Treponema pallidum (Spirochete)
Contents
1. Technical Support Contact Biocare s Technical Support at 1 800 542 2002 for questions regarding this product References 1 Hoang MP High WA Molberg KH Secondary syphilis a histologic and immuno histochemical evaluation J Cutan Pathol 2004 Oct 31 9 595 9 Counterstain Counterstain with hematoxylin Rinse with deionized water Apply 2 Center for Disease Control Manual Guide Safety Management NO CDC 22 Tacha s Bluing Solution for 1 minute Rinse with deionized water Atlanta GA April 30 1976 Decontamination of Laboratory Sink Drains to Remove Azide Salts 3 Clinical and Laboratory Standards Institute CLSI Protection of Laboratory Workers from Occupationally Acquired Infections Approved Guideline Fourth Edition CLSI document M29 A4 Wayne PA 2014 Biocare Medical LLC 4040 Pike Lane Concord CA 94520 Page 1 of 1 coal Tel 800 799 9499 www biocare net Fax 925 603 80802. Treponema pallidum Spirochete Concentrated and Prediluted Polyclonal Antibody Control Number 902 135 040215 Catalog Number CP 135 A B C PP 135 AA Description 0 1 0 5 1 0 ml concentrated 6 0 ml prediluted Dilution 1 100 1 200 Ready to use Diluent Da Vinci Green N A Intended Use For Research Use Only Not for use in diagnostic procedures Summary and Explanation Spirochete Treponema pallidum is the causative agent of syphilis In the past localization of the spirochete agent was achieved with silver stains such as Steiner s and or Warthin Starry Treponema pallidum can now be successfully localized with immunohistochemical techniques in formalin fixed paraffin embedded tissue Studies have shown this offers a substantial advantage over silver techniques in both sensitivity and specificity The antibody consists of a rabbit purified IgG fraction and is highly specific for spirochete 1 Principle of Procedure Antigen detection in tissues and cells is a multi step immunohistochemical process The initial step binds the primary antibody to its specific epitope After labeling the antigen with a primary antibody an enzyme labeled polymer is added to bind to the primary antibody The detection of the bound antibody is evidenced by a colorimetric reaction Source Rabbit polyclonal Species Reactivity Human others not tested Clone N A Isotype N A Total Protein Concentration 10 mg ml Call for lot specific Ig co
3. dy to use N A OAR 135 T60 60 tests prediluted Ready to use N A intelliPATH Automated Slide Stainer IPR135 is intended for use on the intelliPATH Automated Slide Stainer Refer to the intelliPATH Automated Slide Stainer manual for specific instructions on its use When using the intelliPATH peroxide block with intelliPATH Peroxidase Blocking Reagent IPB5000 may be performed following heat retrieval Protocol Recommendations ONCORE Automated Slide Staining System OAR135 is intended for use with the ONCORE Automated Slide Staining System Refer to the ONCORE Automated Slide Staining System User Manual for specific instructions on its use Protocol parameters in the ONCORE Automated Slide Stainer Protocol Editor should be programmed as follows Protocol Name T pallidum Rb OR Tpallidum Rb AP Protocol Template Description Rb HRP Template 1 OR Rb AP Template 1 Dewaxing DS Option DS2 Antigen Retrieval AR Option AR2 low pH 103 C Reagent Name Time Temp T pallidum Rb 30 min 25 C Technical Note This antibody has been optimized for use with Biocare s MACH 4 Universal HRP Polymer Detection and ONCORE HRP and AP Detections Other Biocare polymer detection kits may be used however users must validate incubation times and protocols for their specific application Use TBS for washing steps Limitations This product is provided for Research Use Only RUO and is not for use in diagnostic procedure
4. ncentration Epitope Antigen Treponema pallidum Spirochete Cellular Localization Corkscrew bacteria Positive Control Spirochete infected tissues Known Applications Immunohistochemistry formalin fixed paraffin embedded tissues Supplied As Buffer with protein carrier and preservative Storage and Stability Store at 2 C to 8 C Do not use after expiration date printed on vial If reagents are stored under conditions other than those specified in the package insert they must be verified by the user Diluted reagents should be used promptly any remaining reagent should be stored at 2 C to 8 C Staining Protocol Recommendations intelliPA TH and manual use Peroxide Block Block for 5 minutes with Biocare s Peroxidazed 1 Pretreatment Solution recommended Reveal Pretreatment Protocol Heat Retrieval Method Retrieve sections under pressure using Biocare s Decloaking Chamber followed by a wash in distilled water alternatively steam tissue sections for 45 60 minutes Allow solution to cool for 10 minutes then wash in distilled water Protein Block Optional Incubate for 5 10 minutes at RT with Biocare s Background Punisher Primary Antibody Incubate for 30 minutes at RT Probe N A Polymer Incubate for 30 minutes at RT with a secondary conjugated polymer Chromogen Incubate for 5 minutes at RT with Biocare s DAB OR Incubate for 5 7 minutes at RT with Biocare s Warp Red IPR 135 G10 10 ml prediluted Rea
5. s Suitability for specific applications may vary and it is the responsibility of the end user to determine the appropriate application for its use Precautions 1 This antibody contains less than 0 1 sodium azide Concentrations less than 0 1 are not reportable hazardous materials according to U S 29 CFR 1910 1200 OSHA Hazard communication and EC Directive 91 155 EC Sodium azide NaN used as a preservative is toxic if ingested Sodium azide may react with lead and copper plumbing to form highly explosive metal azides Upon disposal flush with large volumes of water to prevent azide build up in plumbing Center for Disease Control 1976 National Institute of Occupational Safety and Health 1976 2 2 Specimens before and after fixation and all materials exposed to them should be handled as if capable of transmitting infection and disposed of with proper precautions Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with reagents and specimens If reagents or specimens come in contact with sensitive areas wash with copious amounts of water 3 3 Microbial contamination of reagents may result in an increase in nonspecific staining 4 Incubation times or temperatures other than those specified may give erroneous results The user must validate any such change 5 Do not use reagent after the expiration date printed on the vial 6 The SDS is available upon request and is located at http biocare net
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