Multipette® plus · Repeater® plus - Operating
Contents
1. ena Dis becomes wes Vestae Beard ef Maragemer 01 08 2011 Panties Cute Evaluate your operating manual www eppendorf com manualfeedback eppendorf In touch with life Your local distributor www eppendorf com worldwide Eppendorf AG 22331 Hamburg Germany Tel 49 40 53801 0 Fax 49 40 538 01 556 E mail eppendorf eppendorf com Eppendorf North America Inc 102 Motor Parkway Hauppauge N Y 11788 5178 USA Tel 1 516 334 7500 Toll free phone 1 800 645 3050 Fax 1 516 334 7506 E mail info eppendorf com Application Support Europe Tel 49 1803 666 789 Preis je nach Tarif im Ausland 9 ct min aus dem dt Festnetz Mobilfunkh chstpreis 42 ct min support eppendorf com North America Tel 1 800 645 3050 E mail techserv eppendorf com Asia Pacific Tel 60 3 8023 6869 E mail support_asiapacific eppendorf com2. 10 Disposal b 10 1 Disposal In case the product is to be disposed of the relevant legal regulations are to be observed Information on the disposal of electrical and electronic devices in the European Community Within the European Community the disposal of electrical devices is regulated by national regulations based on EU Directive 2002 96 EC pertaining to waste electrical and electronic equipment WEEE According to these regulations any devices supplied after August 13 2005 in the business to business sphere to which this product is assigned may no longer be disposed of in municipal or domestic waste They are marked with the following symbol to indicate this As disposal regulations may differ from country to country within the EU please contact your supplier if necessary WARNING Risk of explosion and fire due to overheated accumulators and batteries Do not heat accumulators and batteries to over 80 C and do not throw them into fires Disposing of accumulators and batteries Do not dispose of accumulators and batteries as X household waste Dispose of accumulators and batter ies according to the locally applicable legal regula tions 16 EG Konformit tserkl rung EC Conformity Declaration Dan berv scuta Medial eignet den erage nega At der KASI Tre product nameg ticum MAB Pw Andante of Je ES ang taria ia Te cave r an
3. Multipette plus Repeater plus Operating manual eppendorf Copyright 2012 Eppendorf AG Hamburg No part of this publication may be reproduced without the prior permission of the copyright owner Trademarks Eppendorf Multipette and Repeater Combitip Biopur are registered trademarks of Eppendorf AG Hamburg Germany Registered trademarks are not marked in all cases with TM or 9 in this manual U S Patent No 5 591 408 5 620 660 5 620 661 U S Design Patent No Combitip plus 384 163 384 162 387 426 4981 900 095 07 062012 Multipette plus Repeater plus Operating manual Table of contents 1 User instructions 3 1 1 Using this manual 2 ss s sa n s 3 2 Product description 3 2 1 P ras 3 3 5 3 1 s eek e rape di aan 5 4 Oporation cae sos aa pk 7 4 1 Choos CombitiDiz i2 cen ncm bte 7 4 2 Insert Combitip xeu snc uusha e and akan 8 4 3 Select volume nungen name 8 4 3 1 Filling Combitip and reverse stroke 8 4 3 2 Discard first dispensing step reverse stroke 9 4 4 Boon 9 4 5 Release Combitip 9 4 6 en ecce EE 10 4 7 Empty Battery 10 5 Troubleshooting 11 5 1 General errors u l 11 6 M
4. Description 4981 000 019 Multipette plus 022260201 Repeater plus 8 1 1 Accessories Multipette plus Repeater plus Order No International Order No North America Description Pipette carousel 3115 with 6 pipette holder for Research and Reference International North America 3115 000 003 022444905 Pipettes Multipette plus Repeater plus Varipette adapter 4981 201 006 022260588 for pipette carousel 3115 4980 210 001 022261592 for pipette carousel 4807 old Wall mounting device for Multipette plus Repeater plus Varipette 4981 203 009 022260596 with adhesive surface Battery 4980 215 003 022269119 3v 8 1 2 Combitips advanced Order No Order No Description Combitips advanced 0 1 mL 100 pieces 0030 089 812 0030089669 0030 089 405 0030089405 Eppendorf Quality 0030089510 Sterile individually wrapped 0030 089 618 0030089618 Biopur individually wrapped 0030 089 766 PCR clean Combitips advanced 0 2 mL 100 pieces 0030 089 413 0030089413 Eppendorf Quality 0030089529 Sterile individually wrapped 0030 089 626 0030089626 Biopur individually wrapped 0030 089 774 PCR clean Combitips advanced 0 5 mL 100 pieces 0030 089 421 0030089421 Eppendorf Quality 0030089537 Sterile individually wrapped 0030 089 634 0030089634 Biopur individually wrapped 0030 089 782 PCR clean Combitips advanced 1 0 mL 100 pieces 0030 089 43
5. uonn 11 Multipette plus Repeater plus Operating manual 6 Maintenance 2 6 1 Cleaning Any servicing activities above and beyond cleaning or changing the battery must be performed only by Eppen dorf AG or an Eppendorf AG service partner liquids Do not allow any liquids to penetrate the inside of the housing If liquid has penetrated the inside of the housing have the inner parts of the Multipette plus Repeater plus repaired only by service partners of Eppendorf AG Contact your responsible sales office before returning a product NOTICE Damage to device from penetration of E Clean any dirt off the Multipette plus Repeater plus as follows Moisten a cloth with soap solution and remove the dirt To disinfect the Multipette plus Repeater plus use isopropanol o 6 2 Change battery When the battery symbol appears in the digital display you can use the battery for approximately another two weeks 1 Push the lid in the direction of the arrow to open the battery compartment 2 Remove the old battery 3 Wipe over the new battery with a soft dry cloth 4 Insert the battery with the plus symbol and the labeling facing upwards 5 Close the battery compartment You can also use the Multipette plus the Repeater plus e without a battery see Empty Battery on p 10 6 3 Decontamination before shipment If you wish to return the pipette to Eppen
6. 200 uL 106 41 2 04 0 8 2 5 mL 50 uL 20 8 20 4 20 8 20 4 green 500 uL 30 5 32 2 0 3 1 5 5mL 100 uL 20 6 20 6 20 6 20 6 blue 1000 uL 05 50 0 25 25 10 mL 200 uL 30 5 21 0 20 6 1 2 orange 2 000 uL 30 5 10 30 25 5 0 25 mL 500 uL 20 4 32 0 20 6 23 0 red 5 000 uL 0 3 15 0 25 12 5 50 mL 1000 uL 30 3 23 0 20 5 25 0 light grey 10 000 uL 20 3 30 0 3 30 Kie oal darai is valid for the following test conditions in accordance with Liquid Water double distilled degased Test temperature 20 to 25 C constant to 0 5 C with original Eppendorf Combitips Number of determinations 10 before dispensing Combitip com pletely filled Operating temperature 4 C to 40 C Battery Lithium button cell 3V 210 mAh Service life about 2 years 8 Ordering Information j CAUTION Poor safety due to incorrect accessories and spare parts The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety func tion and precision of the device Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non recommended accessories and spare parts or from the improper use of such equipment Only use accessories and original spare parts recommended by Eppendorf Multipette plus Repeater plus Operating manual 8 1 Multipette plus Repeater plus Order No International Order No North America
7. mL 1 piece Eppendorf Quality 0030 089 723 0030089723 Adapter advanced 50 mL 1 piece Eppendorf Quality 0030 089 731 0030089731 Adapter advanced 25 mL 7 pieces Biopur individually wrapped 0030 089 740 0030089740 Adapter advanced 50 mL 7 pieces Biopur individually wrapped 8 1 4 Accessories Combitips advanced Fig 3 Combitips advanced Rack XJ A Combitips advanced Rack is avai advanced x 10 mL able for storing Combitips Order No International Order No North America Description 0030 059 506 Combilong Aspirating aid for removing liquids from volumetric flasks and tall bottles 2 pieces Multipette plus Repeater plus Operating manual Order No Order No Description International North America Combitube Aspirating aid for removing liquids from volumetric flasks and tall bottles 022261550 2 pieces Combitips advanced Rack 1 piece 0030 089 758 0030089758 Eppendorf Quality Eppendorf Biopur disposable articles are guaranteed to be sterile and free from pyrogen RNase DNA and ATP 9 Warranty 3 In the event of claims under warranty please contact your Eppendorf distributor Wear parts are excluded from the warranty When returning the Multipette plus Repeater plus please follow the instructions relating to decontamination see Decontamination before shipment on p 12
8. tube and push the filling lever right to the bottom The residual liquid is emptied Hold the Multipette plus the Repeater over a waste container 3 Press both ejection keys at the same time The Combitip in the case of 25 mL and 50 mL including adapter is released from the Multipette plus the Repeater plus The display goes out 4 On the Combitip 25 mL and 50 mL unscrew the reusable adapter from the Combitips The adapter is a wear part If significant wear is apparent do not reuse the adapter N Multipette plus Repeater plus Operating manual 4 6 Quick Start Only trained staff already familiar with the operating man ual and the Multipette plus the Repeater plus may work to these short instructions Follow the safety instructions Select Combitip With Combitip 25 50 mL connect adapter to the Combitip Insert Combitip see p 8 Fill Combitip a Immerse the tip of the Combitip in the liquid b Slowly draw up the filling lever 5 Reverse stroke a Pressthe dispensing lever b Discard the first dispensing step reverse stroke 6 Dispense a Place the tip of the Combitip against the wall of the tube b Pressthe dispensing lever 7 Discard the Combitip a Pushthe filling lever right to the bottom b Press both ejection keys at the same time B Ni 4 7 Empty Battery The Multipette plus Repeater plus can still function if the electronics fail or the battery is empty To set volume
9. 0 0030089430 Eppendorf Quality 0030089545 Sterile individually wrapped 0030 089 642 0030089642 Biopur individually wrapped 0030 089 790 PCR clean Combitips advanced 2 5 mL 100 pieces 0030 089 448 0030089448 Eppendorf Quality 0030089553 Sterile individually wrapped 0030 089 650 0030089650 Biopur individually wrapped 0030 089 804 PCR clean Combitips advanced 5 0 mL 100 pieces 0030 089 456 0030089456 Eppendorf Quality 0030089561 Sterile individually wrapped 0030 089 669 Biopur individually wrapped PCR clean 14 Multipette plus Repeater plus Operating manual Order No International Order No North America Description Combitips advanced 10 mL 100 pieces 0030 089 464 0030089464 Eppendorf Quality 0030089570 Sterile individually wrapped 0030 089 677 0030089677 Biopur individually wrapped 0030 089 820 PCR clean Combitips advanced 25 mL 100 pieces 0030 089 472 0030089472 Eppendorf Quality 0030089588 Sterile individually wrapped 0030 089 685 0030089685 Biopur individually wrapped 0030 089 839 PCR clean Combitips advanced 50 mL 100 pieces 0030 089 480 0030089480 Eppendorf Quality 0030089596 Sterile individually wrapped 0030 089 693 Biopur individually wrapped 0030 089 847 0030089693 PCR clean 8 1 3 Adapter advanced Order No International Order No North America Description 0030 089 715 0030089715 Adapter advanced 25
10. A 3 g 5 E 9 uonsod olu erp uonoejes euinjoA au 201 O U pexoo JOU Seu 1210 uonoe 35 do s ae ie Pap eos p JOU exojs esie ed s 2 S 184 ou p1eosip sAemje diiquio eui Guy sayy enc 598358 E s Bumad d 8 3 3 3 a g ene Duisuedsip ou jo ured gus au esn poinaoxo usoq JOU SEY SORS DUNC 2 56 8 E i pinbi pesseBep est 856 i s Bir s qqnq ute surejuoo 00001 3 E N ate bu eu En _ d en siduiquog senjeA uoisi gt e1d Koein gt 2e 1004 9 5 E c 5 3 payesidse eq jouueo pinbi og 8515 5 Bunjee si dyqwog tezis ui seseejoui qqnq aiv 25088 diiquio eui eoejded r Qni 92 ni Tel b soto SE slonuoo Bunejedo fei i 4 G p g 8 8 2 sjo uoo Bunejedo anja Laer enednjniv Meu esp 086 anad nyy pio ul diquio2 Tw Sz by 6 5 co wedau 10 snid sayeaday snjd syad nyy eui ui pues E 2 u 5 8 288 25 ale 2 are soluono g3 7 5 oe 9 2 E au 1nouii ejqissod ale uonoejes euinjo pue pei S Ano J p T SSSI TS lqISIA s ojo 2 3852 6 BS nena Renee Rep Aqoeuoout pej e sui ueeq sey Aleneg 2 lt oz o o an sod eui yey euns Bu yeu Anoe neq y ie OD eee 3 2 OW G sie tto zn ieyeq mau e 95 HM Kfeldsip yuelg dquioO ou vejd y pouesur ao N ji Asayeq eu e ejdeu umop Gu o SOOM OM 1Xeu au uiuym ueneq ay EE TES TE o kur
11. aintenance 12 6 1 GIEANINd c 12 6 2 Change battery a aa sa sra em ka a RR RR ed 12 6 3 Decontamination before shipment 12 7 Technical data 13 8 Ordering Information 13 9 Waranty sisse cc ee 16 10 Disposal dua yk gasas wu 16 10 1 Disposal lt sl ka k vu aee art a ee cr Rp qd RARE 16 1 User instructions 1 1 Using this manual Please read this operating manual completely before using the device for the first time gt Please view this operating manual as part of the product and keep it somewhere easily accessible When passing the device on to third parties be sure to include this operating manual 2 Product description D 2 4 Features In combination with Eppendorf Combitips advanced the Multipette plus the Repeater plus forms a dispensing system for repeat dispensing operations Multipette plus Repeater plus Operating manual Fig 1 Multipette plus Repeater plus 1 Volume selection dial 2 Position indicator 3 Battery compartment 4 Digital display 5 Dispensing lever 6 Filling lever 7 Ejection keys 8 Serial number 2 2 Combitips advanced A supply of liquid is aspirated into the Combitip advanced and then dispensed step by step The built in electronics automatically recognize the Combitip advanced insert
12. dorf AG or an Eppendorf AG service partner to be checked or repaired please note the following CAUTION Use of a contaminated device may result in personal injuries and damage to the device Clean and decontaminate the Multipette plus Repeater plus before shipping or storage according to the cleaning instructions Hazardous substances are solutions presenting a hazard to health potentially infectious agents organic solvents and reagents radioactive substances proteins presenting a hazard to health DNA 1 Please note the information in the document Decontamination certificate for product returns You can find it as a PDF file on our homepage www eppendorf com 2 Enter the serial number of the Multipette plus Repeater plus in the decontamination certificate 3 Enclose the fully completed decontamination certificate for returned goods with the Multipette plus Repeater plus 12 Multipette plus Repeater plus Operating manual 7 Technical data Combitip Testing Error limits advanced volume Error Systematic Random error error 96 HL uL 0 1 mL 2 uL 1 6 0 032 23 0 30 06 white 20 uL 10 02 20 20 4 0 2 mL 4 uL 21 3 0 052 22 0 20 08 light blue 40 uL 0 8 20 32 21 5 20 6 0 5 mL 10 uL 20 9 20 09 21 5 20 15 violet 100 uL 08 08 06 20 6 1 20 uL 20 9 20 18 20 9 20 18 yellow
13. ed and the position of the volume selection dial and the current dispensing volume appears in the display Nine Combitips advanced with a variety of filling volumes are available for single use see Choose Combitip on p 7 The Combitips advanced 25 mL and 50 mL can only be used in combination with an adapter The adapter is a wear part For each Combitip advanced you can use the volume selection dial to set 20 different volumes The smallest volume can be dispensed up to a max of 100 times and the largest volume a max of 5 times ll 4 1 3 5 6 7 Fig 2 Combitip advanced 1 Dispensing volume with the 2 Graduation manual dispenser at selection dial position 1 3 US patent numbers 4 Maximum volume 5 Color code 6 Coding 7 Piston 2 3 Combitips plus Multipette plus Repeater plus can also be used with Combitips plus predecessor model Multipette plus Repeater plus Operating manual 3 Safety 3 1 Intended use The Multipette plus Repeater plus is a lab device and in combination with a Combitip plus Combitip advanced it is intended for dispensing aqueous solutions in the volume range between 1 uL and 10 00 mL In vivo applications applications in or on the human body are not permitted The Multipette plus Repeater plus may only be operated by trained specialist staff All users must have read the operating manual carefully and familiarized
14. r plus by the housing NOTICE Damage to device from penetration of liquids Do not allow any liquids to penetrate the inside of the housing If liquid has penetrated the inside of the housing have the inner parts of the Multipette plus Repeater plus repaired only by service partners of Eppendorf AG Contact your responsible sales office before returning a product Multipette plus Repeater plus Operating manual NOTICE Material damage from incorrect use Only use the product for its intended purpose as described in the operating manual Ensure adequate material resistance when using chemical substances Do not use the Multipette plus Repeater plus with liquids which attack the materials ABS ASA PBT PC POM and PPS Do not use the Combitip with liquids which attack the materials PP PE or LCP In case of doubt contact your local Eppendorf partner T 5 i E D s En a e plus Repeate Multipette plus 4 Operation Choose Combitip Use the volume table to select volumes and Compitip plus 4 1 ejdepe 03110005 eui yum pesn eq 1snui sdiiquio qui 0 pue TW Gz 1 1096 rig rios rise 11006 Nosy rog Woe 19 mg 06 TWS 8 qu0 8 us WO 92 qug 11009 11092 Troop 11192 mog most og 1192 Tes moe 02 TwG 9 quse qwgz e quy qug 11002 11099 mose msze mor moe 1102 gg mez m z mo
15. rds Or insert the Combitip and then push the filling lever downward 4 3 Select volume gt Turn the volume selection wheel until it locks into the desired position The volume selection wheel has 20 positions They are indicated with the figures 1 to 10 Positions between these numbers and position 0 5 are indicated with a spot When setting small volumes position 5 and below the operating path of the dosage lever is reduced automati cally The dosage lever changes its position when the vol ume selection wheel is turned You cannot turn the volume selection wheel beyond position 10 Do not turn the volume selection wheel beyond 10 or 0 5 Turn the volume wheel back to its initial position 4 3 1 Filling Combitip and reverse stroke 1 Immerse the tip of the Combitip in the liquid 2 Slowly and uniformly push the filling lever up to the stop The display is blinking the Multipette plus Repeater plus is not yet ready for operation Multipette plus Repeater plus Operating manual 4 3 2 Discard first dispensing step reverse stroke 1 Wipe the Combitip tip using lint free cell tissue 2 Place the Combitip tip on the tube wall 3 Actuate the dispensing lever once and discard the liquid reverse stroke This step which brings the system to the basic position should be completed independent of the volume setting The display is no longer blinking and displays the dispensing volume The Multipet
16. s use the volume table see p 7 or proceed as follows in order to determine the position required on the volume selection wheel 1 Read the volume for position 1 on the volume wheel from the Combitip fig 2 1 2 Divide the desired volume by the volume with position 1 Examples for Combitip Volume for position 1 100 uL Desired volume of dosage 200 uL Calculation 200 100 2 Position to be set 2 Desired volume of dosage 750 uL Calculation 750 100 7 5 Position to be set between 7 and 8 Desired volume of dosage 50 uL Calculation 50 100 2 0 5 Position to be set before 1 10 UJ09 JJ0pUedde MMM JNO uo 4S3 au 104 enpeoojg d 01510 d sayeaday au jo snid syad nyy eui jo sseujoe4100 pue uols E 2 S p gpus S dOS ue pulj ued noA s o e Buisu dsip Jusna d o AurejnBeJ payoayo eq jsnwi sn d Jay Hay s 92 e depe meu sr u Pev 52 amp ues eAnoejep s Josues c o 3 E wedau 10 snjd sayeaday snjd syad y nyy eui u p S Key uono l I j A 3 S E im Anoeuoo diliquuoo um uesu ou u 40900 peuorisod jou s dyiquiog ut 8 8 Su Bulyonoy nouis AN ueajo Jou SI ease Bununoui TO dad au jo wiy 5 3 5 o a d j qu oo mau e asn JO yJojo Js ow UNIA edi diiquio eui 10 joueq diliquuoO eui j ES uon 3 252 T zs sfe dsiq uonoejes euinjo
17. sg mszy Woz rig moss 1192 Wort riss 99 quos quz quz wot 111006 11009 most mosz mezz 11001 Tos oz mo wor Tuse WOE Jusi uo Tus WO 190 7w OS quo z Tusz qus Iwo Moss Moos mosz Tu sz 11008 11002 Moog moos Toor moose mooz 11001 7w 04 101007 mose loos mosz mooz Trost 101001 1109 Tug mooz 10192 ost 1192 moot 10192 mos mez qw ez mog 1102 mog mos mory moe moz 1101 wor Tov mse wos mez moz ms Trot 119 7u so m r my me or me m mr mz qar go OL qe m ms Ty me mz m Turpo BUILUN OA uep yw diquio2 a pl 9 02 Sz 99 OS 001 sde s Buisuedsip jo 1equinu wnwixey S Sz 2 gi L 90 peip uonoejes euinjoA 8U jo 01504 sn d eyedninjy eui yum sdiiduio Buisn 104 ajqe euinjoA See the safety instructions and the general illustration see Fig on p 4 e Multipette plus Repeater plus Operating manual 4 2 Insert Combitip NOTICE Damage to property due to incorrect insertion of the Combitip For Multipette plus Repeater plus manufactured before 12 15 2009 Do not press the ejection keys when a Combitip is inserted 1 If using 25 mL or 50 mL Combitips connect the adapter to the Combitip outside the Multipette plus Repeater plus 2 Slide the filling lever right to the bottom and hold it there 3 Push the Combitip from below into the Multipette plus Repeater plus and in doing so hold the filling lever slid downwa
18. te plus Repeater is ready for operation the full range of dispensing steps are available Comments f you do not fully fill the Combitip the display will not blink Press the dispensing lever fully downward as often as necessary until liquid escapes from the Combitip plus Discard the liquid Then you can dispense the set volume Between dispensing steps you can change the volume using the volume selection dial Pressing the filling lever too quickly may create a too high vacuum The liquid is enriched with air bubbles which could cause dispensing inaccuracies With a volume selection dial setting larger 5 the aspiration of a large air bubble can lead to drip formation after each dispensing step particularly with 10 25 and 50 mL Combitips A small air bubble in the upper cylinder chamber has no effect on dispensing The Combilong Combitube is an aspiration aid for the Multipette plus Repeater plus The Combilong Combitube enables direct liquid aspiration from bottles 4 4 Dispense 1 Place the tip of the Combitip against the wall of the tube 2 Push the dispensing lever up to the stop If the remaining liquid is not enough for complete dispens e ing of the set volume for the last dispensing step the resid ual stroke lock automatically prevents an insufficient amount from being dispensed After the Combitip has been refilled discard the first dispensing step 4 5 Release Combitip 1 Hold the Combitip in a
19. themselves with the device s mode of operation CAUTION Danger to persons from careless use Never point the opening of a Multipette plus Repeater plus equipped with a Combitip at yourself or anyone else Only perform dispensing operations when you do not endanger yourself or anyone else CAUTION Poor safety due to incorrect accessories and spare parts The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety func tion and precision of the device Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non recommended accessories and spare parts or from the improper use of such equipment Only use accessories and original spare parts recommended by Eppendorf NOTICE Damage to device from opening the housing Do not remove the housing of the Multipette plus Repeater plus Do not allow the housing to be opened by anyone except service staff authorized by Eppendorf NOTICE Damage to sensor from damaged or incorrectly inserted Adapter plus When using 25 mL and 50 mL Combitips make sure that the adapter is in a perfect condition The adapter is a wear part Do not use the Adapter if it is damaged or visibly worn Connect the Adapter and the Combitip always outside of the Multipette plus Repeater plus Do not turn the inserted Combitip Always hold the Multipette plus Repeate
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