 16 9 Ordering Totormaton 280 RR 18 Important Information English 5033 If this equipment is used in a manner not specified by Hoefer Inc the protection provided by the equipment may be impaired This instrument is designed for indoor laboratory use only Only accessories and parts approved or supplied by Hoefer Inc may be used for operating maintaining and servicing this product Only use a power supply that is CE marked or safety certified by a nationally recognized testing laboratory The safety lid must be in place before connecting the power supply leads to a power supply Turn all power supply controls off and disconnect the power leads before removing the safety lid Circulate only water or 50 50 water ethylene glycol through the heat exchanger if so equipped Do not connect the heat exchanger to a water tap or any coolant source w
3. 2 1 5 kg 38 x 120 x 120 mm 2 700 rpm gt 84 CFM 36 dBA 2 2 Environmental Conditions e This apparatus is intended for indoor use only e This apparatus can be operated safely up to an alti tude of 2 000 m e The normal operating temperature range is between 4 C and 65 C e Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C e The apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664 POLLUTION DEGREE 2 states that Normally only non conductive pollution occurs Occasionally however a temporary conduc tivity caused by condensation must be expected 3 SQ33 Operating Instructions 3 1 Regent Preparation and Gel Volumes For reproducibility and uniform polyacrylamide cross linking we recommend deionizing degas sing and filtration of acrylamide gel solutions prior to use Acrylamide solutions should be stored in a cool dark environment such as a refrigerator and allowed to reach room tempera ture prior to pouring Avoid exposure to heat and sunlight Polymerization conditions should be adjusted to effect polymerization within about 5 10 minutes Test a small volume in a vial prior to pouring the gel As a rough guide 100 ml of degassed 6 acrylamide gel will set in about 5 minutes at room temperature when gently mixed with 450 ul of freshly prepared 10 w v Ammonium persulfate plus 200 pl TEMED The setting time increases to a
4. 2 pieces SQ33 SP Fan Sensor Kit 115 VAC SQ33 HS 115V Fan Sensor Kit 230 VAC SQ33 HS 230V e pls Hoefer Inc 84 October Hill Road Holliston MA 01746 Toll Free 1 800 227 4750 Phone 1 508 893 8999 Fax 1 508 893 0176 E mail support hoeferinc com Web www hoeferinc com Hoefer is a registered trademark of Hoefer Inc 2013 Hoefer Inc All rights reserved Printed in the USA Hoefer
5. cuidado deve ser exercido em sua opera o Este instrumento foi projetado de acordo com a EN61010 1 2001 padr o de seguran a el trica No entanto ele deve ser usado apenas por operadores devidamente treinados Leia todo o manual antes de usar o instrumento e usar apenas de acordo com as instru es O instrumento deve ser sempre usado com o fio terra do cabo de alimenta o corretamente aterrado na tomada e Use apenas fios danificados el trica e equipamentos espe c ficos para as tens es que voc ir usar Todos os equipa mentos conectados alta tens o deve estar de acordo com EN61010 1 2001 Mantenha o instrumento o mais seco e limpo poss vel Limpe com um pano macio e mido Deixe o instrumento secar completamente antes de usar N o utilizar o aparelho de extrema umidade acima de 80 Evitar a condensa o deixando a unidade de atingir a temperatura ambiente ao tirar o instrumento a partir de um mais frio para um ambiente mais quente Para permitir uma refrigera o suficiente garantir que as aberturas do instrumento n o s o cobertos Informaci n importante Spanish SQ33 e Si este equipo se utiliza de una manera no especificada por Hoefer Inc la protecci n proporcionada por el equipo puede verse afectada Este instrumento est disehado para uso en laboratorio en interiores S lo los accesorios y partes homologado o suministrado por Hoefer Inc puede ser utilizado
6. the plastic lugs and lift the lid vertically with your fingers e Before use clean and dry the apparatus with distilled water only e Before use and then on a monthly basis check the unit for any leaks at the bonded joints Place the unit on a sheet of dry tissue and then fill with distilled water only to the maximum fill line Any leakage will be seen on the tissue paper If any leakage is seen do not attempt to repair or use the apparatus but notify Hoefer technical support immediately After each use wash all components with a suit able water soluble disinfectant to reduce the risk of infection Ensure that the connectors are clean and dry before usage or storage The cooling plate upper surface cannot be cleaned with acetone Methanol and ethanol can be used 5 1 Cleaning and Storage e After use thoroughly rinse all components in deionized water and gently dry the gold electrode connectors with a soft tissue e Ensure that the connectors are clean and dry before usage or storage e Never use organic solvents 5 2 Fan Sensor Kit Care and Maintenance e The unit can be cleaned as required when the mains supply is isolated e Cleaning should be carried out with a cloth moistened with water or tissues impregnated with 70 iso propyl alcohol No other cleaning solutions should be used e There are no internal operator serviceable parts in this unit If the power should fail the unit must be returned to the
7. All utrustning r ansluten till h gsp nning skall ske i enlighet med EN61010 1 2001 H ll instrumentet s torr och ren som m jligt Torka regel bundet med en mjuk fuktig trasa L t instrumentet torka helt f re anv ndning Anv nd inte instrumentet i h g luftfuktighet ver 80 Undvik kondens genom att l ta enheten j mvikt till rums temperatur n r du tar instrumentet fr n en kallare till en varmare omgivning F r att m jligg ra tillr cklig kylning se till att ventilerna i instrumentet inte omfattas pviii English R French R German R EES Italian R EEE Spanish D Swedish R ss Waste Electrical and Electronic Equipment WEEE This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment Ce symbole indique que les d chets relatifs a l amp quipement lectrique et lectronique ne doivent pas tre jet s comme les ordures m nag res non tri es et doivent tre collect s s par ment Contactez un repr sentant agr du fabricant pour obtenir des informations sur la mise au rebut de votre quipement Dieses Symbol kennzeichnet elektrische und elektronische Ger te die nicht mit dem gew hnlichen unsortierten Hausm ll entsorg
8. para el funcionamiento mantenimiento y reparaci n de este producto Utilice solamente una fuente de alimentaci n que lleva la marca CE o certificado de seguridad por un laboratorio de pruebas reconocido nacionalmente La tapa de seguridad deben estar en su lugar antes de conectar la fuente de alimentaci n conduce a una fuente de alimentaci n Gire todas las fuentes de energia controla y desconecte los cables de alimentaci n antes de retirar la tapa de seguridad Circule s lo agua o 50 50 de agua glicol de etileno a trav s del intercambiador de calor si lo tiene No conectar el inter cambiador de calor a un grifo de agua o de cualquier fuente de refrigerante donde la presi n del agua es regulada Nunca introducir anticongelante o cualquier disolvente org nico en ninguna parte del instrumento Los solventes org nicos puede causar dahos irreparables en el aparato No trabajar con temperaturas por encima de los m ximos de amortiguamiento especificados especificaciones t cnicas El recalentamiento puede causar dahos irreparables en el aparato Fan Kit Sensor e jAdvertencia Debido a que este instrumento puede desarrollar suficiente voltaje y corriente para producir una descarga el ctrica mortal se debe tener cuidado en su funcionamiento Este instrumento est dise ado de acuerdo con la norma EN61010 1 2001 norma de seguridad el ctrica No obstante debe ser utilizado s lo por operadores debidamente capacit
9. with passive ventilation If the fan kit is used the power can be increased up to 75 Watts Ensure that with the buffer system employed the voltage does not exceed the maximum recommended voltage The resistance of the gel changes throughout electropho resis when run at constant power so the starting and finishing voltages will be different O In general the gel temperature should reach 50 60 C At temperatures above this range there is the risk of generating smeared bands or damaging the gel plates 3 5 Ending the Run O Isolate the power supply before removing the connectors Remove the safety lid by gripping the edges and push ing down with your thumbs on the pegs located on the top of the unit Disconnect the leads to the upper and lower buffer chambers Open the upper buffer tank drainage tube The buffer will then flow into the bottom buffer tank O Remove the lower buffer chamber and carefully discard the buffer to waste O Dismantle the gel and separate the plates with a strong thin broad blade o plo D Note Do not pry the glass plates apart at the ears Concentrated leverage in any one area may damage the plates O After removing the gel clean the plates thoroughly in neutral detergent and rinse with distilled water A sheet of clean foam rubber placed in the bottom of the sink serves as a useful support and minimizes the risk of plate damage O Rinse buffer Cha
10. Handbuch bevor Sie das Ger t und verwenden Sie nur nach den Anweisun gen Das Ger t muss immer mit dem Schutzleiter des Stromka bels korrekt mit der Erde an der Steckdose geerdet werden Verwenden Sie nur unbesch digte elektrische Dr hte und spezielle Ausr stungen f r die Spannungen die Sie verwen den 2001 Alle Ger te die an hohe Spannung sollte in bereinstimmung mit EN61010 1 sein Halten Sie das Ger t trocken und sauber wie m glich Wischen Sie regelm ig mit einem weichen feuchten Tuch Lassen Sie das Ger t vollst ndig trocknen bevor Gebrauch Betreiben Sie das Instrument nicht in extreme Luftfeuchtig keit ber 80 Kondensation vermeiden indem das Ger t auf Raumtemperatur quilibrieren wenn man das Ger t von einer kalten in eine w rmere Umgebung Um eine ausreichende K hlung zu erm glichen stellen Sie sicher dass die L ftungsschlitze des Ger tes nicht verdeckt sind Informazioni importanti Italian SQ33 e Se apparecchiatura viene utilizzata in un modo non specifi cato dal Hoefer Inc la protezione fornita dallo strumento DUO essere compromessa Questo strumento stato progettato per I uso in laboratorio interno Solo accessori e ricambi approvati o forniti da Hoefer Inc pu essere utilizzato per il funzionamento la gestione e la manutenzione di questo prodotto Utilizzare solo un alimentatore che la marcatura CE o di sicurezza certificato da un laboratorio di pro
11. ados Lea este manual en su totalidad antes de usar el instrumento y utilizar solamente de acuerdo con las instrucciones pvil El instrumento se debe utilizar siempre con la toma de tierra del cable de alimentaci n correctamente conectado a tierra en la caja de enchufe e Use s lo cables el ctricos intactos y equipo espec fico para las tensiones que va a utilizar Todos los equipos conectados a alta tension debe ser de acuerdo con la norma EN61010 1 2001 Mantenga el instrumento lo m s seca y limpia posible Limpie peri dicamente con un pa o suave y h medo Deje que el aparato se seque completamente antes de su uso e No utilice el instrumento en condiciones de extrema humedad superior al 80 Evite la condensaci n permi tiendo que la unidad alcance la temperatura ambiente en el momento de tomar el instrumento a una m s fr a que la temperatura normal e Para permitir la refrigeraci n adecuada aseg rese de que los orificios de ventilaci n del equipo no est n cubiertos Viktig information Swedish SQ33 Om denna utrustning anv nds p ett s tt som inte specifi ceras av Hoefer Inc skydd som utrustningen kan f rs mras Detta instrument r avsett f r inomhusbruk laboratorium bruk Endast tillbeh r och reservdelar godk nda eller levereras av Hoefer Inc kan anv ndas f r drift underh ll och service av denna produkt Anv nd endast en str mk lla som r CE m rkt eller s kerhet c
12. ation en laboratoire Vint rieur uniquement Seuls les accessoires et les pi ces approuv es ou fournies par Hoefer Inc peut tre utilis pour le fonctionnement la maintenance et l entretien de cet appareil Utilisez uniquement un bloc d alimentation qui est marqu CE ou la s curit certifi par un laboratoire reconnu au niveau national Le couvercle de s curit doit tre en place avant de brancher les fils d alimentation une alimentation lec trique Mettez tous alimentation contr le hors tension et d bran chez les cables d alimentation avant de retirer le couvercle de s curit Faire circuler que de l eau ou 50 50 eau thyl ne glycol dans l changeur de chaleur s il en est quip Ne branchez pas V changeur de chaleur a un robinet d eau ou toute autre source de liquide de refroidissement o la pression de l eau n est pas r glement Ne jamais introduire d antigel ou tout autre solvant orga nique dans n importe quelle partie de l instrument Solvants organiques provoquent des dommages irr parables Vappareil Ne faites pas fonctionner avec des temp ratures au dessus des tampons maximales sp cifi es sp cifications technigues Une surchauffe peut causer des dommages irr parables Vappareil Fan Sensor Kit e Attention Parce que cet instrument peut se d velopper de tension et une intensit suffisantes pour produire un choc mortel il faut tre prudent dans son fonctionnement Ce
13. authorized service center o p13 IMPORTANT Request a copy of the Hoefer Inc Health and Safety Declaration form before returning the item No items can be accepted for servicing or return unless this form is properly completed Note A Return Materials Authorization RMA number must be obtained from Hoefer Inc before returning any item to Hoefer Inc o p14 6 Customer Service Information 6 1 Technical Service and Repair Hoefer Inc offers complete technical support for all of our products If you have any gues tions about how to use this product or would like to arrange to repair it please call or fax your local Hoefer Inc representative Check the Hoefer Inc website at www hoeferinc com for the distributor in your area Or contact us directly at Hoefer Inc 84 October Hill Road Holliston MA 01746 Toll Free 1 800 227 4750 Phone 1 508 893 8999 Fax 1 508 893 0176 E mail support hoeferinc com Web www hoeferinc com 7 Disposal This equipment is marked with the crossed out wheeled bin symbol to indicate that it must not be disposed of with unsorted waste Instead it is your responsibility to dispose of your equipment correctly at life cycle end by handing it over to an authorized facility for separate collection and recycling It is also your responsibility to decontaminate the equipment in case of biological chemical and or radiological contamination so that the persons invol
14. bout 10 minutes if the TEMED volume is reduced to 100 ul and to approximately 15 minutes with 75 pl The amount of catalysts may need to be reduced under warm conditions Do not pour under direct sunlight The SQ33 Sequencer using 0 35 mm thick spacers requires approximately 60 mls of acrylamide solution 3 2 Gel Pouring D Remove any greasy fingerprints or water on the edges of the glass plates by wiping with acetone Lay the plain glass plate on a flat surface and arrange the spacers flush with the edges of the plate Carefully lay the notched glass plate on top and clamp the plates together The gel sandwich is now ready for pouring Refer to the illustration in Figure 1 page 6 Fill a 60 ml syringe with gel mix being careful not to aerate the solution Hold the syringe as vertical as possible and using a steady continuous motion expel the gel mix along the notched area moving the syringe nozzle repeat edly from one side of the notch to the other The gel mix should form a continuous pool along the top of the gel space and by capillary action it will move down between the plates Do not overfill the notched area the pool should be approximately half the height of the glass ears Alternatively do not let the pool run dry or air bubbles will be introduced between the glass plates The boundary of the gel should migrate as a straight line The gel plates can be tapped slightly behind the moving gel boundary usin
15. cable into the remaining socket on the rear of the temperature control box Select the temperature you require to run the gel at noting that while the control unit is factory calibrated there will be a temperature gradient across both the glass and aluminum plate Therefore it is necessary to set a temperature on the dial approximately 10 C lower than required at the gel plate e g if you require to run the gel at 55 C set the dial to approximately 45 C Attach the mains lead to the control unit and turn on the power The light on the control panel indi cates when the contacts are made for the fan opera tion When the light is on the fan will be off When the fan cuts in the light will go out The fan will automatically switch on off to maintain the gel temperature at 4 C of the set temperature After monitoring the gel temperature increase the dial setting as necessary A temperature indicator strip on the center of your glass plate will allow you to confirm that your dial setting is correct IMPORTANT Acrylic plastic is not resistant to aromatic or halogenated hydrocarbons ketones esters alcohol s over 25 and acids over 25 they will cause crazing especially of the UV transparent plastic and should not be used for cleaning Do not use abrasive creams or scourers Dry components with clean tissues prior to use 5 SQ33 Care and Maintenance e To remove the safety lid push thumbs down on
16. di corrente Utilizzare solo cavo elettrico danneggiato e attrezzature specifiche per le tensioni si intende utilizzare Tutte le appar ecchiature collegate ad alta tensione deve essere in accordo con EN61010 1 2001 o pv e Tenere lo strumento piu asciutto e pulito possibile Pulire regolarmente con un panno morbido e umido Lasciare asci ugare completamente lo strumento prima dell uso Non utilizzare lo strumento in alta umidita superiore all 80 Evitare la formazione di condensa lasciando che l unit raggiunga la temperatura ambiente quando prende lo strumento da un freddo a un ambiente piu caldo e Per consentire un sufficiente raffreddamento assicurarsi che le prese d aria dello strumento non sono coperti Viktig informasjon Norwegian SQ33 e Hvis dette utstyret brukes p en m te som ikke er spesifisert av Hoefer Inc beskyttelsen av utstyret kan bli svekket Dette instrumentet er beregnet for innendors laboratorium bruk Bare tilbehor og deler som er godkjent eller levert av Hoefer Inc kan brukes til drift vedlikehold og vedlikehold av dette produktet Bruk kun en str mforsyning som er CE merket eller sikkerhet sertifisert av et nasjonalt anerkjent testlaboratorium e Sikkerheten lokk ma vere p plass for du kobler til str m forsyningen forer til en stramforsyning Sl all str mtilf rsel styrer av og koble stromledningene for du tar sikkerheten lokket Sirkuler bare vann elle
17. er ethyleenglycol door de warmtewisselaar indien aanwezig Maak de warmtewis selaar niet aan op een waterkraan of een koelvloeistof bron waar het water zwak is niet gereguleerd Steek nooit antivries of organische oplosmiddelen op enig deel van het instrument Organische oplosmiddelen vero orzaakt onherstelbare schade aan het apparaat Werk niet met buffer temperaturen boven de maximale opgegeven technische specificaties Oververhitting vero orzaakt onherstelbare schade aan het apparaat Fan Sensor Kit Waarschuwing Omdat dit instrument kan voldoende spanning ontwikkelen en stroom naar een dodelijke schok produceren moet voorzichtigheid worden betracht bij de werking ervan Dit instrument is ontworpen in overeenstemming met de EN61010 1 2001 elektrische veiligheidsnorm Toch moet alleen worden gebruikt door goed opgeleid exploitanten Lees de volledige handleiding voordat u het instrument en gebruik alleen volgens de instructies Het instrument moet altijd gebruikt worden met de aard draad van het netsnoer goed geaard naar de aarde op het stopcontact e Gebruik alleen onbeschadigde elektrische draad en appa ratuur die specifiek zijn voor de spanningen die u wilt gebruiken Alle apparatuur die op hoge spanning moet in overeenstemming zijn met de EN61010 1 2001 Houd het instrument zo droog en schoon mogelijk Veeg regelmatig met een zachte vochtige doek Laat het instru ment volledig drogen voor gebruik
18. ertifierad av ett nationellt erk nt testlaboratorium S kerheten Locket m ste sitta p plats innan du ansluter str mf rs rjningen leder till en str mk lla Sl alla str mf rs rjningen kontrollerar av och kopplar leder innan du tar bort s kerheten locket Cirkulera endast vatten eller 50 50 vatten etylenglykol genom v rmev xlaren om s utrustad Anslut inte v rmev xlaren till en vattenkran eller n gon kylv tska k lla d r vattnet trycket r oreglerad Aldrig inf ra frostskyddsmedel eller organiskt l sningsmedel i n gon del av instrumentet Organiska l sningsmedel kan orsaka irreparabla skador p enheten Fungerar inte med buffert temperaturer ver de specifi cerade maximala tekniska specifikationer verhettning kan orsaka irreparabla skador p enheten Fan Sensor Kit Varning Eftersom detta instrument kan utveckla tillr cklig sp nning och str m f r att producera en d dlig chock m ste man vara f rsiktig i sin verksamhet Detta instrument r utformat i enlighet med EN61010 1 2001 els kerhet standard Det b r dock endast anv ndas av utbildad operat rer L s hela den h r handboken innan du anv nder instrumentet och endast anv nda enligt instruk tionerna e Instrumentet skall alltid anv ndas med jordkabel n tsladden korrekt jordad till jorden vid v gguttaget Anv nd endast oskadade elektrisk trad och utrustning som r specifik f r de sp nningar du kommer att anv nda
19. f the instrument are not covered Dulezit informace Czech SQ33 Pokud toto za zen pou vat zp sobem kter nen specifikov n Hoefera Inc ochrana poskytovan za zen m m e b t sn ena Tento p stroj je ur en pro vnit n laboratorn pou it Pouze p slu enstv a d ly schv len nebo dodan Hoefera Inc mohou b t pou ity pro provoz dr bu a servis tohoto produktu Pou vejte pouze nap jec zdroj kter je ozna en zna kou CE nebo bezpe nost certifikov na celost tn uzn vanou testovac laborato Bezpe nostn v ko mus b t na m st p ed p ipojen m nap jen vede k nap jen Zapn te v echny nap jen kontroluje a odpojte nap jec vodi e p ed vyjmut m bezpe nostn v ko Uv d ny do ob hu pouze vodu nebo 50 50 vody ethylen glykolu p es v m n k tepla pokud tak vybaven Nep ipojujte v m n ku tepla na vodovodn kohoutek nebo chladic zdroj kde je tlak vody neregulovan Nikdy zav st nemrznouc nebo jak koli organick rozpou t dla do jak koli sti p stroje Organick rozpou t dla zp sob nenapraviteln kody na za zen Nepracujte s n razn kov ch teplot ch nad maxim ln uveden ch technick ch specifikac P eh t m e zp sobit nenapraviteln kody na za zen Fan Sensor Kit Varov n Vzhledem k tomu tento n stroj se m e vyvinout dostate n nap t a proudu k v rob smr
20. g your fingers to prevent any bubble formation Do not use any hard or metal objects o When the gel boundary reaches the bottom of the glass plates remove the surplus gel from the notched area Failure to do so will result in the gel mix drip ping from the bottom end Insert the reguired comb O If you are using a comb with square wells insert the teeth without trapping bubbles If you are using a sharks tooth comb insert the flat face of the comb at a slight angle to prevent bubbles from trapped Add a few drops of gel mix if necessary Carefully straighten the comb so that it is parallel to the top of the gel plate and reaches 3 5 mm below the notched area Figure 1 Pouring gels horizontally e Hold syringe vertically e Move across notched area to maintain a pool of gel mix e Do not overfill e Do not attempt to force gel mix down between plates with the syringe jet e When gel boundary has reached the end remove surplus gel mix from notched area Leave just a small amount to ensure bubbles don t get trapped under the comb e The comb area may be clamped with bulldog clips e Do not let the clips protrude beyond the line of the comb otherwise get thick ness variation and hence lane distortion may occur e When gel has set carefully remove comb and insert it teeth first being careful not to damage points so it just contacts the gel surface e The gel is then ready to be attached to the main u
21. here the water pressure is unregulated Never introduce antifreeze or any organic solvent into any part of the instrument Organic solvents will cause irrepa rable damage to the unit Do not operate with buffer temperatures above the maxi mum specified technical specifications Overheating will cause irreparable damage to the unit Fan Sensor Kit e Warning Because this instrument can develop sufficient voltage and current to produce a lethal shock care must be exercised in its operation This instrument is designed in accordance with the EN61010 1 2001 electrical safety standard Nevertheless it should be used only by properly trained operators Read this entire manual before using the instrument and use only according to the instructions The instrument must always be used with the earth lead of the power cord correctly grounded to earth at the mains outlet Use only undamaged electrical wire and equipment specific for the voltages you will use All equipment connected to high voltage should be in accordance with EN61010 1 2001 Keep the instrument as dry and clean as possible Wipe regularly with a soft damp cloth Let the instrument dry completely before use Do not operate the instrument in extreme humidity above 80 Avoid condensation by letting the unit equilibrate to ambient temperature when taking the instrument from a colder to a warmer environment To permit sufficient cooling ensure that the vents o
22. imum buforow ych okre lonych specyfikacjach technicznych Przegrzanie mo e spowodowa nieodwracalne uszkodzenie urz dzenia Fan Sensor Kit Ostrze enie Poniewa instrument ten mo e rozwin wystarczaj ce napi cie i pr d do wytworzenia mierteln pr dem nale y zachowa ostro no w jej dzia ania Instrument ten zosta zaprojektowany zgodnie z EN61010 1 2001 elektryczne standard bezpiecze stwa Nale y jednak by u ywane tylko przez odpowiednio przeszkolonych operator w Przeczytaj ca instrukcj przed u yciem instru mentu i u ywa tylko zgodnie z instrukcj Urz dzenie musi by zawsze u ywane z uziemiaj cy prze wodu zasilaj cego prawid owo uziemione w gniazdku sieciowym U ywaj tylko nieuszkodzony elektrycznych przewod w i sprz tem dla napi b dzie u ywa Wszystkie urz dzenia pod czone do wysokiego napi cia powinny by zgodne z EN61010 1 2001 pvi Zachowaj instrument suche i czyste jak to mozliwe Przeciera regularnie miekka wilgotna szmatka Niech instrumentem wyschna przed u yciem e Nie u ywaj instrumentu w du ej wilgotno ci powy ej 80 Unikn kondensacji pozwalaj c urz dzenie wyr wnania temperatury otoczenia podczas wykonywania instrumentu z ch odniejszego do cieplejszego Aby umo liwi odpowiednie ch odzenie upewnij si e otwory w instrumencie nie s obj te Informa o importante Portuguese 5033 e Se e
23. items arrived If any part is missing contact your local sales office Inspect all components for damage that may have occurred while the unit was in transit If any part appears damaged contact the carrier immediately Be sure to keep all packing material for damage claims or to use should it become necessary to return the unit 1 2 SQ33 Sequencer Components Description Quantity Running Tank Lid Plain Glass Plates Notched Glass Plates 0 35 mm Melinex Spacers 0 35 mm Shark s Tooth Comb 48 well NINN m 2 SQ33 Specifications Unit Dimensions W x D x H Plate Dimensions W x H x D Standard Spacer Dimensions W x H x D Active Gel Dimensions W x H Maximum Sample Capacity Recommended Buffer Volume Recommended running conditions for 6 denaturing PAGE gel using passive ventilation at 20 C ambient temperature Recommended running conditions for 6 denaturing PAGE gel using FHS KIT at 20 C ambient temperature 2 1 Fan Sensor Kit Specifcations Temperature Control Unit Dimensions H x W x D Temperature Control Range Controller Accuracy Weight Fan Dimensions H x W x D Operating Speed Flow Rate Noise Rating 63 X 21 5 x 50 em 33 x 41 x 0 4 cm 10 x 410 x 0 35 mm 29 x 38 5 cm 1x 96 Upper Buffer Chamber 1200 ml Lower Buffer Chamber 1200 ml Power 45 55 W Gel Temperature 50 60 C Time 2 5 3 h Power 75 W Gel Temperature 55 C Time 1 75 h 80 x 96 x 140 mm O 100 C
24. kan anvendes til drift vedligeholdelse og service af dette produkt Brug kun en str mforsyning der er CE m rket eller sikker hedscertificeret med et statsanerkendt provningslabora torium e Sikkerheden L get skal v re p plads for du slutter str m forsyningen forer til en stramforsyning Drej alle stramforsyningen kontrollerer og frakobl stram forsyningen for du fjerner sikkerheds l g Cirkulere kun vand eller 50 50 vand ethylenglycol gennem varmeveksleren hvis monteret Tilslut ikke varmeveksleren til en vandhane eller en kolevaeske kilde hvor vandtrykket er ureguleret e Aldrig indf re frostv ske eller et organisk opl sningsmiddel til enhver del af instrumentet Organiske oplosningsmidler vil for rsage uoprettelige skader p enheden Betjen ikke med buffer temperaturer over de maksimale angivne tekniske specifikationer Overophedning vil for r sage uoprettelige skader p enheden Fan Sensor Kit Advarsel Fordi dette instrument kan udvikle tilstr kkelig sp nding og str m til frembringelse af en d delig chok skal der udvises forsigtighed ved driften Dette instrument er designet i overensstemmelse med EN61010 1 2001 elektrisk sikkerhed standard Ikke desto mindre b r det kun bruges af korrekt uddannet operat rer L s hele denne manual inden du bruger instrumentet og brug kun i henhold til instruktionerne e Instrumentet skal altid bruges sammen med jordledningen af netledningen
25. korrekt jordet til jorden ved stikkontakten Brug kun ubeskadigede elektriske ledninger og udstyr specifikt for de sp ndinger du vil bruge Alt udstyr der tilsluttes h j sp nding b r v re i overensstemmelse med EN61010 1 2001 Hold instrumentet s t r og ren som muligt T r regelm s sigt med en bl d fugtig klud Lad instrumentet t rre helt inden brug Brug ikke instrumentet i ekstrem luftfugtighed over 80 Undg kondens ved at lade enheden kvilibrere til stuetemperatur n r du tager instrumentet fra et koldere til et varmere milj At tillade tilstr kkelig k ling s rge for at ventilations b ningerne i instrumentet ikke er d kket Belangrijke informatie Dutch SQ33 e Als deze apparatuur wordt gebruikt op een manier niet bepaald door Hoefer Inc de bescherming die door de appa ratuur worden aangetast Dit instrument is ontworpen voor gebruik binnenshuis labo ratorium gebruik Alleen accessoires en onderdelen zijn goedgekeurd of geleverd door Hoefer Inc kan worden gebruikt voor exploitatie het onderhoud en reparatie van dit product Gebruik alleen een voeding die CE markering of de veiligheid gecertificeerd is door een nationaal erkende testlaboratorium De veiligheid deksel moet in plaats voordat u de voeding leidt tot een voeding e Schakel alle stroomtoevoer regelt af en haal de stekker leidt voordat u de veiligheid deksel Alleen circuleren water of 50 50 wat
26. l mp tiloissa enimm iskokoon tekniset tiedot Ylikuumeneminen korjaamatonta vahinkoa laitetta Fan Sensor Kit Varoitus Koska t m v line voi kehitty riitt v n j nnitteen ja virran tuottamiseen tappavan iskun on oltava varovaisia sen toiminnassa T m v line on suunniteltu mukaisesti EN61010 1 2001 s hk turvallisuus standardia On kuitenkin syyt k ytt vain asianmukaisesti koulutetut operaattorit Lue t m k ytt ohje ennen kuin k yt t laitetta ja k yt vain ohjeiden mukaan Laite on aina k ytett v maajohtoa virtajohto kunnolla maadoitettu Earth pistorasiaan K yt vain vahingoittumattomia s hk johdot ja laitteet spesifisi j nnitteiden k yt t Kaikki laitteet on kytketty suur j nnite olisi noudatettava EN61010 1 2001 Pid laite kuivana ja puhtaana kuin mahdollista Pyyhi s n n llisesti pehme ll kostealla liinalla Anna laitteen kuivua t ysin ennen k ytt l k yt laitetta hyvin kosteissa yli 80 V lt kosteuden tiivistymist antamalla yksik n tasaantua ymp rist n l mp tilaan kun otetaan instrumentti kylmempi l mpim mp n ymp rist n Sallia riitt v j hdytys ett tuuletusaukkojen v lineen eiv t kuulu Informations importantes French 5033 e Si cet quipement est utilis d une mani re non sp cifi e par Hoefer Inc la protection fournie par l quipement peut tre alt r e Cet instrument est con u pour une utilis
27. mbers in deionized water Never use organic solvents Dry the electrical connectors thoroughly with a tissue before storing the unit The kit consists of e 1 x 12 volt fan 4 securing screws e 1 x Self adhesive temperature sensor e 4 x Adhesive cable ties e x Temperature control unit 4 Installation and Operation of the Fan Sensor Kit 4 1 Installation D To fit the fan simply unscrew and retain the four screws securing the black ventilation cover on the safety lid Align the fan and note that the fan draws air up through the sequencing unit An arrow on the fan indicates the direction of the air flow Secure the fan into position using the long securing screws provided Replace the black ventilation cover on top of the fan so that the fan blades are not exposed Set up the polymerized seguencing gel in the unit Using the adhesive strip attach the flat face of the heat sensor to the center of the facing glass plate It is advisable to clean the area with a tissue soaked in acetone prior to attachment Do not allow acetone to come into contact with the plastic components O Route the heat sensor cable up and over the back corner of the gel unit securing the cable so that it will not drag on the sensor The fan controller is now ready for use o pll o p12 4 2 Using the Fan Controller D Attach the fan connector to the back of the tempera ture control box Attach the heat sensor
28. nit and loaded with samples Tap firmly with fingers over areas such as this Notched Area p6 O The gel polymerization should occur within 10 minutes but allow the gel to polymerize further for at least 90 minutes If it is to polymerize overnight enclose wet tissues under a cling film seal to prevent the ends of the gel from drying out If you are using a square toothed comb do not remove it until it is submerged below the running buffer However if you use a Shark s tooth comb it should be removed before the addition of buffer Insert it teeth first into the gel slot so that the teeth just come into contact with the gel and then add the running buffer 3 3 Unit Assembly O Place the lower buffer chamber into the main unit Push it back until it stops This ensures that the electrode separation and hence field strength are consistent from one run to another Remove any clamps and the bottom tape if used O Clamp the gel plate sandwich into position on the apparatus Do not over tighten the gel plate clamping screws as this will lead to the glass plates break ing and also making the insertion and removal of combs difficult o The gel plate assembly will rest against the aluminum back plate This has been set during manufacture to be parallel to the main frame Do not tighten or adjust the aluminum plate securing screws The aluminum plate is cushion mounted to provide good contact
29. r 50 50 vann etylenglykol gjennom varmeveksleren hvis slik utstyrt Ikke koble varmeveksler til en vannkran eller kjolevaeske kilde hvor vanntrykket er uregulert Aldri introdusere frostvaeske eller ethvert organisk opplos ningsmiddel inn i noen del av instrumentet Organiske losemidler vil fore til uopprettelig skade p enheten e Opererer ikke med buffer temperaturer over den maksimale angitte tekniske spesifikasjoner Overoppheting vil for rsake uopprettelig skade p enheten Fan Sensor Kit Advarsel Fordi dette instrumentet kan utvikle tilstrekkelig spenning og strom for produsere en dodelig stot m det utvises forsiktighet i sin drift e Dette instrumentet er utformet i samsvar med EN61010 1 2001 elsikkerhet standard Likevel bor det bare brukes av riktig opplaering operatorer Les hele denne h ndboken for du bruker instrumentet og bruk kun i henhold til instruk sjonene Instrumentet m alltid brukes sammen med jordledningen av stromledningen riktig jordet til jorden p stikkontakten Bruk bare uskadde elektrisk ledning og utstyr bestemt for spenninger du vil bruke Alt utstyr som er koblet til hoy spenning bor vaere i samsvar med EN61010 1 2001 Hold instrumentet sa t rt og rent som mulig Tork regelmes sig med en myk fuktig klut La instrumentet torke helt for bruk Ikke bruk instrumentet i ekstrem luftfuktighet over 80 Unng kondens ved la enheten tilpasse seg til omgivels estempe
30. raturen n r du tar instrumentet fra en kaldere til varmere omgivelser A tillate tilstrekkelig kj ling m du sorge for at ventilasjon s pningene p instrumentet ikke er dekket Wazne informacje Polish Q33 e Je li urz dzenie jest u ywane w spos b kt ry nie zosta okre lony Hoefer Inc ochrona zapewniana przez urz dzenie mo e by zaburzona e Instrument ten jest przeznaczony do u ytku w pomieszcze niach laboratoryjnych tylko Tylko cz ci i akcesoria zatwierdzone lub dostarczone przez Hoefer Inc mog by u ywane do obs ugi konserwacji i serwisowania tego produktu Nale y u ywa wy cznie zasilacza kt ry jest oznaczony znakiem CE lub bezpiecze stwa certyfikowane przez uznanym w kraju laboratorium Pokrywa bezpiecze stwa musi by na miejscu przed pod czeniem zasilania prowadzi do zasilania e W cz wszystkie Zasilacz kontroluje i od czy przewody zasilania przed zdj ciem pokrywy bezpiecze stwa Kr y wod lub 50 50 glikolu etylenowego wody przez wymiennik ciep a je li tak wyposa one Nie nale y pod cza do wymiennika ciep a do zaworu wody lub ka dego r d a ch odziwa gdzie ci nienie wody jest nieu regulowany Nigdy nie wprowadza zamarzaniu ani organicznych rozpuszczalnik w w jakiejkolwiek cz ci urz dzenia Rozpuszczalniki organiczne spowoduje nieodwracalne uszkodzenie urz dzenia Nie dzia aj w temperaturach powy ej maks
31. rective of October 27 1998 e 73 23 EEC LOW VOLTAGE DIRECTIVE IEC 1010 1 1990 plus AMENDMENT 1 1992 e EN 61010 1 1993 BS EN 61010 1 1993 89 336 EC THE EMC DIRECTIVE FOR ELECTROMAGNETIC COMPATIBILITY Hoefer single plate large format gel electropho resis units are designed to give long service and reproducible results in your laboratory A few moments spent reading these instructions will ensure that your expectations are reflected in the successful use of the apparatus First check that the apparatus has been received complete and undamaged following shipment Any faults or losses must be reported to Hoefer immediately Hoefer cannot accept responsibility for goods returned without prior notification Refer to the packing list and check that all components and accessories are present Please retain all packaging and materials until the warranty period has expired S SQ33 Function and Description The Hoefer SQ33 Seguencer offers a wide gel format for increased sample capacity and Shark s Tooth combs to ensure optimal band resolu tion The SQ33 Sequencer is perfect for DNA sequencing DNAse and RNase footprinting and heteroduplex or oligonucleotide analysis The removable lower buffer chamber combined with PRUT longer the upper buffer chamber drainage tap make buffer disposal easy and safe 1 1 Unpacking Unwrap all packages carefully and compare contents with the packing list making sure all
32. ste equipamento for utilizado de uma forma n o espe cificada pelo Hoefer Inc a prote o fornecida pelo equipa mento poder ser prejudicada Este instrumento foi projetado para uso em laborat rio no interior Somente acess rios e pe as aprovadas ou fornecidos por Hoefer Inc podem ser utilizados para a opera o manuten o e atendimento a este produto Usar apenas uma fonte de alimenta o que a marca o CE ou de seguran a certificado por um laborat rio reconhecido nacionalmente A tampa de seguran a devem estar no local antes de ligar o fornecimento de energia leva a uma fonte de alimenta o Vire toda a oferta de energia controla e desconecte os cabos de alimenta o antes de remover a tampa de seguran a Circulam apenas gua ou 50 50 de gua etileno glicol atrav s do permutador de calor se assim equipado N o ligue o trocador de calor para uma torneira de gua ou qualquer outra fonte de refrigerante onde a press o da gua regulamentada Nunca introduzir anticongelante ou qualquer solvente org nico em qualquer parte do instrumento Solventes org nicos podem provocar danos irrepar veis para a unidade N o opere com temperaturas acima de buffer o m ximo especificado especifica es t cnicas Superaquecimento ir causar danos irrepar veis a unidade Kit Sensor f Aten o Porque este instrumento pode desenvolver tens o e corrente suficiente para produzir um choque letal o
33. t c ok p e mus b t vykon v na v provozu Tento n stroj je navr en v souladu s EN61010 1 2001 Elek trick bezpe nost standardn Nicm n m lo by b t pou ito pouze dn vy koleni oper tor P e t te si celou tuto p ru ku p ed pou it m p stroje a pou vat pouze v souladu s pokyny P stroj mus b t v dy pou v n s zem veden m nap jec ho kabelu dn uzemn n na zem na z suvky Pou vejte pouze nepo kozen elektrick dr ty a za zen specifick ch pro nap t kter budete pou vat V echna za zen p ipojeno k vysok m nap t m by m la b t v souladu s EN61010 1 2001 Udr ujte p stroj jako such a ist jak je to mo n Ot ete pravideln m kk m vlhk m had kem Nechte p stroj doko nale vyschnout p ed pou it m Nepou vejte p stroj v extr mn vlhkost nad 80 Zabr nit kondenzaci par nechat p stroj vytemperovat na pokojovou teplotu p i vyjmut p stroje z chladn j ho do teplej ho prost ed e Chcete li povolit dostate n chlazen zajistit aby otvory pro p stroje se nevztahuje pii Vigtig information Danish Q33 Hvis dette udstyr bruges pa en made der ikke er specifice ret af Hoefer Inc den beskyttelse som udstyret kan v re veerdiforringet Dette instrument er beregnet til indendgrs laboratorium brug Kun tilbeh r og dele godkendt eller leveret af Hoefer Inc
34. t instrument est concu conform ment a la norme EN61010 1 2001 norme de s curit lectrigue N anmoins il doit tre utilis uniguement par des op rateurs d ment form s Lisez ce manuel avant d utiliser instrument et d utiliser uniguement selon les instructions Vinstrument doit toujours tre utilis avec le fil de terre du cordon d alimentation correctement mis la terre la prise de courant Utilisez uniquement des fils en bon tat lectrique et des quipements sp cifiques pour les tensions que vous allez utiliser Tous les quipements connect s haute tension doivent tre conformes la norme EN61010 1 2001 Gardez l appareil au sec et propre que possible Essuyez r guli rement avec un chiffon doux et humide Laissez instrument s cher compl tement avant utilisation e Ne pas faire fonctionner l instrument dans une atmosphere piv tr s humide plus de 80 viter la condensation en lais sant l unit s guilibrer la temp rature ambiante lors de la prise de instrument a partir d un froid un environnement chaud e Afin de permettre un refroidissement suffisant assurez vous que les orifices de l appareil ne sont pas couverts Wichtige information German SQ33 e Wenn dieses Ger t in einer Weise die nicht durch Hoefer Inc angegeben verwendet wird der Schutz des Ger ts beeintr chtigt sein Dieses Instrument ist f r den Innen Einsatz im Labor konzipiert Nur Zubeh r
35. t werden d rfen sondern separat behandelt werden m ssen Bitte nehmen Sie Kontakt mit einem autorisierten Beauftragten des Herstellers auf um Informationen hinsichtlich der Entsorgung Ihres Ger tes zu erhalten Questo simbolo indica che i rifiuti derivanti da apparecchiature elettriche ed elettroniche non devono essere smaltiti come rifiuti municipali indifferenziati e devono invece essere raccolti separatamente Per informazioni relative alle modalit di smantellamento delle apparecchiature fuori uso contattare un rappresentante autorizzato del fabbricante Este s mbolo indica gue el eguipo el ctrico y electr nico no debe tirarse con los desechos dom sticos y debe tratarse por separado Contacte con el representante local del fabricante para obtener m s informaci n sobre la forma de desechar el equipo Denna symbol anger att elektriska och elektroniska utrustningar inte f r avyttras som osorterat hush llsavfall och m ste samlas in separat Var god kontakta en auktoriserad tillverkarrepresentant f r information ang ende avyttring av utrustningen pix Warning These units are capable of delivering potentially lethal voltage when connected to a power supply and are to be operated only by qualified techni cally trained personnel Please read the entire operator s manual thor oughly before operating this unit These units comply with the statutory EC safety requirements e 98 79 EC In Vitro Diagnostic Di
36. tion of its products without prior notice This will enable us to implement developments as soon as they arise Hoefer Inc products are for research use only A return authorization must be obtained from Hoefer Inc before returning any product for warranty repair on a freight prepaid basis 8 1 Warning e Do not attempt to remove the outer casing or make repairs to our electrical range of products should any unit fail e Contact Hoefer Inc immediately if the need for repair or servicing should arise 8 2 Safety Precautions e Should the sensor develop a fault or become disconnected the fan will remain ON so safeguarding against gel overheating e The fan utilizes a brushless motor with reverse polarity protection e A 500 mAmp 240 Volt antisurge fuse is located on the rear panel in a fuse holder immediately below the mains supply socket o p17 9 Ordering Information Number Thickness Well width Well depth Well volume ul Product of wells mm mm mm per 1 mm depth Shark s Tooth Combs SQ33 C35 48K 48 0 35 3 10 5 SQ33 C35 60KMC 60 0 35 25 10 SQ33 C35 96K 96 0 35 2 5 9 3 5 Standard Combs SQ33 C35 48 48 0 35 4 5 7 SQ33 C35 60MC 60 0 35 2 5 3 5 Microtiter spacing for use with a multichannel pipette Additional Accessories and Replacement Parts Catalog number Glass Plates Rectangular 33 x 41 cm 2 pieces SQ33 GPLT Glass Plates Notched 33 x 41 cm 2 pieces SQ33 NGPLT Spacers 0 35 mm thick
37. und Teile zugelassen oder von Hoefer Inc versorgt kann f r den Betrieb verwendet werden Pflege und Wartung dieses Produkts Verwenden Sie nur eine Stromversorgung die CE Kennzeichnung oder die Sicherheit zertifiziert ist durch ein staatlich anerkanntes Pr flabor Die Sicherheit Deckel muss vorhanden sein bevor Sie die Stromversorgung f hrt zu einer Stromversorgung Schalten Sie alle Stromversorgung steuert aus und ziehen Sie den Netzstecker f hrt bevor Sie den Sicherheitsdeckel e Zirkulieren nur Wasser oder 50 50 Wasser Ethylenglykol durch den W rmetauscher falls vorhanden Schlie en Sie den W rmetauscher an einen Wasserhahn oder einen K hl mittel Quelle wo der Wasserdruck ist ungeregelt Nie einzuf hren Frostschutzmittel oder keine organischen L sungsmittel in einen Teil des Instruments Organische L semittel verursacht irreparable Sch den am Ger t Nicht mit Puffer Temperaturen oberhalb der maximal angegebenen technischen Spezifikationen arbeiten ber hitzung kann zu irreparablen Sch den am Ger t Fan Sensor Kit Warnung Da dieses Instrument gen gend Spannung entwickeln kann und Strom um einen t dlichen Strom schlag zu erzeugen muss darauf in seinem Betrieb ausge bt werden 2001 elektrische Sicherheit Standard Dieses Ger t ist in bereinstimmung mit der EN61010 1 ausgelegt Dennoch sollte sie nur von entsprechend geschultem Operatoren verwendet werden Lesen Sie das gesamte
38. va riconosciuto a livello nazionale Il coperchio di sicurezza deve essere in posizione prima di collegare l alimentazione porta ad un alimentatore Accendere tutti alimentatore controlla e scollegare i cavi di alimentazione prima di rimuovere il coperchio di sicurezza Circolare solo acqua o 50 50 di acqua glicole etilenico attra verso lo scambiatore di calore se presente Non collegare lo scambiatore di calore ad un rubinetto dell acgua o di qualsiasi altra fonte del liquido di raffreddamento in cui la pressione dell acqua regolamentata Non introdurre antigelo o qualsiasi solvente organico in qualsiasi parte dello strumento Solventi organici pu causare danni irreparabili alla centralina Non utilizzare con temperature sopra i massimi tampone specifiche tecniche indicate Il surriscaldamento pu causare danni irreparabili alla centralina Fan Sensor Kit e Attenzione Dal momento che questo strumento pu svilup pare una tensione e corrente sufficiente a produrre uno shock la cura deve essere esercitata nel suo funzionamento Questo strumento stato progettato secondo la EN61010 1 2001 standard di sicurezza elettrica Tuttavia deve essere utilizzato solo da parte di personale addestrato Leggere il presente manuale prima di utilizzare lo strumento e utiliz zare solo secondo le istruzioni Lo strumento deve essere sempre utilizzato con il cavo di terra del cavo di alimentazione di messa a terra a terra alla presa
39. ved in the disposal and recycling of the equipment are protected from any potential hazard For more information about where you can drop off your waste equipment please contact your local dealer from whom the equipment was purchased originally By doing so you will help to conserve natural and environmental resources and you will ensure that your equipment is recycled in a manner that protects human health Thank you o p15 o pl6 B Warranty Hoefer Inc warrants that the unit you have received has been thoroughly tested and meets its published specification This unit excluding all accessories is warranted against defective material and workmanship for a period of twelve 12 months from the date of shipment ex factory Hoefer Inc will repair all defective equipment returned during the warranty period without charge provided the equipment has been used under normal laboratory conditions and in accordance with the operating limitations and maintenance procedures outlined in this instruc tion manual and when not having been subject to accident alteration misuse or abuse No liability is accepted for loss or damage aris ing from the incorrect use of this unit Hoefer Inc s liability is limited to the repair or replace ment of the unit or refund of the purchase price at Hoefer Inc s option Hoefer Inc is not liable for any consequential damages Hoefer Inc reserves the right to alter the specifi ca
40. with the glass plate O Make sure that the upper buffer tank drainage tube Is closed O Attach the electrical connectors to the buffer cnambers IMPORTANT Do not fill over the maximum fill lines 3 4 Run Conditions and Buffer Volumes D Into the upper buffer chamber pour 800 ml approximately Into the bottom buffer chamber pour 800 ml approximately Ensure that there is no buffer leakage o If desired pre run the gel until the glass plates are warm Use the settings described below O Prior to loading your samples flush out the wells with running buffer to clear them of urea O Heat the sequencing samples at 95 C for 3 minutes place on ice and centrifuge 12 000xg for 3 minutes Return to ice D Load 1 5 ul of sample using a suitable loading tip If possible avoid taking the sample from the bottom of the tube particulate materials may cause streaking or smearing Try to minimize sample dispersion load the sample directly onto the bottom of the well and keep it as a thin layer O Replace the safety lid ensuring it is positioned fully down over the electrical connectors O Connect and run the gel at the desired power setting The leads and electrical connectors are tested to 1 500 Volts and users are advised not to exceed this voltage a Ou IMPORTANT Make sure that the bottom tank does not overflow Typically 45 55 Watts constant power will give good results
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