Digital PicoView® PV Acquire™ User manual
Contents
1. Interface Feature Performance Main Window Choose Settings Source Image or Diagnostics 7 2 1 Measurement Cursor User drag and drop placement for distance 7 7 measurement and_ establishing reference positions Cursor Readout Shows X Y distance mm between cursor 7 7 centers Image Controls Real Time Camera Exposure Gain Image 7 3 SSS WR Magnification and Image Tool controls 7 4 7 5 Sees Stage Control Panel Buttons enable precise XYZ jogging of stage 7 8 FIGURE 7 4 Primary PV Acquire Digital Divert Panel Remote contact closure or digital input 8 3 KR switching between spray and retract rinse position r Mode Panel Choose Tune Store Go To or Retract amp Home 7 2 2 Km Tabs for In depth control features eee U Pit 7 2 1 Settings Tabs misa ee The Settings tabs found at the top of the main window offer three levels of m LEE zj control Settings Source Image and Diagnostics Figure 7 5 ZE sa Main Window Button Feature Comment FIGURE 7 5 PV Acquire Settings tab Source Image Tab Exposure Gain Apply Controls Camera Exposure Time and Image Gain Source Image Tab Image Magnification Sets the Digital Image Magnification Value Source Image Tab Image Tool Selects Cursor Mouse Pan Mouse Zoom modes Source Image Tab Pan X Pan Y Sets image shift values when using Image Mag Values of 2 or 3X Settings Tab Acquisition Loop Time Controls refresh rate of source image from ca2. Divert Enable POE Divert Input Channel j m Channel 0 2 ON OFF Divert Channei1 Pivert When Input is sl True Closed Switch 4 FIGURE 8 3 Digital Divert configuration 5V plug me FIGURE 8 4 Digital Divert Interface Box REV 02115 1 A positive and negative wire should be connected to the Digital Divert box pins 1 and 4 Make sure that the wire running from 5V pin 1 runs to the positive terminal on the machine you re interfacing with 2 Connect the Digital Divert box to the top of the Digital PicoView source camera using the serial cable provided This provides the interface connection When connecting Digital Divert with Eksigent LC systems see Section 8 4 for specific contact closure instructions 8 3 2 Configuration Choices The Digital Divert interface gives a choice over the type of control contact closure or TTL compatible and the polarity of the interface The type of control is determined by the selected control channel Channel Selection Channel 0 is for conventional contact closure and requires an actual contact between pins 1 and 2 of the Interface Box The input is true when the contact is closed The Channel 0 LED will light when the contact is closed Channel 1 is for a TTL level compatible digital signal The input is true when the voltage between pins 3 and 4 of the interface box is approx 2 3 2 8 V or greater 5 V max The Channel 1 LED will light when the voltage is h
3. 5 1 Connect the USB cables Prior to using PV Acquire the USB connections between the host PC and Digital PicoView source need to be established This will be followed by the creation of a default configuration file using the PV Configure application 1 Connect the USB cable from the digital camera to the host PC Follow instructions to activate USB driver if required Figure 5 1 2 Connect the USB cable from the USB to serial converter to the host PC Follow instructions to activate USB driver if required Figure 5 2 5 2 Setting a Port Assignment for USB to Serial Adapter The USB to serial adapter will be assigned a virtual COM port number by the operating system This number needs to be determined and is used by the PV Configure application to establish a default settings file for PV Acquire 1 Open the Windows Device Manager In Windows XP this is typically found under Start gt Control Panel classic view gt System gt Hardware Tab See Figure 5 3 2 Click on the Device Manager button The Device Manager List will open Click on Ports COM amp LPT Look for USB Serial Port ComX in the sub list See Figure 5 4 4 Note the COM port setting e g COM3 COM4 etc that will be seen in parenthesis this assignment will be needed when running PV Configure for the first time 5 3 Selecting an Alternate COM Port OPTIONAL If the COM port setting chosen by the operating system is in conflict with another
4. 3 Note the position of the XYZ stage If a different Retract Position is desired type the new value s into Retract X Retract Y and or Retract Z 4 Click on Retract Tip to move to the new Retract Position Repeat steps 3 4 until the final desired position is obtained 6 Go to the Tune tab of the Mode panel and click Restore Tip to return the XYZ stage to the last operating position 7 12 Setting Home Position The Home Position is defined at the 0 0 0 setting of the XYZ stage The Home Position is an absolute reference position that both PV Acquire and the motor actuators use in making XYZ stage movements A Home Position operation must be performed when the Digital PicoView source is used for the first time A Home Position operation must also be performed if the electrical power to the motor actuators has been disrupted or disconnected If you are merely re starting PV Acquire and the power to the Digital PicoView source has remained intact a Home Position operation should not be required 1 Click on the Retract amp Home tab of the Mode panel 2 Click the Home Stage button The stage controls will gray out during the homing operation When the homing operation is finished the Stage Position will read O O 0 and a dialog window will appear confirming that the stage has been homed Figure 7 17 A The current operating position of the XYZ stage will be lost when the home position operation is performed 7 13 Quitting PV A
5. 6 1 PV Configure Overview PV Configure generates a default settings configuration file ini file that PV Acquire is required to open each time it runs Think of this much like a tune file for your digital source settings The default settings configuration file saves the following information COM port assignment e Number of the COM port for communication with the XYZ Stage through the USB to serial adapter XYZ Stage Direct Go to Position e Sets the default XYZ Stage position for the direct go to position XYZ Stage Retract Position e Sets the default XYZ Stage position for the retract command e Used as the retract position for Digital Divert Camera Exposure and Gain Settings e Sets start up values for image brightness and gain e Camera Acquisition Loop Time Sets the acquisition period in milliseconds for image acquisition Settings below the default value 102 mS may result in a slow down of the user interface The default value yields a frame rate 8 10 images sec e Cursor Calibration Cal X Cal Y Values Sets the mm calibration value in um pixel of the on screen cursors Value should ONLY be changed from default settings if the optical tube or lens is changed from standard configuration Cal X and Cal Y default values are 10 7 um pixel e Poll Stage Position Determines the frequency of polling the stage actuators for position This feature is associated with the manual control knobs on the stage control
6. 7 5 2 Image Zooming with the Mouse Zoom In and Zoom Out functions allow image display expansion and contraction 1 Select Zoom In from the Image Tool dropdown 2 Click in the image display To return to initial magnification click Zoom Out from the Image Tools dropdown or select 1X magnification to return the image to default settings 7 5 3 Panning Scrollbar The Pan X and Pan Y scrollbars in the lower left corner of the Source Image tab provide horizontal and vertical scrolling respectively Panning enables you to move the observable image space at 2X and 3X magnifications Unlike the mouse panning option Pan X and Pan Y scrollbar settings are saved when image magnification settings are changed 1 Select either 2X or 3X from Image Magnification A Click either the Pan X or Pan Y arrow and drag to the desired position Figure 7 10 The Pan Zoom In and Zoom Out options from the Image Tools dropdown and the Pan X and Pan Y scrollbars are only active at the 2X and 3X image magnifications 7 6 Saving Images The Source Image is readily captured with a copy and paste operation into another application such as Windows Paint The background image and cursors will be saved the cursor distance readout however is not 1 Right clicking in the image window will bring up a pop up menu 2 Select Copy Data Figure 7 11 Use a paste operation in another suitable application such as Paint or Microsoft Powerpoint 7 7 Using Me
7. REV 02115 mma oer Pin nen ee showing 9 Diagnostics amp Troubleshooting The error detection features of PV Acquire enable rapid trouble shooting of software and or hardware problems Errors are detected either through pop up dialog windows or in the Diagnostics tab of the main window Recovery from an error generally requires correcting the problem quitting from PV Acquire and re starting the application Fortunately recovering from such errors is often simple and does not require changing the emitter position or interruption of the ESI voltage The unique autonomous nature of the motor actuators of the Digital PicoView source ensures a crash of the PV Acquire software will not negatively affect your MS acquisition 9 1 No Image Visible in Window 1 In the Settings tab of the main window Check the Exposure 20 000 pS typically and Gain control 10 dB typically settings for non zero values 2 Check the Diagnostics tab If the camera is properly connected to the host PC all error codes should read zero All handle OUT variables will have positive values If the camera connection has failed the error code Dev Open or error code Get Dev will read back a negative value Driver Handle OUT will read zero Check the connection cable then quit and restart PV Acquire Simply reconnecting the camera cable without re starting PV Acquire will not correct the problem 9 2 Serial Connection Failure A failure of the serial c
8. MATAT YTTER riu sarl rrr EFA 2 U pour hardware came miih am installation OD o loppe disk invert it now hal do you wani lho eta la dy eh lratal fhe zofhasre eutomatically Mecommended CMratal kom a lit ta ipeci s bosko dw ch Lick Hid t Sort cese nos Cancel FIGURE 5 1 Found Hew Hardware Wizard Welcome to the Found New Hardware Wizard Window dl beach ha cumari red pdaled tolera by llarg un p w comecie on le hat wace natolat n CE Gr on Em racer Ligeia Wali e pasi ques rec PEH r PY B Lan window connect bo wirdcawr Lipote to resh l b fia 51 Ve ii tirs niy ied oe ra every bees comme a creme O Ma rt ta Chick Hesi to contrae ies _Concet_ FIGURE 5 2 System Properties System Restore Automatic Updates Remote General Computer Name Hardware Advanced Device Manager gt The Device Manager lists all the hardware devices installed gt on your computer Use the Device Manager to change the properties of any device x Device Manager Drivers pees Driver Signing lets you make sure that installed drivers are compatible with windows windows Update lets you set up how windows connects to windows Update for drivers Hardware Profiles lt lt Hardware profiles provide a way for you to set up and store Za different hardware configurations Hardware Profiles FIGURE 5 3 REV 02115 5 Software amp Hardware Initialization
9. NEW OBJECTIVE OPICOVIEW NANOSPRAY SOURCES PV ACQUIRETM POR AB SCIEX MASS SPECTROMETERS SOFTWARE MANUAL a NEW OBJECTIVE OPICOVIEW NANOSPRAY SOURCES PV ACQUIRETM FOR AB SCIEX MASS SPECTROMETERS SOFTWARE MANUAL REV 02115 REV 02115 1 OVERVIEW Overview 1 1 Introduction Welcome to the Digital PicoView Software Instruction Manual This manual describes the installation and operation of the PV Acquire Software module to be used with Digital PicoView hardware from New Objective Inc In addition to being a useful reference this manual can be utilized during user training Storing this manual near the computer operating your Digital PicoView is highly recommended 1 2 Software Overview The Digital PicoView software package consists of two main modules The first is PV Configure which is used to set up and configure a default settings file The second is PV Acquire which is the application that provides the graphical user interface for the source PV Acquire enables remote stage positioning high resolution spray imaging and advanced features such as Digital DivertiM This manual guides you through the installation of PV Configure and PV Acquire software and the source software communication hardware The sections guide you through the daily procedures of software initialization image optimization and tip positioning Your separate Digital PicoView Hardware Manual is your reference for source assembly hard
10. Starting PV Acquire PV Acquire can be re started with the procedure in section 7 1 However as long as the power to the Digital PicoView XYZ stage has remained intact from the last use there is no need to execute a Home Stage operation The user may safely decline the Home Stage request on re start see Figure 7 18 This way the stage position spray operation etc of the source will not be disturbed during the PV Acquire re start sequence PV Acquire may be exited and re started without disturbing the physical operation stage position spray voltage etc of the Digital PicoView source provided that a Home Stage operation is NOT performed on re start REV 02115 8 ADVANCED FEATURES 8 Advanced Features 8 1 Store Commands Store tab in the Mode panel contains functions for storing and re loading stage and cursor position files you select Figure 8 1 The position and cursor information is stored as a date and time stamped text file The name of the file may be changed to a title selected by the user 8 1 1 Save Cursor Position 1 Click on the Go To tab 2 Click Save Position to save the current emitter position and cursor setting in a position file 3 Click Load Position to load a position file that will restore the cursor and stage to the position contained in that position file Once the file is loaded the stage will move to the saved position and the stage will move to the new coordinates 8 1 2 Save Position 1 Click o
11. be used and must be discarded or repaired 2 2 2 Handling Fused Silica Tubing Handling of fused silica or glass tubing and tips can result in serious personal injury including eye and skin injury Use safety glasses or goggles meeting ANSI Z87 1 1989 requirements or the equivalent Puncture and chemical resistant gloves should 4 also be worn at all times 2 2 3 Tip Adjustment and or Replacement Do not attempt to adjust or replace the tip unless the ESI high voltage and other applicable voltages are turned off and are at ground potential A Reduce any applicable backing pressure liquid gas etc to ambient before loosening ESI tip fittings and removing the fused silica tip or transfer line from the coupling union Prior to pressurization make sure that components are tightened to specifications to prevent separation during use Failure to adhere to this warning could result in projectile like expulsion of the tip from the coupling union which could cause serious personal injury or damage to surrounding apparatus 2 2 4 The Transfer Line The transfer line connecting to either a tip module or the Micro Injection Valve must not be made from an electrically conductive material It must be fabricated from an electrically insulating material such as PEEKTM or fused silica Otherwise the operator may be exposed to potentially lethal voltage AN WARNING Do not defeat any mass spectrometer system software or hardware safety interlocks 2
12. every change in command state from false true Undesired changes in channel input state should be avoided especially if they occur on a time scale that is shorter than the transit time of the XYZ stage between positions When Digital Divert is activated all stage controls are grayed out and deactivated Additionally if manual knob control was activated this is also automatically turned off to prevent any unintentional stage movement 8 4 Configure the Digital Divert Interface Box The Digital PicoView Nanospray Source Hardware Manual contains information on installing and configuring the Digital Divert interface box Depending on the model the Eksigent system uses use either TTL or contact closure to communicate with the ion source The instructions below describe how to configure the Digital Divert box regardless of signaling method 1 Required Materials Parts supplied with the DPV 450 Digital PicoView Nanospray ion source e Digital Divert box e Power supply e DB 9 serial cable e Signal cable e Small screw driver e External interface cable Only when connecting to an ekspert nanoLC 400 system supplied with the LC system Digital PicoView source camera a ti eee Se wer e A a moe ss u mes a a Re sma s G McowiEwi FIGURE 8 6 Create a folder and name it apprpriately to save other time stamped files to The first green bar in Digital Divert will light up when the contact is c
13. 3 Trademarks The following trademarks are found in this instruction manual Teflon is a registered trademark of E l du Pont de Nemours and Co QTrap and TripleTOF are trademarks of AB Sciex MicroTight and SealTight are trademarks or registered trademarks of Upchurch Scientific Inc a division of IDEX Corporation PEEK and PEEKsil are trademarks of Victrex plc PicoView Digital PicoView PicoClear PicoTip PicoTip Powered and PicoFrit are registered trademarks of New Objective Inc IntegraFrit SilicaTip TaperTip GlassTip and are trademarks of New Objective Inc All other trademarks are the properties of their respective companies REV 02115 2 READ THIS FIRST 2 4 Limited Warranty DISCLAIMER Technical information contained in this publication is for reference purposes only and is subject to change without notice The information is believed to be reliable and accurate however nothing set forth herein constitutes a warranty of any kind or nature Given the variety of experimental conditions New Objective Inc cannot guarantee performance at a given flow rate the best guide to tip selection and operation is empirical testing The user is solely responsible for complying with any patent s pertaining to applications or methods using the products described or mentioned in this manual DISCLAIMER OF WARRANTIES New Objective warrants that PVAcquire Software Product will substantially conform to its specifications during the hardwar
14. ACY ARISING OUT OF OR IN ANY WAY RELATED TO THE USE OF OR INABILITY TO USE THE SOFTWARE PRODUCT EVEN IF NEW OBJECTIVE OR ANY SUPPLIER HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES AND EVEN IF THE REMEDY FAILS OF ITS ESSENTIAL PURPOSE Some states jurisdictions do not allow the exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you IVANVW 41SN MAIAOOld IVLISIG OS VAdd N 2 3 SOFTWARE COMPONENTS 3 Software Components 3 1 Host PC System Requirements 3 1 1 PV Acquire is compatible with the following operating systems Windows 8 Windows 7 Window XP Windows Vista Windows NT Windows 2000 The host PC is required to support two USB 2 0 or better connections 3 2 Software Checklist Digital PicoView Software Installation media All of the required application software device drivers dll s and the National Instruments Run Time Engine are on this drive Digital PicoView Operator s Manuals 3 3 Hardware Checklist Digital PicoView Nanospray Source with actuators amp camera installed See Digital PicoView Hardware Manual for configuration USB to serial port converter Serial cable for connecting actuators to converter USB Cable for camera to computer connection DPV 450 DIGITAL PICOVIEW USER MANUAL REV 02115 I 4 Software Installation The Digital PicoView software should be ins
15. Figure 7 1 3 Asecond dialog window will open asking if the XYZ stage requires a Home Stage operation The Home Stage operation is required if this is the first use of the stage or if power to the stage motors has been interrupted or disconnected Figure 7 2 A Home Stage operation must be performed when the Digital PicoView Stage is used for the first time OR If power to the unit s XYZ stage has been disrupted or disconnected 5 Click OK in the message box to begin using PV Acquire Figure 7 3 7 2 Overview PV Acquire is a powerful user interface for Digital PicoView enabling a high level of productivity for nanospray enabled mass spectrometry The user interface supplies an interactive digital image of the MS inlet and the necessary controls for XYZ stage positioning Advanced features such as Digital Divert enable the remote control of emitter XYZ position from other instruments such as autosamplers or liquid chromatographs One of the most useful features of the Digital PicoView platform is the smart XYZ motor controls used for stage actuation Each controller is an independent microprocessor driven device The Digital PicoView platform can be used without the PV Acquire application It also ensures that the stage position and spray performance will not be effected if PV Acquire is exited unexpectedly PV Acquire can be readily re started without effecting the stage position 7 PV ACQUIRE 450 APPLICATION
16. age This would only happen if your stage was moved using the manual control knobs not available on models after 2010 1 Click on the Settings tab 2 Click and hold the Poll Stage Position Every s drop down menu 3 Select a polling interval time of 5 10 or 30 seconds recommended 4 Click Apply You may verify automated polling operation by manually adjusting the stage position using one or more knob control s and waiting for the Stage Position window to update Using a short stage polling time 5 or 10 seconds can result in poor performance of the PV Acquire user interface on certain host PC s since portions of the interface are unavailable during the update position operation 7 PV ACQUIRE 450 APPLICATION 7 11 Setting Retract Position The Retract Position is typically used in two different operations 1 Moving the emitter far from the MS inlet making it easier to change emitters or clean the inlet etc 2 Establishing the emitter position for use with the Digital PicoView rinsing station The XYZ stage is retracted through the Retract Tip button from the Tune or Retract amp Home tab of the Mode panel The actual XYZ stage position of the Retract Position is set on the Retract amp Home panel Figure 7 15 1 Select on the Retract amp Home tab of the Mode panel 2 Click the Retract Tip button The stage will move to the settings set by Retract X Retract Y and Retract Z values The Stage Retracted LED will light up
17. andard dialog window will open The file may be saved under any name to any user selected directory 6 Note the file location and file name Click the OK button to save the file The PV Configure window will close automatically IVNNVW 41SN MAIAOOld 1TVLIDIQ OS VAdd Os N DPV 450 DIGITAL PICOVIEW USER MANUAL PV ACQUIRE 450 APPLICATION F s narw PY Setting File v Oe My Netwe Fim of how aa Fes v Cancel FIGURE 7 1 Renumbering Stage Units Left Right is First Unit In Out is Second Unit Up Down is Third Unit Home Stage Answer Yes if First use or power was out FIGURE 7 2 FIGURE 7 3 REV 02115 7 PV Acquire 450 Application 7 1 PV Acquire Start up PV Acquire requires user input of the location of the Configuration File each time it is started The XYZ stage also requires a Home Stage operation if this is the first time the software is connecting with the XYZ stage controllers i e installation or the power to the stage motors has been interrupted or disconnected The Home Stage operation sets the 0 0 0 point for XYZ stage motion Operation without establishing the proper Home position will result in erratic stage positioning and a loss of range of motion 1 Choose PV Acquire from the Start menu under the Digital PicoView heading 2 A dialog window asking for the location and name of the Configuration File created in section 6 2 will appear Choose the File and click OK
18. asurement Cursors There are two measurement cursors Blue Cursor GS1 gas nozzle cursor Green Cursor emitter cursor Cursors are placed with a drag and drop mouse operation using the left mouse button They are a powerful tool for establishing repeatable emitter protrusion from the GS1 gas nozzle Typically the blue cursor is placed at the front of the GS1 gas nozzle and the green cursor is placed at the tip of the emitter The Cursor Readout will then show the precise distance between the end of the gas nozle and emitter tip Use this value to adjust the tip protrusion from the gas nozzle Ideal protrusion distance is between 1 5mm and 2 0mm To position a cursor 1 Position the mouse cursor over either screen cursor The mouse cursor will change shape from a solid to hollow cross Note the blue cursor is a center marked line and should be grabbed at the center mark 2 Left click and hold the mouse while dragging the screen cursor to the desired position 3 Release the mouse button The Cursor Readout will show an updated distance measurement between the two cursors Figure 7 12 7 8 Stage Control Commands amp Parameters You can easily control XYZ position of the emitter tip using the Stage Control commands in the upper right corner of your screen The Stage Control contains two fields and six positioning commands Figure 7 13 Crash Limit defines the maximum forward tip Y distance um before colliding wit
19. cquire The proper way to quit PV Acquire is to always click Exit from the Tune tab of the Mode panel when closing PV Acquire Closing the software using other methods may leave background processes running and memory resources unavailable to other applications While other methods such as directly closing the program window through the Microsoft Windows buttons or using the application stop button will work to terminate the program it is not a best practice with PV Acquire Using the Exit button ensures that all PV Acquire functions are termintated properly and that saved settings will be retained 1 Click on the Tune tab in the Mode panel 2 ClickonExit A dialog box will appear asking ifyou want to update the default configuration file with the currently selected values Select Yes if you wish to 1 modify the existing configuration file or 2 create a new configuration file with the most recently used Digital PicoView operating parameters If you answer yes you will be prompted with a standard file dialog window 4 The only other way to exit the program is through the Windows Task Manager Control Panel CTL ALT DEL but this method is not recommended Always click EXIT before closing the PV Acquire window Closing the software using other methods may leave memory resources unavailable to other applications IVANVW 41SN MAIAOOld TVLIDIQ OS VAdd 7 PV ACQUIRE 450 APPLICATION DPV 450 DIGITAL PICOVIEW USER MANUAL M 7 14 Re
20. d PV Configure 12 A successful installation will also result in two icons being added to the desktop These icons point to applications for digital camera diagnostic software These pointers may be deleted from the desktop at no risk 13 Additional Installation Notes are available on the software USB drive follow these directions should the cdstarter exe application fail to run 4 SOFTWARE INSTALLATION 1 Wiecie t tha UNIT COTE NT St eral bo ceed TL Tir C vel Ti FIGURE 4 1 Confirm installation PV450 Installation Package 1 USB to Serial 3 PV Configure Installation Notes install install a 2 Camera install 4 PV Acquire Install FIGURE 4 2 Startup dialog box Gy Set Program Access and Defaults e Windows Catalog Windows Update Microsoft Update PY Acquire 450 2 0 0 PY Configure Programs 1 Documents 2 Settings k ya Search g Help and Support je Run Shut Down FIGURE 4 3 Two Digital PicoView applications are added to the START menu Windows XP shown FIGURE 4 4 Two additional icons will be added to MYDEWICECO the desktop upon installation These are not required and may e be deleted without risk WREDE IVANVW 41SN MAIAOOld 1TVLIDIQ OS Y Adq R DPV 450 DIGITAL PICOVIEW USER MANUAL I SOFTWARE amp HARDWARE INITIALIZATION Found Mee Hardware Wicard het wac rd hon cas mdd joder l
21. device the COM number may be assigned manually This may resolve serial port conflicts 1 Right click on the USB Serial Port ComX device in the Device Manager list and select Properties from the pop up menu Figure 5 5 Click on the Port Settings tab Click on the Advanced button Choose an alternate COM Port Number from the menu list Figure 5 6 Click the OK button in the Advanced Settings window P La ad Click the OK button in the Properties window 5 SOFTWARE 8 HARDWARE INITIALIZATION Prolific USB to Serial Comm Port COM3 Properties a General Port Settings Driver Dats Party None M sons j p red Seiling for CERP zz IVNNVW 41SN MAIAODld 1VLI91Idq 05y Adq On 2 6 PV CONFIGURE APPLICATION cos Serie COM Pori Choose Serial Fart El G R Chas COM 0 3 ward Serial Ports m Chasse COM FE a USB Serial Adapter COM taka pains HMO To es Des USE szoa r go l Dro eee Ports tour ni Control Paneles Suthers Piahana FIGURE 6 1 de lo o ur a i i mai ge me tel 21 RENE RE A p e EFI Kack Posiani H targem a miae E EL poz Bm ere p eee 6 m FIGURE 6 2 DPV 450 DIGITAL PICOVIEW USER MANUAL REV 02115 I i 6 PV Configure Application PV Configure must be run once prior to the first activation of PV Acquire The settings file created by PV Configure is required for the operation of PV Acquire
22. e period of the New Objective Digital PicoView product The warranty on any removable media used to distribute Software Product is provided in the applicable warranty statement TO THE MAXIMUM EXTENT PERMITTED BY APPLICABLE LAW NEW OBJECTIVE AND ITS SUPPLIERS HEREBY DISCLAIM ALL OTHER WARRANTIES AND CONDITIONS EITHER EXPRESS IMPLIED OR STATUTORY INCLUDING BUT NOT LIMITED TO WARRANTIES OF TITLE AND NON INFRINGEMENT ANY IMPLIED WARRANTIES DUTIES OR CONDITIONS OF MERCHANTABILITY OF FITNESS FOR A PARTICULAR PURPOSE AND OR LACK OF VIRUSES ALL WITH REGARD TO THE SOFTWARE PRODUCT Some states jurisdictions do not allow exclusion of implied warranties or limitations on the duration of implied warranties so the above disclaimer may not apply to you in its entirety LIMITATION OF LIABILITY Notwithstanding any damages that you might incur the entire liability of New Objective and any of its suppliers your exclusive remedy for all of the foregoing shall be limited to the greater of the amount actually paid by you separately for the Software Product or U S 5 00 or in country currency equivalent TO THE MAXIMUM EXTENT PERMITTED BY APPLICABLE LAW IN NO EVENT SHALL NEW OBJECTIVE OR ITS SUPPLIERS BE LIABLE FOR ANY SPECIAL INCIDENTAL INDIRECT OR CONSEQUENTIAL DAMAGES WHATSOEVER INCLUDING BUT NOT LIMITED TO DAMAGES FOR LOSS OF PROFITS OR CONFIDENTIAL OR OTHER INFORMATION FOR COST OF COVER FOR BUSINESS INTERRUPTION FOR PERSONAL INJURY FOR LOSS OF PRIV
23. h the mass spec inlet While the default limit is 12 500 um you can customize the value for each emitter Step Size is the incremental distance um traveled by the tip when you press any of the Stage Control buttons 7 PV ACQUIRE 450 APPLICATION pra Vesta pet rage R Em FIGURE 7 12 Measurement cursors provide precise reporting on distance between the two cursor positions Stage Stage Control Position In 12005 8 Y In Out 2805 81 Z Up Down 1433 12 Stage Retracted Crash Limit Step Size um 12500 Out 1000 FIGURE 7 13 Stage control panel IVANVW 41SN MAIAOOld IVLISIG OS V Add o DPV 450 DIGITAL PICOVIEW USER MANUAL gt N PV ACQUIRE 450 APPLICATION Tune Store GoTo Retract amp Home Update Manual Pos FIGURE 7 14 Tune tab In the Mode panel Tune Store GoTo Retract amp Home Retract X L R 2500 Home Stage Retract Y 1 0 Retract Tip 2500 FIGURE 7 15 Retract amp Home panel Retract Z U D 2 2500 Stage Position x Left Right 12005 8 Y In Out 2605 81 Z Up Down 1433 12 Stage Retracted FIGURE 7 16 m S age position settings FIGURE 7 17 Dialog window confirming that the stage has been reset to the home position Renumbering Stage Units Left Right is First Unit In Out is Second Unit Up Down is Third Unit Home Stage Answer Yes if First use or power was aut FIGURE 7 18 When restarting PV Acquire the rom
24. igh Divert when input is True will divert the XYZ stage to the retract position when the channel is true When the channel is false the XYZ stage will be in the spray position False will divert the XYZ stage to the retract position when the channel is false When the channel is true the XYZ stage will be in the spray position A The desired operating spray and retract rinse positions should be fine tuned before activating the Digital Divert feature 8 3 2 1 Using the Modified Digital Divert Box The modified digital divert box consists of a standard digital divert box which has been modified to be compatible with open collector inputs The modified digital divert box includes a 5 kOhms resistor which connects pins 1 and 3 and a jumper wire which connects pins 2 and 4 Connect the positive wire to pin 1 on the digital divert box and the negative or ground wire to pin 4 Select Channel 0 in the software under Divert Input Channel when using the modified Digital Divert box 8 3 3 Activating Digital Divert 1 From the main PV Acquire window select the ON Digital Divert radio button Figure 8 2 The Mode panel will gray out preventing access to other PV Acquire features 2 A window will pop up that asks you to create a file name this is for the automatic screen shots that will be taken at each position change We suggest creating a separate folder for these screen shots as the PV Acquire 8 ADVANCED FEATURES will aut
25. ill control the divert usually this is the elution channel b Connect the white wire to RUN Out of the same channel refer to Figure 8 9 rg gory nd FIGURE 8 9 Cable Connections on the nanolC Ultra NanolC or Tempo Systems e For a NanoLC or Tempo system a Connect the black wire to GND or CMN on the back of the LC Use the channel that will control the divert usually this is the elution channel b Connect the white wire to RUN Out of the same channel refer to Figure 8 9 IVWANVW 41SN MAIAOOld IVLISIG OS V Add 00 a DPV 450 DIGITAL PICOVIEW USER MANUAL I DIAGNOTICS amp TROUBLESHOOTING PEGVIEW ZZ gt FIGURE 9 1 Settings tab ir ia a is Er l A devine Pure OT s EE qe erze am cmon IRE int Era Ej Pemas ET el Bey or TE wey PEC Don Cao Ex CRETE rc Pas M y rie on dt Zaje LE Cigi Flm Path PE spears mi ngr pede atinga Me armasi stone Port Sagad lo E Y Corrales zem FIGURE 9 2 Diagnostics tab camera connections are stab showing all le FIGURE 9 3 Diagnostics tab USB cable interruption Hima ashap Daro wire Panda OUT dris tirir CT sx Are Pine czu lt w cmon E int AB b ETAT wa Trs Poi dace znow Dok lor b Ex CoE TE Ci dede qi PE Che Eigi Fle Fath Tiari ed ogn Ce PY Sabine le Corrente store Pot deped io k 1 2 Centrales F FIGURE 9 4 Diagnostics tab
26. ion Load Cursor Position Direct X Direct Y Direct Z Apply Retract X Retract Y Retract Z Retract Tip Home Stage Displays Raw Cursor information used for recalibration of Cal X Cal Y Values Readback only File path of the Configuration Settings File chosen at start up Readbcak only Value of the COM Port assignment Readback only Folder location for saving Digital Divert screengrab images Active field 7 2 2 Mode Panel Advanced Feature Controls Comment Moves stage from Current Position to the Retract Position as defined on the Retract 8 Home tab Moves stage from retract position to last used current position Preferred way to quit PV Acquire application Properly releases all memory resources and asks if update to settings configuration file is desired Software communicates with XYZ stage controllers Checks to see if stage has been moved using manual control knobs This features is inactive for systems after 2010 with no manual knobs Saves a position file to disk which contains the current XYZ stage position Loads a previously saved position file and moves the XZY stage to saved positions Creates and saves a cursor position file which contains the current cursor position Loads a previously saved cursor position repositioning the cursor to the loaded position Sets the coordinates for direct stage control Moves the Stage to position defined by Direct X Direct Y Di
27. le to communicate with the stage properly If this occurs start the Windows Task Manager CTL ALT DEL Locate and end the PV Acquire 1_ exe process It is recommended that after errors occur with PV Acquire the host PC is rebooted to prevent any additional downstream errors from occuring Repeat the process in Section 5 2 Setting a Port Assignment to determine the proper COM port assignment 9 4 PV Acquire User Interface Frozen The user interface can freeze or lock up if too many mouse clicks occur before the program can respond This is typically problematic when the camera s Acquisition Loop Time parameter found under the Settings tab in the main window is set below 50 mS The most efficient way to fully recover from lock up is to go to the Windows Task Manager end the PV Acquire 1_ exe process and re start the application It is recommended that after errors occur with PV Acquire that the host PC is rebooted to prevent any additional downstream errors from occurring 9 DIAGNOTICS amp TROUBLESHOOTING Error 1073807343 occurred at Property Node arg 1 in YISA Configure Serial Port Instr vi gt ZaberControlRefined isa vi gt Home XYZ_ SetRange_Offset_ ISA_All vi gt P Acq PY450 2_0_0 10 07 10 vi Possible reason s VISA Hex OxBFFFOO1 1 Insufficient location information or the device or resource is not present in the system Continue FIGURE 9 5 Error message for incorrect COM port assig
28. lers available on systems prior to 2010 e Knob Control Enables the user to toggle activation of manual control knobs on stage actuators available on systems prior to 2010 e Multiple PV Configure settings files may be used Each user can have one or more settings files to accommodate different nanospray configurations e All of the settings with the exception of the COM port value may be changed directly from within PV Acquire 6 PV CONFIGURE APPLICATION 6 2 Creating A Configuration Settings File 1 Select PV Configure from the Windows START menu under the Digital PicoView heading A dialog box asking you to select the proper COM port will pop up Figure 6 1 2 Select the COM port number from the menu selection as reported by the Device Manager in section 4 2 In this example the selected COM port is COM3 Do not click or type in this pulldown menu Doing so will corrupt the configuration file making PV Acquire unusable Select the Com Port from the pulldown menu only 3 The main PV Configure dialog window is now available 4 Select and modify each of the desired configuration parameters Guidelines for typical set up Direct Go To amp Retract default values are good starting points Camera Settings Exposure 20 000 uS Gain 10 dB Acquisition Loop Time 102 mS Poll Stage Position Every OFF Knob Control OFF Cal X Cal Y Default Values of 10 7 for each 5 Click Save to save the settings file A st
29. losed IVANVW 41SN MAIAOOld IVLISIG OS V Add 00 W 7 PV ACQUIRE 450 APPLICATION DPV 450 DIGITAL PICOVIEW USER MANUAL 00 R 2 Connect the Digital Divert box to the DPV 450 Digital PicoView nanospray ion source 1 Connect the DB 9 serial cable a Connect one end of the DB 9 serial cable to the digital camera b Connect the other end of the DB 9 serial cable to the back of the Digital Divert box 2 Connect the power supply to the side of the Digital Divert box 3 Connect the signal cable to the Digital Divert box a Connect the black wire to pin 4 b Connect the white wire to pin 1 FIGURE 8 7 Cable Connections on the Digital Divert Interface Box 3 Based on the LC configuration connect the other end of the signal cable e For a cHiPLC system regardless of the LC system a Connect the black wire to GND on the back of the cHiPLC system b Connect the white wire to VLV A refer to Figure 8 7 FIGURE 8 8 Cable Connections on the cHiPLC System Connectors e For an ekspert nanoLC 400 system Use the connector on the cHiPLC segment of the bifurcated external interface cable and follow the instructions above for the cHiPLC system refer to Figure 8 8 The connector does not have to be connected to anything it is just used to connect the wires REY 02115 7 PV ACQUIRE 450 APPLICATION e For a NanoLC Ultra system a Connect the black wire to CMN or GND on the back of the LC Use the channel that w
30. mera default value of 102 mS Settings Tab Stage Polling This feature is not active in later software versions v1 5x and later where the stage controllers have no manual knobs Controls how often PV Acquire communicates with Stage Controllers Used when lots of manual knob positioning is used Settings Tab Cal X Cal Y Cursor measurement calibration values typically these are NOT changed from default values Settings Tab Knob Controls Defeats the manul knob controls of the XYZ stage controllers This feature is inactive with hardware version later than 2010 Diagnostics Tab CCD Camera Communication and error values with Camera Readback only IVANVW 41SN MAIAOOld IVLIOIG OSVAdd A N 7 PV ACQUIRE 450 APPLICATION DPV 450 DIGITAL PICOVIEW USER MANUAL W Pieowiew FIGURE 7 6 Camera exposure setting too high FIGURE 7 7 Camera exposure optimized REV 02115 Diagnostics Tab Diagnostics Tab Diagnostics Tab Diagnostics Tab Mode Panel Tune Tab Tune Tab Tune Tab Tune Tab Store Tab Store Tab Store Tab Store Tab Go To Tab Go To Tab Retract amp Home Tab Retract amp Home Tab Retract amp Home Tab Cursor Data Configuration File Path Com Port Assignment Path to save Digital Divert sceengrab Button Feature Retract Tip Restore Tip EXIT Update Manual Pos Save Position Load Position Save Cursor Posit
31. n Loop Time mS Acquisition Loop Time controls the image refresh rate A low setting lt 50 mS can be used to monitor spray dynamics and is useful to observing transient spray behavior Settings below 100 mS can compromise the response time of the PV Acquire user interface and possibly lead to interface lock up 1 Click on the Settings tab in upper left corner of the screen 2 Click the arrow on the Acquisition Loop Time mS scale and drag to the desired position 100 mS works in most cases 3 Click Apply and note changes in image quality in the Image Preview screen on the right Continue adjustment to attain desired image clarity Decreasing Acquisition Loop Time increases image frame rate but lowers performance of the user interface 7 4 Image Magnification Magnifying the Source Image to 1X 2X and 3X provides digital magnification of fine image details Radio buttons located in the lower right corner of the Source Image screen allow rapid transition from one magnification level to another 1X 7 PV ACQUIRE 450 APPLICATION Exposure US Gain dB Pibirrrrinri I rurjrrrij Apply S000 500000 O 10 20 FIGURE 7 8 Camera acquisition controls Image Magnification Select Cursor zoom In Dix Oz Osx Zoom Cut FIGURE 7 9 Image tools drop down menu IVANVW 41SN MAIAOOld 1TVLIDIQ OS VAdd R A 7 PV ACQUIRE 450 APPLICATION DPV 450 DIGITAL PICOVIEW USER MANUAL Or FIGURE 7 10 Pan X and Pan Y scr
32. n the Store tab 2 Click Save Cursor Position to save the current cursor setting 3 Click Load Cursor Position to load a cursor position file that will restore the cursors to the positions contained in that previously saved position file Once the file is loaded the stage will move to the saved position and the stage will move to the new coordinates 8 3 Digital Divert Digital Divert is a powerful feature that enables remote toggling of the XYZ stage position between the spray and retract positions The feature saves both the spray and retract positions and your emitter can move between them with precision at a time interval of your choosing PV Acquire will take pictures of the spray at each interval for evidence of spray stability Control is via a low level intergace either a traditional contact closure or digital TTL compatible control signal with a third party hardware platform Another instrument mass spectrometer autosampler liquid chromatograph etc can then be used to control whether the nanospray emitter is in the operating spray position or the retracted rinse position The combined system ensures that both the mass spec inlet and emitter stay as clean as possible during repetitive LC MS MS runs For example some LC s provide a so called Run out signal that is actively true when a gradient is being delivered This can be used to enable Digital PicoView to position the emitter in the spray position during gradie
33. nment IVNNVW 41SN MAIAOOld TVLISIA OS VAdd O N 7 PV ACQUIRE 450 APPLICATION DPV 450 DIGITAL PICOVIEW USER MANUAL REV 02115 W 7 PV ACQUIRE 450 APPLICATION DPV 450 DIGITAL PICOVIEW USER MANUAL x o
34. nt elution and in the rinse position for loading column conditioning etc The Digital Divert interface is made available through the Digital PicoView source camera A cable and interface box connected to the camera provides the connection point for the interface Refer to the Digital PicoView Hardware Manual for instructions on making the physical interface connections and setting up the emitter rinsing station 8 3 1 Setting up Digital Divert The easiest way to connect Digital Divert to your MS is through an LC system Please check your LC manual for more information on connecting interfaces like Digital Divert Before activating your Digital Divert Box you should set the coordinates of your spray and retract wash positions the spray positions are in the upper right hand corner in the Stage Control section and the retract positions are in the lower right under the Retract amp Home tab See the hardware manual for more information If you are not interested in retracting your tip during spray but would like screen shots at intervals you choose simply match the coordinates of the retract position to those of the spray IVANVW 41SN MAIAOOld IVLISIG OS V Add 00 1 8 ADVANCED FEATURES DPV 450 DIGITAL PICOVIEW USER MANUAL 00 N Tune Store GoTo Retract 8 Home Load Position Load Cursor Position FIGURE 8 1 Digital Dive Divert Enable D E E e ON J OFF D FIGURE 8 2 a Toggle Digital Divert ON Digital Divert
35. oll bars Copy Data Description and Tip visible Items b Clear Graph Create Annotation lt Autoscale lt Autoscale Y AutosScale z Ignore Color Array Interpolate Color Smooth Updates FIGURE 7 11 Use the Copy Data feature to copy images to the clip board for use in other applications REV 02115 is the default magnification level To change magnification level click the corresponding radio button and wait for the image to refresh Figure 7 9 7 5 Image Tools Image Tools define available mouse modes when the mouse is placed over the image display Availability of Image Tools depends on magnification level At 1X magnification Select Cursor is the only active Image Tool Select Cursor transforms the mouse into a crosshair cursor allowing the user to move the green reference cursor in the Source Image display screen Additional tools activate upon selecting 2X or 3X magnifications These tools are grayed out at 1X magnification 1 Select a higher magnification level 2 Click the dropdown menu immediately below Image Tools and select desired tool Figure 7 9 7 5 1 Image Panning with the Mouse Pan converts the crosshair cursor to a hand to allow manual image panning Mouse panning position is reset each time the magnification is changed 1 Select Pan in the Image Tools dropdown 2 Place the hand cursor over image display 3 Click and drag to pull desired image components into view
36. omatically save other information about your Digital Divert toggling in similar formats After you create a method the system will take a screen shot at the very beginning of the method PEUT 3 Select Channel 0 or Channel 1 from the Divert Input Channel 1 drop down i aril menu Fig 8 3 Mu l 4 Select True or False from the Divert When Input Channel 1 is drop down menu arn y To camera 4 FIGURE 8 5 The Digital Divert Interface box connected to a cable that runs to the Digital Divert is now ready for operation 6 Digital Divert may be turned off at any time by clicking the OFF radio button 8 3 5 Testing Digital Divert 1 To test basic operation connect two 6 insulated wires with ends stripped 1 2 bare between pins 1 and 2 of the Digital Divert Control Interface Box Set Digital Divert ON and set to Channel 0 with positive True 3 Twist the two wires into electrical contact The Channel O LED should light green If the Channel O LED does not light check to see that the Control Interface Box power supply is plugged in The XYZ stage should begin a movement to the retract position The Stage Retract LED will then light green Disconnecting the wires should cause the Channel 0 LED to go dark with a return of the XYZ stage to the last used operating position 8 3 6 Digital Divert Control Buffer The Digital Divert interface input contains a memory buffer that will toggle the stage position for
37. onnection such as unplugging the cable to the XYZ stage controls or a power failure to the stage motors may result in a pop up error message showing a VISA session lost message Check all stage motor cable connections and motor power supply Correct any disconnects quit and then re start PV Acquire Sometimes a connection failure will NOT result in a message instead your stage will simply not respond to the commands you send it Make sure you check all stage connections before trying to move the stage Sending too many commands to a disconnected stage can cause the PV Acquire software to freeze If the software freezes open the Windows Task Manager ctrl alt del end the program and restart it 9 3 Incorrect COM Port Assignment If you disconnect and reconnect your XYZ stage from the USB port in your computer the computer may automatically reassign COM port numbers to the attached devices Adding another device may also reassign COM port numbers If there have been any device changes in your USB port you should confirm the number of the correct COM port for PV Acquire by disconnecting your XYZ stage and reconnecting it see section 5 2 for assigning port numbers Choosing an incorrect COM port assignment in PV Configure will result in painfully slow and repetitive at least six pop up window error messages similar to the following showing a VISA Timeout Expired message This error occurs because your PV Acquire program is unab
38. pt to home the stage can be declined if power to Digital PicoView has remained on REV 02115 The L and R arrows Left Right move the tip along the source X axis by an increment defined by the selected Step Size The In and Out arrows move the tip along the source Y axis by an increment defined by the Step Size The Up and Down buttons move the tip along the source Z axis by an increment defined by the Step Size Stage Position reports the current stage position Stage Retracted LED turns on green when the stage is in the Retract Positon 7 9 Tune Tab Commands Tune tab within the Mode panel contains basic emitter actuator positioning functions and allows you to properly exit PV Acquire Click the Tune tab in the Mode panel Figure 7 14 Retract Tip moves the emitter to the Retract X Retract Y Retract Z coordinates as set on the Retract amp Home tab of the Mode panel Restore Tip moves the emitter to the last established position before the retract button was pressed EXIT saves the configuration and safely quits PV Acquire Always click on the EXIT button when closing PV Acquire Closing the software using other methods may leave memory resources unavailable to other applications 7 10 Using Automated Stage Polling Inactive Feature PV Acquire can periodically poll the XYZ stage for its current position automatically if you are uncertain that the values reading in the software program match those of the st
39. rect Z Sets the coordinates of the Retract Position Moves the Stage to the Retract Position defined by Retract X Retract Y Retract Z Performs Home Stage Operation 7 3 Image Settings Expo sure Gain Acquisition Time The camera acquisition controls enable complete control over image quality enabling optimal spray inlet or emitter imaging If the Exposure setting is too low the Source Image will appear black If the setting is too high the image will be washed out Figure 7 6 Figure 7 7 depicts adjustments set for optimal camera exposure 7 3 1 Setting Image Exposure Time pS Exposure controls image quality with respect to image illumination just like the exposure time of a digital or film camera 1 Place the mouse over the arrow on the Exposure uS scale and drag to the desired position 50 000 100 000 us works in most cases Figure 7 8 2 Click Apply and note changes in image brightness Continue adjustment to attain desired brightness contrast 7 3 2 Setting Image Gain dB Gain controls image enhancement contrast range and noise level Increasing the gain allows for a shorter exposure time e g used to image spray dynamics but increases image noise 1 Click the arrow on the Gain dB scale and drag to the desired position 8 10 dB works in most cases Figure 7 8 2 Click Apply and note changes in image quality Continue adjustment to attain desired image clarity 7 3 3 Setting Acquisitio
40. talled and the host PC rebooted prior to making the USB connections between the Digital PicoView Camera XYZ actuators and host PC 4 1 PV Acquire Software Installation 1 Locate the Digital PicoView software Insert the software media USB thumb drive There will be two folders containing software appropriate for your OS one will be for 64 bit installation and the other for 32 bit installation Open the folder appropriate for your install 4 Locate the CDStarter exe file and double click to launch the installation application Accept the dialog box by clicking YES The start up window appears 6 Click on 1 USB to Serial Install to install the USB to serial converter driver Follow the default installation instructions 7 Click 2 Camera Install to install the camera drivers Follow the default installation instructions 8 Click 3 PV Configure Install to install the PV Configure application Follow the default installation instructions 9 Click 4 PV Acquire Install to install the PV Acquire application and National Instruments M Run Time Engine Follow the default installation instructions 10 Restart re boot the host PC The host PC must be rebooted to complete software installation Do not connect the USB cables from Digital PicoView Hardware prior to rebooting as this will cause software failure 11 Two Digital PicoView applications have been installed in the Windows START menu Digital PicoView gt PV450 Acquire 2_x_x an
41. ware configuration and specific nanoelectrospray applications Additional resources for nanoelectrospray applications can be found in the Tech amp App Notes section of the New Objective website http www newobjective com support IVANVW 41SN MAIAOOld TVLISIA OS VAdd I 2 READ THIS FIRST DPV 450 DIGITAL PICOVIEW USER MANUAL I 2 Read This First 2 1 Safety Considerations AN Electrospray ionization ESI involves the use of potentially lethal high voltage electrical current Observe all manufacturers safety recommendations in the use of such equipment No equipment modifications should be made except by trained personnel using methods approved by the manufacturer in accordance with all safety requirements Installation of equipment should be performed by qualified personnel in accordance with all applicable electrical codes Never use this product with defective damaged or faulty equipment Serious injury or death could result Only qualified personnel should use this product Provide a safe workplace equipped with all necessary safety equipment 2 2 1 Prior to Installation Follow all safety recommendations of the equipment manufacturer s All system voltages must be brought to ground potential and all high voltage contacts disconnected from the inlet system before installation of the Digital PicoView system Inspect all equipment carefully prior to use Any damaged chipped or cracked components should not
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