USER MANUAL - Loligo Systems
Contents
1. The following diagram shows how to connect the instruments and devices used for running a 3 5 Connect preference oxygen experiment YDAC eSS2A qOld iain jean aqoid usBbAxo Y23q AezAjeue Puauied JAAN u 6 XO N 291989 gSN 9jqeo ezeq jqe 1 zdepy Y NI 4AezAjeue YONI I SS A qOld ess J a b JY LA qoud piouajos usbAxo 5 7 oe usBbAxo Z AY AeA piouajos A r a JY VAIeLA piouajos 4 4 P T JH SAILA pioua jos 4 ro awa Page 15 of 34 3 6 uEye USB camera 1 Connect the uEye USB camera to the PC After a few seconds the software installation will start automatically 2 Click on Install the software automatically Recommended and then on Next 3 Click on continue anyways 4 Click on Finish 5 Open the uEye camera manager by clicking Start gt All Programs gt IDSDuEYE Screen 18 Gee Avail Type Cam ID Dew ID Model Seria UI 154x M 4002708319 Control center Expert mode Additional Functions Automatic ETH configuration Status of device Mr 1 The camera is configured correctly and can be opened 6 If installation was successful the uEYE camera is now shown here 7 Mount the lens on the uEYE camera 8 The uEYE camera is now ready for use with ShuttleSoft Page 16 of 34 3 7 DAQ S instrument 1 Connect the DAQ S instrument to the PC After a few seconds the software installation will start au2. HY ShuttleSoft Destination Directory Select the primary installation directory All software will be installed in the following location s To install software into a different location s click the Browse button and select another directory Directory for Shuttles oft CA Programmer S huttleS oft Directory for National Instruments products D Labyiew Runtime engine nT 4 If you accept the License Agreement please select I accept the License Agreement s and then press Next Screen 4 W ShuttleSoft License Agreement Tou must accept the licenses displayed below to proceed NATIONAL INSTRUMENTS SOFTWARE LICENSE AGREEMENT INSTALLATION NOTICE THIS IS A CONTRACT BEFORE YOU DOWNLOAD THE SOFTWARE ANCOR COMPLETE THE INSTALLATION PROCESS CAREFULLY READ THIS AGREEMENT BY DOWNLOADING THE SOFTWARE ANDYOR CLICKING THE APPLICABLE BUTTON TO COMPLETE THE INSTALLATION PROCESS YOU CONSENT TO THE TERMS OF THIS AGREEMENT AND YOU AGREE TO BE BOUND BY THIS AGREEMENT IF YOU DO NOT WISH TO BECOME A PARTY TO THIS AGREEMENT AND BE BOUND BY ALL OF ITS TERMS AND CONDITIONS CLICK THE APPROPRIATE BUTTON TO CANCEL THE INSTALLATION PROCESS Do NOT INSTALL OR USE THE SOFTWARE AND RETURN THE SOFTWARE WITHIN THIRTY a0 DAYS OF RECEIPT OF THE SOFTVYARE INCLUDING ALL ACCOMPANYING WRITTEN MATERIALS ALONG WITH THEIR CONTAINERS TO THE PLACE YOU OBTAINED THEM ALL RETURNS SHALL BE SUBJECT TO NI S THEN CURRENT RETURN POLICY De
3. o E Ea p e Nf S xi gt af T E ra al 2 3 Zi g 1 amp W 7 5 76 x gt Q H z p o 5 o k e o Le d D I 5 5 p oo l a mi s uj gt al S E a mi ie a fa sais Lu xi A a E m Eja a Page 21 of 34 4 3 Temperature The diagram below shows how to connect the buffer tanks with the cooling heating baths Tubing Cooling bath Pump RE 2 Pump RE 4 Buffer tank INCR Buffer tank DECR Pump RE 1 Pump RE 3 Heating bath Page 22 of 34 4 4 Oxygen The diagram below shows how to connect the buffer tanks with O2 N gt gas bottles The air stones need to be placed into down at the bottom of the buffer tanks The remaining fittings on each buffer tank are connected together ied Tu bing Solenoid valve RE 2 Solenoid valve RE 4 Air stone Air stone Buffer tank INCR e Buffer tank DECR Air stone Air stone Solenoid valve RE 1 Solenoid valve RE 3 Page 23 of 34 5 Using ShuttleSoft 5 1 Start up Start ShuttleSoft by clicking Start gt All Programs gt ShuttleSoft gt ShuttleSoft If the USB hardkey dongle is not connected to the PC an error dialog box pops up see Screen 20 Screen 20 Danio Track exe Start Error X Copy protection error Mo license Found 2689 110000 Fors g igen Annuller Connect the USB hardkey dongle to your PC and wait for the device to be recognized
4. then click on Retry Then ShuttleSoft will ask the user of what kind the input is Screen 21 Type of variable Please choose variable bo control F Temperature Salinity oOWTW Cond 3310 Others If choosing preferred Salinity experiment and WTW Cond 3310 the following screen will apear where user is asked to choose the right COM ports for the two WTW Cond 3310 instruments Page 24 of 34 3 Instrument connections WT Cond 3310 1 Acomo Wait a aiting to connect WT Cond 3310 2 ie ha waiting bo connect After choosing COM port press Connect and ShuttleSoft will try to establish communication with the two WTW 3310 Cond instruments If connection was successful the OK button gets available and the user can continue the initialization by pressing OK Now Screen 22 will now appear Screen 22 s ShuttleSoft 2 2 File Settings Calibration Log Help Oxygen Yoair sat vs time 100 80 60 40 eu 0 11 32 03 tleo Interval min DATA INCR O2 DECR O2 0 D Object zone Object O2 OFF 0 INCR visits DECR visits l 0 0 Distance pix Velocity pix s lo 0 SETTINGS Delta air sat Hysteresis 15 0 J0 10 VIEW Mask Binary Trace Length s j 10 RE1 RE2 RES RE 4 Page 25 of 34 ShuttleSoft will connect automatically to the first uEye camera found If no uEye camera is found ShuttleSoft will detect all USB cameras connected to the PC and ask the us
5. IDS vEyve camera Family Please check first whether the hardware of your camera is compatible with this driver version Wihakshew txt Show File with the latest information regarding this driver release Check hardware Check the compatibility of your camera hardware with this driver version oCheckUSBbus Show all USB controllers Install driver Start the setup program For the driver installation noise m g 20 Select Install driver 21 Choose language and then click Next 22 When Screen 15 appears click Next Screen 15 IDS uEye Welcome to the InstallShield Wizard for uE pye Please read the file Whats ew tat before working with Eye You can choose to read the file at the end of the Installation process Also visit Http wii uepre com for the latest drivers Page 11 of 34 23 When Screen 16 appears select Complete and then click Next Screen 16 IDS uEye Setup Type Select the setup type that best suits pour needs Click the type of setup you prefer Se os Eciton 2 USB and Gigabit Ethernet Installation of all components 3 USB 4 Custom 24 Choose a destination and then click Next 25 Select a program folder and then click Next 26 Click Install to start the installation 27 Select the additional features you want then click Next Screen 17 Additional features Select or clear additional features Install a desktop shortcut to WEye Demo and to uEye Camera Ma
6. or metres Change the length and or position of the green line to match any known distance in the image e g a ruler The length of the green line will show Then choose the desired unit of measure and enter the known distance A pixel ratio will now be calculated and used for all calculations When maximizing the dialog box the image will scale When moving the cursor over the image it is possible to zoom unzoom the image via the scroll button on the mouse Oxygen Opens a dialog box where users may calibrate convert the input signal from the DAQ S instrument to air sat C The calibration is a 2 point calibration Place the tip of the probe connected as input IN A in a known solution and wait for it to stabilize Press Lock LO and write the air sat temperature value in the LO calibration field Now place the tip of the oxygen probe in a second known solution and wait for it to stabilize Press Lock HI and write the air sat C value in the HI field Use a gt gt 20 difference between the two calibration solutions to maximize the accuracy of oxygen temperature measurements Finally choose if the input should be used as input for INCR or DECR zone Do the same for input IN B 2 3 0 Log Menu Start logging data Opens a note field where users may write notes for the experiment Then a file prompt opens and user must choose a destination for the data file When pressing OK data logging starts Stop logging data Stops logging data
7. to the data file and closes it 5 3 6 Help Menu About This menu will open a dialog box showing the version number of the ShuttleSoft software and contact information for Loligo Systems Page 31 of 34 6 TroubleShooting 6 1 DAQ S connection failure Screen 23 Error 201003 occurred at DAQmx Create Channel D0 Digital Qutput vi i Possible reason s Measurements Device cannot be accessed Possible Causes Device is no longer present in the system Device is not powered Device is powered but was temporarily without power Device is damaged Ensure the device is properly connected and powered Turn the computer off and on again IF vou suspect that Fhe dewire ic damaned conkarct Mational Theke rnent at The error shown on Screen 23 occurs when the NI USB 6008 DAQ card has no properly connection to the PC This error can occur in 2 ways Name is not devi To change the device name open the NI program called Measurement amp Automation look for desktop icon The following example on Screen 24 shows a simulated device named as dev1 The NI USB 6008 DAQ card is registered as dev2 Page 32 of 34 Screen 24 NI USB 6210 Dev1 Measurement amp Automation Explorer File Edit View Tools Help Configuration D Hide Help a Q My System F z ie m Data Neighborhood pack fez Al a Devices and Interfaces aa NI DAQmx Devices NI ARNI USB 6210 Devi DAQmx
8. B controllers of your computer system Hardware Check This directory contains a program that checks if your hardware is compatible with this driver Feadite This directory contains text files with the latest information regarding the installation and the uEye driver Seige This directory contains the installation program for the uEye driver setup exe Please note SOU can choose not to delete the Installation files atter the Setup process Bact Next gt Cancel 18 Select unpack and remove and then click Next Screen 13 ai uEye InstallShield Wizard Remove Installation Files Do you want bo remove the Files used to perform the installation f Unpack the files used to perform the installation to a temporary location and automatically remove these files after the setup is completed Select this option if you are not planning to tun the setup again later Unpack the files used to perform the installation to the location specified below and dont remove these files after the setup is completed Select this option if you are planning to run the setup again at a later time IF the specited folder location does nat exist twill be created automatically Save files in folder To continue click Next lt Back Cancel Page 10 of 34 19 Wait for the files to be unpacked The setup screen will appear when done Screen 14 ES uEye SDK 3 30 Webstart Welcome to the installation program For the
9. Device aA NI USB 6008 Dev2 Basics PXI PXI System Unidentified H Serial amp Parallel 44 Scales 6 Software What do you want a i IVI Drivers to do Q Remote Systems Run the N EDAQmx Test Panels Remove the device v View or change device properties Calibration Right click with mouse on the devi and choose rename or press F2 Change the name to something different than devi In this example the name is changed to devisimu see Screen 25 Screen 25 NI USB 6210 Dev1simu Measurement amp Automation Explorer File Edit View Tools Help Configuration Properties X Delete Zj Self Test A Test Panels fJ Reset Device m Create Task esil gt Hide Help a Q My System TEn w Data Neighborhood Name value sack El Al ig Devices and Interfaces 0x0 ii NI DAQmx Devices NI w DAQmx Device g NI USB 6008 Dev2 i es Basics PXI PXI System Unidentified wy Serial amp Parallel H a Scales el ines What do you want IVI Drivers to do E E Remote Systems Run the NI DAQmx Test Panels gt Remove the device v View or change device properties S Calibration Now change the device name of the NI USB 6008 DAQ card to devi The DAQ S instrument should now work properly with ShuttleSoft Page 33 of 34 Screen 26 NI USB 6008 Dev1 Measurement amp Automation Explorer TABS File E
10. E T ET T E E A 4 4 OXV GOIN rasne eir e EN O a A AOR 5 USINO SNUESON irori er O A O EO OA ai 5 1 Start UD morne aaen a a 5 2 Newexpenmen eiun a a a a ee Do Detail oser a A E A S 5 3 1 GONG lal tad ccsiansiencnaedcasaaied Games a eae ees 5 3 2 PICHON stato et ioe eee ete a urea ale I SERINO MENU seee saa ea aan at iis 5 3 4 Calpration Men sssusa een hadad peter seas 51905 OG MGI i Avaidan towered ametuer Lala euanetanetmmmmerenmucaces 5 3 6 HEID MENU tater t Ar akan a O hee tale k aD nate cau Aaa eases DAQ S connection failure asssssssussnsnesnnnansnnnnnnnnunnnennnunnnnnne RelayS UNnESDONSIVE paiesi iara a a a uEye camera connection failUre s sessssssssersesesussnnenesnsrenrenne Page 2 of 34 3 Installation procedure 3 1 General e ShuttleSoft is a piece of PC software for Shuttle Box experiments e ShuttleSoft require Windows XP or Vista running on a PC with an Intel Pentium IV processor of 1 GHz and 1 GB RAM or better We recommend monitors with a minimum resolution of 1024x768 pixels e ShuttleSoft requires a mouse with a scroll button e Three free USB ports are required on your PC One for connecting the DAQ S instrument one for the digital video camera and one for the dongle e If using WTW COND 3310 instruments for pref salinity experiment two additional USB ports are required e The ShuttleSoft software finds the position of the experiment animal on the principle of contrast between the object and t
11. USER MANUAL ShuttleSoft 1 List of parts O O ShuttleSoft installation CD USB hardkey dongle LabView Vison runtime license uEYE USB camera C mount lens USB cable Tripod adapter Bracket Software CD DAQ S instrument Adapter cables qty 4 DO SET for oxygen control qty 2 OR TMP SET for temperature control qty 2 OR pump for salinity control qty 4 OXY REG for oxygen measurements gty 2 OR TMP REG for temperature measurements qty 2 OR WTW COND 3310 instruments for salinity measurements gty 2 Flow though probe vessel qty 2 User manual Page 1 of 34 2 Contents 1 List of parts 6 TroubleShooting 6 1 6 2 6 3 Zi CONCERNS raaka A AA EA AAE AEEA 3 Installation procedure sssssssssssssssssnssnssssennsusenesnnnesnsnesnsreennee Sl Gene ralera nA eae ein A A AEE AA ANEA 3 2 ShuttleSoft software for WiNdOWS sssssssssssssssrssrssresrerrsrrrsnns 5 9 CONNECTION overview SAIINILY cece cece eeeeeeee eee eeeeeeeeeeeneas 3 4 Connection Overview temperature cece ccccee eee e eee eeeeeeeeeeeenaes 3 5 CONNECTION overview OXYGEN ss sirserisrsisininirirarinssdasarnrdisaninanana 3 6 WIEY CU SB Camel ai seeni rarna Oa E E TA 3 7 DAO SANS TUNEN sorori eana tacameaeaniemec Aaa 3 8 WTW 3310 Cond instrument salinity ONly ccc eee eeeeees 4 EX Penlmene SCLIUD isinen e O EE 4 1 TANK score totrarereticers erne EER E TE TE ETETA 4 2 S n a E A 4 3 Temperature man
12. d 70 in DECR zone Delta is set to 15 Now if the animal enters the DECR zone ShuttleSoft will start decreasing the oxygen value in this zone When the oxygen saturation drops below 65 and Delta thus exceeds a value of 15 air sat ShuttleSoft will also start decreasing oxygen saturation in the INCR zone to maintain a maximum difference of 15 air sat Once the Delta value is lt 15 air sat the bubbling of nitrogen gas will stop again Hysteresis Use a hysteresis value greater than O to ensure that the relay does not flicker on the sharp edge of the Delta value since this might cause a malfunctioning of the solenoid valve The hysteresis value will also determine upper and lower limits maintained by the system while the animal is in OFF zone Example Oxygen saturation is 80 in INCR zone and 70 in DECR zone Hysteresis is set to 1 The animal enters OFF zone between the two tanks and stays there fora while Due to equilibration with the atmosphere oxygen values will rise in both tanks Once the oxygen saturation in DECR zone reaches 71 ShuttleSoft will activate a relay to open the valve blowing nitrogen into this tank until saturation is back to 70 Mask Use this button to toggle between unmodified video images and masked images Binary Use this button to toggle between unmodified video images and thresholded images Trace Use this button to enable disable a trailing trace from being shown behind the moving animal and set the d
13. dit view Tools Help Configuration x Zb Self Test Fh Test Panels fJ Reset Device Mf Create Task gt 5 Hide Help 9 My System zr Data Neighborhood Name value Back fez A y Devices and Interfaces O0x13D8760 ga NI DAQmx Devices NI DAQmx Device PX PXI System Unidentified Basics Serial amp Parallel H a Scales 6J Software What do you want 4 i IVI Drivers to do g Remote Systems gt Run the NI DAQmx Test Panels gt Remove the device v View or change device properties Another USB device was improperly removed If an USB device like a USB memory stick is not properly removed while ShuttleSoft is running there can be a connection problem with the DAQ S instrument To solve this problem close ShuttleSoft Then disconnect the USB cable between the PC and the DAQ S instrument Wait 30 seconds Then connect the cable and wait When the green diode on the front side of the DAQ S instrument is flashing the connection is re established ShuttleSoft can now be opened again 6 2 Relays unresponsive Make sure the DAQ S instrument is connected with a power cable and the Power button is ON 6 3 uEye camera connection failure Make sure that no other program is using the uEYE camera while using it in ShuttleSoft Only one software program at the time can acquire data from the camera Page 34 of 34
14. elivered together with the instrument Before starting ShuttleSoft the WTW instrument serial interface has to be set to secure export of data 4 J Press ENTER in 2 seconds and choose System Then press Reset and choose Yes Then press F1 and choose Reset Press Yes Press the M button several times until the instrument measures in Sal Press the F2 button in 2 seconds Choose Interval and set it to 1 s Now choose Continue and press ENTER The WTW instrument will now start sending data out every second via the serial interface RS232 DO NOT CHANGE ANY SETTINGS LIKE LANGUAGE SEND ID UNIT ETC ELSE THE COMMUNICATION BETWEEN THE INSTRUMENT AND SHUTTLESOFT WILL FAIL Page 19 of 34 4 Experiment set up 4 1 Tank Set up the shuttle tank system as shown on the diagram below Make sure that the flow direction is correct If the flow into the shuttle tank is too high either place a tube clamp on the output side of each pump hose or lower the position of the buffer tank relative to the Shuttle box tank Recirc pump continous Buffer tank DECR Flow TT Probe vessel DECR Shuttle tank ri Cee Probe vessel INCR Buffer tank INCR Recirc pump continous Page 20 of 34 4 2 Salinity The diagram below shows how to connect buffer tanks with water reservoirs not supplied containing high and low saline water The fittings in the middle of each buffer tank are used as overflows
15. er which camera should b used If no USB camera is found connected to the PC the following box will appear Mo USB camera connected to the PE The next step is that ShuttleSoft looks for a DAQ S instrument connected to the PC and is named devi If a DAQ S instrument is connected to the PC but ShuttleSoft has problems finding it please look in the chapter TroubleShooting If ShuttleSoft has found the DAQ S instrument the initialization is done and images from the camera are now seen on the screen The user can now draw a mask avoid analysis of areas outside Shuttle Box INCR and DECR zones calibrate pixels into units of measure and or calibrate the input signals Finally the user can start logging experiment data to an Excel file To zoom a picture place the mouse over the picture and use the scroll button to zoom If ShuttleSoft is enlarged the buttons and graph will remain at the same size but the image is enlarged Page 26 of 34 5 2 New experiment Click Binary and use the vertical threshold bars to find the animal inside the Shuttle Box Start by choosing a narrow range short distance between the 2 bars and then use the right hand side slider to scan the image for your animal If necessary use the filter options found in Settings gt Filter to remove irrelevant pixels in the picture When done click Binary again to view unmodified images To avoid tracking any moving objects outside the Shuttle Box click Settings gt Ma
16. finitions As used in this Agreement the following terms have the following meanings Ww accept the License Agreement s 91 do not accept the License Agreement s 5 Click Next Page 5 of 34 On Screen 5 the status of the installation is shown The installation may take a while Screen 5 f ShuttleSoft Overall Progress Currently installing ShuttleSoft Part 1 of 1 Copying new files Screen 6 1 ShuttleSoft Installation Complete The installer has finished updating your system Some of the installed products require activation Activate these products now 6 Click Next to end installation of ShuttleSoft and activate the product This activation requires an internet connection Page 6 of 34 7 Choose automatic activation and click Next to activate the software Screen 7 Ml Activation Wizard Welcome to the National Instruments Activation Wizard Complete the Activation Wizard to activate your product Activation is the process of acquiring and applying an activation code Automatically activate through a secure Internet connection Use a Web browser email client telephone or Fax machine to acquire an activation code O Apply a 20 character activation code previously acquired For this computer Understanding the National Instruments Software License Agreement How can I move an activated product to a different com uber 8 Enter the serial number found on the LabView Vison run
17. he surroundings frame by frame e The software determines if the animal is in the INCREASING part of the Shuttle box tank part or DECREASING This is defined by the user e The ShuttleSoft software activates and deactivates solenoid valves pumps connected to digital relays on the DAQ S instrument This way water quality can be controlled automatically depending on animal real time positions e The software will save all input data and calculated values to an Excel data file Page 3 of 34 3 2 ShuttleSoft software for Windows The following steps will explain how to install ShuttleSoft on your computer 1 Insert the CD labelled ShuttleSoft and wait until you see Screen 1 If you do NOT see the screen browse the CD and double click on the icon labelled ShuttleSoft exe Screen 1 WinZip Self Extractor ShuttleSoft 1 exe WA Unzipping ShuttleSoft by Loligo Systems me Setup SS Unzipping rocSetupL A T mst 2 Click Next to start installation of ShuttleSoft Screen 2 1 ShuttleSoft Welcome to the ShuttleSoft installer Itis strongly recommended that vou exit all programe before running this installer Applications that run in the background such as virus scanning y utilities might cause the installer to take longer than average to complete Please wait while the installer initializes Cancel Page 4 of 34 3 Select destination directory for ShuttleSoft and LabView drivers and then press Next Screen 3
18. nager Install a quick launch shortcut to uE ype Demo and uEve Camera Manager Read the latest information about this driver release Whata ew tet 28 Select Click Finish to end the installation of the uEye software package 29 Remove the CD labelled ShuttleSoft from your CD drive 30 Restart the Computer You have now finished the installation of all the software Page 12 of 34 ly The following diagram shows how to connect the instruments and devices used for running a preference salinity experiment ini l ion overview sa 3 3 Connect a qgeo Jajdepy E 3jqeo dwnd 3 9489 gSA es A APN puo es awe FAWN gt y 493d OTEE puop Y23dqd SS A qOld qoud YONI OTEE PUOCD AVWAIIONpuoy YONI PSSA VQ0id J a aqoid See eee eee eee s Ova a ny ea tsa cc ca ca ee ee ee ee ee ee ee ee ee ea ee ees Se Se Page 13 of 34 3 4 Connection overview temperature The following diagram shows how to connect the instruments and devices used for running a preference temperature experiment YA I SS A qOld Y NI I SS A qOld seag ace eee a eqoid aqoid snzes dw L snzes dw L t JY dwnd 3u dwnd X23 1 z jeue aunjyessdweay YONI 4AezAjeue ainjesodwsay esa we JAN A N S Z au dwnd T ay dung ajqeo duing 2919289 gSN q89 CCC 91929 1 zdepy Page 14 of 34 on overview oxygen
19. og box where users may create a mask to avoid analysis outside areas of interest Use either one of the upper buttons to draw a closed figure in the image Then decide if you want to include or exclude the enclosed area by pressing either one of the two upper buttons in the lower group of buttons Below there is a button to undo the last step and one to clear the entire mask and start over Also it is possible to invert the drawn mask Created masks can be saved or loaded as bitmap files When maximizing the dialog box the image will scale When moving the mouse over the camera it is possible to zoom unzoom the image via the scroll button on the mouse Zones Opens a dialog box where users may define the INCR and the DECR zone Start by drawing a closed figure Then decide if the closed figure should be used as INCR or DECRS zone by clicking either on the I button or the D button Then draw a next closed figure and choose this as the missing zone Drawn closed figures can be saved or loaded as bitmap files When maximizing the dialog box the image will scale When moving the mouse over the camera it is possible to zoom unzoom the image via the scroll button on the mouse Camera Opens a dialog box where users may set the video settings of the camera Filter Opens a dialog box where user may set the filter options Page 30 of 34 5 3 4 Calibration Menu Calibration Opens a dialog box where users may convert image pixels into mm cm
20. rdware Wizard Windows will search for current and updated software by looking on your computer on the hardware installation CD or on the Windows Update Web site with your permission Read our privacy policy Can Windows connect to Windows Update to search for software Yes this time only Yes now and every time connect a device No not this time Click Next to continue 2 Click on Install the software automatically Recommended and then on Next If Winows cannot find the drivers please insert the WTW disc Found New Hardware Wizard This wizard helps you install software for CP2102 USB to UART Bridge Controller If your hardware came with an installation CD lt 6 or floppy disk insert it now What do you want the wizard to do Install the software automatically Recommended Install from a list or specific location Advanced Click Next to continue 3 The drivers will now be installed when done click Finish Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the software for CP2101 USB Composite Device Click Finish to close the wizard Back Finish 4 Repeat step 1 3 for the second WTW Cond 3310 instrument Page 18 of 34 The WTW 3310 Cond needs to be calibrated before starting an experiment Please follow the instructions in the WTW user manual of the instrument The user manual can be found on the CD d
21. sit the Following Web site ni compactivate I want to register my NI hardware Page 8 of 34 11 Now install drivers for the hardkey dongle When Screen 11 appears click Next Screen 11 2 WIBU KEY Setup This program will install WIBU RE Y Software on your E Welcome to the WIBLU REY Software Setup program computer lt is strongly recommended that you exit all Windows programs and the control panel before running this Setup program Click sCancele to quit Setup and then clase any programs you have running Click Nexte to continue with the Setup program Version 5 20b Cancel 12 Select supported language and click Next 13 Click Next without changing any options 14 Click Next again after you have accepted the summary of the installation 15 When installation is done click Next again 16 Click Finish to end installation of the WiBu drivers Page 9 of 34 17 Now drivers for the uEye USB camera will be installed When Screen 12 appears click Next Screen 12 i uEye InstallShield Wizard Welcome to the self extracting uEye installation WEye 33000 exe is a self extracting archive The files will be saved in the folder you are asked to enter The detant tolder will be TEMP WEWE_ 33000 lt TEMP it a temporary directory selected by the system The following directories wil be created in the specified folder CheckUSa This directory containe a program that show s all US
22. sk to draw a mask excluding areas for analysis When done click OK Now click Calibration gt Pixel and use the mouse to draw a line along an axis of a known distance in the image Then enter the distance and unit for the line The pixel to meter ratio is now calculated Click OK when done Now ShuttleSoft will find the position of the animal and calculate its velocity and the distance moved Click Settings gt Zones and draw two zones one for each of the two Shuttle Box tanks In the red zone INCR oxygen temperature salinity will increase whenever the animal is present In the blue zone DECR oxygen temperature salinity will decrease Any areas outside the two zones will be referred to as OFF zone We recommend leaving a narrow space between the two user defined zones in order to avoid errors or misinterpretation associated with animals taking positions in the pass way between the two tanks for prolonged periods of time When done click OK For every second ShuttleSoft will now determine the animals x y 2D zone position and log how often it enters a new one Click Calibration gt Oxygen Temperature Salinity to do a two point calibration converting analog inputs on channels IN A and IN B into air saturation or C NB If using WTW Cond 3310 this is not necessary Refer the OXY REG TMP REG WTW Cond 3310 user manual for further instructions on how to calibrate or service probes Then decide if input IN A should be used for monitoring ox
23. t O saturation temperature salinity that the animal is exposed to INCR visits This field indicates how often the object has entered the INCR zone This value will be set to 0 when user starts logging DECR visits This field indicates how often the object has entered the DECR zone This value will be set to 0 when user starts logging Distance In this field the distance moved by the object is calculated The unit of measure can be set via the menu for pixel to meter calibration This value will be set to 0 when user Starts logging Velocity In this field the velocity of the object is calculated once per second Please note that the object has to move at least a half pixel length from frame to frame to be obtained as a movement Threshold bars Use the bars to set the threshold used to find the binary picture On the left bar it is possible to set the range of the threshold values The right bar is to move both sliders Example The two sliders on the left are set to 40 and 70 and the red indication is between them Every pixel that has a value between 40 to 70 will be coloured red and all other pixels will be coloured black The slider on the right bar will stay on 55 Now the user moves the slider to 110 The red indication on the left bar will now be between 95 and 125 Page 28 of 34 Delta Use this field to set the maximum difference in oxygen saturation between the 2 zones Example Oxygen saturation is 80 in INCR zone an
24. time license card Then click on Next Screen 8 HI Activation Wizard Activating LabVIEW 68 2 Development System Enter the serial number supplied with your product Serial Number The serial number can be Found on your product s Certificate of Ownership or packaging Page 7 of 34 9 Fill out the fields with your information when done click Next Screen 9 NI Activation Wizard Activating LabVIEW 6 2 Development System The Following information is required to activate your product First Mame Last Mame Organization Ei Jessen Loligo Systems ApS Register my product to Facilitate technical support and ensure I receive important updates From National Instruments Country Denmark Address i Agro Business Park Address cont NES sales City State Postal Code te Sd E Email Phone Fax ej loligosystems com 45859992546 45859992599 National Instruments Product Activation Privacy Statement 10 If activation was successful Screen 10 appears Click on Finish to end the activation Screen 10 NI Activation Wizard Activating LabVIEW 8 2 Development System Product Successfully Activated The Following product has been successfully activated You may need to restart the associated product in order to use any new Functionality Lab IEW 8 2 Full Development System with MathScript IF vou experience any problems or have any questions or feedback regarding product activation vi
25. tomatically 2 Click on Install the software automatically Recommended and then on Next Found New Hardware Wizard This wizard helps you install software for USB 621x Loader if your hardware came with an installation CD lt 4 or floppy disk insert it now What do you want the wizard to do Install the software automatically Recommended Install from a list or specific location Advanced Click Next to continue rE 3 The drivers will now be installed when done click Finish 4 Open the Measurement and Automation Explorer MAX by clicking Start gt All Programs gt National Instruments gt MAX Screen 19 NI DAQmx Devices Measurement amp Automation Explorer gt Hide Help gy My System 5 T a E Data Neighborhood NI DAQmx Devices Device Type Back BE A an Devicassai WA NI USB 6008 Devi Plug in Device ENI DAQmx Devices NI DAQmx Device Basics 4a Scales 6 Software i IVI Drivers What do you want to do 9 Remote Systems Bun the NI DAQmx TestPanels Remove the device View or change device properties 5 If installation was successful the USB 6008 DAQ card is now shown here Page 17 of 34 3 8 WTW 3310 Cond instrument salinity only 1 Connect the WTW Cond 3310 instrument to your PC After a few seconds the software installation will start automatically Found New Hardware Wizard Welcome to the Found New Ha
26. uration of the trailing trace in seconds RE1 This diode indicator gives the relay status for relay 1 On relay 1 the oxygen valve heating pump for the INCR zone must be connected RE2 This diode indicator gives the relay status for relay 2 On relay 2 the nitrogen valve cooling pump for the INCR zone must be connected RE3 This diode indicator gives the relay status for relay 3 On relay 3 the oxygen valve heating pump for the DECR zone must be connected RE4 This diode indicator gives the relay status for relay 4 On relay 4 the oxygen valve cooling pump for the DECR zone must be connected Log This diode will blink while logging When moving the mouse over the diode the actual saving path will be shown Page 29 of 34 Image Here the video images from the camera is shown When maximizing ShuttleSoft on your PC monitor the image will scale accordingly Move your cursor over the image and zoom unzoom by using the scroll button on your mouse Oxygen vs time Temperature vs time graph On this graph oxygen temperature values are shown e g reading from the inputs IN A and IN B The left scale is for the INCR zone red plot and the right scale is for the DECR zone blue plot Interval min Use this field to set the X axis time interval for the graph 5 3 2 File Menu Exit On exit the software will close the camera input and save all settings and then close ShuttleSoft 5 3 3 Settings Menu Mask Opens a dial
27. ygen temperature in the red INCR zone or the blue DECR zone Do the same for IN B When done click OK Now ShuttleSoft will show oxygen temperature salinity values for the zone or tank in which the animal is present During times when the animal is in OFF zone an average between the oxygen temperature salinity values in INCR and DECR zones will be calculated Now set up the difference in oxygen temperature salinity that the system should maintain between the two zones we call it the Delta Hysteresis values Depending on the animals position the valves pumps will be activated deactivated to keep a set difference Delta between the two zones During times when the animal is in the OFF zone the system will maintain the oxygen temperature salinity levels in the two zones recorded prior to entering the OFF zone To start writing experimental data to a file click Log gt Start Then choose a destination for the data file To stop logging click Log gt Stop To exit ShuttleSoft click File gt Exit Page 27 of 34 5 3 Details 5 3 1 General INCR O2 INCR temp INCR sal This field indicates the current O saturation temperature salinity for the INCR zone DECR O2 DECR temp DECR sal This field indicates the current O saturation temperature salinity for the INCR zone Object zone This field indicates whether the object is in INCR DECR or OFF zone Object O2 Object temp Object sal This field indicates the curren
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