Home

Biomek® 2000. Hardware and Software

Contents

1. 3 1 Installing the Side Modules esee enne 3 1 Installing Labware Tip Rack and Collar Holders ses 3 3 Labware Hold ts 1 2 ene e iu e erae e ie ro oo ied 3 3 Tip Rack Holders iu as Weenie een dak RR eae ORO de 3 3 CollarHold rssic cssc E e onte a e ete en alert 3 4 Installing Tool Racks 32 EE e e etre rend 3 6 Installing Racks tuf ette booa 3 7 Installing the 96 Filtration System eese 3 8 Installing the Support Block for the Wash Tool Reservoir 3 10 Installing BioWorks Software Badia e MTM Mc 4 1 Installation Proce dE be C E HE HEUS sare erai 4 BioWorks Program Group eese eene ener nennen enter entes 4 15 Overview of Alignment MWhen to Align neni d e ee oen a dete rest teen epa sites 5 1 Side Loader niece en e ed pe e lee RE RR a 5 2 M 5 2 Accessing the Alignment Functions eene 5 3 Alignment Control Screens 1 eise eee mesitestei ettet eee ene toe 5 4 The Move X Y and Z Axis nee nennen 5 5 Worksurtace Alignment teretes nte baee ten edet eet ree een eere eee 5 5 Sside Loader Aligninent in ende Oe EU OR ete oT 5 6 Gripper Alignment ITE Eie sla 5 8 Increment Values hee uH eue 5 10 The Position Value
2. Biomek 2000 3 3 Hardware and Software Installation Guide Chapter 3 Document 609903 Figure 8 Orienting Tip Racks ina Labware Holder Note that you must remove the lid from the tip rack before attempting to use the tips If you have an optional Gripper Tool or Side Loader the lid can be removed automatically and discarded by the tool when the first tip from the rack is needed so long as the tip rack is in an accessible location When using the Side Loader or Gripper Tool to remove tip rack lids you should first loosen the lid by hand especially for sterilized tips to facilitate later removal Refer to the BioWorks Software Reference Manual for additional information Collar Holders Collar holders look similar to Labware Holders with the addition of two pins which are used to position the vacuum manifold collar The collar holder is specially designed to hold a manifold collar on the worksurface when the collar is not in use See below To install a collar holder on the worksurface insert the locating pins on the bottom of the holder into the corresponding holes in the worksurface with the Front label facing forward Biomek 2000 Hardware and Software Installation Guide Document 609903 Figure 9 Installing the Collar Holder To place a manifold collar on the Collar Holder line up the pins on the Collar Holder with the holes on the bottom of the collar as shown below You can also use a colla
3. E eee E ix Side Loader 9SLD A Te EXTR Ea a a X SE Incubatot 2 noe t He rl HE PR eee derer ves EE X High Density Replicating System sss eterne enne 1 Plate R ader dieere Hn DNI Re EU xi Wash Systemerne ana ette tette ere tre ere e ette pee toe Hte vr etes ea xii Heatei Cooler Block ere b o eerte RE A Ee e TU Io RARI RS xii Biomek 2000 Safety Features esses xii Summary of Warning Information esseeseseseeeeseeeneenenee enne enne entere nent enne xii Introduction OVERVICW EE 1 1 General System Guidelines cie ete me tepore 1 1 Electrical Requirements iii tee La e t ee e Uo cepe nia 1 4 Weight and Space Requirements eese rennen 1 4 Computer Requirements 2 teet te ene etr en eb 1 5 Vacuum Requirements iade ende eiea edet eee dee Cete Ue eei Ce ie e e ete 1 5 Unpacking and Moving the Biomek Unpacking tet eet edere ett e Hs e cadets i Pe Eee eet e e ees 2 1 Moving the Biom k 5 n Genet oe ice ue reb inp cte 2 1 Connecting the Components eerte dn tein etre ere red eoe ovde 2 2 Biomek 2000 i Installation and Operations Manual Table of Contents Document 609903 AA Installation Installing Worksurface
4. which will guide you through the rest of the setup process E Biow orks 3 0 Setup is preparing the InstallShield Wizard Please wait If you should attempt to un install the software the following screen may appear 6 Click OK to proceed 7 Next the Welcome screen should appear as shown on next page 4 2 Biomek 2000 Hardware and Software Installation Guide Document 609903 BioWorks 3 0 Setup BioWorks 320 Welcome to the BioWorks Setup program This program will install BioWorks on your computer It is strongly recommended that you exit all Windows programs before running this Setup program Click Cancel to quit Setup and then close any programs you have running Click Next to continue with the Setup program WARNING This program is protected by copyright law and international treaties Unauthorized reproduction or distribution of this program or any portion of it may result in severe civil and criminal penalties and will be prosecuted to the maximum extent possible under law Next gt 8 You will be prompted to enter your name company name and the serial number of the instrument The serial number is embossed on the nameplate on the rear or the instrument Click Next when you are finished Biomek 2000 Hardware and Software Installation Guide Chapter 4 Document 609903 User Information Type your name below You must also type the name of the company yo
5. Advance when in place Click OK to acknowledge the prompt Left Side Module Alignment xY zZ A Back Up lt gt Left Right e E Down Front Jolie Increment 100 E Y 100251 Test 7 100 E 01 1 x 0 00 y 0 00 z 0 00 Use the direction buttons to move the probe to the target then click Advance on the Left Side Module Alignment dialog to proceed 5 20 Biomek 2000 Hardware and Software Installation Guide Document 609903 You will see the following prompt BioWorks Diagnostics x Leave the Labware Holder in 41 Click OK to proceed The alignment probe moves to the opposite corner of the labware holder Note that there is no target at this position Worksurface Alignment Safe to move down 3mm If it is safe to move the probe click Yes to proceed BioWorks Diagnostics Ed Move down till the alignment probe almost touches the labware holder click on Advance when in place Click OK to proceed Use the movement controls to move the probe down until it just touches the surface of the labware holder Again use a piece of paper to check the height of the probe BioWorks Diagnostics Ed Move to the alignment point click on Advance when in place Click OK to acknowledge the prompt Biomek 2000 5 21 Hardware and Software Installation Guide Chapter 5 Document 609903
6. DO NOT POWER DOWN Workstation blocks left 874 blocks left 736 blocks left 600 blocks left 460 blocks left 316 blocks left 174 blocks left 39 Flash Bank1 done Ill SAFE to POWER DOWN NOW II FLASH Download complete WS getting file BANK1 LZS For Help press F1 1 When the download is finished a message appears to notify you that the process has completed successfully and that it is safe to turn off the workstation BioWorks Diagnostics x Remember to recycle the workstation power 2 Exit the Diagnostics module and close Workstation Server 3 Turn off the Biomek workstation wait at least 60 seconds then turn it back on Biomek 2000 7 3 Hardware and Software Installation Guide Chapter 7 Document 609903 4 On the computer exit Windows and restart Windows to load the configuration changes 5 Restart the Workstation Server and establish the connection between the PC and the workstation by clicking on the icon from the BioWorks Program Group 7 4 Biomek 2000 Hardware and Software Installation Guide Document 609903 Chapter Eight Sales and Service Offices SS General Information Please use the following address and phone number for all inquiriesin the U S Outside of the U S please contact your local Beckman office Beckman Instruments Inc 2500 N Harbor Blvd P O Box 3100 Fullerton CA 92834 3100 Sales 1 800 742 2345 Service 1 800
7. Tool Release Button 2 Alignment Probe 3 P20 Tip Before you begin you must install the alignment probe and tip Push a P20 tip onto the probe Then install the alignment probe by pressing the Tool Release Button and pushing the alignment probe onto the plunger Make sure that the arrow marked Front is pointing towards the front of the Biomek worksurface Release the button when the alignment probe is properly seated Aligning the Worksurface The worksurface is aligned in modules Depending upon which modules are present on your system you will align the Base Module and one or more of the following Left Side Module e Right Side Module e Plate Reader if installed on the left side of the Biomek worksurface You should begin with the Base Module alignment then align the remaining components as appropriate 5 12 Biomek 2000 Hardware and Software Installation Guide Document 609903 Aligning the Base Module Note You should perform a Position Calibration prior to any work surface alignments To align the Base Module begin by selecting Align Worksurface Base Module from the Diagnostics menu bar This displays the Base Module Alignment dialog Base Module Alignment A Back 5 Up a li Left Right v Front XY N Increment 100 5 Y 100 5 7 100 5 Bi Cancel 0 00 z 000 Click Start to begin Worksurface Alignment
8. WRIST refers to the angle in the horizontal plane between the center line of the hand and the center line of the arm Use these vectors to position the alignment sight so that the quarter circles on the sight line up with the quarter circles on the target to create a full circle The target and sight should form two complete circles with no gaps showing at any position Keyboard Support for Alignment You can use the keyboard and numeric keypad for commonly used functions during alignment of the Side Loader This is especially useful when aligning positions on the Side Loader stack areas 2 and 3 Notes Turn NUM LOCK OFF before using the keys Grip buttons act as toggles either one will open or close the grip Biomek 2000 6 3 Hardware and Software Installation Guide Chapter 6 Document 609903 Decrease Increme Value Increas Increme Value 900068 Al DO Common Command Buttons Use the Save button to save the current alignment data to the workstation Use the Cancel button to discard the last set of alignment values and continue using the previous settings Rotation and wrist movements right and left are relative to viewing the target from above from your working position Biomek 2000 Hardware and Software Installation Guide Document 609903 The Side Loader Warm Up Routine Note Limits of Travel Calibration should always be performed before SL Alignment In the Diagn
9. including the expansion area to prevent obstructing the movement of the instrument e Always check the position of the tools and labware before beginning any stage of the method to ensure that their locations on the worksurface match those given on the window Also check that the labware is properly seated on the worksurface before beginning an operation Cleaning You may clean the spill trays of the Biomek worksurface and the nozzles of the Wash tools Please observe the following precautions e Be careful when handling the Wash tools as the nozzles are sharp e Contact your laboratory safety officer and refer to the guidelines in the section titled Chemical and Biological Safety if you will be cleaning spill trays that may have been exposed to hazardous solutions Maintenance Turn the POWER off and UNPLUG the Biomek before changing fuses or performing any maintenance Do NOT autoclave Pipetting or Wash tools autoclaving may cause damage to the internal parts Perform only the maintenance described in this manual and in the Biomek 2000 Maintenance and Troubleshooting Guide Mainte nance other than specified in these manuals should be performed only by Beckman trained qualified personnel It is your responsibility to decontaminate components of the Biomek before requesting service by a Beckman Field Service Representative or returning parts to Beckman for repair Beckman will NOT accept any items which have not been decon
10. x Clear the W orksurface and place a Labware Holder at amp 2 i Cancel You are prompted to clear the worksurface and place a labware holder at position A2 Click OK when done Biomek 2000 5 13 Hardware and Software Installation Guide Chapter 5 Document 609903 BioWorks Diagnostics Place the alignment probe on the head You are prompted to install the alignment probe When it is installed click OK Worksurace Alignment A 9 H Safe to move down 3mm The alignment probe will be automatically positioned over the target area at position A2 or close to it Verify that there is nothing obstructing its movement and that it is fairly close to the alignment target The probe should not be over one of the posts on the labware holder or anything else higher than the surface of the labware holder This also prevents the tip from being forced against the worksurface if the z axis alignment is too low When it is safe to move the probe down click Yes Bio orks Diagnostics Move to the alignment point click on Advance when in place You are prompted to position the probe Click OK to acknowledge the prompt Use the direction buttons on the alignment dialog to position the tip of the probe over the hole in the target 5 14 Biomek 2000 Hardware and Software Installation Guide Document 609903 Base Module Alignment Increment art x 10024 Adv
11. Calibration Plate from front to back Gripper Alignment Ea Finger Tip alignment will be performed Please observe the Gripper The front side of the Gripper has two fingers which may not be exactly parallel to the edge of the Calibration Plate If these two fingers are not the same distance from the Calibration plate then use the finger farthest from the plate for adjustment Leave a slight gap between the plate and the finger pad so that you can visually check the alignment Open and Close the Gripper hand using the screen controls as needed to check the alignment To test the alignment lift and replace the plate to make sure that the fingers are closing properly With the grip closed lift and replace the plate in the holder with no visible shift in the y position Use the Z axis controls to align the tips of the Gripper fingers with the edge of the Calibration Plate 6 40 Biomek 2000 Hardware and Software Installation Guide Document 609903 The fingers must be aligned so that when the hand is closed the tips of the fingers press against the SIDE of the plate but do not slide under it The fingers should be centered vertically on the sides of the plate Figure 22 Correct Positioning of Gripper Fingers When you have completed the alignment click Next Finger Tip Alignment Pick up Test The following prompt appears Gripper Alignment Finger Tip alignment will be perfor
12. for Alignment Sight Target Holder 900070 CDR Figure 20 Side Loader Alignment Tools 6 2 Biomek 2000 Hardware and Software Installation Guide Document 609903 These tools are used as follows The Target Holder is used ONLY at the stack locations for position 1 which corresponds to a bottom shelf Do not use the holder for alignment at the top shelf at 1A8 or for Plate Reader alignment Place the Target on top of the Target Holder when aligning at a stack area Use the Target for alignment at locations on the worksurface and the Plate Reader Place the Target so that the curved edge is towards the outside when on a stack area and towards the left when on the Biomek worksurface The arrow points towards SL arm The Sight is always picked up from the Target and Target Holder at location 1A1 and delivered to the same Target and Target Holder in another stack location or to the Target in a worksurface location e Each position to be taught has four vectors ROTATION REACH VERTICAL position and WRIST ROTATION and REACH are polar coordinates ROTATION refers to the direction or angular position of the arm with respect to Stack 1A1 and REACH refers to the distance from the center of the tower VERTICAL refers to the elevation of the arm with respect to the top surface of the Stack Area Target in stack position 1 AT Vertical values are incremented for each step that the arm moves down
13. then place the mounting plate onto the side module as shown below The top of the spill tray should be flush with the top of the side module Figure 6 Placing the Mounting Plate onto the Side Module 3 2 Biomek 2000 Hardware and Software Installation Guide Document 609903 Installing Labware Tip Rack and Collar Holders Labware Holders Labware holders are used to hold plates 24 place tube racks and reservoirs on the worksurface To install a labware holder on the worksurface insert the locating pins on the bottom of the holder into the corresponding holes in the worksurface with the Front label facing forward Figure 7 Installing the Labware Holder on the Worksurface To install labware in the labware holder push the labware into the holder so that it seats firmly on the base of the labware holder with labware position A1 in the upper left hand corner Be sure that the labware is registered in the right and top sides of the labware holder Tip Rack Holders To install the tip rack holder insert the locating pins on the bottom of the holder into the corresponding holes in the worksurface with the Front label facing forward To install a tip rack into the holder pull the sliding latch back and firmly seat the tip rack with the Beckman logo facing front as shown below Some tip racks may have a circle engraved around one of the tip positions This position goes in the front right hand corner
14. 551 1150 Fax 1 800 643 4366 For the most current list of parts supplies and accessories contact your local Beckman Sales Office World Wide Web You can also access additional information and find out more about new products for the Biomek 2000 from www beckman com Hardware and Software Installation Guide Chapter 8 Document 609903 8 2 Biomek 2000
15. 6 ees Bill 63 27 io 160 n a 22 5 Ae 10 6 57 27 mi E 33 es 84 Workstation is 17 9in 45cm deep 86 5 SL Interface Plate raises the Biomek 2000 by 2 7in 6 9cm 220 SIDELOADER BASE UNIT 3000 d 79 TWO STACK AREAS t C TABLE i 34 T 86 40 n 40 Biomek 2000 102 PLATE READER INSTALLED IN THE LEFT EXPANSION AREA 6 3 5 6 3 5 16 p THREE STACK AREAS Biomek 2000 READER 83 s5 7 INCUBATORS PLATE READER DIMENSIONS STAND ALONE Length 18 3 in 46 cm Width 13 4in 34 cm 16 in 41 cm with cables protruding from rear Height 7 4 in 19 cm Biomek 2000 Side Loader and Plate Reader Dimensions NOTES 1 R is the minimum clearance radius required for mounting a Plate Reader or SL Incubator on the Side Loader 2 Side Loader dimensions accurate 5 inches w Drawings are not to scale 4 Dimensions with apply to all three stack area drawings Biomek 2000 Hardware and Software Installation Guide Chapter 1 Document 609903 Electrical Requirements Electrical requirements are summarized below Line Frequency 50 60 Hz 50Hz Voltage 100 120 VAC 220 240 VAC Current 5 2 5 Fuses Line Fuses 2 5A 250V 5A 250V Accessory Fuses 2 5A 250V 5A 250V The Biomek 2000 is provided with CENELEC HARMONIZED cord set end of the cord has a molded on cert
16. Align ment tool p n 609809 per instructions provided in the Beckman Gripper Arm Alignment document Publication No 609778 5 2 Biomek 2000 Hardware and Software Installation Guide Document 609903 Accessing the Alignment Functions You must first wait for the workstation server to be loaded While this is being done a screen with a Red Light indicator will appear Once the workstation has been loaded the BioWorks Diagnostic screen with a Green Light indicator will appear BioWorks Diagnostics x Loading the workstation server Green light should be on when ready BioWorks Workstation Server File Diagnostic Log Ei SENEENEENEEENENN o Connection STATE NORMAL 00 01 25 You can start the alignment program using either of the following e Run the Diagnostics program by double clicking the Diagnostics icon in the BioWorks Program Group Then select Align from the menu bar and select the component to be aligned Biomek 2000 5 3 Hardware and Software Installation Guide Chapter 5 Document 609903 From the Edit module select Options Diagnostics from the menu bar This will display the Diagnostics module Then select Align from the menu bar and select the component to be aligned I BioW orks Diagnostics OF x Diagnose View Download Help urface Base Module Side Loader LeteSide Module Position Calibrate H
17. Click Advance on Left Side Module Alignment dialog to continue Left Side Module Alignment A Back Up lt Left Right XY N Vv vx Front Down m Increment x 0024 y 0024 2 0 x 481 00 206 20 26500 Bioworks Diagnostics Place a Labware Holder at B1 Verify that there is a labware holder at B1 then click OK The probe moves to the target at position B1 Worksurface Alignment Safe to move down 3mm 5 22 Biomek 2000 Hardware and Software Installation Guide Document 609903 If there are no obstructions click Yes to continue BioWorks Diagnostics Fa Move to the alignment point click on Advance when in place Use the movement controls to center the probe over the target then click Advance to continue Left Side Module Alignment A Back A Up 4 gt Left Right v N xY m Increment x 0024 10024 z 04 LEE Tet Cancel y 568 90 y 29510 25500 Click OK to save the alignment data You are then reminded to remove the alignment probe when the alignment is complete Biomek 2000 5 23 Hardware and Software Installation Guide Chapter 5 Document 609903 BioWorks Diagnostics Ed Remove the alignment probe from the head Aligning the Right Side Module The alignment procedure for the Right Side Module is the same as that
18. Click on the Stop button on the BioWorks Run window to stop the instrument if it is not an emer gency SL Incubator Operating the incubator at 4 C for longer than twenty four hours may result in ice formation within the chamber adversely affecting temperature equilibrium and assay results Do NOT place the incubator in stack location C as the incubator may inhibit access to the Stop switch of the SL in the event of an emergency Allow enough time for the incubator to reach the desired temperature to ensure reliable assay results typically less than one hour depending on the circulator and desired temperature Use 50 polyethylene glycol and 50 water as the conductive fluid in the water circulator Maximum inlet pressure to the SL Incubator is 5 psig 34 5 kPa When cleaning the incubator wear protective gloves and eyewear Biomek 2000 Safety High Density Replicating System The High Density Replicating HDR System is not autoclaveable nor should you use organic solvents to clean the system However you can autoclave the pins of the HDR Tool and use organic solvents to clean the pins If you are using the bleach tray within the tool holder remove the HDR Tool from the bleach tray and rinse thoroughly after each session Do NOT store or let the HDR Tool stand in the tool holder with its pins immersed in bleach when not in use Extended contact with bleach will result in corrosion of the metal pins Plate Reade
19. align the Gripper Tool after you have done any of the following e Re aligned the worksurface e Replaced the finger pads on the Gripper Tool e Replaced any other part of the Gripper Tool or installed a new Gripper Tool In addition you may need to align the Gripper Tool if you observe errors in labware handling such as dropping lids or difficulty in placing labware in the holder or if a sensing failure occurs Observe the Gripper after a collision to determine if a realignment is needed The Calibration Plate The Calibration Plate provided with the Gripper Tool is shown below The pins are used during replacement of the finger pads and will not be used for alignment 900072 CDR Figure 21 The Calibration Plate For alignment place the Calibration Plate into a labware holder pins up at position B2 on the worksurface You will then teach the Gripper Tool where to find the Calibration Plate and how much grip is needed to just lift it from the holder and hold it above the worksurface Note that during the alignment the Gripper fingers should just touch the edge of the Calibration Plate and not lift it from below The Gripper Tool Alignment Dialog The Gripper Tool Alignment dialog is shown below For a discussion of the Movement Increment and Table of Values fields on this dialog refer to the section titled Alignment Overview in this manual Chapter 5 Biomek 2000 6 35 Hardware and Software Installat
20. as follows 0 5L minute free air flow minimum vacuum at pump 8 psig 55kPa Biomek 2000 Hardware and Software Installation Guide Chapter 1 Document 609903 1 6 Biomek 2000 Hardware and Software Installation Guide Document 609903 Chapter Two Unpacking and Moving the Biomek Unpacking the Biomek In general your Field Service Engineer will unpack the Biomek instrument for initial installation However if you should need to unpack the instrument before the installation appointment use the following procedure Unpack the Biomek 2000 from the shipping container as follows 1 Cut the box restraints and lift the box off to expose the instrument 2 Unbolt the instrument from the shipping pallet 3 Release the bridge and head by removing the stretch wrap 4 Use two people to lift the instrument Grasp the back support and the front rail and lift the instrument onto its leveled destination bench Moving the Biomek If the Biomek must be moved to another location contact your Beckman Field Service representative for assistance Remove the tools from the Biomek and store them in their original cases when not in use This is especially important for the Wash tools to avoid damage to the nozzles If possible use the original shipping pallet and packing material to protect the Biomek during transportation When moving the Biomek DO NOT lift it by the bridge where the pipette head is attached Two people
21. ep ete te lee EG E a a a at 6 15 Stack Areas 2 and te te eet eene ee itte rige etus 6 19 LE 6 19 Sight Drop Off eim eie e et ees eese Hee ne PR ege 6 20 Saving the Alignment 6 20 Plate Reader Alignment i tee ga eee ee det eee 6 22 Aligning Individual Side Loader Stack Areas eee 6 24 Aligning the Side Loader to the Worksurface eee 6 28 Advanced Side Loader Alignment 6 30 uias RH RH ge Hep Hep dede 6 30 Limits of Travel Calibration Function 6 31 IncubatOr rre ste n ee oae se ere Ais 6 33 Gripper Tool Aligenment 2 Eit e EL E e Ere o rere up e eet 6 34 When to Align the Gripper sis tah d npe pbegete ei e 6 35 Th Calibration Plate on He teinte ine E ee RU eene e 6 35 The Gripper Tool Alignment Dialog eene 6 35 Using the Alignment Procedure eese rennen 6 37 Labware Setup eee dee e ete teet petes reote e 6 37 Starting the Program i ee ele odes oH Re e Te ERA HE e ee 6 37 Ysand Z Axas Alignment 5 p eue ertet pee ten a 6 40 Finger Tip Alignment Pick up 6 41 Auto Calibration nete er e blu a e Eee ettet pde 6 44 The Gripper Alignment Status Window eese 6 44 Updating the EPROM General Informations eet
22. for the Left Side Module except that the probe is aligned at positions A6 and B6 Testing the Alignment You can perform a test of the alignment points for the Base Module Left Side Module and Right Side Module by clicking the Test button on the respective alignment dialogs You can also perform an alignment test by selecting Diagnose Align Test from the Diagnostics Window Note You should perform a Position Calibration prior to any Worksurface alignment s For example select Align Worksurface Base Module from the Diagnostics menu The Base Module Alignment dialog is displayed 5 24 Biomek 2000 Hardware and Software Installation Guide Document 609903 Base Module Alignment N Cancel x 0 00 v 000 z 0 00 Click Test to begin You will be prompted to place the alignment probe on the head BioWorks Diagnostics Place the alignment probe the head j Cancel Click OK when completed You will now be prompted to place labware holders at A2 B2 A5 and B5 labware locations Click OK when completed The following message is displayed while the alignment probe is positioned over each of the Base Module alignment points Biomek 2000 5 25 Hardware and Software Installation Guide Chapter 5 Document 609903 Align Test Base Visiting the current Base alignment points You should visually verify them realign if any are incorrect The
23. gook1 d Cancel 13 Click Next to proceed 14 If you are upgrading or re installing the software you can specify the same directory that you used before Your methods and the customized tool device pattern and labware definitions which are used in those methods can be saved and imported into the new version Note Custom definitions which are not used in any methods will not be saved They can be imported after installation if desired 15 You may select the Browse button This will prompt the Choose Folder screen as shown on the next page Biomek 2000 4 5 Hardware and Software Installation Guide Chapter 4 Document 609903 Choose Folder x Please choose the folder for installation Path C BIOWORKS Directories OK E c z BIOWORKS BDOK1 Cancel C3 DDETest E3 FLOPPY C INITDB Drives Poos Ff 16 If the installation program detects alignment files from a previous installation you will see the following prompt Biomek Alignment Files x Alignment files already exist Do you want to overwrite your existing alignment files Overwrite qe eer lt Back Cancel Click Next to proceed This screen will not appear if this is a first time installation Biomek 2000 Hardware and Software Installation Guide Document 609903 Click Yes or No in the Overwrite box depending on whether or n
24. installation was successful as shown on the next page This screen will also present you with the option of viewing the Readme file which contains information not available in the printed manual Biomek 2000 4 13 Hardware and Software Installation Guide Chapter 4 Document 609903 Software Installation Successful The Bio Works software installation was successful The ReadMe file contains important information that is not available in the printed manual View Readme Click Finish to complete Setup Click Finish when the Setup is complete 4 14 Biomek 2000 Hardware and Software Installation Guide Document 609903 BioWorks Program Group The software installation program automatically creates the BioWorks Program Group similar to the one shown below Biow orks Of File Edit View Help ew BioScript Biow arl z Advisor amp E dit Lab Book Readme Manager 4 S A Run Workstation System Config Server 1 object s selected 333 bytes A If this is a new installation you will probably receive on site training at this time from the field Service engineer who has performed the installation If you are a new user and will not be receiving on site training at this time refer to the Biomek 2000 User s Guide and Tutorial Manual for information and instructions on how to use each of the BioWorks modules The Biomek 2000 User s Guide provides various step by step ex
25. labware with unusual requirements If a change in grip force will require a Limits of Travel Calibration followed by a check of the alignment points on the Side Loader If the grip force setting is changed then a Limits of Travel Calibration MUST be performed Subsequently you must perform a check of the alignment points on the SL 6 30 Biomek 2000 Hardware and Software Installation Guide Document 609903 Grip Force It BioW orks Diagnostics Diagnose View Download Help Worksurface Side Loader Configuration Position Calibrate adig al Gri Stack Area 1 xinpper Stack Area 2 Exit Stack Area 3 SL Worksurface SL Warm up Grip Force Limits of Travel Calibration Incubator Advanced gd ee Limits of Travel Calibration Function Note You should always perform the Limits of Travel Calibration before performing an alignment of the Side Loader Select Align Side Loader Advanced Limits of Travel Calibration from the menu bar at the top of the Diagnostics Dialog Biomek 2000 6 31 Hardware and Software Installation Guide Chapter 6 Document 609903 Limits Of Travel Calibration WA BioW orks Diagnostics Diagnose View Download Help Worksurface Side Loader Configuration Position Calibrate Autoalign Gri Stack Area 1 Aper Stack Area 2 Exit Stack Area 3 SL Worksurface SL Wa
26. menu bar at the top of the Diagnostics Dialog Allow 5 to 10 minutes for the process to complete 3 Then select Align Side Loader SL Worksurface from the menu bar IA BioW orks Diagnostics Diagnose View Download Help W orksurface Side Loader Configuration Position Calibrate di Gi Stack Area 1 SITES Stack Area 2 Exit Stack Area 3 SL Worksurface SL Warm up Advanced SL Worksurface Biomek 2000 6 29 Hardware and Software Installation Guide Chapter 6 Document 609903 Place the workeurbace target at A4 4 Place the worksurface target in position A4 as prompted by the software Click OK 5 Align the sight with the target in position A4 At each position use the Home amp Back button to test the alignment then use the Advance button to advance to the next position 6 Move the target to A5 and align the sight When aligned click Advance 7 Repeat the alignments with the sight and target at positions B4 B5 and Click OK at the end of the sequence and save the data as described above Advanced Side Loader Alignment Options To access these options select Align Side Loader Advanced from the menu bar on the Diagnostics window Grip Force The Grip Force function is used to adjust the force used to pick up labware with the Side Loader In general you should not change the setting unless you are working with some non standard
27. splashing of liquids therefore take appropriate safety precautions such as the use of safety glasses when working with potentially hazardous liquids Use an appropriate containment environment when using hazardous materials Observe the appropriate cautionary procedures as defined by your safety officer when using flammable solvents in or near a powered up instrument Make sure that the vacuum is attached prior to any method that contains any wash or aspirate functions Moving Parts The bridge and head assembly may suddenly move To avoid injury due to moving parts you must observe the following Never attempt to exchange labware reagents or tools while the instrument is operating as indicated by the amber safety light at the end of the bridge When the light is on the workstation is in operation and may move suddenly and rapidly at any moment A beep sounds just prior to initial movement to provide additional warning Never attempt to physically restrict the movement of the head and bridge assembly Use the Stop switch at the base of the workstation if an emergency stop is required Pressing this switch will freeze the workstation but not the Side Loader Click on the Stop button on the Run window to stop the instrument if the stop is not an emergency viii Biomek 2000 Safety e Because the parts of the Biomek move automatically keep clear of the head assembly during operation Also keep the area around the workstation clear
28. the alignment data just collected and test the alignment points 5 18 Biomek 2000 Hardware and Software Installation Guide Document 609903 Finally remove the alignment probe from the head by clicking ok on screen shown below BioWorks Diagnostics x Remove the alignment probe from the head Aligning the Left Side Module To align the Left Side Module begin by selecting Align Worksurface Left Side Module from the Diagnostics menu bar This displays the Left Side Module Alignment dialog Click Start to begin Worksurface Alignment Ed Clear the Worksurface and place a Labware Holder at Cancel Make sure that the worksurface is clear and install the Labware holder at A1 Click OK You will be reminded to install the alignment probe Make sure that the alignment probe is installed and click OK The alignment probe will move to the target area at position Al or close to it Verify that there is nothing obstructing its movement and that it is fairly close to the alignment target The probe should not be over one of the posts on the labware holder or anything else higher than the surface of the labware holder Worksurface Alignment 7 Safe to move down 3mm Biomek 2000 5 19 Hardware and Software Installation Guide Chapter 5 Document 609903 When it is safe to move the probe down click Yes BiowW orks Diagnostics Move to the alignment point click on
29. the Plate Reader tray Place the warksurface target in the Plate Reader tray at 1F1 Skip Help 6 Make sure that the Plate reader tray drawer is open completely then place the Target in the tray Click OK then align the Sight to the Target Click Advance when you are done Aligning Individual Side Loader Stack Areas If you have fewer than three Side Loader stack areas or if you only want to align one part of the Side Loader system you can use the following procedure 1 Be sure to perform a Side Loader warm up before proceeding 2 Always perform a Limits of Travel Calibration before doing any alignment procedure Select Align Side Loader Advanced Limits of Travel Calibration from the menu bar at the top of the Diagnostics Dialog Allow 5 to 10 minutes for the process to complete 6 24 Biomek 2000 Hardware and Software Installation Guide Document 609903 Align Side Loader Advanced Incubator Limits of Travel Calibration Biomek 2000 6 25 Hardware and Software Installation Guide Chapter 6 Document 609903 3 Then select the area that you want to align from the menu bar Select Align Side Loader then select one of the areas shown Stack Area 1 Stack Area 2 or Stack Area 3 I BioW orks Diagnostics OF x Diagnose View Download Help Worksurface Side Loader Configuration Position Calibrate aura Stack Area 1 Grip
30. with the default values In general you should only overwrite the alignment data if you will be performing a complete realignment of the entire system including the Side Loader and any other options the serial port on the computer which connects to the Biomek the ports on the Biomek that you are using for accessories such as a Plate Reader Side Loader or other device the accessories that you are using If you are installing an update to the software the procedure is virtually the same except that you will also be prompted regarding saving your previous lab books and custom libraries methods and labware Installation Procedure To begin the software installation process do the following 1 Ensure that your computer meets the minimum requirements and uses Windows NT as the operating system Record the serial number of your instrument embossed on the tag on the left side of the front rail and on the Nameplate on the back panel of the Biomek You will need to enter it during software installation Biomek 2000 4 1 Hardware and Software Installation Guide Chapter 4 Document 609903 3 Save all relevant data and close out any other BioWorks or BioScript applications currently running on the workstation PC 4 Insert the BioWorks 3 0 Installation CD ROM in the CD drive of the computer 5 The Install Wizard program will automatically initiate the installation process The Setup screen should appear Setup
31. 33 Hardware and Software Installation Guide Chapter 6 Document 609903 Pa BioW orks Diagnostics mel 1 Diagnose View Download Help Worksurface Side Loader Configuration Position Calibrate Buto align ET Stack Area 1 Stack Area 2 Exit Stack Area 3 SL Worksurface SL Warm up Advanced Grip Force Limits of Travel Calibration Incubator Incubator Gripper Tool Alignment The alignment procedure for the Gripper Tool determines the following reference settings and stores them on the workstation the correct position for the Gripper with respect to the y axis front to back position on the worksurface the correct position for the Gripper with respect to the z axis up and down sets the width calibration for the tool This is the correct fingertip spread for gripping labware items at the side as opposed to gripping from under the labware Because many of the positions determined during the alignment procedure are relative to worksurface positions you must align the worksurface before aligning the Gripper Tool 6 34 Biomek 2000 Hardware and Software Installation Guide Document 609903 When to Align the Gripper The Gripper Tool must be aligned when it is first installed on a system In addition alignment of the Gripper Tool may be affected by a variety of factors including wear on the finger pads and labware collisions You should
32. B Auto Plasmid 644 Auto Plasmid 54B Auto Plasmid 96 Delete Demo Install gt gt Auto Plasmid 48B Auto Plasmid 644 Auto Plasmid 54B Auto Plasmid 96 Demo m Exit Help Install All gt gt Note If you change standard method but do not save under a new name it WILL be overwritten unless you de select this method Click Continue when you are finished Biomek 2000 4 11 Hardware and Software Installation Guide Chapter 4 Document 609903 25 If you have a previous version of BioWorks on the system you can choose which existing Tool libraries to save and update to the new system Click on the appropriate Tool libraries Available Tools on the following screen then click on Install To upgrade all Tool libraries click on Install All Install Tools Available Tools Installed Tools Install gt gt Install All gt gt MP20 deliver 20 repeat END Bee 200 blowout MP200 blowout Exit Help Fan Gripper HDRT HDRT384 MP20 Note If you change a standard method but do not save it under a new name it WILL be overwritten unless you de select this method Click Continue to proceed 26 If you have a previous version of BioWorks on the system you can choose which existing Labware libraries to save and update to the new system Click on the appropriate Labware libraries Available Labware on the following screen th
33. Beckman Part No 609903 AA April 1998 Biomek 2000 7 Hardware and Software Installation Guide BECKMAN Beckman Instruments Inc 2500 N Harbor Blvd Fullerton CA 92834 3100 Copyright 1998 Beckman Instruments Inc Printed in U S A Copyright Licenses and Trademarks Copyright Beckman Instruments Inc 1998 All rights reserved No part of this publication may be reproduced transcribed transmitted or translated into any language in any form by any means without the written permission of Beckman Instruments Inc The product warranty does not apply to software which is warranted on an as is basis without fitness for any specific purpose with the exception of the media which is warranted against defects in materials and workmanship for a period of one year The software is copyrighted and may not be altered or given to a third party without the written authorization from Beckman Instruments Installation and Operations Manual Document 609903 AA SSS hl Notice Safety Notice Safety During Installation Electrical Safety e none tee esie d det ee diee vii Safety Against Risk of Fite oed ge dr e Rei etur cocta viii Chemical and Biological Safety nennen viii Moving Parts eue ete eni gU RD RE HG TU EUIS viii Cleaning on tete ete tan Re e eH eot cepe inta ix Maintenance ioo ese PERPE ien es ix ACCeSSOLy Safety
34. EMP command may not permanently turn off the block if a programmed event list is executing Biomek 2000 Safety Features The Biomek 2000 workstation is equipped with several safety features A Stop switch is located on the front of the instrument Press this switch to stop the Biomek Also the warning light at the top of the bridge warns you that the instrument is operating and emits a beep before the initial movement in a method When this light is on do NOT attempt to change labware Summary of Warning Information This manual is provided to help you establish safe conditions for performing the maintenance and servicing of your equipment Specific considerations and precautions are also described in the manual but appear in the form of Warnings Cautions and Notes It is important that you service your equipment in accordance with this instruction manual and any additional information which may be provided by Beckman Address any questions regarding the safe and proper maintenance and servicing of your equipment to your nearest Beckman Sales and Service Center Xii Biomek 2000 Hardware and Software Installation Guide Document 609903 Chapter One Introduction Overview The Biomek 2000 Laboratory Automation Workstation is designed for applications that require rapid accurate and reproducible liquid handling operations The Biomek is intended for installation by a trained Beckman field service engineer therefore this
35. F1 The Gripper Alignment Status window displays the progress of the alignment and calibration procedures and prompts you to save the alignment data to the workstation 6 44 Biomek 2000 Hardware and Software Installation Guide Document 609903 Chapter Seven Updating the EPROM General Information If a software upgrade is performed on your system you may need to update the information on the EPROM inside the Biomek 2000 Instrument Refer to the Release Notes or the README file provided with the software upgrade to determine whether or not you need to do this CAUTION The Flash EPROM Update process takes approximately 10 15 min utes During this time the computer and the Biomek must remain on connected and undisturbed and the process must not be interrupted before it runs to completion If the download process is interrupted for any reason the EPROM chip in the Biomek may be permanently damaged and will require service from a Beckman Service techni cian If an EPROM update is required use the following procedure 1 Install the new software as instructed in the section titled Installation in this manual Chapter 3 2 Restart the computer to complete the load of the new software and power up the Biomek 3 Close any other open applications before you start the update process 4 Double click the Diagnostics icon from the BioWorks program group to start the Diagnostics module This should also initial
36. Gripper Alignment x e Is the Base Module already aligned 5 8 Biomek 2000 Hardware and Software Installation Guide Document 609903 Gripper Alignment Gri m Back A lt 4 gt Up Open Left Right v v Down Close Front N zu 5 Speed Normal Slow Motion M Log IL 50 VE Cancel Sense Point w 0 00 0 00 2 0 0 0 00 The X movement has effect on alignment The Y Direction button moves the Gripper Tool in the corresponding direction on the worksurface For an operator located in front of the Biomek the y axis corresponds to front to back The Z Direction buttons move the Gripper Tool vertically Grip buttons open and close the fingers of the Gripper Tool and are also used to test the adjustment of the grip position The distance is controlled by the T value Biomek 2000 5 9 Hardware and Software Installation Guide Chapter 5 Document 609903 Increment Values The Increment values or distance values for the Side Loader allow you to select the step size for each direction In general you will select the step sizes first and then the direction Step sizes are selected individually for each axis then you use the direction buttons to move in each axis You can set values to higher numbers for larger steps to speed up rough alignment then select a smaller step value to make more pre
37. Support 6 Brdge 3 Side Module both sides 7 Safety Light 4 Front Rail Table 1 Biomek 2000 Laboratory Automation Workstation Caution Do not lift the Biomek 2000 by the bridge Two people are needed to lift the Biomek grasping the front rail and the back support Space requirements for the Biomek 2000 the optional Side Loader the optional Incubators and the optional Plate Reader are shown on the following page Also allow additional space above the Biomek for servicing approximately 1 foot or 0 5 meter 1 2 Biomek 2000 Hardware and Software Installation Guide Document 609903 ONE STACK AREA BIOMEK 2000 WORKSTATION B4 gt 55 137 48 1 i19 T SSeS 122 ug y as 2000 4 5 11 17 9 pat 569 a 1 l IL i 485 s 123 12 9 m SN 129 33 AN Li 215 L3 55 VA M e NEC ur on I NS ND M 10
38. amples and tutorials to help you learn the most frequently used functions of the Biomek and of BioWorks software Note Before running any methods after a new software installation be sure to restart the computer If this is a new system or if you did not save the previous alignment files you must also perform a worksur face Gripper and SL alignment as described in the next section Installing the Bio Works 3 x software on a Windows 95 platform will only install the Edit module and will not be officially supported by Beckman Instruments Inc Biomek 2000 4 15 Hardware and Software Installation Guide Chapter 4 Document 609903 4 16 Biomek 2000 Hardware and Software Installation Guide Document 609903 Chapter Overview of di Alignment This chapter provides general information on the alignment process and explains some of the functions that are common to the different alignment processes The specific procedures for alignment are provided in subsequent chapters Caution If you use the Emergency Stop switch to abort an alignment proce dure all of the alignment data collected to that point may be lost or corrupted You should re check the alignment or begin the alignment again from the beginning Proper alignment is critical to the performance of the Biomek In general alignment consists of teaching the Biomek exactly where specific points are in space To do this you will use an interactive program to
39. an Sige Module late Reader nipper Exit Alignment Control Screens You will use a screen similar to the one shown on the following page for each of the alignment procedures 5 4 Biomek 2000 Hardware and Software Installation Guide Document 609903 Base Module Alignment 2n Z Back A Up E Left Right v v Front Down m Increment 10025 10025 il z 1001 01 These alignment screens provide some common functions which are discussed below The Move X Y and Z Axis Buttons Move buttons will vary slightly for each of the alignment windows depending on what you are currently aligning Use these buttons to move the probe tip for worksurface alignment the Side Loader arm or the Gripper Tool as appropriate The Position Values change in response to clicking on these buttons See page 5 8 for an example The types of moves that you may perform using the Move buttons are as follows Worksurface Alignment The Worksurface alignment window is shown on the following page Biomek 2000 5 5 Hardware and Software Installation Guide Chapter 5 Document 609903 Base Module Alignment N v Front m Increment X 100 y 1002 z 1002 Di The and Y Direction buttons move the tool head in the corresponding direction on the worksurface For an operator located in front of the Biomek the x axis corr
40. ance Y 100 24 Test z 100 a daa x 708 71 y 247 50 2 265 54 Note The tip of the probe should be at the same height as the labware holder and centered in the alignment target hole To determine the correct height slide a piece of thin paper under the probe When the probe is at the right height the paper should not be punctured how ever the paper should be able to be pulled away with a very snug resistance Click Advance when you have positioned the probe properly Bioworks Diagnostics Place a Labware Holder at B2 Biomek 2000 5 15 Hardware and Software Installation Guide Chapter 5 Document 609903 As prompted place a labware holder at position B2 of the worksurface Click OK The alignment probe will be automatically positioned over the target area at position B2 or close to it W orksurface Alignment Safe ta move down 3mm Verify that the probe is close to the alignment target and free to move down then click Yes BioWorks Diagnostics F3 Move to the alignment point click on amp dvance when in place Click OK to proceed Again use the direction buttons on the alignment dialog to position the tip of the probe at the target Click Advance when you have positioned the probe properly As prompted place a labware holder at position B5 of the worksurface Click OK when done Bio orks Diagnostics Place a Labware Holder at B5 5 16 Biome
41. are needed to lift the Biomek Always lift by grasping the front rail and the back support The instrument should be transported in an upright position Avoid shaking and vibration during transportation When re installing the Biomek DO NOT connect the Biomek to an electrical outlet until the instrument is positioned properly on a level bench The Biomek must be realigned after it has been moved Do not attempt to operate the system before verifying the alignment Biomek 2000 2 1 Hardware and Software Installation Guide Document 609903 Connecting the Components The minimum configuration requirement for the Biomek 2000 controller is a Beckman recommended computer or equivalent Refer to page 1 5 for specific requirements A printer is optional Check with your Beckman representative for the latest information on compatible printers and equipment requirements Note Computers not supplied with the Biomek may not be suitable for use with the instrument Equipment not recommended by Beckman can not be installed and or maintained by our Field Service personnel Optional computer accessories such as a printer should be installed according to the manufacturer s instructions To connect the various components of the Biomek refer to the Figures below and use the following instructions 1 Connect the computer monitor keyboard and mouse as instructed by the computer manufacturer 2 Connect the Biomek Interface Cable part n
42. cise adjustments for a final alignment By using progressively smaller step sizes you can control the precision used for positioning the probe arm or hand Increment 10024 y 108 z 100 T Figure 17 Increment Values Example from Worksurface Alignment The Position Values The Position Values are shown at the bottom of each of the alignment windows These fields show the current coordinates of the alignment probe Side Loader arm and hand or Gripper position as appropriate The values are important to the BioWorks program They also let you confirm that the system is responding to your use of the Move buttons even when nothing seems to be moving y 0 00 v 0 00 z 000 Figure 18 The Position Values Example from Worksurface Alignment 5 10 Biomek 2000 Hardware and Software Installation Guide Document 609903 For the Side Loader alignment the position values follow to the paradigm established on the buttons in the alignment windows The following values are displayed on the screen e Rotation values are incremented for each step that the arm moves towards the Right Values are decremented for each step that the arm moves towards the Left e Reach values are incremented for each step that the arm moves in toward the base Values are decremented for each step that the arm moves out toward the stack or worksurface e Vertical values are incremented for each step that the arm moves up Value
43. crement Start c Normal x 100 C Slow Motion v 10 Z 10 E Log I 1055 Cancel Sense Point 0 70 x 683 00 v n 399 z 0910 12 28 Performing pre alignment sequence Please wait Pre alignment sequence complete Performing Finger Tip alignment sequence Finger Tip alignement is completed Click on Next button to start Auto Calibration Biomek 2000 6 43 Hardware and Software Installation Guide Chapter 6 Document 609903 Auto Calibration The Auto Calibration portion of the alignment procedure does not require any user intervention Results are displayed in the status window as the test proceeds Gripper Calibration Auto Calibration results will be displayed in the Status window When the Biomek and Gripper tool stop moving the alignment screen will be displayed Click OK Click the Save button on the alignment screen to save the data The Gripper Alignment Status Window m BioWorks Diagnostics Bg DIN Setup View Download Help Gripper Alignment Status Finger Tip alignment is complete Click on Next button to start Auto Calibration Auto Calibration results SensorOnPosition 9 788000 SensorOnPosition2 9 790667 SensorOnPosition3 9 792000 Auto Calibration sequence is complete Click on Save button to download data to workstation Downloading alignment data to the workstation Please wait Download complete For Help press
44. d Windows is a trademark of Microsoft Corporation Biomek 2000 Vv Notice vi Biomek 2000 Safety Safety Notice The exclamation mark contained in a triangle is an international symbol displayed to indicate to the user that all safety instructions should be read and understood before installation and operation are attempted When you see the safety notice symbol on the instrument or on one of its accessories you should refer to this manual for specific safety information that applies If the product is used in a manner other than specified in the manual the safety and performance of the equipment could be impaired Any installation or service procedures not described in this manual must be performed by qualified service personnel Safety During Installation Do not lift the Biomek 2000 by the bridge Two people are needed to lift the Biomek grasping the front rail and the back support Do not connect the Biomek to an electrical outlet until it is positioned properly on the bench Electrical Safety To reduce the risks of electrical shock this equipment employs a three wire electrical cord and plug to connect the equipment to earth ground To preserve this safety feature Make sure the matching wall outlet receptacle is properly wired and earth grounded e Never use a three to two wire plug adapter e Never use a two wire extension cord or a non grounding type multiple outlet receptacle strip e Any s
45. elds on this dialog refer to the section titled Alignment Overview in this manual Chapter 5 Click Start 12 Click the Start button on the Side Loader Align dialog The Biomek warning buzzer will sound and the light will come on 6 12 Biomek 2000 Hardware and Software Installation Guide Document 609903 13 You will be prompted to remove any Tools from the head and to clear the worksurface 14 Click OK to proceed 15 You will be asked to clear location 1A1 as the Limits of Travel data collection is about to start Allow approximately five to ten minutes for this process 16 Click OK to proceed 17 Next you will receive the following prompt 18 Click OK when done Biomek 2000 6 13 Hardware and Software Installation Guide Chapter 6 Document 609903 Click OK to proceed 19 Next you are notified that the Limits of Travel Calibration is about to start Allow approximately 5 10 minutes for this process The Side Loader Align screen will appear 6 14 Biomek 2000 Hardware and Software Installation Guide Document 609903 lb bebe be db GA Log 20 If you have enabled the Log as shown above you will see the data displayed in the log area of the dialog while it is collected Stack Area 1 1 Use the Move and Home amp Back controls on the Alignment dialog to position the hand at the Target then Close the hand on the Sight Make sure that the f
46. en click on Install To upgrade all Labware libraries click on Install All 1 4 Minitubes 1 4 Minitubes Install All gt gt 12 x 75 24 insat 12 x 75 24 Install Labware 10 75 160 10 75 160 13 x 100 24 13 100 24 13x 100 96 Delete Available Labware Installed Labware 1 0 Minitubes 1 0 Minitubes Install gt gt 10 x 75 24 10 x 75 24 12x 75 128 12x75 128 13 x 100 96 384 well flat 384 well flat Click Continue to proceed 4 12 Biomek 2000 Hardware and Software Installation Guide Document 609903 27 Next you will be prompted with the option of importing your tool delays overages selected tips offsets and slopes to the new database as shown in the following screen Tools used by Existing Methods Do you want to import your modified tool delays overages selected tips offsets and slopes used in existing methods to the new database Click Yes to import your modified parameters to the database 28 Next you will be prompted with the option of importing your existing labware lid parameters to the new database as shown in the following screen Labware with Lids used by Existing Methods Do you want to import the labware lid parameters used in existing methods to the new database Click Yes to import your modified parameters to the database 29 Next you will be prompted with the screen indicating that the software
47. ere ttr M eite eee bo edet feo te egets 7 1 Sales and Service Offices General Information pce eterno eue de eate te 8 1 World Wide ennemi ni a en aan ie 8 1 Biomek 2000 iji Installation and Operations Manual Table of Contents Document 609903 AA iv Biomek 2000 Notice Notice SS Um This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference with the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the used will be required to correct the interference at his own expense This manual is copyrighted and all rights are reserved This document may not in whole or in part be copies photocopied reproduced translated or reduced to any electronic medium or machine readable form without prior consent in writing from Beckman Instruments Inc Biomek and Beckman are registered trademarks of Beckman Instruments Inc BioWorks and BioScript are trademarks of Beckman Instruments Inc Microsoft and MS DOS are registered trademarks an
48. ervicing of this equipment which requires removal of any covers or panels can expose parts which involve the risk of electric shock or personal injury Refer such servicing to Beckman trained qualified personnel e Do not use any power supply other than the type supplied for the accessory for the Biomek 2000 as appropriate for the country where it is installed Biomek 2000 vii Safety Safety Against Risk of Fire Certain electrical circuits within this equipment are protected by fuses against over current conditions For continued protection against risk of fire replace fuses only with the same type and rating specified Chemical and Biological Safety Normal operation of this equipment sometimes involves the use of reagents which are toxic flammable or biologically harmful When using such reagents observe the following precautions Infectious samples must be handled according to good laboratory procedures and methods to prevent the spread of disease Observe all cautionary information printed on the original solution containers prior to their use All waste solutions must be disposed of according to your facility s waste disposal procedures Liquid transfers may generate aerosols Operate the Biomek in an appropriate enclosure and take all necessary precautions when using biohazardous pathologic toxic or radioactive materials Objects dropped onto plates accidental tool release or other accidental collisions may result in
49. esponds to left to right and the y axis corresponds to front to back The Z Direction buttons move the tool head up and down Hint When adjusting the height of the alignment tool the tip of the tool should be just high enough off the surface to allow a piece of thin paper to slide between the tip and the worksurface Side Loader Alignment The Side Loader Section 1 alignment window must be configured to match your workstation setup as shown on the next page Biomek 2000 Hardware and Software Installation Guide Document 609903 Side Loader Section 1 Biomek 2000 5 7 Hardware and Software Installation Guide Chapter 5 Document 609903 The Reach Back and Front buttons move the arm between the base and the stack or worksurface The Rotation Left and Right buttons move the arm around the stack areas in either a clockwise or counterclockwise direction The Up and Down buttons control the height of the hand above the stack area or shelf Vertical values are incremented for each step that the arm moves down The Wrist Left and Right buttons control the rotation of the Side Loader wrist as opposed to rotation of the entire arm assembly Use Home amp Back to move the arm to its Home position and then come Back to the aligned position Use the Hand button to open and close the hand Gripper Alignment A Gripper alignment window will appear to verify that the Base Module has already been aligned
50. ified IEC 320 c13 connector rated min 250V 6A and the other end is terminated in a certified connector suitable for the country of intended use Warning You must use a grounded electrical outlet Do not connect the Biomek to an electrical outlet until it is positioned properly on the bench Weight and Space Requirements The Biomek and Accessories must be installed on a flat table capable of supporting 75 lbs or approximately 35 kg The table must be level stable and not bow by more than 1 8 inch under the weight of the instrument Allow at least 1 foot 0 35 meters of additional space to the left right and above the workstation for servicing Space requirements will vary for the computer required to operate the workstation 1 4 Biomek 2000 Hardware and Software Installation Guide Document 609903 Computer Requirements The Biomek requires a computer for use as a controller The minimum and recommended requirements for this computer are listed below Minimum Recommended CPU Pentium 133 Pentium 200 Video Resolution 800 x 600 1024 x 768 or better Monitor Size 15 inch diagonal 17 inch diagonal or larger Memory 32 MB 32 MB Disk Space 100 MB free disk space or more depending on number and size of methods CD ROM DRIVE REQUIRED In addition the computer must be running Microsoft Windows NT Vacuum Requirements If you are using the Wash Unit the vacuum requirements are
51. inger pads fit inside each of the three grips on the sight Once the hand is holding the Sight properly make sure that the sight is in alignment with the Target Use the Move buttons to move the sight then use the Home amp Back button to check the alignment position Biomek 2000 6 15 Hardware and Software Installation Guide Chapter 6 Document 609903 2 Click Advance when the Sight can be returned to the alignment position repeatedly using the Home amp Back command 3 You will be prompted with the following screens ATO orks Diagno cs 4 Click OK when done Bio W ork Diagnostics 5 Click OK to proceed 6 16 Biomek 2000 Hardware and Software Installation Guide Document 609903 The alignment at position 1A1 is the most critical alignment position for the entire system Ensure that the Sight and the Target match exactly and that at least three Home amp Back functions can be per formed in succession without a change in alignment For 1A1 and subsequent positions align the Sight to the Target using quarter circles on the sight and the target to form two complete cir cles 6 You will be prompted to perform the same alignment steps at positions 1B1 1C1 1D1 and 1E1 The following prompt is for position 1B1 Place the stack area target at 1B1 Skip 7 Click OK to proceed Note that you can also skip individual positions For best results you should always align carefully a
52. iod of time Use the Previous button to go back to the previous alignment procedure Using the Alignment Procedure Note You must align the worksurface of the Biomek 2000 before you align the Gripper Tool Check and replace worn finger tips prior to align ment refer to Publication No 609337 AB Labware Setup Place labware on the Biomek worksurface as follows 1 Place the Gripper Tool Rack at location A2 2 Place the Gripper in the Gripper Tool Rack at position 4 3 Place the Calibration Plate at position B2 in labware holder with the pins up pins may be on either side Starting the Program You can start the alignment program using either of the following e Run the Diagnostics program by double clicking the Diagnostics icon on the BioWorks Program Group Then select the Align Gripper option from the menu bar or From the Edit module select Options Diagnostics from the menu bar This will display the Diagnostics module Then select the Align Gripper option from the menu bar Biomek 2000 6 37 Hardware and Software Installation Guide Document 609903 Chapter 6 Gripper Alignment za liv N Grip Slow Motion Log Sense Point Increment 100 100 5 N IX IX 10025 50 I3 lt Start Next Gave Cancel quence Please wait Pre alignment sequence complete The f
53. ion Guide Chapter 6 Document 609903 Gripper Alignment xY N i 2 E Back A Open Left Right M v Down Close Front Speed Increment Normal x 1004 C Slow Motion Y 100 34 V Log I 5071 Save Cancel Sense Point Xx 0 00 v 10 00 z 0 00 r o o0 Gripper specific functions are listed below The Speed setting controls the speed used to move from one position to the next The Sense Point is the difference between where the Gripper just grips drops the calibration plate and where the internal pressure sensor activates to indicate that the plate is firmly gripped This field displays the Grip Sense Point measured in millimeters on the Gripper drive motor This measurement determines the sensitivity of the Gripper tool for detection of missing labware or lids when a move is performed 6 36 Biomek 2000 Hardware and Software Installation Guide Document 609903 Use the Test button to test the alignment calibration plate pickup You then repeat the alignment process to determine the point when the tool drops the plate At the end of the test the program will display the percentage change in position from the last alignment data No numerical values willl be determined for the Grip Sense points at this time since this is only a test You can use this information to monitor how much the grip pickup parameters are changing over a given per
54. irst prompt displayed asks you to confirm that the base has been aligned Click OK to acknowledge the prompt If you have not aligned the worksurface cancel the process from the alignment screen when it is displayed Align the worksurface then restart the Gripper alignment sequence 6 38 Biomek 2000 Hardware and Software Installation Guide Document 609903 To begin the alignment click Start on the Alignment screen Gripper Alignment AY 2 Grip Back A M pen v M v Down Close Front C Slow Motion Log Sense Point x 683 00 413 15 z 289 10 1314 Pre alignment sequence complete Next you are prompted to confirm that the labware and tools are installed Verify that the Gripper and the Calibration Plate are in the correct locations then click OK for each prompt The Biomek picks up the Gripper Tool from the Tool Rack and positions it over the Calibration Plate Biomek 2000 6 39 Hardware and Software Installation Guide Chapter 6 Document 609903 Y and Z Axis Alignment The following prompt appears Gripper Alignment N Adjust Y alignment Click an Next button when done Click OK to acknowledge the prompt and return to the alignment screen Using the controls on the alignment screen adjust the position of the Gripper as follows Use the Y axis controls to center the fingers of the Gripper tool over the
55. ize the workstation server and display the corresponding traffic light icon at the bottom of your screen Biomek 2000 7 1 Hardware and Software Installation Guide Chapter 7 Document 609903 5 From the Diagnostics module select Download FLASH from the menu bar FE s hagreatics work edie E alit 6 The following screen will advise you that mouse and keyboard cannot be accessed during download Download FLASH x Download will take about 10 minutes wy During this time the Keyboard and Mouse access will be blocked Do you want to continue No 7 You will see a message similar to the following advising you that you cannot use the computer once the download process begins Do not Power Down until download is complete 7 2 Biomek 2000 Hardware and Software Installation Guide Document 609903 Once you confirm that you want to continue with the Flash update process by clicking Yes you will not be able to use the computer until the update is complete DO NOT TURN OFF THE COMPUTER OR THE BIOMEK UNTIL THE DOWNLOAD IS COMPLETE As the download progresses the log will be updated to show the number of blocks that remain to be transferred IA BioWorks Diagnostics OF X Align Diagnose View Download Help r FLASH Download Log Flash Bank done Ill DO NOT POWER DOWN Workstation Programming Flash Bank1
56. k 2000 Hardware and Software Installation Guide Document 609903 Worksurface Alignment 2 Safe ta move down 3mm Verify that the probe is close to the alignment target and free to move down then click Yes BioWorks Diagnostics Move to the alignment point click on Advance when in place Click Advance when you have positioned the probe properly Click OK to proceed BioWorks Diagnostics Place a Labware Holder at As prompted place a labware holder at position A5 of the worksurface Click OK The alignment probe will be automatically positioned over the target area at position A5 or close to it Worksurface Alignment Sate to move down 3mm Biomek 2000 5 17 Hardware and Software Installation Guide Chapter 5 Document 609903 Verify that the probe is close to the alignment target and free to move down then click Yes BioWorks Diagnostics x Move to the alignment point click on Advance when in place Again use the direction buttons on the alignment dialog to position the tip of the probe at the target Click Advance when you have positioned the probe properly Base Module Alignment m Increment x 100 5 Y 100 5 7 100 x 482 00 25500 451 00 Click OK to indicate that you have completed the alignment The alignment positions will be saved to the workstation You can also click the Test button to save
57. late reader Please enter the directory where the SoftMax software is located at Destination Folder C SOFTMAX lt Back Next gt Cancel 22 If you want to install SOFTmax in a different directory click the Browse option to select the Destination folder for the SOFT max software Click Next when you are finished 23 If you have a previous version of BioWorks on the system the existing Lab Books will automatically be saved and updated to the new system as shown in the following screen Save Existing Lab Books Ed Y our Existing Lab Books Lab Books to be Included BOOK1 BOOK1 NEWBOOK NEWBOOK Up date gt gt Update All gt gt pelete Click Continue to proceed 4 10 Biomek 2000 Hardware and Software Installation Guide Document 609903 Note If you change a standard method but do not save it under a new name it WILL be overwritten unless you de select this method Methods will be saved to the default location c M3io WorksV your lab book name mmddhhmm mmddhhmm is a numerical value assigned as your file name and is defined as month day hour minute 24 If you have a previous version of BioWorks on the system the existing Methods will automatically be saved and updated to the new system as shown in the following screen Install Methods Available Methods Installed Methods Auto Plasmid 324 Auto Plasmid 32B Auto Plasmid 32C Auto Plasmid 484 Auto Plasmid 48
58. lete the adjustment and lower the sight onto the target Use the Home amp Back button to check your position Log Provides a log of the actions and events that have occurred during the alignment process REGRIP button During alignment of the stack areas the arm or hand may collide with an object on the stack area or some other obstacle causing the hand to drop the alignment sight If this occurs use this command to correctly reposition the sight in the grip Manually place the target and sight at 1A1 then click Regrip to regrip the alignment sight at position 1A1 and resume the alignment Aligning the Stack Areas Using Auto Align The Auto Align program provides a convenient method for aligning all sections and locations Note however that if a problem occurs during the alignment process you must start again from the beginning In some cases it may be preferable to align each of the sections individually so that if a problem occurs at one section you do not lose the alignment data for the entire system Note To obtain Correct alignment the Side Loader warm up routine should be run prior to aligning the Side Loader The Auto Align pro gram includes Limits of Travel Calibration Preliminary Functions 1 Perform Side Loader Warm Up Routine 2 Start the Diagnostics program from the BioWorks Program Group 3 Select the Align Side Loader Auto Align command from the menu bar 6 8 Biomek 2000 Hardware and Software Installa
59. lows you to specify which option will be applied to the remaining ports Click Next to proceed 20 Specify the options and accessories that you have on your system on the Workstation Surface Setup dialog Once the software is installed you can also access this dialog from within BioWorks to make any necessary configuration changes Note You must either select the Side Loader Section option for Work Surface Side Loader OR the Left Expansion Area option for the Plate Reader 4 8 Biomek 2000 Hardware and Software Installation Guide Document 609903 Workstation Surface Setup Select your Workstation accessories m Additional Labware Sources Worksurface v Disposal 1 Disposal 2 v Left Side Module Right Side Module LeftStacker Carousel HiahtStackenisarusel v Side Loader Area 1 v Side Loader Area 2 wash Unit Siz Port Valve v Side Loader Area 3 Other Options Plate Reader v HDR System v Plate Reader Location v Vacuum Manifold Auto Latches Side Loader Section lt Cancel Click Next when you are finished 21 If you have selected the Plate Reader option in the Workstation Surface Setup screen then the Softmax directory will appear as shown on the next page Biomek 2000 Hardware and Software Installation Guide Chapter 4 Document 609903 SoftMax Directory x You have configured the existance of a p
60. manual does not necessarily provide all of the information necessary for installation of a Biomek 2000 Rather it provides instructions for all of the procedures which can be performed by a user Note also that installation instructions for the various accessories available for the Biomek are generally provided in the respective accessory manuals Before your scheduled installation appointment you should refer to the Biomek 2000 Pre Installation Guide which provides the following information e Installation requirements and specifications for the Biomek 2000 including space and electrical requirements Achecklist of labware and other items which you should have available before installation Information on operator training General System Guidelines When setting up your workstation computer for use with the Biomek you should observe the following guidelines e Disable all screen saver functions while the Biomek is running a method Turn off any power saver functions on the computer These are usually utility programs that allow the computer to sleep during extended periods of inactivity Such programs may interfere with the ability of the Biomek to communicate with the computer while a long method is running Do not use utility programs for disk space compression Biomek 2000 1 1 Hardware and Software Installation Guide Chapter 1 Document 609903 1 Head Assembly 5 Worksurface 2 Back
61. manually move the alignment tools which are probes or sights to the various positions then save those positions The alignment process will differ depending on the configuration of your system If you are using a minimum configuration of an instrument with no side modules then you will align specific points on the worksurface with the Biomek head using a special alignment tool If your system includes one or more optional side modules then using the alignment tool you will also align the head to points on the side module s When a Side Loader is installed an additional alignment process aligns the Side Loader hand with positions on the stack areas as well as the SL accessible locations on the worksurface This procedure should be performed after worksurface alignment If your system includes a Gripper an additional alignment process aligns the Gripper Tool hand with the positions on the worksurface This procedure must be performed after worksurface alignment When to Align You must align the Biomek Workstation after it has been installed for the first time and whenever it has been moved to a new location In general it is also recommended that you check the alignment once a week during normal operation Biomek 2000 5 1 Hardware and Software Installation Guide Chapter 5 Document 609903 Note If you have upgraded the BioWorks software from a version 2 0 or earlier to version 3 0 then you should realign the entire sy
62. med Please observe the Gripper Click OK to acknowledge the prompt and return to the alignment screen During this part of the alignment the Gripper Tool will repeatedly pick up the Calibration Plate then replace it in the holder Each time it picks up the plate the fingers are opened slightly 0 05 mm farther than the last time You will observe the plate and indicate the point when the Gripper releases it Each time the Gripper lifts the plate the following prompt appears Biomek 2000 6 41 Hardware and Software Installation Guide Chapter 6 Document 609903 Gripper Alignment E 2 lz the Gripper holding the calibration block 7 Observe the Gripper and the plate If the plate is being held properly click Yes If the plate is tilted or slips out of the Gripper click No Note that this may take several attempts before the Gripper releases the plate After the Gripper has dropped the plate and you have clicked No the following prompt appears Gripper Alignment EG Finger Tip alignment is complete Click on Next button to Auto Calibrate Continue the alignment process by clicking OK then click Next You are prompted to verify that the calibration plate is seated properly in the labware holder Click OK if the plate was placed properly 6 42 Biomek 2000 Hardware and Software Installation Guide Document 609903 Gripper Alignment e ae Speed In
63. ment 609903 Figure 3 Connecting the Side Loader Control Module C20 o oq Coe 2000 Hardware and Software Installation Guide Document 609903 Chapter Installation pue SS Ul Installing Worksurface Components Installing the Side Modules Side modules may be installed on the left and right sides of the worksurface for additional worksurface space Each side module provides two additional labware locations Note If you install a side module on the left hand side of the worksurface you cannot attach the optional Plate Reader to the left of the work surface Install the optional side modules as follows 1 Place the locating pins on the side of the worksurface into the corresponding holes in the side module Secure with large socket head cap screws provided with the module using a 3 16 in Allen wrench Figure 4 Securing the Side Module to the Worksurface Biomek 2000 3 1 Hardware and Software Installation Guide Chapter 3 Document 609903 2 Verify the locating hole of the side module is flush with the locating hole on the front support rail see below Secure the front of the side module to the front support rail using the small button head socket screw provided and a 1 8 in Allen wrench Figure 5 Securing the Side Module to the Front Rail Place the spill tray inside the side module
64. n positions 2A1 through 2E1 Note that if you have a Plate Reader installed at 2F1 4 Continue with the same process to align at positions 3A1 3B1 and 3D1 on the third stack area Worksurface 1 After you have completed alignment of the stack areas you are prompted to place the Target in a Labware Holder at location A4 on the worksurface 2 Click OK to acknowledge the prompt then use the SL Align dialog to position the Sight over the Target at A4 on the worksurface 3 Repeat this procedure for positions A5 A6 B4 B5 and B6 Biomek 2000 6 19 Hardware and Software Installation Guide Chapter 6 Document 609903 Sight Drop Off 1 After alignment of the worksurface you are prompted to return the Target and the Target Holder to location 1A1 Biow orks Diagnostics x Place the stack area target at 141 for sight drop off 2 When you click OK the arm will return the Sight to position 1A1 then release it onto the Target Saving the Alignment Data 1 When you have completed the process you will see the following prompt Biow orks Diagnostics Click on OF to save current settings 2 Click OK to acknowledge the prompt 6 20 Biomek 2000 Hardware and Software Installation Guide Document 609903 E 1 bEIbbbb ii completed ending side loader configuration for Section to the workstation Location SL 141 ending side loader configuration for Section2 to the workstati
65. nt before putting the microplate on the drawer Alternatively place a clear sheet such as a Molecular Devices blanking template underneath the microplate when inserting it in the drawer Biomek 2000 xi Safety Wash System e Never run the 6 Port Valve dry Liquid should always run through the 6 Port Valve Failure to do so can result in damage to the valve e Strong acids strong bases oxidizers radioactive and biohazardous liquids should not be run through the Wash Unit Each laboratory must qualify this instrument with its unique application s e Plug any unused port with the plugs provided with the 6 Port valve Otherwise liquid may spill through exposed ports when the 6 Port valve is used e When using a Wash tool make sure a quarter reservoir is installed properly in the support block under the tool Heater Cooler Block Failure to provide cooling water to the Heater Cooler Block HCB will cause excessive and potentially dangerous heating of the assembly DO NOT operate the assembly without adequate water flow 1 to 2 liters minute Full power time out faults may be caused by loss of cooling water Should this occur the Heater Cooler Block assembly may be hot e Always be sure to shut down the HCB as the last command of each heating cooling sequence Include a shut down command in all Biomek routines Provided water cooling is maintained extended cooling operations do not adversely affect block life span The T
66. on ide loade guration for Section3 to the workstation 3 Click OK on the Side Loader Align dialog to save the alignment data that you have just collected Biomek 2000 6 21 Hardware and Software Installation Guide Chapter 6 Document 609903 Plate Reader Alignment 1 To align the Plate Reader for use with the Side Loader you must ensure that the plate reader is in the Side Loader configuration at the beginning of the alignment in Stack F in Side Loader Section 1 or 2 BioWorks Diagnostics Diagnose View Download Help Worksurface Pile ES Configuration Auto align Position Calibrate Stack Area 1 Bripper Stack Area 2 Exit Stack Area 3 SL Worksurface SL w arm up Advanced Highlight Stack Area 1 to start alignment 6 22 Biomek 2000 Hardware and Software Installation Guide Document 609903 2 You will be prompted to place the Plate Reader at 1F1 and place the target on the top shelf of the Mini Stack on top of the Plate Reader 3 Click OK when done Biomek 2000 6 23 Hardware and Software Installation Guide Chapter 6 Document 609903 4 Align the Sight to the Target using the Side Loader Align dialogue Click Advance when the Sight is positioned properly Biowforks Diagnostics Move to the alignment paint click on Advance when in place 5 Next you are prompted to place the Target in
67. ostics menu select Align Side Loader Side Loader Warm Up as shown on the following screen IA BiowW orks Diagnostics Eel xi Diagnose View Download Help Worksurface Side Loader Configuration Position Calibrate edes Stack rea 1 Gripper Stack Area 2 Exit Stack Area 3 SL Worksurface SL Warm up Advanced SL Warm Up Biomek 2000 6 5 Hardware and Software Installation Guide Chapter 6 Document 609903 Next you will be prompted to clear the worksurface Cancel Next you will be prompted by the Side Loader Warm Up screen 6 6 Biomek 2000 and Software Installation Guide Docume nt 609903 Biomek 2000 Hardware and Software Installation Guide Chapter 6 Document 609903 Side Loader specific functions are listed below HOME amp BACK button When you use very small increments it is hard to tell whether the arm has actually moved or not and in fact the increment may be so small that it may NOT have moved In other cases if the sight is too close to the Target there may be too much friction for the arm to move properly For either of these situations you can use the Home amp Back button to move the arm to the home position then back to the alignment position This provides a more accurate indication of where the current alignment position is To perform small incremental moves raise the sight above the target comp
68. ot you want to overwrite the previous alignment data If you are performing an upgrade which will require re alignment of the system anyway select Yes to reset the alignment values to the defaults If you do not expect to re align the system then select No Note If Yes is selected remember to go to DIAGNOSTICS to reset the alignment values following software installation 17 Specify the serial port on the computer Com 1 that is being used for communication with the workstation Computer Setup Select the serial port that connects to the Workstation m Serial Port Click Next to proceed 18 Specify the ports on the Biomek Workstation used for each of the options and accessories Note that Com 1 is reserved for the computer and Port 6 is reserved for the Wash Unit and or Vacuum Valve Unit if installed If possible leave Port 3 open unused so that it can be used for diagnostic and troubleshooting functions by Beckman Service Personnel Biomek 2000 4 7 Hardware and Software Installation Guide Chapter 4 Document 609903 Workstation Connection x Select the ports on the Workstation to which your accessories are connected Workstation Ports Port 1 Computer Port 2 Heater Cooler Block Port 3 None Port4 Heater Cooler Block SideLoader Ports Plate Reader Left Carousel Port E Right Carousel Other teen 19 The drop down menu al
69. per Stack Area 2 Exit Stack Area 3 SL Worksurface SL Warm up Advanced Stack Areas 4 Check the equipment configuration for that area by selecting the shelf type or device from the drop down menu at stacks A F 6 26 Biomek 2000 Hardware and Software Installation Guide Document 609903 _ m Empty 7 Empty 7 Em mr d NN 5 Identify any equipment which is currently installed on that stack area Click OK when done Biomek 2000 6 27 Hardware and Software Installation Guide Chapter 6 Document 609903 6 Next the Side Loader Align Dialog will appear 7 Place the Target and Holder at each of the positions as prompted by the screen then use the Side Loader Align dialog to move the Sight to the correct position Click Advance when the Sight is in position Refer to Aligning the Stack Areas Using Auto Align for detailed information on sight target alignment Aligning the Side Loader to the Worksurface If you are manually aligning the worksurface use the following procedure 1 Be sure to perform a Side Loader warm up before proceeding 6 28 Biomek 2000 Hardware and Software Installation Guide Document 609903 2 Always perform a Limits of Travel Calibration before aligning the Side Loader worksurface unless you have just done so as part of the Stack Area alignment To do so select Align Side Loader Advanced Limits of Travel Calibration from the
70. probe will pause at each position for 5 10 seconds to allow you to visually examine the alignment settings The probe then returns to the home position BioWorks Diagnostics x Remove the alignment probe from the head Remove the alignment probe and click OK BioWorks Diagnostics Test Complete Click OK to acknowledge the prompt Aligning the Plate Reader Perform Position Calibration before proceeding If you have a Plate Reader installed on the left of the worksurface select Align Worksurface Plate Reader from the Diagnostics module menu bar to align this position 5 26 Biomek 2000 Hardware and Software Installation Guide Document 609903 Make sure that the Plate Reader drawer is open completely and insert a 96 well Beckman EIA plate into the drawer Perform the alignment at wells Al upper left corner and H12 lower right corner on the 96 well plate At each position position the height of the alignment probe so that it just touches the bottom of the well Test the height by gently trying to lift the plate while the probe is touching it Additional information is provided in the Biomek 2000 Plate Reader User s Guide Position Calibration The Position Calibrate command invokes a procedure which compensates for changes in the cable shape which occur during use You should perform a Position Calibration prior to any worksurface alignment and at least once a week to maintain performance Once thi
71. r Refer to the Plate Reader User s Manual from the original manufacturer Molecular Devices Corp for maintenance and service information There are no user serviceable components under the cover Change lamps only with the power off Never touch any of the fiber optic cables or their housing manifold or rotor connections These fibers are extremely delicate and critical to the performance of the Plate Reader Use only the tools described to perform the steps defined in the Plate Reader user s manual Do not touch or loosen any screws or parts other than those specifically designated in the instructions of the Plate Reader user s manual Doing so could cause misalignment and possibly void warranty Never perform any operation on the Plate Reader in an environment where damaging liquids or potentially damaging gases are present Never touch the surfaces of the interference filters or optical lenses DO NOT install the Plate Reader in a stack location that could inhibit access to the Stop Switch of the SL in the event of an emergency Use only locations 1F1 or 2F1 on the SL To prevent fluid from dripping off the microplate when it is in the reading chamber onto any sensitive optical elements and to minimize potential biohazard exposure to other Plate Reader users observe the following precautions When reading microplates that may have fluid on the underside of the microplate damp dry the underside using a dry paper towel or equivale
72. r holder for any labware which can be held by a Labware Holder Figure 10 Installing a Collar ona Collar Holder Biomek 2000 3 5 Hardware and Software Installation Guide Chapter 3 Document 609903 Installing Tool Racks To place a tool rack onto the worksurface insert the locating pins on the bottom of the holder into the corresponding holes in the worksurface with the Front label facing forward See below Push down on the rack until it clicks into place Figure 11 Installing the Tool Rack on the Worksurface You can then install your pipetting tools into the Tool Rack for use in your methods When installing tools in the Tool Rack make sure that the identification label is facing towards the right hand side of the worksurface and that the Front label and arrow are pointing towards the front of the workstation The Gripper Tool rack is installed in the same way with the cut out to the right 3 6 Biomek 2000 Hardware and Software Installation Guide Document 609903 To install the Gripper Tool for use by the Biomek place it in the Biomek Gripper Tool Rack as shown below Figure 12 Installing the Gripper Tool Make sure that the side with the double grip pads is towards the front of the Biomek Note that the Tool Rack is keyed so that the Gripper Tool will only fit in the correct orientation If the tool is not seating properly in the rack turn it around and t
73. rm up Grip Force Limits of Travel Calibration Incubator Advanced Em t When you select this command from the menu the Biomek Side Loader arm moves automatically to set the range of travel The Limits of Travel Calibration Function is a fully automatic routine which moves the Side Loader arm through a range of positions to its maximum and minimum heights extends the arm to its maximum reach flexes the wrist from side to side and then opens and closes the grip This is done to set the maximum positions for these movements 6 32 Biomek 2000 Hardware and Software Installation Guide Document 609903 Incubator This command is used to test the functionality of the push rods and the position of the Incubator To access the Incubator Alignment the Incubator must be configured within a Stack Area dialog as shown below Side Loader Section 1 Stacks Empty Cancel Empty Empty 1 B Incubatoar 4 Incubator Partal For proper operation of the Incubator it is important that the unit is level with respect to the rest of the system Use this command to verify that the Side Loader is able to access each of the positions properly and adjust the level of the Incubator as needed If realignment of the incubator position is needed remove the incubator from the stack area and perform an alignment of the stack area using the target and sight Biomek 2000 6
74. rvoir at the location where the Wash tool will reside Seat the reservoir snugly onto the support block then fit the two locating pins of the support block into the corresponding holes in the tool rack as shown below This reservoir is used to catch the liquid purged from the wash tool as part of the wash tool method Caution Be sure that the reservoir fits snugly in the support block If it is not placed properly on the block the Wash tool may be damaged when the Biomek uses the tool Figure 16 Installing the Wash Tool Reservoir Support Block 3 10 Biomek 2000 Hardware and Software Installation Guide Document 609903 Chapter Installing Bio Works Software Overview BioWorks is the software used to control the Biomek workstation and its devices from the workstation computer The software on the CD ROM must be installed using the BioWorks installation program which is also provided on the CD ROM During the BioWorks software installation process you can specify the tools labware and devices for your system You can also use the BioWorks setup option to add or remove labware tools and devices at a later time During software installation you will be prompted to enter the following information a user name company name and the serial number of the instrument the drive and directory where you want the BioWorks program files to reside whether or not you want to overwrite existing alignment files
75. ry placing it the other way Note Squeeze the fingers of the Gripper Tool together until the arms are parallel to place it into the Tool Rack Installing Tube Racks To place a tube rack adapter plate on the worksurface insert the locating pins on the bottom of the holder into the corresponding holes in the worksurface with the Beckman logo facing forward See below Click into place Then place the tube rack onto the adapter plate with the Beckman logo facing forward Note Tube rack holders may only be placed in a front to back orientation as shown in the illustration below Biomek 2000 3 7 Hardware and Software Installation Guide Chapter 3 Document 609903 Figure 13 Installing Tube Rack Holders on the Worksurface Make sure that this is not placed to the right of a Vacuum Manifold if the Vacuum Manifold is located in the B row as the tube rack will block the vacuum hose from the manifold Installing the 96 Filtration System Select a position for the base which allows access to the vacuum Positions A4 and A5 or A6 if the right side module is installed are the most convenient for routing of the vacuum tubing Orient the base so that the vacuum tubing fitting points towards the back of the Biomek 2000 Note If you have a Side Loader on the system you cannot install the mani fold or any other device in a Side Loader accessible location on the worksurface If you are using a collection pla
76. s eiae epit ie tede 5 10 Worksurtace Alignment iege tdi Lah hte reti die etie Per tree eee rg 5 11 General Notes decedere Dant Wht nels e t nies 5 11 Alignment o Bp P tende a duce Feel e be reden 5 12 Aligning the Worksurface 5 5 nonna eque mde 5 12 Aligning the Base Module 2 d ree ee ete edle ee ree nte acad 5 13 Aligning the Left Side Module esee enne 5 19 Aligning the Right Side Module sse rennen 5 24 Testing th Alignment eet a tae hee aae ie TES 5 24 Aligning th Plate Reader tee reet eee tee 5 26 Position Calibration e Pede 5 27 ij Biomek 2000 Table of Contents Installation and Operations Manual Document 609903 AA Side Loader Alignment G n ral Information 5 eee rete He EC SER ERES TERCER 6 1 General NOES ive derer ri ee e P b dee te eee eoi ee re ete nde 6 1 Alignment Fools ge e E dede core cda iie eed 6 2 Keyboard Support for Alignment eese eene 6 3 Common Command Buttons eee eene nennen rennen nennen enne 6 4 The Side Loader Warm Up Routine eese eene nennen 6 5 Aligning the Stack Areas Using Auto Align eene nennen 6 8 Preliminary Functions uei ite CEPR o e i eee rende br 6 8 The Side Loader Alignment Dialog eere 6 12 Stack Area T H
77. s are decremented for each step that the arm moves down e Wrist values are incremented for each step that the hand pivots in the Right direction Values are decremented for each step that the hand pivots in the Left direction Worksurface Alignment Use the procedure in this section to align the Biomek worksurface General Notes If you are upgrading to BioWorks software version 3 0 from version 2 0 or earlier you must do a full alignment after installing the software For other software upgrades refer to the Release Notes or the ReadMe file which accompany the software Whenever the worksurface is aligned the side module s and or Plate Reader if present as well as the Gripper Tool and Side Loader must subsequently be aligned Alignment of the worksurface overwrites the alignment values for the side modules and the Plate Reader on the left side module Align the worksurface segments in this order e Base module e Left Side Module e Right Side Module If an alignment tip is damaged even slightly by contact with the worksurface replace the tip before proceeding Note Perform Position Calibration prior to any alignment Biomek 2000 5 11 Hardware and Software Installation Guide Chapter 5 Document 609903 Alignment Tools The worksurface alignment probe is shown below pm TA ll S SS Ss E Figure 19 Worksurface Alignment Probe
78. s function begins you can only stop it by pressing the Emergency Stop switch on the worksurface Biomek 2000 5 27 Hardware and Software Installation Guide Chapter 5 Document 609903 5 28 Biomek 2000 Hardware and Software Installation Guide Document 609903 Side Loader Chapter Alignment General Information Use the following procedure to align the Biomek worksurface and stack areas for use with a Side Loader as needed When using the alignment dialog you can use either the mouse to click on the control buttons or you can use the numeric keypad commands General Notes Be sure to perform Side Loader warm up before proceeding Always perform the Limits of Travel Calibration function before performing an alignment of the Side Loader When you use the Auto Alignment option the Limits of Travel Calibration is automatically performed at the beginning of the sequence Check Finger Pads for damage and repair if necessary per Beckman Gripper Pad Installation instructions Publication No 609337 AB Check Side Loader Gripper Arm Alignment with the Arm Alignment tool p n 609809 per instructions given in the Beckman Gripper Arm Alignment document Publication No 609778 AA Be sure to provide good support for rear section of incubator otherwise stack area may get distorted Use leveling feet to adjust tilt and support of incubator unit When using the controls on the Alignment dialog left and righ
79. stem For other upgrades refer to the release notes for the new release Alignment may also be required after installation of new worksurface components such as side modules and any time that an alignment problem is suspected Side Loader You should align the Side Loader after you have done any of the following e Re aligned the worksurface e Replaced the fingers on the Side Loader e Moved or displaced the stack areas of the Side Loader e Replaced any other part of the Side Loader or installed a new Side Loader e Upgraded software from version 2 0 or earlier to version 3 0 In addition you may need to align the Side Loader if you observe errors in labware handling such as dropping lids or difficulty in placing labware in the holder Note Check Side Loader Gripper Arm Alignment with arm Align ment tool p n 609809 per instructions provided in the Beckman Gripper Arm Alignment document Publication No 609778 Gripper You should align the Gripper Tool after you have done any of the following e Installed the Gripper for the first time e Re aligned the worksurface e Replaced the finger pads on the Gripper Tool e Replaced any other part of the Gripper Tool or installed a different Gripper Tool In addition you may need to align the Gripper Tool if you observe errors in labware handling such as dropping lids or difficulty in placing labware in the holder Note Check Side Loader Gripper Arm Alignment with arm
80. t are relative to the operator standing at the front of system facing the tower That is the Left button moves the arm in a clockwise direction through the pie sections from section 2 toward section 1 The Right button moves the arm in a counter clockwise direction around the pie sections from section 1 toward section 2 Biomek 2000 6 1 Hardware and Software Installation Guide Chapter 6 Document 609903 If a collision error occurs during any alignment remove the cause of the collision and repeat the alignment at that position If the sight was knocked out of the Side Loader hand use the Regrip button to regrip the sight in the hand If you are aligning fewer than 3 stack areas do not use the Auto Align program Rather select the appropriate sections manually from the menu The actual alignment prompts and operator actions are virtually the same Note The Auto align program aligns each stack area and worksurface in succession If a problem occurs during this process all previous alignment points could be lost It is recommended to perform align ment on one section at a time to minimize the loss of alignment points Alignment Tools Provided with your Biomek Side Loader are the alignment tools to be used during the alignment process These are the Stack Area Target Holder the Target and the Sight and are illustrated below You will also need an eight shelf stack for teaching shelf height Quarter Circles Used
81. t each position 8 When you get to position 1F1 you will be prompted to place the Target as before If there is no Plate Reader present at 1F1 then place the Target and align as before If you do have a Plate Reader at this position use the Skip button Biomek 2000 6 17 Hardware and Software Installation Guide Chapter 6 Document 609903 9 When you have aligned position 1F1 you will be prompted to place an 8 Shelf Stack at 1A1 Install the stack then click OK when done C _ 10 Use the alignment dialog to align the Sight with the top position of the 8 Shelf Stack The Sight should be flush with the shelf horizontally centered on the shelf side to side and inserted so that the Sight is almost touching the back edge of the shelf 11 When aligned click Advance 12 The final alignment position for the first stack area is the SL Disposal location to the left of position 1A1 13 Click OK to acknowledge the prompt then use the alignment dialog to center the sight over the disposal location Click Advance when you are done This concludes alignment of the first stack area 6 18 Biomek 2000 Hardware and Software Installation Guide Document 609903 Stack Areas 2 and 3 1 After alignment of the first stack area you are prompted to place the Target at location 2A1 00 _ 2 Click to continue or click Skip to skip this alignment position as before 3 Use the same technique to alig
82. taminated where it is appropri ate to do so If any parts are returned they must be enclosed ina sealed plastic bag stating that the contents are safe to handle and are not contaminated e Do not replenish bleach in a sterilization reservoir using the aspirate and bulk dispense transfers as the bleach may damage the Wash tool Accessory Safety Follow the appropriate safety instructions for the accessories listed below in addition to the standard safety precautions for the Biomek 2000 Biomek 2000 ix Safety Side Loader SL Verify that the voltage selector is set for current voltage by a Beckman Representative at the time of installation During method operation DO NOT touch change or otherwise interfere with labware on the worksurface stacks or in the SL hand unless the system tells you to The arm can move suddenly Stay clear of the arm and hand during operation Keep the area around the SL clear of any obstructions to avoid collisions There is a Stop switch at the base of each Stack Area If an emergency stop is required pressing this on each stack area of your SL switch will freeze the SL instantly CAUTION The Stop switch on the SL will not stop the workstation nor will the Stop switch on the workstation stop the SL NOTE You may lose data and be forced to quit the run in progress if you press the SL Stop switch Use of the SL stop switch may result in spill age or splattering of liquids in transit
83. te place it into the base Make sure that it is centered Place either the Microplate Collar or the Square Well Plate Collar on the base as needed Orient the collar so that it is centered on the base and so that the pins slip into place Place the filter plate on top of the collar Note Before each use make sure that the sealing surfaces of the manifold are clean and free of any chemical residue If there is any residue present the pieces may not seal properly Handle the collars carefully to avoid nicks and scratches which could affect the seals 3 8 Biomek 2000 Hardware and Software Installation Guide Document 609903 In the following illustration the Square Well Plate Collar is used with a Square Well collection plate Figure 14 Installing a Manifold Square Well Plate Collar and Plates on the Worksurface The following illustration shows use of the Microplate Collar for wash operations In this arrangement the filtrate is aspirated to a waste collection flask The Microplate Collar can also be used to collect filtrate to microplates soose coa Figure 15 Installing a Manifold Microplate Collar and Plate on the Worksurface Biomek 2000 3 9 Hardware and Software Installation Guide Chapter 3 Document 609903 Installing the Support Block for the Wash Tool Reservoir If you use a Wash tool in the system configuration be sure to place the support block provided under the 1 4 rese
84. tion Guide Document 609903 4 The Stack Area configuration windows will open for each SL section Configure each stack area in a section to empty when performing alignment Stack Area F in Sections 1 and 2 may be configured for a Plate Reader Biomek 2000 6 9 Hardware and Software Installation Guide Chapter 6 Document 609903 5 Each area must be designated as Empty for alignment purposes Click OK to proceed 6 10 Biomek 2000 Hardware and Software Installation Guide Document 609903 6 Each area must be designated as Empty for alignment purposes Click OK to proceed 7 All stack area configurations should be designated on these screens Side Loader Alignment should be performed with Stack areas empty For each stack location A through F for sections 1 amp 2 and A through D for section 3 select the type of shelf or device present if any from drop down menu shown above This information is used during SL Warm Up so that the arm does not collide with any of the equipment at the stack locations 90 Biomek 2000 6 11 Hardware and Software Installation Guide Chapter 6 Document 609903 10 To obtain 100 alignment the Side Loader warm up routine should be run prior to aligning the Side Loader 11 When you have completed the configuration select OK The Side Loader Alignment Dialog For a discussion of the Movement Increment and Table of Values fi
85. u work for and the product serial number Name Company Serial Cancel 10 Next you are asked to enter the directory name for installation of the BioWorks files You can either accept the default name shown or you can enter a new directory name Choose Destination Location Setup will install Bio Works in the following folder To install to this folder click Next To install to a different folder click Browse and select another folder You can choose not to install BioWorks by clicking Cancel to exit Setup Browse button Destination Folder C BIOWORKS Browse lt Back Cancel 11 Click the Browse button to prompt the Choose Folder screen 4 4 Biomek 2000 Hardware and Software Installation Guide Document 609903 Choose Folder x Please choose the folder for installation Path C BIOWORKS OK Directories E c BIOWORKS B00K1 C3 DDETest 3 FLOPPY C INITDB Drives grosse Ff 12 Click OK to proceed Note Do not select the Network button Bio Works is not supported when installed on a network drive Lab Book Placement xi The setup program will place a Lab Book database into the following directory path and assign a name to it Any existing lab books will be saved and imported to the new database Lab Book Path EABIOWORKS B00K1 Browse Book Name
86. umber 609242 to Port 1 on the workstation as shown below Connect the other end of the cable to the serial port on the computer 22 Figure 1 Connecting the Computer to the Biomek Workstation Biomek 2000 Hardware and Software Installation Guide Document 609903 3 Ifyou are installing a Wash Unit or a Vacuum Valve Unit connect it to Port 6 of the Biomek workstation If you are installing both connect them in series and install the terminator plug on the last unit in the chain 4 Other accessories such as the Plate Reader or Heater Cooler Block controller may be connected to the remaining ports on the Biomek workstation Caution Avoid using Port 3 if possible This port is used by Beckman Field Service personnel during maintenance and troubleshooting Plate Reader HCB Controller Wash Unit Port 6 Figure 2 Connecting the Wash Unit Plate Reader and Heater Cooler Block 5 If you are installing a Side Loader connect the Side Loader Control Module to one of the remaining ports on the Biomek workstation Avoid using Port 3 if possible See Figure 3 Note When installing options and accessories refer to the instructions which were provided with each of the accessories for complete instal lation instructions specific to each item Biomek 2000 2 3 Hardware and Software Installation Guide Docu

Download Pdf Manuals

Related Search

Related Contents

ICC ICACSWRCTR  EPSON LX-300+ Service Manual  FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α Clone  3G Remote Alarm Camera user manual  Guía del usuario Medidor doble de humedad Pro  Full Potential Local Orbital Minimum Basis Bandstructure Scheme  Altec Lansing 605B User's Manual  Sony DSC-P3 Operating Instructions  Nikon SB-N5 User's Manual  取扱説明書  

Copyright © All rights reserved. Failed to retrieve file