Manual CAR V5 Feb11
Contents
1. Y R Y Y Y Y W s Tt Y a ay b d R Y Y Y i d AD p P F TUY 2 a D d 5 d d Sg ae 4 q b ae TY a wa 4 d _ L SN hem Sim r Yy 1 Q E BS J h amp A aa pm E owe we amp ie i amp Y a A Y Y amp EAN E T S if i Edit Edit Edit Edit Edit Edit Edit Edit Edit Edit Edit Edit il i il jil il il jil il il i r w 4 Set Assay IDs Set Sample IDs Cancel Next Stop Import Set Sample IDs Cancel Next Fig 4 Left Set Assay ID option on the lower left side of the screen Right All strips turn unselected when chosen this option Every barcode read a strip is activated automatically on the microplate sketch and colour combination turns from grey into a specific assay s colour combination Fig 5 Select Rack 16 Well Plate 0 0 0 0 0 0 0 0 a o E am pie ee bh A AN a a ey I p gt di dih dih di a L SS ke 5 a dih di 1 L ae bd k E 4 A D d al lt e lt p N d J h q pam ee gt d ia q Edit Edit Edit Edit Edit Edi Edi Edi Edit Edit Edit Stop Import Set Sample IDs Cancel Next gt gt Clinical Array reader CAR Manual Version 5 April 2011 15 Clinical Array Reader CAR User Manual GEN MICA Fig 5 Specific colours combinations Red HPV Blue Pneumovir Green Entherpex Manual setting Software s2. Q Q DSD Ef Sh ct S S Fig 17 Selecting Image option Fig 18 Example of a HPV assay performed in strips Clinical Array reader CAR Manual Version 5 April 2011 24 Clinical Array Reader CAR User Manual GEN MICA By selecting Raw Data option user accesses to the numerical values measured for each virus type Fig 19 and 20 Virus Virus Result Controls Export Export Type 6 Type 11 s Type 16 Negative Passed Erini Pae Type 18 Negative Passed Type 26 Negative Passed Image Image Type 31 Passe Type 33 Passed Type 35 Negative Type 40 Passe Type 42 Negative Passed pag 0 0068 Type 43 Negative Passed rey Type 44 Negative Passed Type 45 Tima E9 lt lt Back _Rewata_ Cs Fig 19 Selecting Raw Data option Fig 20 Raw data and normalized values 8 7 REANALYZING SAMPLES After the analysis process red coloured wells where the grid alignment has failed can be reanalyze manually by user In the microplate sketch menu Fig 15 press on a red coloured sample position to activate the reanalysis main menu window Browse the proper Assay among those from the pull down menu Fig 21 and press Start Reanalyze Reanalyzation Information Select Assay CLART_HPVZ x CLART_HPV2_MM CLART_HPV2 Pneumovir lt lt Back Start Reanalyze Fig 21 Main m
3. Select Rack 96 well plate J o o o o o o 0 o o o 0 o Jaee Set Assay IDs Set Sample IDs Fig 3 96 wells microplate sketch on the New Analysis menu This menu Fig 3 represents the cornerstone of the application since it allows the user to Set rack type choose between 96 wells microtitter plate AS CS or tubes adaptor AT Set the sample s number to be processed Set assay ID HPV2 PneumoVir ENTHERPEX EnteroBactv Set sample ID Sample Reference Clinical Array reader CAR Manual Version 5 April 2011 14 Clinical Array Reader CAR User Manual GEN MICA 8 1 SETTING SAMPLE S NUMBER The number of samples to be analyzed can be introduced manually or automatically using a barcode reader It s not necessary to place the AS CSs in correlative positions for their analysis Nevertheless when the CAR is included in a Clinical Array Processor CAP AS CSs must be placed in correlative order at the left side of the frame Automatic setting For the automatic setting of sample s number user will need a bar code reader connected to the CAR normal USB connection See FAQ 2 GENOMICA can provide the user with specific bar codes for each assay upon request Press Set Assay IDs option placed on the lower left side of the screen and all strips will turn unselected on the New Analysis menu Fig 4 SelectRack 96 wel piate W SelectRrack 05 wel pate 0
4. GEnemicA Archive Shut Down Fig 78 Select Settings options on the secondary menu Clinical Array reader CAR Manual Version 5 April 2011 54 Clinical Array Reader CAR User Manual Backup GEN MICA Reader Menu ra Q Fig 79 Browse directories by using the option and select directory which corresponds to the USB stick Keep in mind that directory assigned to USB may be subjected to chages System Information Date Time 04 07 2008 09 26 19 Export Path C Genomica Data CARExport e Backup Path C Genomica data Backup a Language English J Fig 80 Once selected the export path all exporting data will be send using the export path Data exporting should be performed from the Archive menu following the next protocol Select Archive option from the Main Menu Clinical Array reader CAR Manual Version 5 April 2011 55 Clinical Array Reader CAR User Manual GEN MICA New Analysis Service GEN MICA Shut Down Fig 81 Once selected this option a searching engine will pop up On this new window it s possible to restrict the searching time period inserting a start searching date and end searching date Select Start End Date Start Date 2007 09 15 O End Date Year Month Day lt lt Back Next gt gt Fig 82 Search engine will find out all results contained between the two indicated d
5. This symbol indicates the risk of loss of life or severe injury to the user due to high voltage This symbol indicates important information about the CAR and analyzing process Please read these instructions carefully gt oo This symbol indicates important information regarding ATs analyse UA Ye Clinical Array reader CAR Manual Version 5 April 2011 59
6. 11 4 DONGLE WHAT IT IS AND ITS USE Dongle is the name of an electronic key with USB appearance Depending on the CAR s serial number Dongle can be found whether the rear side of the CAR attached with a bridle or internally not visible When the Dongle is placed outside in the rear side just insert the Dongle in a free USB port in order to let the CAR work Without the Dongle CAR will start a maximun of 30 times before stop working When the Dongle is placed inside the CAR it will be indicated in a sticker at the rear side Dongle works as a software license In case of Dongle failure GENOMICA application won t be launch and CAR won t be operative Please do not remove the Dongle from the CAR unless for shipping it to technical service Dongle key Clinical Array reader CAR Manual Version 5 April 2011 58 Clinical Array Reader CAR User Manual GEN MICA 12 APPENDIX 1 ABBREVIATIONS USED AS Array Strips AT Array Tube CAR Clinical Array Reader Fig Figure PCR Polimerase Chain Reaction PDF Portable Document Format generate by Adobe LIMS Laboratory Information Management System CLART Clinical Array Technology 13 APPENDIX 2 SYMBOLS AND WARNING SIGNS Yellow triangles indicate general warnings Warnings include the risk injury to the user or serious equipment damage This symbol indicates severe injury to the instrument operator due to interference of the user with moving parts of the CAR
7. Fy em ea ZEE SPACE BAR Copy Paste P m ie X R Q W A Fig 9 Setting Samples IDs manually using the tactile keyboard The user can check the sample position at any time since it appears at the upper left side of the screen Once finished with samples identification process press OK to go back to the New Analysis menu Fig 2 Clinical Array reader CAR Manual Version 5 April 2011 19 Clinical Array Reader CAR User Manual GEN MICA Then press NEXT 8 4 WORKING LIST The working list allows the user to check whether the already introduced information samples references assays is correct or not To change any data just press Back and repeat the setting process again see Set Samples IDs Manually and Set Assays ID Manually If all information is correct press Start to carry on with the reading process Fig 10 Gases SAU Working list CAR CAP System Rua ID 100002 Date and time Thu Jan 10 20 47 09 2008 GEN MICA Pos Assay Array ID kag Sample reference Result Al 40204 Papilloma 000000040204 1 654684 B1 40204 Papilloma 000000040204 2 16687 C1 40204 Papilloma 000000040204 3 4987 Di 40204 Papilloma 000000040204 4687 Ei 40204 Papilloma 000000040204 5 0136 F1 40204 Papilloma 000000040204 6 13786 be G1 4020
8. Selecting Settings option at the Service menu lt lt Back Press button System Information Date Time 04 07 2008 09 26 19 Adjust Export Path C Genomica Data CARExport Backup Path C Genomica data Backup sada Language English lt lt Back Fig 63 Box position Browse directories and external devices connected to the CAR and select the destination Fig 64 Press OK when finished D Fig 64 Selecting directories or external devices Clinical Array reader CAR Manual Version 5 April 2011 A6 Clinical Array Reader CAR User Manual GEN MICA Once set the backup path user can backup results by pressing the Backup button Fig 65 I Import System Backup O NG Reader Menu Fig 65 Selecting Backup option Next select Results Fig 66 on the touch screen and then Next Select Backup Type GEN MICA Results Configuration data lt lt Back Next gt gt Fig 66 Selecting Results backup To speed up data backup user can introduce a time interval All results reports included between the starting date and ending date will be back up Fig 67 Then press Next Clinical Array reader CAR Manual Version 5 April 2011 47 Clinical Array Reader CAR User Manual GEN MICA Select Backup Start End Date Start Date 2008 04 07 End Date Year Month D
9. lt lt Back Fig 43 Example of a HPV assay performed in strips By selecting Raw Data option user accesses to the numerical values measured for each virus type Fig 44 and 45 Virus Virus Result Controis Type 6 Negative Passed Type 11 Negative Type 16 Type 18 Negative Passed Type 26 Negative Passed Type 31 Negative Passed Type 33 Type 35 Type 38 Type 40 Negative Passed Type 43 Type 44 Type 45 Negative Passed Tima ED Raantiuin Nannnd Fig 44 Selecting Raw Data option gad vey 0 0078 mye 0 0081 Export 0 0012 0 0013 0 0011 0 0011 0 0089 Image Type 42 0 0036 0 0055 0 0037 Type 40 0 0095 0 0098 Negative Type 43 0 0027 0 0028 Negative Type 44 0 0010 0 0011 0 0058 Negative Negative 0 0053 Type 54 zN Raw Data a Fig 45 Raw data and normalized values 9 9 SAMPLE REANALYSIS 0 0049 0 0094 0 0051 Export Print Image GK After the analysis process red coloured wells where the grid alignment has failed can be reanalyze manually by the user In the tube adaptor sketch menu Fig 46 press on a red coloured sample position to activate the reanalysis main menu window Fig 47 Clinical Array reader CAR Manual Version 5 April 2011 38 Clinical Array Reader CAR User Manual GEN MICA Run Number 88 Pl
10. 24 Clinical Array reader CAR Manual Version 5 April 2011 26 Clinical Array Reader CAR User Manual GEN MICA PP Reanatyes ag Zoom x 7 INN 3 TE LAN o 1 G 3 a m e A ext a a Cpr GY a A 2 a Gy a a f T T x p T T p a G x AQ 2B G Q a te t gt an ab ah a y 3 Co 3 a gt d a 3 AQ Q m m Fig 24 Grid alignment checking A Manual grid alignment implies user s intervention in the results If a manual grid alignment is required an informative message will be shown in the run report The image has been reanalyzed by user Fig 25 Informative message indicating the manual reanalysis Clinical Array reader CAR Manual Version 5 April 2011 27 Clinical Array Reader CAR User Manual GEN MICA 9 PERFORMING TUBES 9 1 PLACING ARRAY TUBES ON THE TUBE ADAPTOR The ATs adaptor is provided by GENOMICA and allows the user to place up to 12 ATs for analyzing For the adequate analysis of the tubes is necessary to place them properly on the adaptor Open the AT tube completely and turn back its cap Hold the tube in this position and introduce both tube and cap at the same time into the adaptor as shown in Fig 25 Tubes will be read in columns like the ASs starting from position A1 which is placed on the blunted square of the tube adaptor up
11. Clinical Array reader CAR Manual Version 5 April 2011 53 Clinical Array Reader CAR User Manual GEN MICA E windows system3 cmd exe z olx Dospatch Genomicasscripts Sunreport error html D patch Genomica scripts runreport py Dospatch Genomicasscripts workinglist error htnal D spatch Genomicasscripts workinglist py D2 patchGenomicascripts working list tmo html DoNpatch Genomicasscripts working list voc 16G3 D patch Genomica scripts workinglist voc i634 38 Filets copied D gt patchssensovat ionSCLARI_HPUZ25120er with 5 CircleRefs syvary NispatehSSensouat tansCLART_HPU 8 lgorithm sualg Dospatch Sensovat ions CLART_HPU2 CLART_HPU2 svexp spatchSensovation Metabone 1leBer with 5 Girclelers svary Dospatch Sensovat ion MetaboneAlgorithn svalg Despatch sensovat ion Metabone Metabone svexp Nispate hs Sensouat tonsPhenmuipesi Afer with GS S amp quareRefs suary Dospatch Sensovat ion Poeumovir Algorithm svalg Despatch Sensovat ton Dnceumoyvir neumovir syvexp Filets copied CLART HFU2 install ok leass restart the reader for changes to take effect Fig 77 11 2 EXPORTING DATA EXPORT PATH User can select where to export all data Results can be exported to a USB stick an external hard disk or to the LIMS just by selecting the proper export path Steps for exporting results to an USB stick Press Service option of the Genomica s main menu New Analysis Service
12. GENOMICA application will appear on the touch screen EN MICA i Fig Secondary switcher at the front side 7 2 SWITCHING OFF First press Shut Down box on the main menu to switch off the CAR and then turn off the main switcher at the back side It is important to shut down the CAR when not required as this may lengthen its half life Fig 1 Do not switch off the reader directly since it could damage it Clinical Array reader CAR Manual Version 5 April 2011 12 Clinical Array Reader CAR User Manual GEN MICA New Analysis Service GEN MICA o Shut down option at the GENOMICA S main menu 8 READING STRIPS AS CSs properly positioned on the tray as it is shown in the image below In case of When performing CLART Strips make sure that the metallic frame required is performing Array Strips metallic frame is not necessary Reading and analyzing samples is a sequential easy procedure compose by multiple menus where user can choose among the several options of the application In order to start new analysis choose option New Analysis on the application s main menu Fig 1 Clinical Array reader CAR Manual Version 5 April 2011 13 Clinical Array Reader CAR User Manual GEnemicA GEN MICA Fig 2 Main menu of the GENOMICA s application Once this option is selected a new menu will pop up on the screen where 96 wells microplate sketch is depicted Fig 3
13. Tubes only Any other tubes vials or material must not be used with this equipment Only use Strips or Tubes processed in accordance to the AS CS AT protocols provided by GENOMICA Use of this equipment in a different way of that described in this manual will result in lost of your warranty rights Please consider that the USB used for software updates or results export does not contain any antivirus GENOMICA is not responsible about viral infections and CAR cleaning will not be considered as a part of the technical support Clinical Array reader CAR Manual Version 5 April 2011 11 Clinical Array Reader CAR User Manual GEN MICA 7 SWITCHING ON OFF THE CAR 7 1 SWITCHING ON Check if your CAR requires a Dongle key see FAQ 11 4 Depending on the CAR serial number dongle key might be already installed inside or should be installed by user A sticker on the rear side of the CAR will inform about the position of the dongle Plug in the reader and switch it on The main switcher is on the rear right side of the device Once pressed function gauge will display a yellow light Press then the secondary switcher Fig below on the front side of the CAR and wait until function s gauge turns green Once your CAR is switched on the instrument will initialise during this time the instrument will verify its good performance by a series of self tests After finishing this set of self tests the Main Menu screen
14. assays is correct or not To change any data just press Back and repeat the setting process again see Set Samples IDs Manually and Set Assays ID Manually If all information is correct press Start to carry on with the reading process Fig 36 _ Working list Print Genomica SAU Rev 14 CAR CAP System Run ID 100003 Date and time Wed Feb 25 09 54 28 2009 GEN MICA Wati C2 40207 CLART HPV2 MM 900000040207 16 Lv Operational Mode Process and Reading Fig 36 Workinglist when performing ATs 9 7 READING PROCESS Reader s tray opening and closing processes are performed automatically Do A not try to force or move it manually If neccessary press the emergency button placed on the rear side of the CAR User will be requested to initialize the process Fig 37 by pressing the Open Tray box Clinical Array reader CAR Manual Version 5 April 2011 34 Clinical Array Reader CAR User Manual GEN MICA GEN MICA Open Tray Fig 37 Message to initialize the reading process Once finished the opening process place the tubes adaptor ATs manually in the tray and operate the Close Tray box Fig 38 GEN MICA Close Tray Fig 38 Message to activate the closing process of reader s tray Reading procedure can be monitored in the screen since samples wells change their colours indicating the reading status Fig 39 Wells in green Samp
15. default settings establish the analysis of the whole microplate composed by up to 12 strips Every strip not included in the process must be manually unselected by the user To unselect them proceed to press on the symbol which will turn into Q indicating the no selection of the strip The unselected strip appears on a light grey colour Fig 6 SelectRack 96wellpate sy 00000000 ooo Y p 4s 6 7 Yi peaa DPE 11 x AL i h q Ah g SeN i f e d i a C d p od ae eS S a 7 y on ca AE JE JE i a ab ae h d gt amp Kij a b p SE YE YE YE JE YE bod h bod Eo T ce k 4 b 4 no ern SS nen C1 force e p Ae o aaa Set Assay IDs Set Sample IDs Oon Fig 6 Selection of AS number ay Sample s number should be always a whole multiple of eight which is the number of wells included in each strip 8 2 SETTING ASSAYS IDs The automatic setting of sample s number in the previous section allows the user to skip this step Setting Assays IDs Manually Software processes by default the CLART HPV2 assay To read or analyse any other GENOMICA S CLART Assay press the button on the New Analysis menu Fig 7a placed under each strip Option Edit activates a new window Fig 7b that lets the user choose among those assays shown in the pull down menu User can apply same Assay ID to the whole plate fro
16. menu Sample ID Search samples with same code or ID analyzed during the specified time period Assay ID Search samples processed with same procedure or assay during the specified time None Search for all samples included in the indicated time period Select Filters Sample ID Assay Name None lt lt Back Next gt gt CLA IL JPE JL Jere pe Te eg P ER SD Met sll co SPACE BAR Copy Paste 4 1 2 3 sur 2 x C Fig 54 Available searching parameters 10 2 SERVICE By operating the Service button Fig 55 user accedes to a submenu containing several reader s options Fig 56 New Analysis GEN MICA Archive Shut Down Fig 55 Selecting Service option from the Main Menu Clinical Array reader CAR Manual Version 5 April 2011 42 Clinical Array Reader CAR User Manual GEN MICA Import System Backup Reader Menu Fig 56 Multiple options of the Service submenu 10 2 1 IMPORT This option Fig 57 is mainly used for eventual updates in the software version See section 11 1 for extended explanations System Backup Reader Menu Fig 57 Selecting Import option at the Service Menu Clinical Array reader CAR Manual Version 5 April 2011 43 Clinical Array Reader CAR User Manual GEN MICA CA DA D Fig 58 Multiple importing so
17. 4 Papilloma G ars 40204 Papilloma H m 40204 Papilloma A2 40204 Papilloma B2 40204 Papilloma Working list Run ID 100002 Date and time Sat Feb 02 09 59 57 2008 Array ID Well nr 000000040204 1 1 000000040204 2 2 000000040204 3 3 000000040204 te 000000040204 5 5 000000040204 oO 000000040204 7 7 000000040204 000000040204 000000040204 wo D User Manual Result Genomic DNA control Passed Amplification control No signal Type 58 Genomic DNA control Passed Amplification control No signal Type 45 Genomic DNA control Passed Amplification control No signal Type 18 Type 8 Genomic DNA control Passed Amplification control No signal Type 33 Type 58 Genomic DNA control No signal Amplification control No signal Type 6 Type 11 Type 16 Type 51 Type 61 Type 84 Grid alignment failed The image could not be analysed Genomic DNA control No signal Amplification control Passed Type 5 Grid alignment failed The image could not be analysed Genomic DNA control Passed Amplification control No signal Type 33 Type 58 Genomic DNA control No signal Amplification control No signal Type 6 Type 11 Type 16 Type 51 Type 61 Tyne 84 GEN MICA GEN MICA Ey x Fig 14 Summary report created by the CAR at the end of the analyzing process RESULTS To visualize a specific sample s result select the sample posit
18. 4 Papilloma 000000040204 7 34684 H1 40204 Papilloma 000000040204 8 1 68789 A A2 40204 Papilloma 000000040204 9 1657345 B2 40204 Papilloma 000000040204 10 1678 C2 40204 Papilloma 000000040204 11 04797654 vV D2 40204 Papilloma 000000040204 12 996432175 E2 40204 Papilloma 000000040204 13 1657486 Operation Mode Process and Reading Reading Fig 10 Working list prior to analyzing The working list includes necessary information for the samples traceability references assay ID position on the microplate 8 5 READING PROCESS TAKING IMAGES Reader s tray opening and closing processes are automatic Do not try to force or move it manually If neccessary press the emergency button placed on the rear side of the CAR User will be requested to initialize the process Fig 11 by pressing the Open Tray box Clinical Array reader CAR Manual Version 5 April 2011 20 Clinical Array Reader CAR User Manual GEN MICA GEN MICA Open Tray Fig 11 Message to initialize the reading process Once finished the opening process place the microplate manually in the tray and operate the Close Tray box Fig 12 GEN MICA Close Tray Fig 12 Message to activate the closing process of reader s tray Reading procedure can be monitored in the screen since samples wells change their colour indicating the reading status Fig 13 Wells _in_gre
19. Clinical Array Reader CAR User Manual GEN MICA CUINICAL ARRAY READER CAR USER MANUAL m Clinical Array reader CAR Manual Version 5 April 2011 1 Clinical Array Reader CAR User Manual GEN MICA 1 TABLE OF CONTENTS 1 Table of contents 1 2 Introduction 4 3 Components Description and Specifications 5 4 Installation 7 4 1 Precautions 4 2 Unlock lLock for shipments 4 3 Assembling Screen 4 4 Connecting screen and reader 5 Safety Notes 11 6 Procedural Limitations 11 7 Switching on off 12 8 Performing Strips ASs 8 1 Setting Samples Number 14 8 2 Setting Assay IDs 17 8 3 Setting Sample IDs 18 8 4 Working List 20 8 5 Reading 20 8 6 Summary Report and Results 22 8 7 Sample Reanalysis 25 9 Performing ATs 9 1 Placing ATs on the Adaptor 28 9 2 Changing Rack Type 29 9 3 Setting Samples Number 30 9 4 Setting Assay IDs 31 9 5 Setting Sample IDs 32 9 6 Working List 34 9 7 Reading Process 34 9 8 Summary Report and Results 36 9 9 Sample Reanalysis 38 10 Main Menu Options 10 1 Archive 42 10 2 Service 43 10 2 1 Import 44 10 2 2 System 44 Clinical Array reader CAR Manual Version 5 April 2011 2 Clinical Array Reader CAR User Manual 10 2 3 Backup Backup Path 10 2 4 Settings 11 FAQ 11 1 Updating software 11 2 Exporting results Backup path 11 3 Barcode Reader Characteristics and Installation 11 4 Metal frame for CLART Strips Appendix 1 Abbreviations Used Appendix 2 Symbo
20. For the automatic setting of sample s number user will need a bar code reader connected to the CAR normal USB or PS 2 connection Once the adaptor type is changed see Changing Rack Type above press Set Assay IDs option on the lower left side of the screen and all positions will turn unselected Fig 29 and 30 Read as many barcodes as sample s number you have and tubes will turn to the specific assay s colour combination Fig 31 Select Rack v Set Plate ID Select Rack Set Manually Set Assay IDs Set Sample IDs Cancel Next gt gt Stop Import T Fig 29 and 30 Setting Assay IDs automatically Select Rack Set Manually o E o EO Stop Import Fig 31 Different colours combinations for each array type Clinical Array reader CAR Manual Version 5 April 2011 30 Clinical Array Reader CAR User Manual GEN MICA Manual setting In case of analyzing ATs those tubes that are not going to be processed should be unselected manually by pressing on g symbol The no selection of one tube will be indicated with x symbol and the tube position will turn into a light dark grey combination Fig 32 Select Rack Tube Adapter Set Plate ID Edit Assay Set Assay IDs Set Sample IDs Cancel Fig 32 Selected positions appearing in assay s specific combination Unselected positions turned into light dark grey combination 9 4 SETTING ASSAYS IDs The automatic setting of sample s num
21. PV2_MM CLART_HPV2 CLART_Pneumovir_13 Fig 69 Setting Default Assay Version 5 April 2011 49 Clinical Array Reader CAR User Manual GEN MICA 11 1 SOFTWARE UPDATING GENOMICA will provide the software necessary to read eventual updates or changes in the kits Please take care that the USB used does not contain any virus GENOMICA is not responsible about virus infections and CAR cleaning will not be consider as a part of the technical support Uncompress the zip file we have sent you on your pen drive Check that the pen drive now contains one folder called patch and one or more bat files with the name of the kit that you want to install It is important that all of them are in the main directory e g D and not included inside any folder Removable Disk D m E X File Edt wew Favorites Tools Help a 3 F Search IE Folders Address SE Di1 D el patch HPY 2_inskal Metabone i Proeurmowir_ins 22 objects 64 8 MB al My Computer Fig 70 Connect your pen drive to a free USB connection available on the back of the reader Clinical Array reader CAR Manual Version 5 April 2011 50 Clinical Array Reader CAR User Manual GEN MICA Fig 71 Go to the Service menu New Analysis Service GEN MICA Fig 72 Selecting Service option Press Import button Clinical Array reader CAR Manual Version 5 April 2011 51 C
22. ader CAR Manual Version 5 April 2011 7 Clinical Array Reader CAR User Manual CEN MICA Fig 1 Unlocking the CAR Position of the locking screws Place the white plastic cap attached in the hole left by the lock screw at the side of the CAR Fig 2 Fig 2 Covering the side hole left by the screw Should you need to send your instrument the lock screws must be screwed securely in their positions Unscrewed readers are susceptible of further damages during transportation To install the transport lock screws please follow these short instructions Turn off the instrument and remove the main power connection Slowly slide the tray holder manually fully towards the rear wall and place the screw at the rear of the CAR Then slide the tray holder fully to the right and place the screw on the side Tight them securely Clinical Array reader CAR Manual Version 5 April 2011 8 Clinical Array Reader CAR User Manual os GEN MICA 4 3 ASSEMBLING THE SCREEN Touch screen is provided separately In order to connect it please follow these indications 1 Unscrew partially the two lower screws yellow and totally those marked in red all of them are placed on the rear side of the reader 2 Lean the screen over the reader fitting the screws with the positions marked in yellow Insert the safety lock screws recently removed green circles below in the positions marked in red See below 3 Once
23. ate ID 100003 View Report O O Fig 46 Grid alignment failed positions when performing Pneumovir Kit When performing CLART HPV2 in tubes alignment must be carried out with the biotin markers instead of Metal Markers Protocol stays the same The correct grid alignment consist in positioning the upper left marker over the upper left metal or biotin marker and then adjusting the whole grid size by using the lower right metal or biotin marker Once the grid is squared properly according to array s dot micromatrix press Reanalyze button Fig 47 and 48 In order to place the upper left marker over its proper position just press on it and drag it with the finger until both positions match Once placed the first marker user can open or close the grid size by dragging the lower right marker Grid movements are limited in order to prevent potential errors when aligning y i ST TT a LEED Fig 47 Manual grid alignment screen Clinical Array reader CAR Manual Version 5 April 2011 39 Clinical Array Reader CAR User Manual GEN MICA Fig 48 Aligning the metal markers on the reanalysis menu Zoom tool placed on the upper right corner of the screen facilitates the reanalysis to user Fig 49 Fig 49 Manual grid alignment in strips using metal markers and zoom tool Once the metal markers are aligned press Reanalyse option Fig 49 and a image with the grid
24. ates In order to specify the search three extra searching parameters are available Sample ID Search for samples with same code or ID analyzed during the specified time period Assay ID Search samples processed with same procedure or assay during the specified time None Search all samples included in the indicated time period Clinical Array reader CAR Manual Version 5 April 2011 Clinical Array Reader CAR User Manual GEN MICA Select Filters Sample ID 5647898 o Assay Name 40204 None Ph PI I IL JE JPL JE Leese BHAA BeASrrs es EH EN ea ber el le ele al ll ese py aste Fig 83 Press Next to continue 11 3 BARCODE READER CHARACTERISTICS AND INSTALLATION A simple barcode reader will be advisable since installation process is done automatically by the device just by connecting it at the rear PS 2 or USB port Clinical Array reader CAR Manual Version 5 April 2011 57 Clinical Array Reader CAR User Manual GEN MICA Barcode Reader specifications recommendation Scanning Element Light Source 660 nm red visible LED UPC E UPC A EAN 8 EAN 13 JAN UCC EAN 128 ISBN ISSN Code 39 Code 93 Code 128 Interleave 2 of 5 MSI Industrial 2 of 5 Matrix 2 of 5 IATA 2 of 5 China Post Code Code 32 Codabar ABC Codabar CX Codabar Code 11 UK Plessey Telepen Decode Capability Power Conditions 20 to 80 non condensing
25. ay cid lid ENET ia kiia lt lt Back Next gt gt Fig 67 Introducing dates for the backup All selected runs are shown on an extra menu Fig 68 Both options Backup only or Backup and Delete are possible User decides whether to keep data on the CAR s reader or erase the already saved results All None Run Date l 922 4 2 2008 1 27 30 PM M 923 4 2 2008 1 35 42 PM m 925 4 3 2008 11 53 11 AM M 926 4 4 2008 1 03 48 PM M 927 4 4 2008 3 56 33 PM VH QPR AIAIPNNR 2 5R 5R PM lt Backup only Backup and Delete Main Menu lt lt Back Next gt gt Fig 68 List with selected results and the two backup options Press Next to finalize the backup process a User can change the backup path at any time on the Settings menu See FAQ Clinical Array reader CAR Manual Version 5 April 2011 AS Clinical Array Reader CAR 10 2 4 SETTINGS User Manual GEN MICA Settings menu Fig 69 allows the user to Adjust data time manually Insert both data export and backup path Set Language only English Spanish French and Greek are available Set default assay Pneumovir HPV2 HPV2 MM ENTHERPEX Date Time Export Path Backup Path Language Default Assay Clinical Array reader CAR Manual System Information 04 21 2009 15 36 05 Adjust F jE C Genomica data Backup English CLART_Pneumovir_13 CLART_H
26. ber in the previous section allows the user to skip this step because assays ID s are already set Setting Assays IDs Manually Software processes by default the CLART HPV2 Assay To read or analyse any other GENOMICA S CLART Assay select the position by pressing on it and then press Edit Assay option Edit Assay Select the proper assay from those appearing in the pull down menu as shown Fig 33 Clinical Array reader CAR Manual Version 5 April 2011 3 Clinical Array Reader CAR User Manual GEN MICA Selected column 1 Select Assay Ic LART_IIP V 2_hMlhy Barcode Reader kl Assign Assay to all samples Fig 33 Multiple assays choices Software gives also the possibility to select all tubes and edit the Assay ID in all of them at same time In case of processing all samples with same assay user should select the Assign Assay to all samples on the Edit menu Fig 33 9 5 SETTING SAMPLES IDs In the same way that in previous sections setting Samples IDs can be carried out automatically or manually In case of no Samples IDs assigment software will set automatically a aul correlative number to each sample from 1 to 12 starting with the sample placed in position 1A upper left Setting Samples IDs Automaticall ch Barcode reader is required to set Samples IDs automatically and barcodes must be set and printed before start setting the IDs Set and print barcod
27. en Sample already analysed Grid alignment agreed Wells in red Sample may be reanalyzed Grid alignment failed see Sample Reanalyze section below Wells in yellow Sample has not been analyzed yet Clinical Array reader CAR Manual Version 5 April 2011 21 Clinical Array Reader CAR User Manual GEN MICA Run Number 837 Plate ID 100002 35 Abort Fig 13 Monitoring the reading process in the CAR s screen Different colours for different analysis status Once finished the reading process user will be requested again to initialize the opening and closing procedures Figs 12 and 13 Results will be shown once the microplate has been removed from reader and tray is closed 8 6 SUMMARY REPORT AND RESULTS SUMMARY REPORT The CAR generates a final report including following information Date and time Position of the sample Assay ID Sample ID Results for each sample User can look up the final report by selecting Report View option It s possible to print out this run report in PDF format just by pressing the print option on the upper right side of the screen Fig 14 Clinical Array reader CAR Manual Version 5 April 2011 22 Clinical Array Reader CAR Genomica SAU CAR CAP System Assay Pos ID Y Assay Al 40204 Papilloma Bi 40204 Papilloma c ra 40204 Papilloma D we 40204 Papilloma E ay 40204 Papilloma F ma 4020
28. enu of the reanalyze option for ASs By operating Start Reanalyze button user accedes to the reanalysis application The correct grid alignment consist in positioning the upper left marker over the upper left metal or biotin marker and then adjusting the whole grid size by using the lower right metal or biotin marker Once the grid is squared properly according to array s dot micromatrix press Reanalyze button Fig 22 Clinical Array reader CAR Manual Version 5 April 2011 25 Clinical Array Reader CAR User Manual GEN MICA In order to place the upper left marker over its proper position just press on it and drag it with the finger until both positions match Once placed the first marker user can open or close the grid size by dragging the lower right marker Grid movements are limited in order to prevent potential errors when aligning Fig 22 Placing metal markers according array s matrix Zoom tool placed on the upper right corner of the screen facilitates the reanalysis process Scroll the screen to visualize upper and lower markers cai i Zooo Fig 23 Manual grid alignment in strips using metal markers and zoom tool Once the markers are aligned press Reanalyze option Fig 24 and an image with the grid will show the final alignment If both biotin and metal markers fits press Accepf lower right side If new adjustments are required press Retry Fig
29. es Press Set Samples IDs option and read the previously assigned barcodes normally The software associates automatically a barcode to a sample position Samples with already assigned ID will appear in black Fig 34 Clinical Array reader CAR Manual Version 5 April 2011 32 Clinical Array Reader CAR User Manual GEN MICA Select Rack Set Manually Stop Import Fig 34 Setting Samples ID automatically Once finished importing IDs press Stop Import to return to the New Analysis menu Setting Samples IDs Manually Select each position individually by pressing twice on it A new window representing a tactile key board will pop up on the screen Fig 35 Use it to set samples ID manually Selected Strip Position A1 Samples ID 98987 Fig 35 Setting Samples IDs manually using the tactile keyboard The user can check the sample position at any time since it appears at the upper left side of the screen Once finished with the samples identification process press OK to go back to the New Analysis menu Fig 29 Then press NEXT Clinical Array reader CAR Manual Version 5 April 2011 33 Clinical Array Reader CAR User Manual GEN MICA 9 6 WORKING LIST The working list allows the user to check whether the already introduced information samples references
30. in this manual to set up the Clinical Array Reader CAR 4 1 PRECAUTIONS Verify that the instrument does not present any obvious sign of damage If any damage is found please contact immediately to your local GENOMICA s representative Prepare approximately 1 m free area in the lab for the installation of the CAR Once unpacked place your CAR in a standing position on the bench space you have just prepared Relocation of your CAR is only recommended by lifting it DO NEVER PUSH YOUR CAR Make sure that the CAR is placed on a stable and perfectly horizontal bench Before connecting it to the power supply make sure that your power line voltage corresponds to the operating voltage of 110 230 V AC at 50 60 Hz Do not install the CAR in the influence area of a heat or intense light source 4 2 UNLOCK LOCK THE READER In order to avoid system damages during transport internal mobile components of the CAR are locked In order to unlock the reader remove the big screw on the rear side and on the right side Readers contain two locking systems one on the rear side and one in the right side During transportations the position of the tray holder is fixed by lock screws in order to protect the mechanical components Remove these screws before switching on the instrument for the first time and keep them for the posterior assembling of the screen No special tools are required to remove both screws Clinical Array re
31. installed screen s arm should appears as follow Clinical Array reader CAR Manual Version 5 April 2011 Clinical Array Reader CAR User Manual GEN MICA 4 4 CONNECTING SCREEN AND READER A O OLA 1a 1b 1c Clinical Array reader CAR Manual Version 5 April 2011 10 Clinical Array Reader CAR User Manual GEN MICA 1 Connecting cables 1a Connect the power supply Type Jack 3 5 1b Connect the USB p to one port at the right side 1c Connect the VGA connection to the blue connector 2 Switch on the screen by pressing the button on top 3 Switch on the CAR 5 SAFETY NOTES Please be particularly aware of electrical hazards Standard electrical safety precautions must be applied at all times Only qualified personnel are permitted to perform electrical servicing Never touch switchers or outlets with wet hands Always switch off the instrument before disconnecting the AC power cord Always unplug the instrument prior to cleaning major liquid spills and prior to servicing the electrical or internal components Do not open the housing of the instrument Do not attempt any repairs or alterations except as expressly instructed in this manual or by the technical service Avoid any movement or vibration during reading operation 6 PROCEDURAL LIMITATIONS Clinical Array Reader CAR has been designed for the detection and analysis of ASs CSs Array Strips CLART Strips or ATs Array
32. ion on the microplate sketch Fig 15 by pressing twice on it A new menu showing working list and results will appear on the screen Fig 16 On this menu following options are shown Export To export reports and results to an external server LIMS or USB Print If an external printer is connected via USB port the selected result s page or all results data pages including test image can be printed by operating the Print button See FAQ Image To visualize the image obtained by the CAR Fig 18 Raw Data To consult raw and normalized numerical values obtained in the analysis of the samples Clinical Array reader CAR Manual Version 5 April 2011 23 Clinical Array Reader CAR User Manual GEN MICA Virus Controls Type 6 Passe ype 11 Passe Type 16 Passe ype 18 Passe Passe ype 44 Passe ype 45 Passe Tna ED Maanntivun Darren Image i gt Fig 16 Results menu By selecting the Image option user accesses to the image corresponding to the analyzed sample Fig 17 and 18 4 S rus Export w mes J Type 11 Type 16 Passed Print 028 ype 31 ype 33 8 ype 40 ype 42 ype 43 Type 44 Passed Passed Type 45 Type 51 Negatve Passed Tuna ED Manatiuun Nannan z iy Q S S lt gt Om Q Q w v ot e lt lt Dm T Negative T Negative 4 is Q D ct lt D e v zzl S a a io m 2 z Z
33. l Version 5 April 2011 4 Clinical Array Reader CAR User Manual GEN MICA 3 COMPONENTS DESCRIPTION 1 Screen Tray ige Performance Resolution on the sample 3 um Camera pixel resolution 8 3 Mpixel Scan time for complete 96 well plate 2 3 minutes Dynamic Range 8 bits 255 greyscale Mechanical With O Heim Deth Additional 10 cm space must be provided in front of the instrument for microplate loading as well as an additional 10 cm space at the back for cables and ventilation Weight without tactile screen 15 Kg Replacement fan filter 80x80mm NMB Part No F80 MR Environmental l NVIronmenial Condition ERT Operating Temperature Storage Temperature 0 70 C 40 a 85 C 10 75 Clinical Array reader CAR Manual Version 5 April 2011 5 Clinical Array Reader CAR User Manual GEN MICA Electrical 5x20 mm 250V 1 6A slow 110 230 V AC a 50 60 150 W blow Hz Image storage format 8 bit BMP greyscale Results storage format CSV spreadsheet Internal PC Windows XP home edition VGA RS 232 mouse and keyboard Connections Serial100 Base T Ethernet 3x USB2 Touch screen 800x600 264 K 12V VGA USB B Mechanical mount VESA 75 Max current for 12VDC tactile screen supply 3 Amp Clinical Array reader CAR Manual Version 5 April 2011 6 Clinical Array Reader CAR User Manual GEN MICA 4 INSTALATION ay Please follow the indications described
34. le is already analyzed Grid alignment succeded Wells in red Sample may be reanalyzed Grid alignment failed see Sample Reanalyze section below Wells in yellow Sample has not been analyzed yet Clinical Array reader CAR Manual Version 5 April 2011 35 Clinical Array Reader CAR User Manual GEN MICA Run Number 1442 Plate ID 100003 O CUETO HT 40 Fig 39 Reading process 9 8 SUMMARY REPORT AND RESULTS SUMMARY REPORT The CAR generates a final report including following information Date and time Position of the sample Assay ID Sample ID Results for each sample User can look up the final report by selecting Report View option From this menu it is also possible to print it out in PDF format Fig 40 Working list GenomicaSAU Rev 14 CAR CAP System Run ID 190003 Date and time Wed Feb 25 09 54 28 2009 GEN MICA pany sasay Assy ID _ Sample reSrence 40207 CLART HPV2 MM 000000040207 Bl 40207 CLART HPV2 M C CLART HPV2 MM 000000040207 A 40207 CLART HPV 0040207 44 EADS PEE RTE i Operational Mode Process and Reading OReading Fig 40 Summary report created by the CAR at the end of the analyzing process Clinical Array reader CAR Manual Version 5 April 2011 36 Clinical Array Reader CAR User Manual GEN MICA RESULTS To visualize a specific sample s result select the sample position on the microplate sketch
35. linical Array Reader CAR User Manual GEN MICA System 0 gadi Backup Reader Menu Settings Fig 73 In the dialog box you should see both drives C and D Corresponding to the USB stick In some cases it takes a couple of minutes to the reader to recognize the pen drive In those cases go back to the principal menu and try again in a few seconds CA DA Fig 74 Browse the pen drive contents by pressing on the plus symbol You can see there the patch folder and the kits installation programs in blue Clinical Array reader CAR Manual Version 5 April 2011 52 Clinical Array Reader CAR User Manual GEN MICA E C patch HPV2_install bat Metabone_install bat Pneumovir_install bat D Fig 75 Select the kit that you want to install and press Import E C amp DA patch Metabone_install bat Pneumovir_install bat D HPV2_install bat Fig 76 Installation will be finished when a new window pops up indicating that the process has been completed successfully Please close that window by pressing Cross button on the top right square If the installation of more kits is required repeat the process from step 4 Once finished get back to the Main Manu and reinitialize the reader In case that an error occurs during the installation process please contact the technical service Do not forget to attach the appearing error messages in your assistance request
36. ls and Warning Signs Clinical Array reader CAR Manual Version 5 April 2011 GEN MICA 45 49 50 53 56 56 57 57 Clinical Array Reader CAR User Manual GEN MICA 2 INTRODUCTION The Clinical Array Reader CAR has been designed for a quick detection and analysis of ASs This detection and analysis represent the second step of an CSs assay The first step of a complete CSs assay also involves experimental procedures sample preparation DNA extraction DNA amplification and target labelling specific hybridisation and visualization of the results The detection and analysis of CSs by the CAR is always performed immediately after developing the specific precipitation staining pattern on the CSs Developing of this pattern generates a dot micromatrix microarray on the t the bottom of the CSs This micromatrix can be detected and analysed by the CAR The results obtained by the CAR are automatically shown on the touch screen and can be printed or exported to your LIMS or pen drive by its USB connections Sample preparation DNA extraction and DNA amplification labelling of targeted Standard molecules laboratory equipment Specific hybridisation labelled sample incubation against arrays Visualization conjugation and precipitation of the staining Experimental Procedures Colorimetric Detection and Analysis c O N gt Clinical Array Reader Clinical Array reader CAR Manua
37. m the Edit menu Clinical Array reader CAR Manual Version 5 April 2011 16 Clinical Array Reader CAR User Manual GEN MICA Select Rack 96 well plate 390 0 0 0 0 0 0 0 0 0 0 gt Ea Ch Ch Ch Ch Gh Ch Cah Gh oh tt TB 4 HE EE BEE mnu E E gga EJ E9 E9 E A GA e e E Set Assay IDs Set Sample IDs Oo Net Fig 7a Selecting Edit option on the New Analysis menu Selected column 1 Select Assay cl ART_HP Y MiV v Barcode Reader W Assign Assay to all samples Cancel OK Aa AP A lA EE Pe ll lei CII IC I SIPC IEOIC CICS SPACE DAN Cepe Paste Fig 7b Manual setting of Assays IDs A pull down menu shows the multiple choices to choose among Press OK when finish to return to previous menu and follow up with the visualization process Once assigned the assays IDs strips will turn to the different colour combination specific for each type of analysis Fig 7C Clinical Array reader CAR Manual Version 5 April 2011 17 Clinical Array Reader CAR User Manual GEN MICA Select Rack 96 Well Plate 00080 Set Plate ID 0 0 0 0 0 0 T y L d pi Da aii x n 2 Se 5 gt di ki ae 4 a a a ULLI T a fe ves a 6 a E Set Assay IDs Set Sample IDs Fig 7c Diferent colours combinations corresponding to different assays 8 3 SETTING SAMPLES IDs In the same way that i
38. n previous sections setting samples IDs can be carried out automatically or manually In case of no Samples IDs assigment software will set automatically a ah correlative number to each sample from 1 to 96 starting with the sample placed in position 1A upper left Setting Samples IDs Automaticall ay Barcode reader is required to set Samples IDs automatically and barcodes must be set and printed before start setting the IDs Set and print barcodes before starting Press Set Samples IDs option and read the previously assigned barcodes normally The software associates automatically a barcode to a sample position Samples with already assigned ID will appear in black Fig 8 Clinical Array reader CAR Manual Version 5 April 2011 18 Clinical Array Reader CAR User Manual GEN MICA BEEseI9999090 et Assay IDs Stop Import Cancel Next Fig 8 Setting Samples ID automatically Once finished importing IDs press Stop Import to return to the New Analysis menu Setting Samples IDs Manually Select each position individually by pressing twice on it A new window representing a tactile key board will pop up on the screen Fig 9 Use it to set samples ID manually Firstly erase the number assigned automatically by the reader using the Backspace key then insert the ID desired Selected Strip Position A1 Samples ID 98987 Cancel OK Fm GH Gm Re E0OCAA CA z u Cqp 4
39. per left position Fig 25 Placing tubes in the adaptor Select New Analysis option at the main menu of Genomica s application Fig 26 Service GEN MICA Archive Shut Down ind Fig 26 Selecting New Analysis at the main menu of GENOMICA s application Clinical Array reader CAR Manual Version 5 April 2011 28 Clinical Array Reader CAR User Manual GEN MICA 9 2 CHANGING RACK TYPE The software establishes the analysis of a whole 96 well plate as default composed by up to 12 strips In case of analyzing ATs tubes user should change the rack type To change the rack type just select the pull down menu Select Rack on the top of the screen and choose Tube adaptor Fig 27 instead of 96 well plate Set Plate ID a Tm SB os Set Assay IDs Set Sample IDs Fig 27 Select rack option at the upper left side of screen A new window will pop up showing the tube adaptor sketch Fig 28 Select Rack Tube Adapter x Set Plate ID Use Tube Edit Assay Set Assay IDs Set Sample IDs Fig 28 Main menu when reading ATs Clinical Array reader CAR Manual Version 5 April 2011 29 Clinical Array Reader CAR User Manual GEN MICA 9 3 SETTING SAMPLE S NUMBER The number of samples to be analysed can be introduced manually or automatically by using a barcode reader Automatic setting
40. screen Fig 15 by pressing twice on it Anew menu showing working list and results will appear on the screen Fig 41 On this menu following options are shown Export To export reports and results to an external server LIMS or USB Print If an external printer is connected via USB port the selected result s page or all results data pages including test image can be printed by operating the Print button See FAQ Image To visualize the image obtained by the CAR Fig 18 This image can be exported or printed individually with print option Raw Data To consult raw and normalized numerical values obtained in the analysis of the samples Virus ooo vs Ret donot Passed Passed Passed Tunan ED Manntivun Darren Ee Export Image ggg Fig 41 Results menu By selecting the Image option user accesses to the image corresponding to the analyzed sample Fig 42 and 43 Clinical Array reader CAR Manual Version 5 April 2011 37 Clinical Array Reader CAR Virus Controls Type 6 Negative Passed Type 11 Negative Type 16 Negative ype 18 Negative Type 26 Negative Type 31 Negative Type 33 Negative Type 35 Negative Type 39 POSITIVE Type 40 Negative Type 42 Negative Type 43 Negative Type 44 Negative Type 51 Negative Passed Tuna FD Maantiun Naeena User Manual Export ry Print C Image Fig 42 Selecting Image option GEN MICA
41. urces 10 2 2 SYSTEM System menu provides the user with information regarding software hardware and CAR s serial number Fig 59 Fig 59 Selecting System option Clinical Array reader CAR Manual Version 5 April 2011 44 Clinical Array Reader CAR User Manual GEN MICA System Information Software version Software Version Hardware version Hardware Version Serial Number Serial Number lt lt Back Fig 60 Information included in System menu Press Back to return to the Service menu 10 2 3 BACKUP BACKUP PATH Reader s storage capacity is limited to 40 Gb therefore Backup option represents a useful tool to save both data results reports and CAR s settings Moreover CAR should not be considered as a database where results can be stored In order to avoid undesirable data lost backup should be perform regularly GENOMICA will not be responsible for the user s data when the technical servicing is provided Before saving results with the backup option user must set the Backup Path SETTING BACKUP PATH Backup path sets up the target destination for the backup A remote folder or any other network drive can be specified To set the backup path go to Settings option Fig 61 Clinical Array reader CAR Manual Version 5 April 2011 45 Clinical Array Reader CAR User Manual GEnemicA Import System Backup Reader Menu Fig 62
42. will show the final alignment If both dots and metal markers are well aligned then press Accep lower right side If new adjustments are required press Retry Fig 50 Clinical Array reader CAR Manual Version 5 April 2011 40 Clinical Array Reader CAR User Manual GEN MICA Fig 50 Grid alignment checking chy Manual grid alignment implies user s intervention in the results If a manual grid alignment is required an informative message will be shown in the run report The image has been reanalysed by user Fig 51 Informative message indicating the manual reanalysis 10 1 ARCHIVE By operating the Archive option Fig 52 user accedes to a result s search engine Search must be enclosed between two dates established by the user a starting date and an ending date Fig 53 Set the searching dates required and press Next Select Start End Date New Analysis Service Start Date 2007 09 15 End Date GEN MICA Year Month Day j Shut Down lt lt Back Next gt gt Fig 52 Archive option on the main menu Fig 53 Menu to set the searching period time Clinical Array reader CAR Manual Version 5 April 2011 4 Clinical Array Reader CAR User Manual GEN MICA Search engine will find out all results contained between the two indicated dates In order to specify the search three extra searching parameters are available Fig 54 in the following
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