Autoperfuser™ OS2100
Contents
1. Green C J Animal Anaesthesia Laboratory Animal Handbooks 8 Laboratory Animals Ltd London UK 1979 123 124 7 Programs Autoperfuser OS2100 is fitted with six programs DeAir Insertion P1 P2 P3 and Cleaning P1 P3 programs can be edited run to completion extended fast forwarded paused or terminated Manual for Autoperfuser OS2100 www osenses com 7 1 DeAir program Running the DeAir program is recommended before execution of any of P1 P2 or P3 programs Figure 7 DeAiring purges the tubing system with liquids to eliminate the air bubbles which may block the small blood vessels hence preventing a thorough perfusion DeAiring is performed at 100 mmHg for 50 ml of each bottle connected starting from bottle C and finishing with Bottle A DeAir program requires no editing by the end user Bottles C and or B can be skipped by pressing the gt gt Figure 7 DeAir step DeAiring is performed to purge the tubing system with liquid Click on the lt lt to skip bottle C and or 7 2 P1 and P2 programs Using programs P1 or P2 the operator is able to edit EDIT in Figure 6 and save the changes SAVE in Figure 8 The editable parameters are pressure mmHg and volume ml P1 and P2 programs are recommended when a flow rate of at least 60 ml min is expected when the animal is connected Adult rat guinea pig rabbit can be perfused using P1 or P2 programs Manual for Autoperfuser OS2100 www os2. products and product data without notice Osenses makes no warranty representation or guarantee regarding the suitability of its products for any particular purpose nor does Osenses assume any liability arising out of the use or application of any Osenses products Osenses specifically disclaims any and all liability including without limitation incidental or consequential damages Osenses and its suppliers hold no responsibility for damage expenses lost profits or any other claims arising from the use of these products Any customer purchasing or using Osenses product s shall indemnify and hold Osenses and its officers employees related companies affiliates and distributors harmless against all claims costs damages expenses and reasonable legal fees arising out of directly or indirectly any claim of loss personal injury or death associated with such unintended or unauthorized use even if such claim alleges that Osenses was negligent regarding the design or manufacture of the relevant product s Autoperfuser is designed for research use ONLY Manual for Autoperfuser OS2100 www osenses com Manual for Autoperfuser OS2100 www osenses com Manual for Autoperfuser OS2100 www osenses com
3. Keep preferably the animal and the Autoperfuser OS2100 at the same level e Connect the free ends of the three clear tubing provided to the liquid inlets A B and C Push the tubing firmly in Similarly connect the free ends of the three green tubing provided to the Air outlets A B and C Figure 1 The ends with the quick connectors should be attached to the corresponding bottles A B or C filled with the desired liquids CAUSION IF YOU NEED TO PULL OUT THE TUBING YOU HAVE TO PUSH THE WHITE GREEN OR BLUE PLASTIC SKIRTING IN WHILE GENTLY PULLING THE TUBING OUT THE TUBING HOUSING MIGHT PERMANENTLY GET DAMAGED IF THE TUBING IS PULLED OUT FORCEFULLY WITHOUT PUSHING THE SKIRTING IN Figure 1 Left side panel of the Autoperfuser OS2100 Manual for Autoperfuser OS2100 www osenses com e Connect the clear tubing to the liquid outlet Figure 2 by push it firmly in Using the two way stopcock provided connect one extension tubing to the end of the tubing Now attached an appropriate needle to be connected to the animal Figure 4 Figure 2 Right side panel of Autoperfuser OS2100 e Connect the power cord to the power socket and plug into the wall power socket Figure 3 Figure 3 Backside of the Autoperfuser OS2100 Manual for Autoperfuser OS2100 www osenses com e A fully assembled Autoperfuser OS2100 will look as shown In Figure 4 Figure 4 Fully assembled Autoperfuser OS2100 Manual for A
4. Last modified 28 January 2014 7 w Osenses Reagents For Every Sense A fully automated animal perfusion system For research use ONLY USER MANUAL Manual for Autoperfuser OS2100 www osenses com 2 Table of Contents CR 4 7 4 Ss 0 5 4 Interaction with Autoperfuser pp 8 9 FING HONG 26 its hh nat ste eisuste ele yemedaobacbaadan ala lanae remsce en saeasaeee ied 9 6 Typical perfusion pressure pp 9 Ts 9 fala 10 7 2 10 RZ Programs 2 niesntaiien ie eee ee 10 kos PoprograM ssc ease A A tat eee eee ea 12 FA Insertion program pp 13 Los RUNING a DrOONAIN iene 13 7 02 Pausing a DIOGO erat eis ate eect 15 7 7 Terminating a program pp 16 7 6 Cleaning prograrm ea 17 8 Pressure volume and time range pp 17 9 Bottles and connectors pp 18 10 On screen messages pp 18 TOT LA 18 10 2 PRESSURE cauva aa a eee eae 19 19 2 DO NOT OREN oseere 19 A Va Trouble 00 00 ten 20 2 21 1 21 Disclaimer dies sat atten a A doth Beceuicidae 21 Manual for Autoperfuser OS2100 www osenses com 1 Packing list Components provided 1 Autoperfuser OS2100 with the OS2100 software preinstalled 2 3x 2L HDP bottles fitted with quick connectors 3 2x Extension tubing 4 2x Two way stopcocks 5 5x blunt end needle 18G N Safety rules e When using Autoperfuser O82100 basic safety precautions should always be followed e Read all instruct
5. been extended 11 2 3 P1 was run to the completion 11 3 3 In Figure 11 1 3 the 300 is the target volume ml and 150 is the passed through volume ml Manual for Autoperfuser OS2100 www osenses com Figure 12 shows the P3 program in operation Figure 12 P1 is in progress and can be paused by pressing I The 5 is the target time minute and 1 is the elapsed time minute 7 6 Pausing a program You can pause P1 P3 programs at any time Press II to pause The pause sign then will be changed to lt Figure 13 While in pause the pressure will gradually drop but the flow will stop immediately To resume the program press gt the pressure will then increase to the original pressure and flow continues Figure 13 The actual P1 program is in progress but has been paused gt shows that the program is in pause Manual for Autoperfuser OS2100 www osenses com T T Terminating a program A program can be terminated by pressing the STOP button Figure 13 When P1 P3 programs are stopped a warning message DO NOT OPEN will appear briefly and the STOP button will change to HOME button Figure 14 DO NOT OPEN warning means that the bottle caps should not be opened due to the high pressure The message will disappear when the pressure is dropped below 50 mmHg Press HOME button to return to the main menu Figure 6 Figure 14 shows P1 program has
6. been terminated and the DO NOT OPEN warning appears Manual for Autoperfuser OS2100 www osenses com 7 8 Cleaning program After a daily use Autoperfuser OS2100 must be cleaned using the CLEAN program see Figure 6 Fill all three bottles with 500 ml deionised water DO NOT USE MQ WATER Make sure that the outlet is in the sink Now press the CLEAN button The cleaning program will use 200 ml of each bottle starting with Bottle C and finishing with Bottle A The cleaning is performed with a preset pressure of 100 mmHg Figure 15 If the Autoperfuser OS2100 is used infrequently after the first wash fill the bottles with 20 ethanol and CLEAN once more Mild detergent can also be used for the cleaning but always include a rinse with water followed by 20 ethanol wash Figure 15 CLEAN program in progress Cleaning can be stopped or fast forwarded 8 Pressure volume and time range Autoperfuser OS2100 can be set at a minimum pressure of 60 mmHg and a maximum pressure of 299 mmHg The volume range is 0 to 9999 ml In P3 the minimum running time is 0 and the maximum is 9999 minutes Manual for Autoperfuser OS2100 www osenses com 9 Bottles and connectors Autoperfuser OS2100 is provided with 3x 2 L of graduated HDP bottles with the lids fitted with quick connectors All the connectors are equipped with check valves ensuring no leakage if any of tubing is disconnected Bottle A is typically u
7. enses com BOTTLE A 1 PRESSURE mmHg VOLUME ml SAVE HOME Figure 8 1 3 BOTTLE 8 1 PRESSURE mmHg VOLUME ml SAVE HOME Figure 8 2 3 BOTTLE 6 1 PRESSURE mmHg VOLUME ml SAVE HOME Figure 8 3 3 Figure 8 Using program P1 the operator can modify the pressure and volume and save the changes P1 and P2 programs are recommended when a flow rate of at least 60 ml min or more is expected when the animal is connected Manual for Autoperfuser OS2100 www osenses com lid P3 program Using P3 program the operator is able to edit EDIT in Figure 6 and save the changes SAVE in Figure 9 The editable parameters are pressure mmHg and time minute P3 BOTTLE A PRESSURE mmHg me nn 7 HOME Figure 9 1 3 P 3 BOTTLE B PRESSURE mmHg mmn HOME SAVE SAVE Figure 9 2 3 P 3 BOTTLE 6 PRESSURE mmHg ne min HOME SAVE Figure 9 3 3 Figure 9 Using program P3 the operator can modify the pressure and time and save the changes Manual for Autoperfuser OS2100 www osenses com P3 program is recommended when a flow rate of below 60 ml min is expected when the animal is connected Mouse can be perfused using P3 program 7 4 Insertion progra
8. ions and information in this manual before using the instrument e To protect against fire electric shock and personal injury do not immerse cord plugs or the appliance in water or other liquid e Unplug from outlet when not in use e Do not operate the instrument with a damaged cord or plug or after the appliance has been damaged in any way Contact the nearest authorized service centre e Do not use accessory attachments not recommended by Osenses Do not use outdoors e Do not let cord hang over edge of table or touch hot surfaces e Always turn the power OFF then plug the cord into the wall outlet To disconnect turn the switch to OFF then remove plug from the wall outlet When in operation e Do not open bottles or disconnect air liquid hoses Any servicing other than cleaning and user maintenance should be performed by an authorized service centre Do not disassemble the appliance There are no user serviceable parts inside Repair should be done by authorized service personnel only Manual for Autoperfuser OS2100 www osenses com 3 Getting started e Open the Autoperfuser OS2100 package and make sure that all the items according to the packaging list are included e there are any parts missing damaged or incorrect please contact your local distributor e Place the instrument on a dry and flat table capable of carrying the weight of the instrument Autoperfuser OS2100 weighs 10 Kg
9. m This step is included to have Autoperfuser OS2100 running under a low pressure delivering dropwise liquid while the animal is being prepared The pressure hence the flow rate can be adjusted as required Figure 10 Figure 10 depicts INSERTION program The Flow may be altered by adjusting the pressure using lt lt or gt gt 7 5 Running a program To run a program simply press the desired program in the LCD touchscreen While P1 P3 is being used lt lt can be pressed to re run a current step without interruption Similarly if desired lt lt can be pressed to skip the current step When lt lt is pressed the LCD touchscreen will turn to green to indicate that the extension step is chosen Forward step lt lt can also be chosen when running DeAir program A program can also be stopped by pressing STOP button To switch from Insertion program to the actual perfusion P1 P3 programs the INSERTION program has to be stopped first to return to the HOME page and then press the desired program Manual for Autoperfuser OS2100 www osenses com As examples three screenshots of the LCD panel are shown in Figure 11 1 3 Pi IN PROGRESS 150 ml 300 PRESSURE mm Hg 110 STOP Figure 11 1 3 Figure 11 2 3 P 1 COMPLETED BOTTLE A BOTTLE B BOTTLE C 300 300 300 300 300 300 DO NOT OPEN HOME Figure 11 3 3 Figure 11 P1 in progress 11 1 3 running Bottle A has
10. nute or so Figure 14 16 17 Manual for Autoperfuser OS2100 www osenses com 11 Trouble shooting Apparatus does No power Make sure that the not start apparatus is plugged in and there is a power No flow Bottles are empty Fill the bottle with blocked tubing the desired liquid and clean the tubing ml min Two way stopcock is Set the stopcock in closed the open position Low pressure The caps are not properly Make sure that caps tightened damaged are tight and the tubing tubing is not damaged Manual for Autoperfuser OS2100 www osenses com 12 Warranty Osenses warrants the Autoperfuser 0052100 to be free from defects in material and workmanship for a period of one year from the date of original purchase This warranty covers parts and labour We may repair or replace defective apparatus at our option Osenses disclaims all responsibility for consequential damages of incidental losses caused by the use of Autoperfuser OS2100 except where such disclaimer is not allowed by law There is no warranty on consumables supplied with the instrument The warranty does not cover damage from abuse neglect or use for purposes other than those intended and described in the manual 13 Help you have any questions or suggestions feel free to contact us by email technical osenses com We would be delighted to hear from you 14 Disclaimer Osenses Pty Ltd reserves the right to make changes to both its
11. sed for the first washing step Bottle B is used for the actual fixative and Bottle C is optional Any bottle not in use can be disconnected If connected and still not in use then the bottle either should be filled with liquid or skipped using gt gt alternatively it should be programmed at 0 ml in 1 and P2 programs or 0 minute in P3 program 10 Onscreen messages Three messages might appear on the LCD screen 1 NO FLOW 2 PRESSURE 3 DO NOT OPEN 10 1 NO FLOW NO FLOW warning message appears if the flow rate is below 60 ml min Figure 16 Make sure that you have the bottles filled with the liquid and the two way stopcock is open If the problem persists while you are using P1 or P2 program then switch to P3 program instead ERROR 1 NO FLOW BOTTLE A BOTTLE B BOTTLE C 300 300 150 300 300 300 DO NOT OPEN HOME Figure 16 Warning message NO FLOW indicates a flow rate of below 60 ml min Manual for Autoperfuser OS2100 www osenses com 10 2 PRESSURE PRESSURE warning message indicates that the actual pressure is not identical to the programmed one Figure 17 Make sure that the bottles are tightly capped and the two way stopcock is in the open position Figure 17 Warning message PRESSURE appears if the programmed pressure is not reached 10 3 DO NOT OPEN DO NOT OPEN warning message appears if the pressure inside the bottles are above 50 mmHg This message should disappear within a mi
12. utoperfuser OS2100 www osenses com e Switch the power on the LCD touchscreen panel will be lit shortly thereafter Figure 5 ls Osenses Figure 5 Front side of the Autoperfuser OS2100 4 Interaction with Autoperfuser The operator interacts with the Autoperfuser OS2100 via a water resistant LCD touchscreen panel Figure 6 Figure 6 Homepage of the Autoperfuser OS2100 Manual for Autoperfuser OS2100 www osenses com 5 Principle Autoperfuser 0052100 operates under a pre defined pulsating pressure delivering liquid from the Bottle A C in a pulsating manner to the tissues It mimics the pulsating blood flow to achieve an outstanding tissue perfusion of ultimate quality Note that the flow rate will be adjusted naturally according to the cardiovascular resistance of the animal being perfused The perfusion is reproducible and consistent The degree of perfusion may be manipulated by adjusting the pressure volume or duration of the perfusion 6 Typical perfusion pressure Table 1 depicts the blood pressure in commonly used laboratory animals A pressure similar to the natural blood pressure of the animal is typically used for the perfusion Species Blood pressure mmHg Systolic Diastolic a MOUSE l S B Rat OL J0 2 Hamster mnt DOL TNO E Rabbit 0 2 Cat 00 A DOG le OB cn y Baboon 0 k Rhesus OO VET Pig 170 108 Table 1 Blood pressure in commonly used laboratory animals
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