Out-of-Compartment Microplate Reader
Contents
1. 01 Page 7 PIIKE TECHNOLOGIES Spectroscopic Creatit ity 6125 Cottonwood Drive Madison WI 53719 5120 608 274 2721 TEL 608 274 0103 FAX info piketech com www piketech com2. 53719 Printed in the United States of America All world rights reserved No part of this publication may be stored in a retrieval system transmitted or reproduced in any way including but not limited to photocopy photograph magnetic or other record without the prior written permission of PIKE Technologies Inc Address Comments to PIKE Technologies Inc 6125 Cottonwood Drive Madison WI 53719 Phone 608 274 2721 Fax 608 274 0103 E mail info piketech com Web Site www piketech com Jan 1 2014 Contents Introduction Specifications Unpacking Your Accessory Packing List Optical Description Installation PC and Power Connection Connecting to your Computer Powering Up the Accessory Accessory Initialization NID ID I D IR WIN IN RIB Introduction The Out of Compartment Microplate Reader offers high throughput plate reading capability to UV Vis spectrophotometers with standard sample compartments This allows flexibility to researchers conducting experiments with traditional accessories such as cuvettes temperature control and integrating soheres who may also require microplate reading functionality Microplate reading capability is often required in research drug discovery bioassay validation quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academia The accessory supports standard 96 well and 384 well formats The Microplate Reader can also be adapted to perfo
3. Installation and User Guide TECHNOLOGIES Spectroscopic Creativity l I PIKE Out of Compartment Microplate Reader The information in this publication is provided for reference only All information contained in this publication is believed to be correct and complete PIKE Technologies Inc shall not be liable for errors contained herein nor for incidental or consequential damages in connection with the furnishing performance or use of this material All product specifications as well as the information contained in this publication are subject to change without notice This publication may contain or reference information and products protected by copyrights or patents and does not convey any license under the patent rights of PIKE Technologies Inc nor the rights of others PIKE Technologies Inc does not assume any liability arising out of any infringements of patents or other rights of third parties This document contains confidential or proprietary information of PIKE Technologies Inc Neither this document nor the information herein is to be reproduced distributed used or disclosed either in whole or in part except as specifically authorized by PIKE Technologies Inc PIKE Technologies Inc makes no warranty of any kind with regard to this material including but not limited to the implied warranties of merchantability and fitness for a particular purpose Copyright 1991 2014 by PIKE Technologies Inc Madison WI
4. mpartment c Slide the detector cable grommet in the Detector Cable Access Plate slot and re attach the plate to the back of the instrument PN 350 047100 01 Page 4 Detector Cable Access Plate Grommet 3 Attach the fiber optic probe to the SMA connector marked Light Out on the Transfer Optics Module de Agilent Techno logia Gory Uy te Figure 2 Completed System 4 Complete the PC and PC connection section on the next page 5 Slide the probe into the probe holder located on the top of the accessory Enable the align function in the spectrophotometer software to monitor the energy Adjust the probe up and down until the position of maximum energy is determined Secure the probe by tightening the two set screws of the probe holder Probe Holder Set Screws a i PN 350 047100 01 Page 5 PC and Power Connection Before performing these steps please ensure that the spectrophotometer power is off Power pawl Terminal On Off HAS DCOutFuse AN USB A 250VTL l l i Connector Fuse Figure 3 Connector panel located on the back panel of the Microplate Reader Connecting to your Computer Please load the AutoPRO6 software before connecting the accessory to the PC Instructions for the AutoPRO6 software are covered in a separate manual Locate the USB port on top of the accessory panel Connect the USB cable to that port and to a USB port on your computer Powering Up the Access
5. ory Plug the DC power line connector to the matching DC power jack on the accessory panel Connect the power supply to an AC wall outlet The green power on line LED light will turn on Please refer to a separate manual for AutoPRO6 Software installation and use PN 350 047100 01 Page 6 Accessory Initialization 1 Set up AutoPRO6 software for the correct accessory gt mm degrees X Point Definition 96 Point Point Shape Circle Point Width 6 mm Point Height 6 mm Offset x y O O mm Well Spacing x y 9 9 mi Spectra Files o O amp S k k 2 Setthe accessory to manual mode ee B AutoPRO Default yy File Edit View Units Hardware Help A Points Point Definition 96 Point Point Shape Circle Point Width 6 mm Point Height 6 mm Offset x y 0 0 mm Well Spacing x y 9 9 m 3 Spectral Files 3 Click the Initialize Motors icon the stage will move slightly and reposition itself at position 0 0 AutoPRO Default SON N Eile Edit View Units Hardware Help Point Definition 96 Point Point Shape Circle Point Width 6 mm Point Height 6 mm Offset x y O 0 mm Well Spacing x y 9 9 m 7 Spectral Files After this step the Microplate Reader is ready for use and you can set up your experiment as described in the AutoPRO6 User Manual PN 350 047100
6. rm automated measurements of filters optical components and other materials Specifications Transfer Optics Module and two UV Vis NIR Optical Fiber Cables or Transfer Optics Module one Optical Fiber Cable and Photodiode Detector Mechanical Specifications Repeatability 5 um Resolution 1um Run Time 96 well plate 32 seconds 384 well plate 84 seconds The Microplate Reader is controlled by PIKE Technologies AutoPRO 6 software which can be integrated with most commercial FTIR and NIR software packages Launch optics fiber coupler fitted into the spectrophotometer is required PN 350 047100 01 Page 1 Unpacking Your Accessory In order for you to quickly verify receipt of your accessory we have included a packing list Please inspect the package carefully Contact PIKE Technologies if any discrepancies are noticed Packing List User Manual AutoPRO6 Manual and Software Microplate Reader Accessory PN 350 0047000 PN 350 000071 PN 047 10XXX Quantity 1 Quantity 1 Quantity 1 UV Vis Fiber Optical Probe USB Cable Cary 60 Detector Cable Access 1 m Probe Sold Separately Plate PN 047 3030 Quantity 1 Quantity 1 Power Supply with Power Line Cord Quantity 1 PN 350 047100 01 Page 2 Optical Description The optical system of the Microplate Reader features a fiber optic probe and a built in silicon diode detector The light is picked up in the spectrophotometer sample compartment by the Tran
7. sfer Optics Module and sent to the plate reader via the optical fiber probe After passing through the sample it is collected by the detector and registered by the spectrophotometer electronics Optical Beam Path Sampling Plate Figure 1 Optical cutaway view The mechanical design of the accessory relies on an X Y stage with both axes driven by high precision servo motors with optical encoders for speed and reproducibility USB and DC power are the only external connections required for this accessory Programming and control of the Microplate Reader is done through PIKE Technologies AutoPRO6 software which can be integrated easily with the spectrophotometer software PN 350 047100 01 Page 3 Installation The Microplate Reader module features a small footprint and can be positioned next to or above the spectrophotometer Before performing these steps please ensure that the spectrophotometer power is off 1 Mount the Transfer Optics Module in the sample compartment of the spectrophotometer Follow manufacturer s instructions to complete this step 2 Position the Microplate Reader in a desired location and plug the detector cable into the socket located in the sample compartment of the spectrophotometer as follows a Remove the access plate from the spectrophotometer back wall b Feed the detector cable through the opening and plug it into the socket located on the side wall of the spectrophotometer sample co
Download Pdf Manuals
Related Search