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1. Graph Display The Y axis indicates the absorbance value and the X axis is for the wavelength The maximum absorbance value is automatically 29 Quawell User s Manual adjusted to the highest absorbance value The user is also allowed to manually enter the absorbance value Sample ID there are two windows that display the sample ID information The left window displays the sample ID input by the user The default is My Sample The right window displays the ID number for that sample ID The ID number begins at 1 and increases by 1 for each measurement Changing the Sample ID will automatically reset the ID number to 1 600 nm Abs 1mm absorbance at 600nm e Baseline if selected the absorbance value of the baseline is subtracted from the absorbance at 600nm Selected nm user select wavelength Abs 1mm absorbance at selected nm Green vertical line indicates the peak position of the wavelength for selected nm 30 Quawell Users Manual 13 THE PREDEFINED FLUORESCENT DYES LIST The list works with both the Microarray and Labeled Proteins modules This list contains a pre defined list of fluorescent dyes as shown below 5 Dye Chromophore Editor p Dye Chromophore List 260 nm Cy3 150000 250000 Alexa Fluor 488 71000 Alexa Fluor 546 104000 Alexa Fluor 555 150000 Alexa Fluor 594 73000 NONE NONE Dye Editor2. 17 CONTACT INFORMATION Quawell Technology Inc P O Box 612738 San Jose CA 95161 Tel 408 329 6241 www quawell com E mail info quawell com 41
3. Cy3 5 T M cm 50000 nm 581 260 nm 0 08 280 nm 0 24 Update 31 Quawell Users Manual 14 LIGHT INTEGRATION This module is used for adjusting the light integration The integration number is set to normal before shipping The light integration and detector systems are functioning normally if the red and black spectra appear as in the image below However if the image appears as below with the light integration spectrum too high or too low the light integration can be adjusted with this feature prm Rass 99 aee ume lt E r Seve Enter new light intergration value 1 12 2 e a Nace Acd wis e Original Value 1 000 1000 32 Quawell Users Manual Adjusting the light integration 1 Select the module from the horizontal tab and the path from the vertical tab 2 The original integration number appears in the window Original Value Enter the number in the window Enter new light integration value 1 12 4 Click the Check and the black spectrum will display in the window 5 Increase the integration value from 1 up to12 stopping when the black and red spectra are close Nucleic Acid Protein 4280 Cell Cultures DV NISI Protein amp Label Micro Array Original Value 1 M 200 250 30
4. and lower surfaces with soft dry paper Quawell Users Manual 4 Export the data to an Excel worksheet by clicking the Report button WaveLength 1 Absorbance 1 Wavelength 2 Absorbance 2 1 My Sample 350 0 964 600 2 My Sample 350 0 985 600 3 My Sample 350 1 165 600 7 8 2010 6 50 48 AM Quawell User s Manual 2 THE PATENT PENDING TECHNOLOGIES Figure 1 Initially the sample is loaded and the upper arm is closed Figure 2 The upper arm is lifted by the actuator due to molecular effects the sample is broken into two separate parts one part is attached to lower surface and the other part is attached to upper surface Figure 3 After reaching its home position the upper arm is lowered A liquid column is formed when the upper arm is lowered to the position where the two droplets touch The measurement is then performed at this position without moving the upper arm upwardly again Quawell User s Manual 3 INSTALLATION Computer Requirements Microsoft Windows XP Vista or later Microsoft Office 2003 or later CPU 400MHZ or higher e 64MB RAM USB port e Monitor 1024x768 Software Setup Note Do not connect Q5000 to PC before installing the software e Insert the CD into CD driver and double click the file setup in the folder CD RAM Q5000 V1 0 0 Follow the prompts to finish installation of the software e Connect Q5000 to PC with the USB cable and the driv
5. position of the wavelength for Dye 2 Measurement Range Concentration Reproducibility 0 1 20 mg ml BSA t 296 0 2 100 uM Cy3 2 26 Quawell Users Manual 11 UV VIS MEASUREMENT Overview The Q5000 can function as a general use laboratory spectrophotometer The UV Vis measurement module provides the operator with a sample absorbance range from 220 to 850 nm UV Vis Measurement Screen Features Select UV Vis from Q5000 main menu and the module interface will appear on the screen Coe Oo We m j 7 7 2010 12 08 51 AM Abs 1mm 1 1 0 E Sample ID Sample 0 1 350 T Abs1 1mm 0 2 600 Abs2 1mm 0 Baseline Multi Spectra Blank Clear Data Save Graph Report Sample ID iD WaveLenath 1 Absorbance 1 WaveLength 2 Absorbance 2 27 Quawell User s Manual Graph Display The Y axis indicates the absorbance value and the X axis is for the wavelength The maximum absorbance value is automatically adjusted to the highest absorbance value The user is also allowed to manually enter the absorbance value Sample ID there are two windows that display the sample ID information The left window displays the sample ID input by the user The default is My Sample The right window displays the ID number for that sample ID The ID number begins at 1 and increases
6. 0 350 400 450 500 550 600 650 700 750 If you have questions about the light integration please contact your local distributer or Quawell at info quawell com 33 Quawell Users Manual 15 POTENTIAL SOURCES OF ERROR Sample Overlap Sample residue on either measurement surface will affect the result We suggest that the user Clean the two surfaces with dry soft lab paper after each sample measurement Clean the surfaces with 2 ul de ionized after measuring highly concentrated samples Thoroughly clean the surfaces and surrounding area with de ionized water after completing all measurements Before sample measurement blank the machine as described 1 Open the appropriate application module 2 Lift the upper arm and add 2 5 ul of blanking reagent to the lower surface 3 Lower the upper arm and click the Blank button 4 Lift the upper arm and clean the blanking reagent from both surfaces with de ionized water 5 Add 2 5 ul blanking reagent to the lower surface 6 Lower the upper arm and click the Measure button 7 Ifthe value in the Abs260 window is higher than 0 004 repeat these blanking steps Sample Homogeneity Non homogenous samples would cause significant deviation in the data generated by any measurement system including the spectrophotometer 34 Quawell User s Manual Effect of Evaporation Sample evaporation could cause a 1 296 deviation in the sample s co
7. 23 Quawell Users Manual 10 LABELED PROTEINS Overview The Labeled Proteins function will simultaneously measure both protein and fluorescent dye concentrations at appropriate wavelengths Proteins and Labels Screen Features Select Proteins and Labels from the Q5000 main menu and the module interface will appear on the screen im 00 i s s lt a Help n 7 7 2010 12 09 25 AM Abs 10mm 80 800 zd Sample ID Sample SampleType EC Abs 1mg n v SW 260 nm Abs 1mm o 280 nmAbs y 260 280 p mg ml p 340 Baseline Dyel nm 550 i uM p Abs 0 Dye2 NONE nm o uM 0 Abs 0 750 Baseline 300 450 50 50 600 60 700 750 Multi Spectra Blank Clear Data Save Graph Report Measure Sample iD 104 Sample Type EC 2804 260 280 Con Dyet Dyet A Dyel Con Dye2 Dye2 4 Dye2 Con Lis 24 Quawell User s Manual e 340 Baseline the absorbance value of 340 nm baseline is only used for the sample concentration calculation reported at 280 nm The default of 340 nm baseline is checked e 750 Baseline it normalizes the visual spectrum display and calculates a baseline between 400 nm and 750 nm for dye concentration Graph Display The Y axis indicates the absorbance value and the X axis is for the wavelength The maximum absorbance value is automatically adjusted to the highest absorbance va
8. 350 0 985 600 0 006 3 My Sample 3 350 1 165 600 0 004 7 8 2010 6 50 48 AM Report Date 14 Quawell Users Manual 7 NUCLEIC ACIDS Overview The Q5000 measures nucleic acid absorbance automatically calculates concentration and displays the data on the screen Nucleic Acid Screen Features Select Nucleic Acid from the Q5000 main menu and the user interface shown below will appear on the screen l Nucleic Acid s e s a r 7 7 2010 12 06 43 AM Abs 10mm 10 H 10 0 Sample ID Sample 0 80 4 SampleType EC dsDNA 50 80 Sw 230 nm Abs 10mm p 70 260nm Abs 10mm 0 6 0 280nm Abs 10mm 0 260 280 0 5 0 260 230 g 40 ng ul p 30 340 Baseline 20 1 0 nm 220 230 240 250 260 270 28 290 300 310 320 330 340 350 360 No ID SampleType sw Swabs 260Abs 280Abs 260 280 250 230 Concentration 4 Blank Clear Data Exit Multi Spectra L e 340 nm Baseline the absorbance of nucleic acid at 340nm should be very 15 Quawell User s Manual nearly zero If the Baseline option is checked the absorbance value of the baseline is subtracted from the absorbance at 260 nm and all spectra are referenced to this zero The default value is check Graph Display The Y axis indicates the absorbance value and the X axis is for the wavelength The maximum a
9. 5 mm x 210 mm Weight 2kg Operating Voltage 12 vdc Power Consumption 15 W operating Power Consumption 1 5 W standby surface Construction 303 stainless steel and quartz fiber Software Compatibility Windows XP and Vista 32 bit CE Approval Units sold in Europe 38 Quawell User s Manual B Absorbance Calculation When the Q5000 Spectrophotometer is blanked a spectrum is taken of a reference material blank and stored in memory as an array of light intensities by wavelength When a measurement of a sample is taken the intensity of light that was transmitted through the sample is recorded The sample intensities along with the blank intensities are used to calculate the sample absorbance according to the following equation Abs Lus Thus the measured light intensity of both the sample and of the blank are required to calculate the absorbance at a given wavelength 39 Quawell User s Manual C Concentration Calculation BEER S LAW The Beer Lambert equation is used to correlate the calculated absorbance with concentration A E b c Where A is the absorbance represented in absorbance units A E is the wavelength dependent molar absorptive coefficient or extinction coefficient with units of liter mol cm b is the path length in cm and c is the analyze concentration in moles liter or molarities M 40 Quawell User s Manual
10. 8 Quawell User s Manual Microarray Screen Features 18 Measurement Range ueri Et A 20 9 Protein A280 sU Dead wiV AREE 21 OVESIVIGW cocotte ce creo 21 Protein 280A screen features 21 Measurement Range 23 10 Labeled Proteins eoi Yee Loc adidas RR LER Er UHR ud 24 erar ccc PPP 24 Proteins and Labels screen features 24 Measurement 26 11 UV Vis Cl coke aid ev 27 TP TM 27 UV Vis Measurement screen features 27 12 Gell C ltureS T 29 COVOLVIGUW 29 Cell Culture Module Screen Features 29 13 The Predefined Fluorescent Dyes List 31 EGIlOr URB T 31 14 Light Integration 32 Adjust the dghtintegratori sce ia ge cnm dn oe etae eee nde 33 15 Potential Sources of Error 34 odimpl
11. Dye1 or Dye2 3 pmol nl concentration of Dye1 or Dye2 in pmol nl 4 Vertical Lines the green line indicates the peak position of the wavelength for Dye 1 and the blue vertical line indicates the peak position of the wavelength for Dye 2 Measurement Range Cy3 Concentration pmol nl Reproducibility 0 2 100 0 2 20 Quawell Users Manual 9 PROTEIN A280 Overview Protein A280 measures the protein s absorbance at 280 nm and calculates the concentration Since no protein standards are required Protein A280 is fast and convenient Protein A280 Screen Features Select Protein A280 from the Q5000 main menu and the module interface will appear on the screen adis lt Help Gr 7 7 2010 12 08 46 AM Abs 10mm 10 H 100 Sample ID My Sample 0 90 SampleType Abs 1mg n v 8 0 SW 1280 10mm 0 70 260nm Abs 10mm fo 6 0 280nm Abs 10mm n 260 2 280 5 0 mg ml g 340 Baseline Multi Spectra Blank Clear Data Save Graph Report Measure SamplelD 10 8 SampeType SW Abs 260 Abs 280 Abs 260 280 Concentration 4 21 Quawell User s Manual 340 nm Baseline the absorbance value of 340 nm baseline is only used for the sample concentration calculation reported at 280 nm The default of 340 nm baseline is checked Graph Display The Y axis indicates the ab
12. Quawell User s Manual Quawell Q5000 UV Vis SPECTROPHOTOMETER USER S MANUAL V1 0 1 Rev 07 01 2010 Quawell CONTENTS User s Manual 1 5 What IS The 8000 Aca a a uha 5 How Operate ores relire 7 2 The Patent Pending Technologies 8 3 Installati n m I 9 Computer Requirements esses 9 Software SOM Cat Lat E RN oe 9 4 Main Men 10 The Measurement it e 10 Bic MOONS rrr 10 5 Application module 13 aries EE 13 Current Data Display WIDQOOWS iioii hene eer p e beca 13 Main Function cuts diu do Moi c et teeth oho tete dt 13 Cio TTE 13 Data Tabl Ei MN p 13 6 Saving the Data and information 14 7 Nucleic AGUAS uei adecuadas a ma 15 M dA dE D QM E ME E 15 Nucleic Acid Screen Features 15 Measurement Range EHE REI MEER ius 17 Sample 17 MEM Dperrjd 18 ONY ORV OW 1
13. The user can select the option based on the particular need Current data display windows display the data and information of the sample being measured Main function Panel contains all main functions for sample measuring and data handling Graph area displays the absorbance Y axis and spectrum X axis Data Table used for holding and displaying the accumulated data The maximum number of rows is 1000 13 Quawell Users Manual 6 SAVING THE DATA AND INFORMATION Note Q5000 program does not save the data automatically All data and information for the sample being measured will be lost if it is not saved User can save the data using the Report function to export the data from the data table to an Excel spreadsheet ES BN Home Insert Pagelayout Formulas Data Review View IR X Ju gt ge Conditional Formatting 3 Imet x n da 1 e gt as Table Delete 8 27 d ici F m z u g o A EE ij Cell Styles Bi Format 2 Fiter Seed Clipboard Font mj Alignment Number J Styles 1 Cells Editing Al Gas sample ID 10 WaveLength 1 Absorbance 1 WaveLength 2 Absorbance 2 1 My Sample 1 350 0 964 600 0 004 2 My Sample 2
14. bsorbance value is automatically adjusted to the highest absorbance value The user is also allowed to manually enter the absorbance value Sample ID there are two windows that display the sample ID information The left window displays the sample ID input by the user The default is Sample The right window displays the ID number for that sample ID The ID number begins at 1 and increases by 1 for each measurement Changing the Sample ID will automatically reset the ID number to 1 Sample Type EC chose the sample type for the sample to be measured There are 3 different sample types for nucleic acids dsDNA ssDNA and The default is dsDNA The sample type is disabled after blanking The extinction coefficient of the sample selected will appear in the EC window Sam E Coe 50 e SW and Abs 10mm user selected wavelength and corresponding absorbance The selected wavelength and absorbance are not utilized in any calculations 260 nm Abs 10mm absorbance of the sample at 260 nm 280 nm Abs 10mm absorbance of the sample at 280 nm 16 Quawell User s Manual e 260 280 ratio of absorbance at 260 nm and 280 nm 260 230 ratio of absorbance at 260 nm and 230 nm ng ul sample concentration in ng ul Measurement Range dsDNA Concentration Reproducibility Abs accuracy 1 mm 1 to 5000 ng ul 1 100 2ul 2 gt 100 ng ul 2 Sample Volume The
15. by 1 for each measurement Changing the Sample ID will automatically reset the ID number to 1 4 1 nm select the wavelength by using the up down arrows Abs 1 1mm corresponding absorbance at 4 1 nm 4 2 nm select the wavelength by using the up down arrows Abs 2 1mm corresponding absorbance at 4 2 nm Baseline if selected the absorbance value of the baseline is subtracted from the absorbance of Abs1 and Abs2 Vertical Lines the green line on the absorbance wavelength graph indicates the peak position of the wavelength for 4 1 nm and the blue vertical line indicates the peak position of the wavelength for A2 nm 28 Quawell Users Manual 12 CELL CULTURES Overview The Q5000 allows laboratories to monitor the density of suspended cell and microbial cultures by measuring their light scatter at 600 nm Cell Cultures Screen Features Select Cell Cultures from Q5000 main menu and the module interface will appear on the screen 5 OON aal nene E Jr 72722010 12 10 12 AM Abs 1mm n 1 0 es Sample ID My Sample 0 600nm Abs 1mm 0 Baseline Textl Selected 1 350 Selected Abs 1mm 0 nm 200 20 300 350 400 450 500 550 600 650 700 750 80 850 Exit Multi Spectra Blank Clear Data Save Graph Report Measure Sample ID ID 600nm Abs 1mm Baseline nm Selected nm Selected Abs 1mm L
16. e OVelap aside ne nro SEM Drop tret ettet 34 Sample 34 Effect of evaporation r 35 Insufficient sample volume 35 16 Troubleshooting U 36 Quawell User s Manual USB Connection Er dro osse 36 Sample Accuracy and Reproducibility 36 17 eer 37 18 5 38 A Instrument specifications 38 B Absorbance calculation a ioc so tris Eo tad 39 C Concentration Calculation Beer s Law 40 D Contact Information rep rre retener oae aite inito deis 41 Quawell Users Manual 1 OVERVIEW What Is The Q5000 Quawell Q5000 is a micro volume UV Vis spectrophotometer Its full spectrum 200 850nm allows Q5000 to measure nucleic acids microarray probes purified proteins and fluorescent and fluorescently dyed proteins monitor the density of suspended cellular bacterial and yeast cultures and perform general laboratory UV Vis absorbance measurements A unique technology used in the Q5000 holds 0 5 2 5 ul samples between upper and lower measurement surfaces without the u
17. er will be installed automatically Version Conflict If you install Q5000 software in a computer with Windows Vista please click option Yes when Version Conflict message appear as below A file being copied is older than the file currently on your system It is recommended that you keep your existing file Do you want to keep this file Quawell Users Manual Abort Retry and Ignore option When you see a message that requires you select from options Abort Retry or Ignore please click option Ignore and continue to install Q5000 software 10 Quawell 4 MAIN MENU Users Manual Q5000 main menu contains two tabs the Measurement tab and the Tools tab The default is Measurement tab The Exit buttons in both tabs will exit the Q5000 program l Q5000 V3 0 20 l Q5000 V3 0 20 Quawell Q5000 UV Vis Spectrophotometer Quawell Q5000 UV Vis Spectrophotometer Nucleic Acid Protein A280 Microarray Labeled Proteins UV Vis Measure Cell Culture The Measurement tab 1 n ame UIS Nucleic Acids Microarray UV Vis Measure Protein A280 Labeled Protein Cell Culture 11 Measurement Light Intergraion Dye List ELE www quawell com Quawell User s Manual The Tools tab 1 Light Integration Allows the user to adjust the light integration 2 Dye List edit the dye data Software Version Numbe
18. isplay and calculates a baseline between 400 nm and 750 nm for dye concentration Graph Display The Y axis indicates the absorbance value and the X axis is for the wavelength The maximum absorbance value is automatically adjusted to the highest absorbance value The user is also allowed to manually enter the absorbance value Sample ID there are two windows that display the sample ID information The left window displays the sample ID input by the user The default is Sample The right window displays the ID number for that sample ID The ID number begins at 1 and increases by 1 for each measurement Changing the Sample ID will automatically reset the ID number to 1 Sample Type EC chose the sample type for the sample to be measured There are 3 different sample types for nucleic acids dsDNA ssDNA and The default is dsDNA The sample type is disabled after blanking The extinction coefficient of the sample selected will appear in the EC window e SW and Abs 10mm user selected wavelength and corresponding absorbance that are not utilized in any calculations 260 280 ratio of absorbance at 260 nm and 280 nm ng ul sample concentration in ng ul e Dye and Dye 2 Selection Windows 1 Dye1 or Dye2 drop down list displays the dye that is pre predefined 19 Quawell Users Manual using the Dye List Editor Please see Section 11 for details on how to predefine the list 2 Abs absorbance of
19. lue The user is also allowed to manually enter the absorbance value Sample ID there are two windows that display the sample ID information The left window displays the sample ID input by the user The default is My Sample The right window displays the ID number for that sample ID The ID number begins at 1 and increases by 1 for each measurement Changing the Sample ID will automatically reset the ID number to 1 Sample Type chose the sample type for the sample to be measured 1 1Abs 1mg ml 2 BSA 3 IgG 4 Lysozyme e SW and Abs 10mm the user selected wavelength and corresponding absorbance The wavelength can be selected by using the up down arrows or input the number in the wavelength box The user selected wavelength and absorbance are not utilized in any calculations Abs280 10mm absorbance of the sample at 280 nm 260 280 ratio of the absorbance at 260 nm and 280 25 Quawell e mg ml sample concentration in mg ml Dyel1 and Dye 2 Selection Windows 1 Dye1 or Dye2 drop down list displays the dye that is User s Manual pre predefined using the Dye List Editor See Section 11 for details on how to predefine the list 2 Abs absorbance of Dye1 or Dye2 3 uM concentration of Dye1 or Dye2 in uM 4 Vertical Lines the green line on the absorbance wavelength graph indicates the peak position of the wavelength for Dye 1 and the blue vertical line indicates the peak
20. ncentration Insufficient Sample Volume We recommend 2 5 ul for normal sample measurements 35 Quawell User s Manual 16 TROUBLESHOOTING USB Connection Error This error screen usually indicates that the Q5000 and PC connection failed To correct the error unplug the USB cable wait for 2 3 seconds and plug it again Sample Accuracy and Reproducibility If the sample data are inaccurate and or not reproducible please refer to Section 18 POTENTIAL SOURCES OF ERROR 36 Quawell User s Manual 17 MAINTENANCE The primary maintenance requirement of the Q5000 spectrophotometer is to keep the measurement surfaces clean Upon completion of a measurement wipe the sample from the upper and lower surfaces Clean the surfaces and surrounding area with de ionized water to prevent sample carryover and residue buildup 37 Quawell 18 APPENDICES User s Manual A Instrument Specifications Minimum Sample Size 0 5ul Optic Path Length 0 2 1mm auto selected Light Source Xenon flash lamp Wavelength Range 200 850 nm Wavelength Resolution inm Wavelength Accuracy 1 nm Absorbance Range 0 01 100 Absorbance Precision 0 002 Abs 1mm Absorbance Accuracy 196 Detector Type 3648 element linear silicon CCD array Detection Limit 1ng ul dsDNA Max Concentration 5 000 ng ul dsDNA Measurement Cycle lt 8 seconds Dimensions 14
21. r it is on the top right side of the menu Please check our website www quawell com on the bottom of the main menu for software upgrades and product news 12 Quawell 5 APPLICATION MODULE INTERFACE User s Manual The following is the Q5000 sample measurement user interface The window is divided into five different areas Nucleic Acid E 5 25 2010 10 14 18 AM Abs 10mm ps 750 10 Sample 8 a Checkbox panel SampleType EC 50 650 60 0 oeo 10mm 42754 55 0 Current data display windows Zeon AEA Pang 42754 0 280nm Abs 10mm 27 43 40 0 260 280 1 558 350 Graph area 260 230 Fj 30 0 ng ul 2137 699 250 Fin Main function panel V 340 Baseline 15 0 10 0 nm 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 Exit Multi Spectra Blank Clear Data Save Graph Report oo Measure No 5 ID amp Sample Type Wave Length 230 250 280 250 280 260 230 Concentration 8 Sample 8 dsDNA 50 42 754 42 754 7 43 1 558 1 2137 699 7 Sample 7 dsDNA 50 b7 01 1 458 2 241 4157 8 m Sample 6 dsDNA 50 Data table 006 4333 0 565 1 289 5 My Sample 5 dsDNA 50 0 013 0 077 0 077 0 05 4 Mu Samne 4 dsDNA 230 Anm AMA AMA naa 1 499 LT Checkbox Panel contains online help and other options
22. re is no specific requirement for the sample volume however for the best accuracy and reproducibility we recommend 2 2 5ul 17 Quawell Users Manual 8 MICROARRAY Overview The Microarray module analyzes fluorescently labeled nucleic acid probes It simultaneously measures the concentration of the fluorescent tag and the nucleic acid at appropriate wavelengths Microarray Screen Features Select Microarray from the Q5000 main menu and the application interface will appear on the screen UNUS TOP neem ciis lt a LI Help Or 7 7 2010 12 08 05 AM Abs 10mm 80 800 ES Sample ID My Sample 0 get SampleType dsDNA gt 50 67 5 sw e p 260 280 j ng 7 ul 340 Baseline Dyel m 550 pmol ul 0 Abs 0 Dye2 NONE gt m o pmol ul 0 Abs 0 nm 220 250 4 750 I Exit Multi Spectra Blank Clear Data Save Graph Report Measwo Sample iD ID t Sample Type Sw Abs 260 280 Con Dyel Dvet Dyet Con Dye2 Dye2A Dye2Con 4 L 18 Quawell User s Manual 340 nm Baseline the absorbance value of the 340 nm baseline is only used for the sample concentration calculation reported at 260 nm The default of 340 nm baseline is checked e 750 nm Baseline it normalizes the visual spectrum d
23. se of a cuvette and measures the sample in about 5 seconds with a high degree of accuracy and reproducibility How to Operate Operation of the Q5000 is simple and efficient The Q5000 automatically performs all necessary measurements and calculations The data are accumulated in the module data window and are easily exported to an Excel spreadsheet 1 Lift the upper arm add 2 2 5 ul sample to the lower measurement surface and close the upper arm Quawell Users Manual 2 Click the Measure button to complete all measurements and calculations Nucleic Acid Lm 4 m Help n 5 25 2010 10 14 18 AM Abs 10mm ps 750 SampleID My Sampe 8 70 0 SampleType EC asDNA lt o 65 0 60 0 Abs 10mm 42754 _ 260nm Abs 10mm 42 754 em 280nm Abs 10mm 27 43 40 0 260 280 1 558 350 260 230 f ng ul 2137 699 20 0 340 Baseline 240 250 260 270 280 290 300 310 320 330 340 350 380 Multi Spectra Blank Clear Data Save Graph 0 8 Sample Wave Length 230 260 280 260 230 Concer 8 dsDNA 42 754 42 754 27 43 1 558 1 2137 699 Sample 7 dsDNA 230 37 102 83 156 57 01 1 458 2 241 4157 8 My Sample 6 dsDNA 230 0 046 0 026 0 006 4 333 0 565 1 288 5 dsDNA 0 013 0 001 0 013 0 077 0 077 0 05 4 dsDNA Anm AMA AMA 11347 1499 3 Lift the upper arm and remove the sample from both upper
24. sorbance value and the X axis is for the wavelength The maximum absorbance value is automatically adjusted to the highest absorbance value The user is also allowed to manually enter the absorbance value Sample ID there are two windows that display the sample ID information The left window displays the sample ID input by the user The default is My Sample The right window displays the ID number for that sample ID The ID number begins at 1 and increases by 1 for each measurement Changing the Sample ID will automatically reset the ID number to 1 Sample Type chose the sample type for the sample to be measured There are 4 different sample types for protein measurement 1Abs 1mg ml BSA IgG and Lysozyme The default is 1Abs 1mg ml The sample type is disabled after blanking The extinction coefficient of the sample selected will appear in the EC window SW and Abs 10mm the user selected wavelength and corresponding absorbance The user selected wavelength and absorbance are not utilized in any calculations Ads260 10mm absorbance of the sample at 260 nm Abs280 10mm absorbance of the sample at 280 nm 260 280 ratio of the absorbance at 260 nm and 280 nm mg ml sample concentration in mg ml 22 Quawell User s Manual Measurement Range BSA Absorbance accuracy Concentration Reproducibility 1mm 0 05 100 mg ml 296 Abs 0 0 2 0 002abs Abs 0 2 1 0 02abs
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