G-STORM GS4M - G-Storm Thermal Cycler Systems
Contents
1. Manufacturer Art No amp Product Name Type BioPlastics B70511 96 Well thin wall plate 96 well non skirted B70661 96 Well thin wall plate 96 well semi skirted B70521 384 Well thin wall plate 384 well skirted Abgene AB 600 96 well non skirted AB 800 96 well skirted AB 900 96 well semi skirted AB 1111 384 well TF 0384 384 well Bilatec THERMOSPRINT 96 PP transparent 96 well non skirted 311296 only w o workstation THERMOSPRINT 384 PP transparent 384 well 310384 NUNC Art No 240600 96 well non skirted 96 Art No 230013 96 well semi skirted 96 Art No 230012 96 well skirted 96 Art No 230584 384 well 384 Eppendorf Art No 0030 128 648 twin tec plate 96 skirted Art No 0030 128 575 twin tec plate 96 semi skirted Art No 0030 128 508 twin tec plate 384 Corning Thermowell 96 Well Polypropylene Microplate Natural 6551 Thermowell 96 Well Polycarbonate Microplate Model M 6511 Corning 384 Well Polyproplyene Microplate 6500 Corning 384 Well Polyethylene Microplate 6502 56 Appendix G Storm Thermal Cyclers Order Information Part Description Thermal Cyclers GS00004M G STORM GS4 with Manual Lids Thermal Blocks GSB00096 96 well block GSFAST96 96 well Fast block GSB00384 384 well block GSFAST384 384 well Fast block GSOOSITU In situ block2. G Storm Thermal Cyclers Appendix 57 Sales amp Service G Storm Somerton Biotechnology Centre Ricksey Lane Catcombe Somerton Somerset TA11 7JH Phone 44 0 7919 121510 E Mail info gstormdirect co uk Visit our website www gstormdirect com 58 Appendix G Storm Thermal Cyclers Index AC Power Connection 16 Archiving Reports 47 Automatic Hot Start command 27 Barcode Input command 26 Block Exchange 52 Changing Users 37 Cleaning 48 Command buttons 19 Additional functions 20 Cycler Monitor 20 G Storm Logo 20 Incubate 19 Lightning flash 20 New Program 19 Program Wizard 19 Reports 20 Run Program 19 Settings 20 View Edit Program 19 Creating Programs 24 Cycler Installation 14 Cycler Management 21 Cycler Monitor 20 Cycler Settings 23 Cycler tests 15 Cycler validation Tests 15 Default Password 14 Deleting a Program Step 35 Deleting Programs 35 DND Programming 24 Editing Programs 34 End Cycle command 31 Exporting Program Files 46 Functional Overview 12 Fuses 16 Gradient Step command 30 Gradient Step Conversion 35 G Storm Thermal Cyclers Grounding Requirements 16 G Storm Logo button 20 Home Page 13 Importing and Exporting Files 46 Importing Program Files 46 Incubate temperature 36 Incubate button 19 incubation 36 Initial Inspection 10 Installation 8 Block Requirements 9 Electrical Requirements 8 Site Requirem
3. Ensure a plate is in the block for this test 1 Click Settings button 2 Click Browse cycler management 3 Click Cycler Tests 4 Highlight all cyclers in the top panel and click Engine Test When completed click OK Cycler Test procedures include specific instrument tests to ensure correct installation and operation of the thermal cycler After the tests have been completed successfully the instrument is installed operational and ready for use 2 4 4 Connecting Optional Devices Optional input devices such as keyboard mouse and barcode reader can be connected to the thermal cycler systems via a USB connection Plug the USB stick into the USB port of the input device Connect the other end of the USB to o One of the USB ports of the GS4M port at rear of instrument is recommended OR o a USB hub if you wish to connect several input devices to a GS4 When connecting several devices plug the cable connector of the USB hub into the rear USB port of the GS4M For further configuration of the device consult the manufacturer s instruction manual 14 Installation G Storm Thermal Cyclers 2 5 AC Power Connection 2 5 1 Operating Power Requirements All G Storm thermal cycler models have self regulating power See Instrument Specifications Power amp Dimensions for power specifications 2 5 2 Fuses WARNING Disconnect the AC power cord before removing or installing a fuse to avoid the possibility of serious in
4. 100V to 240V frequency 48 to 62Hz 1700watts Max 600mm x 600mm x 264mm Weight 32kg approx Operating conditions 10 C 30 0 95 relative humidity Regulatory CE UL CSA compliant Warranty 2 years on all systems G Storm Thermal Cyclers Appendix 53 Compatible Barcodes The following barcodes are compatible with G STORM s documentation system e 2of 5interleaved e Codabar e Code 128 e Code 39 e Code 39 full ASCII e Code 93 full ASCII e EAN 13 EAN 13 with 2 supplement EAN 13 with 5 supplement e EAN 8 EAN 8 with 2 supplement EAN 8 with 5 supplement e EAN 128 e UPA UPA 13 with 2 supplement UPA 13 with 5 supplement e UPE UPE 13 with 2 supplement UPE 13 with 5 supplement e MSI e C I P 54 Appendix G Storm Thermal Cyclers Compatible Plate Formats Gene Technologies recommend using G Storm consumables with G Storm Thermal Cyclers Cat No Product description G Storm RIGID Plates GSR0960 C GS RIGID 96 clear frame clear wells GSR0961 GS RIGID 96 Black Frame white wells GSR0384 C GS RIGID 384 clear frame clear wells GSR0385 GS RIGID 384 black frame white wells G Seals GS 0500 Adhesive Clear Seal GS 0521 Peelable Foil Heat Seal GS 0536 Piercable Foil Heat Seal GS 0541 Clear Heat Seal GS 0550 Adhesive Foil Seal The following plate formats are compatible with G Storm thermal cyclers 55 Appendix G Storm Thermal Cyclers
5. 100 C 100 C Bi EK 90 C soc 80 C 70 C 70 C We Front Right eoc soc 50 C soc 40 C DC 10 C T lt 9 18 09 pm i zeoe jes RESERVED FOR None Figure 5 1 GS4M Cycler Monitor Screen To copy your program onto other cyclers click on the profile pane of the cycler which has your program loaded and click on the WES button Select the additional cyclers on which you wish to run the program by clicking on the appropriate profile panes to highlight them and click on the Paste button Your program is now loaded on to the cyclers Ensure that all appropriate cyclers are selected highlighted and click on the Start button Enter your sample volume 2 150ul and click OK to start the selected program Several cyclers can be controlled simultaneously from the Cycler Monitor screen Click on the profile panes to select highlight the cyclers The buttons below the profiles can be used to control all of the selected cyclers G Storm Thermal Cyclers Run Operation 5 6 3 Viewing Current Program Step Information After a run has started a graphical representation of the temperature parameters is displayed on the Profile Screen In addition detailed program and instrument information can be viewed using the Program or Info buttons located in the right upper corner of the information screen Click Program to display the program steps of the running protocol The executed step is highlighted Click Info to vie
6. Cycler X Power Cable 110 or X 220V Stylus x3 xX G Storm Thermal X 2 2 2 Initial Inspection All parts of the instrument were thoroughly inspected and tested before the unit was shipped therefore the instrument should be in good operating order Carefully inspect the instrument and its accessories for any physical damage sustained in transit If the instrument is received in a damaged condition please file a claim with the transport carrier immediately and contact G Storm for advice G Storm Thermal Cyclers Installation 9 2 2 3 Thermal Blocks The thermal blocks are shipped in separate packaging from the unit They should be removed from their packaging and located into position in the server or satellite unit Ensure that you push down the front and the rear of the block so that the block is fully engaged in its correct position Figure 2 1 GS4M thermal cycler thermal block installation 10 Installation G Storm Thermal Cyclers 2 3 Functional Overview G Storm thermal cyclers are automated instruments designed for carrying out methods for amplification of DNA templates Power switch USB Interfaces for memory stick or keyboard on left side and rear lids Touch screen display Figure 2 2 GS4M thermal cycler profile view The G STORM range is operated via its own internal PC and a universal software application offering independent control of the thermal block G Storm The
7. Storm thermal cycler instruments were developed for operation in laboratories in which there is normal ambient temperature and no explosive atmosphere The ambient temperature should be between 10 C and 30 C the humidity between 0 and 95 2 1 2 Electrical The thermal cycler must be connected to a grounded socket G Storm Thermal Cyclers Installation 7 2 1 3 Thermal Blocks e Reaction vessels should be filled outside the thermal blocks so that no fluids penetrate the instrument e To prevent damage to the blocks and the heated lids make sure you use only recommended sample tubes Unsuitable tubes can be damaged causing the escape of sample material This may present a serious health hazard especially when working with infectious materials e Make sure plates are positioned correctly in the blocks to prevent damage to the blocks and the lids e Ensure that the heated lid is closed when heating tubes in a block otherwise tubes may burst due to high temperatures causing the escape of sample material e The closing lid presents a potential pinching hazard Do not place hands or fingers over the cycler when the lids close otherwise hands or fingers may be caught between the lid and housing 8 Installation G Storm Thermal Cyclers 2 2 Unpacking the Thermal Cycler 2 2 1 Package Contents The following parts are included in delivery G STORM GS4M Cycler User Manual on CD or a USB Stick Thermal
8. Symbols and Conventions 4 Safety Warnings 3 Sales amp Service Centres 60 Saving Programs 33 Script Information command 31 Service and Support button 18 Setting the Lid Pressure 40 Settings button 20 Software Operation 17 G STORM and GSPC Software 17 Required Computer Skills 17 Specifications 53 Compatible Barcodes 56 Compatible Plate Formats 57 High Pressure Heated lid 53 Neworking amp Barcode Options 55 Power amp Dimensions 55 Reports and Validation 54 Thermal Blocks 53 Thermal Engine Characteristics 55 User amp File Management 54 User Interfaces 54 Start Cycle command 31 Starting a Run on a Single Cycler 40 Starting a Run on Multiple Cyclers 41 Starting up the G STORM 14 Status bar 19 Instrument Reservation 19 Time and Temperature display 19 Store command 31 Switching Off the Instrument 45 Symbols and Conventions 4 System Settings 21 Temperature Step command 28 Terminating a Run 43 Thermal Block 11 Time and Temperature display 19 Title bar 18 Login 18 Service and Support 18 Touchdown command 29 60 Appendix G Storm Thermal Cyclers Trouble Shooting If a Power Failure Occurs 49 Trouble Shooting 49 Unpacking the Thermal Cycler 10 Uploading Primer Sequences 47 USB Memory Stick Operation 46 User Accounts G Storm Thermal Cyclers Adding or Modifying Accounts 22 Deleting Accounts 23 User Levels 21 User Login 37 User Management 21
9. plate into the cycler and pressed OK The barcode will be recorded in the GLP and LabBook report and displayed in the nfo section of the Cycler screen Scan Using Barcode Entry Barcode reading using the barcode scanner Scan Using Keyboard Entry Manual entry of the barcode via keyboard Path for worklist Specifies the path for the worklist Lid Temperature This defines the temperature of the heated lid The temperature may range from 112 C to 120 C The minimum increment is 1 C Lid is ON Select this option to activate lid heating The heated lid automatically switches off at temperatures below 25 NOTE Heated Lid commands cannot be used within a cycle G Storm Thermal Cyclers Programming 25 O Automatic Hot Start Automatic Hot Start This step should be incorporated into a program when using a hot start polymerase Select the denaturation temperature and time When running the program the next command will be executed immediately after the denaturation time is elapsed Please also refer to the polymerase manufacturer s recommendations Z Manual Hot Start this step should be incorporated into a program when using a standard polymerase Select the denaturation temperature and time When running the program the instrument will notify you after the denaturation time has elapsed You will then be asked to open the lid and add the enzyme The instrument timer is now on hold and will continue only after
10. you have pressed Manual Hot Start HE o Temperature Step Use this command to select the required temperature in C and to define the step duration Processing Temperature Set the required temperature NOTE Temperature steps which are within a cycle must be 25 C or above 26 Run Operation G Storm Thermal Cyclers Hold for Set the required time in hours minutes and seconds If the hold at this temperature is option is selected the cycler will remain at the temperature until the program is resumed This allows the user to determine when to continue to the next step Temperature Ramp Rate of change of the sample temperature Rates can be from 0 1 C to 3 C per second Unit is defaulted to run at maximum ramp rate Temperature Increment Temperature increment and decrement per cycle Steps can be from 0 1 C to 10 C NOTE For decrement use negative figures e g 2 Time Increment Time increment per cycle in seconds Step Type Indicates the selected step type for information purposes The supplied step type information will then be displayed in the cycler monitor Touchdown Touchdown is an advanced technique used to reduce non specific binding With the G Storm thermal cycler Touchdown is provided as a one step command for easy implementation in the programming sequence Denaturation Temperature Defines denaturation temperature Maximum Annealing Temperature Sets the maximum annealing temperat
11. 8 2 2 Unpacking the Thermal El enge iesst ee dg Sdedeeed Ae gege SE 9 2 2 1 Package EISES eet edit leek cave eeEeeh EE gaseeensevaeeetstnaniteys 9 2 2 2 Initial Inspection NEE 9 2 2 3 Thermal Blocks snnssessnonoenrnrnnnnnrrrnrrunnnnnunnnnnnnnnseseteenneesne 10 2 3 Functional EIERE de Ee 11 2 A Ee 13 2 4 1 Starting up the G GTODM en 13 2 4 2 Logging in as Administrator sc ceccteseeeeniieniine eines 13 2 4 3 Cycler Validation Tests voxcucnseia ceased tenes ata 14 2 4 4 Connecting Optional Devices AAA 14 2 5 AC Power Connection TEE 15 2 5 1 Operating Power Heouirements AAA 15 G Storm Thermal Cyclers 3 1 3 2 3 3 3 4 4 1 4 2 4 3 4 4 4 5 4 6 2 5 2 TEE 15 2 5 3 Grounding Requirements 22 4 d er euer Seed Aeed EE AE 15 Software Operation Eege 16 G STORM and GSPC Software gene Sege Zeie ENEE EES 16 Required Computer UE 16 Main Screen EE 17 3 3 1 RRE 17 3 3 2 Status teil 18 3 3 3 Command BUTONS hesctestc ats states Se stencteay a T 18 3 3 4 e Le wf Tee E 19 ER EE 20 3 4 1 Cycler Management cs cccseccchs cieensissentenesacteneadescetdenntacttaensdaael 20 3 4 2 User MEHN acess 20 d User Levels sirenerne KE EAK Ee 20 Z422 New USES need Eechen eg gege EA 3 4 2 3 Adding or Modifying User Accounts 21 3 4 2 4 Deleting User Accounts scsi casicce ss celeecas se sdcctsedereceapeycescedet edeee 22 3 4 3 Nier ZEIEN dh 22 Programming EE 23 Creating Proglas EE 23 4 1 1 DND Programming ae deeg eege een ddee
12. B Memory Stick Operation c ccseeceessseeeeeesseneeeeeeeeeeeeeeneeeeeeeeneeeees 43 Importing and Exporting dl 43 6 1 1 Exporting Program Files to a memory Stick ceeeees 43 6 1 2 Importing Program Files from a memory stick to the GS4 43 6 1 3 Uploading Primer Sequences from a memory eck 44 Archiving Reports EE 44 Maintenance sssssnsssssennnunnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnn nnnnnnnnn nnmnnn 46 STE le EE 46 TrOUBIE SHOOUNG sien eer ee ee eee 47 If a Power Failure OcGurs viii cisc cissccteresnenseesnchendaesotkeenessardeatevannaleatenendenate 47 Troubleshooting Information EE 48 Block Exchanges otitis isc isteutaneaca ci ecatieieatsaaanuacaumoativaiconstecinecaiteriansnsantocatves 50 Removing the EEN 50 Gene Technologies Thermal Cycler Inserting the lge ege ee 50 ADDON TEE 51 Instrument Specifications ugereegt sxeasncescunnsucet caplideeddcasnnneeat caesaneenmeans 51 Thermal Bloeke2essggbtzessgtesn izege dees Sege Seas ee Ee 51 Heated Lig WE 51 User Interface S iranin iar eea A A AR ed dat 52 User amp File Management c ceecceceeeeeeeeeeeeeeeeee errr ee eeseeeeeeeeeeeeeeneees 52 Reports and Validation isccccescdeiendnsee iii thei ae 52 BarCOdesO Pron E 53 Thermal Engine Charachertetce AAA 53 Eerst eege dees 53 Compatible Barcodes iic csccdeie deaceesekanc tev eitaesissictidenweisrediedeeadestiees 54 Compatible Plate Karakter egene tee 55 Order Information Geet
13. G STORM 5S G STORM GS4M Instruction Manual G Storm 2011 Release Information This document is protected by copyright All Rights Reserved G Storm Somerton Biotechnology Centre Ricksey Lane Catcombe Somerton Somerset TA11 7JH England Phone 44 0 7919 121510 Email info gstormdirect com Web www gstormdirect com Manual Version 1 0 Trademark Information All product and brand names used in this document are trademarks or registered trademarks of their respective holders Gradient options are not available in The USA Japan and Germany Copyright 2011 G Storm G Storm Thermal Cyclers Contents General IRTORMIAtION EE 1 ele EE 1 VE elen EEN 1 1 Safety Warnings and Precautions ccccssseeeeeeeseeeeeeeeseneeeeeeeeneeneeeeeees 2 1 1 Before Operating the INStruMment se cccccsdeceetees ded ethcencede hiss EE AED 2 1 1 1 Instr ment Safe ea eea Sean aar apero a aa EAER EENEN ERRA TREA 3 1 1 2 Symbols and Conventions cccceccceeeeeeceeeeeeeeeeeeeeeeeeeeeeeeeees 3 1 1 3 Potential Safety Hazards scscccccasccrscennds sitions decuteadetiaerstiatiadt 4 Aes fee Py EC OTR 2 ue eege 4 1 1 3 2 EXPLOSIVE elle ENEE deed dd 4 1 1 3 3 PINGHING HAZARIK erugeet desst ANERER Eug 5 ga Be Et ET 5 Jet en DANGER OF BURNS egene n eu cheater tee 5 1 1 3 6 OPERATINGENVIRONMENT 5 2 Installation EE 7 21 FREQUIPSINGINS EE 7 2 1 1 HEEN 7 2 1 2 tte EEN 7 2 1 3 Thermal BlOCKS niiina aeaii
14. Input commands cannot be programmed inside a cycle e Only a single Script Information command is valid within a program The following commands or command combinations are considered stages e Automatic Hot Start e Manual Hot Start e A string of up to ten single Temperature steps e Single Gradient steps e A complete cycle including Temperature or Gradient steps within a start cycle end cycle pair e A Touchdown command e A Store command When using these stages in a script the following rules apply e The maximum number of stages within a script must not exceed 10 e The maximum number of Temperature and Gradient commands inside a cycle must not exceed 10 e The maximum number of cycles within a stage is 99 G Storm Thermal Cyclers Programming 31 4 3 Saving Programs To save a program script click on Save As in the Compose Edit a Program window Enter a program name and click Save to save the file to your user directory Users can create folders within their user directory to organise their programs After entering the program name click Create Folder enter a folder name and click OK Select beside the Scripts folder to show all available folders and click on the newly created folder Click Save to save the program script to this folder A program can also be saved to an existing folder by selecting it as described above 4 4 Editing Programs 4 4 1 Editing Program Parameters You can edit the
15. ND Programming and the Program Wizard 4 1 1 DND Programming Drag and Drop programming DND programming is an easy and intuitive method for conveniently creating programs step by step The basic idea of DND programming is that you simply choose the required command from a command list drag it with the mouse into the program window on the left and drop it into the desired program position This opens a window where you can define further command specific settings such as time temperature lid pressure etc In this way you can create your program command by command according to the planned programming sequence The command order can be changed easily any time just by dragging the selected command to the desired position within the program window Once the program is started the cycler will process the commands from top to bottom according to the defined programming sequence To generate a new program using DND click Q on the Home Page This will open the Compose Edit a Program screen with its drag and drop functions G Storm Thermal Cyclers Programming 23 E PCQB Untitled G sTo RM ComposelEdit a Program Program Window H List Home Page 27 Close Lid The Current Program is valid Run Time 00 00 00 e Profile 2 UI OI Barcode Input Cancel D Script Information SE Actual Target dd 4 32 34 pm E np E Be RESERVED FOR None Figure 4 1 Compose Edit a program screen The Compose Edit a Progr
16. VED FOR field in the right corner of the bottom status bar e Select Reserve next run for Current User if you are already logged in o If you are not logged in select Reserve next run for User to reserve the subsequent run o Select your user name from the Username drop down list and enter your password e Click on Reserve to confirm the reservation 5 3 1 Cancelling a Reservation To cancel an existing reservation check the Clear Reservation button To quit the reservation window without saving any changes click Cancel 5 4 Viewing Script Temperature Profiles Prior to Run Before starting a run you can view the temperature profile of a new or stored program Click on Profile in the Compose Edit a program screen window An information screen is displayed detailing all the parameters of the selected program 36 Run Operation G Storm Thermal Cyclers 5 5 Loading the Cycler Press the Open Close button on the front of the instrument or click Q on the start screen to access the block WARNING A Danger of Burns The thermal block test tubes and plates may reach temperatures as high as 100 C Before loading or unloading the cycler keep hands away until temperature reaches 30 C or less Pinching Hazard Do not place hands or fingers in to the cycler when the lid is being closed 5 5 1 Loading Plates Depending upon the thermal block installed the thermal cycler can be loaded with one 96 well plate or one 384 wel
17. View Edit Program button 19 Viewing Program Profile 42 Appendix 61
18. a tere cre tere a Tenet nen err eee een 57 Sales amp Service ue Error Bookmark not defined ue EE 59 G Storm Thermal Cyclers General Information Policy Statement It is the policy of G Storm to improve products as new techniques and components become available G Storm reserves the right to change specifications at any time Warranty Statement G Storm guarantees that the thermal cycler you have received has been thoroughly tested and meets its published specification This guarantee is valid for 24 months only if the product and functions have been used according to the instruction manual No liability is accepted for loss or damage arising from the incorrect use of the thermal cycler G Storm s liability is limited to the repair or replacement of the unit or refund of the purchase price at G Storm s option G Storm is not liable for any consequential damages G Storm s thermal cyclers are for research use only Read the Instruction Manual carefully before using the thermal cycler to ensure that you obtain the best possible results from the machine G Storm thermal cyclers should only be used by suitably qualified and trained people If the thermal cycler is not used as specified in this manual the protection provided by the equipment may be impaired G Storm Thermal Cyclers General Information r 1 Safety Warnings and Precautions 1 1 Before Operating the Instrument Ensure that anyone involved with the op
19. am screen consists of the Command List which contains all available program steps and the Program Window where you can create or edit programs 1 Select a command from the Command List then drag and drop it into the Program Window This will open a new window for the selected command 2 Enter your settings in the relevant fields of the selected command 3 Confirm your settings by clicking OK This will return you to the Compose Edit a program window To add another command to your program repeat steps 1 3 for each required command Once you have finished creating your program save it by clicking on Save As and you will be prompted for a file name Name your file within Windows naming conventions Click on Profile if you wish to view a graphical representation of the defined temperature profile 24 Run Operation G Storm Thermal Cyclers 4 1 1 1 Program Steps The following program steps are available in the Command List for creating customised programs in the Program Window ir 123 Use this command to define barcode settings When composing the program Barcode Input it is recommended to place the barcode command at the beginning During program run you will be asked to read the plate s barcode You may do this with a handheld USB barcode scanner or by manually entering the barcode If you use the barcode scanner the barcode cannot be edited after reading The program will continue after you have placed the
20. ck A Removing the Block Remove the block by simply holding it with two hands on the left and right side and lift it up Figure 9 1 Exchanging the block Inserting the Block Hold the block with two hands on the left and right side with the block connector at the back of the block and place it into position in the thermal cycler G Storm Thermal Cyclers Block Exchange 50 Appendix Instrument Specifications Thermal Blocks Block Materials Modular anodised aluminium blocks with 4 sensors Traceability NIST traceable temperature calibration Blocks Available 96 well gradient block For 96 well plates or 96x 0 2 ml tubes 96 well Fast gradient block For 96 well plates 96 x 0 2 ml tubes 9384 well gradient block For 384 well plates 9384 well Fast gradient block For 384 well plates In situ block for 4 slides Heated Lid Lid Temperature Range 112 C 120 C user selectable in 1 C increments Lid Pressure Range Manual lid pressure setting Compatible with all vessels amp seals Ambient temperature 10 C to 30 C Relative Humidity 0 to 95 G Storm Thermal Cyclers Appendix 51 User Interfaces Screen Type 8 4 inch TFT colour touch screen stylus supplied Touchscreen external keyboard Optional amp mouse USB Optional barcode scanner USB Optional System Input Temperature Display Real time graphical display of actual amp set block tempe
21. dE eget eg 23 ST Program Steps sonst eater eel a a a A 25 4 1 2 Program Wizard EE 30 General Programming Guidelines AAA 31 Saving Pr gramMS EE 32 Editing dein 32 4 4 1 Editing Program Parameters c ceeeccceeeeeeeeeeeeereeeeeeees 32 4 4 2 Deleting a Program Step EE 33 4 4 3 Converting a Gradient Gren 33 Deleting ProgramS EE 34 Incubate ul EN 34 PUR OPGFatlon eege Ee 35 G Storm Thermal Cyclers 5 1 5 2 5 3 5 4 5 5 5 6 5 7 5 8 5 9 6 1 6 2 7 1 8 1 8 2 User Be Un BEE 35 5 1 1 EE EE 35 5 1 2 Registered Users a catesseveesicce sates Saavenyentacnteadeneyanngseeredeeuedetadnotene 35 Changing USers as tee co cant See cattent eltagcta ag Medea caaes tinal aon thes See esteadaes 35 Making an Instrument Reservation c cecccceeeeeeeeeeeeeeeeeeeeeeeeneeeeseees 36 5 3 1 Cancelling a Reservation VE 36 Viewing Script Temperature Profiles Prior to Hun 36 Loading the TEE 37 5 5 1 Loading Plates issii aa E ias 37 5 5 2 Loading Re 37 Program E BEE 38 5 6 1 Starting a Run on a Single Cycle 38 5 6 2 Starting a Run on Multiple Cvclers 38 5 6 3 Viewing Current Program Step Information ceeeeeeee 40 5 6 4 Terminating a Run cries eee techn Cases ties fae ad eed arene cee oes aude aed 40 SEET 41 5 7 1 Viewing H pertg enee leede E eege 41 Language Selection EE 42 Switching Off the Instrument ss ceccceessadeceedescseacenss nc crnenestnawersendirecesnedenetnenaye 42 US
22. e Archiving maintenance of all reports 3 4 2 2 New Users Only the administrator is authorised to set up new users and to change or assign user rights and passwords As a first time user you will be logged in as Guest user visible in the top status bar Guest users usually have only restricted user rights and work from the unprotected Guest folder which can be accessed by all users Before you can operate the thermal cycler as a regular user your administrator has to set you up as a new user Once you are a registered user you have a personal encrypted file folder where you can administer your own program files 3 4 2 3 Adding or Modifying User Accounts NOTE Only the administrator can set up new users or modify existing user accounts To add a new user click New User to open the Add a new User screen Assign a user name and password Select the required user level Guest User Administrator from the drop down list Now you can define the user s script reading and writing privileges by checking the appropriate boxes Edit Rename Script The user has the right to edit or rename a program file Delete Script The user has the right to delete program files Create Script The user has the right to create program files G Storm Thermal Cyclers Software Operation 21 Once you have entered all data click on Create User to save the settings After successful setup a confirmation message will be displayed and the n
23. electrical safety precautions should be applied e Ensure that the proper voltage is supplied before turning the instrument on for the first time e The device must be connected to a grounded socket e Do not touch any switches or outlets with wet hands e Switch the instrument off before disconnecting the AC power cord e Unplug the instrument prior to cleaning up any major liquid spills and prior to servicing any of the electrical or internal components Only qualified personnel should perform electrical servicing 1 1 3 2 EXPLOSIVE SUBSTANCES Explosive flammable and reactive substances should never be cycled or incubated in any thermal cycler Do not operate the thermal cycler in a hazardous or potentially explosive environment You must observe the relevant safety regulations when handling pathogenic material radioactive substances or other substances hazardous to health 4 Safety Warnings and Precautions G Storm Thermal Cyclers 1 1 3 3 PINCHING HAZARD The closing lids present a potential pinching hazard Do not place hands or fingers over the cycler when the lids are being closed 1 1 3 4 FLUIDS Reaction vessels should be filled outside the cycler so that no fluids penetrate the instrument Do not submerge the instrument in any liquid 1 1 3 5 DANGER OF BURNS The thermal blocks inner side of heated lids and reaction vessels AN quickly attain temperatures of greater than 50 C Do not touch Ris
24. ents 8 Thermal Block 11 Instrument Reservation 19 38 Language Selection 45 Lid 25 Ligation reactions 36 Lightning flash 20 Loading the Cycler 39 Logging in as Administrator 14 Login button 18 Main Screen 13 18 Maintenance 48 Manual Hot Start command 28 New Program button 19 New Users 22 Operating Power Requirements 16 Operation 37 Cancelling a Reservation 38 Changing Users 37 Instrument Reservation 38 Running a Program 40 Sample Loading 39 Terminating a Run 43 User Login 37 Viewing Program Profile 42 Appendix 59 Viewing Script Temperature Profiles Prior to Run 38 Optional Devices 15 Order Information 59 Package Contents 10 Password 14 Program Execution 40 Program Steps 26 Automatic Hot Start 27 Barcode Input 26 End Cycle 31 Gradient Step 30 Lid Pressure 26 Manual Hoistart 28 Script Information 31 Start Cycle 31 Store 31 Temperature Step 28 Touchdown 29 Program Wizard 32 Program Wizard button 19 Programming 24 Converting a Gradient Step 35 Deleting a Program Step 35 Deleting Programs 35 Editing Programs 34 General Guidelines 32 Program Wizard 32 Saving Programs 33 Reports 44 Archiving Reports 47 Viewing Reports 44 Reports button 20 Requirements 8 Run Program button 19 Running a Program Starting a Run on a Single Cycler 40 Starting a Run on Multiple Cyclers 41 Safety Hazards 5 Instrument 4
25. eration of the instrument is instructed in both general safety practices for laboratories and specific safety practices for the instrument Always place the instrument in a location where if necessary the main power supply can be disconnected immediately 2 Safety Warnings and Precautions G Storm Thermal Cyclers 1 1 1 Instrument Safety Please read this section before operating the thermal cycler Operators of this instrument must be trained in both general laboratory safety practices and the specific safety requirements of the thermal cycler If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired All functions performed within the context of preparing performing and completing a run should be done with caution and care and with general respect both to the instrumentation and to associated chemicals samples and other devices 1 1 2 Symbols and Conventions The following chart is an illustrated glossary of the symbols that are used in this manual bd CAUTION This symbol indicates a potential risk and alerts you to proceed with caution A CAUTION This symbol indicates the presence of high voltage and warns the user to proceed with caution A CAUTION This symbol indicates risks associated with hot surfaces G Storm Thermal Cyclers Safety Warnings and Precautions 3 1 1 3 Potential Safety Hazards 1 1 3 1 ELECTRICAL Standard
26. ew user name will be added in the User Management list To modify an existing user highlight the user name and click Edit User to open the Edit a User screen Here you can modify user data such as name user level password user privileges and script directory protection Click OK to save the changes A confirmation message will be displayed after successful update of the user data 3 4 2 4 Deleting User Accounts NOTE Only the administrator can delete User accounts The User Management screen lists the available user names and corresponding user levels Select the user name you wish to remove Click on Remove User to remove the user name from the user list It removes all user permissions that the user has and does not allow that user to access any the thermal cycler device It also deletes all scripts and GLP reports associated with that user NOTE The Remove User command also deletes all scripts and GLP reports associated with that user The Administrator user account cannot be deleted 3 4 3 Cycler Settings Browsing this option allows an Administrator to edit date and time settings and select the language for the instrument The Power Resume On Off option determines whether a program should continue or not after a power failure 22 Run Operation G Storm Thermal Cyclers 4 Programming 4 1 Creating Programs G STORM and GSPC software offers two innovative ways of composing programs Drop and Drag Programming D
27. ing up the instrument the default user level is Guest If you wish to continue to operate the cycler with Guest user rights no special login procedure is required In this case you can access the unrestricted Guest folder and operate the cycler as described in the relevant chapters 5 1 2 Registered Users Once the administrator has set you up as a registered user see 3 4 2 3 you can operate the thermal cycler using your personal data and file folders To log into the system as a registered user proceed as follows e Click on Login the Login screen opens e Select your user name from the Username drop down list and enter your password e Click on the Login button This will log you into the system giving you reading and writing rights as specified for your user level e After successful login your user name is displayed in the top status bar NOTE In case a user forgets his or her password the logged on administrator can assign him her a new one 5 2 Changing Users In order to log into the system when another user is already logged in proceed as described in section Registered Users above G Storm Thermal Cyclers Run Operation 5 3 Making an Instrument Reservation The thermal cycler is equipped with a visual reservation function The reservation is not blocking or restricting the instrument to any user It only indicates that the instrument is needed for a certain user after the current run e Click on the RESER
28. jury from electrical shock Fuse compartments are located on the rear of the instrument above the main power connection Check the voltage rating label to verify that the instrument is compatible with the AC voltage available at the installation site 2 5 3 Grounding Requirements DANGER please read carefully To protect operating personnel the National Electrical Manufacturers Association NEMA recommends that the instrument be correctly grounded The instrument is equipped with a 3 conductor AC power cord that when connected to an appropriate AC power outlet grounds the instrument To preserve this protection feature do not operate the instrument from an AC power outlet that has no ground connection G Storm Thermal Cyclers Installation 15 3 Software Operation 3 1 G STORM and GSPC Software G STORM thermal cyclers are operated via their own internal PC running under Windows and a universal software engine offering simple and intuitive instrument control The unique Program Wizard function enables stepwise protocol definition with no tedious calculations required Drag and drop icon based commands make all steps quick clear and intuitive throughout Optimised conditions from gradient protocols are converted to standard protocols with only a single point and click The programming software can be installed additionally on any laptop or desktop PC running WIN 2000 or XP The memory stick supplied allows quick and easy da
29. k of Burns Keep the heated lid closed until temperatures of 30 C or lower are reached Do not use any materials plates sealings foils mats which are not sufficiently temperature stable up to 120 C 1 1 3 6 OPERATING ENVIRONMENT Please ensure that the ventilation slots of the device remain free to vent at all times A space of at least 10cm should be left around the thermal cycler The ambient temperature should be between 10 C and 30 C the humidity between 0 and 95 G Storm Thermal Cyclers Safety Warnings and Precautions 5 WARNING G Storm trained service engineers must carry out all repair work Only original G Storm replacement parts must be used Disconnect the instrument from the mains supply before opening 6 Safety Warnings and Precautions G Storm Thermal Cyclers 2 Installation 2 1 Requirements The following steps must be followed for optimal and safe operation of the thermal cycler 2 1 1 Site The thermal cycler should be placed on a rigid flat clean surface Make sure that the instrument is completely stable Adequate ventilation is important Make sure there is sufficient space so that the rear and side air slots are not covered and to allow cooling air to circulate freely around the instrument There should be no paper under the device as this may block the ventilation path The unit should always have at least 10cm distance to the next wall or neighbouring instrument G
30. l plate Ensure the plates are used in conjunction with a suitable seal Put your plate on the block and ensure that it is correctly positioned in the provided space 5 5 2 Loading Tubes G STORM thermal cyclers can be loaded with up to 96 tubes 0 2 ml when using the 96 well block G Storm Thermal Cyclers Run Operation 37 5 6 Program Execution 5 6 1 Starting a Run on a Single Cycler To start a run e Select a cycler by clicking on the appropriate Cycler button and click on the Home Page Your personal file folder opens or if you are logged in as a guest the guest folder opens displaying all available programs Select the program you wish to run Click Run selected on the right hand side of the window enter your sample volume 2 150ul and click OK to start the selected program 5 6 2 Starting a Run on Multiple Cyclers Select one of the cyclers you wish to use by clicking on the appropriate Cycler Program button Click on the Home Page and open the program which you wish to run Your program is now loaded on to this cycler Click on the Cycler Monitor button to bring up the Cycler Monitor screen Figure 5 1 The profile panes of all four cycles are displayed 38 Run Operation G Storm Thermal Cyclers merce and Support Cycler Ba G STORM End Time 10 55pm Program TEST End Time 10 55pm Program TEST Home Page Auge Er Time 10 65 pm Program TEST was Program TEST f
31. ling Temperature To generate a new program click on Program Wizard on the Home Page 1 Primer Information Enter either primer sequences or melting temperatures Sequences can be uploaded from a txt file on a memory stick Click Browse in the Sequence Primer One Sequence Primer Two section and browse for the required files in the Upload Sequence from USB Memory Stick screen window Select the desired file and click Open to upload the file to the thermal cycler 2 Product Length Enter the length of your product either manually or using the slide bar 3 DNA Source Information Select from Bacterial Eukaryotic or Plasmid 4 Specials Select any special requirements that your program may require e g type of enzyme high AT GC content 5 After you have supplied all required data click on Generate Program The Compose Edit a program screen window opens where you can view and edit the program steps To edit a program step double click the respective command To view a graphical representation of the defined temperature profile click Profile 30 Run Operation G Storm Thermal Cyclers 4 2 General Programming Guidelines To ensure a correct run the following guidelines should be followed when composing a new program e Aprogram must contain at least one Temperature Step e Cycles cannot be nested A started cycle must have an End Cycle command before starting the next Start Cycle command e Heated Lid and Barcode
32. mage and select the best performing column e Double click the Gradient Step command e Select the Convert Gradient Step to a standard temperature step checkbox e Select the appropriate column from the drop down list in the Conversion section The thermal cycler software will now convert the gradient step into a temperature step and automatically select the right temperature in the chosen column G Storm Thermal Cyclers Programming 33 NOTE If it is required to repeat the gradient experiment the converted temperature step can be set back to a gradient command just by unchecking the Conversion option 4 5 Deleting Programs To delete an existing program rogram e Click on the Home Page Depending on your user level guest or registered user the specific user directory or guest directory is displayed listing all existing program files Select the program you wish to remove Click Delete This will remove the program from the script folder 4 6 Incubate temperature To run the cycler for incubations or ligations utilise the Incubate temperature function 7 Incubate e Click Gi on the Home Page e Select the temperature that you require e Select the time you require OR e Select infinity Cycler will run until you Stop the program e Select the heated lid if required and the pressure setting 34 Run Operation G Storm Thermal Cyclers 5 Run Operation 5 1 User Login 5 1 1 Guest Users When power
33. o cleaning The electrical safety fuses are located above the main power switch and the main power plug at the rear of the device Before the fuses are replaced the device must be switched off and disconnected from the mains supply Only fuses with the correct voltage values may be used Only qualified service personnel should perform servicing of the thermal cycler No warranty obligation is assumed by G Storm in the event of damage caused by unauthorised servicing 46 Run Operation G Storm Thermal Cyclers 8 Trouble Shooting 8 1 Ifa Power Failure Occurs All G Storm thermal cyclers are equipped with an auto power fail restart function that allows for power failures and safe continuation of a run after resumption of power if set to do so in the cycler settings The autopower fail restart function restarts the script from the beginning of the temperature step in which it failed The following table lists the three power failure scenarios and the actions the instrument takes if the power is interrupted during operation Observation Explanation Remedy Actions The computer fails but the In this case the thermal engine will continue to thermal cycler unit still has execute the script without any interruption power The thermal cycler fails but The execution of the script will be affected and will the computer still has power halt whilst the power is off Once the power resumes restart the unit and the script will re
34. on in tubes during arun Condensation will appear naturally at the end of a run and has no deleterious effects Simply spin or flick the liquid back to the bottom of the tube and continue with the post reaction steps However condensation during a run will result in sub optimal cycling If this is experienced try increasing the lid temperature Sealed microplate losing sample volume Poor quality microplate sealing system or inadequate pressure USB devices including memory stock and barcode scanner not recognized Reboot machine Machine does not power up and power light is not illuminated Check power is on at supply Check fuses at rear of instrument above power inlet Blank Display lit but showing no information Reboot machine If the problem persists there is an internal connection problem call Technical Support Abnormal block heating cooling performance Run Thermal Engine OQ test to check performance 48 Run Operation G Storm Thermal Cyclers 1 Please refer to the thermal cycler IQ OQ Protocol document for instructions on performing instrument qualification tests 2 The Temperature Acquisition System TAS can be purchased separately G Storm Thermal Cyclers Trouble Shooting 49 9 Block Exchange Block exchange can be performed within seconds No tools are required for this purpose CAUTION A Turn power off and interrupt power line before changing the blo
35. on successful reservation the name of the subsequent user is displayed in the status bar 3 3 3 Command Buttons The Home Page Window comprises command buttons which provide quick access to the functions that are available on the GS4M Run Program opens file list enabling users to select program to run New Program opens the Compose Edit a Program screen eg View Edit Program opens the file list to browse programs Incubate opens the Incubate Temperature screen Program Wizard opens the Program Wizard screen where you can generate a program by entering biological information 18 Run Operation G Storm Thermal Cyclers Reports opens the report browser where you can search for stored reports Settings opens the System Settings screen where you can view amp edit cycler settings manage users and run cycler tests 3 3 4 Additional functions G STORM This logo serves as a Home button Clicking on it Home Page from any screen returns user to the Home Page Displays status information of all cyclers and allows user to control multiple cyclers simultaneously The cycler buttons should also be used to select a A cycler prior to running a program Double clicking Back Right these buttons displays the Status screen for each Front Left cycler If a program is running the Run Profile is displayed Front Right Provides the operational status of each cycler wi flashes green when the instrument is running G St
36. orm Thermal Cyclers Software Operation 19 3 4 System Settings Specific system settings can be defined by clicking on the Settings button on the Home Page screen when logged in as an Administrator Use the Settings menu to add new thermal cycler devices or edit existing units set time and date run cycler tests or manage user accounts 3 4 1 Cycler Management Browsing this option allows an Administrator to edit cycler settings and perform cycler validation tests 3 4 2 User Management Browsing this option allows an Administrator to manage cycler users 3 4 2 1 User Levels Three different user levels are available e Administrator e Registered User e Guest User Each level has specific user rights Only administrators are authorized to set up new users and to change and or assign user rights and passwords By default the thermal cycler starts with Guest user rights User Rights Functions allowed Guest User e Access rights to the Guest folder e Creating copying editing and executing programs stored in the Guest folder Registered User e Access rights to the Guest and personal user folders 20 Run Operation G Storm Thermal Cyclers e Creating copying editing and executing programs stored in the Guest and personal folders e Access to GLP reports denied Administrator e Access rights to all Guest and user folders e Setup and maintenance of users e Setup and maintenance of cyclers
37. oup the displayed reports by selecting one of the sorting criteria below Group by Date Sorts records in ascending order by date Group by Cycler Sorts records by cycler name Group by Program Sorts records by program name For viewing GLP and or LabBook reports select one of the following options Show GLP Displays the generated GLP records only for users with administrator rights Show LabBooks Displays the generated LabBooks 41 Run Operation G Storm Thermal Cyclers 5 8 Language Selection G Storm thermal cyclers can be operated in English Default and French To change the default language e Login as an administrator e Click Settings on the Home Page e Click Browse on Cycler Settings e Select the language you require e Click OK The unit will then remain in the selected language 5 9 Switching Off the Instrument The unit can be switched off at any time during operation using the power button at the back of the instrument There is no need to exit the thermal cycler software prior to switching off the instrument 42 Run Operation G Storm Thermal Cyclers 6 USB Memory Stick Operation 6 1 Importing and Exporting Files You can import Primer sequences and programs created on a different thermal cycler or on a PC to the G STORM thermal cycler and GSPC software or export programs and reports that have been created using a USB memory stick 6 1 1 Exporting Program Files to a memory stick e Make sure
38. parameters of existing programs and store the program with the same name or rename the edited program To edit an existing program L Program e Click on the Home Page Depending on your user level guest or registered user the specific user directory or guest directory is displayed listing all existing program files Select the program you wish to edit e Click Open Opening the file automatically loads the program and the program steps are displayed in the Program Window of the Compose Edit a Program screen e Double click the command you wish to edit This will open the window of the respective command e Edit the parameters as needed 32 Run Operation G Storm Thermal Cyclers e Click OK to save your changes and to return to the Compose Edit a Program screen e After you have modified all data click Save in the Compose Edit a Program screen if you wish to store your changes in the existing program e Click Save As if you wish to rename the program You will then be prompted for a file name 4 4 2 Deleting a Program Step To remove a program step from the program just drag it from the Program l Window and drop it into the recycle bin icon located below the Program Window Save the program as described above 4 4 3 Converting a Gradient Step A gradient step can be converted to a standard temperature step with a few mouse clicks e After performing the gradient experiment have a look at the gel i
39. ratures 1x USB front personal memory stick Communication Interfaces keyboard barcode reader mouse USB hub 1x USB rear User amp File Management User Level Management 3 levels Administrator User with administrator selectable restricted rights Guest User File Protection Yes for programs and reports File Organization Windows Explorer user defined folders amp subfolders Program Storage Minimum 99 internal memory or USB stick Circa 500 Unlimited PC program storage Reports and Validation Report Function Encrypted GLP report LabBook report Validation Internal auto validation prior to each program start Thermal engine validation function 52 Appendix G Storm Thermal Cyclers Barcode Option Networking Barcode Option PC controlled cycler network up to 15 units via USB hubs Barcode documentation via handheld barcode scanner Thermal Engine Characteristics Temperature Control Sample Volume Range Sample Accuracy Sample Homogeneity Heating Cooling Sample Overshoot Gradient Temperature Range Maximum minimum Gradient Span 4 99 C with simulated volume dependent control algorithm 5 150ul 0 4 C 20 99 C 1 C 4 20 C 0 4 C Up to 3 C per second Up to 3 C per second lt 1 30 C 80 C 30 C 1 C Power amp Dimensions Electrical Power Supply Dimensions LxWxH
40. rmal Cyclers Installation 11 Service and Support G sTo RM Home Page 4 View Edit Program lt 9 18 09 pm jes RESERVED FOR None Figure 2 3 GS4M thermal cycler Main Screen Home Page 12 Installation G Storm Thermal Cyclers 2 4 Cycler Installation 2 4 1 Starting up the G STORM Once you have set up the G STORM as explained in section 2 1 and 2 2 you can connect it to the mains e Plug the AC power cord into the main power connector at the rear of the instrument and connect the other end of the cable into a grounded AC outlet e Press the power switch on the side of the instrument e The cooling fan powers up and the thermal cycler will go through a short initialisation routine Then the Home Page appears providing access to the thermal cycler s navigation elements 2 4 2 Logging in as Administrator Only the Administrator is authorised to change cycler settings and run the cycler validation tests 1 Click on the Login button on the Home Page 2 Select Administrator from the Username drop down list and enter the administrator password The default administrator password is admin 3 Click Login 4 You are now logged in as Administrator visible in the top status bar IMPORTANT It is recommended that the factory set login password be changed to prevent unauthorised access to the instrument see section 3 4 2 3 G Storm Thermal Cyclers Installation 13 2 4 3 Cycler Validation Tests
41. sume from the beginning of the step it was executing when the power failed Both the computer and the The execution of the script will be affected and will thermal cycler unit lose halt whilst the power is off Once the power resumes power the script will resume from the beginning of the step it was executing when the power failed Corrective action Restart the application and the connection will be restored 47 Trouble Shooting Gene Technologies Thermal Cyclers 8 2 Troubleshooting Information Refer to the following table for a description of potential problems and recommended actions that you should take Observation No actual temperature displayed at the bottom of the application Explanation Remedy Actions Block not correctly inserted in the machine Check that the block is inserted correctly and reboot the machine Poor experimental yield 1 Run Thermal Engine OQ test to check that thermal engine operation is within specification 2 If transferring protocol from a different make of thermal cycler use TAS system to determine new protocol parameters Program does not start Block not correctly inserted in the machine Check that the block is inserted correctly and reboot the machine Experiments work with tubes but not with plates or vice versa Make sure correct lid pressure is applied Tubes are crushed Applied pressure too high Condensati
42. ta and program exchange between PC and the thermal cycler s internal PC 3 2 Required Computer Skills Users should have basic computing knowledge including using the mouse selecting items and drag and drop 16 Software Operation G Storm Thermal Cyclers 3 3 Main Screen Upon power up of the system the screen first displays a start up routine followed by the Main Screen Home Page The Home Page is the main location for entering and displaying information Service and Support G STO RM Home Page View Edit Program a a Front Left Command Buttons Cycler Monitor Actual lt 9 18 09 pm E 86 0 C E Bez RESERVED FOR None Status Bar Figure 3 1 GS4M Main Screen with navigation elements 3 3 1 Title Bar The Title Bar displays general information Displays contact details for service and application support Cycler Displays the currently selected cycler Program Displays the currently loaded program of the selected cycler User Displays the current user Displays the User Login screen G Storm Thermal Cyclers Software Operation 17 3 3 2 Status Bar The Status bar displays the following information Displays information on the current time SEH De Target and temperature and defined target D 125047 am E one 3 e Se temperature of a program step Opens the Reservation screen where you Can reserve the thermal cycler for RESERVED FOR None the next run Up
43. the USB memory stick is inserted in the USB slot on the side of the instrument e Click View amp Edit Files to display your personal file folder Files can only be transferred individually e Highlight the desired program by clicking on it once e Click Archive Programs e Click View USB to check that the file has been transferred NOTE the lock button on the memory stick may prevent data access Please ensure the lock button is in the unlocked position 6 1 2 Importing Program Files from a memory stick to the GS4M e Make sure the USB memory stick is inserted in the USB slot on the side of the instrument e Click View amp Edit Files to display your personal file folder e Click View USB to access the USB files e Highlight the desired program by clicking on it once e Click Restore Programs G Storm Thermal Cyclers USB Memory Stick Operation 43 6 1 3 Uploading Primer Sequences from a memory stick The Program Wizard function offers the option to upload primer sequences stored in an external memory stick e Click the Program Wizard button e Click Browse in the Sequence Primer One Sequence Primer Two section and browse for the required files in the Upload Sequence from USB Memory Stick screen window e Select the desired file and click Open to upload the file to the thermal cycler NOTE Primer Sequence files must have txt file format 6 2 Archiving Reports GLP and LabBook reports cannot be deleted from
44. the cycler An Administrator is authorised to archive reports to a USB Stick thus removing them from the instrument e Login as the Administrator e Insert the USB memory stick in the USB slot on the side of the instrument e Click the Reports button on the Home Page e Select the directory from which reports are to be archived Click Archive Reports this button will only be visible when a USB stick is detected e A message will appear once the archiving is complete Click OK To view and print archived reports use the PC demonstration version of G Storm software which can be downloaded from the Software page at www gstorm co uk home Insert the USB stick containing archived reports into the PC USB port Click on the Reports button and select Import Reports All of the reports on the USB stick will be uploaded into the 44 Run Operation G Storm Thermal Cyclers software To view and print a report double click on the desired report and select Print G Storm Thermal Cyclers USB Memory Stick Operation 45 7 Maintenance 7 1 Cleaning G Storm thermal cyclers are designed to require a minimum amount of maintenance by the user They can be cleaned using water or a mild laboratory cleaning agent The instrument should not come into contact with organic solvents or aggressive solutions Ensure that no liquid enters the thermal cycler For safety reasons the device must be switched off and disconnected from the power supply prior t
45. tion G Storm Thermal Cyclers The converted gradient step will then automatically use the temperature of this best performing column NOTE Resulting temperature steps may be reconverted to gradient steps any time by disabling the Conversion option Gradient steps are not available in Japan USA and Germany ED Start Cycle Starts a cycle Cycle Name Enter the name of the cycle optional Number of Cycles Enter the required number of cycles Sh End Cycle Insert this command to terminate a cycle NOTE All programs with a Start Cycle must have an End Cycle J Cools the thermal cycler down to a temperature between 4 12 C for a Store specified period of time Store Temperature Storage temperature in C The default temperature is 10 C Hold for Duration of the Store step Infinite Holds the specified temperature for an unlimited period of time G Storm Thermal Cyclers Programming 29 Use this command to record your comments about the program Script Information 4 1 2 Program Wizard The Program Wizard is a unique feature that makes it easy for first time users to write their own protocol The Program Wizard utilises biological data to generate a program Enter primer and template information e g Primer sequence or melting temperature template length and origin of DNA The Program Wizard is also an ideal tool for first shot reactions as it uses proven calculations e g for the Annea
46. ure Minimum Annealing Temperature Sets the minimum annealing temperature Hint The temperature difference is commonly between 5 C and 10 C starting with a temperature that is 2 C above the higher Primer melting temperature Example if Tm Primer 1 is 60 C and Tm Primer 2 is 54 C the resulting Touchdown will be from 62 C to 52 C G Storm Thermal Cyclers Programming 27 Elongation Temperature Sets the elongation temperature Cycles Number of required cycles for the Touchdown section Duration Duration of the temperature step in seconds Gradient Step Use this command to define settings for the gradient function The gradient will span the blocks in the direction shown by the arrows below Enter the required minimum and maximum temperatures for the gradient and duration Minimum Temperature Gradient Enter the minimum temperature of the gradient step Temperature must not be below 30 C Maximum Temperature Gradient Enter the maximum temperature of the gradient step Temperature must not exceed 80 C NOTE The maximum minimum gradient span is 30 C 1 C Hold for Duration of the Gradient step Convert Gradient Step to a standard temperature step The Gradient step can be converted easily to a temperature step after the annealing temperature optimisation experiment To do so click the checkbox and select the temperature column with the best result from your optimisation experiment 28 Run Opera
47. w detailed instrument information such as lid status remaining time and step time or GLP file name During a run it is possible to return to the Home Page and navigate the command buttons by clicking on the Home button To return to the Profile Screen click on the relevant thermal cycler button below Cycler Monitor on the Home Page You can view current program step information of all connected thermal cyclers any time during the run by clicking on the Cycler Monitor button 5 6 4 Terminating a Run To stop a program manually before it completes click the Stop button on the Profile Screen or on the Cycler Monitor screen Alternatively use the Stop Program button on the Home Page changes from Run Program during a run The manual cancel of the program will be recorded in the GLP and lab book reports 40 Run Operation G Storm Thermal Cyclers 5 7 Reports 5 7 1 Viewing Reports Depending on the assigned user level you are allowed to view and archive reports stored in the following file folders User rights File folder Action Guest users Guest folder Viewing Lab Book and GLP reports Registered users Guest folder amp Viewing Lab Book and GLP reports Personal file folder Administrator All guest and Viewing and archiving Lab Book and user folders GLP reports To view reports proceed as follows Click Reports in the Home Page This will open the Browse Generated Reports window listing the generated reports Gr
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