QIAgility™ — Pure Precision
Contents
1. QlAgility Pure Precision 06 2010 Sample amp Assay Technologies WWW diagen com Australia Orders 1 800 243 800 Fax 03 9840 9888 Technical 1 800 243 066 Austria Orders 0800 28 10 10 Fax 0800 28 10 19 Technical 0800 28 10 11 Belgium Orders 0800 79612 Fax 0800 79611 Technical 0800 79556 Brazil Orders 0800 557779 Fax 55 11 5079 4001 Technical 0800 557779 Canada Orders 800 572 9613 Fax 800 713 5951 Technical 800 DNA PREP 800 362 7737 China Orders 86 2 1 3865 3865 Fax 86 21 3865 3965 Technical 800 988 0325 Denmark Orders 80 885945 Fax 80 885944 Technical 80 885942 Finland Orders 0800 914416 Fax 0800 914415 Technical 0800 914413 France Orders 01 60 920 920 Fax 01 60 920 925 Technical 01 60 920 930 Germany Orders 02103 29 12000 Fax 02103 29 22000 Technical 02103 29 12400 Hong Kong Orders 800 933 965 Fax 800 930 439 Technical 800 930 425 Ireland Orders 1800 555 049 Fax 1800 555 048 Technical 1800 555 061 Italy Orders 800 789 544 Fax 02 334304 826 Technical 800 787980 Japan Telephone 03 6890 7300 Fax 03 5547 0818 Technical 03 6890 7300 Korea South Orders 080 000 7146 Fax 02 2626 5703 Technical 080 000 7145 Luxembourg Orders 8002 2076 Fax 8002 2073 Technical 8002 2067 Mexico Orders 01 800 7742 639 Fax 01 800 1 122 330 Technical 01 800 7742 436 The Netherlands Orders 0800 0229592 Fax 0800 0229593 Technical 0800 0229602 Norway Orders2. from the QIAgility 10 Graduated FlatBase Tubes for use with the QIAgility 50 x 5 ml Reagent Trough Lid for use with the QIAgility 10 x 70 ml Reagent Trough Lid for use with the QIAgility 20 x 170 ml Reagent Trough Lid for use with the GlAgility 15 x 270 ml Cat no 2001531 2001532 Inquire Inquire PAGS 2 290577 220530 2709937 990554 990556 990558 www qiagen com GlAgility Pure Precision 06 2010 For up to date licensing information and product specific disclaimers see the respective QIAGEN kit handbook or user manual QIAGEN kit handbooks and user manuals are available at www giagen com or can be requested from QIAGEN Technical Services or your local distributor Purchase of this product Rotor Gene QuantiFast and QuantiTect SYBR Green Kits is accompanied by a limited non transferable immunity from suit to use it with detection by a dsDNA binding dye as described in U S Patents Nos 5 994 056 and 6 171 785 and corresponding patent claims outside the United States for the purchaser s own internal research No real time apparatus or system patent rights or any other patent rights and no right to use this product for any other purpose are conveyed expressly by implication or by estoppel This product Rotor Gene QuantiFast and QuantiTect Probe and Multiplex Kits and Type it Probe Kits is an Authorized 5 Nuclease Core Kit without Licensed Probe Its purchase price includes a li
3. 800 18859 Fax 800 18817 Technical 800 18712 Singapore Orders 1800 742 4362 Fax 65 6854 8184 Technical 1800 742 4368 Spain Orders 91 630 7050 Fax 91 630 5145 Technical 91 630 7050 Sweden Orders 020 790282 Fax 020 790582 Technical 020 798328 Switzerland Orders 055 254 22 11 Fax 055 254 22 13 Technical 055 254 22 12 UK Orders 01293 422 911 Fax 01293 422 922 Technical 01293 422 999 USA Orders 800 426 8157 Fax 800 718 2056 Technical 800 DNA PREP 800 362 7737 QIAGEN Dm Sample amp Assay Technologies
4. GlAgility Pure Precision 09 TT QIAGEN Sample amp Assay Technologies Fully automated sample and assay technologies The QlAgility is a compact benchtop instrument that enables automated PCR setup in a wide range of formats including PCR assays for the Rotor Gene Q The high precision of the QlAgility delivers the reproducible results you need in your end point and real time PCR assays from sample to sample and laboratory to laboratory Automated PCR setup is rapid and reliable and eliminates manual pipetting steps that can be prone to human error The unmatched versatility of the QIAgility means that almost all tube and plate formats are supported as well as Rotor Discs for the Rotor Gene Q The QIAgility provides E Automated PCR setup in all formats M Convenient easy to use software E Standardized results and increased productivity M Elimination of manual pipetting steps E Seamless integration with QIAGEN sample technologies The GlAgility is operated via a computer with easy to use software Simply select a program set up the worktable and walk away during PCR setup Samples are ready for immediate use in downstream applications QlAgility www qiagen com GlAgility Pure Precision 06 2010 Rapid high precision PCR setup that you can rely on Advanced technology for pure precision Dynamic liquid level sensing enables the GlAgility to deliver high precision standardized
5. al B GlAgility M Prp HugL Cyst Prp HugL Cyst 0 4 s x z s o 2 z 2 x 4 q x 4 Y 1 t 1 S 0 3 O DOO 00 s LOU O 2000 D E 0 2 Ten a Y Pa 5 Der em mea 0 So 0 1 400 00 5 10 15 20 25 30 35 40 45 wo 0 oo Cycle Figure 3 Automated reaction setup is more accurate than F i manual reaction setup IL1 2RB1 was amplified from j l leukocyte RNA 1 10 and 100 ng using the Rotor Gene Q and the QuantiFast Probe RT PCR Kit Replicates of 3 were Figure 2 Eliminate manual pipetting steps Pro Hugl and Cyst were amplified from human set up EN manually and BI using the GlAgility DNA yields genomic DNA 10 ng PCR reactions were set up using the TopTaq Master Mix Kit in were quantified by quantitative real time RT PCR where the replicates of 2 using the QIAgility and manually After cycling reactions were analyzed by Rotor Gene Q was also used for analysis capillary electrophoresis using the QlAxcel www qiagen com GlAgility Pure Precision 06 2010 Flexible workflow with built in versatility The QlAgility is compatible with all your workflow needs The worktable is easy to set up and for optimal versatility is configured to support almost all PCR tube types and plate formats including 96 well and 384 well plates as well as Rotor Discs for the Rotor Gene Q The QlAgility can set up multiple master mixes and can process multiple PCR setups in parallel In addition to PCR setup the flexibility of the GlAgility allo
6. ip reuse No No No Yes No Yes 8 times No No Yes 8 times Yes 8 times Yes 8 times Yes 8 times Yes 8 times Tip consumption 108 x T 2a x 154x T 20x IRS 24 12x voor 11 xT 5 x To 10 x T LOS xT 110 x T 10 x T LOS ba 115 x T 5x T Run time minutes 28 17 71 46 F T 50 pl Tips 1 T 200 pl Tips The GlAgility can be used in a wide range of liquid handling operations The user can control tip usage enabling cost efficient use of consumables Reactions can be set up using master mix that has already been prepared or the QIAgility can prepare the master mix QlAgility Pure Precision 06 2010 www qiagen com TEC Babi 46666660 6 8 8 ian Ti em Mum ma esta Em TI LA TIT HELL IM Xj eM umm m mam rss de ee GlAgility ss Intuitive software for ease of use With the QIAgility specialist training is not required QlAgility software simulates the instrument worktable making it exceptionally easy to use by the automation novice or more experienced laboratory personnel Fast setup and development of user protocols are facilitated by wizards which guide the user through software procedures in a stepwise fashion The QlAgility is preinstalled with optimized protocols for convenient and effective PCR setup The software automatically calculates reagent volumes for the selected numbe
7. mited non transferable immunity from suit under certain patents owned by Roche Molecular Systems Inc or F Hoffmann La Roche Ltd for using only this amount of the product in the practice of the 5 nuclease process solely for the purchaser s own internal research when used in conjunction with Licensed Probe No right under any other patent claims such as apparatus or system claims and no right to use this product for any other purpose is hereby granted expressly by implication or by estoppel Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA Purchase of this product Rotor Gene QuantiFast and QuantiTect RT PCR Kits is accompanied by a limited non transferable license under RT and Reverse Transcription PCR patents owned by Roche Molecular Systems Inc and F Hoffmann La Roche Ltd to use it for the purchaser s own internal research No real time patent rights of any kind no right under any other patent claims such as apparatus or system claims and no right to use this product for any other purpose is hereby granted expressly by implication or by estoppel Purchase of this product QuantiTect Kits is accompanied by a limited license under U S Patent Numbers 5 035 996 5 945 313 6 518 026 and 6 287 823 and corresponding foreign patents The purchase of this product QuantiTect SYBR Green Kits includes a limited non
8. n Volume pl Mean mg S D mg CV 0 99 0 06 5 64 PAOS 0 05 220 5 02 0 03 0 56 9 99 0 02 0 24 19 97 0 04 0 22 49 99 0 05 0 10 100 84 0 14 0 14 150 79 0525 0 17 198 82 0 26 0 13 The QIAgility was used to pipet 1 2 5 10 20 50 100 150 and 200 pl water in replicates of 16 The actual volume pipetted was determined for all aliquots based on weight i e 1 g21000 pl The mean standard deviation S D and coefficient of variation CV was calculated for each volume GlAgility Pure Precision 06 2010 Sample amp Assay Technologies Reproducible results from sample to sample and laboratory to laboratory A owe no GeeS 6S 7 4249000 MEGET j B E Manual Absolute reliability and reproducibility 0 5 The QlAgility is provided with optimized protocols for unmatched ge reliability and reproducibility In the future more protocols will become o 03 available Manual pipetting steps which are typically prone to human E Ww m 5 02 error are eliminated Figure 2 and Figure 3 Using the software in Virtual So 9 Mode enables user developed protocols to be tested in the absence of reagents avoiding costly repetition of experiments To reduce your work 0 0 i 5 10 15 20 25 30 35 40 45 load there is no need for time consuming manual documentation of runs Cycle i since comprehensive run reports containing all run information are generated before and after each protocol QlAgility Manu
9. ooocoseees ooo oo bese eee ooooooeeeeses COCO eee i E E CE Reaction Ml tube QIAGEN 75 BiERBI szsssisEs pn HELE EEE BS3RERRRARNNDENIN BREERARSRRERESRS Ordering Information Product QIAgility System incl PC QIAgility System HEPA UV incl PC Rotor Disc 100 Starter Kit Rotor Disc 72 Starter Kit 50 pl Conductive Filtered Tips 960 200 pl Conductive Filtered Tips 960 Tip Receptacle Box 10 5 ml Tube Graduated Flat Base 50 Reagent Trough Lid 70 ml 10 Reagent Trough Lid 170 ml 20 Reagent Trough Lid 270 ml 15 Contents Robotic workstation for automated PCR setup without UV light and HEPA filter 1 year warranty on parts and labor installation and training included Robotic workstation for automated PCR setup with UV light and HEPA filter 1 year warranty on parts and labor installation and training included Kit includes 2 Rotor Disc 100 packs Rotor Disc Heat Sealer Rotor Disc Heat Sealing Film Rotor Disc 100 Rotor and Locking Ring Rotor Disc 100 Loading Block Rotor Disc Pipetting Aid Kit includes 3 Rotor Disc 72 packs Rotor Disc Heat Sealer Rotor Disc Heat Sealing Film Rotor Disc 72 Rotor and Locking Ring Rotor Disc 72 Loading Block Rotor Disc Pipetting Aid Conductive Filtered Tips for use with the GlAgility 960 x 50 pl Conductive Filtered Tips for use with the GlAgility 960 x 200 yl Tip Receptacle Boxes for disposal of used tips
10. oppel for any reagent or kit or under any other patent or patent claims owned by the University of Utah Research Foundation Idaho Technology Inc Roche Diagnostics GmbH or by any other Party This product may be operated only with authorized reagents such as fully licensed QIAGEN kits and assays For further information on purchasing licenses for in vitro diagnostics reagents please contact Roche Molecular Systems 4300 Hacienda Drive Pleasanton CA 94588 and for non diagnostics reagents please contact Roche Diagnostics GmbH Sandhofer Strasse 116 68305 Manheim Germany For applicable countries This realtime thermal cycler is licensed under U S Patent No 6 814 934 and corresponding claims in any foreign counterpart patent thereof owned by Applied Biosystems LLC in all fields including research and development all applied fields and human and animal in vitro diagnostics No rights are conveyed expressly by implication or estoppel to any patents on real time methods including but not limited to 5 nuclease assays or to any patent claiming a reagent or kit For further information on purchasing additional rights contact the Director of Licensing at Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA Trademarks QIAGEN QIAgility QlAxcel cador EZ1 HotStarTaq QuantiFast QuantiTect TopTaq Type it Rotor Disc Rotor Gene Corbett Research 2009 2010 QIAGEN all rights reserved
11. pipetting Table 1 and Figure 1 The instrument senses the meniscus during each pipetting event and retracts the tip while dispensing enhancing accuracy and minimizing liquid carry over If the liquid is viscous the GlAgility can adapt its pipetting speed accordingly Exceptional safety features The advanced design of the GlAgility delivers improved process safety giving you peace of mind An in built sensor ensures that the instrument pauses when the hood is opened during a run protecting users from moving parts An optional UV light ensures effective worktable decontamination and an optional HEPA filter provides positive clean air pressure throughout PCR setup These safety features are complemented by intelligent safety warnings from the instrument software should an error occur 5 10 15 20 25 30 35 40 Cycle concentration Figure 1 Exceptional reproducibility and accurate series dilutions A 2 fold series dilution of human genomic DNA 30 ng to 0 6 ng was performed using the QlAgility ILTR2 was amplified from these dilutions using the Rotor Gene Q and the QuantiFast Probe PCR Kit Reactions were set up in replicates of 5 DNA yields were quantified by quantitative real time PCR where the Rotor Gene Q was also used for analysis Plots show EN highly precise and reproducible replicates with a 1 cycle difference between each 2 fold dilution and El the standard curve Table 1 Unrivaled precisio
12. r of samples giving you complete cost control In addition the user can develop custom protocols tailored to specific research requirements Comprehensive service and support At Tip 208i Conductive tip AGEN AZ Tip 50pl Comductive tip AGEN i ee Poe ent 1 323998 mig QIAGEN Instrument Service provides flexible service support agreements tailored to your needs We also provide Warranty PLUS extended warranties giving you complete cost control and enhanced coverage through priority response times Specifications of the QlAgility Dimensions Width 55 cm 21 6 in Depth 63 cm 24 8 in without cables Height 45 cm 17 7 in hood closed Weight 41 kg 90 2 lb standard configuration 100 240 V 250 VA 50 60 Hz 5 A fuse 1 96 samples per run Electrical requirements Sample throughput Warranty 1 year warranty on parts and labor Visit www giagen com goto QlAgility to find out more GlAgility Pure Precision 06 2010 Sample amp Assay Technologies ooo ooo ooo gog OVO cocco ooooooOoeeeeo caos Oca ooooooeteeteeo 000000006 ee Oo RR 0 oOoocooooo0ooo00 z Pi da d oe GO 8080000 eooooooooooo MI PPP Box e S R Pene ic tonno pite m B CE D wo HB x 000000000000 DODODDONDHODA 000000000000 oooooocoooooo0 oooooocoooooo0 ooooocooooooo 000000000000 000000000000 Tip tg Conductive Up GRADE VEDA ET mug ooo oo coe eee ew docce oocoooosesees ooo
13. setup system Real time PCR cycler Nucleic acid purification solution using magnetic particle technology QlAgility Pure Precision 06 2010 Sample amp Assay Technologies TI t ak Efficient walkaway automation The QIAgility enables rapid and efficient processing of complex reaction setups freeing up your valuable laboratory time Table 3 The single channel pipet can process up to 96 samples in just 17 minutes providing enormous time savings and eliminating tedious manual pipetting which can cause repetitive strain injury While the QIAgility is performing reaction setup the user can concentrate on more important laboratory tasks Table 3 Speed and versatility Application Normalization Reformatting Plate replication Plate replication Serial dilution Repetitive aliquoting Repetitive aliquoting Preparation of standards Master mix preparation PCR setup PCR setup PCR setup PCR setup Description One step dilution 1 1 transfer 1 4 transfer 1 4 transfer 16 concentrations Primer solution 2 ml into aliquots 20 pl Primer solution 2 ml into aliquots 20 pi 6 concentrations 8 components for 96 samples Premixed master mix Master mix preparation Premixed master mix and prediluted Master mix preparation and standard dilution Number of reactions 96 96 96 384 96 384 16 dilutions 96 aliquots 96 aliquots 6 dilutions 96 96 96 96 96 T
14. t PCR Kit Rotor Gene Kits QuantiFast Kits Quantilect Kits QIAGEN OneStep RT PCR Kit QuantiTect Kits QIAGEN OneStep RT PCR Kit Type it PCR Kits QIAGEN LongRange Kits HotStar HiFidelity Polymerase Kit cador veterinary PCR Kits HotStarTaq and HotStarTag Plus Kits QIAGEN Tag Kits QIAGEN Fast Cycling PCR Kit TopTag Kits www qiagen com Description Rapid and reliable RT PCR on the Rotor Gene Q Fast sensitive RT PCR on any real time cycler Sensitive RT PCR on any real time cycler Sensitive RT PCR with simple setup using any RNA template Highly sensitive detection of multiple viral DNA and RNA targets in the same tube Highly sensitive detection of viral RNA Accurate and reliable SNP genotyping mutation detection and microsatellite analysis Sensitive and accurate amplification of extremely long PCR products High fidelity hotstart PCR Ready to use validated real time PCR assays to detect veterinary pathogens Highly specific hot start PCR without optimization Highly reliable PCR at first attempt Rapid and specific PCR on any thermal cycler Unmatched reliability with unrivaled ease of use GlAgility Pure Precision 06 2010 Automated solutions from sample to result free up your time F Purification gt Assay setup gt Detection and analysis gt b V A i EZ1 Advanced 7 QlAgility Rotor Gene Q Real time PCR EZ1 Advanced XL PCR
15. transferable license under U S Patent No 5 871 908 and all continuations and divisionals and corresponding claims in patents and patent applications outside the United States owned by Roche Diagnostics GmbH for internal research use or for non in vitro diagnostics applications with authorized reagents with regard to Melting Curve Analysis No right is conveyed expressly by implication or estoppel under any other patent or patent claims owned by Roche Diagnostics GmbH or by any other Party Purchase of this product TopTaq DNA Polymerase is not accompanied by a license under patents owned by Roche Molecular Systems Inc and F Hoffmann La Roche Ltd No rights to realtime PCR or to any primers probes or pathogens are conveyed expressly by implication or by estoppel For applicable countries The purchase of this product includes a limited non transferable license to one or more of U S Patent Nos 6 787 338 7 238 321 7 081 226 6 174 670 6 245 514 6 569 627 6 303 305 6 503 720 5 871 908 6 691 041 7 387 887 7 273 749 7 160 998 U S Patent Application Nos 2003 0224434 and 2006 0019253 and PCT Patent Application No WO 2007 035806 and all continuations and divisionals and corresponding claims in patents and patent applications outside the United States owned by the University of Utah Research Foundation Idaho Technology Inc Evotec Biosystems GmbH and or Roche Diagnostics GmbH No right is conveyed expressly by implication or est
16. ws the user to program an unrivaled range of high precision pipetting applications which includes Normalization of DNA and RNA concentration Transfer of liquid samples from one tube format to another Restriction digest setup ES L Bi Serial dilutions with variable dilution ratios LJ E Sample pooling E Selective pipetting from archived sample banks GlAgility Pure Precision 06 2010 Sample amp Assay Technologies Tired of tedious manual pipetting steps Complete your fully automated workflow The GlAgility fully automates proven QIAGEN PCR kits Table 2 Visit www qiagen com for up to date product information For increased convenience the GlAgility provides a broad range of output formats enabling compatibility with all real time cyclers including the Rotor Gene Q When used in combination with the EZ1 Advanced automated solutions the GlAgility can complete and enhance your automated real time PCR workflow see next page Table 2 Use the GlAgility to fully automate proven QIAGEN PCR kits Application Gene expression analysis Virus detection Genotyping Cloning Pathogen detection Standard and specialized PCR applications Method Real time RT PCR Real time RT PCR Real time RT PCR End point RT PCR Real time PCR and RT PCR End point RT PCR End point PCR End point PCR and RT PCR End point PCR Real time PCR End point PCR End point PCR End point PCR End poin
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