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ImageQuant™ LAS 4000 mini - GE Healthcare Life Sciences

1. Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 9 1 Introduction 1 3 The ImageQuant LAS 4000 mini Part Name Description 7 Sample tray Epi tray for chemiluminescence and Epi lights UV trans tray for UV transilluminator optional NP tray for titer plates 8 Manual filter changer For inserting filters 9 UV expansion bay For UV transilluminator optional 10 Power switch Turn the ImageQuant LAS 4000 mini on or off Computer The ImageQuant LAS 4000 mini is operated using a PC running Windows XP or VISTA It is not designed for standalone operation Note The computer used should be certified according to IEC UL 60950 1 4 ImageQuant LAS 4000 mini Control Software Exposure and exposure settings are controlled by the ImageQuant LAS 4000 mini Control Software The software user interface is described below ImageQuant LAS 4000 1 Fie ER View Opon Hep ImageQuant AS 4000 mini 2 Exposure Type Exposure Time Aio 3 C Mansal 4 Sensitivity Resolution 5 6 10 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 1 Introduction 1 4 ImageQuant LAS 4000 mini Control Software Part Name Description 1 Menu bar Access to program functions 2 Exposure Type Set the exposure method 3 Exposure Time Set the exposure time 4 Sensitivity Resolution Set the sensitivity and resolution 5 Add Digitization Image Adda white light exposure to
2. 6 Troubleshooting 6 1 Problems with the exposed image 6 1 Problems with the exposed image Phenomenon An appropriate image does not appear Cause Image is overexposed Countermeasure Shorten the exposure time or check the iris setting Image is underex posed Make the exposure time longer or check the iris setting The focus is not cor rectly adjusted Adjust the focus correctly The tray lens or filter are dirty Clean the tray lens and filter See Chap ter 5 Maintenance on page 53 The wrong filter is in place Install the correct filter The wrong lens is in stalled Install the correct lens The wrong sample tray is being used Change to the correct sample tray The wrong light source is selected Select the correct light source The Flat Frame file is not exposed correctly Expose the Flat Frame again The size of the object to be exposed does not coincide with the exposed area Place the sample correctly on the sample tray and place the tray at the correct tray position Light leaks on the image The ImageQuant LAS 4000 mini door is not closed completely Close the ImageQuant LAS 4000 mini door and expose the image again Exposure to direct sunlight Avoid direct sunlight The image appears misty Dew condensation in the optical system Quit the ImageQuant LAS 4000 mini Con trol Software and wait until the operation env
3. Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 37 3 Installation 3 5 Installation of hardware 3 5 5 Installing Epi light sources 2 Put the Epi light source hanger on the guide Then slide the light source inwards to attach it This will connect the light to the power connector 3 Fix the Epi light source into position using screw A Epi light source Screw A FS Note The Epi lights are clearly labelled L for the left and R for the right sides e install the Epi lights in matching pairs only If Epi lights with different wavelengths are used the intended image will not be obtained 38 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 4 Operation 4 Operation This chapter describes how to operate the ImageQuant LAS 4000 mini and how samples are to be placed in the instrument The steps required to record an image are also described 4 1 Operation overview The general flow of operation is as follows Turn on the instrument and computer Preparation Start the Control Software Place the sample Exposure Expose the sample Analyze the image 4 2 Preparation 4 2 1 Starting the ImageQuant LAS 4000 mini and Control Software The ImageQuant LAS 4000 mini and Control Software are started as follows Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 39 4 Operation 4 2 Preparation 4 2 1 Starting the ImageQuant LAS 4000 mini and Control Software 1
4. The lens is not correctly in stalled Install the lens correctly The iris is not correct Check the iris value The image is noticeably out of focus Adjust the focus Even when the set exposure time has elapsed the reading operation is not completed The ImageQuant LAS 4000 mini power has been turned off during exposure Turn the power switch of the ImageQuant LAS 4000 mini on and reboot it After exposure no image is displayed on the monitor The exposure time is too short Make the exposure time longer The light source is not correct Install the correct light source The filter is not correct Install the correct filter The sample is not in position Check the sample position The focus is not correct Adjust the focus Exposure cannot be performed even if the Start button has been pressed USB cable connection failure Connect the USB cable correctly The LAS High Sens lens is not recog nized Lens cable connection failure Connect the lens cable correctly The light source is not recognized The light source is not con nected correctly Connect the light source correctly No button is displayed in Focusing mode Lens cable connection failure Connect the lens cable correctly 6 5 Message ImageQuant LAS 4000 hardware error occurred SensKey H SensCode H is displayed If the error persists even after the following
5. gt gt E gt B gt gt Be D Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 15 2 Safety instructions 2 1 Safety precautions CAUTION Do not repeatedly turn the power switch on and off unnecessarily This may cause malfunction CAUTION Do not leave samples in the equipment after exposure If left these may degrade and cause damage CAUTION The Epi lights are in sets of two for the left and right sides Ensure that they are correctly installed Do not mix light sources of differing types CAUTION Ensure that the Epi lights are connected and locked in position before turning the power on CAUTION Keep the windows of the light sources clean and avoid touching them NOTICE The UV trans tray will deteriorate with repeated UV radiation This is normal Replace the UV trans tray when this deterioration becomes noticable in exposed images 00D P PP Pe NOTICE Do not disconnect the USB cable while the Control Software is running Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 2 Safety instructions 2 1 Safety precautions Maintenance WARNING Do not remove the covers of the camera head or the ImageQuant LAS 4000 mini High temperature and high voltage parts could result in burns or electric shock Contact your local GE Healthcare representative to request internal inspection servicing and repairs
6. ring INformations nad m pt MU FRE 74 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 1 Introduction 1 Introduction Purpose of this manual Getting Started with ImageQuant LAS 4000 mini provides instructions needed to handle the ImageQuant LAS 4000 mini in a safe way Basic operating instructions are also provided For details refer to the ImageQuant LAS 4000 mini User Manual Prerequisites In order to operate the ImageQuant LAS 4000 mini in the way it is intended the following prerequisites must be fulfilled e You should have a general understanding of how a PC and Windows work You must read the safety instructions outlined in the user documentation You should be acquainted with the use of general laboratory equipment and with handling of biological materials In this chapter This chapter contains important user information a general description of the ImageQuant LAS 4000 mini and its intended use 1 1 Important user information Read this before using the ImageQuant LAS 4000 al All users must read the safety instructions in the ImageQuant LAS 4000 mini user documentation before installing using or maintaining the equipment Do not operate the ImageQuant LAS 4000 mini in any other way than described in the user documentation Otherwise you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment Getting Started with ImageQuant LAS 4000
7. 4 5 Performing an exposure 4 3 5 Set exposure sensitivity Sensitivity No pixels binned WxH No pixels final image W x H Super Binning 384 x 256 384 x 256 Ultra Binning 192 x 128 192 x 128 Step Action 1 Select Sensitivity from the drop down list Sensitivity Resolution High Resolution High Binning Super Binning Eo Ultra Binning rc Note TIP 4 3 6 Exposure Step The Add Digitization Image function can be used for chemiluminescent samples This creates a composite image of a chemiluminescence exposure and a digitized white light exposure in order to add an image of an opaque molecular marker with a single click Select Sensitivity Resolution in the Help menu This displays a diagram that describes the relation between sensitivity and resolution Action 1 Click the Start button to begin exposure Note The orange Busy LED lights on the ImageQuant LAS 4000 mini during exposure Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 49 4 Operation 4 3 Performing an exposure 4 3 7 Save the image 4 3 7 Save the image Once the sample has been exposed the image will be loaded into the main window Step Action 1 Adjust the saturation and contrast of the image using the controls at the bottom of the window Part Function 1 Adjust the dynamic range by dragging the limits with the mouse 2 The gradation conve
8. No CCD calibration file ex ists Create a correction file using the CCD Calibration function in the Option menu Perform calibration for both over 2 hours and from 1 100 sec to 2 hours NP Tray can only be used in tray po sition 3 with the FUJINON VRF43LMD lens and the chemiluminescence method NP tray is not in the proper position The NP tray can be used only with the LAS High Sens lens and at tray posi tion 3 Place the NP tray at tray posi tion 3 Use the LAS High Sens lens Please set the tray so that contact is made with the sensor at the rear of the cabinet The tray is not detected Insert the tray and ensure it is pushed fully in Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 6 Troubleshooting 6 6 Error messages and countermeasures Start button during exposure Error message Meaning Countermeasure Cannot continue with the automatic The automatic exposure Change the settings appropri exposure function The sample may function is unable to calcu ately or perform an exposure be out of the photometric range or late a correct exposure in Manual mode a setting may need to be adjusted time Exposure has been stopped The UV transilluminator Reduce the total exposure time The UV illuminator was turned off was turned off so that the UV transilluminator because the 10 minute limit was is lit for less than 10 minutes reached Attention T
9. Ultraviolet rays lamps contain mercury wmm Dispose of properly UV glass filter gt gt S e Cal plates ES CAUTION 4 co amp gtTicUChv4 amp ty Uc RIO ZAMEBISGNY TT MIOTOROC k EBL TRALT GS A 3 JST IMG PIRA ERO odio d I RRL T meu 1 Set this place on the tray with this surface facing down 2 Make sure to confirm that there is no dust or dirt on the top surface opposite of this surface prior to use 3 When this plate becomes durty wash it with neutral detergent take care not to scratch it 22 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 2 Safety instructions 2 2 Labels Sample trays The Epi tray is shown here The White trans tray and UV trans tray are labelled correspondingly Edi FUJIFILM e Epi tray Filters The 605DF40 filter is shown here Other filters are labelled according to their type eS 605DF40 605 EtBr filter FUJIFILM Filter cover 2 5 Emergency procedure In an emergency situation Turn off the ImageQuant LAS 4000 mini power switch e Disconnect the power cable from the wall socket WARNING Access to power switch and power cord Do not block the rear and side panel of the instrument The Power switch must always be easy to access The power cord must always be easy to disconnect Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 23 2
10. WARNING Do not attempt to modify the equipment or fire and electric shock could result CAUTION Take care when connecting the power supply cable Do not tug on the cable and do not handle the connection plugs with wet hands CAUTION Connect the computer hardware on the same power circuit otherwise the equipment may be influenced by electrical nosie CAUTION Turn off the power switch and remove connecting cables before moving the equipment CAUTION Turn the power switch off before cleaning the inside of the equipment CAUTION Unplug the equipment if it will not be used for an extended period gt gt me b Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 17 2 Safety instructions 2 2 Labels 2 2 Labels On the exterior of the ImageQuant LAS 4000 mini there are a number of labels describing equipment specifications as well as precautions necessary to safely use the ImageQuant LAS 4000 mini This section lists the symbols used on the safety labels and shows the location of labels on each component The names of parts on the components are also shown ImageQuant LAS 4000 mini serial number The ImageQuant LAS 4000 mini serial number is located on a label on the back of the instrument 36 GE Healthcare Bio Sciences AB EE d LUMINESCENT IMAGE ANALYZER Model ImageQuant LAS 4000 mini Serial No 123456 Frequency 50 60 Hz Supply Voltage 100 240 V c us
11. objects to get into the camera head or the ImageQuant LAS 4000 mini This may result in fire or electric shock Turn off the power switch unplug the equipment from the power outlet then contact you local GE Healthcare representative WARNING Do not drop or otherwise damage the camera head or ImageQuant LAS 4000 mini This may result in fire or electric shock Turn off the power switch unplug the equipment from the power outlet then contact you local GE Healthcare representative 12 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 2 Safety instructions 2 1 Safety precautions WARNING Do not damage the power supply cord by bending twisting heating or allowing them to become pinned under the equipment Using damaged power cords could result in fire or electric shock gt If the power supply cords are damaged contact your local GE Healthcare representative for replacements WARNING Do not place the equipment on unstable tables or on inclined surfaces as the equipment could be dropped or fall resulting in injury CAUTION Do not place the equipment or parts in direct sunlight This may degrade performance or result in overheating and fire CAUTION Do not scratch or drop parts containing glass such as lenses filters or lights gt bP gt Personal protection WARNING Parts of this equipment emit UV radiation Avoid exposure Wear protective clot
12. office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com quantitative imaging imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company ImageQuant is a trademark of GE Healthcare companies All third party trademarks are the property of their respective owners 2009 General Electric Company All rights reserved First published Oct 2009 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 28 9607 61 AB 11 2009
13. the terms in the license agreement and click the Next button 28 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 3 Installation 3 4 Installation of the software 3 4 1 Installation Windows XP 5 Select destination folder in the dialog ie ImageQuant LAS 4000 InstallShield Wizard Destination Folder Click Next to install to this Folder or click Change to install to a different folder gt Install ImageQuant LAS 4000 to C Program Files Click the Next button to install the software at the default folder C Program Files Click the Change button to install to a different folder 6 Click the Install button 7 Clickthe Finish button The installation of ImageQuant LAS 4000 mini Control Software is now completed 3 4 2 Installation Windows Vista Installation is performed in the following sequence 1 Installation of FUJI USB Control driver 2 Installation of FUJI USB Function driver 3 Installation of ImageQuant LAS 4000 mini Control Software 3 4 2 1 Installation of the USB Control Driver Windows VISTA Note The computer and ImageQuant LAS 4000 mini must not be connected with a USB cable during the operation Note During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation 1 Openthe control panel and click Classic V
14. to 70 C Placement Stable laboratory bench load capacity gt 100kg m At least 20 cm free on all sides Humidity 3096 to 7096 non condensing Storage 596 to 100 non condensing 3 2 Transport The ImageQuant LAS 4000 mini weighs approximately 31 kg and it is recommended that at least two people lift and move it Before moving the instrument 1 Turn off the instrument 2 Disconnect the power cord 3 Remove the lens transmitted light source and any loose parts from inside of the IDX Refer to the ImageQuant LAS 4000 mini User Manual 3 3 Unpacking When unpacking e Check the equipment for any apparent damage before installing Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 25 3 Installation 3 5 Unpacking Document any damage carefully and contact your GE Healthcare representative 5 4 Installation of the software 3 4 1 Installation Windows XP Installation is performed in the following sequence 1 Installation of USB Control driver 2 Installation of USB Function driver 3 Installation of ImageQuant LAS 4000 3 4 1 1 Installation of the USB Control Driver Windows XP Note The computer and ImageQuant LAS 4000 mini must not be connected with a USB cable during the operation Open the control panel and select Printers and Other Hardware Click Add Hardware Click the Next button in the Add Hardware Wizard Select Yes have already connected the hardware and click the Next bu
15. Cat apparel numerique de la classe A respecte toutes les exigences steriel boulleur du Canada t appar jus du Reglement sur le ma Camera head Camera Head 0 Lens mount Mechanical shutter Bottom Connector for IDX Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 19 2 Safety instructions 2 2 Labels LAS High Sens lens F0 85 43mm LAS High Sens Lens SN FUJIFILM y Label Back side End cap Mount Lens control connector Lens cap White Epi light The white Epi light is installed on the left side only K Illumination LED 4 7 Guide pin White EPI for 4000mini SN FUJIFILM Retaining screw hole N 1 position Connector 20 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 2 Safety instructions 2 2 Labels Epi lights The following diagram shows the UV Epi lights Other Epi light pairs are labelled corresponding to their type Guide pin Optical filter and LED Retaining screw hole 1 position Connector i UV Epi light FUJIFILM UV expansion bay UV expansion bay Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 21 2 Safety instructions 2 2 Labels UV transilluminator UV transilluminator oF FUJIFILM Power supply connector Mercury containing Products Label
16. For NP tray The NP tray is to be used at tray position 3 4 Open the IDX door and insert the tray Place the Epi or Trans trays in position with the side with a hole facing outwards 5 Ensure the lens cap is removed and close the IDX door 4 5 Performing an exposure This section describes the steps for exposing a sample and saving the image In this description the screen images shown are for an exposure of a chemiluminescent sample The general procedure is however similar for all types of sample For detailed instructions refer to the ImageQuant LAS 4000 mini User Manual 44 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 4 Operation 4 3 Performing an exposure 4 3 1 Workflow Images may also be obtained by repeated incremental or programmed exposure of a sample For detailed instructions on these types of exposure refer to the ImageQuant LAS 4000 mini User Manual 4 3 1 Workflow The workflow for exposing a sample and recording an image is as follows Choose method and tray position Adjust the focus Set exposure sensitivity Save the image 4 3 2 Choose method and tray position Step Action Click the Method Tray position button Result The Method Tray position dialog opens Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 45 4 Operation 4 3 Performing an exposure 4 3 2 Choose method and tray position Step Action 2 Select a Method for exposure Meth
17. GE Healthcare Getting Started with ImageQuant LAS 4000 mini Original instructions Table of Contents Table of Contents ME reco RmR 5 Li Important user InfanmitiOD caca esda tae are UEBER EISE FI EPASEYEeHE RU E ES UTE ResH S per ec UR EN 5 Lz Regulatory INCODTIDUGTGS osi ad GUI OURUICOU GROS CU OU EID er Genet OU R R ON ONU 7 LS The ImageQuant LAS 4000 Mini aususdisi test RR RI apr tam In Uem man mE UN EIU mem 8 l4 ImageQuant LAS 4000 mini Control Software ccesssssseseesesssssnssssseeseessetsvessssnaseavenssensbessessveessennsussiesuaeveeneeaseespasis 10 E zs Asie ILC eR 12 Pd SUNSDCEPECUKINOR cesses timent ca MEME 12 gus VLADE S ea e a IEEE IRE UNS RE ene rere eeee rrr 18 23 Emergency LO EUIS sninen E N EN 23 24 dRecuclhp MION OtI N azcassceserSazazassvacteverngosnsassssarsenvisaenavasazascesovevsssvotsestaneegastasnasasasinsesesonsssanevanssecsnsusssanenuscesreesnuennitieeenat 24 S Installati n ere ee ER pen Bn rn oe e E E a E E 25 ah SE TOQBFEPTTBEIUS cima n OPFTHERDT EUR EERISHERHU BIN UU TURIS IUS EU LEEHOR D GU ESEESE MEUM 25 EMI 25 22 UDDIBOE BIB scouir oo eb peU e UD NUT ana RU OD ERU HERO DU TT OP reer pene IN PUO RR RE nL MURUS 25 3 4 Installation of the software uisum mtt tinta e autera t Utd Rd us mST eee 26 341 IC o6 Windows XP Pr 26 3 4 2 Installation Windows Vista P ARE 29 35 Installation ot NArIWEre sc
18. Max Current 3 0A LSA M Protection Class 1P20 kuii ai Manufacturing Year MENT NS C m 32406 ewaavw FUJIFILM Corporation TOKY MADE IN JAPAN 405N101507 Symbols used in safety labels Label Meaning Warning Read the user documentation before using the system Do not open any N covers or replace parts unless specifically stated in the user documentation The system complies with the requirements for electromagnetic compliance EMC in Australia and New Zealand C The system complies with applicable European directives Labels concerning use of hazardous substances Label Meaning This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment 18 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 2 Safety instructions 2 2 Labels Meaning This symbol indicates that the product contains hazardous materials in excess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concen 10 tration Limits for Certain Hazardous Substances in Electronics Intelligent dark box IDX This Cass A digital apparatus meets al requirements of the Canadian interference Causing Equipment Reguiations
19. My Document My Computer Fle name i 3 MyNetmok Flee of bree Cornel 11 Open the USB Control folder Looky 2 ImageQuani LAS 8000 D 1 o1 gt m DE My Recert Documenis Dedtop My Document My Computer Fie name at J MyNetreck Fen of ee Cre odo My Documents My Computer Fie pane m3 S 2 MyNetmok Fiss of boe Corel Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 3 Installation 3 4 Installation of the software 3 4 1 Installation Windows XP 27 3 Installation 3 4 Installation of the software 3 4 1 Installation Windows XP 13 Click the OK button in the Install From Disk dialog 14 Click the Next button in the Add Hardware Wizard 15 Click the Next button once again in the Add Hardware Wizard 16 Click the Continue Anyway button Hardware Installation The software you are installing for this hardware Fujifilm USB Device Managment Driver has not passed Windows Logo testing to verify its compatibility with Windows XP Tell me why this testing is important Continuing your installation of this software may impair or destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing Continue Anyway STOP Installation 17 Click the Finish button to complete the driver instal
20. Safety instructions 2 4 Recycling information 2 4 Recycling information The equipment must be clean from contaminants before decommissioning and all local regulations must be observed with regard to waste disposal Disposal general instructions When taking the ImageQuant LAS 4000 mini out of service the different materials must be separated and recycled according to national and local environmental regulations Recycling of hazardous substances The lamps in the ImageQuant LAS 4000 mini may contain mercury vapour These must be disposed of in manner compliant with local regulations Disposal of electrical components Waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of the equipment 24 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 3 Installation 3 Installation This chapter provides information regarding installation of the ImageQuant LAS 4000 mini 3 1 Site requirements The following table gives a summary of power supply and environmental requirements For detailed information refer to Section 7 1 Main specifications on page 70 Parameter Requirement Power supply 13 A 100 120 V or 10 A 200 240 V Line frequency 50 to 60 Hz Ambient temperature 15 C to 28 C fluctuation 10 C h Storage 25 C
21. Turn on the ImageQuant LAS 4000 mini and computer and any auxiliary equipment Note The ImageQuant LAS 4000 mini and computer may be switched on in any order 2 Start the ImageQuant LAS 4000 mini Control Software Result A status message is displayed while the ImageQuant LAS 4000 mini Control Software is starting 3 Once the software has started wait for the CCD camera to cool The status of the CCD is shown in the main ImageQuant LAS 4000 mini Control Software window When ready the status will change from NOT READY to READY NOT READY F 200 C Chemiluminescence Note When the CCD camera is ready for use the Power LED lamp on the IDX lights blue Note Under normal operation conditions the cooling temperature of the CCD camera is set to 25 C Note The Method Tray position and Focusing may be set even if the CCD is not ready A sample can be placed during the time taken for the CCD to cool 4 2 2 Preparing the ImageQuant LAS 4000 mini for exposure Changing the Epi lights This section describes how to change a set of Epi lights Note The white Epi light is a single light that is installed on the left Remove any Epi light from the right hand side when installing it 1 Ensurethatthe ImageQuant LAS 4000 mini is turned off and open the door If no Epi light is currently in place inside the instrument proceed to step 3 2 Remove screw A slide the Epi light gently outwards and unhook it from the hanger to remove it Epi l
22. a chemiluminescent exposure used for overlaying a marker image 6 Setting state CCD The state of the CCD and exposure type 7 Setting state IDX Current settings of tray position and illumination inside the dark box 8 Method Tray position Set the detection method and tray position 9 Focusing Adjust the focus 10 Start Start the exposure Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB TT 2 Safety instructions 2 Safety instructions This chapter describes safety precautions safety labels and emergency procedures for the ImageQuant LAS 4000 mini in addition to information on the safe disposal of the instrument 2 1 Safety precautions Introduction The ImageQuant LAS 4000 mini is powered by mains voltage and is used to image samples that may be hazardous Before installing operating or maintaining the equipment you must be aware of the hazards described in the user documentation Follow the instructions provided to avoid personal injury or damage to the equipment General precautions WARNING Do not use the equipment if smoke strange noises or strange odors can be perceived or if the equipment becomes unusually hot This may result in fire or electric shock Stop using immediately turn off the power switch and unplug the equipment from the power outlet Contact your local GE Healthcare representative to request repair WARNING Do not allow liquids flammable materials or metallic
23. below and turn it clockwise to attach it to the thread groove on the inside surface of the lens hood attached at the end of the LAS High Sens lens 36 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 3 Installation 3 5 Installation of hardware 3 5 3 Installing the lens and manual filter changer Be sure to insert the end of the manual filter changer in the filter stopper on the back of the instrument If the end of the manual filter changer is not properly inserted in the filter stopper the filter changer may wobble Filter stopper 10 Attach the angle adjustment gauges to the Epi light brackets and turn the screws to fix the brackets in position 3 5 4 Attaching a filter to the manual filter changer This section describes how to attach an optical filter to the filter frame and place it under the lens For instructions on installing the manual filter changer refer to Section 3 5 3 Installing the lens and manual filter changer on page 34 1 Screw the desired filter into the filter frame Filter frame Filter 2 Slide the filter frame into position under the lens in the manual filter changer 3 5 5 Installing Epi light sources This section describes how to install a set of Epi lights Note The white Epi light is a single light that is installed on the left Remove any Epi light from the right hand side when installing it 1 Ensure that the ImageQuant LAS 4000 mini is turned off and open the door
24. countermeasures have been taken fill in the necessary items and the error code in the Service report fax sheet at the end of the User Manual and then contact your GE Healthcare representative 60 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 6 Troubleshooting 6 5 Message ImageQuant LAS 4000 hardware error occurred Content UV exposure error Countermeasure If the problem remains restart the ImageQuant LAS 4000 mini and ImageQuant LAS 4000 mini Control Software SensKey SensCode 8000 2 9100 Temperature adjustment er ror Turn off the ImageQuant LAS 4000 mini power for a while After that restart the ImageQuant LAS 4000 mini and ImageQuant LAS 4000 mini Control Software Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 61 6 Troubleshooting 6 5 Message ImageQuant LAS 4000 hardware error occurred SensKey SensCode Content Countermeasure B004 Focus error during startup Restart the ImageQuant LAS 4000 mini and diagnosis ImageQuant LAS 4000 mini Control Software B008 Iris error during startup diag nosis B010 Epi light source error during startup diagnosis B020 UV transluminator error dur ing startup diagnosis BO A combination of errors B004 3 except described to B020 above are generated above during startup diagnosis B104 Focus error B108 Iris error B110 Transmitted light source error B120 UV tran
25. ction 1 Select the Exposure Type from the drop down list Exposure Type Pl w Precision Exposure mE B Oo Auto Repetition Mar Program Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 47 4 Operation 4 3 Performing an exposure 4 3 4 Set exposure type and exposure time Step Action 2 Select Auto or Manual for Exposure Time Auto Exposure Type Exposure Time Auto O Manual Note Automatic exposure time setting may not be possible depending on the sample type and method e Manual Select an exposure time from the drop down list or enter the exposure time manually The exposure time can be set from 0 01 seconds up to 30 hours Exposure Type Exposure Time O Auto Manual o NUNENEN Sensitivity Add Digitiz ON Flat frame 1j8sec Auto 1 15sec 4 3 5 Set exposure sensitivity The sensitivity can be improved by binning multiple pixels into one larger pixel Smoothing by interpolation then increases the number of pixels for the final image The sensitivity increase in order Standard High Super and Ultra 48 Sensitivity No pixels binned WxH No pixels final image W x H High Resolution 3072 x 2048 3072 x 2048 Standard 1536 x 1024 1536 x 1024 High 768x512 1536 x 1024 Super 384 x 256 1536 x 1024 Ultra 192 x 128 1536 x 1024 High Binning 768 x512 768x512 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 4 Operation
26. d by white light illumination A super CCD camera of 3 2 megapixels is used An effective resolution of 6 3 megapixels can be obtained by the image processing procedure Internal cooling of the CCD contributes to a low thermal noise level resulting in greater sensitivity The F0 85 LAS High Sens lens has remote focus and iris Chemiluminescence is detected at high sensitivity using a four step binning algorithm Fluorescence is detected at high sensitivity using either a UV transilluminator or blue or UV Epi light sources Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 1 Introduction 1 3 The ImageQuant LAS 4000 mini mage capture and analysis is simplified using the ImageQuant LAS 4000 mini Control Software in combination with GE Healthcare s analysis software ImageQuant TL ImageQuant LAS 4000 mini hardware components The following illustrations and table show the main hardware components of the ImageQuant LAS 4000 mini Intelligent dark box IDX IDX interior Part Name Description 1 Camera head CCD cooling and image data output 2 Intelligent dark box IDX Dark box 3 Lens F0 85 43 mm LAS High Sens lens 4 Epi light source Blue Epi light 460 nm UV Epi light 365 nm White Epi light 5 Trans light source 312 nm UV transilluminator optional 6 Filter Y515 605DF40 EtBr filter for detecting EtBr 510DF10 filter for detecting GFP
27. eQuant LAS 4 i m Name Size Type Date modif Location Files Currently on the Disc 2 Recent Places rj USB Control i File Folder USB Function File Folder Lu Computer File name int Files of type Setup Information inf 30 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 3 Installation 3 4 Installation of the software 3 4 2 Installation Windows Vista 10 Select the file DevMng and click the Open button Locate File Lookin J USB Control oid Name Date modif Type Size I 5 GEL Recent Places Desktop Computer A Network File name DevMng Em Filles of type ae 11 Click the OK button in the Install From Disk dialog 12 Click the Next button in the Add Hardware dialog 13 Click the Next button once more in the Add Hardware dialog 14 Click Install this driver software anyway Wi Windows Security xl i2 Windows can t verify the publisher of this driver software gt Don t install this driver software You should check your manufacturer s website for updated driver software for your device 1 gt Install this driver software anyway i Only install driver software obtained from your manufacturer s website or disc Unsigned software from other sources may harm your computer or steal i information v See details 15 Click the Finish button 3 4 2 2 Installation of the USB Function dr
28. eaning Countermeasure The wait time or exposure time of The wait time or exposure Change the wait and exposure image xx is out of range time is not appropriate for times to appropriate values Please change the specified image Method Tray Position button Error message Meaning Countermeasure Cannot identlify the light source The light source could not Ensure that the light source is Please check that the light unit isin Pe identified installed correctly stalled correctly Cannot find the appropriate filter for The appropriate filter is not Make sure the correct filter for the method Please install an appro in place the method is in place and priate filter registered in the software Option menu FlatFrame Calibration Error message Meaning Countermeasure The door was opened during expo The door was opened dur Close the door again and per sure Flat frame calibration failed ing flat frame calibration form the flat frame calibration again An error occurred during flat frame Flat Frame calibration im Check that the correct sample calibration for Cy3 tray position 4 age exposure failed tray and calibration plate are Please check that the correct tray in place and re calibrate and calibration plate were used Cannot start flat frame calibration The CCD has not been cali Performa flat frame calibration because the CCD calibration for High brated Resolution mode does not
29. ed to corrosive gas Do not install the equipment in a dusty environment Dn Ui f N Do not install the equipment in a place constantly or excessively exposed to vibration or impacts 7 Donotinstall the equipment in a place exposed to direct sunlight Operation site Indoors Maximum operating altitude 2000m Overvoltage category category ll Transient overvoltage Rated pollution applied Pollution Degree 2 Analysis PC interface USB 2 0 Do not connect the ImageQuant LAS 4000 mini USB connector to a computer not certified with UL60950 1 UL listed and IEC60950 1 Other Noise 70 dB A or lower Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 73 7 Reference information 7 2 Minimum computer requirements 7 2 Minimum computer requirements Operating system Windows XP SP3 or Windows Vista Business SP1 32 bit Memory More than 1 GB Processor Intel Core 2 Duo processor HD More than 80 GB USB port version USB 2 0 Optical drives DVD ROM Monitor resolution More than 1280 x 1024 pixels 7 5 Literature For further information on the ImageQuant LAS 4000 mini and the ImageQuant LAS 4000 mini Control Software refer to the ImageQuant LAS 4000 mini User Manual 7 4 Ordering information For ordering information visit www gelifesciences com quantitative_imaging 74 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB For local
30. era head Use a soft dry cloth to clean them 5 4 UV transilluminator Use an air blower to remove dust Greasy residues can be cleaned with a soft cloth dampened with mild detergent then wipe with a soft dry cloth WARNING Wear gloves while cleaning There may be residues of hazardous chemicals 5 5 Regular inspections Regularly inspect the ImageQuant LAS 4000 mini to ensure the following The power supply plug is firmly secured in the power outlet The power cable and supply plug do not become overheated The power cable is not damaged in any way The ventilation holes in the equipment are free from dust and dirt Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 6 Troubleshooting 6 Troubleshooting This chapter describes various problems that can foreseeably occur with the ImageQuant LAS 4000 mini and ImageQuant LAS 4000 mini Control Software Suggestions of possible countermeasures are given Take the following procedures if an error occurs 1 Take note of the error code and error message on the monitor 2 Turn off the power to ImageQuant LAS 4000 mini and the analysis PC then turn them on again after about ten seconds 3 If the error persists fill in the necessary items and the error code in the Service report fax sheet at the end of the ImageQuant LAS 4000 mini User Manual and then contact your GE Healthcare representative Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 55
31. esdnE Ru Ue tM RU nanan a NR ARR 32 med Attaching the camera egeo RC DE MEME EE ENEM EM RENE 33 Jor Connecting the camera head to the COMputer cescncenmavetaatevbangrier etti el ttes cate gas 34 3 5 3 Installing the lens and manual filter changer sss msertetii SOR ttd hn iS tant rt tein 34 3 5 4 Attaching a filter to the manual filter Changer user trac in tradu rare ala qua e ted innate 37 355 Installing Epi light SOUTCES siniiiniioriirerieriiiori teinien AEAEE eS 37 G IODSFOLIOH ene A ATO M 39 au SCIELO ICM Vile W seseina n R ANE EM Neb MM MM RE M dE 39 42 PEPA IIO e e EE E S E E EDU Ha dE 39 4 2 1 Starting the ImageQuant LAS 4000 mini and Control Software s 39 422 Preparing the ImageQuant LAS 4000 mini for exposure soit stirbt 40 423 Placing the SAMP MERO RC 42 A deformi dy SXDOBU B NEE 44 LC WEN oio cC X 45 432 Choose method REO QU DO SON S oett od vada dante tan e d ap Oda did a raa V 45 43 3 F c sadj stMent ERROR RO K t 47 4 3 4 Set exposure type and exposure time eese ipeo aetas bmi edet cov n tdeo pe Su dtd 47 4 3 5 SELONDOSUPE SODSIUUIEM assdesua SURDAUEUERURDERAURU MUI REUNRRAR WEEK RU TURN REMERON ER REINO 48 4 3 6 eri Rc 49 427 SWE 2a ccsisp MEUM MEE EM HR an M UO SEO Te En 50 44 After oci c 51 0 MOMPCNONCS suiincunumnncnnninnncnmnkiiinnmian ee nies 53 5 1 ImageQuant LAS 4000 mini ssssssseccecssssesccessons
32. exist Please run CCD calibration first Option menu CCD Calibration Error message Meaning Countermeasure The door was opened during expo The door was opened dur Perform the CCD calibration sure This image may be inaccurate ing exposure sotheimage again may not have been ex posed correctly 68 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 6 Troubleshooting 6 6 Error messages and countermeasures Option menu CCD Calibration Pro Error message Meaning Countermeasure Cannot use the flat frame calibration The FlatFrame Calibration Use the CCD Calibration option for the following settings Light none Pro function cannot be Filter No1 FUJINON VRF43LMD lens used in chemilumescent mode Cannot continue with the automatic The automatic exposure Change the settings appropri exposure function The sample may functionisunabletocalcu ately or perform an exposure be out of the photometric range or late a correct exposure in Manual mode a setting may need to be adjusted time Help menu Error message Meaning Countermeasure Failed to open the User Manual The PDF documents can Ensure that the documentation Please note that a PDF reader e g not be opened and PDF reader software is in Adobe Reader is needed stalled Failed to open End User Licence Agreement Please note that a PDF reader e g Adobe Reader is needed Failed to ope
33. f exposure to be performed Detection Sample type Tray Chemiluminescence Membrane Epi tray Bioluminescence Titer plate NP tray Fluorescence Gel UV Trans illumination UV trans tray Gel Epi illumination Epi tray Membrane Epi tray Digitization Membrane Epi tray Gel Coomassie silver stain White trans tray 42 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 4 Operation 4 2 Preparation 4 2 3 Placing the sample 2 Place a sample on the sample tray For Epi tray a Place the sample directly on the tray For UV or White Trans trays a Cut out a gel sheet slightly larger than the sample size b Place the gel sheet on the Trans tray Gel sheet c Place the sample on the gel sheet Sample Gel sheet Note The gel sheet can be used repeatedly After use wash it with mild detergent rinse with water then dry well A gel sheet can be reused about 20 times Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 43 4 Operation 4 2 Preparation 4 2 3 Placing the sample 3 Choose the exposure size and tray position For Epi and Trans trays Thereadable area varies depending on the tray position Place the sample in position according to its size 144mm x 96mm 105mm x 70mm a 90mm x 60mm Position 0 Position 1 TIP On the Epi tray there are round dents marked on the tray for positioning the sample Line up the sample using the appropriate dents
34. fter quit 2 OK Cancel TIP If Keep the CCD cooling after quit is selected the cooling temperature of the CCD will be maintained and you can immediately start using it upon simply restarting Image Quant LAS 4000 mini Control Software This is useful if more samples will be exposed later in the day 3 Switch off the computer 4 Turn off the ImageQuant LAS 4000 mini power switch 52 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 5 Maintenance 5 Maintenance To ensure optimal performance the ImageQuant LAS 4000 mini and accessories should be maintained regularly This chapter describes the maintenance that should be performed on a regular basis NOTICE eo Before performing any maintenance on or inside the ImageQuant LAS 4000 mini be sure to turn off the power switch and remove the power plug from the electrical outlet 5 1 ImageQuant LAS 4000 mini Use a soft dry cloth to clean the exterior of the ImageQuant LAS 4000 mini For marks that are hard to remove wipe with a soft cloth slightly dampened with a neutral detergent then wipe with a new clean dry cloth To clean the interior of the instrument use a non fluorescent cleanser for biochemical use If using a neutral domestic detergent the detergent residue may fluoresce when exposing images NOTICE eo Do not use organic solvents These may damage the surface 5 2 Sample trays After using a sample tray wash
35. g countermeasures have been taken fill in the necessary items and the error code in the Service report fax sheet at the end of the User Manual Phenomenon ImageQuant LAS 4000 mini Control Software cannot recognize Image Quant LAS 4000 mini Cause USB cable connection failure Countermeasure Connect the USB cable correctly to the camera head and PC Power cable or camera cable connection failure Connect the power cable and camera ca ble correctly The CCD temperature indication does not change to READY or its value does not approach the set temperature af ter a sufficient time about 15 minutes has elapsed since the start of cooling The ambient temperature is too high Set the ambient temperature to 28 C or less The CCD temperature is set too low Set the cooling temperature of the CCD properly USB cable connection failure Connect the USB cable correctly The camera head ventilation holes are blocked Confirm that nothing is blocking the venti lation holes Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 59 6 Troubleshooting 6 4 Problems with ImageQuant LAS 4000 mini Control Software Phenomenon No focus image is displayed in the Fo cusing mode Cause USB cable connection failure Countermeasure Connect the USB cable correctly Brightness of reading soft ware is too high or low Adjust the Brightness in the reading soft ware
36. he door was opened The door was opened dur The image may be incorrectly during exposure ing exposure exposed Perform the exposure again Start button after exposure Error message Meaning Countermeasure No image file was saved Do you No images were saved Press OK to discard the want to continue recorded imagels or Cancel to go back and save the images The save and launch function failed Failed to launch the exter Choose an application to nal application The desig launch in Launch Application nated filecannotbefound in Preference in the Edit menu Program settings button Error message Meaning Countermeasure Cannot save There are too many exist Remove some program tem The maximum number of registered Ih program template files plate files that are no longer program templates has been required reached A program template with the same A file with the same name Change the filename or choose name already exists Do you wantto already exists to overwrite the older file overwrite it You cannot specify the first character Filename starts with anin Supply a valid file name of template name as or Please valid character start with another character Please input a filename The filename is blank Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 67 6 Troubleshooting 6 6 Error messages and countermeasures Error message M
37. hing and eyewear UV radiation can cause severe burns and long term injury to the skin and eyes gt Excessive exposure of the skin can cause premature aging allergic reactions and cancer Medication or cosmetics may increase sensitivity to UV radiation Consult a physician before using this product if using medication if you have known skin disorders or if you are especially sensitive to sunlight Excessive exposure of the eyes can result in permanent damage to your vision CAUTION Do not stare at the light emitted from the LED lights sources This may impair vision Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 13 2 Safety instructions 2 1 Safety precautions Operation WARNING Do not use the equipment with a power supply other than that recommended Fire and electric shock could result WARNING Do not use the equipment within or near a sink or in humid or dusty environments Fire and electric shock could result WARNING If thunder can be heard do not touch the power supply plug or electric shock could result WARNING Connect the power supply directly to a grounded wall power outlet The use of extension cords or multiple loads on one electrical outlet could result in fire and electric shock CAUTION Do not use the same power supply as that of large equipment such as an air conditioner or centrifuge Malfunction could result CAUTION D
38. iew in the upper left corner 2 Open the Add Hardware tool 3 In the Add Hardware dialog click the Next button 4 Select Install the hardware that manually select from a list Advanced and clickthe Next button Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 29 3 Installation 3 4 Installation of the software 3 4 2 Installation Windows Vista 5 Select Show All Devices and click the Next button From the list below select the type of hardware you are installing If you do not see the hardware category you want click Show All Devices Common hardware types L3 Display adapters Cj IDE ATA ATAPI controllers Ge IEEE 1284 4 compatible printer GER IEEE 1284 4 devices IEEE 1394 Bus host controllers E Imaging devices IF Infrared devices Wl adis Cantar Eutender 6 Click the Have Disk button 7 Insert the ImageQuant LAS 4000 mini Control Software CD and click the Browse button 8 Select to install the driver from the ImageQuant LAS 4000 mini Control Software CD Lookin 9 DVD CD RW Drive D ImageQuant LAS 40e F c Gly ERAI a ae te modif Location zal D Network Recent Places airin d Public 7 Computer Floppy Disk Drive A Local Disk C Jo Windows System32 we STORE N GO E iM Computer Network File name inf Files of type Setup Information inf 9 Open the USB Control folder DVD CD RW Drive D Imag
39. ight source Screw A 40 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 4 Operation 4 2 Preparation 4 2 2 Preparing the ImageQuant LAS 4000 mini for exposure 3 Putthe new Epi light source on the guide Then slide the light source inwards to attach it This will connect the light to the power connector 4 Fix the Epi light source into position using screw A Note The Epi lights are clearly labelled L for the left and R for the right sides e install the Epi lights in matching pairs only If Epi lights with different wavelengths are used the intended image will not be obtained Changing or installing a filter An optical filter can be inserted under the lens in the manual filter changer as follows 1 Remove the filter frame from the manual filter changer 2 Ifa filter is already in place unscrew it from the filter frame and place it back in its cover 3 If anew filter is desired for the exposure screw it in place in the filter frame Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 41 4 Operation 4 2 Preparation 4 2 2 Preparing the ImageQuant LAS 4000 mini for exposure Filter frame 4 Slide the filter frame into position under the lens in the manual filter changer 4 2 3 Placing the sample This section describes how to choose an appropriate sample tray and how to place the sample for exposure 1 Select a sample tray suitable for the type o
40. ing Started with ImageQuant LAS 4000 mini 28 9607 61 AB 57 6 Troubleshooting 6 2 Strange sounds or odors are perceived Phenomenon ImageQuant LAS 4000 mini beeps on and off four times at intervals of three minutes and the Busy LED blinks Cause The door is open Countermeasure Close the door The subsequent processing should start ImageQuant LAS 4000 mini beeps on and off three times and the ERROR LED lights A hardware error oc curred See the countermeasures for the SensKey and SensCode numbers displayed on screen of the analysis PC in Section 6 5 Message ImageQuant LAS 4000 hardware error occurred on page 60 ImageQuant LAS 4000 mini generates ImageQuant LAS 4000 Turn off the ImageQuant LAS 4000 mini power a strange odor mini failure switch immediately and contact your GE Healthcare representative ImageQuant LAS 4000 mini emits ImageQuant LAS 4000 Turn off the ImageQuant LAS 4000 mini power smoke mini failure switch immediately and contact your GE Healthcare representative 6 3 Problems with ImageQuant LAS 4000 mini If the error persists even after the following countermeasures have been taken fill in the necessary items and the error code in the Service report fax sheet at the end of the User Manual Phenomenon startup diagnosis LED lights on ImageQuant LAS 4000 mini do not light during Cause failure Power cable installation Countermeasure Connect the p
41. ironment meets the required specifi cations Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB Phenomenon age 6 Troubleshooting 6 1 Problems with the exposed image Cause Countermeasure Light source uneven Check that both left and right Epi light ness sources are correctly in place and that they are of the same type Unevenness is seen on the im Alampinthe UVtran Turn the power off and contact your GE silluminator is broken Healthcare representative The sample trayisnot Place the tray correctly at the appropriate correctly positioned tray position at the appropriate tray position The Flat Frame is not Expose the Flat Frame again correctly exposed posed using UV trans tray There is noise on an image ex Degradation of UV Purchase a new UV trans tray trans tray 6 2 Strange sounds or odors are perceived Phenomenon Cause Countermeasure The camera head generates a strange Camera head failure Turn off the ImageQuant LAS 4000 mini power noise switch immediately and contact your GE Healthcare representative The camera head generates a strange Camera head failure Turn off the ImageQuant LAS 4000 mini power odor switch immediately and contact your GE Healthcare representative The camera head emits smoke Camera head failure Turn off the ImageQuant LAS 4000 mini power switch immediately and contact your GE Healthcare representative Gett
42. it in water using a soft sponge dipped in a neutral detergent After cleaning leave the tray to air dry WARNING Wear gloves while cleaning There may be residues of hazardous chemicals NOTICE Do not use organic solvents These may damage the surface Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 53 5 Maintenance 5 2 Sample trays NOTICE o Do not use abrasive cleaning materials such as a scouring pad for cleaning This may scratch the surface 5 5 Lens filters NP tray and Epi light sources Use a soft dry cloth only to clean these components To maintain image quality when cleaning optical components the following points should be observed Never touch the optics lens camera head window filter surfaces or Epi light windows with bare hands Use an air blower to remove dirt or dust If the optics have been touched accidentally wipe the soiled surface using lens cleaning paper dipped in lens cleaner solution Any grease allowed to remain on the surface may become difficult to remove cause degeneration and possibly molding Do not adjust fixing screws or tie rings on the components unnecessarily This may cause the performance to deteriorate Use the protective caps on the camera head and lens whenever they are not installed in the ImageQuant LAS 4000 mini Store these protective caps carefully Do not allow dirt or dust to accumulate on the inlet and outlet vents of the cam
43. iver Windows VISTA Note During software installations you may be asked to confirm your actions in a dialog with thetext Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation 1 Connectthe computer and the ImageQuant LAS 4000 mini with a USB cable and turn ON the power switch of ImageQuant LAS 4000 mini Result The ImageQuant LAS 4000 mini will automatically be detected by the computer and the Plug and Play function in Windows Vista starts In the Found New Hardware dialog select Locate and install driver software recommended Insert the ImageQuant LAS 4000 mini Control Software CD and click the Next button in the Found New Hardware dialog Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 31 3 Installation 3 4 Installation of the software 3 4 2 Installation Windows Vista 4 Select Install this driver software anyway 5 I Windows Secunty Windows can t verify the publisher of this driver software Don t install this driver software You should check your manufacturer s website for updated driver software for your device gt Install this driver software anyway Only install driver software obtained from your manufacturer s website or disc Unsigned software from other sources may harm your computer or steal information v See details A successful installation message appears Click the Close bu
44. justment screws and remove the Epi light source brackets both on the right and left from the angle adjustment gauge then pivot the Epi light source brackets down Angle adjustment gauges Epi light source angle adjustment screws 4 Remove the end cap 5 Insert the lens according to the lens guide with the red points in front of you 34 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 3 Installation 3 5 Installation of hardware 3 5 3 Installing the lens and manual filter changer Re po d E N 7 Attach the lens cable Turn the knurling ring until it is securely fixed Connector of the lens cable Note Be sure to connect the lens cable otherwise the High Sens lens may not be recognized and may cause failure of the equipment Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 35 3 Installation 3 5 Installation of hardware 3 5 3 Installing the lens and manual filter changer 8 Remove the lens cap a Lens cap Lek adi de C 9 Mount the manual filter changer on the end of the lens paying attention to the following e Check the top and bottom sides of the manual filter changer The manual filter changer will be attached to the thread groove on the inside surface of the lens hood attached at the end of high sensitivity lens Top LAS High Sens lens side Bottom Look up the fringe from
45. lation 3 4 1 2 Installation of the USB Function driver Windows XP 1 Connect the computer and the ImageQuant LAS 4000 mini with a USB cable and turn ON the power switch of ImageQuant LAS 4000 mini Result The ImageQuant LAS 4000 mini will automatically be detected by the computer 2 Inthe Found New Hardware Wizard dialog choose No not this time and click the Next button Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on your computer on the hardware installation CD or on the Windows Update Web site with your permission Read our privacy policy Can Windows connect to Windows Update to search for software Q Yes this time only O Yes now and every time connect a device Click Next to continue 3 Insert the ImageQuant LAS 4000 CD then select Install the software automatically Recommended and click the Next button 4 Click the Finish button to complete the installation 3 4 1 3 Installation of ImageQuant LAS 4000 mini Control Software Windows XP 1 Insert the ImageQuant LAS 4000 mini Control Software CD 2 Locate and double click the file ImageQuant LAS 4000 msi 3 In the ImageQuant LAS 4000 InstallShield Wizard click the Next button 4 Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative see the back cover of this manual for contact information Select I accept
46. light source is installed Countermeasure Check that the intended light source s are correctly in place and that correct method is chosen Cannot start the exposure Flat frame calibration has not been performed Please run flat frame calibration for the selected settings Cannot start the exposure Flat frame calibration has not been performed for digitization epi illumination Please run flat frame calibration for digitization epi illumination Flat frame calibration data was not found There is no Flat Frame file that satisfies the exposure conditions Create a correction file that satisfies exposure conditions using the Flat Frame Calibra tion function in the Option menu Cannot start the exposure CCD cali bration has not been performed Please run CCD calibration for the selected exposure time Calibrate now and continue CCD calibration is not ready for exposure Click the OK button to start ex posure CCD calibration for the exposure conditions is initiated Cannot start the exposure CCD cali bration has not been performed Please run CCD calibration for the selected exposure time The CCD is not calibrated Create a correction file that satisfies exposure conditions using the CCD Calibration function in the Option menu Cannot start the exposure Automatic exposure requires CCD calibration Please run CCD calibration for the exposure time range from 1 100 sec to 2 hours
47. me is given in Program Settings Input a valid filename in the Program Settings dialog Please input a valid exposure time The exposure time is not appropriate Choose a suitable exposure time Cannot start the exposure Automatic exposure is only available when using standard sensivity in the digitization method Automatic exposure cannot be used at the sensitivity or resolution In the Digitize Method the Auto function cannot be used when the sensitivity is High Super or Ultra Change the sensitivity setting or expose an image in Manual mode Please input a valid repeat limit count The number of exposures is not approriate in Incre ment or Repetition mode Change the number of expo sures or repeat limits to a suit able quantity CCD Cooling is not stable Dark frame correction will not be ac curate Do you want to continue CCD calibration will not be accurate since the temper ature is not stable when exposing an EtBr sample before the CCD has reached its target cooling temperature Click the OK button if you wish to take an exposure without correction Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 65 6 Troubleshooting 6 6 Error messages and countermeasures Error message Can not start the exposure because the light source does not match the method Please exchange the light source or select another method Meaning The incorrect
48. mini 28 9607 61 AB 5 1 Introduction 1 1 Important user information Intended use The ImageQuant LAS 4000 mini is a camera system that produces digital images of chemiluminescent dyed or fluorescent gels and membranes The ImageQuant LAS 4000 mini is intended for research use only and shall not be used in any clinical procedures or for diagnostic purposes Safety notices This user documentation contains WARNINGS CAUTIONS and NOTICES concerning the safe use of the product See definitions below Warnings WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood Cautions CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to proceed until all stated conditions are met and clearly understood Notices NOTICE o NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Notes and tips Note A Note is used to indicate information that is important for trouble free and optimal use of the product TIP A tip contains useful information that can improve or optimize your procedures Typographical conventions Software items are identified in the text by bold italic text colon separates menu levels thus File Open refer
49. n the Getting Started guide Please note that a PDF reader e g Adobe reader is needed Edit menu preference Print Adjust Error message Meaning Countermeasure The range of value is 90 110 The supplied value isnot Enter a suitable value Please set a suitable value within the appropriate interval Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 69 7 Reference information 7 Reference information 7 1 Main specifications CCD 3 200 000 pixels Fujifilm super CCD Cooling temperature Number of gradations 25 C Recorded image 16 bit Focusing 8 bit Exposure time 1 100 seconds to 2 hours Images can be continuously exposed for up to 30 hours using ImageQuant LAS 4000 mini Control Software Lens High sensitivity lens F0 85 43 mm Wide view lens optional F1 8 24 mm Shading correction Software system Maximum sample size 180 X 120 mm Dynamic range 4 orders of magnitude Maximum image size 12 6MB Light source LED incident Epi illumination class 1 light source Blue Epi light 460 nm UV Epi light 365 nm White Epi light 470 740nm UV transilluminator 312nm Size 322mm X 313mm X 87mm UV transmitted filter size 200mm X 200mm External dimensions Camera head 224 W x 161 H x 252 D mm IDX 440 W x 510 H x 374 D mm 70 Getting Started with ImageQuant LAS 4000 mi
50. ni 28 9607 61 AB 7 Reference information 7 1 Main specifications Weight Camera head 3 4 kg ImageQuant LAS 4000 mini 23 kg excluding the light source filter changer lens and tray LAS High Sens lens 4 5 kg Power requirements Input voltage 100 240 V Voltage variation 10 Phase Single phase Power frequency 50 60 Hz Rated input current 3 0 1 5 A AC power cable Use the cable supplied with the instrument Specifications of cables required for the use of ImageQuant LAS 4000 mini Voltage 100 120 V Plug connector Cable 125 VAC 13 A SJT3 x LEAWG 60 C Power supply cord length Maximum 3 m Voltage 200 240V Plug connector Cable 250 VAC 10A CENELEC OC 3 x 1 0 mm 70 C Power supply cord length Maximum 3 m Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 71 7 ra Reference information 1 Main specifications 72 Installation conditions Placement conditions Free space required around ImageQuant LAS 4000 mini Secure space for maintenance work as follows Operation panel side Right Left Side opposite to operation panel Top Table floor strength 500 mm 500 mm 500 mm 200 mm 1000 mm The allowable load must be 981 N m 100 kg m or higher Other conditions 1 Decideonaninstallation layout taking into consideration the work flow and ancil lary facilities to be used 2 Required con
51. o not block the vents and ensure that the vents are kept free of dust and dirt Blockage of the vents can result in overheating of the equipment and malfunction Place the instrument at least 20 cm away from walls or other equipment to ensure adequate cooling gt l P gt i P gt CAUTION Do not open the door of the instrument too wide and do not apply excessive force to it Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 2 Safety instructions 2 1 Safety precautions CAUTION When opening or closing the door use the opening lever Take care not to catch objects or fingers in the door when closing CAUTION Do not place heavy objects on the equipment These may fall and cause injury CAUTION Only one ImageQuant LAS 4000 mini should be connected to a particular PC Connecting more than one may result in malfunction CAUTION Turn the power switch off before connecting or disconnecting cables otherwise the equipment may malfunction CAUTION Do not connect any lens or light source other than those recommended by GE Healthcare CAUTION Turn the power switch off before attaching or detaching a lens otherwise the equipment may malfunction CAUTION Do not leave the lens hanging from the lens guide It may fall and damage the equipment CAUTION Do not touch the shutter on the camera head Damage to the shutter will impede correct operation
52. od Tray position Method Light Filter Z Iris Chemiluminescence None Through F0 85 Fluorescence O Digitization Epi illumination White Epi illumination Through F2 8 Trans illumination White Trans illumination Through F2 8 Detection MGI eTeTe Chemiluminescence Chemiluminescence Bioluminescence Fluorescence using UV transilluminator 312 nm Fluorescence EtBr Fluorescence using Blue Epi light 460 nm Fluorescence Cy2 GFP Sample digitization using White Epi light Digitization Epi illumination 3 Select Tray position according to the sample size or type 90 x 60 mm Go Le O1 2 E Os NP 4 Click the OK button 46 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 4 Operation 4 3 Performing an exposure 4 3 3 Focus adjustment 4 3 3 Focus adjustment Step Action 1 Click the Focusing button Result The focusing controls appear Ls Function 1 Decrease image brightness 2 Increase image brightness Coarse focus adjustment 4 Fine focus adjustment 5 Return to the main display 6 Click on the image to zoom Note Adjusting the brightness does not influence the actual exposure 2 Check that the sample is correctly positioned and focus the image 3 Click the Return button 4 3 4 Set exposure type and exposure time For a detailed description of the different exposure types refer to the ImageQuant LAS 4000 mini User Manual Step A
53. ower cable correctly locked The ImageQuant LAS 4000 mini door cannot be opened and closed The door cannot be door sensor Foreign objectis present in the locking section or Remove the foreign object damaged The locking section is Contact your GE Healthcare represen tative on The initial diagnosis does not start even if the power is turned The camera coble is not connected correctly Turn the power off and check the camera cable connection Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB Phenomenon A focused image is not displayed Cause failure 6 Troubleshooting 6 3 Problems with ImageQuant LAS 4000 mini Countermeasure Power cable and cam Connect the AC power cable and era cable connection camera cable correctly Connector connection Connect the trans light source by failure Light failure pushing it all the way until it hits the other end Lock the Epi lights in place in the door sensor Foreign objectis present Remove the foreign object Focusing mode Software is not in the Click the Focusing button in the soft ware door is opened The light source is lit even when Interlock failure the ImageQuant LAS 4000 mini Turn off the power immediately and contact your GE Healthcare represen tative 6 4 Problems with ImageQuant LAS 4000 mini Control Software If the error persists even after the followin
54. rror messages Error message The hard disk is full Cannot save Disk capacity is insuffi cient Exposure failed Disk working space is insufficient Meaning Disk space is insufficient Countermeasure Free some disk space Cannot save File I O Error An error occurred during writing the file Check the file name and folder are correct Check that the PC is operating correctly Cannot save The folder is write pro tected The folder is write protected Please change the folder The folder to which the file is to be written is write protected Save to a different folder or cancel the write protection Cannot save A file with the same name is write protected A file with the same name is write protected Cancel the write protection of the file or save to a file with a different name Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 63 6 Troubleshooting 6 6 Error messages and countermeasures Error message Meaning Countermeasure File open error An error occurred while Check the correct file is chosen opening the file Check the PC is operating cor rectly In the worst case the file may have become corrupted Please close the door The IDX door is open Close the door Can not use the characters below for The filename contains in Change the filename excluding the filename valid characters the invalid characters eed Sta
55. rsion curve can be toggled between Linear or Sigmoid 3 The gradation can be altered by dragging with the mouse 50 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 4 Operation 4 5 Performing an exposure 4 3 7 Save the image Step Action 2 Click the Save button or select Save in the File menu to save the image Result The Save dialog opens LAS4000lmage sz e aam My Recent Documents Desktop My Documents My Computer My Network File name Places Save as type Gel Fie Foma vl 2 Choose a folder file name and file format then click the Save button Note gel format A file format developed by GE Healthcare Exposure details are maintained tiff format A 16 bit TIFF format suitable for analysis in external software Exposure details are not maintained 3 Click the Complete button Result The current display returns to the initial screen 4 4 After exposure If the ImageQuant LAS 4000 mini will not be used for some time it should be switched off as follows Step Operation Select Quit from the File menu I ImageQuant LAS 4000 EM Edit View Option Page Setup nt Quit e T FTELISIUIT Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 51 4 Operation 4 4 After exposure Step Operation Select Stop the CCD cooling now Click the OK button Stop the CCD cooling now Keep the CCD cooling a
56. rt up Error message Meaning Countermeasure The environmental temperature is The ambient temperature Turn off the the ImageQuant too high to cool the CCD is too high for the CCD to LAS 4000 mini reduce the air cool temperature around the instru ment and then restart Image Quant LAS 4000 mini and Im ageQuant LAS 4000 mini Con trol Software Components in ImageQuant LAS The default settings have Changethe method and or the 4000 have changed been changed tray position in the Please change the exposure method Method Tray Position dialog Please change settings in Method Tray Position window 64 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB Start button starting exposure Error message The interval time must be greater that 10 seconds when using the In crement Repetition mode The maximum exposure time when using the EtBr method is 10 minutes The maximum exposure time when using the UV light is 10 minutes The maximum interval time is 2 hours The maximum exposure time is 30 hours The maximum interval time when using the EtBr method is 60 seconds The maximum interval time when using the UV light is 60 seconds Meaning The time value is inappro priate 6 Troubleshooting 6 6 Error messages and countermeasures Countermeasure Enter an appropriate time value Please input the file name using the program settings window No file na
57. ry use EN 61326 1 EMC emissions and immunity require Harmonized with VCCI Class A ments for electrical equipment for mea 2004 108 EC FCC Part 15 B Class A surement control and laboratory use ICES 003 Class A EN ISO 12100 1 12100 2 Safety of machinery Basic concepts Harmonized with general principles for design 2006 42 EC Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB N 1 Introduction 1 2 Regulatory information Standard Description EN ISO 14121 1 14121 2 Safety of machinery Principles of risk Harmonized with assessment 2006 42 EC CE Marking The CE marking and the corresponding Declaration of Conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in the user documentation and used in the same state as it was delivered from GE Healthcare except for alterations described in the user documentation Regulatory compliance of connected equipment A 6 C ny equipment connected to the ProductNameHW should meet the safety requirements of EN 1010 1 IEC 61010 1 or relevant harmonized standards Within EU connected equipment must be E marked 1 3 Thel mageQuant LAS 4000 mini Feature overview The following features are offered by the ImageQuant LAS 4000 mini Digitized images of stained gels membranes or films can be obtaine
58. s to the Open command in the File menu Hardware items are identified in the text by bold text e g Power switch 6 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 1 Introduction 1 2 Regulatory information 1 2 Regulatory information This section describes the directives and standards that are fulfilled by the ImageQuant LAS 4000 mini Manufacturing information The Declaration of Conformity includes the following information Requirement Content Name and address of manufacturer GE Healthcare Bio Sciences AB Bjorkgatan 30 SE 751 84 Uppsala Sweden Name and ID of notified body INTERTEK SEMKO AB NB 0413 Place and date of declaration Uppsala Sweden May 2009 Identity of person authorized to sign Declaration See EC Declaration of Conformity of Conformity CE Conformity This product complies with the European directives listed in the table by fulfilling the corresponding harmonized standards A copy of the Declaration of Conformity is available on request 2006 42 EC Machinery Directive MD 2006 95 EC Low Voltage Directive LVD 2004 108 EC ElectroMagnetic Compatibility EMC Directive International standards This product fulfills the requirements of the following standards Standard Description Notes EN 61010 1 IEC 61010 1 UL Safety requirements for electrical equip 61010 1 IEC 61010 2 81 ment for measurement control and lab CAN CSA C22 2 No 61010 1 orato
59. sescresonssccersssusussceesonssessesssnssssscessnusssecegsanusesececensssesecesenseseseceesaseceseeeee 53 52 Samplet IVS oai EGER HE GO Eno un UE GRE M RM OH UEM d Hp DERE IOS 53 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 3 Table of Contents 6 5 3 Lens filters NP tray and Epi light sources sssssssssssssssssssesececesscesnsstseseeecesesssssseseceeesessunmsesteseceesenssnsnnseseseeeees 54 Sa Ei agarre dee 54 55 Ie gio ilo Rn 54 MANUS pered i IAY RC T PAR IR UE RNC 55 6 1 Problems with the exposed iMage sce aote qa tetti dE REED EC MB ETUPA Opmn aTNE 56 6 2 Strange sounds or odors are perceived ea namnpteiu mp vt viaa advent dep dert ron dac uso iis id 57 amp 3 Problems with ImageQuant LAS 4000 Mini Gies beet tette RI THEE E HISEH Hi HIE m netten 58 6 4 Problems with ImageQuant LAS 4000 mini Control Software etn 59 6 5 Message ImageQuant LAS 4000 hardware error occurred teens 60 66 Error MESSAGES arid couritermiBDSf BS sisisi ctn cette tite otl atteid 63 Reference IXTGETTI EDO Does Ga nd ranae Mr rtm DM MIU GP SM M ELM EPUM 70 gb MaM SDERCITICEIU DIS cac BUT UNUNRU MEAE UAS EY BON HERHEEHS MEA UIEENEH UIDIMOUEMUTEU UNEEEL Ho FEREHEEU ESHAER UEBER CHEIESIEAKEUR 70 7 2 MINIMUM computer requirements scsccccssrssesescrenneseseersnsusseserenesssssgressssssecsesesussssecusasnsescesssssssssegsanusesescseassoreegeetess 74 re ME usi a 74 D Ord
60. silluminator error Bl Errors B104 to B120 above except described are generated in combina above tion A000 Communication error be Restart the ImageQuant LAS 4000 mini and tween camera head and Im ImageQuant LAS 4000 mini Control Software ageQuant LAS 4000 mini A100 Communication retry error between camera head and ImageQuant LAS 4000 mini A200 Communication parameter error between camera head and ImageQuant LAS 4000 4 mini D000 Memory error during startup diagnosis D100 Image data memory error during startup diagnosis D2 Shutter error during startup diagnosis D300 Time out error of startup diag 62 nosis Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 6 Troubleshooting 6 5 Message ImageQuant LAS 4000 hardware error occurred Countermeasure SensCode Content D600 Interrupt processing error D700 LED connection error D800 Board fan error D900 Camera head fan error DCOO Shutter error during exposure E000 OS system call error 6 6 Error messages and countermeasures The tables below describe error messages that can foreseeably be generated by the software These are categorized according to the operation that can cause the error along with an explanation of the error and an appropriate countermeasure For a description of hardware errors see Section 6 5 Message ImageQuant LAS 4000 hardware error occurred on page 60 General e
61. struction and electricity air conditioning work must be completed in advance 3 Itis not desirable to have a heat source on the IDX right side face where there is an air intake and outlet even if the other environmental requirements have been met 4 Donotinstall the equipment near a window to avoid direct sunlight Also attach a blind to nearby windows 5 Donot place objects near the power outlet so that you can disconnect the power cable from the socket in case of emergency Floor vibration conditions 1 Operating time 2 Non operating time Oscillation Impact Oscillation Impact 0 03G 5 to 60 Hz 16 0 4G 5 to 60 Hz 26 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 7 Reference information 7 1 Main specifications Environmental conditions Operating temperature humidity conditions Temperature 15 C to 28 C with temperature fluctuation below 10 C per hour or lower Humidity 3096 to 70 RH no dew condensation When the above conditions cannot be satisfied take appropriate actions Transportation storage conditions Temperature 25 C to 70 C Humidity 596 to 10096 RH no dew condensation Installation location conditions 1 Donotinstall the equipment where the temperature can vary widely Do not install the equipment near a heat source such as a radiator Do not install the equipment where it may get wet or flooded Do not install the equipment where it may be expos
62. ting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 3 Installation 3 5 Installation of hardware 3 5 1 Attaching the camera head 3 5 1 Attaching the camera head The camera head is attached to the IDX as follows 1 Install the camera head while pushing the lens release button against this side s wall surface of the camera hole Two screws 3 Connect the camera cable and USB cable Camera cable O 4 Proceed to install the lens See Section 3 5 3 Installing the lens and manual filter changer on page 34 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 33 3 Installation 3 5 Installation of hardware 3 5 2 Connecting the camera head to the computer 3 5 2 Connecting the camera head to the computer Connect the USB cable from the USB connector of the ImageQuant LAS 4000 mini camera head to one of the USB ports of the computer TZ Analysis PC ImageQuant LAS 4000 mini Note Use the USB cable supplied with the equipment Do not connect via a USB hub Do not use other USB equipment on the PC while ImageQuant LAS 4000 mini is in use This may result in the loss of image data 3 5 3 Installing the lens and manual filter changer The LAS High Sens lens is connected to the camera head as follows 1 Endthe ImageQuant LAS 4000 mini Control Software if it is running 2 Turn off the instrument 3 Loosenthe Epilight source angle ad
63. tton 3 4 2 3 Installation of ImageQuant LAS 4000 mini Control Software Windows VISTA 1 2 3 4 Insert the ImageQuant LAS 4000 mini Control Software CD Locate and double click the file ImageQuant LAS 4000 msi In the ImageQuant LAS 4000 InstallShield Wizard dialog click the Next button Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative see the back cover of this manual for contact information Select I accept the terms in the license agreement and click the Next button Select destination folder in the dialog JE ImageQuant LAS 4000 InstallShield Wizard Destination Folder Click Next to install to this Folder or click Change to install to a different Folder Install ImageQuant LAS 4000 to C Program Files T Change J installshield lt Back next Cancel Click the Next button to install the software at the default folder C Program Files Clickthe Change button to install to a different folder Click the Install button If User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified programs wants access to your computer Click Allow Click the Finish button The installation of ImageQuant LAS 4000 mini Control Software is now completed 5 5 Installation of hardware This section details the assembly that is required in order to use the ImageQuant LAS 4000 mini 32 Get
64. tton CU f WN N n Select Add a new hardware device and click the Next button Add Hardware Wizard The following hardware is already installed on your computer as S From the list below select an installed hardware device then click Next to check properties or troubleshoot a problem you might be having To add hardware not shown in the list click Add a new hardware device Installed hardware USB Root Hub C USB Mass Storage Device Generic USB Hub USB Composite Device S Generic volume lt Back Nest Cancel 6 Select Install the hardware that manually select from a list Advanced and click the Next button 26 Getting Started with ImageQuant LAS 4000 mini 28 9607 61 AB 7 Select Show All Devices and click the Next button Add Hardware Wizard From the list below select the type of hardware you are installing If you do not see the hardware category you want click Show All Devices Common hardware types Show All Des 3 Display adapt ices ters Fuifilm USB Device EIDE ATA ATAPI controllers amp IEEE 1394 Bus host controllers SB Imaging devices A Infrared devices Intel AIM Codec LB Madame Back Next gt Cancel 8 Click the Have Disk button 9 Insert the ImageQuant LAS 4000 CD and click the Browse button 10 Select to install the driver from the ImageQuant LAS 4000 CD Lock rc 5 Imagetuset LAS 4000 ID v Od 3 My Necert Documents gue

ImageQuant™ LAS 4000 mini - GE Healthcare Life Sciences

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ImageQuant&trade; LAS 4000 mini - GE Healthcare Life Sciences

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ImageQuant™ LAS 4000 mini - GE Healthcare Life Sciences