Assay-specific Protocol Instructions
Contents
1. When working in a laboratory use personal protective equipment including lab coat disposable gloves and protective eyewear For Technical Support amp Additional Information Please refer to the Novallele Genotyping Assay User Manual for support and additional terms amp conditions Visit www canon biomedical com Call 1 844 CANON BIO Canon BioMedical Inc One Canon Park Melville NY 11747 Novallele is a trademark of Canon BioMedical Inc 2015 Canon BioMedical Inc All Rights Reserved 50089 Rev 0 Last revised 5 2015 Visit www canon biomedical com Call 1 844 CANON BIO Canon BioMedical Inc One Canon Park Melville NY 11747 Novallele is a trademark of Canon BioMedical Inc 2015 Canon BioMedical Inc All Rights Reserved 50089 Rev 0 Last revised 5 20152. Canon BioMedical Inc BTD c 1330G gt C Novallele Genotyping Assay Product Specification Sheet Catalog Number 40045 Reagent ID z Stable for six 6 months after receipt For Research Use Only Not for use in diagnostic procedures Canon BioMedical Inc BTD c 1330G gt C Novallele Genotyping Assay Product Specification Sheet Catalog Number 40045 Reagent ID 7 Stable for six 6 months after receipt For Research Use Only Not for use in diagnostic procedures Introduction Novallele Genotyping Assays identify the allelic composition of a locus using fast PCR by high resolution melting HRM This one 1 vial of 600 uL Novallele Genotyping Assay contains all of the primers probes required Storage amp Shelf Life Novallele Genotyping Assay should be stored at 2 8 C When stored correctly this product is stable for six months from the date of receipt Introduction Novallele Genotyping Assays identify the allelic composition of a locus using fast PCR by high resolution melting HRM This one 1 vial of 600 uL Novallele Genotyping Assay contains all of the primers probes required Storage amp Shelf Life Novallele Genotyping Assay should be stored at 2 8 C When stored correctly this product is stable for six months from the date of receipt General Use Instructions This protocol is for use with Novallele Genotyping Assays and reagents on non Canon BioMedical thermocy
3. R plate disc 6 Remove any air bubbles in any of the plate wells by centrifugation 7 Program the thermocycler according to the recommended conditions below Hot Start Cyclex Temp Time Ramp Rate 1 x 95 C 00 30 4 4 PCR Cyclex Temp Time Ramp Rate 35 x 95 C 00 02 4 4 70 C 00 08 2 2 7 Program the thermocycler according to the recommended conditions below Hot Start Cyclex Temp Time Ramp Rate 1 x 95 C 00 30 4 4 PCR Cyclex Temp Time Ramp Rate 35 x 95 C 00 02 4 4 70 C 00 08 2 2 Pre Melt Cyclex Temp Time Ramp Rate 1 x 94 C 00 05 4 4 40 C 00 03 2 2 HRM Temp Time Ramp Rate 50 C 00 05 2 2 95 C at 0 3 C 5s 8 Place the PCR plate disc in the thermocycler Start the run Pre Melt Cyclex Temp Time Ramp Rate 1 x 94 C 00 05 4 4 40 C 00 03 2 2 HRM Temp Time Ramp Rate 50 C 00 05 2 2 95 C at 0 3 C 5s 8 Place the PCR plate disc in the thermocycler Start the run Safety Information Please follow your local institutional safety policies and practices When working in a laboratory use personal protective equipment including lab coat disposable gloves and protective eyewear For Technical Support amp Additional Information Please refer to the Novallele Genotyping Assay User Manual for support and additional terms amp conditions Safety Information Please follow your local institutional safety policies and practices
4. clers Please do not substitute other reagents For additional information please refer to the Novallele Genotyping Assay User manual Procedure 1 Briefly invert all of the Novallele reagents to mix the contents of each tube 2 Briefly centrifuge all of the Novallele reagents 10 15s 3 Prepare a reaction mix for each sample General Use Instructions This protocol is for use with Novallele Genotyping Assays and reagents on non Canon BioMedical thermocyclers Please do not substitute other reagents For additional information please refer to the Novallele Genotyping Assay User manual Procedure 1 Briefly invert all of the Novallele reagents to mix the contents of each tube 2 Briefly centrifuge all of the Novallele reagents 10 15s 3 Prepare a reaction mix for each sample For technical replicates in triplicate Sample DNA 220ng Nuclease free water X uL Novallele Genotyping 11 uL Mastermix Novallele Genotyping Assay 11 uL Mix Total volume 33 uL 4 Add 10 uL reaction mix to each well 5 Tightly seal the PCR plate disc 6 Remove any air bubbles in any of the plate wells by centrifugation For technical replicates in triplicate Sample DNA 220ng Nuclease free water X uL Novallele Genotyping 11 uL Mastermix Novallele Genotyping Assay 11 uL Mix Total volume 33 uL 4 Add 10 uL reaction mix to each well 5 Tightly seal the PC
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