TE Thermo-cycler User Manual
Contents
1. 21 1 UNPACKAGE Before opening of the package be sure that the package is not upside down Open the package carefully Check the items in the package according to the item list in the package Preserve the package materials they will may be needed in the future transportation Check the outlook of the machine and other items if there is any damage due to transportation Please inform the supplier immediately Please fills the warranty and sent it back to manufacturer as soon as possible so we can supply after selling service at any time you want Page of 23 2 INSTALLATION 2 1 Installation filed Please choose a stable and anti humidity platform to install the TE Thermo cycler the hight of the platform should be well suitable for operation The rear panel of machine should be at least 25cm away to the wall to ensure the ventilation The power supply should be at least 10A and be well connected to earth 2 2Environment The machine can only work under the following environment Environment temprerure 5 C 40 C Relative humidity lt 80 Not directly under sun shine Far away from heat source water amp intensive electromagnetic source 2 3 Power on 1 Connect the power line to the machine 2 Turn on the power switch at the rear panel of the machine Hint Before turn on the power make sure that the power source2. 4 4 3 Edit 4 4 4 Save 8 4 5 Resue 9 4 6 Run 10 4 7 Pause 11 4 8 StOp 12 4 9 Unusual power off 12 4 10 Turn off the machine 12 4 11 System setting 12 4 12 Heat Lid 14 5 Examples 15 6 Trouble shooting
3. Cycle and press enter enter the following screen 0 Step PCR Press data key input 3 at current cursor position then press enter enter the next screen Step 1 0C Hold Om Os Page 1g of 23 Press data key input 94 at current cursor position and then use gt key move the cursor to the position before the character s in line 2 Step 1 94C Hold Om Os Use data key input 35 at current cursor position Step 1 94C Hold Om 35s Press enter enter the next screen Step 1 Extended time 0m Os Because this experiment does not need extended time function so you need not input any data in this page so press V key turn to next page you can also press enter key enter the next page Step 2 0C hold Om Os Use data key input 55 at current cursor position and then press gt key move the cursor to the position before the character s in line 2 Step 2 55C hold Om Os Use data key input 45 at current cursor position Step 2 55C hold Om 45s Press enter key enter the next screen Page 17 of 23 Step 2 Extenuated time 0 m 0s Press V or enter skip this page Step 3 0C Hold Om Os Use data key input 72 at current cursor position then press gt moves the cursor to the position before the character s in line 2 Step 3 72 Hold Om Os Use data key input 50 at current cursor posit
4. change a stable desk make the desk or machine balance fasten the screw on the shell Heating speed is slow Power supply voltage is not high enough use a voltage regulator Cooling speed is slow The ventilation window at the sides are blocked Environment temperature is high Setting temperature is low make the ventilation Routine clean cooling the Room temperature cooling the Room temperature Warm keeping is not Well The lid is open Running in low temperature for a long time Page 99 of 23 close the lid set the machine hold in high temperature for a moment and swipe up the water on the plate Action error when press Keys Press a wrong key The machine is not connected with earth press the right key connect the machine with earth well well The screen display The power has ever been it s normal off during program check the power running socket and power line The power line is not connected well The screen flash when Power on Power supply voltage is unstable There is a powerful electromagnetic source nearby Environment temperature or humidity is out of limitation use voltage regulator put the machine away from the influence make the environment temperature and humidity normal After the power is on the Fan don t Rotate the Beeper Beeps But LCD Display nothing LCD
5. Module does not Work or cable is not Connected well Turn off the power Connected with the manufacturer Page 93 of 23
6. has a good earth ground connection Hint The power switch and power socket are at the rear panel of the machine Page 9 of 23 3 panel keyboard induction Keys on the control panel t 4 gt Page up key you may page up the display screen when you edit your program Page down key you may page down the display screen when you edit your program Cursor moving key you may press the key to move the cursor to choose the edit item When running the program you may use this key to change the display page Pause Pause key press this key once will pause the current running program press this key again will resume Stop Enter CE a 0 9 Util the program running Stop key press this key will cause current running program exit Enter key press this key will comfim your current operation Clear key press this key will clear current input data Minus key this key is used to define the input minus data Point key Data key these keys all used for data input Function key this key is used to set the system data diagnose the system and temperature adjusting This key is used only by the qualified maintenance engineer users should not have the knowledge of this key Page 3 of 23 4 OPERATION INTRODUCTION 4 1 Turn on the machine Hint Please keep the lid closing during running Hint Do not touch the surface of the sample plate amp heat lid they are hot After turn
7. on the machine you will hear 2 bip s and the machine will enter self checking procedure The screen will display lt EG 3200 Model gt Ver5 0 2007 After that the screen will display the main menu Thermal Profile RUN EDIT SAVE RASUME Now you can operate the machine by keypad operation Hint If you have to turn off the machine during program running better wait 3 minutes before you turn it on again 4 2 Main menu Let s start from main menu After turning on the machine and self check procedure The main menu will display on the screen or when in other status press stop key will back to main menu Thermal Profile RUN EDIT SAVE RASUNE There are four operations in main menu RUN EDIT SAVE and RESUME The cursor is under current choosing operation RUN and EDIT can only operate on the working memory of the machine save operation will save the program in working memory to saving memory RESUME operation is the reverse operation of SAVE Hint Every time you turn on the machine the machine will resume the program you ran last time automatically 4 3 Edit In maim menu press gt key move the cursor to EDIT and press enter will enter EDIT menu see below Segment 01 Hold Cycle End Now you can define the first segment in your PCR program 1 user program may contain up to 15 segments You Page 4 of 23 may define this segment as Hold or cycle or End by moving the cursor in
8. TE Thermo cycler User Manual Dear Customer We recommend you reading the manual carefully amp take care of the important hits before you using it This is the user manual for TE Thermal cycler Important Notes When working under moisture environment The water may be accumulates around the sample plate so do not put any important documents or stuff near the machine There is not any part that should be touched by the user during normal operation so no one is permitted to open the shell of the machine except he is an engineer that is qualified by manufacturer Before the repairing operation the power supply of the machine should be turned off The power supply should be connected with the Ground carefully do not use the unsafely socket INDEX 1 Unpackage 1 2 Installation 2 3 Front panel keyboard introduction 3 4 Operation introduction 4 4 1 Turn on the machine 4 4 2Main menu
9. d pause during Running you may use pause key If your experiment need add enzyme in the midway this function is needed During the program running press pause key the following screen will appears Pause 0h0m10s 94C 0h19m40s Has paused for 10sec current temperature is 94 C pause start time is 0 hour 19 min 40sec Press pause again will exit the pause mode Page 41 of 23 4 8 Stop User may stop the running program at any time by press stop key and then the screen will display Press lt Stop gt to abort or lt Ce gt to run again Now you may press stop again to ensure the stop operation or press CE key to recover the program running Hint If the machine has running at a low temperature for a very long time there may be water accumulate on the sample platform this will enlarge the time to reach a high temperature Sweeping away the water will make the machine working better 4 9 Unusual power off If the power supply is suddenly off unnormally during the program is running the machine will remember the break point Once the power supply is recover the machine will run the program from the break point automatically Then several will appear on the screen to indicate that the power has once been off TEOT TETR This indicate that the power Cycle 4 3m0s has S been off 4 10 Turn off the machine The right way to turn off the machine is to turn off the power switch under mai
10. erature up down speed setting page if you want the thermal cycle up down speed at a fixed speed just enter them in this page the value range is from 0 3 3 C for temperature up and 0 2 2 C for temperature down Press enter without setting any data in this page will let the thermal cycle at the maxim speed Page g of 23 Up speed 0 0 C s Down speed 0 0 C s Now we enter the total cycle setting page Total Cycle 0 You may set the cycle times in this page the number may be up to 99 Hint When data input in one segment is finished enter key must be Pressed to ensure the data input and you can enter the next segment One program may contain up to 15 Segments After input the total cycle times press enter key will enter the next segment editing the screen will display as following Segment 02 The 2 segment Hold Cycle End Hold cycle end If the program has only one cycle segment most PCR experiments have only one cycle segment you may use gt key move the cursor to End and press enter the screen will display Profile at End The edit operation is end press Press lt Stop gt to exit Stop key to end the operation Press stop will back to the main menu Thermal Profile Main menu RUN EDIT SAVE RASUME 4 3 3 Hint for Edit operations If you press stop key during editing operation may cause several results 1 when you press stop out of segment editing the screen will dis
11. heat transforming result If choose high quality test rube adding oil can be omitted Hint After insert the test tube in the well turn the test tube in half a cycle in the well to ensure better contact Hint Do not add too many drops of oil in the well the oil overflow will cause the machine working unnormal and short the life of the machine RUN In main menu use gt key move the cursor to RUN then press enter the machine will begin to run the program currently in the working memory space of the machine User may monitor the working status such as heating cooling holding time running time of the machine by watching the screen If the running status information has two pages you may use gt key to browse them For example Up to 94C Blk 55C Cycle 4 3m Os Page 1g of 23 This screen indicate that now the machine is heating target temperature is 94 C current temperature is 55 C this is the 4 cycle when get to 94 C the machine will hold this temperature for 3min 0 sec Press will display next page Step 1 Tot Cyc 25 Oh18m20s Segment 1 This screen indicate that this is the 1st step this segment has 25 cycles total the machine has run for 18 min 20sec This is segment 1 If the program running reach it s end normally the screen will display Program completes Press any Key You may press any key to back to the main menu now 4 7 Pause If the program nee
12. input step of the PCR cycle Choose 1 2 or 3 step PCR by pressing concerning numerical keys press enter to confirm the input and enter the 1 step setting interface The first step 0 C hold 0 min Step 1 0C Hold 0m Os In this page you may set the step s temperature point every temperature point has 3 pages The first page displays the temperature point and the holding time see up You may move the cursor among the parameters by pressing gt and set the data by pressing concerning numerical keys After data set press enter to confirm the set and enter the second page Step 1 Extended time 0m 0s If your PCR cycle has a time extending requirement you may enter the extending time in this page the instrument will add the extending time to holding time after each cycle The extending time may be positive or negative If it s anormal PCR just press enter without any data input to enter the third page Step 1 Extended temp 0 0C If your PCR cycle has a temperature extending requirement you may set it here the instrument will add the extending temperature automatically to the holding temperature after each cycle and the extending temperature may be positive or negative If it s anormal PCR just press enter without any data input to enter step 2 setting If you choose 3 step PCR the 2 and 3 step s parameters setting will be the same as the 1 step After all step are set you will enter temp
13. ion Step 3 72 Hold Om 50s press V key or enter key skip extended time page and enter the next page Total cycle 0 Use data key input 36 as total cycle times at current cursor position and then press enter key enter the next page Segment 04 Hold Cycle End Move the cursor to Hold and press enter Page 1g of 23 Hold at Oc Hold for Om Os Use data key input 72 at current cursor position then use gt key Move the cursor to the position before the character m in line 2 Hold at 72c Hold for Om Os Use data key input 3 at current cursor position then use gt key Move the cursor to the position before the character s in line 2 Hold at 72c Hold for 3m Os Use data key input 20 at current cursor position Hold at 72c Hold for 3m20s Press enter enter the next segment Segment 05 Hold Cycle End Move the cursor to the Hold press enter key Hold at 0C Hold forO0m Os Input data 4 at current cursor position use data key then move the cursor to the position before the character Page jg of 23 COEL Hold at 4C Hold for Om Os Use data key input 4 at current cursor position Hold at 4C Hold for 99m0s 99mim means that the machine will hold at 4 C until user press stop key to end the program running Then press enter enter the next segment Segment 6 Hold C
14. ished the screen will display Prg 10 is saved OK Press lt Enter gt to exit If there is a program already in No 10 prg s memory space the screen will display Prg 10 already exist lt 1 gt Overwrite lt 2 gt Abort You may press 1 to overwrite it or press 2 to Abort current program saving 4 5 Resume If you want to load one program from program memory space to working memory space to run it or edit it you may use RESUME function use gt move the cursor to RESUME in main menu press enter the screen will display Resume Method 0 119 0 You may enter the program No from 0 120 you want to Resume for example No 10 program you should press 1 0 and enter then the screen will display Resume need time Waiting After the Resume operation is finished the screen will display Prg 10 resume OK Press lt Enter gt to exit Pageg of 23 Press enter will back to main menu If there is no program in specified program memory space the screen will display No prg in prg 10 Press lt Emter gt to exit Press enter to back to main menu 4 6 Run Hint Please close the lid of sample plate when run the program to ensure the heat preservation of the plate Hint Please choose the appropriate 0 2ml test tube to get the best heat transforming result Hint Before insert the test tubes in the well please add several drops of Oil in the well to get better
15. n menu Thermal Profile RUN EDIT SAVE RESUME If you turn off the machine during the program is running next time you turn on the machine the machine will keep on the program last time you run 4 11System data setting In main mean Press Util Key the screen will display FUMCTION NO 0 Press lt Stop gt to exit Input 1 and press enter will enter user setting page USER manage lt 1 gt M lt 2 gt S lt 3 gt T lt 4 gt TP Page 49 of 23 1 Simulator or plate temperature choosing Is initially set as plate 2 Temperature changing Speed function switch Is initially set off 3 Time extending function switch Is initially set off 4 Temperature extending function switch Is initially set off Simulator plate mode selecting Press 1 in upper page will enter simulator plate mode selecting page WorkMode Simulator lt 1 gt Sim lt 2 gt Plate press 1 or 2 to select work mode between simulator or plate exp after press 1 the screen will show Change to Simu ctl For 3 seconds and then back to WorkMode Simulator lt 1 gt Sim lt 2 gt Plate then press Enter back to setting page Temperature changing speed function on off setting In user manage setting page press 2 enter temperature changing speed function on off setting page Speed Status ON lt 1 gt ON lt 2 gt OFF 1 speed changing function on 2 speed changing function off Page 13 of 23 If
16. of C hepatitis Reaction condition 1 65 C anti record for 15 minus 2 Pre denaturalization 94 C for 60 seconds 3 3step cycle 94 C for 35 seconds 55 C for 45 seconds 72 C for 50 seconds Total cycle 36 times 4 Keep warm 72 C for 200 seconds 5 long time preservation 4 C 99mins After the program editing save it in program No 8 Operation In main menu Move the cursor to EDIT press enter key enter EDIT screen Segment 01 Hold Cycle End In this screen press key Move the cursor to HOLD and press enter key enter the following screen Hold at 0C Hold for 0m Os Move the cursor to the position before the character C input 65 then move the cursor to the position before the character m Hold at 65C Hold for 0m Os Press date key input 15 at current cursor position Page 15 of 23 Hold at 65C Hold 15m Os Then press enter enter the next segment Segment 02 Hold cycle End Move the cursor to Hold and press enter enter the following screen Hold Hold for Press date key input 94 at current cursor position and then press gt Hold at 94C Hold for 0m Os Move the cursor to the position before the character s in line 2 Use date key input 60 at current cursor position Hold at 94C Hold for 0m 60s Press enter key enter the next segment Segment 03 Hold cycle End Move the cursor to
17. play End current prg Press lt Stop gt to exit Page 7 of 23 If you press stop will end the editing and back to main menu Press any other keys will back to edit page 2 when you press stop in the segment editing the screen will display Abort current seg Press lt Stop gt to exit Press stop key will back to segment editing menu the screen will display Segment 02 Hold Cycle End 3 If you has modified some data in the program editing and press stop key the screen will display Press lt 1 gt to END Press lt 2 gt to abort If you press 1 key the data modifications will be valid and back to main menu If you press 2 key the data modifications will be given up and back to main menu Hint t Jin used to browse the program if you want to browse the segment press enter to enter the segment and then use t and to browse the segment 4 4 Save If you want to save the current edited program permanently you should use SAVE function Press gt to move the cursor to the SAVE in main menu then pre enter enter SAVE menu the screen will display Save to Method 0 120 1 You may chose one No from 1 to 120 as program No for example you chose the No 10 then you should press 1 0 and enter the screen will display Page g of 23 Save need time Waiting The will increase to show the saving process When saving is fin
18. set on temperature changing speed setting page will appear in user program editing pages Else will not This function is set off initially Time extending function on off setting In user manage setting page press 3 enter time extending function on off setting page TimeExtend ON lt 1 gt ON lt 2 gt OFF 1 time extending function on 2 time extending function off If set on time extending function setting page will appear in user program editing pages Else will not This function is set off initially Temperature extending function on off setting In user manage setting page press 4 enter temperature extending function on off setting page TempExtend ON lt 1 gt ON lt 2 gt OFF 1 temperature extending function on 2 temperature extending function off If set on temperature extending function setting page will appear in user program editing pages Else will not This function is set off initially 4 12 Heat lid TE Thermo cycler has a heat lid that can be turned on or off by user The heat lid is set on initially if your PCR experiment need no heat lid you may turn it off In main menu press Util key enter function page FUNCTION No 0 Press lt stop gt to exit Input 3 press enter to enter heat lid control page Warm cap status OFF lt 1 gt ON lt 2 gt OFF Page 44 of 23 1 Turn on the heat lid 2 Turn off the heat lid 5 Operation example Exp 1 Diagnoses
19. the menu 4 3 1 Hold Press gt key move the cursor to Hold and press enter key to confirm your choice Then the screen will display as below Hold at OC Hold the temperature at 0 C Hold for Om Os Hold time is 0 min 0 sec Set holding parameters The data you must enter is holding temperature and holding time min amp sec By pressing data keys you may enter these data If the data you enter is out of the limitation it will be cleared automatically Press CE will clear error data input After setting a parameter you may moving the cursor to the next data by pressing gt Hint If you want to modify the data input you may press gt to move the cursor to target data and then input your new data Hint Temperature limitation 5 C 100 C Time minute limitation Omin 98min Time second limitation 0sec 59sec Hint If you want to input a minus data first input the data and then press to add the in front of the data Hint If you enter a 99 in holding time min position It means that Hold time will not end until you press stop key to stop the program running Page 5 of 23 4 3 2 Cycle Chose Edit in main menu the following menu will display on The screen Segment 01 Hold Cycle End Move the curser to Cycle and press enter to define current segment as cycle segment now the screen will display 2 Step PCR In this menu you will
20. ycle End Move the cursor to END and press Enter key Profile at End Press lt stop gt to exit Press stop key back to main menu Thermal Profile RUN EDIT SAVE RESUME USE gt key move the cursor to SAVE and press enter to SAVE the current program Save to prg 1 120 1 Press data key input 8 and then press enter to save the current program as program No 8 Page 99 of 23 Save need time Waiting After saving is finished the screen will display Prg 8 is saved ok Press lt Enter gt to exit Press enter key back to main menu 6 Trouble shooting The following table will help you to find out the trouble s causes of your machine and give out the solution of these problems If you still can not find out the cause in this table please contact us Page 91 of 23 Problems Cause Solution The machine has no reaction when you turn the power switch on Power cut Power line is not Connected well Fuse is broken turn off the switch and wait for power supply resuming check power line change the fuse Water accumulate Under the machine Environment humidity is high The machine has run at low temperature for a long time try to low the humidity of the room wipe up the water The noise is loud when running The desk is unstable The desk is unbalance The screws on the shell is unscrewed
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