Applied Biosystems Prism 377 with 96
Contents
1. GR1190 GR1189 y gt Distance between needles 9mm in closed position 4 10 8 mm in open position Gels 2 7 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Suggested The following table lists the suggested load volumes and sample Sequencing Load resuspension volumes for sequencing Volumes Resuspension Loading Vol No of Wells Vol uL a pL 24 36 6 9 1 5 48 2 4 1 0 1 5 64 2 4 1 0 1 5 96 2 4 1 0 1 5b a 5 1 Deionized formamide to 50 mg blue dextran mL in 25 mM EDTA b Loading 1 5 uL requires a syringe with a 0 2 mm tip to facilitate loading at the bottom of the well WARNING CHEMICAL HAZARD Formamide is a known teratogen It can cause birth defects Wash thoroughly after handling formamide Wear appropriate protective eyewear clothing and gloves Obtain a copy of the MSDS from the manufacturer Wash thoroughly after handling formamide Suggested The following table lists the suggested load volumes for GeneScan GeneScan Load Volumes Loading Vol No of Wells uL 24 36 1 5 50 1 0 1 5 66 0 5 1 0 96 1 0 1 5a a Loading 1 5 uL requ2. Step Action 1 Exit Collection 2 Press the reset button on the back of the 377 instrument 3 Immediately press the reset button again After a total reset a new copy of the firmware will be downloaded to the instrument when you relaunch the ABI PRISM 377 96 Collection software 3 6 Software and Firmware Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting Overview In This Chapter The following topics are covered in this chapter Topic See Page Gels 4 2 Thermistors 4 3 Results 4 3 377 96 Error Messages 4 4 Glass Plates 4 6 Troubleshooting For more information on troubleshooting refer to the following manuals References 4 GeneScan Reference Guide ABI Prism 377 DNA Sequencer User s Manual Troubleshooting 4 1 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting Gels Problem Possible Cause Solution Leaking wells Loose combs Sequencing Electrophorese immediately then after each three loads with the eight channel loader GeneScan Leaking wells are not tolerated in GeneScan applications If a well leaks it is best to run another gel At the very least do not use the wells around the leaking lane Bad clamps Be sure to use three bulldog clamps P N 4305386 with 10 12 lbs clamping pre
3. A rtisan Artisan Technology Group is your source for quality Technology Group new and certified used pre owned equipment FAST SHIPPING AND SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT DELIVERY Experienced engineers and technicians on staff Sell your excess underutilized and idle used equipment TENS OF THOUSANDS OF at our full service in house repair center We also offer credit for buy backs and trade ins IN STOCK ITEMS www artisantg com WeBuyEquipment 7 EQUIPMENT DEMOS HUNDREDS OF Instra e REMOTE INSPECTION LOOKING FOR MORE INFORMATION MANUFACTURERS Remotely inspect equipment before purchasing with Visit us on the web at www artisantg com 7 for more our interactive website at www instraview com information on price quotations drivers technical LEASING MONTHLY specifications manuals and documentation RENTALS ITAR CERTIFIED CEP BAD tia Contact us 888 88 SOURCE sales artisantg com www artisantg com ABI Prism 377 DNA Sequencer 96 Lane Upgrade User s Manual BS 28 ms Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Copyright 2000 Applied Biosystems For Research Use Only Not for use in diagnostic procedures ABI PRISM and the ABI PRISM design AmpliTaq GeneAmp and GeneScan are registered trademarks of PE Corporation or its subsidiaries in the U S and certain other countries ABI AFLP Plant Mapping AmpF STR AmpF STR
4. 105 mg Fluorescent NHS For post synthesis labeling of primers containing a 5 Aminolink 2 Esters P N Item Quantity 400981 TAMRA NHS Ester 5 mg 60 uL in DMSO 400980 ROX NHS Ester 5 mg 60 uL in DMSO 400808 Aminolink 2 0 25 g C 4 Part Numbers Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Matrix Standard Kit Dye Primer Matrix Standards Kit Filter Set A for NHS ester labeling Contains one tube each of 5 FAM JOE TAMRA and ROX labeled DNA IFJANTP matrix standards Contains one tube each of R110 R6G TAMRA and ROX labeled DNA Fluorescent Amidite Matrix Standards Kit Filter Set C for fluorescent phosphoramidite labeling Contains one tube each of 6 FAM TET HEX TAMRA and ROX labeled DNA NED matrix standard Used in combination with the 5 FAM JOE and ROX dyes in the Dye Primer Matrix Standards Kit or the 6 FAM HEX and ROX dyes in the Fluorescent Amidite Matrix Standards Kit Sets P N 401114 402792 401546 402996 Fluorescent Genotyping P N Kit Demonstration 402246 Kits A and B Kit A PCR reagents Contains six fluorescent labeled PCR primer pairs labeled with HEX TET amp FAM two control DNAs CEPH 1347 02 and 1347 10 and a ready made mix of PCR reagents containing AmpliTaq Gold DNA Polymerase GeneAmp PCR Buffer lI dNTPs and magnesium
5. Run 36F 2400 GS Run 36F 2400 CHILLER Plate Check A Plate Check A CHILLER Plate Check C Plate Check C CHILLER Plate Check D Plate Check D CHILLER Plate Check E Plate Check E CHILLER Plate Check F Plate Check F CHILLER Seq PR 36A 1200 Seq PR 36A 1200 CHILLER Seg PR 36A 2400 Seq PR 364 2400 CHILLER GS Run 36D 1200 CHILLER Seq Run 36A 1200 Seq Run 36A 1200 CHILLER Seq Run 36A 2400 Seq Run 36A 2400 CHILLER Seq Run 36E 1200 Seq Run 36E 1200 CHILLER Seq Run 36E 2400 Seq Run 36E 2400 CHILLER Seq Run 48A 1200 Seq Run 48A 1200 CHILLER Seq Run 48B 1200 CHILLER Seq Run 48E 1200 Seq Run 48E 1200 CHILLER GS Run 60W D CHILLER GS Run 2140V A CHILLER GS Run 2140V C CHILLER GS Run 2140V D CHILLER GS Run 60W A CHILLER GS Run 60W C CHILLER a PR Prerun Seq Sequencing GS GeneScan Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Firmware Collection The 96 Lane Scan mode uses 480 collection channels per scan up Channels from 388 in an XL Scan mode This provides a 5x oversampling for Increased analysis Position Based Due to the increased demand for positional accuracy of the detection Integration optics a new integration scheme is used Previously a given time was Scheme given to each channel before reading the CCD camera and switching
6. labeled primer 40 pmol of TAMRA and ROX labeled primer and a control template in quantity enough for 50 ss or ds DNA sequencing reactions P N Primers 401131 21 M13 Dye Primers 4 x 50 5 TGT AAA ACG ACG GCC AGT 3 401130 M13 Reverse Dye Primers 4 x 50 5 CAG GAA ACA GCT ATG ACC 3 401127 T7 Dye Primers 4 x 50 5 TAA TAC GAC TCA CTA TAG GG 3 401128 T3 Dye Primers 4 x 50 5 ATT AAC CCT CAC TAA AGG GA 3 401129 SP6 Dye Primers 4 x 50 5 ATT TAG GTG ACA CTA TAG 3 402787 SK Dye Primers 4 x 50 5 CGG CCG CTC TAG AAC TAG TGG ATC 3 402786 KS Dye Primers 4 x 50 5 CCT CGA GGT CGA CGG TAT CG 3 403013 PI Dye Primers 4 x 50 5 CAG GAC ATT GGA TGC TGA GAA TTC G3 403014 PI Dye Primers 4 x 50 5 CAG GAG CCG TCT ATC CTG CTT GC J Matrix and Sequencing P N Standard Standards 403047 dRhodamine Matrix Standards Kit 401114 Dye Primer Matrix Standards Kit 401071 Dye Terminator Matrix Standards Kit 401920 Dye Primer Cycle Sequencing Standard 402830 Dye Terminator Cycle Sequencing Standard 4303120 dRhodamine Terminator Cycle Sequencing Standard 4304154 BigDye Terminator Cycle Sequencing Standard C 10 Part Numbers Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Application Kits P N Kit 4303557 HLA A Sequencing Based Typin
7. to the next channel This release of firmware introduces position based CCD integration where predetermined stage positions determine when to switch Expanded Scan The 96 Lane Scan mode uses a larger scan region to accommodate Region the increased comb size The stage travels farther towards the edge on each side and accelerates at a faster pace as it reenters the scan region This offsets the increased number of collection channels so that the integration time per channel remains approximately that of an XL scan As a result there is no loss of sensitivity for a 96 lane scan compared to an XL scan The instrument firmware automatically adjusts the size of the read region according to the selected scan mode Users who wish to prevent the firmware from redefining the size of the scan window may be provided with special module files for this purpose Software and Firmware 3 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Reloading the If the instrument does not respond to commands or responds Firmware inappropriately the firmware image may be corrupted You can reset the firmware by performing a total reset also known as a double reset Performing a Total Reset A total reset erases the current firmware image from instrument memory This is indicated by the instrument status lights changing from green ready to flashing yellow To reset the firmware image with a total reset
8. 3 Reverse 5000 P N Kit Reactions 4303573 Ready Reaction 24 4303149 Ready Reaction 100 4303150 Ready Reaction 1000 4303151 Ready Reaction 5000 P N Primer Reactions 402111 21 M13 100 402109 M13 Reverse 100 Dye Primer Cycle Sequencing Core Kits with AmpliTaq DNA Polymerase FS Dye Terminator Kits with AmpliTaq DNA Polymerase FS Core Kit configurations contain all essential reagents packaged in separate tubes Each kit sequences both single stranded and double stranded templates P N Primer Reactions 402071 21 M13 100 402072 M13 Reverse 100 402073 21 M13 M13 Reverse 100 402125 Kit reagents only primerless 100 402126 T7 100 402127 T3 100 402128 SP6 100 402129 T7 SP6 100 402130 T3 T7 100 402799 SK 100 402798 KS 100 402797 SK KS 100 Dye Terminator Kits sequence single stranded and double stranded Cycle Sequencing templates Ready Reaction formulations contain all necessary reagents in one stable premix The Core Kit configuration contains all essential reagents packaged in separate tubes P N Kit Reactions 402123 Ready Reaction 24 402080 Ready Reaction 100 402119 Ready Reaction 1000 402124 Ready Reaction 5000 402118 Core Kit 100 Part Numbers C 9 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Dye Primers Kits include 20 pmol of FAM and JOE
9. 95 TAMRA dUTP 24 nmol 2 x 30 uL 402793 FJdCTP Set R110 R6G 3 3 and 12 nmol 3 x 30 uL and TAMRA 402795 R110 ACTP 6 nmol 2 x 30 uL 402796 R6G dCTP 6 nmol 2 x 30 uL 402794 TAMRA dUTP 24 nmol 2 x 30 uL a TAMRAJdNTP is supplied at a concentration four times higher than R110 dNTP and IR6EGJANTP because it produces approximately four times less signal Part Numbers C 3 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Fluorescent ANTP Each kit listed below includes a GeneAmp kit as specified 100 PCR Kits reactions along with an F dNTP set that contains 30 uL each of R110 dNTP 3 nmol IREGJANTP 3 nmol and TAMRA dNTP 12 nmol P N Kit N808 0220 GeneAmp PCR Reagent Kit with AmpliTaq DNA Polymerase with F dUTP Set N808 0221 GeneAmp PCR Core Reagents with FJdUTP Set N808 0222 GeneAmp Thermostable r7th Reverse Transcriptase RNA PCR Kit with F IdUTP Set N808 0223 GeneAmp PCR Reagent Kit with AmpliTaq DNA Polymerase with F JdCTP Set N808 0224 GeneAmp PCR Core Reagents with F JdCTP Set N808 0225 GeneAmp Thermostable r Tth Reverse Transcriptase RNA PCR Kit with F JdCTP Set Fluorescent For direct 5 end labeling on an automated DNA synthesizer Phosphoramidites P N Item Quantity 401527 6 FAM Phosphoramidite 85 mg 401533 TET Phosphoramidite 100 mg 401526 HEX Phosphoramidite
10. Contact the companies listed for availability pricing and technical information regarding these products Suppliers Inside the U S Supplier Supplier Headquarters Product Part No Kloehn 10000 Banburry Cross Dr Loader 0 25 mm 18597 Company Las Vegas NV 89134 USA Loader 0 3 mm 18663 Voice 702 243 7727 Fax 702 243 6036 Needle 0 25 mm 18597 World Wide Web http www kloehn com 8 Outside U S offices are listed on the Needle 0 3 mm 18628 following page 8 Two Pitch Eight Channel Loader Suppliers B 1 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Supplier Supplier Headquarters Product Part No World Precision Sarasota International Trade Center Loader Gel Mate 96 Instruments Inc 175 Sarasota Center Blvd Sarasota FL 34240 9258 USA Voice 941 371 1003 Fax 941 377 5428 World Wide Web http www wpiinc com Needle 0 25 mm 67124 10 Outside U S offices are listed on the following page Note Hamilton Co 702 858 3000 also supplies loaders that may work with this upgrade Suppliers Outside the U S Supplier Supplier Contact Geographic Areas Served Kloehn Europe Bahnhofstrasse 12 Europe Postfach 55 CH 7402 Bonaduz Switzerland Voice 41 81 630 2303 Fax 41 81 641 3488 E mail kloehneurope bluewin ch World Precision P O Box 1191 Australia Instruments In
11. N Kit Contents 377 96 66 80B 377 96 90B 377 96 90C 377 96 120C 377 96 377 96 XL Kit stepped plates 36 cm pair of spacers 4305693 2 2 2 Disk 377 Collection s w v 2 5 4305535 Manual user s 96 lane upgrade 4305423 Clamps glass 2 in 4305386 Comb 100 well shark s tooth cast 0 4 mm 1 8 mm center 4305385 Kit EEPROM 96 lanes PCA tested 16 MHz 377XL Upgrade 8 MB Power Mac RAM SIMM Upgrade 8 MB Power Mac RAM DIMM Mac OS 8 4 1 Seq Anal 3 2b 4 4 GeneScan 3 0 4 4 a Spacers are 48 cm and need to be cut to size before use b As licensed Note The 96 lane upgrade also includes hardware modifications that will be made by the service engineer at installation l 4 Introduction Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Gels Overview In This Chapter The following topics are covered in this chapter Topic See Page Preparing Gels 2 2 Setting Run Conditions 2 5 Loading Gels 2 7 Gels 2 1 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Preparing Gels Pouring Gels To pour gels for use on the ABI PRISM 377 96 lane DNA sequencer Step Action 1 Cast the gels as instructed in the AB Prism 377 DNA
12. PRISM 377 DNA Sequencer Parts C 1 ABI PRISM DNA Fragment Analysis Kits and Reagents C 3 ABI PRISM DNA Sequencing Kits and Reagents C 8 User s Manuals cios id C 12 Part Number Updates 2 5 peser eee nese ewer bee etere pea es C 12 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Introduction Overview About This Manual Technical Support Contacts In This Chapter This manual describes the enhancements to the ABI PRism 377 DNA Sequencer included in the 96 lane upgrade Be sure to place this manual in your ABI PRISM 377 DNA Sequencer User s Manual For technical support contact information refer to the ABI PRISM 377 DNA Sequencer User s Manual The following topics are covered in this chapter Topic See Page Upgrade Overview 1 2 Important Upgrade Notes 1 3 Kit Configurations 1 4 Introduction 1 1 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Upgrade Overview Product Overview The ABI PRISM 377 DNA Sequencer 96 lane Upgrade Kit enhances the capabilities of the 377 DNA sequencer to support up to 96 lanes for both Sequencing and GeneScan applications Key Features The following list provides an overview of the key features of the 96 lane upgrade Increased scan window by 20 allowing additional lanes to be added without losing sensitivi
13. Profiler AmpF STR Profiler Plus ApliTaq Gold Applied Biosystems BigDye Primer BigDye Terminator and True Allele are trademarks of PE Corporation or its subsidiaries in the U S and certain other countries All other trademarks are the sole property of their respective owners Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Contents Introduction 0 0 0 0 ccc cc ecw ee ee eee ee eee L I NA 4 opie l es pha pinay Me RR ao ROEA 1 1 Upgrade Overview 0 0 ec cir et si edds teette ati 1 2 Important Upgrade Notes 02 0 0 eee eee eee eee 1 3 Kit Configurations essee reese eeste seet eee eee eee 1 4 Ca al OVERVIEW A En II nase Oe nes dl Reason ee ae we es 2 1 Preparing Gel cocaina Goon ba eben ba vide be 2 2 Setting Run Conditions 0 0 0 0 0 cee eee eee 2 5 Loading Gels xs ik 2 7 Software and Firmware oo oooooooo o 3 1 OVERVIEW il nia qe 3 1 NO ss sis seb ae a ta Sa a ID ie ea 3 2 Pd iaa 3 5 Troubleshooting o oooooooooomm m m o HI ONCE Witt dido 4 1 Troubleshooting srece mA 4 2 Filter Set Dye Combinations A 1 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com B Two Pitch Eight Channel Loader Suppliers B 1 Supplier Information Tables 0 0 cece eee eee ee B 1 C Part Numbers ccc cece cece cc cceee Cul ABI
14. R GS PR 12F 2400 GS PR 12F 2400 CHILLER GS PR 36A 1200 GS PR 36A 1200 CHILLER GS PR 36A 2400 GS PR 36A 2400 CHILLER GS PR 36C 1200 GS PR 36C 1200 CHILLER GS PR 36C 2400 GS PR 36C 2400 CHILLER GS PR 36D 1200 GS PR 36D 1200 CHILLER GS PR 36D 2400 GS PR 36D 2400 CHILLER GS PR 36E 1200 GS PR 36E 1200 CHILLER GS PR 36E 2400 GS PR 36E 2400 CHILLER GS PR 36F 1200 GS PR 36F 1200 CHILLER GS PR 36F 2400 GS PR 36F 2400 CHILLER GS Run 12A 1200 GS Run 12A 1200 CHILLER GS Run 12A 2400 GS Run 12A 2400 CHILLER GS Run 12C 1200 GS Run 12C 1200 CHILLER GS Run 12C 2400 GS Run 12C 2400 CHILLER GS Run 12D 1200 GS Run 12D 1200 CHILLER GS Run 12D 2400 GS Run 12D 2400 CHILLER GS Run 12F 1200 GS Run 12F 1200 CHILLER GS Run 12F 2400 GS Run 12F 2400 CHILLER GS Run 36A 1200 GS Run 36A 1200 CHILLER GS Run 36A 2400 GS Run 36A 2400 CHILLER Software and Firmware 3 3 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 3 4 Software and Firmware 377 96 and Chiller Module Filesa continued 377 96 Modules Chiller Modules GS Run 36C 1200 GS Run 36C 1200 CHILLER GS Run 36C 2400 GS Run 36C 2400 CHILLER GS Run 36D 1200 GS Run 36D 2400 GS Run 36D 2400 CHILLER GS Run 36F 1200 GS Run 36F 1200 CHILLER GS
15. RTANT If any wells leak flush the contaminated lanes then follow the table below If you are running Then Sequencing Electrophorese immediately then after each three loads GeneScan Leaking wells are not tolerated in GeneScan applications If a well leaks it is best to run another gel At the very least do not use the wells around the leaking lane Loading To load the gel Procedure Step Action 1 Press PAUSE during the prerun 2 Flush the wells with 1x TBE using a syringe Note Use care when flushing the wells Too much pressure could tear the wells and touching the teeth with the syringe could damage the comb and displace the teeth which could cause leakage Using the two pitch eight channel loader draw 2 uL of sample into the needles Clear any air gaps in the needles by dispensing 0 5 uL of sample or by dispensing until sample is visible in the tips of the syringe Using the comb markers as a guide align the needles into their respective lanes Very slowly dispense up to 1 5 uL of samples into the wells Load the odd lanes first Note For longer reads load the samples close to the gel surface rather than from the top of the well To accomplish this the two position loading syringe must have needles with 0 2 mm or 0 25 mm outer diameters With 0 3 mm outer diameter needles the samples must be gravity loaded After each loading rinse the needles with war
16. S OF at our full service in house repair center We also offer credit for buy backs and trade ins IN STOCK ITEMS www artisantg com WeBuyEquipment 7 EQUIPMENT DEMOS HUNDREDS OF Instra e REMOTE INSPECTION LOOKING FOR MORE INFORMATION MANUFACTURERS Remotely inspect equipment before purchasing with Visit us on the web at www artisantg com 7 for more our interactive website at www instraview com information on price quotations drivers technical LEASING MONTHLY specifications manuals and documentation RENTALS ITAR CERTIFIED CEP BAD tia Contact us 888 88 SOURCE sales artisantg com www artisantg com
17. Sequencer User s Manual Use 0 2 mm spacers P N 401837 and a 0 4 mm 96 lane casting comb P N 4305385 with the new stepped front plate P N 4305384 WARNING CHEMICAL HAZARD Acrylamide and Bis Acrylamide are both poisons neurotoxins irritant carcinogens and possible teratogens Acrylamide and Bis Acrylamide sublimes the solid releases toxic vapor and is harmful if swallowed inhaled or absorbed through the skin Effects are cumulative When handling always wear personal protection i e lab coat safety glasses and chemical resistant gloves and use in a well ventilated area Thoroughly clean surfaces subject to contamination i e binder clips combs and glass plates 2 Clamp the gels as shown below Use three stainless steel bulldog binder clips P N 4305386 on the top IMPORTANT To prevent well leakage the 96 lane plates and casting combs require 10 12 lbs clamping pressure End clamps align with front plate notch Clamping pressure on casting comb o o ac G IMPORTANT Be careful not to damage the teeth of the comb when attaching the clamps 2 2 Gels Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Preparing a Gel To prepare a gel for a run Step Action 1 Let the gel polymerize for at least two hours 2 Remove the clamps from the gel Leave the casting comb in place until you are ready
18. aranteed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Worldwide Sales Offices Applied Biosystems vast distribution and service network composed of highly trained support and applications personnel reaches into 150 countries on six continents For international office locations please call our local office or refer to our web site at www appliedbiosystems com Headquarters 850 Lincoln Centre Drive Foster City CA 94404 USA Phone 1 650 638 5800 Toll Free 1 800 345 5224 Fax 1 650 638 5884 Technical Support For technical support Toll Free 1 800 831 6844 ext 23 Fax 1 650 638 5891 www appliedbiosystems com u AS Applied sw Biosystems PE Corporation is committed to providing the world s leading technology and information for life scientists PE Corporation consists of the Applied Biosystems and Celera Genomics businesses Printed in the USA 09 2000 Part Number 4305423B Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com A rtisan Artisan Technology Group is your source for quality Technology Group new and certified used pre owned equipment FAST SHIPPING AND SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT DELIVERY Experienced engineers and technicians on staff Sell your excess underutilized and idle used equipment TENS OF THOUSAND
19. c Glen Waverly Victoria 3150 ihdo esia Australia Australia Malaysia Voice 61 0 3 9887 6262 j Fax 61 0 3 9887 9585 New Guinea E mail wpiau c031 aone net au New Zealand B 2 Two Pitch Eight Channel Loader Suppliers Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Supplier Supplier Contact Geographic Areas Served World Precision Liegnitzer Str 15 Y Austria eee Inc D 10999 Berlin Germany Bulgaria Voice 49 0 30 6188845 Czechoslovakia Fax 49 0 30 6188670 E mail wpi wpi sireco de Germany Greece Holland Netherlands Hungary 4 Italy Poland Rumania Russia Switzerland Yugoslavia World Precision 1 4 2 702 Naka Meguro Meguro Japan Instruments Inc Tokyo 153 0061 Japan Japan Voice 81 0 3 3760 5050 Fax 81 0 3 3760 5055 E mail wpi tkb att ne jp World Precision Astonbury Farm Business Centre Belgium Instruments Inc Aston Stevenage United Kingdom Hertfordshire SG2 7EG England Jemmen England Voice 44 0 1438 880025 Fax 44 0 1438 880026 Finland E mail wpi piuk demon co uk France Ireland Norway 4 Portugal Scotland Spain Sweden Two Pitch Eight Channel Loader Suppliers B 3 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Supplier Supplier Contact Geographic Areas Served World Preci
20. chloride Also includes GeneScan 350 Internal Lane Size Standard and loading buffer 402247 Kit B Amplified PCR products Contains four tubes of pooled combined PCR products To generate the products each DNA sample CEPH 1347 01 1347 02 1347 10 1347 15 has been amplified with the same six fluorescent labeled PCR primer pairs in kit A All of the PCR products from one tube can be detected in one gel lane Part Numbers C 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ABI PRISM Linkage Mapping 50 Rxn Kits 300 Rxn Kits Panel Chromosome Set Version 2 403089 403118 Complete Set 1 22 X 403090 403119 1 1 403091 403120 2 1 403092 403121 3 2 403093 403122 4 2 403094 403123 5 3 4 403095 403124 6 3 4 403096 403125 7 3 4 403097 403126 8 5 6 403998 403127 9 5 6 403099 403128 10 5 6 403100 403129 11 7 8 403101 403130 12 7 8 403102 403131 13 9 10 11 403103 403132 14 9 10 11 403104 403133 15 9 10 11 403105 403134 16 9 10 11 403106 403135 17 12 13 403107 403136 18 12 13 403108 403137 19 12 13 403109 403138 20 14 403110 403139 21 15 16 403111 403140 22 15 16 403112 403141 23 17 18 403113 403142 24 17 18 403114 403143 25 19 20 21 22 403115 403144 26 19 20 21 22 403116 403145 27 19 20 21 22 403117 403146 28 X C 6 Part Numbers Artisan Technology Gro
21. dules that corresponds to your desired filter set from the appropriate pulldown menus c Select the proper instrument file matrix for your run IMPORTANT The tracker will not function unless the matrix file was selected before starting the run d Select the proper sample sheet 5 Perform the plate check prerun and run procedures as instructed in the ABI Prism 377 DNA Sequencer User s Manual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Loading Gels Loader Options The following loaders can be used to load a 96 lane gel Fixed pitch 10 8 mm loader and a plate rack holding micro amp tubes spaced 10 8 mm apart P 10 microliter pipet with a flat loading tip Single barrel syringe with 0 2 mm or 0 3 mm needles Two pitch eight channel loader Note For reasons of loading speed and accuracy Applied Biosystems highly recommends using a two pitched eight channel loader to load 96 lane gels Two Pitch Eight The following schematics depict generic two pitched eight channel Channel Loader loaders in their closed position Note For specific vendor information see Two Pitch Eight Channel Loader Suppliers on page B 1 00000000 Oo 000 0 0 0 0 DTU O Or 0 0 00 00000000
22. eScan run modules and sample sheets are maintained in the Preference file CCD Pixel Position The instrument is shipped with the correct CCD pixel position value in memory When a run is started the software checks for a value greater than zero If the value is lost from memory an error message is displayed at the beginning of the run as shown below Could not complete your request because the CCD pixel position is incorrect The CCD pixel position value may become corrupted as the result of a power surge or power failure If this occurs you must enter the correct value before starting the run For details refer to the ABI PRISM 377 DNA Sequencer User s Manual 3 2 Software and Firmware Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Module Files There are many choices available among the module files provided in the ABI PRISM 377 96 upgrade Because you may not use all of the module files move those you do not intend to use from the Modules folder into the Unused Modules folder 377 96 and Chiller Module Files 377 96 Modules Chiller Modules GS PR 12A 1200 GS PR 12A 1200 CHILLER GS PR 12A 2400 GS PR 12A 2400 CHILLER GS PR 12C 1200 GS PR 12C 1200 CHILLER GS PR 12C 2400 GS PR 12C 2400 CHILLER GS PR 12D 1200 GS PR 12D 1200 CHILLER GS PR 12D 2400 GS PR 12D 2400 CHILLER GS PR 12F 1200 GS PR 12F 1200 CHILLE
23. eet Use Chiller modules If no external cooling system is in place Try to start a run as follows a Click OK in the error message box and try to start the run b Open the Manual Control window and try to turn on the pump manually Call service Err Coolant Flow Failure Occurs after the pump was turned on and off three times to see if coolant flow was detected Open the Manual Control window and try to turn on the pump manually If the problem persists call service No flow detected Attempted Pump Restart Indicates the coolant pump was turned on but no coolant flow was detected by the flow switch Check the reservoir to see if there is liquid in the cooler Scanner Did Not Find lts Home Position Indicates the scanner did not find its home position prior to collecting data for a plate check prerun or run Reset by pressing the Reset button once on the back of the 377 Click the Resume button in the Collection Run window Troubleshooting 4 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Glass Plates Applied Biosystems does not support the use of third party plates or combs on the 377 instrument We have been manufacturing glass plates to exact tolerances for slab gel electrophoresis for over 10 years Our plates are highly refined Third party plates are not made to our proprietary process tolerances and
24. es 401969 Top pouring fixture 604014 Bottom pouring fixture 603873 Upper buffer chamber 603875 Lower buffer chamber 603822 Upper buffer electrode assembly 603823 Lower buffer electrode assembly 4303201 Front 36 cm well to read heat plate 4305386 Clamps glass 2 in Bulldog C 2 Part Numbers Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ABI PRISM DNA Fragment Analysis Kits and Reagents Internal Lane Size GeneScan 350 500 and 400HD contain enough material for 800 Standards lanes GeneScan 1000 and 2500 contain enough material for 400 lanes GeneScan 500XL contains enough material for 1600 lanes Loading buffer is included P N Item 401735 GeneScan 350 ROX 401736 GeneScan 350 TAMRA 402985 GeneScan 400HD ROX 401734 GeneScan 500 ROX 401733 GeneScan 500 TAMRA 403040 GeneScan 500XL TAMRA 403039 GeneScan 500XL ROX 401098 GeneScan 1000 ROX 401100 GeneScan 2500 ROX 401545 GeneScan 2500 TAMRA 401144 Loading buffer Fluorescent dNTPs For fluorescent labeling of DNA during PCR amplification P N Item Quantity 401894 IFIdUTP Set R110 R6G 3 3 and 12 nmol 8 x 30 uL and TAMRA 401896 R110 dUTP 6 nmol 2 x 30 uL 401897 R6G dUTP 6 nmol 2 x 30 uL 4018
25. g Starter Kit 4305026 HLA DRB Sequencing Based Typing Starter Kit 403085 MicroSeq 16S rRNA Gene Kit 4003015 Primer Island Transposition Kit Reagent Kit Protocols P N Protocol 402113 ABI Prism Dye Primer Cycle Sequencing Ready Reaction Kit Protocol 402114 ABI Prism Dye Primer Cycle Sequencing Core Kit Protocol 402078 ABI Prism Dye Terminator Cycle Sequencing Ready Reaction Kit Protocol 402116 ABI Prism Dye Terminator Cycle Sequencing Core Kit Protocol 403041 ABI Prism dRhodamine Terminator Cycle Sequencing Ready Reaction Kit Protocol 403057 ABI Prism BigDye Primer Cycle Sequencing Ready Reaction Kit Protocol 4303237 ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit Protocol Part Numbers C 11 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com User s Manuals 903433 ABI PRISM 377 DNA Sequencer User s Manual 902376 373 DNA Sequencing System User s Manual 904435 GeneScan Analysis Software User s Manual 902842 GeneScan 672 Software User s Manual 4303188 GeneScan Reference Guide Part Number Updates Part numbers are subject to change Consult the Applied Biosystems World Wide Web site www appliedbiosystems com techsupport for updated information C 12 Part Numbers Artisan Technology Group Quality Instrumentation Gu
26. ht of the samples due to extra scan width However the Neural Net Tracker has been trained using gels run according to the default parameters For example 48 lane gels run in XL mode and 36 lane gels run in Full Scan mode Any deviation from the default is likely to confuse the tracker resulting in mistracked lanes Setting Run To set gel run conditions Conditions Step Action 1 Open the 377 96 Collection software 2 Prepare a sample sheet as described in the ABI Prism 377 DNA Sequencer User s Manual IMPORTANT Preparing a sample sheet prior to the run is required for optimal tracker operation Gels 2 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Step Action 3 Select a new GeneScan or Sequencing run The Run window is displayed EC Run 3 26 98 5 47 PM ee Cem Plate Check Module _ Plate CheckE gt PreRun Module Run Module _ Seq Run 36E 1200 v Collect time hours Sample Sheet D Well to Read distance cm Instrument File Operator Lanes 96 y Run Mode 96 Lane Lane Sample Sample Name Sample File Name 4 Within the Run window perform the following a Select 96 from the Lanes pulldown menu Note The correct run mode is then automatically selected b Select the plate check Pre run and Run mo
27. ires a syringe with a 0 2 mm tip to facilitate loading at the bottom of the well Note For more details refer to the GeneScan Reference Guide and the LMS v 2 User s Manual 2 8 Gels Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Suggested Load The following schematic depicts a microtiter plate showing the Mapping suggested load mapping for an eight channel loader Notes Loading in staggered format with a two pitch eight channel loader takes 12 loading steps Load No 1 12 4 The number in each well represents the respective gel lane position Odd lane loading positions are color coded on the comb 52 60 66 72 73 EA 20 Ge Load No 12 2 POLO 9 EEE Load No 11 H 90000000 LoadNo10 1100606006 Load No 9 OOG ME B MH Load No 8 OOOO E E 4 Load No 7 53 69 68 1 77 a E Load No 6 Black 9 c 51 67 5 67 63 Load No 5 Blue 0606000606 LoadNo 4 Red 8 000 Load No 3 Black gt 3 o 15 E E amp Load No 2 Blue 1 O 3 o En 15 Load No 1 Red lt o a a a o Gel lane position Gels 2 9 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Suggested Loading Follow the loading procedure to load the odd lanes first electrophorese Sequence for 2 min then load the even lanes IMPO
28. m dH O and blot dry with a lint free tissue to remove residual salt and prevent clogging Continue to load until all odd lanes have been loaded 2 10 Gels Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Step Action 9 Electrophorese for 2 min 10 Repeat steps 2 7 to load the even lanes 11 End the prerun and begin the run Gels 2 11 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 2 12 Gels Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Software and Firmware Overview In This Chapter The following topics are covered in this chapter Topic See Page Software 3 2 Firmware 3 5 Software and Firmware 3 1 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Software GeneScan Analysis The current GeneScan Analysis software is v 3 0 with GSGelTracker Sequence Analysis The current Sequence Analysis software is v 3 2 with SAGelTracker Collection The current Collection software for 96 lanes is v 2 5 Software Preference File Define default values in the new software following the procedures described under Setting Preferences in the AB Prism 377 DNA Sequencer User s Manual The default values you define for Sequencing or Gen
29. may exhibit variances from the necessary dimensions 46 Troubleshooting Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Filter Set Dye Combinations Virtual Filter Set Dyes Chemistry A GeneScan R110 R6G TAMRA ROX F dNTP Sequencing JOE A 5 FAM C TAMRA Dye primer G ROX T REG A ROX T R110 G Dye terminator TAMRA C C GeneScan 6 FAM TET HEX TAMRA Linkage Mapping Set V 1 Sequencing None None D GeneScan 6 FAM HEX NED ROX Linkage Mapping Set V 2 Sequencing None None E GeneScan None None Sequencing dR6G dTAMRA dR110 dROX BigDve Terminator Y BigDye Primer dRhodamine terminator F GeneScan 5 FAM JOE NED ROX AmprF STR Profiler PCR Amplification Kit AmpEsSTR Profiler Plus PCR Amplification Kit Plus PCR Amplification Kit AFLP Plant Mapping Kit Sequencing None None Filter Set Dye Combinations A 1 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com A 2 Filter Set Dye Combinations Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Two Pitch Eight Channel Loader Suppliers Supplier Information Tables Introduction For your convenience the following tables provide information on suppliers of two pitch eight channel loaders IMPORTANT
30. mb The 96 lane plates and casting combs require 10 12 lbs clamping pressure To prevent well leakage only use clamps that meet this requirement Use our stainless steel bulldog clips P N 4305386 or measure other clamps with a force gauge There are known conflicts with some of the Mac OS 8 extensions It is important to turn off these extensions before beginning any 96 lane run From the Extensions Manager window turn off the following extensions Open Tpt Modem Open Tpt Remote Access Open Tpt Serial Arbitrator Can Run on Any Macintosh Computer 96 lanes can be run on any Macintosh computer supplied with the 377 instrument Processor Speed The processor speed does not impact Collection but it does impact the speed of analysis Hard Drive Disk Space The hard drive must have enough disk space to hold a 70 MB gel file A CD Drive Is Required to Load Analysis Software Analysis will work on any Macintosh computer However the 7100 Macintosh computers supplied with the 377 instrument were not ship ped with a CD drive which is required to load the Analysis software Introduction l 3 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Kit Configurations Kit Configurations The following table lists the components and quantity of components Table included in ABI PRisM 377 DNA Sequencer 96 lane upgrade kits Component Quantity Part No P
31. ndent the surface of the gel If the surface of the gel is not completely flat in the loading region insert some of the teeth below the surface of the gel up to 0 5 mm so that all of the teeth touch the gel surface e lfatooth has penetrated the gel surface do not attempt to withdraw the comb This will cause sample to leak into adjacent wells 10 Place the gel and the cassette in the 377 instrument Note For instructions on setting up the 377 instrument for a run refer to the ABI PRISM 377 DNA Sequencer User s Manual 2 4 Gels Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Setting Run Conditions Selecting a Run Use the following table to select the type of comb to use based on the Mode number of lanes you are running Note The correct run mode is automatically chosen when the number of lanes is selected No of Lanes Comb Run Mode 24 Shark s tooth Full Scan 24 Square tooth Full Scan 32 Shark s tooth Full Scan 34 Square tooth Full Scan 36 Shark s tooth Full Scan 36 Square tooth Full Scan 48 Shark s tooth XL Scan 50 Square tooth XL Scan 64 Shark s tooth XL Scan 66 Square tooth XL Scan 96 Shark s tooth 96 Scan Note It is possible to run a gel of any number lanes in 96 Scan mode There will be the same number of data collection points per lane but there will be an area of blank space to the left and rig
32. ntinue to use the instrument as usual Problem Possible Cause Solution Odd and even lanes overlap Running too long between staggered loadings Shorten the run time between loadings Too much salt in the sample Resuspend samples in formamide only Perform extra 70 ethanol rinse of samples if precipitated may lead to slight loss in signal Signal showing up in neighboring lanes Leaky lanes Check clamps and comb fit Signal intensity very high and signal is being detected in neighboring lanes due to closeness of spacing Move tracker lane position from center of band to the edge of the band away from the strong signal and extract as usual Use one or two lane averaging to extract lanes Load less volume Troubleshooting 4 3 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Problem Possible Cause Solution Signal too weak Multiple Increase the CCD gain to four a Open 377 96 Collection b Inthe Run window select the Run module c Double click the small document icon next to the Run Module pulldown menu d Change the CCD gain to four Resuspend samples in less volume concentrate 377 96 Error Messages Message Possible Cause Solution A Valid 96 Lane Firmware Image is Required A non 96 collection software has tried t
33. o establish communications with a 377 instrument that has the 96 lane option installed Install the 96 lane collection software and firmware EP Voltage Deviation Exceeds Tolerance The EP voltage deviated outside ts tolerance range The instrument operation is paused Call service Warning Plate Out Thermistor P43 J43 Open Short Circuit Warning Plate In Thermistor P44 J44 Open Short Circuit Warning Possible Heater Thermistor Open Short Circuit Indicates one of the following Possible open or short circuit exists with the thermistor cable connected to J43 or J44 Temperature of the plate in an instrument with the 100k ohm thermistors is 21 9 C or less One of the thermistors is not functioning properly Schedule a service call and continue to operate the instrument as usual This message may appear when you launch data collection software and start a plate check prerun or run 44 Troubleshooting Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Message Possible Cause Solution Flow Detected With Pump Off External Cooling In Use Either The wrong module is being used for a run where an external cooling device is attached or The internal coolant system valve is stuck on or in the open position If an external cooling device is in use Check the modules selected on the run sh
34. sion Sarasota International Trade Center Areas not listed above Instruments Inc 175 Sarasota Center Blvd Otherworld wide Sarasota FL 34240 9258 areas USA Voice 941 371 1003 Fax 941 377 5428 E mail wpiOwpiinc com B 4 Two Pitch Eight Channel Loader Suppliers Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Part Numbers ABI PRISM 377 DNA Sequencer Parts Plates and Spacers P N Item 401878 48 cm Glass plates spacers kit includes two sets of 48 cm well to read glass plates and gel spacers 401876 36 cm Glass plates spacers kit includes two sets of 36 cm well to read glass plates and gel spacers 401877 12 cm Glass plates spacers kit Includes two sets of 12 cm well to read glass plates and gel spacers 401835 48 cm Rear glass plate 401838 48 cm Front glass plate 401837 48 cm Gel spacers 0 2 mm 2 401839 36 cm Rear glass plate 401840 36 cm Front glass plate 4305384 36 cm Front stepped plates 2 401833 12 cm Rear glass plate 401834 12 cm Front glass plate 4305384 36 cm Front stepped plates 2 Part Numbers C 1 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Cassette Buffer Chambers Heat P N Item Plate and Clamps 603627 Gel cassette 603947 Top and bottom gel pouring fixtur
35. ssure Burrs or bent teeth on comb Remove the burrs or replace the comb Bent kinked or Replace the spacers reset it to zero calibration scan damaged spacers Error Your The CCD Reset the CCD offset value CCD offset is reading is below 4 Open 377 96 Collection too high will zero during b In the Run window select the Run module c Double click the small document icon next to the Run Module pulldown menu d Change the CCD offset value to zero e Click Save as Default Comb is difficult to insert Using a different comb Be sure to use same comb for loading that was used for casting Clamps are too tight Insert comb slowly Fix any misaligned teeth with a syringe before they touch the gel Use looser clamps on future gels 42 Troubleshooting Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Thermistors Results Problem Possible Cause Solution Error Thermistor Failure One or more thermistors are bad Schedule a service call to replace the thermistors Continue to use the instrument as usual Error Temperature below thermistor limit Ambienttemperatureis too low lt 21 9 C for 100k thermistor Turn on the pump to warm the coolant to above 21 9 C Y Scedule a service call service to replace the thermistors Co
36. to insert the comb Rinse the plate thoroughly with dH O and let dry IMPORTANT Carefully clean the read region of the gel Slide out the casting comb without bending it IMPORTANT Do not pry the casting comb Using a razor blade scrape off all excess acrylamide from the glass in the loading area Using a squirt bottle rinse the loading area with dH20 and dry with at lint free tissue Using a syringe add 1x TBE in to the loading area Note Adding TBE eases the insertion of the comb IMPORTANT Be very careful not to introduce bubbles They are very difficult to remove once the comb has been inserted If necessary clean the shark s tooth comb with dH O and a lint free tissue Gels 2 3 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Step Action 9 Carefully insert the comb into the gel a Carefully align the center registration line on the comb with the registration mark on the back plate b Slide the comb between the plates IMPORTANT To avoid bending or breaking the teeth of the comb ensure all teeth enter the space between the plates at the same time Do not force the comb into the gel because the teeth will bend causing leaking c Continue to slide the comb down until the tips of the teeth just touch or slightly depress the surface of the gel i O d Teeth should just barely i
37. ty or increasing scan time Increased number of data collection to 480 channels allowing data collection of three channels plus two channel separation per lane Improved Neural Net Tracker decreasing labor to process gels Increased comb thickness in loading area to 0 4 mm while using 0 2 mm gel for electrophoresis causing no change in run time Improved comb durability and geometry allowing easier loading of volumes up to 1 5 uL Added position based CCD integration with time scaling allowing collection while accelerating the stage which minimizes noise Upgraded instruments still run 36 48 and 64 lane gels Hardware The following hardware is required to upgrade to 96 lane capability Required 4 ABI Prism 377 36 or 377XL DNA Sequencer Macintosh computer with the following specifications Power PC processor 32 MB RAM RAM modules supplied if required Mac OS 8 supplied in kit 1 2 Introduction Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Important Upgrade Notes Matrices Combs Clamps Mac OS 8 Extension Conflicts Computer Notes To ensure data quality we strongly recommend rerunning matrices at installation and semiannually for applications where matrices may be critical for optimal signal to noise ratio e g heterozygote detection and any GeneScan application The 96 lane run mode only supports the use of a shark s tooth co
38. up Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ABI PRISM Linkage Mapping P N Kit Quantity Set Version 2 450096 continued Individual Primer Pairs from the ABI PRISM Linkage Mapping Set Version 2 Must be ordered through Applied Biosystems Custom Oligonucleotide Synthesis Service specify locus name 3000 pmol 403061 True Allele PCR Premix 18 mL enough for 2000 rxns 403062 Control DNA CEPH 1347 02 180 uL enough for 150 rxns Part Numbers C 7 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ABI PRISM DNA Sequencing Kits and Reagents dRhodamine Terminator Cycle Sequencing Kits with AmpliTaq DNA Polymerase FS BigDye Primer Cycle Sequencing Ready Reaction Kits with AmpliTaq DNA Polymerase FS BigDye Terminator Cycle Sequencing Kits with AmpliTaq DNA Polymerase FS Dye Primer Cycle Sequencing Ready Reaction Kits with AmpliTaq DNA Polymerase FS C 8 Part Numbers Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com P N Kit Reactions 403044 Ready Reaction 100 403045 Ready Reaction 1000 4303143 Ready Reaction 5000 P N Primer Reactions 403051 21 M13 100 403049 21 M13 5000 403052 M13 Reverse 100 403050 M1
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