H301-UP - Okolab
Contents
1. 6 Figure 14 How to fix the H301 UP on the stage www oko lab com 12. H301 UP User Manual November 2014 H301 UP Compatible with the following XY stages Compatible with the following Okolab Controllers XY stage model and serial number must be H301 T BL PLUS specified in purchase order UNO COMBINED CONTROLLER H401 T DUAL Page 1 www oko lab com H301 UP User Manual November 2014 Index 1 Components and GIMENSIONS EEN 3 E AMERO a Se ee 4 2 1 Available ale EA e EE 4 3 Insertion of the Sample Feedback Temperature Sensor cccccccccccccccccccccccccecccecccecccecccecccescceccees 5 4 Insertion of Sample Holder into Chamber NEE EE EE 5 IVAN eg Be 6 6 Working with 1x3 and 1x2 chamber slides Magnetic lOCKS ccecccccccccccccccccccecccecccecceecceeccess 9 Ts COMMECEIOMCOL CIS Gas SUD DIY nenea ee eee hse esas ee es eee eee 10 B VV OUTING WAIN SUIS O EE 10 9 Connection of the Chamber with EE 11 www oko lab com es H301 UP User Manual November 2014 1 Components and dimensions H301 UP includes the following components Chamber base with embedded temperature sensor Chamber body it owns 12 holes for the insertion of perfusion tubing up to 2 0 mm Objective adapter the dimension and type of objective in use must be specified in the purchase order Holder the type of stage on which the chamber will fit must be specified in the purchase order Heated Lid it features a hinge system allowing to open and close the Lid surro
3. inside the chamber 4 Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by magnets embedded within both on the chamber and on the holder To introduce a sample holder with the proper orientation match the red dot on the holder to the one on the chamber base as illustrated in Figure 4 a ro pe AAT aS Insert the adapter by superimposing the red points Figure 4 Introduction of the Specimen Holder inside the Chamber Base If you want to change the sample holder follow the steps shown in the images of Figure 5 and listed below 1 Open the lock spring as shown in Figure 5 Image 2 2 Open the chamber lid as shown in Figure 5 Image 3 www oko lab com Sec H301 UP User Manual November 2014 Remove the chamber lid by lifting it from the workspace as shown in Figure 5 Image 4 Remove the chamber by lifting it from workspace as shown in Figure 5 Images 5 and 6 5 Lift and replace the sample holder as shown in Figure 5 Image 8 Lock Spring Closed Figure 5 Introduction of the Specimen Holder inside the Chamber Base 5 Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder www oko lab com S H301 UP User Manual November 2014 Figure 6 illustrates the available magnetic locks for 35 and 60 mm dishes Threaded magnetic posts allow adjusting holder s height Magnetic Locks for 35 and 60 mm pe
4. mber slide To lock the slide push simultaneously the buttons indicated with 3 www oko lab com sec H301 UP User Manual November 2014 7 Connection of the Gas Supply A single silicon tubing carries output gas from the Okolab Gas Controller to the H301 UP Silicon tubing connects to a gas input brass opening located on a corner of the H301 UP See Figure 12 Connect by gently pushing silicon tubing onto brass opening Figure 12 Connection with gas supply 8 Working with Perfusion The H301 UP features 12 perfusion holes on the sides for the insertion of perfusion tubing up to 2 0 mm in outer di ameter Small screws plug the perfusion holes when not in use Grub screws M3x6 Remove small screws as necessary before introducing perfusion tubing Figure 13 shows the location of perfusion holes Perfusion dedicated opening 1 Frontal view 2 3D view Figure 13 Perfusion www oko lab com page LO H301 UP User Manual November 2014 9 Connection of the Chamber with stage H301 UP fits on the stage To fix the chamber on the stage follow the steps shown in Figure 14 1 Place the holder on the stage and fix it by the four pan head screws M2 5x6 The place of the pan screws is indi cated by letter A See Figure 14 Image 1 Place the chamber on the stage and fix it by the four pan head screws M2x4 The place pan screw is indicated by letter B See Figure 14 Image 2 e Screw M2x4 Screw M2 5x
5. tri dishes Included in the corresponding sample holder Figure 6 Magnetic locks for 35 and 60 mm dish NOTE Magnetic locks are included with sample holder Spacing rings with variable diameter are also included in according to the needs of the user Figure 7 shows how to position the ring inside the sample holder Ring selection guide diagrams rings for 35 and 60 mm dishes Figure 8 and Figure 9 respectively Figure 7 1 Insertion of the ring 2 insertion of the 35 mm dish 3 insertion of the magnetic lock NOTE Spacing rings are included with sample holder www oko lab com e H301 UP 2 BD Falcon 35x10 Grainer Petri35x10 Willco 35x10 Corning 35x10mm Figure 8 Ring selection scheme for 35 mm dish C Corning 60x15mm www oko lab com User Manual November 2014 Ibidi p Dish35mm low MatTek P35G X 14 X Ibidi p dish 50mm low CO BD Falcon 60x15 CO Willco60x15 Figure 9 Ring selection scheme for 60 mm dish MatTek P50G X 30 X Grainer Petri 60x15 Page H301 UP User Manual November 2014 6 Working with 1x3 and 1x2 chamber slides magnetic locks Magnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder Figure 10 Magnetic lock for 1 x 3 chamber slide Figure 11 Magnetic lock for 1 x 2 cha
6. unding the objective Total chamber weight 170g Objective adapter 28 0 Chamber Body D130 Sample Holder Order Separately Chamber base 87 60 Chamber top view Figure 1 H301 UP Components and Dimensions www oko lab com be H301 UP User Manual November 2014 2 Sample Holders 2 1 Available Sample Holders The following sample holders are available NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the list 1 35mm Petri dish 1 1x3in chamber slide 1 Lab Tek 1in x2in chambered cover glass 1 Lab Tek II 1in x2in chambered cover glass 1 60mm Petri dish UP 1x35 M UP 1x60 M UP 1xGS M For 1 35mm Petri dish holder For 1 60mm Petri dish holder For 1 1 x3 chamber slide holders UP 1xLABTEK M UP 1xLABTEK II M For 1 1 x2 Labtek For 1 1 x3 chamber slide and chambered cover glass holder 2 35mm petri dishes Figure 2 Available sample holders www oko lab com Page H301 UP User Manual November 2014 3 Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H301 UP main body see Figure 3 Frontal and 3D views Temperature Sensor dedicated opening Temperature Sensor dedicated opening 1 Frontal view 2 3D view Figure 3 Insertion of the temperature sensor
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