Cyclops 7 User Manual
Contents
1. 4 If the sample signal is high gt 3 0 V for example you may choose to use the X1 gain instead of the X10 gain setting so that you avoid going over scale once you deploy the CYCLOPS 7 5 If the sample signal is very low lt 0 3V you may choose to use the X100 gain setting to achieve higher sensitivity but a smaller measurable range This process is even easier for dye tracing applications Simply create the dye dilution of interest and record what signal level it provides on the three gain settings Once the appropriate gain setting has been determined order an integration cable for that particular gain See Appendix D for more information on integration cables Turbidity Cyclops Introduction The Turbidity Cyclops measures turbidity using an 850nm light source and detection of scattered light at a 90 degree angle which is similar to many modern day benchtop turbidimeters This unit provides a quick and accurate way to determine in situ turbidity eliminating the collection and storage of samples and minimizing the potential error associated with sample handling and processing Calibration Calibrating the Turbidity CYCLOPS is a simple process which requires the use of calibration standards Turner Designs recommends purchasing Amco Clear Analytical Turbidity Standards for non ratio instruments because these standards are non toxic safe solutions consisting mainly of de ionized water that comes prepared in a broad range of concen2. To drive them low the voltage should be 1 Volt maximum 0 Volts minimum You may need to connect the CYCLOPS 7 analog ground to the DCS ground Output type 2 Open Collector Signals This type of output is either open or connected to ground Connect the CYCLOPS 7 Gain Control pins directly to these outputs You may need to connect the CYCLOPS 7 analog ground to the DCS ground Output type 3 Relays CYCLOPS 7 User s Manual Page 19 TURNER Ss DESIGNS Relays act as a controllable switch Connect one end of the relay to the CYCLOPS 7 analog ground Connect the other end of the relay to the CYCLOPS 7 Gain Control pin CYCLOPS 7 User s Manual Page 20 Index A Analog Ground osent Sp 29 Analog Output voltage Analog Output voltage D Descrip ON ersinnen un RE Reuter E Emission Wavelength Excitation Wavelength G Gain switching signal 1 Gain switching signal 2 I MEV ene L Licni SOS EE linear range 2aicanscasntscndeeneanceasageccnareaeoewecradsoanenante SIRS e M Minimum Detection Limit CYCLOPS 7 User s Manual aeeeeeeeeeeeeeeeeeeeeeeeeeeeee TURNER Ss DESIGNS O optical WEIEN 12 Out of Warranty Service 14 P POW et DE eee 15 PSU Ground Connection 16 PSU Positive Connection 16 R E rr a tecsatee tet 12 S Signal Out to data logger esesseeeseeseseseeseesse 16 Solid EE E h
3. host end of cable 5 Pin out from Host End to CYCLOPS end or advise a Supply Voltage from Host is carried on pin b Supply Ground from Host is carried on pin c Analog Signal to Host is carried on pin d Analog Ground to Host is carried on pin Provide sufficient information for Impulse Enterprise to make the proper electrical hook up between the two connector ends 6 No of Pigtails required Provide contact details if different from your information entered in the Impulse Enterprises fields on their Contact Us e mail form For assistance from Turner Designs call 1 877 316 8049 For assistance from Impulse Enterprise call 1 800 327 097 CYCLOPS 7 User s Manual Page 18 TURNER Ess DESIGNS Appendix E Controlling the Gain of CYCLOPS 7 The operating range can either be set to one of the 3 available ranges which will be referred to as Static Gain Control or it can be dynamically changed to achieve the optimum operating range referred to below as Dynamic Gain Control The first approach is applicable to using the CYCLOPS 7 as a stand alone sensor The second approach is applicable to when the CYCLOPS 7 is integrated into a system with control capability Both methods are implemented by grounding or driving Low the appropriate Gain Control pin The X10 and X100 Gain Control pins are normally in a 1 or High state if they are not connected to anything This means the CYCLOPS default is to be in the X1 larges
4. NS Appendix A CYCLOPS 7 Specifications Parameter Specification 0 03 ug L Chlorophyll a Minimum Detection Limit 0 04 ppb Rhodamine WT 150 cells mL Cyanobacteria 0 04 NTU Turbidity Dynamic Range X1 0 500 ug L Chl a Depends on gain setting X10 0 50 ug L Chl a X100 0 5 ug L Chl a X1 O 1 000 ppb RWT X10 O 100 ppb RWT X100 0 10 ppb RWT X1 0 2 000 000 cells mL PC or PE X10 0 200 000 cells mL PC or PE X 100 0 20 000 cells mL PC or PE X1 O 3000 NTU Turbidity X10 O 1000 NTU Turbidity X100 0 100 NTU Turbidity 5V Max 265 mW LE Seo LE o oOoOoOOOoO Ambient 0 to 50 C Water Temp 2 to 50 C Light Source Source Light Emitting Diode Excitation a e Chl 460 nm RWT 550 nm PC 595 nm PE 528 nm Turbidity 850 nm Emission Wavelength Chl 620 715 nm RWT 590 715nm PC 670 nm PE 573 nm Turbidity 850 nm g Detection Wavelength 300 1 100 nm kee Dimensions length excludes connector Length 4 3 10 9cm Diameter 0 875 2 22cm Depth Rating Rating 600 meters meters Housing ee ee Stainless Steel CYCLOPS 7 User s Manual Page 15 TURNER DESIGNS Appendix B CYCLOPS 7 Wiring Table we Wire Supply Voltage PSU Positive Connection 3 15 VDC Black Supply Ground OVDC PSU Ground Connection a nu Out to data logger Multimeter Positive 0 SVDC Connection Green Analog Ground 0 VDC Multimete
5. PLATE FOR NON STANDARD PIGTAIL CABLE LENGTH CONNECTOR FOR CSM OO ES ere 18 CYCLOPS 7 User s Manual Page 2 TURNER DESIGNS ER e E 19 METHOD 1 STATIC GAIN CONTROL ENNEN 19 METHOD 2 DYNAMIC GAIN CONTROL sccccsssscsccccccccccsssssssssvsscsccececccccssensnscsssscsccscsccscossensnscssssescosoesess 19 INDE CR TT 21 Introduction Description The Turner Designs CYCLOPS 7 Submersible Fluorometer Turbidimeter is an accurate single channel detector that can be used for many different applications This instrument can be designed to detect pigment fluorescence dye fluorescence for dye tracing applications fluorescence of dissolved organic matter or be used as a turbidimeter It is intended to be integrated into a multi parameter system to obtain its power and to deliver an output voltage to the system data logger which is proportional to the concentration of the fluorophore particle or compound of interest When calibrated with a standard of known concentration the CYCLOPS 7 output voltage can be correlated to provide data of the actual concentration of the target fluorophore Inspection and Setup The CYCLOPS 7 shipment package consists of e CYCLOPS 7 Analog Detector P No 2100 000 configured and factory scaled for the specified analysis see identification letter stamped on the connector for specified analysis C Chlorophyll R Rhodamine F Fluorescein P Phycocyanin E Ph
6. TURNER Ss DESIGNS CN CYCLOPS 7 Submersible Sensors User s Manual Version 1 6 April 25 2007 P N 998 2100 TURNER DESIGNS 845 W Maude Ave Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0 TURNER Ss DESIGNS Table of Contents TABLE OR CON TENTS E Z IWITRODUCTIO IN 3 DsCRIPTION 3 Ier 3 EIERE 4 MEASUREMENTS WITH CYOLOPS 7 E 5 e 5 SEENEN 5 GAIN DETERMINATION PROCEDURE sn 5 TURBIDITY CES E 6 RO OAN O pee a en 6 ENEE 6 RECOMMENDED MEASUREMENT PRACTICES FOR USING YOUR CYCLOPS 7 IN TAE RD a a a a ee em ee ae td ne nn 8 USING THE SECONDARY STANDARD OPTIONAL ACCESSORY he 9 USE OF THE SOLID SECONDARY STANDARD FOR IN VIVO CHLOROPHYLL APPLICATIONS 10 USE OF THE SOLID SECONDARY STANDARD FOR DYE TRACING APPLICATIONS rires 11 RECOMMENDED MEASUREMENT PRACTICES u ccscsscsssscsccssssccccscsscccccsssscccsscssccccccsssccssssssces 11 LINEAR RANGE AND OUENCHTING st ttttetesssstttttetssstttttesssrtreetesssreree 11 TEMPERATURE CONSIDERATIONS a ne 12 CARE AND MAINTENANCE EE 12 EE 12 EEN 12 MARRAN UY E 13 E scat EEE coco cheese tect E A NA A eects 13 E 13 OUT OF WARRANTY SERVICE aan sat an nn nee ins 14 APPENDEXR Arena AT canne ne ces ase end tes esc ne ane asc s emo deeeeteenir et 15 CYCLOPS 7 SPECIFICATIONS EE 15 APPENDD EE 16 CYCLOPS 7 WIRING TABLE 16 GAIN SWITCHING TABLE EE 16 APELADA GE 17 CYCLOPS 7 PIGTAIL CABLE AND CONNECTOR INFORMATION ns 17 KRITIKEN 18 REQUEST FOR QUOTE TEM
7. YCLOPS is between 5 and 15 volts CYCLOPS 7 User s Manual Page 17 TURNER Ss DESIGNS Appendix D Request for Quote Template For Non Standard Pigtail Cable Length Connector For Use With CYCLOPS 7 To request a quote from Impulse Enterprise for a custom pigtail cable connector follow these steps D Complete the 6 lines below between the asterisks with your specific information IT Highlight the lines between the asterisks and copy to the PC clipboard HI Paste the information into the Comments field on the Contact Us page on the Impulse Enterprise web page at the following URL http www impulse ent com inquiry html IV Complete the remaining fields of the Impulse Enterprise lt Contact Us gt e mail form V Click on Send E Mail K KEKE K K K KE K KE KE K E E K K K K K K K K K K K K K K K K K K K K K K K K K K K K K K K K K K K K Please quote for a custom pigtail cable connector for use with Turner Designs CYCLOPS 7 fluorometer to meet the following requirements 1 Pigtail is required for integration with Identify multi parameter system as appropriate 2 Cable Length to include connectors see Page 15 for max length State units feet meters etc 3 Configured for XI X10 X100 gain range on CYCLOPS 7 Put a checkmark against desired gain 4 Required connector on non CYCLOPS end of pigtail Provide sufficient information for Impulse Enterprise to request a quote for the required
8. e the same output voltage from the sensor as in step 1 turning the Secondary Standard adjustment screw clockwise produces a lower signal 3 Next perform a chlorophyll extraction using a Laboratory Fluorometer Spectrophotometer or HPLC to determine the actual chlorophyll a concentration in the sample This will provide the correlation of actual chlorophyll a concentration for the sensor output voltage at that given gain setting 4 Now at any time the Solid Standard can be used to check establish a new correlation between a known equivalent concentration and the current l Information on doing a chlorophyll extraction can be found on the Turner Designs web site at this URL http www turnerdesigns com t2 doc appnotes 998_9000 html CYCLOPS 7 User s Manual Page 10 TURNER DESIGNS CYCLOPS 7 output voltage CYCLOPS 7 must be on the same gain setting as in Step 1 Use of the Solid Secondary Standard for Dye Tracing Applications The Solid Secondary Standard accessory can also be used to check the fluorescence stability for making dye concentration measurements If necessary the Solid Standard can be used to establish a new correlation voltage without the need to use a calibration solution each time l To use the Solid Standard to establish a correlation between a known dye concentration and the fluorescence output voltage immerse the sensor in a dye solution of known concentration say 50 ppb and note the sensor ou
9. ein 3 Supply Ground rte ssl 16 Supply Voltage sise 16 T Temperature ENEE beueen 15 temperature sensitive 12 AU UTS RIEL PE RER 6 W DO a a es 13 Warranty SOL VIC Len eg 13 Page 21 TURNER Ss DESIGNS Turner Designs 845 W Maude Ave Sunnyvale CA 94085 Phone 408 749 0994 Toll Free 877 316 8049 Copyright 2003 2004 Turner Designs P No 998 2100 CYCLOPS 7 User s Manual Page 22
10. es the output voltage to increase CYCLOPS 7 User s Manual Page 4 TURNER DESIGNS Measurements with CYCLOPS 7 Introduction The following procedure will describe how to make measurements with the CYCLOPS 7 Also it will describe how to use the accessory Solid Secondary Standard to make absolute or quantitative measurements Note that to make accurate and repeatable measurements it is important to keep the sensor clean see page 12 for information on cleaning your sensor Setting the Gain The gain setting refers to the sensitivity adjustment of the sensor There are three gain settings X1 X10 and X100 As the gain increases the sensitivity increases and the concentration range decreases All three gain settings can be used however you must determine which gain to use prior to deployment and have an integration cable made to activate a specific gain see Appendix B In other words only one gain setting can be used at a time this is referred to as Static Gain Control If you want the ability to utilize all three gains with a multi parameter system such as a CTD you will need to purchase three separate integration cables In most instances the X10 gain will provide the appropriate sensitivity and range If you are working in very low concentration applications lt 2 ug L chl a or lt 5 ppb rhodamine WT the X100 gain is recommended and if very high concentrations are expected gt 40 ug L chl a or gt 80 ppb rh
11. ge the signal level use the green screwdriver provided and insert the blade through the hole in the locking nut Rotate it until it engages with the CYCLOPS 7 User s Manual Page 9 TURNER DESIGNS adjustment screw that is beneath the locking nut Now the screw can be used to adjust the signal level as necessary Figure 4 Insert the supplied green screwdriver through the Adjustment screw d calibration hole in the locking is located under the locking nut nut to reach the adjustment screw 6 Once the desired reading has been obtained the locking nut should be screwed down so that the adjustment screw is held firmly in place 7 Finish by noting the output voltage and gain setting used X1 X10 or X100 in the Value space on the Secondary Standard label 8 Note that the response of every solid standard is unique Hence a new correlation must be determined for every sensor For future identification use the ID space on the label for a unique identifier for the Secondary Standard Use of the Solid Secondary Standard for In Vivo Chlorophyll Applications 1 To establish a correlation between a known chlorophyll concentration and the fluorescence output voltage immerse the sensor in a sample containing algae and note the sensor output voltage and gain setting used x1 x10 or x100 2 Dry off the CYCLOPS 7 attach the Solid Standard with the same gain setting and adjust the Solid Standard to produc
12. hould not come in contact with any organic solvents ie acetone methanol or strong acids and bases The UV Cyclops 7 models are the ONLY Cyclops 7 sensors that can be calibrated with Quinine Sulfate standards made in Hydrosulfuric Acid All other Cyclops 7 models CAN NOT be used in Hydrosulfuric Acid CYCLOPS 7 User s Manual Page 12 TURNER DESIGNS Warranty Terms Turner Designs warrants the CYCLOPS 7 Fluorometer Turbidimeter and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner Designs with the following restrictions The instrument and accessories must be installed powered and operated in compliance with the directions in this CYCLOPS 7 User s Manual and directions accompanying the accessories Damage incurred in shipping is not covered Warranty Service To obtain service during the warranty period the owner shall take the following steps Write or call the Turner Designs Technical Support Department and describe as precisely as possible the nature of the problem Phone 1 877 316 8049 E Mail support turnerdesigns com Carry out minor adjustments or tests as suggested by the Technical Support Department If proper performance is not obtained ship the instrument prepaid to Turner Designs with a statement of shipping charges The instrument will be repaired and returned free of charge along w
13. ith a check to cover shipping charges for all customers in the contiguous continental United States For customers outside of the contiguous continental United States and who have purchased our equipment from one of our authorized distributors contact the distributor If you have purchased direct contact us We will repair the instrument at no charge shipment documentation etc charges will be billed at cost to the customer NOTE The instrument or accessories should not be returned without first contacting Turner Designs Prior correspondence including an RMA number is needed a To ensure that the problem is not a customer solvable one easily handled in your laboratory saving the end user time and money b To specifically determine the nature of the problem so that repair can be rapid with particular attention paid to the defect you have noted CYCLOPS 7 User s Manual Page 13 TURNER DESIGNS Out of Warranty Service Proceed exactly as for Warranty Service above If our service department can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a fixed price basis plus any applicable duties and or taxes Shipment to Turner Designs should be prepaid Your bill will include return shipment freight charges Address for Shipment Turner Designs S45 W Maude Ave Sunnyvale CA 94085 Phone 408 749 0994 CYCLOPS 7 User s Manual Page 14 TURNER SS DESIG
14. lops does not have a solid secondary standard The two main benefits of using the Solid Secondary Standard are 1 It can be used in place of a primary liquid standard once a correlation between a primary standard and the solid standard has been established 2 It can be used to check the stability of the instrument and or check for loss in sensitivity resulting from the growth of bio fouling organisms on the sensor optics The Solid Secondary Standard provides a very stable fluorescent signal It has an adjustment screw so that you can tune the Solid Standard to provide a signal to match a specific sample Installing the Secondary Standard 1 Before installing the Solid Standard you must ensure that the optical surface of the CYCLOPS 7 is completely clean and dry The Solid Standard is indexed and it must be installed so that the indexing is precisely aligned for proper use see Figure 3 2 To install place the Solid Standard on to the optical end of the CYCLOPS 7 3 With the Solid Standard fully pressed on rotate in either direction until you feel the CYCLOPS 7 Fj 3 Solid Standard wure 3 indexing mark Align the index mark and indexing ball when installing CYCLOPS 7 in the Solid Secondary Standard Solid Standard indexing ball Solid Standard indexing ball click into the indexing mark on the CYCLOPS 7 4 To begin use a flat head screwdriver to unscrew the locking nut as far as it will go 5 Next to chan
15. mple 1 1 or some other convenient ratio If the sample is still in the linear range the reading will decrease in direct proportion to the dilution If the reading does not decrease in direct proportion to the dilution or 1f the reading increases the sample is beyond the linear range CYCLOPS 7 User s Manual Page 11 TURNER Ss DESIGNS 1 Fluorometer Response Curve K Sample Quenching Region 7 Figure 5 Graph showing Linear and Quenching Regions of the sample s response Sample Concen Temperature Considerations Fluorescence 1s temperature sensitive As the temperature of the sample increases the fluorescence decreases For greatest accuracy record the sample temperature and correct the sensor output for changes in temperature For further information on how temperature light water quality and the physiological state of the algal cells can all affect the measurement of chlorophyll a please refer to the application section of Turner Designs web site at the following URL http www turnerdesigns com esupport understanding html Care and Maintenance Rinsing The CYCLOPS 7 should be rinsed or soaked in freshwater following each deployment ideally until it is completely clean again Cleaning the Optics The optical window should be visually inspected after each deployment following a soaking in fresh water If cleaning is needed use optical tissue to clean the window with soapy water Note The CYCLOPS s
16. mprove the accuracy and repeatability of your measurements especially at low concentration levels l Use a Glass Beaker for your water samples Avoid plastic beakers plastic fluoresces and will interfere with the sample fluorescence Place the glass beaker on a Non Reflective Surface preferably black Ensure that the sensor is more than 3 inches above the bottom of the glass beaker Ensure that the sensor is in the center of the glass beaker and has more than 2 inches clearance between the cirumference of the sensor and the inside surface of the beaker Turner Designs recommends using a IL Glass Beaker for measurements with CYCLOPS 7 units Check that the optical surface of the sensor is free of air bubbles Be sure your sensor is calibrated see page 9 for Calibration Procedure To maximize consistency between measurements place sensor at exactly the same height for each sample This is most easily done using a Lab Stand Calibrated Sensor gt 2 inches all round No Air Bubbles On Optical Surface Dark Black Non Reflective Surface Figure 2 Summary of good measurement practices for using a submersible fluorometer in the lab CYCLOPS 7 User s Manual Page 8 TURNER DESIGNS Using the Secondary Standard Optional Accessory The following information will describe how to use the Solid Secondary Standard P No 2100 900 with CYCLOPS 7 sensors Note The Turbidity Cyc
17. odamine WT the X1 gain is recommended If you are uncertain of which gain setting to use you can take readings of a representative sample of water in the laboratory and determine which gain is the most appropriate Customers wanting to dynamically change the gain ranges to achieve the optimum operating range should refer to Method 2 Dynamic Gain Control in Appendix E for how to interface with a Data Collection System with programmable outputs Gain Determination Procedure 1 For in vivo chlorophyll applications take a natural sample of water from a sampling station where you plan to deploy the CYCLOPS 7 Applying good measurement practices store it properly and quickly transport it to a laboratory where you have the CYCLOPS 7 connected to a multimeter and DC power source see Figure 1 2 Pour the water sample into to a clean glass beaker and submerge the optical end of the CYCLOPS 7 See the section on Recommended Measurement Practices for using your CYCLOPS 7 in the Lab for how best to accomplish these steps 3 Activate the X10 gain setting see Wiring Table on page 16 if you believe the sample to represent a typical condition 1 e not a bloom or other abnormal event You would like to obtain a signal from the sample that is significantly higher than a blank sample De ionized water or filtered seawater but not a signal that is close to the maximum of 5 Volts CYCLOPS 7 User s Manual Page 5 TURNER DESIGNS
18. r Negative Connection Blue 5 X10 Gain Medium See table below Sensitivity Brown X100 Gain High See table below Sensitivity Gain Switching Table Gain 100 l Chl Range Brown ug L TT Connected to 0 50 0 100 0 1000 Not connected X 10 analog ground analog ground CYCLOPS 7 User s Manual Page 16 TURNER Ss DESIGNS Appendix C CYCLOPS 7 Pigtail Cable and Connector Information Figure 7 Dimension details of 24 length cable with 20 gauge colored leadwire connects to 6 Figure 8 Female locking sleeve Impulse P No MCDLS F ry Lk LE Se NEOPRENE Sp dE GE ie La Vi LZ SC dv al OCH d SS Ly L i 2 9 MATED cual Figure 9 In line connector contact configuration connects to CYCLOPS 7 NEOPRENE 6 CONTACT See Appendix D for a Quote Request D SE d BREE 2 014 template to order a non standard pigtail ETS cable length connector for use with the X TEFLON BRASS fo i STAINLESS CYCLOPS 7 Fluorometer STEEL pin male connector Cable manufacturer Part No IMPULSE MCIL 6 FS A Maximum cable length up to 300 meters can be connected to the CYCLOPS if the following conditions are met 1 The cable is shielded and contains 20 gauge conductor size or greater 1e Belden No 8426 cable 2 The 0 5 volt Analog output is connected to a device 1e Data Logger with an input impedance of 1 MegOhm or greater 3 The supply Voltage to the C
19. t concentration range mode The CYCLOPS 7 can be put into higher gain lower concentration range modes by connecting either the X10 or X100 pin but not both at the same time to ground Method 1 Static Gain Control Connect the X10 or X100 pin to the analog ground pin of the CYCLOPS 7 pigtail connector See the Gain Switching Table in Appendix B to determine the required configuration for desired gain range measurement range See Appendix C for Pigtail Cable and Connector information Also see Setting the Fluorometer Gain on page 5 for more information on Static Gain Control Method 2 Dynamic Gain Control If you have a Data Collection System DCS that has programmable outputs you can use them to control the CYCLOPS 7 gain signals Following are three common output types found in Data Collection Systems and how to connect them to the CYCLOPS 7 Refer to the manual of your DCS to determine which is appropriate For those who want the technical data the X10 and X100 Gain Control pins of the CYCLOPS 7 are connected internally to the input of a Schmitt trigger inverter part number 74LVC1G14 and a 100K ohm pull up resistor Both use a 5 Volt power supply Output type 1 Digital Signals Logic signals can be used to drive the Gain Control pins In most cases you can connect the digital signal output of the DCS directly to the CYCLOPS 7 Gain Control pins To drive them high the voltage should be 3 0 volts minimum 5 volts maximum
20. tput voltage and gain setting used Dry off the optical end of the CYCLOPS 7 attach the Solid and adjust to produce the same output voltage from the sensor as in step 1 turning the secondary standard adjustment screw clockwise produces a lower output Now at any time the Secondary Standard can be used to check establish a new correlation between a known equivalent concentration and the current CYCLOPS 7 output voltage Comprehensive information on dye trace measurements can be found at the following Turner Designs URL http www turnerdesigns com t2 doc appnotes main html fluorescent Note There is no Solid Secondary Standard available for Turbidity Cyclops Recommended Measurement Practices Linear Range and Quenching The linear range is the concentration range in which the CYCLOPS 7 output is directly proportional to the concentration of the signal The linear range begins with the smallest detectable concentration and spans to an upper limit concentration that is dependent upon the properties of the material filters used and path length A non linear relationship is seen at very high concentrations where the signal does not increase at a constant rate in comparison to the change in concentration see Figure 5 below At even higher concentrations the signal will decrease even though the sample concentrations are continuing to increase This effect is known as signal quenching Linearity can be checked by diluting a sa
21. trations and has a shelf life guaranteed for one year The Turbidity CYCLOPS can be calibrated using a single point calibration which correlates a known standard to the signal measured from a typical sample 1 Connect the CYCLOPS 7 to a power source and measure the turbidity of a typical water sample from the source being monitored recording the voltage output of the sample 2 Set the CYCLOPS 7 to the proper gain see Gain Setting for explanation on how to set the gain 3 Use turbidity standards of known concentrations NTU and create a correlation between the standard NTU and the voltage output volts Note make sure to use a Standard concentration that can be read on the same gain setting as typical samples See page 15 for typical NTU range at gains 1X 10X and 100X 4 Once correlation has been made use the following equation to calculate turbidity values for future measurements Sample NTU NTUsta Std voits Samplevoits NTU sta Concentration of Standard used for calibration CYCLOPS 7 User s Manual Page 6 TURNER Ss DESIGNS Std volts Voltage reading from known concentration of standard Sampleyoits Voltage reading from samples 5 Switching gains will require re calibration if the signal has exceeded the maximum voltage for a gain setting CYCLOPS 7 User s Manual Page 7 TURNER SS DESIGNS Recommended Measurement Practices for Using Your Cyclops 7 in the Lab The following steps will i
22. ycoerythrin U CDOM O Crude Oil B Optical Brighteners T Turbidity e CYCLOPS 7 Documentation Kit P No 2100 998 includes User s Manual on CD CD P No 005 2100 and Quick Start Guide e Calibration Certificate e Quick start Guide Flow Through Cap assembly instructions Secondary Standard Instructions and n Vivo Calibration Procedure all on above CD Optional Accessories include Cable Assy C7 Pigtail 2 Ft P No 2100 750 Solid Standard P No 2100 900 Additional Equipment required for functional tests DC Power Supply 3 15 VDC gt 100 mA Multimeter to read 0 5 VDC CYCLOPS 7 User s Manual Page 3 TURNER DESIGNS Functional Test To perform a functional check on the CYCLOPS 7 connect the interface colored wires to the power supply and multimeter as shown in Figure 1 Note Supply voltages greater than 15 VDC will result in damage to the sensor DC Power Supply Multimeter PSU Positive Supply Ground Connection Red 0 VDC Black Signal Output CYCLOPS 7 White Fluorometer Ground Green X10 Gain Blue X100 Gain Brown Figure 1 CYCLOPS 7 connections fi unctional Leave both wires disconnected for Functional Check X1 Low Gain With the CYCLOPS 7 connected as shown in Figure 1 above make the following functional checks The LED is on The multimeter reads gt 0 VDC Moving the light source close to your hand caus
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