Thermo Scientific Varioskan® LUX User Manual
Contents
1. Protocol E 4 Dispense 1 E am Shake 1 Fluorescence 1 P a E E Results ss Dispense 1 T Y Fluorescence 1 Report Protocol Actions Select the protocol actions from the Protocol ribbon Note The software automatically detects the instrument configuration and shows only those actions that are available Figure 36 The Protocol ribbon for adding actions Protocol New Session Skanlt Software 4 0 for Microplate Readers Mov M HM OW WHM v wr Run Plate Out W Well Loop M Pause Run Plate In Absorbance Absorbance Fluorescence Fluorescence Luminescence Luminescence AlphaScreen TRF TRF Spectrum Spectrum Spectrum Spectrum Bil Area Selection ED Dispense Photometry Fluorometry Luminometry AlphaScreen Time resolved Fluorometry Control Actions 34 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific 5 Using Skanlt Software Protocol Table4 Protocol actions and descriptions Action Absorbance Description Measures absorbance Absorbance Spectrum Measures the absorbance spectrum for a wavelength range Fluorescence Measures fluorescence intensity Fluorescence Spectrum Measures the fluorescence spectrum for a wavelength range Luminescence Measures luminescence Luminescence Spectrum Measures the luminescence spectrum for a wavelength range AlphaScreen Measures the AlphaScre2. User Manual Skanlt Software for Microplate Readers Cat No N16044 2015 2015 Thermo Fisher Scientific Inc All rights reserved Thermo Fisher Scientific Inc provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document shall in no way govern or modify any Terms and Conditions of Sale which Terms and Conditions of Sale shall govern all conflicting information between the two documents Release history For Research Use Only Not for use in diagnostic procedures WEEE Compliance This product is required to comply
3. c Remove the tray holder screws 1 and 2 and the transport lock tag Store the lock tag for future use 6 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific Thermo Fisher Scientific 2 Install Varioskan LUX Installation Figure 7 Tray holder screws 1 and 2 and track mechanism bar screws 3 and 4 on the transport lock d Gently push the track mechanism where screws 1 and 2 were fastened into the instrument e Remove screw 3 and loosen screw 4 f Turn the transport lock into its horizontal storage position g Tighten screw 4 Fasten screw 3 back into the same hole from which it was unfastened h Fasten screws 1 and 2 onto the track mechanism bar The transport lock is now in its storage position Figure 8 Screws 1 2 3 and 4 and the released transport lock I UL Install the plate tray and adapter First install the tray then the adapter The tray adapter combinations are individually coded for automatic identification a Gently pull the tray holder out and slide it to the left Thermo Scientific Varioskan Lux User Manual 7 2 Install Varioskan LUX Installation Figure
4. instrument y J Notes S i P J 5 HHH Plate Layout Disp nses 3 i E Protocol Volume ul 50 B E m F E Results Position L de Dispense 1 J Report O F z 7 OL Empty the Dispensers You can use the instrument or software to empty the liquid from the tubes back into the reagent container Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 17 3 Operations Plate Trays and Plate Adapters Figure 22 Dispense button icon 3 a From the instrument Press the Empty button until the liquid flows back b From the software i Click the Empty dispenser s icon to open the Empty window ii Select the dispenser iii Select the volume and speed iv Click Empty Plate Trays and Plate Adapters Plate Trays There are two types of plate trays universal and robotic The universal plate tray is for basic use It is compatible with all plate formats 6 to 1536 well plates Always use a plate adapter with a universal tray A robotic plate tray is for automation use with robots It is compatible with 96 to 1536 well plate formats Use the special robotic plate adapter when using a plate without a lid Remove the plate adapter when using a plate with a lid on a robotic tray When you have installed the plate tray insert a tip priming vessel to the hole on the left edge of the tray Strips of a 96 well strip plate can be used as a priming vessel One strip plate is delivered wit
5. The virtual pipetting section is where you add the samples to the plate You can leave the Plate Layout empty The instrument then measures the whole plate automatically Figure 32 The Pipette content section left and virtual pipetting section right in the Plate Layout Plate template ANSI SBS Standard 96 well M lt Pipette content i Name Plate 1 Remove 5 Sample type v t 1 2 3 4 5 6 c F7 i S Blank L E Standard E em A Control El O Unknown El Sample name B Auto O List Prefix Std C m Replicates Concentrations use specific blank D C Sample groups Define the Samples for the Plate 1 Click Plate Layout in the Session tree 32 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific 5 Using Skanlt Software Protocol 2 Select the plate template from the drop down list 3 Select the Sample type and sample properties 4 Click the plate wells with the virtual pipette your cursor to add the samples Tip You can add multiple samples at a time by dragging the pipette across the wells To clear or edit a well right click on the well Figure 33 Protocol Example To add a series of standard samples concentrations 5 10 50 100 and 500 ug mL with two replicates side by side select the pipette content parameters as shown below and add samples to the plate by painting the wells with the virtual pipette Pla
6. and needs two persons to lift Take into account the weight safety and instrument requirements when deciding where to place the instrument Installation For detailed environmental requirements see the Thermo Scientific Varioskan LUX Technical Manual IMPORTANT Do not touch or loosen any screws or parts other than those specified in the instructions Place the instrument on a sturdy laboratory bench that can take the weight of the instrument Installation order 1 Release the tray holder transport lock Install the tray and adapter Set up the dispensers A WO N Connect the power supply cable and the USB cable 5 Install SkanIt Software Installation Steps 1 Release the tray holder transport lock a Lift up the dispenser sliding cover Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 5 2 Install Varioskan LUX Installation Figure 5 Dispenser sliding cover 1 b Remove the front panel by pulling the two bolts one on each side at the bottom sideways then by turning the bottom of the cover 30 and lifting it off Figure 6 Side bolts 1 front panel 2 The metallic transport lock support is fastened with four screws Two screws 1 and 2 are fastened to the tray holder and two screws 3 and 4 are fastened to the track mechanism bar
7. A MOMO E Report M Sam ple groups Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 27 Install Skanlt Software Select the Software Language Task Ribbon The actions in the task ribbon are linked to the section you have selected in the Session tree When you select Plate Layout Protocol Results or Report in the Session tree the relevant task ribbon opens The task ribbon shows the actions you can select Figure 29 The Protocol task ribbon New Session Skanlt Software 4 0 for amp M Ww Luminescence Luminescence AlphaScreen Spectrum Spectrum Fluorometry B New Session X Q9 n F lj Notes E Measurement order Sl Fr Plate Layout a 3 2 Protocol FE Results m r Report Select the Software Language The default language is English You can change the language to French German Italian Japanese Portuguese Russian Simplified Chinese or Spanish To change the language 1 Click Settings in the Application menu The Settings window opens 2 Select the language from the drop down list under General settings 3 Restart the software to set the new language 28 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific Thermo Fisher Scientific Figure 30 The Settings window O Edit application settings Database Results Saved curves K Factors Instruments Plate Adapters Plate Templates
8. Fisher Scientific Thermo Scientific Varioskan Lux User Manual 19 3 Operations Install LAT Module Filters 1 Click on the Plate template drop down list in the Plate Layout view 2 Select a template that you will use in a measurement The plate templates are automatically associated with the compatible plate adapters When you start a measurement the software checks that the selected plate template and the installed plate adapter are compatible Install LAT Module Filters The LAT module has built in AlphaScreen and TRE excitation filters You need to install the AlphaScreen TRE and luminescence emission filters IMPORTANT Do not touch the surfaces of the filters with bare hands 1 Turn on the instrument and open SkanIt Software 2 Open the dispenser sliding cover and the LAT module cover 3 Select the filter position in SkanIt Software a Click Settings on the application menu b Click Instruments c Click the icon on the right side of the instrument name to open the Edit instrument parameters window d Click the Filter definition tab e Click Add f Selecta free filter position from the filter wheel and add the new filter information g Click Next The filter wheel is now turned to the selected position Figure 24 The Add new filter pop up window Instruments Poli Now 3 m TM USO aros General Dispensers LAT module Report Filter definition Plate Adapters Add Z Ed t X Re
9. KK KK KK KK KK KK KK KK KK 13 Instrument Startup x icsseces do a ead KK KK KK KK KK KK KK KK KK ig 13 Switch the Instrument Off u kk KK KK KK KK KK KK KK KK KK KK k 13 Run the Plate In Out kk kk kk kk kk k kk k kk l el lk lele lee l de ele le lele 14 Set the Instrument Temperature sois KK KK KK KK KK KK KK KK KK KK KK ae 14 Dispense a ERSTE 15 Prime the Dispensers be A AA KK ER KK KK KK KK KK aed 16 Empty the Dispensers 050055054 kk exce ace KK KK KK KK KK KK KK IK KH 17 Plate Trays and Plate Adapters WWW kk kk KK KK KK KK KK KK KK KK KK KK KK IK 18 Ci adapter 18 Plate Adapters AAA IA 19 Install LAT Module Filters ooooooooooomomommo room 20 Gas Control ii o Aone eto cb 21 Set the Gas Empero KK KK KK KK KK KK KK KK KK KK KK KK es 21 Rules tor Safe Use Zl a ye esteso a a E e 22 Install Skanlt Software k kk kk kk KK KK KK KK KK RR KK KK KK KK KA 25 Skanlt SOFtWAN 2 sr a ie 25 Installation Overviews c asics at aa ii 25 Connect the Software to the Instrument 0 0 00 RR RR RR KK KI 26 Thermo Scientific Varioskan Lux User Manual Contents Mam A A de MU d 27 Application Menus 44 bet et kab et hoe MERE EM Re pd EE 27 Session Trees d c ES a an a Reges Qa ew od ets 27 Task RabBOR rre dod tete dolo taa 28 Select the Software Languages bus KK KK KK KK KK KK KK KK KK KK K k 28 Chapter 5 Using Skanlt Software LL kk kK KK ee KK K
10. Scientific Varioskan Lux User Manual Thermo Fisher Scientific Safety and Maintenance This chapter includes the general and instrument safety guidelines and maintenance checklist General Safety Guidelines The instrument is for laboratory research use only Observe proper laboratory safety precautions wear protective clothing and follow approved laboratory safety procedures Follow Good Laboratory Practice GLP to guarantee reliable analyses Instrument Safety Follow the preventative maintenance instructions to keep the instrument in the best condition Go to Maintenance Checklist Observe all safety symbols and markings on the instrument Do not open any covers except the dispenser sliding cover or measurement chamber door when the instrument is plugged in a power source Do not open the measurement chamber door manually when the instrument is in operation LED indicator orange Do not push the plate tray in manually unless the instrument is switched off Do not force a microplate into the instrument Instrument Requirements Check the instrument requirements before you install it Table 6 Instrument requirements Operating conditions 10 C to 40 C Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Indoor use only Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 43 6 Safety and Maintenance Instrumen
11. measurement The software indicates the action it is running 4 Click the action under Results to watch the measurement results during the run If you need to stop the run click Abort The results measured up to that point are saved Figure 39 An Absorbance Spectrum measurement LAS E Home vien MH Y B HM Absorbance Absorbance Luminescence Luminescence Spectrum Spectrum Ww E OKineticLoop zm Shake F Run Plate Out Wi Well Loop iil Area Selection 28 Dispense El Example session 1 x Re Te E M Pause Run Plate In Fluorescence Fluorescence Spectrum Spectrum AlphaScreen TRF TRF Notes Plate Layout i amp Protocol C SIMULATOR ux LES simuraroR 3020 Incubator Off i i i i i J d 4 2000 0 x COa Current step Absorbance Spectrum 1 i i i i 1 i i IMPORTANT Do not open the measurement chamber door during a measurement The dispenser sliding cover can be open Results This is where you can view the measurement results and perform calculations You can also export measurement and calculation data to use outside SkanIt Software View the Results 1 Click the measurement step under Results in the Session tree 2 Click the Plate or List tab to view the results 36 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific 5 Usi
12. the absorbance optics Clean the LAT module Clean the LAT base Clean the dispensers e Replace the dispenser tubings Replace the dispensing tip Replace the dispenser syringe Ensure proper shutdown s Service the instrument Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 45 6 Safety and Maintenance Instrument Maintenance 46 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific
13. 0 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific 5 Using Skanlt Software Open an Existing Session Open an Older Session 1 Click Open on the application menu 2 Select the session from the Session Browser window You can use the Advanced search to find a specific session quickly Pin Your Favorite Session Click the recent session to pin it as a favorite session The favorite session then stays on the recent session list Figure 45 n this example the first session under Open recent session is marked as favorite ad Open Create new session Open recent session E Save Example session 1 e R New session Gal Import Varioskan LUX cian El lal al Export i O Other instrument types a B Settings Information X Exit Export a Session To copy a session or multiple sessions from one SkanIt Software database to another you need to export them first Exporting a session creates a file with an ska extension which you can import to another PC with SkanIt Software installed You cannot open the exported session outside SkanIt Software 1 Open the application menu 2 Click Export The Export session window opens Figure 46 The selected sessions and export folder Select session s EXE __ _ L L LLLLLLLLLLLLLLLLLLLLLLLLjL va TT ml Demo Sessions gt 7 ill Thermo Scientific M EL Example session 1 M El Example session
14. 2 M El Example session 3 Export to Browse 3 Check the session s you want to export 4 Click Browse to select the Windows folder you want to export the session s to 5 Select the file name in the Save As window and click Save Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 41 5 Using Skanlt Software Open an Existing Session 6 Click Ok Import a Session You can import a session or multiple sessions that have been exported with SkanIt Software Files that can be imported have an ska extension 1 Open the application menu 2 Click Import The Import session window opens Do po Click Next Browse to the file location Select the file and click Open 6 Select the session s you want to import and click Finish Figure 47 The session selection in the Import data window amp Import sessions x Select location mes nca xanan Select folder Definitions done sc PIE Ea Sessions to import ET T Session Varioskan LUX Session Varioskan LUX Session Varioskan LUX V uDrop Plate DNA concentra V uDrop Plate DNA concent V uDrop Plate DNA con Click the finish button to import data from the selected file to database lt Back Finish S7 7 The imported session opens The software adds the imported session s to the Open recent session list under New amp Recent 42 Thermo
15. 9 Close up of the tray holder Guide pins 1 screw fix hole 2 contact pins 3 lever opening bar 4 b Install the tray into the tray holder Make sure you first push the positioning lever to the left of the lever opening bar Make sure the two guide pins located on both sides of the tray holder are inserted into the tray Note Install the universal tray without the adapter c Fasten the tray to the tray holder by the screw fix by turning the key clockwise Figure 10 Tray and tray holder 8 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific 2 Install Varioskan LUX Installation Figure 11 Close up of the lever opening bar 1 and positioning lever 2 behind the plate tray 3 d Choose the detachable adapter according to the plate type you are using Figure 12 Detachable adapter for a 96 well plate T VoM 000000000000 000000000000 000000000000 9000000000000 9000000000000 9000000000000 9000000000000 9000000000000 uum n e e Place the adapter at the very bottom of the tray and make sure it is level It may be a tight fit A click sound indicates a successful installation Note The adapter will not go to the bottom of the tray if the positioning le
16. AUTION notice is accompanied by an appropriate CAUTION symbol IMPORTANT Highlights information necessary to prevent damage to software loss of data or invalid test results or may contain information that is critical for optimal performance of the system Note Highlights information of general interest Tip Highlights helpful information that can make a task easier Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual Preface Gontacting Us For the latest information on products and services visit our website at www thermoscientific com platereaders ii Thermo Scientific Varioskan amp Lux User Manual Thermo Fisher Scientific Contents Chapter 1 Chapter 2 Chapter 3 Chapter 4 Thermo Fisher Scientific diri EET i About This Guide s k sw lesse i Related Documentation o oooooooorrror KK KK KK KK KK KK i Safety and Special NOE etica brasero a d due aos i ewi lwlratbty e e ee eee e _ _ _ errrpprrprmm ii Introduction to Varioskan LUX 0 00 A A KK KK RR RR KRE RR KK 1 Intended Us ii DD DES HH E ERO amps 2 Instrument Lada wil a d LR dre l k wa Win aee ed rp RD n ies 3 Install Varioskan LUX LL LA kk kk kK kK KK KK KK KK KK RR KK KK k 5 Installation n xis ebria N ulan n bd dado exu A d eR dead 5 Installation Steps san AAA AAA as AR Az A ye Wi 5 uniri PPM END 13 Switch the Instrument On WW kk kk kk kk KK
17. IMULATOR_LUX SIMULATOR_3020 X Incubator Off 4 20 0 C O3 CO gt From the Instrument Press the Plate In Out button in the instrument left side to run the plate in or out Figure 17 The Plate In Out button icon Adi Set the Instrument Temperature You can set the instrument temperature up to 45 C from the software 1 When a measurement session is open click the temperature icon above the Start button 2 Check the Temperature box and set the temperature 3 Click OK The software shows both the current and target temperatures until the target temperature is reached Tip You can also set the temperature from application menu gt Settings gt Instruments gt General gt Use instrument temperature Note The instrument has no cooling system 14 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific 3 Operations Dispense Dispense You can use two dispensers in the instrument for automatic reagent addition to a microplate Dispensing Tips There are two dispensing tips 1 Tip 0 40 0 40 mm is the default tip and used for volumes gt 5j l 2 Tip 0 25 0 25 mm is for small volumes ranging from 1 to 20yl Note If you change the tip size set the right size also in SkanIt Software Go to application menu gt Settings gt Instruments gt Dispensers gt Tip size Figure 18 Tip 0 25 1 and tip 0 40 2 d X A P y LT A
18. K kk KK KK kk kk kk 31 SESSIONS A Vaeeecateeete tcd etr d lobe e b es 31 Session StrUGEUEE ciii ebbe Abe deb bie en ka y a ca 31 BE Va DD A poa Ge 32 Drot c k sti nos quet O ae han D OK O eee de 33 Protocol Actions ida dik e DLR ERS 34 Start a Measurement lesser kk KK kk 36 A to og 36 Calculations na dee Lah pa ha teed onan HC RO pbk SUO 37 Calculation Actions os Kay eae ehini are ainina aneha a e eai 38 ReDOEb A ados 39 Sd qeu o e 40 Openan Existing Sessions epe at a pic eor Sew Re 98 40 Chapter6 Safety and Maintenance 0 cece eee eee eee KK KK kk kk I 43 General Safety Guidelines la crouse oco wanes AGGERE KK KK KK KK ie 43 Instrument Saleen Aya s lak Ak A eB WURA A 43 Instrument Requirements ro kK AM KK KK KK KK KK KK KK ees SER Moa 43 Safety Specifications o e A KK KK KK KK kk KK kk kk kk kk k 44 Emergency Situations keyl kh leye AM aeg c Weye n b keya lela n 44 Instrument Maintenance 1 0 kk KK KK KK KK KK KK KK kK KK kk KK KK kk kK kk k 45 Maintenance Checklist lt ir da Ma ee ch y aut e y nk e anta 45 iv Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific Introduction to Varioskan LUX Thermo Scientific Varioskan LUX is a modular multi technology microplate reader Varioskan LUX is controlled by Thermo Scientific SkanIt Software for Microplate Readers End point kinetic
19. Security General settings Language English gt fV Check plate and tip prime vessel before session execution 7 Leave plate in after session execution V Optical response compensation V Generate instrument communication log Note The communication log will be generated to CAProgramData Thermo MIP Readers Logs You must restart the application for the change to take effect Do not leave the feature on for prolonged periods to prevent the log file from growing Laboratory information Laboratory name Street address Phone number Email address Email reporting 4 Install Skanlt Software Select the Software Language f Email server name Sender address Colors Software color theme Storm H Thermo Scientific Varioskan Lux User Manual 29 Install Skanlt Software Select the Software Language 30 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific Using Skanlt Software This chapter describes the Session tree which is the main part of the software user interface You will find information on how to create sessions view and export measurement results perform calculations and create data reports The general outline for using the software is 1 Create a new session or open an existing one 2 Define the plate layout and protocol 3 Start the session 4 View the results and perform calculations 5 Create result report and ex
20. able to the mains power supply connector on the back panel If you need to use a different type of mains supply cable other than the one supplied use only cables certified by the local authorities b Connect the instrument mains supply cable to a correctly installed line power outlet that has a protective conductor that is grounded 10 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific 2 Install Varioskan LUX Installation Figure 15 USB connector 1 and mains power supply connector 2 c Connect the instrument to the PC using a USB cable 5 Install SkanIt Software For installation instructions go to Install SkanIt Software Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 11 2 Install Varioskan LUX Installation 12 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific Operations This chapter describes the instrument preparation steps you can take before you start a measurement After you have installed the instrument switch it on and start SkanIt Software The software finds the instrument automatically If you have not installed the software go to Installation Overview Do do not operate the instrument when it is disassembled Switch the Instrument On The power switch is on the left side panel of the instrument Switch it to the on position The LED indicator on the front cover of the instrument i
21. asurement data is the source data for the Blank Subtraction calculation and Blank Subtraction data the source for Standard Curve rr Notes Plate Layout E Protocol Session tree Y Fluorescence 1 rr E Results Y Fluorescence 1 zt Report L 5 Summary Calculation Actions Blank Subtraction 1 LA Standard Curve 1 Select the calculation actions from the Results ribbon Figure 42 The Results ribbon for adding calculations Be BL EE EE Add Calculations B GB 1 XDilution Factor a x BH Normalization Blank Average SD Basic Subtraction cV Calculation J Pathlength Correction Basic LAE Results New Session Skanlt Software 4 0 for Microplate Readers e e P amd pe j Y Quality Control us Graph fx Custom Formula Standard Classification Curve Curves Reduction ji Processing Extra Tools Table 5 Calculation actions and descriptions Action Blank Subtraction Description Subtracts the average blank value from all of the samples Average SD CV Calculates the average standard deviation SD and the coefficient of variation CV of sample replicates Basic Calculation Performs simple calculations such as subtractions multiplications and divisions Dilution Factor Multiplies the results of the unknown samples by dilution factors defined in the plate layout Normalization Pathlength Correction Normalizes the data of a
22. ck 1 or Microsoft Windows 7 with Service Pack Windows 8 1 1 or 64 bit edition of Microsoft Windows 8 1 Disk space 14 GB free disk space Solid state drive with 14 GB free disk space Processor Dual core processor 2 GHz or faster Quad core or dual core with four logical processors 2 GHz or faster Memory 4 GB RAM 8 GB RAM USB port available 1 one 1 one CD ROM drive 1 one 1 one Graphics Processing Unit Dedicated Dedicated Monitor SXGA monitor with 1280 x 1024 SXGA monitor with 1280 x resolution 1024 resolution Note We strongly recommend using a computer that fulfills the recommended requirements especially if you process sessions with more than total of 150 000 individual measurements or with complex calculations Installation Process 1 Insert the installation CD into the CD ROM drive 2 Follow the installation instructions 3 The installation ends with a Completed message To open the software click the SkanIt Software shortcut on the desktop 4 Enter the serial number that is on the installation CD 5 Enter the installation code you received after you registered Note You can use SkanIt Software for 30 days without the installation code For detailed installation steps see the Thermo Scientific SkanIt Software Technical Manual Connect the Software to the Instrument Connect the software to the instrument by plugging the USB cable from the PC to the instrument Switch the instrument on and start S
23. e NO r ATA ME Y e AT uf Z A N A A N P L 4 ZL LANN Nw Pd NN II d JA JU e m LL Ps 7 p CQ Y Dispensing Positions F and L The instrument has two dispensing positions F fluorescence and L luminescence Both positions can be used either with dispenser 1 or 2 The instrument recognizes which dispensing position a dispenser head is inserted into Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 15 3 Operations Dispense Figure 19 Dispensers 1 and 2 dispensing positions F and L Table 1 Dispensing positions and the measurement positions they point at Position F Position L Fluorescence top of the well Luminescence Absorbance Fluorescence bottom of the well Spectral TRF TRF Spectral luminescence AlphaScreen To start a measurement at the same time as dispensing place the dispensing head into the dispensing position F or L that points at the correct measurement position see Table 1 above This way there is no delay time between dispensing and measurement which is important in fast kinetic reactions If you use a dispensing position that does not point at the correct measurement position the instrument makes an extra plate movement before the measurement step This may cause minor time delays between dispensing and measurement Prime the Dispensers Before you use dispensers in a measurement session you must prime them Priming dispensers
24. en signal TRF Measures time resolved fluorescence TRF Spectrum Measures time resolved fluorescence spectrum for a wavelength range Kinetic Loop Executes sub steps several times in defined time intervals in a kinetic measurement Well Loop Executes sub steps for as many wells at a time as you have selected as a well count Area Selection Executes sub steps for only part of the defined wells in the plate layout Area definition is not necessary when all of the defined wells in the plate layout are measured Shake Shakes the microplate to mix the liquid in the wells Dispense Dispenses a given volume of liquid into wells Pause Pauses the protocol Run Plate Out In Runs the plate in or out in the middle of a protocol Tip To perform a kinetic measurement add the measurement step as a sub step of the kinetic loop Figure 37 An example of a kinetic absorbance measurement r Notes SEH Plate Layout Protocol L CD Kinetic Loop 1 E Results L Session tree Absorbance 1 Report Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 35 5 Using Skanlt Software Start a Measurement Start a Measurement 1 Click the Start button Figure 38 The Start button 20 0 O2 CO 96 2 Write a name for the session in the Session Name field This step is skipped if you have previously named the session 3 Click Save to start the
25. g is excluded For validation of the entire system it is recommended that Good Laboratory Practice GLP is followed to guarantee reliable analyses 2 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific 1 Introduction to Varioskan LUX Instrument Layout Instrument Layout The front view of the instrument Figure 2 The power switch 1 LED indicator 2 dispenser sliding cover 3 front cover 4 and measurement chamber door 5 A To O The back view of the instrument Figure 3 The cooling fan outlets 1 USB connector 2 mains power supply connector 3 power switch 4 and gas connectors 5 Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 3 1 Introduction to Varioskan LUX Instrument Layout The instrument with the dispenser sliding cover open Figure 4 Plate In Out button 1 LAT module 2 Prime and Empty buttons 3 dispensers 1 and 2 4 reagent bottle holder 5 4 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific EL Install Varioskan LUX This chapter describes how to install the instrument IMPORTANT The instrument weighs 53 60 kg 117 131 lbs
26. h the instrument accessories 18 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific 3 Operations Plate Trays and Plate Adapters Figure 23 Assembly illustration of a universal plate tray tray adapter combination identification 1 tip priming vessel 2 screw fix 3 position calibration hole 4 tip priming vessel holder 5 plate adapter 6 positioning lever 7 plate tray 8 Plate Adapters Plate adapters are used for adapting plates that are of different heights Adapters lift the plates to the optimum height for measuring and dispensing Before you run a measurement check that the correct plate adapter is in the plate tray Choose the adapter based on the plate format you have 96 384 and if you are using a lid or not Table 2 Available plate adapters Adapter no Plate adapter 96 well adapter without lid Supports dispensing 96 well adapter with lid 384 well adapter without lid 384 well adapter with lid Di M AR 2 96 well adapter for PCR plate without lid 48 6 48 well adapter with lid 65 1536 well adapter 10mm without lid 80 6 48 well adapter without lid 126 Robotic tray with adapter without lid 127 Robotic tray without adapter without lid Supplied with the instrument Plate Template and Adapter Association in the Software You need to select the plate template you will use in the measurement Thermo
27. kanIt Software The software finds the instrument automatically 26 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific 4 Install Skanlt Software Main Elements Main Elements The main elements in the software are the application menu the Session tree and the task ribbon When you open the software the application menu opens Application Menu The application menu is for general tasks This is where you create new sessions open saved sessions and access instrument settings Figure 27 The application menu Skanlt Software 4 0 for Microplate Readers Create new session Open recent session New session Varioskan LUX O Other instrument types X Settings Information X bit Session Tree The Session tree is visible when you have created or opened a session This is the main area where you navigate to define what wells to measure to select the protocol view the measurement results perform calculations and to create a result report Figure 28 The Session tree with Plate Layout selected New Session Skanlt Software 4 0 for Microplate Rd Vex g Clear Preview Add all x new Rava i Plate template ANSI SBS Standard 96 well r Pipette content Name Plate 1 Sample type rm 2 2 6 Blank L O Standard O Control C Unknown RH Plate Layout FE Protocol Results Pipette content
28. means filling the tubing with the dispensing liquid The aspirate tube is the input tube which is between the reagent container and syringe The dispense tube is the output tube Figure 20 Prime button icon 9 16 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific 3 Operations Dispense You can start the priming from the software or instrument 1 Insert an empty plate of the same height as the assay plate into the plate tray 2 Place the head of the aspirate tube into the reagent container 3 Hold the dispense head in a waste container IMPORTANT Do not insert the dispensing head into the dispensing position F or L during priming 4 Start the priming a from the instrument Press the Prime button until the liquid flows out b from the software i Click the Prime dispenser s icon to open the Prime window ii Select the dispenser iii Select the volume and speed iv Click Prime 5 Insert the dispensing head in position F or L Dispensing Step in a Measurement Session After priming the dispensers you can run a session which includes dispensing Make sure the dispensing parameters are correct Select the same dispenser 1 or 2 and position F or L for both the software and instrument The instrument automatically checks that the same positions are selected Figure 21 Select the same dispenser 1 or 2 and position F or L in the software as you selected in the
29. ments Kinetic spectral or multipoint results are not included in the result summary You can export any data by selecting the individual result sections to the report Create a Data Report 1 Click Report in the Session tree 2 Check the sections you want to include in the report from the Report sections list Figure 43 The Report pane open with Report sections checked E Example session 1 x lt 7 Notes Select included report sections Y E 5 EH Plate Layout v V Report sections o E HR Protocol y V Results L v Boorescence d v V Fluorescence 1 Plate rq M Blank Subtraction 1 Plat F Results M TE V Standard Curve 1 Plate W Fluorescence 1 L wP Blank Subtraction 1 V Result summary LLY Standard Curve 1 V General information E Em N Session information Repo i V Instrument information E Summary V Protocol parameters V Run log V Layout definitions Automatic export after execution 7 Print to default printer T Send Excel report via email to 7 Save to file Export a Result Report Manually 1 Click the PDE Excel XML or TXT format on the Results ribbon to export the report Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 39 5 Using Skanlt Software Saved Sessions 2 Save the report The report automatically opens in the format you choose Export a Result Report Automatically You can se
30. ments in the user interface For more information about the software see the Thermo Scientific SkanIt Software for Microplate Readers Technical Manual Cat No N16046 The software automatically detects how Varioskan LUX is configured and shows only those features that are available Note Your instrument may not have all of the features presented in this guide Skanlt Software With SkanlIt Software you can Control instrument actions Create measurement sessions and start measurements View measurement results and perform calculations with the data Create comprehensive result reports Print or export result reports in different file formats e g Microsoft Excel Export and import sessions between Skanlt Software databases in different PCs All measurement and calculation data is stored in the SkanIt Software database Installation Overview To install SkanIt Software you need Administrator rights for the PC To register at http www thermoscientific com skanit to receive the installation code by email The installation CD To make sure your PC meets the minimum requirements Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 25 Install Skanlt Software Installation Overview Table 3 PC requirements System Minimum requirements Recommended requirements Supported operating Microsoft Windows 7 with 64 bit edition of Microsoft systems Service Pa
31. move Plate Templates osition Name Installation date Wavelength Security 4 Open the blue filter nest lid on the LAT module 20 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific 3 Operations Gas Control 5 Loosen the filter wheel screw on the selected position 6 Place the filter on a clean dust free surface with the arrow on the side of the filter pointing upwards 7 Use the filter pick up tool to place the filter into the bottom of the filter nest 8 Tighten the filter wheel screw Figure 25 Filter nest 1 and pick up tool 9 Close and fasten the filter nest lid 10 Click Finish Gas Control The optional Integrated gas module allows you to control the CO and or O5 concentration inside the instrument measurement chamber in cell based assays CO concentration range 0 1 2096 QO concentration range 1 21 You need to connect gas bottles to the instrument The O concentration is adjusted by feeding nitrogen N3 into the instrument See the Thermo Scientific Varioskan LUX Technical Manual Cat No N16045 for details on how to connect the gas bottles and use gas control CAUTION Use a carbon dioxide and oxygen sensor in the room where the Integrated gas module is used CAUTION Follow safety procedures when working with gas bottles Set the Gas Atmosphere 1 Click the gas atmosphere icon above the Start button to open the pop up window The
32. ndicates three states green on and ready for operation orange in operation red error If there is no light the power switch is off or the power cable is not plugged in Instrument Startup The instrument performs a set of initialization tests and adjustments It also performs mechanical electrical and optical checks The LED light is orange during this check When the instrument is ready for measurements the LED light turns green and the plate tray is run out If anything fails in the initialization tests or adjustments the LED light turns red Turn the power switch off then on If this does not help contact the authorized technical service Switch the Instrument Off Switch the instrument off after daily use 1 Rinse the dispenser tubing thoroughly with deionized distilled water after each use Leave the fluid pathway filled for storage 2 Run the plate tray in from the software or from the instrument Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 13 3 Operations Run the Plate In Out 3 Switch the instrument off from the on off switch on the left side panel of the instrument Run the Plate In Out You can run the plate tray in or out from the software or instrument From the Software Click on the Run plate in or Run plate out icon below the Start button Figure 16 From left to right under Start Run plate in Run plate out Prime dispenser s Empty dispenser s S
33. ng Skanlt Software Calculations Figure 40 The results of a Fluorometric Spectrum measurement with the Plate view open E Example session 1 x Notes Plate Layout Session tree S Protocol p Fluorescence Spectrum 1 Results Fluorescence Spectrum 1 Report I Export Results to Excel 1 In the Results view click on the Export to Excel tab 2 Save the data Tip You can export the data of several steps into the same file by creating a report You can create result reports in Excel PDF XML and TXT formats Calculations The software has built in calculations that you can use to process data You can add calculations either before or after a measurement You can add several calculations to a measurement and also nest calculations The calculation uses the result data that is directly above it in the Session tree Add a Calculation ha Select the results step in the Session tree that you want to use as the source data for the calculation N Click the calculation action on the Results ribbon The action appears in the Session tree Oo Define the calculation parameters if needed 4 Click the Plate or List tab to view the calculation results 5 Click Save Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 37 5 Using Skanlt Software Calculations Figure 41 In this example the Fluorescence Me
34. not touch filter or optical lens surfaces with bare hands 9 Do not damage the optical system components 22 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific 3 Operations Rules for Safe Use Note 1 cS ao OS A Decontaminate the instrument before removing from the laboratory and before servicing Check the installation and maintenance checklists Keep all of the holes in the plate adapter clean Do not use the instrument if it does not function properly Do not spill fluids in or on the equipment Take the chemical resistance of the dispensers and microplates into account Make sure the microplate or tip priming vessel is not too full o not use any liquids that may cause precipitation or congealing or that contain mechanica Do not liquids that tat that cont h l particles with the automatic dispensers Do not let the dispensers run dry Tip 1 De 2 Empty the priming vessel after use Keep the bottom of the microplates dry to avoid contamination Change the 8 well plate strip 1x8 Thermo Scientific Microtiter Solid Strip Assembly priming vessel when needed Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 23 3 Operations Rules for Safe Use 24 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific Install Skanlt Software This chapter gives an overview of the software installation process and introduces the main ele
35. port data Sessions The information that is needed to define and run an assay is saved in a session With SkanIt Software you can build sessions for your own assays and run or modify ready made sessions Session Structure The Session tree is the main use area in the software The Session tree has five main sections 1 Notes write notes about a session 2 Plate Layout define which wells of the microplate you want to measure 3 Protocol define what you want the instrument to do e g measure shake 4 Results view the measurement results and choose your calculation methods 5 Report create a report of the measurement and calculation results Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 31 5 Using Skanlt Software Plate Layout Figure 31 The Session tree B Notes Plate Layout amp Protocol E Results Report Session tree Create and Save a Session 1 Click the application menu tab 2 Click the New session button under New amp Recent 3 Click Save As or Save in the Home ribbon 4 In the Save as session window select the folder where you want to save the session The sessions are saved in the SkanIt Software database 5 Give the session a name and click Save Plate Layout This is where you tell the software which wells to measure or dispense and what kind of samples you have in the microplate The Pipette content section is where you define the sample properties
36. rmo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 21 3 Operations Rules for Safe Use Figure 26 The gas atmosphere pop up window SIMULATOR_LUX SIMULATOR_3020 X Incubator Off 4 20 0 C O2 CO gt Mi Regulate oxygen Oz level 21 0 a m Regulate carbon dioxide CO level 5 0 e El o cane 2 Set the oxygen and carbon dioxide levels as needed and click OK You can now see the current and target gas concentrations above the Start button Rules for Safe Use CAUTION 1 Do not open optical covers to avoid ultraviolet radiation injury 2 Do not operate the instrument when it is disassembled or exposed 3 Do not use cell or tissue cultures in the device that are not in accordance with the regulations of safety levels L1 L2 and L3 4 Use a carbon dioxide and oxygen sensor in the room where the Integrated gas module is used 5 Follow safety procedures when working with gas bottles IMPORTANT 1 Do not open the chamber door during operation Only authorized personnel can open optical covers Use blind plugs to close empty dispensing positions to avoid stray light Do not wash the electronic parts of the LAT base when you clean the instrument from below Make sure the dispense tubing is properly installed to avoid leakage Do not autoclave any part of the instrument on ws MM Do not loosen or remove screws or parts other than those allowed in the instructions 8 Do
37. sample group to a Bg reference sample The results are shown in percentages Normalizes absorbance measurement data to correspond to a 10 mm pathlength standard cuvette Standard Curve Dose Response Calculates the concentrations of samples based on a standard curve generated from a standard sample series Calculates the concentration at which e g 50 of a measured sample activity is lost ED50 Kinetic 38 Thermo Scientific Varioskan Lux User Manual Offers different kinds of calculations for kinetic data Thermo Fisher Scientific 5 Using Skanlt Software Report Table 5 Calculation actions and descriptions Action Description Spectral Offers different kinds of calculations for spectral data Multipoint Offers different kinds of calculations to reduce multipoint measurement results in each well to one result per well Classification Divides samples into separate categories based on user defined limit values Quality Control Checks the validity of the assay e g against known control samples Custom Formula Lets you create custom calculations Graph Creates graphs from the result data Report You can create a result report including both measurement and calculation data You can export the result report to Excel PDE XML and TXT formats A summary table is automatically created under Report The summary table shows only the measurement and calculation results of end point measure
38. spectral and multipoint measurements can be carried out in the UV Vis NIR range from appropriate microplate formats In fluorescence intensity time resolved fluorescence luminescence and AlphaScreen measurements 6 to 1536 well plates can be used and correspondingly 6 to 384 well plates in absorbance measurements Figure 1 Thermo Scientific Varioskan LUX EA Thermo VABIOSKAN LUX The instrument is always equipped with the following detection technologies e Absorbance Fluorescence intensity FI Depending on the instrument model the following optional detection technologies LAT module may be included Luminescence AlphaScreen Time resolved fluorescence TRE The instrument selects the measurement wavelength either by using filters or monochromators depending on the measurement technology Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 1 1 Introduction to Varioskan LUX Intended Use Monochromators are used in absorbance and fluorescence intensity measurements Filters are used in AlphaScreen and TRF measurements Most luminescence measurements do not require any wavelength selection But if required filters can be used The LAT module has built in excitation filters for TRF and AlphaScreen The TRE and AlphaScreen and luminescence emission filters you need to install yourself Note All measurement technologies except AlphaScreen allow
39. spectral scanning measurements with monochromators The instrument has an incubator for temperature control up to 45 C and a plate shaking capability with orbital shaking mode The instrument can also be equipped with Dispensers up to two for automatic reagent addition e Integrated gas module for controlling the gas atmosphere CO amp O inside the instrument The optical system of the instrument allows you to perform Fluorescence intensity measurements from the top or bottom of the well Luminescence TRF and Alpha technology measurements from the top of the well Absorbance measurements through the well Note Fluorescence measurements from the bottom of the well require an instrument model supporting bottom reading Note Your instrument may not have all of the features presented in this guide As the instrument is modular you can upgrade it with missing features later Intended Use The Varioskan LUX modular multimode reader is used to measure fluorescence intensity FI time resolved fluorescence TRF absorbance luminescence and AlphaScreen from samples in appropriate microplates The instrument also has incubating atmospheric control shaking and reagent dispensing capabilities It is used with an external computer control software The reader is intended for use in research laboratories by professional personnel The multimode reader is not intended for diagnostic use Use for self testin
40. t Safety Mains power supply 100 240 Vac 50 60 Hz nominal Power consumption 200 VA maximum Safety Specifications The safety specifications are also met under the environmental conditions listed in the table below in addition to or in excess of those stated in the operating conditions Table 7 Safety specifications Altitude lt 2000m Temperature 5 C to 40 C Humidity Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 Mains supply fluctuations 10 from nominal Installation category overvoltage II according to IEC 60664 1 category Pollution degree 2 according to IEC 60664 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overrvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply The pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only non conductive pollution such as dust occurs with the exception of occasional conductivi
41. t the software to export the report automatically after the run to a specific destination Before you run a session you need to select the report content and where to export the report 1 Click Report in the Session tree 2 Check the Save to file box in the Automatic export fter execution pane 3 Name the file and click Browse to select the destination folder and the file format 4 Save the session The next time you start the session a report is automatically saved in the destination folder you selected Saved Sessions There are two different kinds of saved sessions a A session that is saved before you have run it A session that you have saved but have not run does not have measurement data You can edit all of the content b A session that has been run A session that has been run is automatically saved You cannot edit the protocol but you can edit all other content A green arrowhead icon indicates a saved session with measurement data Figure 44 A session with measurement data green icon and without measurement data no icon Open recent session saved session with measurement data E saved session with no measurement data Open an Existing Session You can open a recent session or an older session Open a Recent Session 1 Click New amp Recent on the application menu 2 Select a recent session from the Open recent session list The session opens in the Session tree 4
42. te template ANSI SBS Standard 96 well M Pipette content Sample type O Blank Standard O Control O Unknown Name Plate 1 Pipette content MOMO Sample name Auto List Prefix Std mM Replicates Columns Rows iB B LE l N Concentrations O Series Values 5 10 50 100 500 Unit ug mL T Use specific blank T7 Sample groups This is where you define which actions the instrument performs The instrument runs the actions in the order as listed under the protocol Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 33 5 Using Skanlt Software Protocol Figure 34 In this example the instrument first dispenses liquid to the wells then shakes the plate and then measures the fluorescense G E i Notes 5 E Plate Layout E 4 Dispense 1 E zm Shake 1 E Y Fluorescence 1 EJ Results E FO Dispense 1 Y Fluorescence 1 E Report Define a Protocol 1 Click Protocol in the Session tree 2 Select the action from the Protocol ribbon The action appears in the Session tree 3 Define the action parameters e g measurement wavelength To change the order of the actions click the action you want to move then click the small arrowhead to move it up or down Figure 35 To move the action up or down click the arrowhead icon To remove the action click the x mark P F Notes E Plate Layout amp
43. ty caused by condensation Emergency Situations In emergency situations 1 Switch off the instrument 2 Unplug the instrument form the power supply 3 Carry out corrective measures Note Do not disassemble the instrument For more help contact authorized technical service or your local Thermo Fisher Scientific representative 44 Thermo Scientific Varioskan Lux User Manual Thermo Fisher Scientific 6 Safety and Maintenance Instrument Maintenance Instrument Maintenance Clean the instrument after each use 1 Switch the instrument off with the tray out 2 Wipe the surface of the tray with a soft cloth or tissue paper moistened with distilled water mild detergent SDS sodium dodecyl sulfate or a soap solution 3 Disinfect any spilled infectious agents with 70 ethanol or another disinfectant 4 Push the tray back into the instrument 5 Check the dispensers for leaks and correct any problems Maintenance Checklist Table 8 Maintenance checklist Maintenance Daily Weekly Monthly Yearly If required Keep the instrument clean Clean the instrument case and reagent basin Clean the measurement chamber Clean the tray Change the 8 well plate strip priming vessel after 250 tip primings if the priming is 10pl and after 2500 tip primings if the priming volume is 1j l Clean the dispensing base Clean the fluorescence bottom reading optics Clean
44. ver is in the way f Push the plate tray into the instrument g Replace the front cover by inserting the two top door latches into place while turning the cover 30 and then snapping it shut 3 Setup the dispensers a Fit the complete dispensing tube assembly into the left hole of the valve and tighten it finger tight The dispensing tube is used to dispense reagent from the syringe into a microplate Connect the male connector of the dispensing head position sensor to the corresponding female connector above the valve The aspirate tube assembly is factory installed into the right hole of the valve Thermo Fisher Scientific Thermo Scientific Varioskan Lux User Manual 9 2 Install Varioskan LUX Installation Figure 13 Dispenser Male connector of the dispensing head position sensor 1 dispensing tube assembly 2 dispenser syringe and plunger 3 plunger lock screw 4 valve 5 aspirate tube assembly 6 dispensing head 7 dispensing tip 8 b Insert the dispensing heads into their respective head holder slots on the left hand side of the dispenser c Remove the protective cap from the dispensing tip Figure 14 Cap and dispensing tip lt lt p E we Da 4 Connect the power supply cable and the USB cable CAUTION Make sure the power switch is in the off position Do not operate your instrument from a power outlet that has no ground connection a Connect the mains supply c
45. with the European Union s Waste Electrical amp Electronic Equip ment WEEE Directive 2002 96 EC It is marked with the following symbol Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union EU Member State and these companies should dispose of or recycle this product Contact Thermo for further information on Thermo Fisher Scientific s compliance with these Directives and the recyclers in your country Preface About This Guide Thermo Scientific Varioskan LUX is a modular multi technology microplate reader and it is controlled by Thermo Scientific SkanIt Software for Microplate readers This guide gives an overview of the instrument and software installation procedures and describes the main operations and routine maintenance Related Documentation In addition to this guide Thermo Fisher Scientific provides the following electronic documents for Varioskan LUX and SkanIt Software Thermo Scientific Varioskan LUX Technical Manual Cat No N16045 Thermo Scientific Skanlt Software for Microplate Readers Technical Manual Cat No N16046 The software also has a Help Safety and Special Notices Make sure you follow the precautionary statements presented in this guide The safety and other special notices appear in boxes Safety and special notices include the following CAUTION Highlights hazards to humans property or the environment Each C
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