Scorpion Plate Stacker
Contents
1. PLATE STACKER Scar pon rm TT Bd dg Ie 7 jg 1 1 3 i t 4 zd 2 f A a WM m Version 1 4 Scorpmon PLATE STACKER CONTENTS Introduction Caution Notes Before using the Scorpion Positioning Connecting the Mains Supply Electrical Air Integration with External devices owitching Dual Mode Operation Connecting the Supplies Connecting the Control Pod Controlled Stop Optimisation Transfer Set up Advanced Set up Gripper Set up Hotel Set up Additional Set up Communication Protocol Service amp Maintenance Helpful Hints CE Certification Contact Information Version 1 4 15 16 17 19 20 21 22 29 TBC 31 32 33 34 35 Page 2 Scorpmon PLATE STACKER INTRODUCTION The Scor pon PLATE STAcKER has been specifically created as an automated high throughput plate handling system to integrate with most stand alone laboratory automation equipment The system is designed to be an entry level automation system with the same basic functions as many larger systems with the capability of addition and expansion to handle different types of media SPECIFICATION Size 620mm x 230mm 305mm at front x 900mm LxWxH Capacity 2 Hotels 680mm high capacity 1 full 1 transfer 72 off 384 well PCR Plates i e ABgene TF 0384 54 off 96 well PCR Plates i e ABgene AB 0800 40 off 96 384 Dish Media Plates i e Nunc 1670082. 1 4 18 Scorpmon PLATE STACKER HOTEL SET UP Once you have followed the previous steps carry out the following steps to set up the hotels with the MTP s to be used The majority of MTP s work with the stacks in the default set up position however it is good practice to check this before commencing the first run ilic Place the Hotel stack onto a clear bench 2 Ensure that the adjustment knobs are in the default position with the red dot facing into the hotel T 3 Lay the hotel stack down with the base accessible on the end of the bench Insert a new plate into the hotel stack with the underside of the plate facing towards the bottom Ensure that the plate is prevented from passing through the stack by the retractable grippers Note If the plate passes through check again that the adjustment knobs are facing in If the plate is still allowed to pass through you may be using an unsupported plate type and you should contact the distributor for further advice 6 Remove the plate from the stack and re insert with the top side well opening facing towards the bottom 7 Ensure the top of the plate the section above the skirt is not gripped by the retractable grippers and moves freely 8 If the plate does not move freely rotate the adjustment knobs either clockwise or counter clockwise in equal amounts so that there is clearance on the sides of the plate J Version 1 4 Pag
3. j Figure 1 5 Controlled Stop Pushbutton The Scar has preventative measures inbuilt to remove any danger of entrapment of body parts in the moving arm or possible damage to an external device and therefore the use of the Controlled Stop is seldom required In cases of collision with an external device or entrapment of body parts the moving axis of the Scor pon the Controlled Stop can be pushed to immediately stop the axis from moving however power will remain on the unit with the drives in an energised state In this state if a MTP is being held in the gripper arms to prevent possible spillage of sample the MTP will remain gripped until instruction form the user to release Ensure the Scorpion transfer bed is clear of obstructions The Open Stacker Gripper screen is displayed If a plate is present in the gripper arms press Yes The unit will release the plate and display the Initialise screen Press Proceed The unit will reset the drives At this point check the transfer bed is clear from obstructions as the hotel may have been in the process of releasing a plate To continue the run from that point Press Run The unit will continue to Run in normal operation Version 1 4 Page 14 Scorpmon PLATE STACKER TRANSFER SET UP Before commencing a run the software set Transfer Position needs to be defined Turn the power on to the unit with the Rocker switch on the Side Cont
4. 4 Position the External Device on the left hand side of the Scorgon locating the feet into the holes The Standard Integration Plate will fit the following devices into the specified holes Holes A ALPS300 Holes B ALPS3000 Chameleon Version 1 4 Page 7 Scorpmon PLATE STACKER CONNECTING THE ELECTRICAL SUPPLY Note The system is self regulated to sense voltage differences between 100 and 240 volts AC 1 Remove the external power supply from the packaging and connect the IEC lead specific to your location into the IEC connector on the power supply 2 Connect the lead to the mains power supply and if present switch on 3 Check that the Green indicator LED on the top of the power supply is illuminated Prior to inserting the DC Connector ensure the rocker switch on the Side Control Panel of the Scar ionis in the Off position 4 Insert the DC Connector into the inlet marked 24V DC SUPPLY on the Rear Inlet Panel At this time do NOT switch the rocker Switch to the On T position Figure 8 1 Rear Inlet Panel Electrical Supply Figure 8 2 Side Control Panel Version 1 4 Page 8 Scorpmon PLATE STACKER CONNECTING THE AIR SUPPLY U K Europe and Asia supplied units The unit is supplied with a 6mm push in connector Blue to fit into the 74 BSP inlet U S A supplied units The unit is supplied with a 77 push in connector Orange to fit into the B
5. Transfer Total time inc 7 second Chameleon sealing sequence 20 seconds max Times Therefore 24 minutes for 72 384 well plate cycle or 18 minutes for 54 96 well plate cycle INTEGRATION The Scor can be integrated with the following External Devices If your product is not the list consult your Distributor or KBiosystems for further assistance ALPS3000 e ALPS300 Chameleon KAPS 500 External PC as Slave Device OPTIONS e Left Handed Version e Lidded Plate Hotels e Lid Handling Barcode Reading e Gripper Arm options for different plates Version 1 4 Page 3 Scorpmon PLATE STACKER CAUTION NOTES It is Important that the Scorpion is installed and operated in such a way that all applicable Heath and Safety requirements are met It is the users responsibility to ensure that all relevant Health and Safety Regulations are identified and complied with Failure to do so may result in damage to the equipment and could cause personal injury In particular the user should study the contents of this guide carefully before handling or operating this equipment All users should be fully trained and have a full operational knowledge of the equipment Under No circumstances will the supplier of this equipment be liable for any incidental consequential or special damages of any kind whatsoever including but not limited to lost profits arising from or in anyway connected with the use of this equipment or
6. replies ok lt cr gt Arm moves from home position to external device and takes the plate from the external device the arm then returns to the home position and plate is placed in hotel 2 Replace Plate 4 PC sends R4 lt cr gt Scorpion replies ok lt cr gt Take s the plate from the external device and arm moves up Replace Plate 5 PC sends R5 lt cr gt Scorpion replies ok lt cr gt Arm moves to home position and moves plate under hotel 2 on the transfer belt The user then sends a new G s command which moves the plate up into hotel 2 and removes a new plate from hotel 1 The G s amp R s commands continue until all plates have been sealed and the status bit 5 is set to signal no more plates in hotel 1 Version 1 8 Page 25 Scorpmon PLATE STACKER COMMUNICATIONS PROTOCOL Restack Hotels PC sends S lt cr gt Scorpion replies ok lt cr gt The plate will restack from the Destination Hotel to the Source Hotel in the same order they were loaded until there are no plates remaining Error Message PC sends E lt cr gt Scorpion replies E lt cr gt Where is a decimal value represented in ASCII i e the characters 03 means arm up error Error numbers Arm up error Arm down error Plate transfer back error Arm home error Lift home error Conveyor jam Pause button was pressed ON DD Set transfer position PC sends P1 lt cr gt Where is the transfer position value 0 t
7. this instruction manual WARNING SYMBOLS In accordance with IEC 417 Risk of Electric Shock Moving Equipment Machinery May Move Without Warning Earth Protective Conductor Mains Switch Symbols gt OPPS I ON 0 OFF Version 1 4 Page 4 Scorpmon PLATE STACKER CAUTION NOTES Statement Warning Do not operate this instrument in an atmosphere containing explosive gases Statement Warning Only approved supplied mains cord set must be used with this instrument Statement Warning If it is required to use an extension lead the lead MUST be earthed amp tested independently before use with KBiosystems equipment Statement Voltage The Scor is supplied with an external power supply for direct connection to normal 100V 240V AC supply 50 60Hz with an output of 24V DC 2 5A amp 60W e If supplied safety guards MUST be used at ALL times during the operation of the unit e The unit should only be used in a suitably ventilated area The use of solvents on the unit is not recommended e Certain components move during the correct operation of the equipment and due care should be taken to avoid personal injury e The Electrical Supply disconnected prior to the removal of any safety guards e KBiosystems Limited accepts no responsibility for the misuse of this equipment The Mains Plug supplied with the Scor gon stacker is fitted with the following fuse Supply Fuses Fitted in Plug Fuse
8. ER Standard Integration Plate 2x Hotels 2x Hotel Inserts LCD Control Pod 24V dc Power Supply Mains IEC Lead UK Mains IEC Lead USA Mains IEC Lead EU RS232 Extension Lead 1 RS232 Communications Lead 6mm Push In Air Fitting Blue 74 Push In Air Fitting Orange Tool Kit Instruction Manual Warranty Documentation Note If any components from the above list are missing contact the distributor immediately for replacement Version 1 4 Page 6 Scorpmon PLATE STACKER POSITIONING The Standard Integration Plate accommodates the Scorpion and the most commonly integrated units ALPS300 ALPS3000 Chameleon Integration Plates to suit other products are available and can be purchased through KBiosystems or your distributor When positioning the Scorpion erate stacker ensure there is sufficient space on a sturdy bench for movement and accessories Once the Scorpion is on a bench ensure that no shipping material is visible on in or around the system Place the Integration Plate onto the bench with the smaller plate upwards toward the front of the bench and on the right hand side 2 Lift the Scor gon onto the Integration Plate locating the middle and rear feet of the unit into the 4 holes in the smaller plate 3 Push down on Scar ion to ensure a good secure fit Note the feet should still be in contact with the bench top Check this by looking at the front feet and making sure the unit is sitting level
9. ION WITH EXTERNAL DEVICES When connecting to an external device there are two ways in which the Scorpion can operate with the device as the master or as a slave Below is a description of each method of operation and the type of devices that each method is applicable to Stand Alone Mode The Scorpion will take control of the external device operating basic commands such as SEAL if used with an automated plate sealer This master control allows the Scor to streamline the procedure dependant on the amount of plates in the hotels variable and the cycle parameters and ready status of the external device The unit will operate as a simple set up and walk away integration and cycle until there are no plates left in the hotel LCD Control Pod required Default Mode Compatible Devices ALPS300 ALPS3000 Chameleon ASP50 KAPS 500 Integrated Mode The Scor pon will act as a slave device to a master controller typically a PC connecting and operating multiple slave devices or the controller of a complex robotic platform The Master controller will set when the Scor gon presents a plate to the system and the parameters of operation i e type amp quantity of plates This type of operation usually requires more in depth set up from the user No LCD Control Pod required e Compatible Devices BiOcto Pic Fusion Thermo Multidrop Integration Mode 0 For use with non lidded plates Integration Mode 1 For use with lidded
10. Release pause push button When re powered the stacker LCD module if connected will display scrolling KBiosystems logo Scorpion logo Software version cont eject open reset screen How TO TELL WHAT MODE THE STACKER IS IN Power up with LCD Control Pod attached If mode is STAND ALONE then LCD will show scrolling Kbiosystems logo Scorpion logo Software version cont eject open reset screen If mode is INTEGRATION then LCD will show scrolling Kbiosystems logo Scorpion logo Software version Integrated Mode screen Version 1 4 Page 11 Scorpon PLATE STACKER INTEGRATION WITH EXTERNAL DEVICES CONNECTING THE SUPPLIES T Connect the required Extension Lead to the correct COMM port on the Rear Inlet Panel ofthe Scor pon Tighten the screw in fixings to secure the plug 2 Connect the other end to the COMM port of the External Device Note This is usually at the rear of the unit Contact the manufacturer for further assistance if problems arise Figure 11 1 Rear Inlet Panel RS232 COMM Port Figure 11 2 Rear Inlet Panel AUX COMM Port Version 1 4 Page 12 Scorpmon PLATE STACKER CONNECTING THE CONTROL POD The Control Pod is for use with the Scorpion stacker when integrating with an external device that will act as the slave unit i e the Scorpion will control when the external device operates but with the parameters remaining locally set Note The Control Pod is not used when the Sco
11. SP inlet When fitting an air connector to the Scorpion PLATE STACKER always use 2 spanners one to tighten the connector and one to prevent the bulkhead fitting from turning These are 14mm amp 17mm respectively If the bulkhead fitting is allowed to rotate it may become loose The air supply for the Scorpion should be 80psi 5 5 bar and a maximum of 87psi 6 bar The supply should be able to provide 50 litres minute through a pressure regulator as there is no regulation of the air within the unit Exceeding the maximum pressure may damage the unit The air supplied must be clean dry and oil free Most air regulators include a water filter but not an oil filter this may need to be fitted separately 1 Fit the required air fitting to the bulkhead fitting the Rear Inlet Panel Ensure that this is tight as an air leak will affect the operating of the unit 2 Connect a length of 6mm or 74 polyurethane tubing of no smaller internal bore of 4mm and of no greater length than 2M to the Scorpion 9 Connect the other end to the main supply and if present open the stop valve or similar Note If the mains supply is greater than 87psi 6 bar then fit a pressure regulatorin line This will have to be tested and set prior to connecting the Scor gon to the main supply as pressure exceeding this may cause ecd the unit Yi Figure 9 1 Rear Inlet Panel Air Supply Version 1 4 Page 9 Scorpmon PLATE STACKER INTEGRAT
12. The lift then moves to the bottom position and the plate is taken by the transfer conveyor Set arm safe position PC sends P4 lt cr gt Where 227 is the transfer position value 0 to 255 Default 50 Scorpion replies ok lt cr gt Read arm safe position PC sends P4 lt cr gt Scorpion replies P4 lt cr gt Where is the transfer position value 0 to 255 Version 1 8 Page 27 Scorpmon PLATE STACKER COMMUNICATIONS PROTOCOL Set arm speed PC sends P5 lt cr gt Where 227 is the speed setting value 1 to 100 Default 100 Scorpion replies ok lt cr gt represents percent where 100 is maximum speed and 1 is 1 of maximum speed Read arm speed PC sends P5 lt cr gt Scorpion replies P4 lt cr gt Where 22 is the speed setting value 1 to 100 Check Firmware Version Pc sends V lt cr gt Scorpion replies Scorpion V where is the firmware version number Set Mode Pc sends M cr Where is the mode value 110 Non lidded plates 1 Lidded plates 2 Lidded plates de lidding Scorpion replies ok lt cr gt The Stacker will prime the stacks after mode 1 or mode 2 has been set The Stacker will initialize after mode 0 has been set All the above listed commands can be used The following commands can currently be used in mode 1 and mode 2 I G R S T J1 J2 E P1 P3 P4 P5 M Read Mode Pc sends M lt cr gt Scorpion replies cr Wh
13. To calculate the drop position parameter in millimeters 0 1 resolution Measure from the top of the lid to half way across the skirt of the plate note you may still need to adjust this figure up down Example If the measurement is 15 0 mm the plate drop value is 150 Generally use the above procedure to obtain a rough setting and then adjust figure accordingly when you do initial tests with the actual plates If the plate or lid normally the lid do not eject from the hotel then increase the drop position parameter If more then one plate lid is ejected form the hotel then decrease the drop position parameter PLEASE NOTE YOU MAY DAMAGE THE ODD PLATE LID IN THE PROCESS OF SETTING UP Version 1 4 Page 30 Scorpmon PLATE STACKER HELPFUL HINTS CALCULATING THE DROP POSITION Non Lidded Plates To calculate the drop position parameter in millimeters 0 1 resolution Measure the thickness of the skirt of the plate note you may still need to adjust this figure up down Example If the measurement is 12 5 mm the plate drop value is 125 Generally use the above procedure to obtain a rough setting and then adjust figure accordingly when you do initial tests with the actual plates If the plates do not eject properly from a hotel then increase the drop position parameter If more than one plate is ejected from a hotel then decrease the drop position parameter Lidded Plates To calculate the drop position param
14. e 19 Scorpmon PLATE STACKER HOTEL SET UP The hotels are now set up for the plate type These can now be loaded onto the Scor in the positions shown on the side cover with the full hotel Source at the rear and the empty hotel Destination at the front Ensure that the hotel lock is applied before commencing a run Source Full Destination Empty With the plates loaded into the Source Hotel insert the Plate Weights on to the top plate the Source and into the bottom of the Destination Hotel These prevent the plates from tipping when being inserted back into the hotel and to ensure light plates are ejected from the hotel They will not pass through the hotel locks during normal operation and will therefore not affect the run Version 1 4 Page 20 Scorpmon PLATE STACKER ADDITIONAL SET UP The Transfer Bed on the Scorpion PLATE STACKER has manually adjustable guides to set the position the plate is presented to the griper arms and subsequently to the external device These adjustments are usually required after initially running the unit where the plate transfer position may need to be altered This should be performed when in the OFF state where the gripper arms are in a closed position and the arm can be moved Move the arm to a safe position away from the transfer bed PLATE STOP If the plate is contacting with either front or back of the external device plate shuttle Loosen but do not
15. e stacker GRIPPER SET UP The Gripper Arms on the Scorjaon PLATE STACKER are manually adjustable to set the position the plate is gripped and to prevent over gripping and therefore deforming flexible plates such as vacuum formed polystyrene plates This should be performed when in the OFF state where the gripper arms are in a closed position and the arm can be moved Ensure there is an air supply to the unit HEIGHT SET UP 1 Place a plate on the Scorpiontransfer bed in the transfer position 2 Look across the transfer position to check if the top of the gripper arms are approximately in line with the top of the plate If not then o 3 Remove the 2 fixing screws in the Gripper L arm using the 2 5mm Allen Key supplled and move the arm to one of the positions closer to the required height t 4 Replace the fixing screws Ensure the arms are set at the same height and are level WIDTH ADJUSTMENT Note This will not need to be 6 Adjusted for rigid plates ANNE 1 Turn the Adjuster Knob cl ckwise pu until it reaches it s limit ERN p 22 2 Place a plate on the the X 522 until all four pins in contact with the side of the plate 6 and until the plate starts to deform bed in the transfer position 2429 minim mim Ss A E 3 Turn the Adjuster Knob anti clockwise The I 59 2 x gt A arms will move to grip the plate Continue adjusting 255565555 fl E 1
16. ere is the mode set returns O for non lidded plate returns 1 for lidded plates returns 2 for lidded plates with de lidding Version 1 8 Page 28 Scorpmon PLATE STACKER COMMUNICATIONS PROTOCOL SWITCHING OUTPUTS VIA COMMS Pc sends K 1 lt cr gt where is the output number Scorpion replies OK lt cr gt Example Pc sends K1 1 lt cr gt SWITCHES OUTPUT 1 ON Scorpion replies OK lt cr gt Pc sends K1 0 lt cr gt SWITCHES OUTPUT 1 OFF Scorpion replies OK lt cr gt Output listing Output 1 Conveyor on forward direction Output 4 Hotel 2 gripper open close Output 5 Hotel 2 gripper open close Output 6 Gripper Arm up down Output 7 Conveyor on reverse direction Output 9 Gripper open close Version 1 4 Page 29 Scorpmon PLATE STACKER HELPFUL HINTS CALCULATING THE DROP POSITION Non Lidded Plates To calculate the drop position parameter in millimeters 0 1 resolution Measure the thickness of the skirt of the plate note you may still need to adjust this figure up down Example If the measurement is 12 5 mm the plate drop value is 125 Generally use the above procedure to obtain a rough setting and then adjust figure accordingly when you do initial tests with the actual plates If the plates do not eject properly from a hotel then increase the drop position parameter If more than one plate is ejected from a hotel then decrease the drop position parameter Lidded Plates
17. esented ASCII i e the characters ff means Oxff hex Scorpion replies lt gt Response to any incorrect invalid commands Bit values bit 1 error bit2 busy bit 4 restacking bit5 finished stack run init command needed to reset this bit When checking the communication using for example Hyper terminal the responses to the status message enquiry are hexadecimal values which when converted to binary values allow the status to be interpreted For example when the unit is busy the response is 04 In binary this is 00000100 NOTE cr carriage return NOTE Hotel 1 refers to the plate stacker hotel furthest from the gripping arm NOTE Hotel 2 refers to the plate stacker hotel nearest to the gripping arm Version 1 4 Page 22 Scorpmon PLATE STACKER COMMUNICATIONS PROTOCOL COMMANDS Initialise PC sends l lt cr gt Scorpion replies ok lt cr gt Finish Run PC sends C lt cr gt Scorpion replies ok lt cr gt Pushes a plate under hotel 2 up into hotel 2 does not take plate from hotel 1 Finish Run2 This command can only be used in conjunction with Get Plate 1 lt G1 gt command PC sends C1 lt cr gt Scorpion replies ok lt cr gt Plate is moved under hotel 1 from plate grip area and pushes the plate up into hotel 1 does not take plate from hotel 2 Get Plate PC sends G lt cr gt Scorpion replies ok lt cr gt Take s a plate from hotel 1 and moves to the front grippers and
18. eter in millimeters 0 1 resolution Measure from the top of the lid to half way across the skirt of the plate note you may still need to adjust this figure up down Example If the measurement is 15 0 mm the plate drop value is 150 Generally use the above procedure to obtain a rough setting and then adjust figure accordingly when you do initial tests with the actual plates If the plate or lid normally the lid do not eject from the hotel then increase the drop position parameter If more then one plate lid is ejected form the hotel then decrease the drop position parameter PLEASE NOTE YOU MAY DAMAGE THE ODD PLATE LID IN THE PROCESS OF SETTING UP Version 1 4 Page 31 Scorpmon PLATE STACKER HELPFUL HINTS INTEGRATION EXAMPLE The ok response from the Stacker is only acknowledgement of a valid command i e the command sent to the Stacker was validated and has been accepted It has does not indicate when a task has completed You can use the cr status command to determine if a task has completed The rs232 communication on the Stacker is disabled during a task only when a task is completed and the Stacker is idle will the rs232 communication be restored So during a task you will receive no response from the Stacker At the end of a task you will receive the status byte if you send lt cr gt This method is preferred because if there is an error during a task the Stacker status response wil
19. l reflect that Example Send command G lt cr gt Wait 100ms Was ok cr received gt no then resend command Yes go to loop1 Loop1 Wait 100ms Send cr Was there a response no then go to loop1 Yes Was response 00 lt gt gt yes then task completed with no errors No Was response 02 lt gt gt yes then get error i e send E lt cr gt Else process other status bits Version 1 4 Page 32 Scorpmon PLATE STACKER DECLARATION OF CONFORMITY As detailed under the European Machinery Directive 89 392 EEC amended by 91 368 EEC and under the UK legislation The supply of Machinery Safety Regulations 1992 SI 1992 3073 ii As detailed under The Electromagnetic Compatibility Directive 89 336 EEC amended by 91 263 EEC and 92 31 EEC and the UK legislation the Electromagnetic Compatibility Regulations 1992 As detailed under The European Low Voltage Directive 73 23 EEC amended by 93 68 EEC and the UK legislation The Electrical Equipment Safety Regulations 1994 The Declaration of Conformity is provided for the following equipment Equipment Serial Number Supplier into the EU Scorpion PLATE STACKER To be included KBiosystems Limited Units 5 to 10 Paycocke Close Basildon Essex 5514 3HS Transposed Harmonised Standards Other Standards Version 1 4 BSEN 12100 1 Safety machines concepts General principles for design basic terminol
20. moves arm to the transfer position opens grippers and moves to a safe position Get Plate 1 This is an alternate command to Get Plate lt G gt above PC sends G1 lt cr gt Scorpion replies ok lt cr gt Take s a plate from hotel 1 and moves to the front grippers and moves arm up to allow plate barcode to be read if required Get Plate 2 This command is only used in conjunction with Get Plate 1 lt G1 gt command above PC sends G2 cr Scorpion replies ok lt cr gt Arm moves down and plate is gripped Arm then moves to the transfer position opens grippers and moves to a safe position Version 1 4 Page 23 Scorpmon PLATE STACKER COMMUNICATIONS PROTOCOL Get Plate 3 PC sends G3 lt cr gt Scorpion replies ok lt cr gt Take s a plate from hotel 1 default and moves to the front grippers arm moves down and plate is gripped Arm then moves to the transfer position opens grippers and arm moves back to home position Get Plate 3 Hotel 1 PC sends G3H1 lt cr gt Scorpion replies ok lt cr gt Take s a plate from hotel 1 and moves to the front grippers arm moves down and plate is gripped Arm then moves to the transfer position opens grippers and arm moves back to home position Get Plate 3 Hotel 2 PC sends G3H2 lt cr gt Scorpion replies ok lt cr gt Take s a plate from hotel 2 and moves to the front grippers arm moves down and plate is gripped Arm then moves to the transfer position ope
21. ns grippers and arm moves back to home position Get Plate 4 PC sends G4 lt cr gt Scorpion replies ok lt cr gt Take s a plate from hotel 1 and moves to the front grippers and moves arm to the transfer position The plate is not lowered on to target Get Plate 5 PC sends G5 lt cr gt Scorpion replies ok lt cr gt Arm lowers and the gripper opens placing plate on target arm then moves to a safe position Version 1 8 Page 24 Scorpmon PLATE STACKER COMMUNICATIONS PROTOCOL Replace Plate PC sends R lt cr gt Scorpion replies ok lt cr gt Take s the plate from the external device and moves it to under hotel 2 on the transfer belt The user then sends a new G command which moves the plate up into hotel 2 and removes a new plate from hotel 1 The G amp R commands continue until all plates have been sealed and the status bit 5 is set to signal no more plates in hotel 1 Replace Plate 3 PC sends R3 lt cr gt Scorpion replies ok lt cr gt Arm moves from home position to external device and takes the plate from the external device the arm then returns to the home position and plate is placed in hotel 2 default Replace Plate 3 Hotel 1 PC sends R3H1 lt cr gt Scorpion replies ok lt cr gt Arm moves from home position to external device and takes the plate from the external device the arm then returns to the home position and plate is placed in hotel 1 Replace Plate 3 Hotel2 PC sends R3H2 lt cr gt Scorpion
22. o 255 Scorpion replies ok lt cr gt Read transfer position PC sends P1 lt cr gt Scorpion replies P1 lt cr gt Where is the transfer position value 0 to 255 Test transfer position PC sends T lt cr gt Scorpion replies ok lt cr gt The arm moves to the transfer position for the external device Version 1 4 Page 26 Scorpmon PLATE STACKER COMMUNICATIONS PROTOCOL Jog to Target PC sends J1 lt cr gt Scorpion replies ok lt cr gt Arm is jogged 7 transfer increment to the target This command only works after a G4 command or a T command If the transfer position is already at the maximum 255 then the command will return err lt cr gt Jog to Home PC sends J2 lt cr gt Scorpion replies ok lt cr gt Arm is jogged 7 transfer increment to the home This command only works after a G4 command or a T command If the transfer position is already at the minimum 0 then the command will return err cr Adjust Drop position Pc sends P3 lt cr gt Where is the transfer position value 0 to 250 Default 44 Scorpion replies P3 ok cr The lift moves up into the hotel to lift the plate The hotel gripper is opened at this stage The lift then moves down by the drop position the greater the drop position the longer the hotel gripper is open The lift moves to the top position minus the drop position then fires the hotel gripper in which holds the remaining plates in the hotel
23. ogy amp methodology BSEN 12100 2 Safety of machines basic concepts general principles for design technical principles BSEN 61010 Safety requirements for electrical Equipment for measurement control amp laboratory use BSEN 60204 safety of Machinery electrical equipment of machines section 19 BSEN 50081 2 Electromagnetic Compatibility generic emission standard industrial environment BSEN 61000 6 2 Electromagnetic compatibility EMC Generic standard immunity for industrial environments BS5378 safety signs amp colours Page 33 Scorpmon PLATE STACKER EC DECLARATION OF CONFORMITY Name amp Address of the Responsible Person within the EU Name Alan Shepherd Address KBiosystems Limited Units 5 to 10 Paycocke Close Basildon Essex SS14 3HS Signature of the Responsible Person or Person Empowered to Sign On his behalf Signature Name vc Aca AS SHETH AUS Date fe e es T Version 1 4 Page 34 Scorpmon PLATE STACKER CONTACT INFORMATION UNITED KINGDOM amp EUROPE FLUIDX Limited Chelford Road Nether Alderley CHESHIRE SK10 4SY support fluidx co uk United Kingdom Web www fluidx co uk The information in this user guide including any apparatus Methods Techniques and Concepts described herein are the property of KBiosystems Limited or its Licensees and may not be copied disclosed or used for any purpose not expressly authorised by the owners there of Since KBiosystems Limited con
24. plates no de lidding by Scorpion Integration Mode 2 For use with lidded plates with de lidding by Scorpion Supplied with the are two communication leads marked Extension Lead 1 amp a Standard RS232 Lead These are for use with different devices in Master or Slave operation depending on the communication protocol of the External Device Below is a list of which devices require which lead and to which communication port on the Scorpgon Note As products are introduced this list will be updated however if you re device is not listed below contact your distributor for advice Extension Lead 1 Black ALPS300 RS232 Port ALPS3000 Chameleon KAPS 500 Standard Comms Lead Grey External PC in Integration Mode AUX COMM Port Version 1 4 Page 10 Scorpmon PLATE STACKER SWITCHING DUAL MODE OPERATION SWITCH STAND ALONE TO INTEGRATION MODE 1 Switch Stacker OFF 2 Hold the pause button large red push button on side of main stacker 3 Switch Stacker ON 4 Keep pause button pushed in for approx 3 seconds 5 Release pause push button When re powered the stacker LCD module if connected will display scrolling KBiosystems logo Scorpion logo Software version Integrated Mode screen SWITCH INTEGRATION TO STAND ALONE MODE 1 Switch Stacker OFF 2 Hold the pause button large red push button on side of main stacker 3 Switch Stacker ON 4 Keep pause button pushed in for approx 3 seconds 5
25. r ion is the Slave to an external device such as a PC Controlled Bidcto Pic Refer to the User Manual for the external device for setting and operating the Scorpion When connecting the Control Pod to the Scorpion prate stacker always ensure the unit is switched OFF 1 Place the Control Pod next to the Scorpion in close proximity to the Side Control Panel 2 Connect the 6 Pin plug into the mating socket on the Side Control Panel Note This has a location tab on the inside to ensure correct orientation Secure the Plug to Socket using the screw collar Figure 1 5 Control Pod Connection Note The Control Pod utilises touch screen technology which is susceptible to damage if operated in a moist environment or with wet fingers Ensure when operating the device that fingers are dry and that the unit is regularly cleaned with a dry cloth Version 1 4 Page 13 Scorpmon PLATE STACKER CONTROLLED STOP The Red Push Button on the Prate stacker Side Control Panel acts as a Category 2 controled stop as described and in accordance with BSEN 60204 Safety of Machinery Directive section 9 2 2 Stop Functions This is NOT to be used as an Emergency Stop button in cases of collision or entrapment For cases requiring an immediate stop and disconnection of power supply the Rocker switch located below the pushbutton should be switched to the O position but this is only recommended in the most extreme cases p
26. remove the 2 fixing screws on the Plate Stop using the 2 5mm Allen Key supplied 2 Move the Plate Stop on the slotted holes to suit the required position i e forwards if plate colliding with the front of the shuttle nearest user or backwards for vice versa adjustment 3 Tighten the 2 fixing screws WIDTH ADJUSTMENT Note It is unlikely that this will need to be adjusted for any reason but if required 1 Loosen but do not remove the fixing screws the Plate Guides using the 2 5mm Allen Key supplied 2 Move the Plate Guides on the slotted holes to suit the required position i e inwards if plate is allowed to rotate or outwards if the plate is gripped too tight t is recommended that there is approximately 1mm of movement on both sides of the plate 3 Tighten the fixing screws Version 1 4 Page 21 Scorpmon PLATE STACKER COMMUNICATIONS PROTOCOL SERIAL PORT SETUP Baud rate 9600 Data bits 8 Parity None Stop bits 1 Flow control None All communications via ASCII Connection is via a straight cable not a modem cable PC 9 way female Scorpion 9 way female Pin 2 lt gt Pin 2 Pin 3 lt gt Pin 3 Pin 5 lt gt Pin 5 The unit responds to each message within 100 msec STATUS MESSAGE PC sends lt cr gt Scorpion replies lt status byte gt lt cr gt the status byte is a hexadecimal value repr
27. rol Panel The Control Pod will display the nitialise screen Ensure that the area around the Scorpion is clear of obstructions Press Proceed The unit will initialise moving the arm and lift platforms to the home position The Run Screen will be displayed Press Setup This will proceed to the Setup Menu screen Press Transfer This will proceed to the Transfer Position screen Press Enter This will move the arm to the default position of 100 Jog the arm using the up amp down V arrows and press Enter The screen reverts back to the Setup Menu after each Transfer move Repeat steps Transfer amp Jog until the gripper arms are central about the shuttle and the same distance above the shuttle as they were to the transfer bed of the Scorpgon in the home position approx 2mm Note For reference the setting for ALPS3000 amp Chameleon Is approximately 128 counts and the ALPS300 js 230 counts Version 1 4 Page 15 Scorpmon PLATE STACKER TRANSFER SET UP ADJUSTMENT The Integration Plate as described on page 7 allows for the Scor gonto be adjusted in a lateral direction to meet with the external device The Red section of the plate that locates the Scorpion feet slides on the main plate locking in position with two fixing screws With the External Device plate shuttle bed in the load position and the Scorpion Powered and in the Transfer Position as set up on page 14 1 Loo
28. s Fitted in Platform Mains IEC UK Only 230V 13 Ampere T5AH 250V Only refit the correct type of Fuse The Scor gon stacker should be operated in an environment with a temperature range of 15 C 40 C and a non condensing relative humidity range of 10 80 Version 1 4 Page 5 Scorpmon PLATE STACKER BEFORE USING THE SCORPION When unpacking your Scorpion PLATE STACKER Visually inspect the unit for any damage which may have occurred during shipping Do not use the unit if any damage is present Check that all components are present See packing list below Before removing the unit from the packaging ensure there is a bench with a mains supply and compressed air outlet within 2M To remove the unit first cut both straps from around the case release the clips by rotating them through 90 and fold back the lid Remove any additional components Next remove central packaging from the arm of the unit and once all this packaging is clear remove from the case The unit is supplied in a re useable shipping container that must be retained in case the unit needs to be returned for servicing All shipping case material should be retained in case of future shipment KBiosystems Limited accepts no responsibility for any damage incurred during return shipping unless the unit is packed in the same packaging as it was delivered Note This equipment must be lifted in accordance with EC directive PACKING CONTENTS LISTING Scorpion PLATE STACK
29. sen the two fixing screws on the right hand side of the Integration Plate using the 4mm Allen Key supplied 2 Move the Scor left or right locating the gripper arms centrally over the external device shuttle bed 3 Tighten the two fixing screws For Additional Setting instructions for the transfer position see page 20 Version 1 4 Page 16 Scorpmon PLATE STACKER ADVANCED SET UP Before commencing a run the software set Advanced Settings need to be defined This includes the plate Drop Position and the External Device setup Press Setup This will proceed to the Setup Menu screen Setup A Press Advance This displays the Drop Position Screen This sets the amount of counts the lift platform drops before releasing the hotel lock to remove a plate Using the up A amp down arrows adjust this to the desired height For reference most 20y 384 well plates will be 120 most 96 well plates will be 160 Approx count value is 10 counts for a 1mm V 128 increase in skirt height Dim A See Fig1 12 Once this is set press Enter The screen proceeds to the External Device setup screen Enter If using with an ALPS300 select Yes by toggling through using the m RLFS 386 up A amp down arrows If using another external device leave at the default No V Ho Press Enter The unit then displays the Run screen Figure 1 12 Plate Skirt Dimension Version 1 4 Page 17 SCOr eas
30. stantly strives to improve all of its products we reserve the right to modity equipment and any user instruction manual without prior notice No part of this instruction manual may be reproduced in any form without the prior consent of KBiosystems Limited Copyright KBiosystems Limited 2007 All Rights Reserved Version 1 4 Page 35 Scorpmon PLATE STACKER NOTES Version 1 4 Page 36 Scorpmon PLATE STACKER NOTES Version 1 4 Page 37
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