The Thermo Scientific KingFisher® Family
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1. best possible method for a sample process The Bindlt Software also enables the KingFisher Flex to interface with liquid handling robotics and plate stacking instruments providing a fully automated solution and the highest throughput is Bis OF eee FE ed te e te Ce a pripa tom bocd L a i ef a scat PL Dp piae saa Lii ah L Cine a ee Bon B FER melee L H a Saat Pacey KingFisher Flex 24 deep well magnet head and tip comb KingFisher Flex 24 deep well plate KingFisher Flex 96 deep well magnet head and tip comb Microtiter deep well 96 plate KingFisher Flex 96 KF plate KingFisher Flex 96 KF magnet head and tip comb KingFisher Flex 96 KF plate KingFisher Flex PCR magnet head and tip comb 96 PCR plate KingFisher Flex 96 KF plate KingFisher Flex magnetic heads with tip combs and and suitable microplates KingFisher provides a quick and easy solution to achieve high quality and reproducible results in the purification of nucleic acids proteins and cells with minimal hands on time It is an open and flexible system with a wide working volume range allowing the user to choose any available magnetic particle based purification kit suitable for the application With many applications available on the KingFisher website www thermo com kingfisher it is easy to create a new protocol for a specific application with the Bindlt Software Magnetic particl2. 0 pcs Ss i k SCIENTIFIC
3. Collection efficiency 295 295 299 43 1 801 40 0 of the particles Belgium Magnetic rods 96 or 24 15 24 32 2 482 30 30 Plate types 24 deep well plate 200 5000 ul 96 deep Special tube strip 1 x 5 tubes 1000 Special microstrips 1 x 8 wells 8 x 12 grid F well plate 50 1000 KingFisher 96 plate tube format 100 amp 200 pl well volumes ee 20 250 pl PCR plate 20 100 pl 33 2 2803 2000 Tip combs Special design 96 or 24 format Special design 1 x 5 format Special design 1 x 12 format Germany national toll free Heating temperature Heating block eae from room Ambient no heating Ambient no heating 08001 536 376 temperature to 115 Magnetic particle a i ee ca gt 1 um ca gt 1 um Germany international size 49 6184 90 6940 Keyboard Display START STOP PAUSE OK four arrow keys START STOP two cursor keys LCD START STOP two cursor keys LCD Italy two keys to rotate the turntable 39 02 02 95059 434 254 Internal Software Space for ca 100 internal protocols Space for ca 8 internal protocols Space for ca 8 internal protocols Bindlt Software yes yes yes Netherlands compatibility 31 76 571 4440 Computer interface USB or serial RS 232C port Serial RS 232C port Serial RS 232C port Nordic countries Robot compatibility Yes No No 358 9 329 100 Size WXDXH mm ca 680 x 600 x 380 290 X 290 X 310 290 X 290 X 310 Russia CIS aee a it uu 7 812 703 42 15 Bindit Software specifications i Supported operating Microsoft Windows XP Pro
4. The Thermo Scientific KingFisher Family Part of Thermo Fisher Scientific SCIENTIFIC The Thermo Scientific KingFisher purification system offers you e Fully automated high speed purification e High throughput 96 sample processing e Purification of any sample from any source e Pre defined and custom protocols e Easy operation via instrument or external PC The KingFisher family comprises of three systems KingFisher KingFisher mL and KingFisher Flex covering working volumes from 20 5000 ul Each system consists of an instrument specially designed plastics and easy to use software With optimized protocols you can be up and running in a matter of minutes e KingFisher 20 200 ul in microstrips up to 24 samples per run e KingFisher mL 50 1000 ul in tube strips up to 15 samples per run e KingFisher Flex 20 5000 ul in 96 and 24 deep well 96 KF and 96 PCR plates up to 96 samples per run Solve those purification problems Speed and sample purity continue to be a major concern to genomics and proteomics researchers The sheer volume of work involved in genome and protein mapping means processing speed is essential Likewise unsatisfactory results on even the most sophisticated analysers are increasingly linked to poor initial sample quality However the variety of samples being used from rare cell types and mRNA transcripts to increasingly large and delicate protein complexes presents a rea
5. e based kits offer excellent performance for the purification of DNA and RNA with KingFisher KingFisher mL or KingFisher Flex The range of starting material for nucleic acid research is broad from 1ml of blood for routine testing to very small samples e g from forensic surface and contact swabs hair and cigarette butts KingFisher enables you to isolate DNA or RNA in sufficient quantities from all kinds of material sources such as serum blood bacteria cell culture tissue soil or faeces Plant kits can be used to purify DNA from several plant species and sources e g leafs and seeds Using magnetic bead based separation technology ensures the resulting DNA and RNA is free of protein nucleases and other contaminants ensuring your purified nucleic acid samples are ready to use in downstream applications With KingFisher instruments it is possible to save time and minimise manual handling steps including pipetting centrifugation and mixing Automation of the purification process and elimination of any liquid handling steps result in reliable and robust operation Lambda Hind Ill KingFisher can be used to purify all kinds of proteins from recombinant to therapeutic antibodies The magnetic separation technology solves the problem of purifying recombinant proteins with highly complex structures and varying physiochemical properties The KingFisher technology and range of chromatography beads enable routine purification of antibodies ant
6. fessional with SP2 or later and Vista Internet Explorer 6 0 or later must be installed Spain Portugal system 34 93 223 09 18 Used to create modify and run the protocols with KingFisher instruments Switzerland 41 44 454 12 12 eee UK Ireland Thermo Scientific KingFisher Ordering Information 44 870 609 9203 5400000 KingFisher Magnetic Particle Processor 100 240 V 50 60 Hz for microplates Asia 5400050 KingFisher mL Magnetic Particle Processor 100 240 V 50 60 Hz China 5400610 KingFisher Flex Magnetic Particle Processor with 96 PCR head 100 240V 50 60 Hz 86 21 6865 4588 or 5400620 KingFisher Flex Magnetic Particle Processor with KF head 100 240V 50 60 Hz 86 10 8419 3588 5400630 KingFisher Flex Magnetic Particle Processor with 96 deep well head 100 240V 50 60 Hz a 5400640 KingFisher Flex Magnetic Particle Processor with 24 deep well head 100 240V 50 60 Hz India Software 91 22 6716 2200 5189010 Bindlt Software CD and user manual Japan Accessories and spare parts 481 45 453 9220 24074411 KingFisher Flex 96 PCR head and heating block l F 24074421 KingFisher Flex 96 KF head and heating block Other Asian countries 24074431 KingFisher Flex 96 deep well head and heating block 852 2885 4613 24074441 KingFisher Flex 24 deep well head and heating block Countries not listed 11273100 KingFisher mL tube tray 49 6184 90 6940 or 24073180 KingFisher mL microtube tray 33 2 2803 2000 24074410 KingFisher Flex 96 PCR head 24074420 Ki
7. igens pharmaceuticals and vaccines using any kind of host cell mammalian insect yeast bacteria or transgenic animals plants KingFisher can also significantly improve the phage display process by fully automating the cycles of the biopanning phase The Bindlt Software allows you to develop your own robust protocols for other protein applications like glycoprotein purification protein arrays protein interaction or protein phosphorylation studies _ Hands on KingFisher Flex 24 Hands free time Spin Column min 0 00 0 30 1 00 1 30 2 00 2 30 Figure 1 The time used for purification of DNA from 1ml of blood The comparison of isolating 24 samples between KingFisher Flex and a column based isolation method _ O a a na ua ua aay ay ua AF ae a Py at hr ty i Lambda Hind Ill Figure 2 The quality and quantity of genomic DNA isolated from blood with KingFisher Flex is excellent BIND sample with beads WASH Figure 3 Rapid and robust sample preparation to get rid of impurities purified product North America Technical Specifications USA Canada 1 866 984 3766 Thermo Scientific KingFisher Flex Thermo Scientific KingFisher mL Thermo Scientific KingFisher Processing volume 96 20 1000 ul 50 1000 pl 20 200 ul Europe 24 200 5000 ul Austra Capacity samples run 96 or 24 15 24
8. l challenge On all counts KingFisher automated purification systems ensure impurities are removed DNAIRASA Cell Protein Other Select START Run OK Details Work at unparalleled speed The processing speed of KingFisher systems is unparalleled and stems from their advanced magnetic particle processing technology which uses magnetic rods to move sample particles through the various purification phases binding mixing washing and incubation eliminating liquid handling and increasing automation results in a reliable and robust technique The overall efficiency brings speed for true market leading throughput The top of the range KingFisher Flex can purify 96 samples in just 15 30 minutes Equally Important sample cross contamination and reagent cross over is effectively eliminated by the automated purification process Adapt to your own routine KingFisher enables you to increase your workflow flexibility Pre defined application protocols are available for many existing magnetic particle based methods Additionally it is easy to customise existing protocols or create entirely new ones using the intuitive Thermo Scientific Bindlt software Protocols can then be run either directly via the KingFisher instrument or remotely via a PC See instant benefits in productivity Installing a KingFisher system will make an instant impact on your work You will be able to purify samples at speeds you never believed possible
9. ngFisher Flex 96 KF head 24074430 KingFisher Flex 96 deep well head 24074440 KingFisher Flex 24 deep well head www thermo com 24075410 KF Flex 96 PCR heating block kingfisher 24075420 KF Flex 96 KF heating block 24075430 KF Flex 96 deep well heating block 24075440 KF Flex 24 deep well heating block Consumables for KingFisher 97002070 KingFisher tip comb 50 pcs 97002080 KingFisher plate 100 ul 50 pcs 97002084 KingFisher plate 200 ul 50 pcs 97002090 KingFisher plastics 100 ul 8 pack 8 plates 100 pl 8 tip combs box 97002094 KingFisher plastics 200 ul 8 pack 8 plates 200 pl 8 tip combs box Consumables for KingFisher mL 97002111 KingFisher mL tip comb 800 pcs 97002121 KingFisher mL tube 900 pcs 20x45 pcs 97002131 KingFisher mL Combi 60 tubes and tip combs for 60 samples 97002141 KingFisher mL Combi 240 tubes and tip combs for 240 samples Consumables for KingFisher Flex 97002514 KingFisher Flex 96 tip comb for PCR magnets 80 pcs 97002524 KingFisher Flex 96 tip comb for KF magnets 100 pcs 97002534 KingFisher Flex 96 tip comb for deep well magnets 100 pcs 97002610 KingFisher Flex 24 deep well tip comb and plate 50 pcs of each 97002540 KingFisher Flex 96 KF plate 200 48 pcs 95040450 Microtiter deep well 96 plate V bottom polypropylene 50 pcs 95040460 Microtiter deep well 96 plate V bottom sterile polypropylene 50 pcs 95040470 KingFisher Flex 24 deep well plate 50 pcs 95040480 KingFisher Flex 24 deep ct plate sterile 5
10. tics are ideal for magnetic particle processing due to their low binding affinity for biomolecules KingFisher mL processes samples in tube strips whilst KingFisher and KingFisher Flex utilise specially designed microplates The magnets are protected by disposable tip combs The design of these specialist plastics enables excellent recovery of magnetic particles Thermo Scientific Microtiter deep well plates King Fisher 96 KF plates and KingFisher Flex 24 deep well plates are all specially designed for KingFisher Flex and enable efficient processing and mixing of magnetic particles Both the yield and quality of the isolated DNA RNA is significantly improved with special KingFisher plates In addition fully skirted rigid PCR plates can be used with KingFisher Flex e High resistance to organic solvents e High temperature resistance 196 to 121 C e Sealable with adhesive films e 96 deep well plate sealable with cap mat Thermo Scientific Bindit Software the perfect complement to a peerless system Bindlt Software is designed to allow you to develop custom made protocols or modify existing protocols for your own applications allowing increased flexibility Additionally Bindlt Software enables you to set user control access levels advanced protocol management sample information and clear reporting Pre defined and validated purification protocols are available for different applications offering the possibility to choose the
11. with minimal hands on requirement as the automation takes over KingFisher will leave you free to concentrate on more involved downstream analysis The Thermo Scientific KingFisher family Thermo Scientific KingFisher Flex Thermo Scientific KingFisher Using either a 96 or 24 plate format KingFisher Flex provides the fastest and easiest way to purify genomic DNA or RNA and proteins from a variety of sample material Supplied with predefined protocols KingFisher Flex also allows you to create your custom made protocols for special applications Samples run 96 or 24 Working KingFisher gives you the ability to purity small scale samples All purification and processing steps are carried out In microplates with simple push button operation Samples run Working volume in pl volume in pl 24 format 200 5000 96 format 20 1000 Thermo Scientific KingFisher mL KingFisher mL is your choice for high volumes It allows all purification and processing steps to be carried out in a single strip of five tubes Being able to go as low as 50 ul to release target molecules enables cells and proteins from larger starting volumes to be isolated and concentrated simultaneously Samples run 15 volume in pl Thermo Scientific KingFisher Plastics KingFisher instruments utilise disposable plastics made of polypropylene and they are specially designed for the processing and isolation of target molecules The plas
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