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N07848_ver2.4 MagMAX Express User Manual

1. OF THIS LIMITED WARRANTY STATEMENT OR TO BIND APPLIED BIOSYSTEMS TO ANY AFFIRMATION REPRESENTATION OR WARRANTY CONCERNING THE PRODUCT THAT IS NOT CONTAINED IN THIS LIMITED WARRANTY STATEMENT AND ANY SUCH MODIFICATION AFFIRMATION REPRESENTATION OR WARRANTY MADE BY ANY AGENT EMPLOYEE OR REPRESENTATIVE OF APPLIED BIOSYSTEMS WILL NOT BE BINDING ON APPLIED BIOSYSTEMS UNLESS IN A WRITING SIGNED BY AN EXECUTIVE OFFICER OF APPLIED BIOSYSTEMS THIS WARRANTY IS LIMITED TO THE BUYER OF THE PRODUCT FROM APPLIED BIOSYSTEMS AND IS NOT TRANSFERABLE Some countries or jurisdictions limit the scope of or preclude limitations or exclusion of warranties of liability such as liability for gross negligence or willful misconduct or of remedies or damages as or to the extent set forth above In such countries and jurisdictions the limitation or exclusion of warranties liability remedies or damages set forth above shall apply to the fullest extent permitted by law and shall not apply to the extent prohibited by law Damages Claims and Returns Damages If shipping damage to the product is discovered contact the shipping carrier and request inspection by a local agent Secure a written report of the findings to support any claim Do not return damaged goods to Applied Biosystems without first securing an inspection report and contacting Applied Biosystems Technical Support for a Return Authorization RA number Applied Biosystems MagMAX Exp
2. PCR PCR amplification PCR product post PCR purification primer probes processing processor purification RNA mRNA RT PCR separation separator silica coated beads silica coated microspheres silica coated particles streptavidin coated beads streptavidin coated microspheres streptavidin coated particles subtraction libraries superparamagnetic beads superparamagnetic microspheres template Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 40 Chapter 12 Glossary and Abbreviations 12 2 Literature Aasheim H C Deggerdal A Smeland E B and Hornes E 1994 A simple subtraction method for the isolation of cell specific genes using magnetic mono disperse polymer particles Biotechniques 16 4 716 721 Coche T Dewez M and Beckers M C 1994 Generation of an unlimited supply of subtracted probe using magnetic beads and PCR Nucleic Acids Res 22 1322 1323 Cudjoe K S 1999 Immunomagnetic particle based techniques Overview In Eds Robinson R K Batt C A and Patel P D Encyclopaedia of Food Microbiology Academic Press London UK Pp 1088 1094 Hornes E and Korsnes L 1990 Magnetic DNA hybridisation properties of oligonucleotide probes attached to superparamagnetic beads and their use in the isolation of poly A mRNA from eukaryotic cells Genet Anal Tech Appl GATA 7 145 150 Jakobsen K S Breivold E and Hornes E 1990 Purification of
3. and disposal 1 2 Biohazard Warning Biological samples such as tissues body fluids infectious agents and blood of humans and other animals have the potential to transmit infectious diseases Follow all applicable local state provincial and or national regulations Wear appropriate protective equipment which includes but is not limited to protective eyewear face shield clothing lab coat and gloves All work should be conducted in properly equipped facilities using the appropriate safety equipment for example physical containment devices Individuals should be trained according to applicable regulatory and company institution requirements before working with potentially infectious materials Read and follow the applicable guidelines and or regulatory requirements in the following e U S Department of Health and Human Services guidelines published in Biosafety in Microbiological and Biomedical Laboratories stock no 017 040 00547 4 http bmbl od nih gov e Occupational Safety and Health Standards Bloodborne Pathogens 29 CFR81910 1030 http www access gpo gov nara cfr waisidx_01 29cfr1910a_01 html e Your company s institution s Biosafety Program protocols for working with handling potentially infectious materials Additional information about biohazard guidelines is available at http www cdc gov Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 7 Chapter 2 About the User Manual 2 About
4. plate s is are placed onto the moving plate carrier in the correct position and the plate carrier is pushed into the end position During the operation the front and top lids can be closed Fig 4 7 or open Fig 4 2 Closed lids protect the processing against environmental contamination The operating principle employed is MPP inverse magnetic particle processing technology Fig 4 6 Rather than moving the liquids the magnetic particles are moved from microwell to microwell containing specific reagents in contrast to the external magnet method Magnetic particles are transferred with the aid of magnetic rods covered with disposable specially designed plastic tip combs Fig 4 5 MagMAX Express magnetic particle processor Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 14 Chapter 4 Functional Description 4 3 Principle of the MagMAX Express magnetic particle processor Magnetic rod uw Transfer Tip nes XXX A i Magnetic particle Collection of Magnetic particles Release of Ready suspension before magnetic particles on the tip surface magnetic particles suspension collection Fig 4 6 Inverse magnetic particle processing 4 4 Working with a magnetic rod Working with magnetic particles can be divided into five separate processes collecting particles releasing particles washing particles incubation concentra
5. 50 Advantages of processing magnetic particleS oocococcnnconononnooonnnonnnnnnannanononennnnnncnnnnn non nnn nnne nian 11 ADplICAtlOF iiti tr A bre AT aT Fees Ere Page ees 6 33 41 49 B Brief User s Guide ii fag eir a etur dta Pear Fee a E rea Petr deep ein eg i PER EE dh 48 C EFEN S EEE EE E emat A idest damit tue ta D Dese EE 20 26 39 Certificate of DECONTAMINATION sese nenn nn nnnnnnennnr ntis ss naa sas nasa dada da gan 27 34 46 Che6CK iiie ii Gta Fra ose ua hy Duos cat dae m To Bead e rca a E Eo d e EE d Lao aa 17 20 32 34 Le ETET o EAE SAEED PEE EA ii 18 25 26 33 rmiagtietic rods O 25 Processing chatmlbet itu aa a a aa aa o 25 Concentration icti re iiri rm EG Te E AT TE EPE EE ATETA AEAEE 15 16 D Decontaminatio Ma ence teet a a aaaeeeaa rae aa aaea CORR cud 27 34 46 GertiflCate Of dd Fe e Tu aei ra re ede A E EA 27 34 46 DIMENSIONS sais ead adic eee ceret ien eee erre tec A t e d ve er e a De dod 28 A O O O NA 30 DISINFECTION procedure iatea A ead a eyes Ro STER RT dave 24 25 26 27 34 Disposal of instr rmebt ioo crie cn FI e re o WI Ono ERE RE HEY TRE ons Pied RE ER EX te 27 Disposal of materials nee AAA n eec e eas Hr e E P Aa 26 Bh a a de oa E ca wate dea E aa eon mesa AI tains ida 11 23 33 40 41 E Environmental requirements recette teet ieee eevee tla levies edente e Eee eue e eee gei 18 Error message tora eee REG ARR Gu cq RR La rea Eno E A ERR CREER FERRE CURE
6. Appendix A Certificate of Instrument Decontamination Applied Biosystems an Applera Corporation Business Certificate of Instrument Decontamination This document must be completed in full and signed by the Customer a before returning an instrument or part hereinafter referred to as instrument to an Applied Biosystems Repair Center and b prior to any on site service to be performed by Applied Biosystems 1 Refer to page 2 for special instructions concerning Mass Spectrometers 2 The form must be attached to the outside of transport packaging prior to returning the instrument to an Applied Biosystems Repair Center for repair maintenance trade in or loan European customers of Thermal Cyclers should fax the form to Applied Biosystems Customer Services and enclose a copy in the shipping container 3 The form must be given to the Applied Biosystems Engineer prior to any service of an instrument that has been located within a laboratory in which biological agents hazardous chemicals or radioactive materials have been used Please note that Applied Biosystems cannot accept any instruments that may be contaminated with viable biological agents harmful quantities of hazardous chemicals or radioactive materials AB Instrument Number Serial Number AB Instrument Description Reason for return service RMA or RA Number if required Decontamination Information and Procedures 1 Biological Agents Has this instrument been exposed to viabl
7. Fig 4 1 MagMAX Express front view with lids closed ssssssssssssss eee 12 Fig 4 2 MagMAX Express front view with lids open naa 12 Fig 4 3 MagMAX Express rear ViW oinininiicciiniir rn 13 Fig 4 4 MagMAX Express internal VW 13 Fig 4 5 MagMAX Express magnetic particle Drocessor sss nennen nn 14 Fig 4 6 Inverse magnetic particle processing ssssssssssssssssasss esee nhanh nnns enn natae nnne nnns 15 Fig 4 7 Concentration step during magnetic particle processing ssssssssse ens 16 Fig 5 1 MagMAX Express transport lock tag present before release of the lock piece 19 Fig 5 2 Location of the transport lock SCrews uo ccccceeecneeneceeeeeeetecacneceneeeeeetesnenaecseeeneetesneneesseesentesnnaes 19 Fig 5 3 Connecting the mains supply cable eene dead an 20 Fig 6 1 MagMAxX Express keypad iie e i a ER e a e Rae RS 21 FIG 0 2 Loading the plate Carel i e PR E oM EE REPE RF ER EE ARDOR 22 Fig 6 3 Positioning of the plate s and insertion of the tip COMDIS ococcocccococacacononenononanononanonnoncnnnnnos 22 Fig 6 4 Tip comb specially designed for the MagMAX Express sssssss sens 23 Fig 6 5 Plate containing microstrips specially designed for the MagMAX Express sssssssss 23 Fig 16 1 MagMAX Express keypad iei iie ireira ein essei eir sistat nasse sina naeh nsns 48 Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 45 15
8. Guide e Switch the MagMAX Express on p 20 e Pipette the samples reagents and washing buffers into the microwells of the plate s p 46 e Load the plate s onto the plate carrier p 22 e Insert the tip comb s into the tip comb holder slot s p 22 e Select the desired protocol from the keypad and display p 21 e Press the START button p 22 e After the end of the run remove the plate and tip combs p 24 e Maintain your MagMAX Express instrument regularly p 25 IS Applied A Bibtystems MagMAX Express v Fig 16 1 MagMAX Express keypad Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 48 Appendix C MagMAX Express Feedback Form Please send to Applied Biosystems Fax 512 651 0201 17 Appendix C MagMAX Express Feedback Form Cat no Serial no PURCHASED BY PURCHASED FROM Company Institute Distributor Department Address Address Tel Tel Fax Date of delivery Internet home page Date of purchase Your research area Dr O Mr O Mrs O Ms O Job title Position Surname block capitals First name block capitals Internet e mail address Excellent Above As Below Comments expectations expected expectations Reagent kit Instructions O O L1 a Instrument User manual O O L1 O Operational reliability O O Oo O Design O L1 O O Ease of use L1 O O L1 Operational costs Li O O O Customer support O O O O Further instrument system developments desir
9. PCB CSU 02 1 PCB CSU 01 Power supply 1 H motor assembly M motor assembly Free wheel assembly 1 Magnetic plate assembly Chapter 12 Glossary and Abbreviations Quantity 1 50 pcs box 50 pcs box 10 units year ENABLE Contact the Applied Biosystems technical service department Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 39 Chapter 12 Glossary and Abbreviations 12 Glossary and Abbreviations Frame The frame where the special microstrips are placed Magnetic rod The rods which are magnetic and collect magnetic particles The rods do not collect the particles on their own the magnetic rods must always be protected by a tip comb Microstrip The wells where all the reagents and samples are located and where the or microwell strip processing takes place Plate Frame microstrips 12 pieces Tip comb Protects the magnetic rod A tip comb always has to be fitted into the tip comb holder slot during processing 12 1 Keywords for web pages affinity separation amplification Applied Biosystems beads cloned DNA cloning DNA DNA complex DNA immobilization DNA purification DNA strand separation DNA template double stranded DNA dsDNA encapsulated magnetic microspheres genome genomic DNA hybride selection MagMAX Express magnetic beads magnetic microspheres magnetic particles magnetic separation microspheres nucleotide oligo dT particles
10. can be potentially infectious Dispose of all used microplates AN disposable gloves syringes disposable tips etc as biohazardous waste 7 3 Decontamination procedure If there is any risk of contamination with biohazardous material the procedure recommended below or some other corresponding decontamination procedure must be performed We strongly recommend that the complete decontamination procedure is performed before relocating the instrument from one laboratory to another Decontamination is not required for the proper functioning of the instrument Example of decontaminants Ethanol 70 Virkon solution 1 3 Glutaraldehyde solution 4 Chloramine T Microcide SO 1 64 Warning Always use disposable gloves and protective clothing and operate in a well ventilated area 1 Prepare the decontaminant for example 200 ml 4 glutaraldehyde solution or another agent recommended by your safety officer 2 Empty the plate carrier Ensure that you are wearing disposable gloves 3 Switch OFF the power and disconnect the mains supply cable Fig 5 3 4 Disinfect the outside of the instrument using a cloth dampened with 70 ethanol b Place the instrument in a large plastic bag Ensure that the lids are open 6 Place a cloth soaked in the prepared solution into the bag Ensure that the cloth does not make contact with the instrument 7 Close the bag firmly and leave the instrument in the bag for at least 24 hours 8 Remove the
11. display is a 20 character by 2 row line LCD The lower line of the display can show the steps of the protocol while the processing is running Furthermore if errors occur they will be displayed on the lower row The keypad and display are shown in Fig 6 7 NS Applied AB Biosystems MagMAX Express Fig 6 1 MagMAX Express keypad The keys available are two cursor keys or arrow keys direction keys to select the processing step to be run To change to the previous next protocol by scrolling up Ww To change to the next protocol by scrolling down two function keys START To initiate the processing step STOP To pause terminate the processing step In short STOP paused START the instrument continues after a Pause step STOP paused STOP the processing is terminated Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 21 Chapter 6 Routine Operation 6 3 How to START e First of all slide the plate carrier out Fig 6 2 Fig 6 2 Loading the plate carrier e Load only maximum two special plates Fig 6 5 onto the plate carrier for processing at a time The plates have to be positioned so that the cut corners face left down Fig 6 2 They also have to be in their places Slide the plate carrier fully back in AB sem Cut corner Fig 6 3 Positioning of the plate s and insertion of the tip comb s Applied Biosystems MagMAX Express User Manual Rev 2 4 Dec
12. free Warranty Period Effective Date Any applicable warranty period under these sections begins on the earlier of the date of installation or ninety 90 days from the date of shipment for hardware and software installed by Applied Biosystems personnel For all hardware and software installed by the buyer or anyone other than Applied Biosystems and for all other products the applicable warranty period begins the date the product is delivered to the buyer Warranty Claims Warranty claims must be made within the applicable warranty period or for chemicals or other consumable products within thirty 30 days after receipt by the buyer Warranty Exceptions The above warranties do not apply to defects resulting from misuse neglect or accident including without limitation operation with incompatible solvents or samples in the system operation outside of the environmental or use specifications or not in conformance with the instructions for the instrument system software or accessories improper or inadequate maintenance by the user installation of software or interfacing or use in combination with software or products not supplied or authorized by Applied Biosystems and modification or repair of the product not authorized by Applied Biosystems Warranty Limitations THE FOREGOING PROVISIONS SET FORTH APPLIED BIOSYSTEMS SOLE AND EXCLUSIVE REPRESENTATIONS WARRANTIES AND OBLIGATIONS WITH RESPECT TO ITS PRODUCTS AND APPLIED BIOSYSTE
13. mRNA directly from crude plant tissues in 15 minutes using oligo dT microspheres Nucleic Acids Res 18 3669 Jakobsen K S Haugen M Saeboe Larsen S Hollung K Espelund M and Hornes E 1994 Direct mRNA isolation using magnetic Oligo dT Beads A protocol for all types of cell cultures animal and plant tissues In Eds Uhl n M Hornes E and Olsvik Advances in Biomagnetic Separation Eaton Publishing Pp 61 71 Lambert K N and Williamson V M 1993 DNA library construction from small amounts of RNA using paramagnetic beads and PCR Nucleic Acids Res 21 775 776 Lee Y H and Vacquier V D 1992 Reusable cDNA libraries coupled to magnetic beads Anal Biochem 206 206 207 Rudi K Kroken M Dahlberg O J Deggerdal A Jakobsen K S and Larsen F 1997 Rapid universal method to isolate PCR ready DNA using magnetic beads BioTechniques 22 506 511 Sinclair B 1998 To bead or not to bead Applications of magnetic bead technology Scientist 12 16 19 Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 41 Chapter 13 Index 13 Index A ADbtreviatiOrns 4a teet sad ecpo cis cans rav aed eria rer dais 40 About the User Manual ie inr itn itn Eae a Een b E Fa Arabia danna dla ue Kk 8 AGCCOSSOLF OGS cci ite A redi ede ara dee od ndo da eer eda ave Fd n en 17 39 Ade ioi ec caet aso ter edet ve A xax cage D E Saa cab an E Ta RR Can Ed EE Era Ra ac RD ERR Ea gda
14. the following requirements 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC 2002 96 EC Waste of Electrical and Electronic Equipment Safety performance EN 61010 1 1993 A2 1995 IEC 61010 1 1990 A1 1992 A2 1995 including CENELEC Common Modifications US and CA National differences Installation Category Overvoltage Category Il Pollution Degree 2 EN 61010 1 2001 Ed 2 EMC performance EN 50081 1 1992 Generic emission standard Residential commercial and light industry EN 50082 1 1997 Generic immunity standard Residential commercial and light industry EN 61326 1 1997 A1 1998 Product family standard A2 2001 A3 2003 Test standards Performance limits EN 55022 1998 Class B 150 kHz 1 GHz EN 61000 3 2 1995 A1 1998 Class A A2 1998 A13 1997 A14 2000 EN 61000 3 3 1995 EN 61000 4 2 1995 A1 1998 4 kV CD 8 kV AD Criteria B EN 61000 4 3 1996 A1 1998 3 V m 80 MHz 2 GHz Criteria A EN 61000 4 4 1995 1 kV Criteria B EN 61000 4 5 1995 2 kV line to ground 1 kV line to line Criteria B EN 61000 4 6 1996 3 Vims 150 kHz 80 MHz Criteria A EN 61000 4 11 1994 3096 10 ms Criteria B 60 100 ms Criteria C 100 20 ms Criteria B gt 9596 5 s Criteria C Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 30 9 Troubleshooting Guide 9 1 Error messages and warnings Error message Warning Command error Canno
15. 2000 m Temperature 5 C 40 C Mains supply fluctuations 10 from nominal Installation category overvoltage category Il according to IEC 60664 1 see Note 1 Pollution degree 2 according to IEC 60664 1 see Note 2 Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT Il which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply The pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only nonconductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation Both of these affect the dimensioning of the electrical insulation within the instrument Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 29 Chapter 8 Technical Specifications 8 3 In conformity with the requirements The MagMAX Express bears the following markings Type 700 100 240 Vac 50 60 Hz 80 VA CE mark CSA monogram C Tick The MagMAX Express conforms to
16. MAX Express users who will e Install the Applied Biosystems MagMAX Express e Perform routine runs using the Applied Biosystems MagMAX Express e Perform planned maintenance procedures 2 1 3 Assumptions This guide assumes that your Applied Biosystems MagMAX Express has been installed by an Applied Biosystems technical representative This guide also assumes that you have a working knowledge of the Windows operating system 2 1 4 Text Conventions This guide uses the following conventions e Bold text indicates user action For example Type 0 then press Enter for each of the remaining fields e talic text indicates new or important words and is also used for emphasis For example Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 8 Chapter 2 About the User Manual Before analyzing always prepare fresh matrix e A right arrow symbol gt separates successive commands you select from a drop down or shortcut menu For example Select File 2 Open gt Spot Set Right click the sample row then select View Filter gt View All Runs 2 1 5 User Attention Words Two user attention words appear in Applied Biosystems user documentation Each word implies a particular level of observation or action as described below Note Provides information that may be of interest or help but is not critical to the use of the product IMPORTANT Provides information that is necessary for proper instrume
17. MS MAKES NO OTHER WARRANTY OF ANY KIND WHATSOEVER EXPRESSED OR IMPLIED INCLUDING WITHOUT LIMITATION WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE WHETHER ARISING FROM A STATUTE OR OTHERWISE IN LAW OR FROM A COURSE OF DEALING OR USAGE OF TRADE ALL OF WHICH ARE EXPRESSLY DISCLAIMED THE REMEDIES PROVIDED HEREIN ARE THE BUYER S SOLE AND EXCLUSIVE REMEDIES WITHOUT LIMITING THE GENERALITY OF THE FOREGOING IN NO EVENT SHALL Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 36 Chapter 10 Warranty Information APPLIED BIOSYSTEMS BE LIABLE WHETHER IN CONTRACT TORT WARRANTY OR UNDER ANY STATUTE INCLUDING WITHOUT LIMITATION ANY TRADE PRACTICE UNFAIR COMPETITION OR OTHER STATUTE OF SIMILAR IMPORT OR ON ANY OTHER BASIS FOR DIRECT INDIRECT PUNITIVE INCIDENTAL MULTIPLE CONSEQUENTIAL OR SPECIAL DAMAGES SUSTAINED BY THE BUYER OR ANY OTHER PERSON OR ENTITY WHETHER OR NOT FORESEEABLE AND WHETHER OR NOT APPLIED BIOSYSTEMS IS ADVISED OF THE POSSIBILITY OF SUCH DAMAGES INCLUDING WITHOUT LIMITATION DAMAGES ARISING FROM OR RELATED TO LOSS OF USE LOSS OF DATA FAILURE OR INTERRUPTION IN THE OPERATION OF ANY EQUIPMENT OR SOFTWARE DELAY IN REPAIR OR REPLACEMENT OR FOR LOSS OF REVENUE OR PROFITS LOSS OF GOOD WILL LOSS OF BUSINESS OR OTHER FINANCIAL LOSS OR PERSONAL INJURY OR PROPERTY DAMAGE NO AGENT EMPLOYEE OR REPRESENTATIVE OF APPLIED BIOSYSTEMS HAS ANY AUTHORITY TO MODIFY THE TERMS
18. MagMAX Express User Manual Rev 2 4 BSS BioEystems Copyright 2008 Applied Biosystems All rights reserved Information in this document is subject to change without notice Applied Biosystems assumes no responsibility for any errors that may appear in this document APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT EXPRESSED OR IMPLIED INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE IN NO EVENT SHALL APPLIED BIOSYSTEMS BE LIABLE WHETHER IN CONTRACT TORT WARRANTY OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL INCIDENTAL INDIRECT PUNITIVE MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT INCLUDING BUT NOT LIMITED TO THE USE THEREOF For Research Use Only Not for use in diagnostic procedures NOTICE TO PURCHASER License Disclaimer Purchase of this product alone does not imply any license under any process instrument or other apparatus system composition reagent or kit rights under patent claims owned or otherwise controlled by Applera Corporation either expressly or by estoppel TRADEMARKS Applera Applied Biosystems and AB Design are registered trademarks and MagMAX is a trademark of Applera Corporation or its subsidiaries in the U S and or certain other countries All other trademarks are the sole property of their respective owners Part Number N07848 Rev A January 2008 Applied Biosystems M
19. PC is needed for routine use KingFisher Software can be used to create new protocols or modify existing protocols if needed PC modified protocols are then downloaded into the processor for routine use Q3 Will the magnets get weaker If so how long can they be used Can they be replaced A3 The MagMAX Express magnets are made of material that is very stable The magnetic field will not get weaker Magnets can be replaced if needed Usually there is no need to replace them but it can be done for example if they are mechanically damaged during runs for example the plate is not correctly positioned during the run Changing the magnets can be carried out by local technical service personnel O4 How strong are the magnets Can they disturb for example some sensitive equipment A4 The magnetic field is situated just around the magnetic tips so it is very local Therefore the magnetic field should not cause any problems with surrounding equipment However if you touch your floppy disk with the magnetic rod you will lose all your data Generally a normal PC screen may also detect the magnetic field with a minor distortion of the image Q5 What happens if forget to insert the tip combs into the tip comb holder and the magnetic particles stick to the magnetic rods Ab Wipe the magnetic rods with a soft cloth or tissue paper soaked in a mild detergent solution soap solution or alcohol Q6 What if magnetic particles rem
20. RR YR RET cas 8 31 F op LEM 33 Feedback Fo EEE E O E UN FE SRM prada 17 49 SIME TDI HSIEE 32 40 Frequently asked questions emet e er ER Ee ER ete EE RA ERR il e ee Lan ehe inen 33 G GIOSSaly Staind c 40 H How to clean the magnetic TOdOS oooccccccncncnnoncncnonononnnnnnanennnn cnn nn anne rre 25 How to clean the plate carrier neisheina aaiae nn nr cnn 25 How to clean the processing chamber ocinccccccnncncnononannnncnnnonnnnnnnnnn nc nn rre 25 How to disinfect the instrument sees nnn cnn cnn nono anar nn rn nr nana rra 24 25 26 34 How to ensure Stat aa 22 How to install the MagMAX Express cocoononococccoconoconnnononcnnncnncnn cnn nn nc nnrnn rra nr rre rre narra 17 29 How to maintain the inStrUMeNt onnccccnnnnnncnnnaninona naciona nana nn cnn nro nn nn nunnan nunnan annuun nara rra rr 25 How to packFfOrsService in ecce ete eid ene A AAA a ere Een edu unas od aa aee 27 34 Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 42 Chapter 13 Index How to release the transport Ockie neadan e aa aneia cnn rr 18 How to setup the instrument before you put it into operation esesseeeeeeeeeenne enne 18 How sto shut dOWLEio 2i enia n tent a 24 HOW to SWITCH Of ice ies iei e icf ene Een tdi aee ba Lv E Pa e ga 21 23 How to troubleshoot the instrument esses nennen nnne nnne nnns ka rra 32 How to Unpack etr Ee RR E PEE E a ede dette oe 17 How to use the ke
21. a aue A Re OT ova RUOTA ERREUR aa RO Cra Fa doa 39 11 1 List Of ACCESS OFGS tii eder reed eevee tates aia PR suae eR pae nx o ean ERR aps 39 11 2 List of spare Parciales 39 11 3 List of recommended spare parts sesssssesesseeeee eene nnne nnn 39 12 Glossary and Abbreviations eesesseseseeeeeeeee eene eene enne nennen nennen naar nn nr en nnne trennen nn 40 12 1 Keywords for web pages sss essen enne enne nennen enne nnne 40 12 2 Literatures oett EAS de T dE 41 13 ipe C CE 42 14 Table ot Figures iii 45 15 Appendix A Certificate of Instrument Decontaminati0N ooccccnnnncccnnnonnccnnnonncnnnnnnannnnnnannnos 46 16 Appendix B MagMAX Express Brief User s Guide ooooooccnonncccnnnocnccnccononcnonconncnnnnnnnnnncnannnos 48 17 Appendix C MagMAX Express Feedback Form esee 49 18 Appendix D Addresses uiii iia aida 50 Applied Biosystems MagMAX Express User Manual Rev 2 4 January 2008 5 Chapter 1 Safety Symbols and Markings 1 Safety Symbols and Markings These symbols are intended to draw your attention to essential information and alert you to the presence of hazards as indicated SAFETY SYMBOLS USED IN THE MagMAX EXPRESS Power ON Power OFF O Serial number RET Catalog number Date of manufacture BA Consult instructions for use WEEE symbol This product is required to comply with the European Union s Waste Electrical amp Ele
22. ackaging for future transportation The packaging is designed to assure safe transport and minimize transit damage Use of alternative packaging materials may invalidate the warranty Also retain all instrument related documentation provided by the manufacturer for future use 5 1 2 Checking delivery for completeness Check the enclosed packing list against order If any parts are missing contact your local Applied Biosystems representative or Applied Biosystems 5 1 3 Checking for damage during transport Visually inspect the transport package the instrument and the accessories for any possible transport damage If the carton has been damaged in transit it is particularly important that you retain it for inspection by the carrier in case there has also been damage to the instrument Visually check all interconnections in the basic instrument Check that there are no loose parts inside the instrument If any parts are damaged contact your local Applied Biosystems representative or Applied Biosystems 5 1 4 Environmental requirements When you set up your MagMAX Express avoid sites of operation with excess dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature fluctuations e Make sure the working area is flat dry clean and vibration proof and leave additional room for cables lids etc e Make sure the ambient air is clean and free of corrosive vapors smoke and dust e Make
23. ad and display are preloaded in the on board software MagMAX Express Fig 3 1 MagMAX Express magnetic particle processor Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 10 Chapter 3 Introduction to the MagMAX Express 3 3 Advantages of processing magnetic particles with the MagMAX Express The MagMAX Express magnetic particle processor provides several advantages relating mainly to the principle of operation in that it Enables automation of complicated manual steps Enables simultaneous processing and purification for example of double stranded DNA Enables a quicker reaction and a more efficient wash due to the simultaneous shaking during incubation and washing Facilitates a good collection of bead bound sample due to the efficiency of the magnet Prevents carry over due to the fact that the same tip is only used for one sample and furthermore that the reagents have been pipetted into their own wells different reagents are not added into the same well Is easy to carry out the whole processing with the aid of an internal program Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 11 Chapter 4 Functional Description 4 Functional Description 4 1 Instrument layout 4 1 1 Front view 1 Keypad 2 Display 3 Frontlid 4 Top lid Fig 4 1 MagMAX Express front view with lids closed Fig 4 2 MagMAX Express front view with lids open Applied Biosyst
24. agMAX Express User Manual Rev 2 4 January 2008 Contents Contents 1 Safety Symbols and Markings como iii 6 2 About the User Manual ccoo 8 3 Introduction to the MagMAX Express cccceeeeeeeeeeeeeeeeeeeeeeeeeaeeaeeeeeeeseeseaaeaeeeeeeeeeeeeaaes 10 4 Functional Description nerit sire esed ttai casesesccutandeccutensacduubneed cvduvessescouedccceuweacats 12 5 Installation 17 6 Routine Operati sins uesia oret et Dep iii 21 7 Maintenance cc lt ooccioorrnciioncirn rar ri ay SUNAN aa ONW MA AEE VEAS SUNAN aa ENVE ENUEN NA A ONAE a y SANAA AANER ANS 25 8 Technical Specifications ici ira 28 9 Tro bleshooting Guide 1 2 n etitm iere dis 31 10 Warranty Information iet rci aaea ue co Fede pe do eran A Tego so eL eaae ca a 35 11 Ordering Information ioris raton nor ici iii cien 39 12 Glossary and Abbreviations iet itas acne Rc E caia F aa ka aL Ra cd 40 13 INOX 42 14 Table of Figures e M 45 15 Appendix A Certificate of Instrument Decontamination useeeeeeeeeeeeeeeneeee 46 16 Appendix B MagMAX Express Brief User s Guide c cccccsseeeeeesseeeeeeesseeeeeesseeeeeenseees 48 17 Appendix C MagMAX Express Feedback Form eese nnne 49 18 Appendix D Addresses eei tienes deine tuned e siae deca davana D
25. age provides access to worldwide telephone and fax numbers to contact Applied Biosystems Technical Support and Sales facilities Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 9 Chapter 3 Introduction to the MagMAX Express 3 Introduction to the MagMAX Express 3 1 Intended use The MagMAX Express magnetic particle processor Fig 3 7 is intended for professional research use by trained personnel The instrument is intended for automated transfer and processing of magnetic particles in a microwell scale Use for self testing is excluded It is recommended that Good laboratory Practices GLP are followed to guarantee reliable analyses 3 2 Principle of operation The MagMAX Express magnetic particle processor Fig 3 7 is designed for the automated transfer and processing of magnetic particles in a microwell scale The principle of the MagMAX Express system patents pending is based on the use of magnetic rods covered with the disposable specially designed tip combs and microstrips These are provided with the reagent kit The instrument functions without any sucking or aspiration parts or devices Samples and reagents including magnetic particles are dispensed into the wells according to the corresponding kit instructions The use of microplate format allows the use of microwell scale volumes and existing liquid handling tools The steps of the protocol that are selected by the user via the keyp
26. ained in the sample well A6 If the starting material is too viscose the magnetic rods will not be able to collect the particles Dilute the sample and check that the sample is properly homogenized lysed Q7 What if magnetic particles are attached to the tip combs A7 This happens sometimes but it will not affect the yield because the sample has been released from the particles Q8 Are the volumes of reagents in each well critical A8 We strongly recommend that you keep the given volumes within certain limits to avoid failure in performance of the chemical reactions and the processor Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 33 Chapter 10 Warranty Information 9 4 Service request protocol If the MagMAX Express requires service contact your local Applied Biosystems representative or Applied Biosystems Do not under any circumstances send the instrument for service without any prior contact It is imperative to indicate the fault and nature of the required service This will ensure a faster return of the instrument to the customer Your local Applied Biosystems representative or distributor will take care of sending a complaint form that is the Warranty Claim Technical Sheet to the Applied Biosystems technical service department The Warranty Claim Technical Sheet contains a more detailed description of the fault symptom or condition Give all the necessary information to the distributor who
27. and a frequency range of 50 60 Hz 5 2 Setups before you put the instrument into operation 5 2 1 How to release the transport lock Note THERE IS ONE TRANSPORT LOCK PRESENT Fig 5 2 A white tag with the red text CAUTION Remove Transport Lock Before Use is fastened to the instrument to attract the user s attention Fig 5 7 The tag in Fig 5 7 and the black arrows in Fig 5 2 indicate where the transport lock piece is situated Remove the tag and release the transport lock Ensure that the transport lock has been released before you put the instrument into operation Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 18 Chapter 6 Routine Operation Fig 5 1 MagMAX Express transport lock tag present before release of the lock piece 1 To remove the transport lock unscrew the three screws Fig 5 2 with the 2 5 mm hexagonal screwdriver supplied 2 Remove the transport lock piece 3 Save the transport lock piece and screws and the transport lock warning tag for future transportation Tag attached here Fig 5 2 Location of the transport lock screws Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 19 Chapter 6 Routine Operation 5 2 2 How to ensure the startup panel is in the OFF position 2 Note Connect the mains supply cable to the mains power socket Fig 5 3 at the bottom left of the back panel If any other type of mains supply cable than s
28. ctronic Equipment WEEE Directive 2002 96 EC WARNING MARKINGS USED IN THE DOCUMENTATION Warning risk of electric shock A Warning biohazard risk Warning risk of personal injury to the operator or a safety hazard to the surrounding area OTHER MARKINGS USED IN THE DOCUMENTATION s Note marks a hint important information that is useful in the optimum 1 operation of the system or an item of interest Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 6 Chapter 1 Safety Symbols and Markings 1 1 Chemical Safety Guidelines To minimize the hazards of chemicals e Read and understand the Material Safety Data Sheets MSDSs provided by the chemical manufacturer before you store handle or work with any chemicals or hazardous materials e Minimize contact with chemicals Wear appropriate personal protective equipment when handling chemicals for example safety glasses gloves or protective clothing For additional safety guidelines consult the MSDS e Minimize the inhalation of chemicals Do not leave chemical containers open Use only with adequate ventilation for example fume hood For additional safety guidelines consult the MSDS e Check regularly for chemical leaks or spills If a leak or spill occurs follow the manufacturer s cleanup procedures as recommended in the MSDS e Comply with all local state provincial or national laws and regulations related to chemical storage handling
29. e Da dadura aeeaiei iaei 50 Applied Biosystems MagMAX Express User Manual Rev 2 4 January 2008 3 Table of Contents Table of Contents 1 Safety Symbols and Marking cccccceesscceeeeeseeceeeenceeeeeesceeeseesneaeeessseaeseeseeeeesessseaeeeesseaeenenaees 6 1 1 Chemical Safety Guidelines ooooccccnnnccccnonnnnnccnnnannncnannnnncnanonnncrnnn cnn ennemi nennen nens 7 1 2 Biohazard Waring i reU PR a Fern ee e ba fee ta 7 2 About the User Manual rene eerie rye etre ener pane erae erui gone e dera na na aaiae niai 8 AME Cir EL E 8 2 1 1 Purposes or This Guide iii erii rete Ln a de c I Lone Fa Te i EP Eon ETE FO 8 2 1 2 AudiehGe ue alee te t ert A T 8 2 1 3 ASsUumptlons A NGA REN QE CREARE P RERO RUE 8 2 1 4 Text Conventions sesssesseeeseee eene nennen nennen nnne sse nnns inen nnns seen nnns seen inen nnns nnne nn 8 2 1 5 User Attention WOrdS iii E tpe e E TR E E EE DR ROTER ead adie ka net eat 9 2 1 6 Salty Alert Wards indios coded dl Wh te dr ee e ege vd due 9 2 2 How to Obtain More Information essen nennen enne nnne rra 9 2 2 1 Related DocumentatiON ccoocccccononnnnonnncnonnnnnnnnnnnnnn ren nn anne 9 2 3 Howto Obtain SUPpoOrt eres ian r O dc e EE 9 3 Introduction to the MagMAX Express ccccseeceeceeeeeeeeeeeeeeeeeee eee nennen en nn ennt nene nn nnne nn nnn 10 3 1 la ho UE c 10 3 2 Principle of operation iori cinere e
30. e biological agents YES LH NO LH If YES please state the viable biological agent s their Hazard Group s and Biosafety Level Category of Containment If YES please describe the procedures used to decontaminate the instrument 2 Radioactive Materials Has this instrument been exposed to radioactive materials YES O NO LH If YES please identify the radioactive isotopes If YES please describe the procedures used to decontaminate the instrument If YES are the radioactivity survey results at or below local background level YES O NO O Attach the results to this form Or in the US only for service work excluding any transportation at levels designated to be clean or safe as stated in the Customer s regulatory approved Site Radioactive Materials License 3 Hazardous Chemicals Has this instrument been exposed to chemicals that are very toxic in quantities harmful to human contact carcinogenic mutagenic toxic for reproduction sensitizing and or which have not yet been fullytested YES O NO O If YES please identify the hazardous chemicals If YES please describe the procedures used to decontaminate the instrument The Customer understands and agrees that decontamination is critical to issues of health and safety and that thoroughly completing this Certificate is essential Customer represents and warrants to Applied Biosystems that it performed all decontamination procedures as described in this Certificate and completed th
31. e ce Eee PO d Pr Goes ED Ta a Tate E e E edes 10 3 3 Advantages of processing magnetic particles with the MagMAX Express 11 4 Functional Description nr Deere sustncencdniecod dashesnadedeces A a 12 4 1 Instrument layout ie dic rh EE e e ER LE Hd ee tru un 12 4 1 1 Eront VIOW t tet tere rure dett ed ee eA TH ee date e dept cedet 12 4 1 2 Back Vida 13 4 1 3 Internal VIEW isi iiinn 13 4 2 MagMAX Express magnetic particle processor sse 14 4 3 Principle of the MagMAX Express magnetic particle processor eeeeeeees 15 4 4 Working with a magnetic rod ssssssesssssssseseesesee eene nennen nnne n nnn nr terree nnn nnne nenas 15 4 4 1 Collecting magnetic particles seseesseeeeeeeee ener 15 4 4 2 Releasing magnetic particles ssssssssssesseeseseeeen nennen enne nennen trennen nnn 15 4 4 3 Washing magnetic particles eessssssssssssesseeeeene nennen ennt nnne nnns 16 4 4 4 IiGubati n sa er tme Dn RE E Rea EI 16 4 4 5 Changing the volume during the magnetic particle processing sssss 16 DD E AA HE 17 5 1 What to do upon delivery esssssssssseesesee esee nennen eren nnne r ana Kapea iana Eas 17 5 1 1 How to unpack hcic Ee hd te teen ce rper ER e ER TR Ren T 17 5 1 2 Checking delivery for completeness eseesseeese
32. e ic LE a EFE ao nda d ama EY er Eva Fa Tra Ede To Ra EVA SY Oa ro ATA XA TEA Pa n aS 15 WAS Ko MARTEN 15 16 Magneti rod MELLE 10 13 14 15 16 25 28 32 33 40 MaS td iaa 13 20 26 28 29 DUET AIET aE OPC EOI eoe d enn bank doen eh Sn end Freenet dais Fa rf i ec n d ee Ra bx Oa dane dea tice 25 27 48 LAETI TRES TAPER 8 25 27 34 MiGrOStEIz ierit eere agite le e RO EE GER AER ARTE codsdveusvenenee 10 14 23 28 32 40 O Operational checa A edit o Fe De erties teats 20 Ordering infOrtmatiOn c cen incineradora Rap kao dada aya La n EE EP e aa ARO IRE Ea aaa as 8 39 P PACKING as a dd da 17 27 34 SUCIA ada do 17 27 A A A a 17 POR ua rada iris nri dem tetra uir dh A Bos id ta acaba 33 40 41 Plate icti a e Cae da 10 13 14 22 23 24 25 26 32 33 39 40 48 CMA A T O tada io 14 22 24 25 26 48 Plate Care ti A A epe uei dad tuna lara EE vu ovas 14 22 24 25 26 48 Processing head iret t RERO RE ti 14 Protocol cnet tete oa et E te e Tee Pre t Ede A bee Pa Ede Edu 10 21 33 34 41 48 R Releasing the transport lock tte EBEN aaea EE ERREUR a 18 A A A E EES T E E ASAA EAE EEE AE AA e AE E E E OTT 40 41 aA a O n ca a E A EE E E EEA E EE EEE E AE 40 Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 43 Chapter 13 Index S SAM Ple PP EIER 10 11 14 18 23 26 28 33 40 48 I Iais m S TRI 27 28 32 33 34 COMMER ei T EE E E E E E E N E 27 PACK foran S 27 34 reques
33. e painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur Clean the display areas with a mild laboratory detergent The keypad has a wipe clean surface Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol Warning If any surfaces have been contaminated with biohazardous material a mild MEN sterilizing solution should be used Note Do not autoclave any part of this instrument 7 1 1 How to clean the plate carrier Keep the plate carrier surface clean to avoid dust and dirt entering into the instrument Clean the plate carrier surface at least once a week using a soft cloth or tissue paper soaked in a mild detergent solution SDS soap solution or alcohol If you have spilt infectious agents on the plate carrier disinfect according to Section 7 3 Decontamination procedure 7 1 2 How to clean the magnetic rods If required clean the magnetic rods using a soft cloth or tissue paper soaked in a mild detergent solution SDS soap solution or alcohol Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 25 Chapter 7 Maintenance 7 1 3 How to clean the processing chamber If required clean the processing chamber using a soft cloth or tissue paper soaked in a mild detergent solution SDS soap solution or alcohol 7 2 Disposal of materials Refer to local regulations for the disposal of infectious material Warning The samples
34. e suspension evenly mixed in long running reactions the tip comb s can be moved up and down from time to time 4 4 5 Changing the volume during the magnetic particle processing The volume of the first microwell can be larger than the volume of the next microwell and this is used for concentration purposes see Fig 4 7 below Y Transfer r v A y E gt El N j 1 v v j o rd Collection of Transfer into Release of Ready magnetic particles a low volume well magnetic particles suspension in a from a large volume low volume well well Fig 4 7 Concentration step during magnetic particle processing Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 16 Chapter 6 Routine Operation 5 Installation 5 1 What to do upon delivery 5 1 1 How to unpack Move the unpacked instrument to its site of operation Unpack the MagMAX Express instrument and accessories carefully with the arrows on the transport package pointing upwards The following notes and instructions are sent with the instrument and are immediately available when you open the package packing instructions packing list MagMAX Express Feedback Form Warranty Certificate card MagMAX Express User Manual Note Do not touch or loosen any screws or parts other than those specially 1 designated in the instructions Doing so might cause misalignment and will void the instrument warranty Retain the original p
35. ed Further applications desired Where did you first learn about the product Would you like to receive information about other Applied Biosystems products Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 49 18 Appendix D Addresses For the latest information on products and services visit our website at http www appliedbiosystems com Manufactured by Distributed by Thermo Fisher Scientific Oy Applied Biosystems Ratastie 2 P O Box 100 850 Lincoln Center Drive FI 01621 Vantaa Foster City CA Finland 94404 USA Tel 358 9 329 100 Fax 358 9 3291 0415 Tel 650 570 6667 www thermo com 800 874 9868 Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 50
36. eeeeeee nennen nennen nnns 17 5 1 3 Checking for damage during transport onoccccconoccncnaninnncnaninnncnanannnccnna cnn nrnnna nn nrrnnannncnnna ns 17 5 1 4 Environmental requirements sess eene nnne nennen nnne erre 17 5 1 5 TINGS O VOI n E 18 5 1 6 Technical prerequisites ti rrt e RE ER e REIR EE e ERE EE REA TRE 18 5 2 Setups before you put the instrument into operation ooooccconccnnncnonnnncnananoncnanannncrannnnnnnnnn nn 18 5 2 1 How to release the transport lock sess 18 5 2 2 How to ensure the StaltUPD cccnnnnncccnnnncnnnnnnnnnnonnnnnnnnnnnnnn ne nn nnn nn nennen internen nne Te nnne nnne ttr nen nnn 20 5 3 Operational Check enmaai heri 20 6 Routine Operation ia 21 GU OWING Rel aa 21 6 2 How to use the keypad and diSplaY oooonccocccnnnnnninanoconccnnnnnnannnannnccnnnnn cnn anne nn cnn rre 21 6 3 Howto STAR Tita dt Cheer vae sr oed eL ted idee a scr ek e ge e EE dads 22 6 4 Sh tdOWhs eire rdi ce sang cod n ane era vena NOR a dix en aO ER ane RERBA XRa REM x 24 Applied Biosystems MagMAX Express User Manual Rev 2 4 January 2008 4 Table of Contents 7 Maintenance wisit sececiecciccsadasccueness deeeececcueensecdvuscasceuennes dovaesdencuien secduvessueduventacduvaucccdvessaccuvoscucdvueeiacens 25 7 1 Regular and preventive maintenance ssesssssssssseeseseee teeta nennen enne nnne nnns 25 7 1 1 How to clean the plate car
37. ember 2008 22 Chapter 6 Routine Operation e The tip comb s always has have to be in its their slot s and fully inserted Fig 6 3 The instrument also functions with either one microplate or one to twelve microstrips depending on the amount of samples At least one tip comb Fig 6 4 always has to be inserted into either of the narrow slots meant for the tip comb Fig 6 4 Tip comb specially designed for the MagMAX Express e Close the front lid if you want to The front lid protects the instrument against environmental contamination Note The front lid and or top lid can be left open if desired This action does not break 1 the run e Select the name of the method for example Genomic DNA 1 e Use the START key to initiate the run For startup refer to Section 6 1 Switching on Note The samples are collected immediately after the run and transferred into other 1 tubes for longer storage 4 qp tt 4 ote 4224 Fig 6 5 Plate containing microstrips specially designed for the MagMAX Express Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 23 Chapter 6 Routine Operation 6 4 Shutdown Switch the MagMAX Express off by pressing the power switch Fig 4 3 at the bottom left of the back panel of the instrument into the OFF position Warning Remove any plate still on the plate carrier Dispose of all microplates as A biohazardous waste Warning Remove any tip comb
38. ems MagMAX Express User Manual Rev 2 4 December 2008 12 Chapter 4 Functional Description 4 1 2 Back view ON OFF switch Mains power supply socket Cooling air outlet Serial port RS 232C hoUONnN Fig 4 3 MagMAX Express rear view 4 1 3 Internal view ea iiti Plate carrier Tip comb holder Tip comb slot Fixed magnetic rod holder hWwWD Fig 4 4 MagMAX Express internal view Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 13 Chapter 4 Functional Description 4 2 MagMAX Express magnetic particle processor The MagMAX Express magnetic particle processor Fig 4 5 is designed for maximum two 96 well plates and special microstrips which are compatible with the tip comb The plate s is are kept stationary and the only moving assembly is the processing head with tip combs and magnetic rods The head consists of two vertically moving platforms One is needed for the magnetic rods 2 x 12 pieces and the other one for the plastic tip combs Fig 4 4 One plate contains twelve separate microstrips and one sample processing uses one microstrip containing eight wells One tip comb containing twelve tips is used for processing twelve samples at a time within one plate Before starting the magnetic particle processing via the keypad and display the samples and reagents are dispensed into the wells and the tip comb s is are loaded into its their slot s The
39. instrument from the bag 9 Clean the instrument using a mild detergent Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 26 10 11 Chapter 7 Maintenance Remove any stains using 7096 ethanol After performing this decontamination procedure enclose a signed and dated Certificate of Decontamination both inside the transport package and attached to the outside of the package 7 4 How to pack for service When you ship the instrument for service remember to Inform about the use of hazardous materials Decontaminate the instrument beforehand Install the transport lock Pack the instrument according to the enclosed packing instructions Use the original packaging to ensure that no damage will occur to the instrument during shipping Any damage will incur additional labor charges Enclose a dated and signed Certificate of Instrument Decontamination see Appendix A both inside and attached to the outside of the package in which you return your instrument or other items Enclose the return authorization number RGA given by the Applied Biosystems representative Indicate the fault after you have been in touch with your local Applied Biosystems representative or Applied Biosystems See Section 8 1 for details on storage and transportation temperatures 7 5 Service contracts We recommend you maintain and service the instrument regularly every twelve months on a contract basis by the manufactu
40. is Certificate accurately truthfully and in full Customer hereby assumes all responsibility and liability for and shall defend and indemnify Applied Biosystems against injury or damage of whatever kind incurred by Applied Biosystems its employees contractors and or agents that result directly or indirectly from Customer s breach of this representation and warranty The Customer accepts that Applied Biosystems has no obligation to repair service or transport any product if this Certificate is not completed in full Name Title Company Phone Signature Date Page 1 of 2 AB Part No 4343055 rev D Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 46 Appendix A Certificate of Instrument Decontamination Applied Bibsystems an Applera Corporation Business Examples of Instrument Decontamination Procedures 1 Biological Agents The following procedures are based on widely used industry standards for instrument or part hereinafter referred to as instrument decontamination but the Customer must assess the suitability of these methods for the biological agents concerned Some instruments may require decontamination by an outside service refer to your User Manual or Applied Biosystems Representative for further information Hazard Group 1 or 2 Instruments exposed to biological agents in Hazard Group 1 or 2 situated in a Biosafety Level Containment Level 1 or 2 laboratory or exposed to any human source mate
41. nd or microstrip s is are not in place e Last release poor Plate s and or microstrip s is are not in place Check that there is nothing preventing the head from moving up and down Check that there is nothing preventing the track from moving left or right Check that there is nothing preventing the track from moving left or right Check that there is nothing preventing the magnetic rods from moving up and down Ensure that the tip comb is fully in Check that there is nothing preventing the magnetic rods from moving up and down Contact authorized service Position the plate s correctly Push the microstrip s fully into the frame Position the plate s correctly Push the microstrip s fully into the frame Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 32 Chapter 10 Warranty Information 9 3 Frequently asked questions FAQ about the MagMAX Express Q1 What does the MagMAX Express system do A1 Applied Biosystems now offers a complete MagMAX Express system the magnetic particle processor for the purification and processing of DNA RNA proteins and cells The processor handles particles automatically according to the preloaded purification protocols For more information on these applications contact Applied Biosystems Q2 Do I need a PC to control the processor A2 Normally the protocol s is are preloaded by Applied Biosystems and no
42. nt operation accurate chemistry kit use or safe use of a chemical Examples of the user attention words appear below Note The Calibrate function is also available in the Control Console IMPORTANT To verify your client connection to the database you need a valid user ID and password 2 1 6 Safety Alert Words Safety alert words also appear in user documentation For more information see Safety Symbols and Markings on page 6 2 2 How to Obtain More Information 2 2 1 Related Documentation The following related documents are shipped with the system e Applied Biosystems MagMAX Express User Guide Describes the Applied Biosystems MagMAX Express hardware and software and provides information on preparing maintaining and troubleshooting the system e Applied Biosystems MagMAX Express Service Guide Provides explanatory procedures for servicing the Magnetic Particle Processors 2 3 How to Obtain Support For the latest services and support information for all locations go to http www appliedbiosystems com then click the link for Support At the Support page you can e Search through frequently asked questions FAQs e Submit a question directly to Technical Support e Order Applied Biosystems user documents MSDSs certificates of analysis and other related documents e Download PDF documents e Obtain information about customer training e Download software updates and patches In addition the Support p
43. packed in transport packaging 25 C 50 C packed in transport packaging 100 240 Vac 50 60 Hz nominal Automatic voltage detection 80 VA max 50 VA standby 273 BTU max Serial RS 232C port Baud rate 9600 Character format 1 start bit 8 data bits 1 stop bit no parity Flow control XON XOFF Performance specifications Microplate type disposable Magnetic rods fixed Capacity Vessel volumes Vessel type disposable Tip comb disposable Magnetic particle size Collecting efficiency Incubating temperature Keyboard Display Software Special design 8 x 12 grid format 2 x 12 format 24 samples run 2 x 12 20 200 ul Special microstrip 1 x 8 wells Special design 1 x 12 format ca gt 1 um 99 vessel washing buffer Ambient temperature i e no incubation START STOP two cursor keys LCD 1 8 fixed internal protocols loading through the external PC KingFisher Software can be used to create and modify protocols Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 28 Chapter 10 Warranty Information 8 2 Safety specifications The MagMAX Express fulfills the following requirements EN 61010 1 1993 A2 1995 IEC 61010 1 1990 A1 1992 A2 1995 The safety specifications are also met under the following environmental conditions in addition or in excess to those stated in the operating conditions Altitude Up to
44. red to the standard configuration prior to providing service or technical support For systems that have built in computers or processing units installing unauthorized hardware or software may void the Warranty or Service Plan Limited Product Warranty Limited Warranty Applied Biosystems warrants that all standard components of its Applied Biosystems MagMAX Express Magnetic Particle Processor will be free of defects in materials and workmanship for a period of one 1 year from the date the warranty period begins Applied Biosystems will repair or replace at its discretion all defective components during this warranty period After this warranty period repairs and replacement components may be purchased from Applied Biosystems at its published rates Applied Biosystems also provides service agreements for post warranty coverage Applied Biosystems reserves the right to use new repaired or refurbished instruments or components for warranty and post warranty service agreement replacements Repair or replacement of products or components that are under warranty does not extend the original warranty period Applied Biosystems warrants that all optional accessories supplied with its Applied Biosystems MagMAX Express Magnetic Particle Processor such as peripherals printers and special monitors will be free of defects in materials and workmanship for a period of ninety 90 days from the date the warranty begins Applied Biosystems will
45. repair or replace at its discretion defective accessories during this warranty period After this warranty period Applied Biosystems will pass on to the buyer to the extent that it is permitted to do so the warranty of the original manufacturer for such accessories With the exception of consumable and maintenance items replaceable products or components used on or in the instrument are themselves warranted to be free of defects in materials and workmanship for a period of ninety 90 days Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 35 Chapter 10 Warranty Information Applied Biosystems warrants that chemicals and other consumable products will be free of defects in materials and workmanship when received by the buyer but not thereafter unless otherwise specified in documentation accompanying the product Applied Biosystems warrants that for a period of ninety 90 days from the date the warranty period begins the tapes diskettes or other media bearing the operating software of the product if any will be free of defects in materials and workmanship under normal use If there is a defect in the media covered by the above warranty and the media is returned to Applied Biosystems within the ninety 90 day warranty period Applied Biosystems will replace the defective media Applied Biosystems does not warrant that the operation of the instrument or its operating software will be uninterrupted or error
46. rer Survey the instrument with an appropriate radioactivity measuring instrument e g Geiger Counter or scintillation counter Satisfactory decontamination is defined as survey results at or below background level or in the US only for service work excluding transportation levels designated to be clean or safe as stated in the Customer s regulatory approved Site Radioactive Materials License Repeat steps a and b until satisfactory survey results are achieved and attach the results to this form 3 Hazardous Chemicals a Areas exposed to hazardous chemicals should be washed with an acceptable solvent such as ethyl alcohol or isopropyl alcohol b Rinse with detergent and water Mass Spectrometer Mass Spectrometers cannot be decontaminated at the customer s site Complete disassembly is required For PUMPS only a Drain any oils or other fluids and vent purge all gasses b Clean the outside of pump using the procedures described above c Seal all openings and securely package the pump to prevent damage Please note that Applied Biosystems cannot accept any instruments or parts that may be contaminated with viable biological agents harmful quantities of hazardous chemicals or radioactive materials Page 2 of 2 AB Part No 4343055 rev D Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 47 Appendix B MagMAX Express Brief User s Guide 16 Appendix B MagMAX Express Brief User s
47. rer s trained service engineers This will ensure that the product is properly maintained and gives trouble free service Contact the Applied Biosystems technical service department for more details 7 6 Disposal of the instrument e Warning Decontaminate the instrument prior to disposal See Section 7 3 and Appendix A Certificate of Instrument Decontamination on decontamination e WEEE symbol Do not dispose of this product as unsorted municipal waste Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of waste electrical and electronic equipment WEEE e European Union customers Call your local Applied Biosystems Customer Service office for equipment pick up and recycling See http www appliedbiosystems com for a list of customer service offices in the European Union e For more information contact your local Applied Biosystems representative Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 27 8 Technical Specifi Chapter 8 Technical Specifications cations 8 1 General specifications Technical specifications Overall dimensions Weight Operating conditions indoor use Transportation conditions Storage conditions Mains power supply Power consumption Heat dissipation Computer interface W x D x H 290 x 290 x 310 mm 11 4 x 11 4 x 12 2 in ca 10 kg 23 Ibs 10 C 40 C RH 90 max 40 C 70 C
48. ress User Manual Rev 2 4 December 2008 37 Chapter 10 Warranty Information Claims After a damage inspection report is received by Applied Biosystems Applied Biosystems will process the claim unless other instructions are provided Returns Do not return any material without prior notification and authorization If for any reason it becomes necessary to return material to Applied Biosystems contact Applied Biosystems Technical Support or your nearest Applied Biosystems subsidiary or distributor for a return authorization RA number and forwarding address Place the RA number in a prominent location on the outside of the shipping container and return the material to the address designated by the Applied Biosystems representative Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 38 11 Ordering Information Code 4387987 Instrument System MagMAX Express 100 240 V 11 1 List of accessories Code N07848 4388452 4388474 Item MagMAX Express User Manual MagMAX Express Tip Comb MagMAX Express Plate 200 11 2 List of spare parts Code 11272711 2305290 5186980 Item Quantity Plate tray 1 Serial cable F9 F25 for RS 232C port 1 KingFisher Software 1 11 3 List of recommended spare parts Code 1091910 1092250 2004460 2004470 2004480 21040811 21040820 21040850 24070740 24072290 Item 1 2 unit s year Tooth belt T2 5 330 Tooth belt T2 5 265 PCB PSP 01
49. rial or cell culture including clinical samples a Follow Universal Precautions and wear all applicable personal protective equipment Place the instrument in a well ventilated area b Apply a freshly made solution of 1 10 v v dilution of 5 2596 sodium hypochlorite e g Clorox bleach with water to accessible product surfaces A spray dispenser may help to ensure complete coverage C Keep surfaces wet for at least 15 minutes then wipe dry d Repeat steps b and c e Clean all decontaminated surfaces with water to remove residual bleach and wipe dry If an alternative to 1 10 v v dilution of bleach is used for instrument decontamination please provide details in written form and attached to the Certificate of Instrument Decontamination Hazard Group 3 or 4 Instruments situated in Biosafety Level Containment Level 3 or 4 laboratories must be decontaminated by the customer using an internationally approved sterilization procedure The customer must then move the instrument to either a Containment Level 1 or 2 laboratory for service Applied Biosystems employees are not permitted to enter Biosafety Level Containment Level 3 or 4 laboratories It may not be possible for Applied Biosystems to service or transport these instruments 2 Radioactive Materials Apply an industry standard radioactivity decontaminant e g Radiacwash Rad Con or equivalent to the instrument and wipe surfaces as directed by the decontaminant manufactu
50. rier nennen nnnm nnne nnne 25 7 1 2 How to clean the magnetic TOUS ccccccccononononcnnnonnnonnnnnnnnnnncnn cnn nnn nn nn rre aa ae ee nennen 25 7 1 3 How to clean the processing chamber sss enne enne 26 7 2 Disposal of materials iii tet redet iie ERE ARR ex TER CREER EET EEES 26 7 3 Decontamination procedure ssssssssssssseesee eene nennen eee nnne tr sees einn nnne nennen nen 26 7 4 Howto pack for service eet da een a Ri RC en a 27 7 5 Service CORtFacts ciue exer Aa doo 27 7 6 Disposal of the instrument sseesssssesesseeeee rai enne nennen nnne nennen nennen nnns 27 8 Technical Specifications ooooocccnnnncconnnoccconnconnnnnnnonncnnnnnnnncnnnnnnn nr rra nr rrnn nnne nennt ener nnne nennt een nnns 28 8 1 General specifications mimica dd EY ndun 28 8 2 Satety specifICatioris ei eee ia RD ER C Reeve di ir Ee a aten 29 8 3 In conformity with the requirements seesseeeseeee ener 30 9 Troubleshooting Guide ununi eiie ii 31 9 1 Error messages and warnings sssssssssssseseeee enne rre 31 9 2 Troubleshooting guide e REL es ee 32 9 3 Frequently asked questions FAQ about the MagMAX Express eene 33 9 4 Service request protocol sss ener nennen enirn nennen enirn nns 34 10 Warranty Informiation 2 rettet erei eee re pce esee a 35 11 Ordering Information ici din lio PAANAN L
51. s still in the instrument Dispose of tip combs as A biohazardous waste Wipe the plate carrier surface and the neighboring instrument surface with a soft cloth or tissue paper moistened with distilled water a mild detergent SDS sodium dodecyl sulfate or soap solution If you have spilt infectious agents on the plate carrier disinfect with 70 alcohol or some other disinfectant see Section 7 3 Decontamination procedure Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 24 Chapter 7 Maintenance 7 Maintenance 7 1 Regular and preventive maintenance For reliable daily operation keep the instrument free of dust and liquid spills Abrasive cleaning agents are not recommended because they are likely to damage the paint finish We recommend you clean the case of the instrument periodically to maintain its good appearance A soft cloth dampened in a warm mild detergent solution will be sufficient Clean the outside of the instrument and the plate carrier with clean low pressure compressed air or a cloth dampened with water or a mild detergent when necessary Although the MagMAX Express is constructed from high quality materials you must immediately wipe away spilt saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage Note Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer Do not expos
52. sure the ambient temperature range is between 10 C 50 F and 40 C 104 F Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 17 Chapter 6 Routine Operation e Make sure relative humidity is between 10 and 90 non condensing Leave sufficient space at least 10 cm at both sides and at the back of the unit to allow adequate air circulation The MagMAX Express does not produce operating noise at a level that would be harmful No sound level measurements are required after installation Note Do not operate the instrument in an environment where potentially damaging 1 liquids or gases are present 5 1 5 Things to avoid Do not smoke eat or drink while using the MagMAX Express Wash your hands thoroughly after handling test fluids Observe normal laboratory procedures for handling potentially dangerous samples Use proper protective clothing Use disposable gloves Be sure the working area is well ventilated Never spill fluids in or on the equipment pacemaker or metallic prostheses should not use this product A pacemaker or prostheses may be affected or damaged if it comes in very close contact with a strong magnetic field Warning This product contains strong permanent magnets People wearing a 5 1 6 Technical prerequisites Place the instrument on a normal laboratory bench The net weight of the unit is approx 10 kg 23 Ibs The instrument operates at voltages of 100 240 Vac
53. t move track Head position error Head timeout Track position error Track timeout Magnets pos error Magnets timeout Serial number error Serialno already set Too high pos offset Invalid parameter Out of memory No acceleration tbl NV parameters lost Progmem full Time already expired Chapter 10 Warranty Information Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 31 9 2 Troubleshooting guide Chapter 10 Warranty Information Error Cause Action e Cannot move The track cannot be moved Press STOP If the fault cannot be remedied track because the head is not up then switch the power on and off e Head position The calculated head position does Check that there is nothing preventing the error not agree with the head home head from moving up and down sensor signal e Head timeout The head has not arrived at the home sensor within the allowed time e Track position The calculated track position does error not agree with the track home sensor signal e Track timeout The track has not arrived at the home sensor within the allowed time e Magnets position The calculated magnetic rod error position does not agree with the rod home sensor signal e Magnets timeout The magnetic rods have not arrived at the home sensor within the allowed time e Nonvolatile The nonvolatile parameters of the parameters lost instrument are lost e Clattering noise Plate s a
54. the User Manual This user manual has been written for the actual end user for example laboratory technician and provides information on the MagMAX Express magnetic particle processor including installation and operating instructions Read the manual in its entirety prior to operating the instrument This user manual has been designed to give you the information you need to e Install the MagMAX Express e Use the MagMAX Express in routine the processing step e Perform basic maintenance procedures This user manual also describes all the features and specifications of the MagMAX Express hardware and on board In Chapter 9 Troubleshooting Guide you will find explanations of all error messages and a problem solving guide The user should be familiar with the contents of Chapter 7 on maintenance For warranty and ordering information refer to Chapters 10 Warranty and 11 Ordering Information In an effort to produce useful and appropriate documentation we appreciate your comments on this document to your local Applied Biosystems representative 2 1 Preface 2 1 1 Purpose of This Guide The Applied Biosystems MagMAX Express User Guide provides reference material related to the Applied Biosystems MagMAX Express Magnetic Particle Processor It describes the system theory of operation and how to prepare maintain and troubleshoot the system 2 1 2 Audience This guide is intended for novice and experienced Mag
55. tion 4 4 1 Collecting magnetic particles During the collection of the magnetic particles the magnetic rod bar is fully inside the tip The magnetic rods together with the tip comb s move slowly up and down in the microwells and the magnetic particles are collected onto the wall of the tips The magnetic rods together with the tip comb s having collected the magnetic particles can be lifted out of the microwells and transferred into the next microwells 4 4 2 Releasing magnetic particles After collection of the magnetic particles the magnetic rods together with the tip comb s are lifted from the microwells the magnetic rods are lifted off and the tip comb s is are lowered into the next microwells containing a reagent Magnetic particles are released by moving the tip comb s up and down several times with considerably high speed until all the particles have been mixed with the substance in the next reaction Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 15 Chapter 4 Functional Description 4 4 3 Washing magnetic particles Washing the magnetic particles is a frequent and an important processing phase Washing is a combination of the release and collection processes in a microwell filled with washing solution To maximize washing efficiency the magnetic rods together with the tip comb s are designed to have minimized liquid carrying properties 4 4 4 Incubation To keep the magnetic particl
56. tprotocol 45 icto ine i rd t et etit ee pet deeem 34 Sh tdoWTr i5 oiii e teer ecc EE LUE E eec EP E Oeo RE OE id 24 Ije Tel ife M o MEER 8 28 29 general uie EIU IIa ra 28 NE 28 MN a E 29 Stet TEE 20 21 23 Streptavidin coated magnetic particles oooocccconoccccnanocnncnanoncnnnnnonncnnnnno nn nr eene enne 40 SVIMDOIS MEDIDAS EP 6 T Table of Figure a isa 45 Ip COR A A a dat 10 13 14 15 16 22 23 24 28 32 33 40 48 Tr adas 17 18 19 27 28 CAI AG Cate dose A P A AE O 17 sol dec 18 19 27 packages a eiu ea Dee d reseed E e e Eee 17 27 TROUBLESHOOTING ERE OU 8 31 32 34 elec EE 32 U Unpack ii C nen Qm adimi ad oce 17 User miantal zdd ets tio etae di eo ned or eere d er ed voee e oed a rl o dn a eda ad dU OO 8 17 39 49 V MI a ia 12 13 TFOD n tee mete reati cate tee dle Reel d n debe te et a V et C e bh ei LL Cb 12 internal n Nene iia da Tr d dd P E CA REED I NER DE 13 FOAM pm M 13 Me To e a aaa 18 20 21 28 30 SS A RR NEC TERT TITRE NETTE 20 21 W Warning Sisian 18 19 20 31 luchas tes 8 17 35 Weight i en eee e TE C CREDE a e Qa QU E x ER DU EV Rv D do annie cde 18 28 Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 44 Chapter 14 Table of Figures 14 Table of Figures Fig 3 1 MagMAX Express magnetic particle Drocessor ssssssssssssse seen nnn 70
57. upplied is needed use only cables certified by the local authorities Before you plug in the power cable ensure that the voltage on the rating label at the bottom left of the back panel Fig 4 3 corresponds to the local voltage 1 Warning Ensure that the mains switch Fig 4 3 on the bottom left of the back Connect the instrument to a correctly installed line power outlet which has a protective conductor that is grounded Warning Never operate your instrument from a power outlet that has no ground connection Fig 5 3 Connecting the mains supply cable 5 3 Operational check 1 First switch the instrument ON The instrument performs initialization tests and adjustments The initialization tests are so called self tests 2 The display quickly shows the internal software version and after that the display shows one of the protocols in the internal memory This happens when the initialization tests and adjustments have been completed 3 We recommend that you carry out an empty run for example a demonstration to verify proper instrument operation Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 20 Chapter 6 Routine Operation 6 Routine Operation 6 1 Switching on Before you switch on the MagMAX Express ensure that the voltage on the rating label at the bottom left of the back panel Fig 4 3 corresponds to the local voltage 6 2 How to use the keypad and display The liquid crystal
58. will fill out and forward the Warranty Claim Technical Sheet to the Applied Biosystems technical service department Check Section 7 4 How to pack for service You will find instructions on how to proceed before shipping the instrument for service to Applied Biosystems Check that any necessary decontamination procedure has been carried out before packing Refer also to Section 7 3 Decontamination procedure Ensure that the Certificate of Instrument Decontamination see Appendix A as well as the return authorization number RGA are sent with the instrument The Applied Biosystems technical service department will keep you up to date with the progress of service and provide you with any further details you might need for example on maintenance serviceability troubleshooting and replacement Applied Biosystems MagMAX Express User Manual Rev 2 4 December 2008 34 Chapter 10 Warranty Information 10 Warranty Information Instrument Warranty Information Computer Configuration Applied Biosystems supplies or recommends certain configurations of computer hardware software and peripherals for use with its instrumentation Applied Biosystems reserves the right to decline support for or impose extra charges for supporting nonstandard computer configurations or components that have not been supplied or recommended by Applied Biosystems Applied Biosystems also reserves the right to require that computer hardware and software be resto
59. ypad and display coooooconccoccnccnnnnononononnnnnnnnnnnnnnnnn nn rn cnn rana 21 How to work with a magnetic rod cccececcccccecececececececececececececececececauaeauaeaeaeaeaeauauaeaeauaeaeaesaaanauaaaaeasaeaes 15 Incubationi 5 ite tent ao a Sudden ER ado Con er ue Ql LER Ud 11 15 16 28 IristallatiOnt nto iie dr ree te exis een Pan pest re ee avr anda Pee a added 8 17 18 27 29 Instrument Iayout nee eeepc tee A eat ath see oos 12 TENdencias 10 Inverse magnetic particle processing ocooomocccoccnnninnnonanonnncnnnnnnnnnnnn cnn nn nn cnn rana nnne nnn nnn nnne enn 14 15 K Keypad atid display 5 adeo e Re ERE cps ae eee 10 14 21 48 IOV CHEER RT MR EM ao 21 28 Keywords for web pages nt e n EP RERO HER Pi DIR HERE OI 40 Kite tei ete e cea e dee i de a ett ata ree eder E wal e gera he curte a li 10 49 L Liquid crystal display irt RO ERROREM Ie ER eile 21 Literature A eod vise ter tod cats eae ddd cvi rre eb cd eer Eel edd va iy a d e mere vts rd Ida dota E 41 M MagMAX Express 8 10 11 12 13 14 15 17 18 19 21 23 24 25 29 30 33 34 39 46 48 49 Magnetic particle DrOCesSsOr cccccccccsescececececececesececesecesecececesauaeauaeauseaeauanaeanananaes 8 10 11 14 15 33 Magnetic particles cec cernes a ii dada 10 11 14 15 16 33 40 Collection Of e CO Dac E crat iter la dad coved eva Pet ag obra deep axa Ewa pa Prev aka i dea cu x FE Cai 15 release of cnt iiie di eain Ih Ir apr

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