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IKAPURE 12 system 簡易操作手冊 - T

1. system will lead to instrument damages www tprobio com Page 12 Ver JZ89 SO001 V1 0 2 For correct and safe use of T Pro Auto 12t system install it in a location that is close to electrical outlet and has enough space for installation and operation Important Keep the shipping box They will be needed again when transporting the T Pro Auto 12t system 3 Connect Barcode Reader with the Instrument fF www tprobio com Page 13 Ver JZ89 SO001 V1 0 1 5 Overall View E Front view with Sliding Door closed LCD Screen Control Panel Sliding Door 3 s gt Moving Handler TT Proji ana E Front view with Sliding Door opened UV Lamp Operational Area www tprobio com Page 14 Ver JZ89 S001 V1 0 E Back View Power Switch Power Inlet 1 x Barcode Reader Port amp 1 x USB Port 2 Getting Started Biohazard Always wear appropriate gloves a mask and safety goggles during any biohazardous operations in extraction process Even when touching the device after any operation with a biohazard risk wear appropriate gloves and a mask since the device may be contaminated Important Before starting extraction put on appropriate gloves a mask and safety goggles if required by the operation In the operation from preparing samples to extraction completion be careful not to contaminate the samples with sweat saliva etc www tprobio com Page 15 Ver JZ89 SO001 V1 0 2 1 Turning on th
2. 15 Push Enter to confirm Instrument will start to run the protocol program automatically until whole processes are completed Note www tprobio com Page 21 Ver JZ89 SO001 V1 0 ttakes from 30 to 45 minutes to complete the extraction according to reagent types Find the detailed time consumption by checking the handbook of your reagent kits 2 4 After Extraction Operation 1 Take out the Elute Tubes The extraction yields in them Note You can apply quality checking or do downstream study or storage them as what you expect 2 Remove the Cartridges and plastic disposables from the instrument and then dispose of them www tprobio com Page 22 Ver JZ89 SO001 V1 0 3 Cleaning amp Maintaining the T Pro Auto 12t system Two types of maintenance are performed on the the T Pro Auto 12t system Instrument as listed in the table For details on each type of maintenance see below Maintenance Type Performed by Schedule Routine Cleaning of Sample Tray After each use Cleaning of instrument Bi weekly body outside Cleaning of instrument Bi weekly body inside Service Engineer E Clean the Sample Tray with mild detergent and rinse with deionized water Allow the parts to dry before use Clean and disinfect the platform surface by wiping with deionized water followed by 70 ethanol E Clean the instrument body by removing dust gently with a dry soft cloth If the outside of the T Pro Auto 12t system i
3. T Pro Auto 12t System Automated Nucleic Acid Purification System Instruction Manual JZ89 S001 www tprobio com Page 1 Ver JZ89 SO001 V1 0 TABLE OF CONTENTS Page INTRODUC TION siiin a anccuteataacecsteianeneats 3 SAFETY INFORMATION icaisnanoa 4 10 INSTALLATION iirinn 8 1 1 Composition of the T Pro Auto 12t SYSteM cceeeeecseeeeeeeeeeeees 8 1 2 Contents of Reagent Kits ccc ccccceecccceeeseeeeeeseeeseeeeeseeseeeeseeees 9 1 3 Operating Environment Condition cccccsseeeeeeeeseeeeeeeeeeeenens 10 1 4 Unpack the T Pro Auto 12t SYSIOM cccccccsseeeeeeeeeeeeeeeeseeenens 12 De OV CAA OW anera EEEE E EE 14 2 0 GETTING STAR TED siosisieniissvasnesescecavaricctaieevesiesiortacecceustivanduecieaxdecneass 15 2 1 TUNNO OM ANC DOW GN coepere ndan EEEE EEEN 16 22 We Le Dal aION ep a E 16 AO EXTA CUOI ooro n E N 17 2 4 After Extraction Operation ccccccccsssececeseeeeeseseeeseeeeeseeneeeeseeees 22 3 0 Cleaning amp Maintaining the T Pro Auto 12t Instrument 23 4 0 TROUDIESMOOUIN G iivesssnncesacs ac sanescnsasactonssoceassossnsenctesaocsssesseseeessanccesanncccses 24 B20 DCCI IC AtlONN S sorunca a 26 APPENDIX Geena espn mene ereeer errr cee ee eee ree oe cer rnereee reece creer reece 27 www tprobio com Page 2 Ver JZ89 SO001 V1 0 INTRODUCTION Thank you for purchase of the T Pro Auto 12t system which is a fully automated standalone robot that can purify nucleic
4. Tray tightly You will hear a click if the cartridge is placed correctly on the tray You can load 1 12 cartridges on the tray depending on the number of samples that you wish to process 5 Place Reaction Chamber s 6 Place Tip Holder s www tprobio com Page 18 Ver JZ89 SO01 V1 0 lt i 3 f x _ a EEN A ERT oe NS 1b ee CEFER E NAAA 9 Place Sample Tube and Elute Tube to Sample Rack on the bench www tprobio com Page 19 Ver JZ89 S001 V1 0 10 Load the sample s to Sample Tube Note Pretreatments are essential for some sample types before loading to Sample Tube Please refer to the handbook of reagent kits for details Make sure the caps of Elute Tube are open as the figure shown above www tprobio com Page 20 Ver JZ89 SO001 V1 0 11 Place Sample Rack on the instrument platform iS Sr Note Use two hands to handle the Sample Tray Make sure the Sample Tray be placed correctly in the instrument 12 Close the Sliding Door 13 Scan the protocol barcodes to select purification protocol sample volume and elute volume Note There is one protocol barcode paper enclosed in the reagent kit box Protocol s name sample volume and elution volume will be shown on LCD screen after protocol barcode is scanned 14 Follow the instructions displayed on LCD screen to double check the operating steps being completed before program running
5. a the quality of DNA isolated depend on the starting material During shipping some magnetic bead solution may adhere Insufficient amount of to the sealing foil of the cartridge To collect any bead magnetic beads added solution from the foil tap the cartridge to deposit the bead solution at the bottom of the well Ensure that the lysate does not contain any particulate Clogged Tips resulting material that can clog the tip sprout If needed centrifuge in DNA loss the sample prior to the MagPurix purification Store cartridge containing the beads at room temperature Do not freeze the cartridge as the beads may be irreparably Magnetic beads stored damaged or handled improperly Make sure that the beads are in solution at all times and do not dry Dried beads are non functional Remove any magnetic beads using a magnetic separator or Magnetic beads present centrifuge the sample in a microcentrifuge for 1 minute at in the eluate l maximum speed DNA contaminated with Minimize the amount of blood or blood stained sample used heme lt 20 ul blood spot for forensics sample To prevent bubble formation during mixing make sure the Bubbles formed during Ea sample volume is at least the recommended volume listed mixing steps l l l in the manual supplied with Reagent Kits Purified DNA Aliquot purified DNA and store at 4 C short term or 20 C repeatedly frozen and long term Avoid repeated freezing
6. acids within 30 45 minutes With advanced magnetic bead separation technology it enables you to have high quality extraction results Moreover the most user friendly interface makes users free from troublesome parameter settings and maintenance This guide contains important information regarding the safe use of the T Pro Auto 12t system Please read this manual carefully before you start to run the system at the first time especially for Safety Information lf there is any question about how to install or operate it please contact our certified distributors agents or email to our technical support center sales tprobio com T Pro FastEZ Purix Magnetic Bead Purification Process www tprobio com Page 3 Ver JZ89 SO001 V1 0 SAFFETY INFORMATION The meanings of safety precaution marks are as follows N WARNING WARNING indicates a dangerous condition that may lead to death or serious injury N BIOHAZARD BIOHAZARD indicates exposure to a biological agent or condition that may be infectious or harmful N CAUTION CAUTION indicates the possibility of danger that may lead to minor or moderate personal injury CAUTION may also indicate an accident of property damage Important Important shows the important notes for usage as well as prohibited actions Note Note indicates the notes procedures that should be obeyed and supplementary information for use For your safety and that of others foll
7. and thawing thawed DNA contaminated with Maintain a sterile environment while working i e wear DNases gloves and use DNase free reagents Page 25 Ver JZ89 S001 V1 0 5 Specifications The T Pro Auto 12t system moa onmo O O 60 C to 70 C assuming the room temperature of 25 C Weigh T CE LV directive 73 23 EEC EMC directive 89 336 EEC EMC EN61326 1 Safety EN60101 1 www tprobio com Page 26 Ver JZ89 SO01 V1 0 APPENDIX E Replacement Optional Parts Parts No A01001 A01002 Item Barcode Reader Filttered Tip Sample Tube Elute Tube E Product Guarantees A02001 A02002 A02003 The guaranty period shall be one year from the delivery of the product Any part which fails to function properly under normal use provided that all warnings and cautions in this manual are observed during the guarantee period will be repaired by the Ji Feng Biotechnology CO LTD free of charge If the problem is due to the causes listed below some charge may be applied for the repair even within the guarantee period E Any failures due to improper use or that are affected by other devices except for those approved by the Ji Feng Biotechnology CO LTD E Any malfunctions or damages during transportation or due to dropping of the product by a user www tprobio com Page 27 Ver JZ89 SO001 V1 0 E About Services Before you ask for device repairs refer to 4 Troubleshooting to check t
8. aste to perform proper incineration fusion sterilization and or disinfection E When you use a third party to dispose of it outsource this work an operator licensed to handle medical waste subject to special control and give them the manifest of the medical waste at the same time www tprobio com Page 7 Ver JZ89 SO001 V1 0 1 Installation 1 1 Composition of the T Pro Auto 12t system Check that the following are included in the package Contact your local representative if you notice any missing part s Q ty A T Pro Auto 12t system Instrument 1 B Power Cord 1 C Sample Tube Rack 1 D Barcode Reader 1 E User s Manual 1 www tprobio com Page 8 Ver JZ89 SO001 V1 0 Please make all the components are free from damages as soon as you get the system If any damage found please contact your local representative for the instant support Note Ji Feng s global warranty does not cover the damages from the transportation or improper operation 1 2 Contents of Reagent Kits Reagent Cartridge s Reaction Chamber s Tip Holder Pestle essential for some Kit types Filtered tip Sample tube Elute tube Omm OO WO gt www tprobio com Page 9 Ver JZ89 SO01 V1 0 Note Reagent kits are separated purchase Please contact your local agents or representatives to get further info The contents of reagent kits will be various Refer to the handbook of kits enclosed in reagent box for details 1 3 Op
9. drop objects on the T Pro Auto 12t system Also refrain from bumping or knocking it as doing so may cause a failure or malfunction of the T Pro Auto 12t system www tprobio com Page 5 Ver JZ89 SO001 V1 0 If any liquid materials are left inside the device wipe it up a soft paper tissue etc Otherwise the T Pro Auto 12t system may be damaged Repairs to the T Pro Auto 12t system should only be performed by such agencies as are specifically authorized by the Ji Feng Biotechnology CO LTD Only original the Ji Feng Biotechnology CO LTD replacement parts should be used lf the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired About Reagent Kits N CAUTION Note When handling any of the kits refer to the respective handbook Reagents in each kit should be handled by observing the safety information and precautions regarding the kit Extraction should be performed in an appropriate laboratory or workplace The kits are not supplied with the T Pro Auto 12t system Select the desired kit s and order it them separately www tprobio com Page 6 Ver JZ89 SO001 V1 0 About Samples N BIOHAZARD E Always wear appropriate gloves a mask and safety goggles etc when handling any infectious samples About Infectious Wastes E When handling or disposing of infectious materials follow the laboratory guideline or the law regarding infectious w
10. e failure fire or electric shock When relocating the T Pro Auto 12t system disconnect the plug from the outlet first If the power cable is damaged this may cause a device failure fire injury or electric shock AN Caution Do not use the T Pro Auto 12t system in an unstable place such as a slanted surface or a place subject to vibrations It may cause injury or device failure Do not use the T Pro Auto 12t system in direct sunlight or close to a heating device It may shorten the life of the T Pro Auto 12t system or cause a trouble Operating Conditions Items Conditions Temperature C During operation 15 3000 2 www tprobio com Page 11 Ver JZ89 SO01 V1 0 Max wet bulb During operation 29 non condensing temperature C During down time 29 non condensing Temperature gradient C hr 12 or less non condensing Humidity gradient RH day 30 or less non condensing Altitude operating m 1600 or less 1 4 Unpack the T Pro Auto 12t system 1 Open the packing box and take out the instrument and related accessories Important The T Pro Auto 12t system has a weight of more than 45 kgs It should be lift and moved by two persons Hold the moving handler of the instrument from two sides to move it out from the box E Do not hold the plastic outer covering E Do not hold the front panel E Do not hold the door A Caution Improper handling of the movement of the T Pro Auto 12t
11. e power 1 Make sure that the power cable are connected securely to the the T Pro Auto 12t system 2 Connect the plug of the power cable to the outlet 2 2 Preparation The following preparations are required for extraction operation Ka www tprobio com Items to be prepared Gloves Mask Safety Goggles Reagent Cartridge Reaction Chamber Tip Holder Pestle Option Filtered tip Sample tube Elute tube For the preparation of samples refer to the handbook of each kit Provided with the Reagent Kits Page 16 Ver JZ89 SO01 V1 0 2 3 Extraction 1 Turn the power switch on the system will process self testing and then go to steady mode Note The system will block main functions before the completion of self testing process 2 Open the sliding door and remove the sample rack from the instrument 3 Take out Reaction Cartridges and all plastics disposables Reaction Chamber Tip Holder Filtered Tip Pestle Sample Tube Elute Tube from the Reagent Kit box You need one Reaction Cartridge and one set of plastic plastic disposables per sample www tprobio com Page 17 Ver JZ89 SO001 V1 0 4 Place reagent cartridges Important Cartridge should be placed in a flat and stable table when setting up the Reagent Cartridge Set Cartridges in the order of the number from left to right Make sure that Cartridges are inserted in to the Cartridge
12. erating Environment Condition Use the T Pro Auto 12t system in a location that meets the following conditions m The space for the allocation of the T Pro Auto 12t system system must be remained 5 10 cm from instrument to side walls E A location where power can be provided Be sure to use the attached AC adapter E A location where the temperature is 15 to 30 C and humidity is 30 to 80 RH non condensing A location that is flat and stable with no vibration A location away from direct sunlight Block the sunlight by closing curtains or blinds as necessary A location which is well ventilated and not dusty A location where the temperature does not go up and down www tprobio com Page 10 Ver JZ89 SO001 V1 0 suddenly Warming a cold room suddenly or moving the T Pro Auto 12t system from a room with low temperature to a warm room may cause condensation inside the device resulting in abnormal extraction E A location where the temperature and humidity are kept within the specified range far from water taps water heaters humidifiers air conditioners and heaters m A location far from objects which generate strong magnetic fields motors transformers TV audio speakers magnets etc Bringing the T Pro Auto 12t system close to any type of magnetic field may cause a malfunction A Warning Do not use the T Pro Auto 12t system to a location where it is wet or can be splashed with water It may cause a devic
13. he problems If the problems cannot be solved contact your local representative or the relative customer support E Notes for Transporting Shipping the T Pro Auto 12t system When transporting shipping the T Pro Auto 12t system to a new location perform the following tasks Warning Before carrying the T Pro Auto 12t system disconnect the plug from the outlet If the power cable is damaged this may cause a device failure fire injury or electric shock When transporting shipping the T Pro Auto 12t system be sure to perform the following tasks Remove all attached parts Sample Rack Barcode Reader from the T Pro Auto 12t system After completing the above tasks pack the T Pro Auto 12t system in its original shipping box or some other equivalent box www tprobio com Page 28 Ver JZ89 SO001 V1 0
14. ing sample protocol To ensure proper mixing of reagents in the or sample volume is tip and prevent bubble formation during mixing make lower than the sure the sample volume is at least the recommended recommended volume volume listed in the handbook supplied with the MagPurix Reagent Kits Presence of buffer in Motor movements may Perform preventive maintenance annually to ensure the Cartridge Tray not be smooth proper motor movements incorrect placement of plasticware or leakage from tips Leakage from Filtered Tips or uneven liquid Air leakage on the Swap the air leaked Tip with new one handling between Filtered Tip Filtered Tips Blockage of tips Too much starting Decrease the amount of starting material Use the www tprobio com Page 24 Ver JZ89 S001 V1 0 material causing recommended amount of starting material as listed in clumps or aggregates the Reagent Kit manual Handbook DNA Quality Problems Low DNA yield No DNA recovered Eluate containing DNA is discolored DNA is sheared or degraded www tprobio com Decrease the amount of starting material used Be sure to add Proteinase K during lysis if included in the Incomplete lysis protocol Make sure that the sample is completely immersed in the Lysis Buffer Be sure to process sample immediately after collection or Poor quality of starting ai store the sample at appropriate temperature The yield and materi
15. ow the guidelines provided in the following pages concerning the use of the T Pro Auto 12t system www tprobio com Page 4 Ver JZ89 S001 V1 0 About Instrument N WARNING Ignoring the following notations may lead to fire or electric shock In countries other than Taiwan US and Canada use a power cable that meets your country s standard or contact your local distributor Use the AC adapter provided Do not use the T Pro Auto 12t system with voltage other than the voltage specified on the device Do not overload the power outlet with too many devices Do not use the T Pro Auto 12t system with a damaged power plug or a loose socket lf there is dust on the prongs of the power plug or on the plug socket remove it with a dry cloth When you disconnect the plug from the outlet be sure to hold the power plug itself Do not pull the power cable For maintenance disconnect the power plug from the outlet Do not touch the power plug when you hear the crash of thunder Do not pour any liquid on the T Pro Auto 12t system Do not place any objects containing liquid on the T Pro Auto 12t system Doing so may cause a device failure fire or electric shock In the event the device overheats starts to smoke or smells strange immediately unplug the power cable Z CAUTION Never attempt to remodel the T Pro Auto 12t system without the manufacturer s permission Doing so may cause fire or electric shock Do not place or
16. s heavily soiled or if any samples that may cause infection are adhered to the outside of the the T Pro Auto 12t system wipe with a soft paper tissue soaked with 0 5 sodium hypochlorite solution or ethanol www tprobio com Page 23 Ver JZ89 SO001 V1 0 4 Troubleshooting Problem Cause Solution O OSSO Instrument Problems No power the LCD Screen remains blank AC power cord is not Check AC power cord connections at both ends or when the power is connected Use the correct cords turned on LCD Screen turns on Forget to remove the when the power is on packing stuff from Turn off the instrument and remove the packing stuff instrument program does notrun Technical problem Contact your local representative or agent Protocol stops after an Cartridge s but the self testing initial start Plasticwares Reaction Turn off the power and then turn it on again to stop the Chamber Tip Holder program The system will move back to initial state Filtered Tip Pestle Re load them according to the instructions shown in Sample Tube Elute this manual Tube incorrectly Note you could not resume the protocol after stop loaded on the the and you may lose your samples MagPurix 12 system Problem with motion Turn off the power and remove all samples and sensors plasticwares Contact your local representatives Bubbles formed during Be sure to add the sample to tubes prior to starting the extraction Missed add

IKAPURE 12 system 簡易操作手冊 - T

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