Controlling the Agilent 1260 Infinity/1290 Infinity II Multisampler
Contents
1. ee Controlling the Agilent 70 1260 Infinity 1290 Infinity II Multisampler G7167A B in Waters Empower 3 environment using the Agilent Instrument Control Framework Technical Note Contents Technical Guide for the configuration and use of the Agilent 1290 Infinity II Multisampler G7167B and the Agilent 1260 Infinity Multisampler G7167A with Waters Empower 3 using the Agilent Instrument Control Framework ICF Introduction 2 Prerequisites 2 Defining and Assigning Plates in Waters Empower 3 Multisampler Method Setup 8 Start an Analysis in Empower 3 11 Appendices 13 A Configuring an Agilent LC in Empower 13 B Importing Agilent Well Plate Definitions 19 C Manual Set Up of Well Plates and Example Configuration Layouts for Standard Agilent Well Plates 23 D Optional Multisampler Method Setup Parameters 26 fhe Agilent Technologies Introduction Introduction The Agilent Instrument Control Framework ICF is a software component that manages the communication between the chromatography data system CDS and the instrument driver This component makes it easier and faster for CDS software vendors to implement control of Agilent LC CE and GC instruments in their data systems or workstations Based on new standard instrument drivers from Agilent ICF eliminates much of the delay and effort of using low level instrument control codes and the need of software developers to write their own native drive2. Projects Nodes B Systems Libraries Sy eCord Users User Groups User Types amp Plate Types Ge System Audit Trail Offline System Audit Trail 20 E ere DCO Fe swat oa swat wrt nk 96 well 300 uL 11 Agilent54vialPlate New ANSI 24Tube1 SmLHolder Delete ANSI 24Vial4mLHolder Export to Text ANSI 384wel1 OOuL Import from Text ANSI 384well250uL Properties ANSI 48TubeO 65mLHalder ANSI 48 Vial2mLHolder ANSI S6wellt mL ANSI 96 welll mLGlassinset 20 ANSI 96yvell2mL Print Table 21 ANSI 96wvell350uL 22 ANSI 96well700uLGlassins Copy Hide Column Show All Colurnns Table Properties Column Properties Appendices 3 The Import Plate Type From Text File dialog appears Click the Browse button and navigate to the Agilent Plates for Import folder as described in the next step System Administrator Configuration Manager File Edit View Records Tools Help Aaa of l x slee Filter By Default v Edit View _ Update Max Rows 1000 14 A gt gt Empower 3 Configuration bE l Plate Type Name S cea 24 uCfuge tube 1 5L E5 Nodes 24 vial open access 2mL og Systems E Libraries 384 well 80uL Sy eCord Ea 48 uCfuge tube 0 5mL amp Users E 48 vial holder 2mL User Groups E 96 well tall 1mL User Types amp Plate Types 96 well tall 2mL f System Audit Trail Offline System Audit T
3. B Run Ony v Continue on Faut x Sample Name Function Inject Samples Method Set l Plate Well 1 Develop Methods Injection Volume 10 0 Run Time 10 00 Options 2 g 4T gt Single Samples Sample Sets Runnin Ld Instrument Method Sample Set Time Remaining 0 00 00 00 Total Samples Time Remaining 0 00 00 00 New Sample Set Time 0 00 00 00 Sample Set 0 000 0 002 leaf hen 0 008 0 010 ers gt Multisampler EA Binary Pump ml Column Comp 00 o de WOO lde M OO QONotReady OO 5 00 yL 23 26 100 00 0 00 7 0 000 mL min gt o o icone Instrument Status Module Options Diagnostics Log For Help press F1 System Idle ft ky E The Agilent Instrument Status screen and ICF software under Empower is shown above with the Multisampler configured at the bottom of the screen The instrument method and the method set can be created in Empower by accessing the Empower Run Samples screen The Instrument method is set up and saved through the Edit Instrument Method dialog The Multisampler dialog screens for setting appropriate method parameters are shown below Edit Instrument Method on page 9 Additional optional Multisampler method setup steps are provided in Appendix D Optional Multisampler Method Setup Parameters on page 26 Multisampler Method Setup Edit Instrument Method 1 Go to Edit and click Instrument Meth
4. Agilent54 Agilent54 Agilent54 eal FE ak VE HE Ue Cancel Help Plate Sequencing Mode a III lf f wA 2 ff 7 tz e MY g ny ho yo f m fa 2 E is fe J kw bw 4 o i FX LA ro ww a oa 74 5 LS a pr D QJ A ry N ra a f 2 2 J m f f kS m m FTN GJ m n nn raii fns tJ n Inject Standards A aS CB T ES FS f aah f m fe n m a VO Ea Pema f nm f nm res nh S nm S nm Fi n Y Fi m Y fa y Fi oO k j Y Fi gt N w A 1o lio J o 1o lio f Sa f i fs N f M f f gt N 3 w if w if re if w S w i c Fa in y Fa mY far Fi F Fi fe 3 Fi A 3 8 3 8 5 5 Insert page 6 otherwise a mismatch will result when starting the analysis Mew Plate Type The selected plates defined in the table above must be the same type and position as defined in the Tray and Plate Configuration dialog of the Multisampler graphical user interface refer to step 8 on Plate Rows and Columns Referencing 00000000 00000000 00000000 90000000 900000000 900000000 900000000 00000000 90000000 89966066 9086880800608 00000000 Row and Column Dimensions mm Number Rows Columns Row and Column Offsets mm f None Odd Even Row Offset f None C Odd f Even Column Offset Spacing Offset mm o an
5. 25 Appendices D Optional Multisampler Method Setup Parameters D Optional Multisampler Method Setup Parameters The information provided below are additional optional steps in setting up a Multisampler method 1 This set of parameters can be used to reduce the amount of potential carryover in the system Click Injection Path Cleaning and enter the corresponding values in the fields provided gi e Time s SeatBackFlush Needle Wash Comment a 26 Appendices D Optional Multisampler Method Setup Parameters 2 Right mouse click on Multisampler Instrument Status and click Control Select the parameters as shown in the example and click OK Friday March 06 2015 11 00 00 4M Cs 3 Right mouse click on Multisampler Instrument Status and click Auto clean 4 Select the Duration and click the Start button 27 Appendices 5 Return to the Multisampler Instrument Status click on Prime enter the Duration period and then click Start Rod Prime Settings and Start Prime Settings Duration 3 s Note that the Multisampler primes to wash port using first solvent 51 Start Cancel The use of the reference vial array is currently not supported It is not possible to enter the reference vial position 1 5 for the sample set For more information on the available functions please consult the Agilent 1200 Infinity Series Multisamplers User Manual G7167 9015
6. 51 Function lInject Samples Y Method Set nfinity I 7 Flate well p 41 Develop Methods Injection Volume 10 0 Run Time 10 ng Options 4 EA Single 4 Sample Sets Running f 1 Ensure you are on the Single tab as outlined above and enter appropriate values including Sample Name Function Plate Well Injection Volume and Run Time 2 When entering the info for the Plate Well select the appropriate position of the sample or vial For example 4 A 1 refers to a vial in sample position Al being used in Plate 4 P4 In Empower 3 4 A 1 corresponds to P4 A 1 in the Multisampler user interface For more information on plate configurations refer to the Appendix C Manual Set Up of Well Plates and Example Configuration Layouts for Standard Agilent Well Plates on page 23 and the Agilent 1200 Infinity Series Multisamplers User Manual 3 Click the Inject Icon outlined above to start the analysis 11 Start an Analysis in Empower 3 Create a Series of Injections To create a series of injections follow the steps below for creating a Method Set This can be performed from the main Empower Run Samples screen Infinity I in Infinity I as Systern Administrator Run Samples File Edit View Inject Actions Customize Help elelem d gt Single Samples Sarnple Sets 4 Running f 1 Select File New Sample Set Method Using Sample Set Wizard from the top level me
7. to the manual of the module hosting the communication 1 Access the Configuration Manager as System Administrator as shown below aT System Administrator Configuration Manager File Edit iew Records Tools Help Aaja y l x ee H Filter By Defaut ba Edit View Update Max Rows fioo l4 Ki gt bl SB Empower 3 Configuration I nose vane reae ine Oar nose comment cet Projects Ic epStr2 b1 Empower System BEE E a a gt systems Mode Tc ep3fre bl Properties Hg Libraries He eCord General Instruments Serial Ports Configure DHCP Access ante E Users _ pi User Groups The Waters DHCP Server is designed to work automatically without user uM User Types Intervention but in some cases you will need to change or specity DHCP settings for the instrument network or third party instruments in your DP Plate Types laboratory lh System Audit Trail Mf Offline System Audit Trail Click the button below to configure third party Ethernet instruments on network C this node or to use a different P address range for your instrument E E E _ OF Cancel Help E 2 Go to Properties of the Empower Nodes 3 Click Configure DHCP button on the Configure DHCP tab 13 Appendices 4 Check the IP Address and the MAC Address of the instrument If the parameters are correct click OK To add a new instrument Click Add amp System Administrator Configu
8. 0 Edition 10 2015 Agilent Technologies Inc Printed in Germany Hewlett Packard Strasse 8 76337 Waldbronn Germany
9. 0002 134 40 26 205 A 02 11 SI 517 05 Access Point 15 Appendices A Configuring an Agilent LC in Empower The next series of steps enables the connected system to be configured and visible in Empower 1 Click File New and click Chromatographic System to make the new LC system accessible to Empower A System Administrator Configuration Manager Edit Help View Records Tools Mew Opern Ctrl 0 Project Mode Clone Chromatographic System Properties eer Backup Project User Group Restore Projects User Type Plate Type Backup Database Import Libraries Spectra Export Libraries Export Spectra Restore Pre 3 0 Library Exit 2 Select Create New System to initiate the wizard and click Next gt System Administrator Configuration Manager File Edit View Records Tools Help ajaja of 2 x see Fiter By Defaut v _EdtView _Update MaxRows 1000 tal 4 gt dll Empower 3 Configuration Yai Node Name Node Type Node Comments oy 1 ic epatr2t Empower System SIA 1 ic ep3tr2 b1 Empower l a Systems E New Chromatographic System Wizard Type Entry ne Libraries om eCord i Users Choose to define a new chromatographic system a User Groups or to connect to a system which already exists GP User Types m System Type Create New System Connect to Existing System Cancel Help J For Help press F1 16 A
10. al Set Up of Well Plates and Example Configuration Layouts for Standard Agilent Well Plates If the well vial plate of interest is neither present in Empower nor part of the available well vial plate definitions for import the well vial plate need to be manually defined within Empower As all dimensions are required to successfully define the well plates you find the parameters for the some of the Agilent well plates below If you know the dimensions proceed to the next step manual set up Rows and Columns i Ref gt Plate Rows and Columns Referencing Plate Referencing Plate Rows and Columns Referencing A Row and Column Dimensions mm Origin Name jagilent 54 vial plate Number Spacing Origin TopLeft Top Right I 3 00 C BottomLeft Bottom Right Rows 6 Format xy Columns q 13 00 Scheme Plate Dimensions mm Referencing XY C Sequential i X cl Row and Column Offsets mm peated C ABC 123 127 60 85 60 36 00 i a T None Vertical s ABC 2an Row Offset C Odd Well Dimensions mm C Even p Horizontal First Priority None x Y Top Left Well Location i 3 2 Column Offset Odd Terminology Diameter Depth C Even Plate Plate Well Size 9 20 29 00 Well via Cancel Help Cancel Help Cancel Help The 3 screens shown above refer to Agilent s 54 vial plates Plate Rows and Columns Referencing Plate Rows and Columns Referencing Plate Rows and Columns Re
11. e ansissnenm onsa ANSI 24Tube1 SmLHolder Ea E Ea a i 1 1 2 3 4 5 6 T 5 oO 19 Appendices 1 Right click on an empty row of the Plate Type Name table to select the list of plate menu functions ao system AAdministratar Configuration Manager File Edit wiew Records Tools Alala oF x Poll E Filter By Defaut _Edtview Update MaxRows i000 E Empower 3 Configuration fh Projects H eCord oe User Groups AE User Types me Plate Types a System Audit Trail E emee o ecr See e erna T 96 well gali 2mL 96 well talh 350uL 96 well wFO0uL insert _ 0 96 well 300 uL 1 Agilent54vialFlate bo ANSI 24Tube1 SmbLHolder _ 3 ANSI 24vial4mLHolder _ 4 ANSLS84yvell OOuUL S ANSI 384yell250uL 6 ANSI 48Tubed 65mLHalder J ANSI 46 ial2mLHolder co ANSI S6yyell mL _ 9 SMSI S6y yell mLGlassinset 20 SNSI S6yyell2mL 21 ANSI S6yyell350uL 2 ANS 36well jul Gassin 2 Select Import from Text from the menu list Mew Delete Export to Text Import from Text Properties Copy Hide Column Show All Columns Frint Table Table Properties Column Properties System Administrator Configuration Manager File Edit View Records Tools Help Aaa x lee E Filter By Default ne Edit View _ Update Max Rows 1000 14 4 gt gt 1 amp Empower 3 Configuration
12. er Network Users Users etn pene Allow Access to Group s a LP User Groups eee Th Administrator Base E User Types Co O Guests o Plate Types wner and Group s fh System Audit Trail Owner Group and World os Offline System Audit Trail Password Protect System Access l Password Required Password Confirm Password J 17 Appendices A Configuring an Agilent LC in Empower 5 Enter the System Name and System Comment optional then click Finish System Administrator Configuration Manager File Edit View Records Tools Help aala l x Edit View Max Rows 1000 Idi 4 gt gt i E New Chromatographic System Wizard Mame Selection Update See Empower 3 Configuration By Projects E System Name Node Name Ic ep3fr2 b1 eCord MV Online System Comment a User Groups GP User Types ABD Plate Types ea Pfr Oral Pc S Te ptt TT tT tT tT EE la After the configuration procedure the LC system should appear in the list of systems as shown in the example below m System Administrator Configuration Manager File Edit Wiew Records Tools Help slaja of 1 ele T ia Filter By Default Edit views Update Mas Rows 1000 J4 SB Empower 3Conguaton B Sraunnene Nene orure oreen came ante t intitye ee 7 ya Ic ep3tr2 b1 Mo B a tc enatr2bt No a FSystems my second instrument lc eps
13. ferencing Plate Type Row and Column Dimensions mm Origin Name Agilent 96 well plate Number Spacing Origin Top Left C Top Right C Bottom Left Bottom Right 8 00 g Format xy l nowi L Columns 12 Scheme Plate Dimensions mm Referencing XY C Sequential g Height Ff aril Caan Cl fa ow and Column Offsets mm gt 127 70 85 60 14 30 Horizontal ABC f mas None Vertical e AREA Eam Row Offset C Odd Well Dimensions mm Even B x Y oN Horizontal First Priority Top Left 50 B5 None Well Location Column Offset Odd c Terminology Even Diameter Depth Plate Plate Well Size 8 50 1 4 Well weli Cancel Help Cancel Help Cancel Help The 3 screens shown above refer to Agilent s 96 well plates 23 Appendices C Manual Set Up of Well Plates and Example Configuration Layouts for Standard Agilent Well Plates Uifset as Uifset mm The 3 screens shown above refer to Agilent 384 well plates 24 Appendices C Manual Set Up of Well Plates and Example Configuration Layouts for Standard Agilent Well Plates Manual Set Up of Well Vial Plates 1 To manually define plates go to the main Edit menu then click Plates 2 In the upcoming dialog select Create New Plate Type l Define Plates For Sarnple Set Method Create New Plate Type Plate Layout Position 2790 Layout Clear Plates i Plate Type Name Agilent54 Agilent54 Agilent54
14. k C _ Agilent27Eppendorf1500uL bet ed DVD Drive D GSP1 Agilent27Eppendorf2000uL t ANSIS6Well5O0uL tt Gia Network _ ANSAgilent54VialPlate1500uL te 21 Appendices B Importing Agilent Well Plate Definitions 5 In this example an Agilent 54 well plate will be selected Select the Agilent 5AVialPlate text file and click OK to import System Administrator Configuration Manager File Edit Wiew Records Tools Help Alaa a x ele rA Fess pear a Edtview Update MaxRows fioo fd a gt dil B eme ee Empower 3 Configuration a Projects 1 24 uCfuge tube 1 5mL ce E Nodes B Systems ae Libraries 384 well 80uL 48 uCfuge tube 0 5mL 48 vial holder 2mL 96 well tall 1mL 7 96 well tall 2mL 96 well tall 350uL meena ra em ee A H 96 well w 700uL insert ER Waters can import a plate type definition from a text file 96 well 300 uL Browse to or type in the path and name of the plate type file then enter the desired name of the new plate type definition Agilent54vialPlate PAORA E SL Plate Type Definition Import File ANSI 24V ial4mLHolder p fc Users locAdmin Desktop 4gilentPlatesF orlmport Empower 3 En US eCord 2 24 vial open access 2mL ANSI 364yvell250uL ANSI 48TubeO 65mLHolde 17 ANSI 48 vial2mLHolder Agilent54VialPlate z 2 _ ANSI 384yvell1 00uL New Plate Type Name 22 Appendices C Manu
15. nu and follow the steps provided on screen In the example above in the Samples tab the Sample Set Method table contains a series of parameters for making multiple injections 2 Ensure there are values for Plate Vial position injection volume the number of injections per vial Label and Sample Name in the Sample Set Method table In this example above 1 A 2 refers to sample position A2 being used in Plate 1 P1 3 Click on the green Run icon outlined above to start the analysis In Empower 3 1 A 2 corresponds to P1 A 2 in the Multisampler user interface For more information on plate configurations refer to the Appendix C Manual Set Up of Well Plates and Example Configuration Layouts for Standard Agilent Well Plates on page 23 and the Agilent 1200 Infinity Series Multisamplers User manual 12 Appendices Appendices A Configuring an Agilent LC in Empower These configuration steps have to be followed whenever a new module is added or removed Previously the old configuration has to be deleted from the DHCP server configuration Then the Empower software has to be shut down and the LAN connection to the module has to be switched off and on again When the LAN connection has been restored the new configuration procedure can be started Ensure that the module hosting the LAN connection is enabled to use DHCP This can be achieved by setting up the DIP switches of the module in a specific order Please refer
16. od Mew Method Set Open Method Set Instrument Method Mew Sample Set Method Template Open Sample Set Method Ternplate Amounts sample Set Info Dissolution GC Sample Set Info Molecular hdass Calculator Plates Alter Running sample ut Ctrl 4 Copy Ctrl C Paste Ctrl 4 2 Click the Multisampler tab The Instrument Method Editor is displayed with the default settings and the available Advanced features Enter the appropriate parameters in these fields and click the save icon to save the method 10 Multisampler Method Setup 3 Enter the Name Method Comments optional click Save and close the Instrument Method Editor Save current Instrument Wethad Names InstrMethM eT st lnstrhethod InstrhMethodh ew NewT esthfethod Mame Method Comments Save Cancel Help Start an Analysis in Empower 3 Start an Analysis in Empower 3 Ensure the Agilent well plate is positioned in the lowest drawer position of the Multisampler All positions in the Sample Hotel must be filled either with dummies or drawers The drawers must be installed from bottom to top Create a Single Injection Run To create a single injection run follow the steps below for creating a Method Set This can be performed from the main Empower Run Samples screen Infinity Din Infinity I as System Administrator Run Samples File Edit View Inject Actions Customize Help Is 4 t ED Sample Name
17. of the configured drawers and select the well vial plate matching the type previously set in defined plate within Empower Then click OK If the correct drawer configuration is not directly displayed in the LC Status Window after the Drawer Configuration Change either scan the instrument again or reboot the LAC E box The selected plates here must be the same type as in Define Plates configured within Empower Drawer Container ID Front 180 Rotated Container ID Back 180 Rotated OK Cancel Hep Defining and Assigning Plates in Waters Empower 10 Return to the Multisampler Instrument Status click on Modifiy click on Right Capillaries enter the appropriate settings for the Loop Capillary and the Seat Capillary and then click Assign E 0 20 Multisampler Method Setup Multisampler Method Setup This section describes the setup of the Multisamplers method and its various parameters once the LC system has been setup and configured in Empower It assumes you have completed the necessary steps to connect the modules and configured them within Empower See Appendix A Configuring an Agilent LC in Empower on page 13 for how to configure a Multisampler system It also assumes you have already imported defined and assigned the required Agilent well plate definition See the previous section E Infinity I in Infinity I as System Administrator Run Samples 5 E File Edit View Inject Actions Customize Help
18. ppendices A Configuring an Agilent LC in Empower 3 Drag and drop the desired instrument from the Available Instruments list to New System Instruments list and click Next gt System Administrator Configuration Manager File Edit View Records Tools Help alala 5 x e r a Fiter By Defaut 7 _Edtview _Update MaxRows 1000 fq gt ll E Fa Empower 3 Configuration Yai Node Name Node Type Node Comments SA m Ic ep3fr2 b1 Empower a Systems a ied Libraries Drag desired instruments from the Available Instruments list to the o New System Instruments list e Users Note You may open existing Systems and drag instruments to the ial QB User Groups New System Instruments list AGP User Types Available Instruments New System Instruments O Fiste types Unused Components m New System t System Audit Trall J Infinity2 QJ AgilentLC InfinityllDem i s Offline System Audit Trail BET AailentLC InfinitylIDem For Help press F1 4 In the Allowed Access dialog box select the radio button Owner Group and World Click Next gt System Administrator Configuration Manager File Edit View Records Tools Help Alaa oF x ele r E Fiter By Defaut 7 _Edtview Update MaxRows 1000 _ gt dil E Empower 3 Configuration Yai Node Name Node Type Owner Node Comments Poke a SE 1 ic ep3tr2 b1 Empower inn oo Systems Ee eA Libraries He eCord Share System with Oth
19. ppendix C Manual Set Up of Well Plates and Example Configuration Layouts for Standard Agilent Well Plates on page 23 Define Plates For Sample Set Method 2790 Layout Create New Plate Type Clear Plates Plate Sequencing Mode as Plate Type Mame Plate Layout Position 24 vial open access 2mL 384 well 80uL 45 uCtuge tube 0 5L 46 vial holder 2mL 96 well tall mb 96 yell tall 2mL 96 well tall S50uL 96 well w O0uUL insert 96 well 300 uL 9000000 OP OOOO 000000 000090 0000900 090090000 090000090 000006 0900000 Inject Standards kai Inzert The selected plates defined in the table above must be the same type and position as defined in the s Tray and Plate Configuration dialog of the Multisampler graphical user initerface refer to step 8 on page 6 Otherwise the mismatch prevents starting the analysis NOTE 3 Ensure that the just defined well vial plates for Empower matching the assigned well vial plates for the Multisamplers 4 Go to the instrument Status display Multicamplor gt Defining and Assigning Plates in Waters Empower 5 Right mouse click on the Instrument Status of the Multisampler to display the context sensitive menu Perform these steps only if you have changed the drawer configuration from last time i Control Se Method Injector Program Error Method Identity Device Home All Q Re
20. rail ANSI 24Vial4mLHolder ANSI 384well250uL 7 a a a E EAEAN E Import Plate Type From Text File 96 well vw O00uL insert ion 9 well w 700uL insert Waters can import a plate type definition from a text file 96 well 300 uL Browse to or type in the path and name of the plate type file a then enter the desired name of the new plate type definition Agilent54 vialPlate 12 ANAURA E O Plate Type Definition Import File ANSI 384yyell4 00uL c Users locAdmin Desktop AgilentPlatesFormport Empower 34En US ANSI 48TubeO 65mLHolda New Plate Type Name ANSI 48 Vial2mLHolder Agilent54VialPlate OK Cancel 4 The location of the Agilent well plate import files are part of the Waters Empower Driver pack currently located on the Empower Driver Pack CD in folder Agilent ICF Agilent ICF Support v 2 1 Hotfix 1 AgilentPlatesForlmport Empower 3 En US and take it from here Navigate to this folder using Explorer OU di gt AgilentPlatesForlmport Empower3 En US Organize v Include in library v Share with New folder WY Favorites ame MB Desktop ISHRVSmmlVialPlate tet b Downloads L ISHRV6miVialPlate tet Recent Places 15VialPlate bet 96DeepAgilent3 bt Libraries 96DeepAgilent4 tt Documents 96DeepRitter41 tt a Music __ 384Agilent bet Pictures L 384Corning bet Videos 384Greiner tt o 384Nunc tet jE Computer Agilent27Eppendorf500uL tt amp Local Dis
21. ration Manager o amp i File Edit Wiew Records Tools p E amp Empower 3 Configuration sy Node Type Node Comments si n Projects o lc ep3fr2 b1 Empower E i File Server Help The Waters DHCP Server is designed to work automatically without user IP Address MAC Address Type intervention but in some cases you will need to change or specify DHCP i settings for the instrument network or third party instruments in your 134 40 26 205 00 30 D3 EE FF 09 AgilentLC InfinityIIDemo laboratory Click the button below to configure third party Ethernet instruments on this node or to use a different IP address range for your instrument network Configure DHCP m Remove OK Cancel Help For Help pr 1 Total 5 Use Add IP Address screen to set the Instrument Type select Agilent LC from the drop down list and enter a unique name for the system in the Serial Number Unique Name field then click OK d System Administrator Configuration Manager o amp s File Edit View _Records Tools Help ai Node name Node Type Node Comments Ic ep3tr2 b1 Empower System E amp Empower 3 Configuration i B amp B Projects HN th Waters DHCP Server Configuration i File Server Help a The Waters DHCP Server is designed to work automatically without user IP Add Add IP Address i intervention but in some cases you will need to change or specify DHCP settings for the instrumen
22. rs Waters Instrument Control Software ICS is Waters Corporation adoption of the Agilent Instrument Control Framework for their data systems This guide is designed to assist you in setting up a Multisampler based method Additional appendices explain the process to perform additional sometimes optional configuration steps to use the Multisampler For more information on support refer to the Waters Release Note Prerequisites Instrument Prerequisites e Agilent 1290 Infinity II Multisampler G7167B or Agilent 1260 Infinity Multisampler G7167A with Firmware Rev D 06 60 or higher e Other Agilent LC modules in the LC system must meet or exceed the minimum firmware requirements specified by the 3rd party CDS software vendor and meets Agilent s firmware set firmware interoperability requirements http www chem agilent com _layouts agilent downloadFirmware aspx whid 79809 Software Prerequisites The operating system and Empower prerequisites are documented in the Waters Empower Instrument Control Release Notes for Agilent Instrument Control At minimum the following components are required e Waters Instrument Control version ICS ICS 2 1 Hotfix 716004653 including Agilent ICF Version A 02 03 DU1 HF2 Agilent ICF LC Driver Version A 02 11 SP1 72 Ensure that all prerequisites are met before proceeding to the next step The component versions can be verified in the operating systems Control Panel Program and Features Account
23. set Injector Switch Valve to Bypass switch off Tray Illumination Multisampler Auto clean Prime Modify lt gt Assign Wellplates Drawer Configuration Right Capillaries Reference Vial Array 6 Click Modify from the drop down menu list and select the Drawer Configuration option 7 Click the Start button Fad Setup or Change Drawer Configuration Before the drawer configuration can be started please make sure you have fulfilled the following prerequisites the sample workspace of the Multizampler is clear all plates sample containers have been removed from the drawers each drawer has two sample trays inserted all open drawers are closed and locked the Multigarnpler door is closed The Multisampler will run through its configuration procedure this may take some minutes Defining and Assigning Plates in Waters Empower NOTE NOTE 8 Right mouse click on Multisampler Instrument Status and click Assign Wellplates Injector Program Error Method Identify Device a Home All Reset Injector switch Valve to Bypass switch off Tray Illumination Auto clean Prime Modify l lt gt Assign Wellplates et Instrument Status Module Options Diagnostics Log This accesses the Tray and Plate Configuration of the Multisampler 9 Select Tray and Plate Configuration of Pl up to PX depending
24. set up e For installation please ensure the account being used has Administrator rights and privileges Defining and Assigning Plates in Waters Empower Defining and Assigning Plates in Waters Empower In order to use Agilent well plates with Empower and the Multisamplers well plate definitions for these plates must exist already in Empower If they are not present then you will need to import at least one Agilent plate definition file for the first time See Appendix B Importing Agilent Well Plate Definitions on page 19 for importing Agilent well plate definitions Proceed with the following steps to define the Agilent well plate within Empower 1 Go to the main Edit menu then click Plates Rod Infinity I in Infinity I as Systern4dministrator Run Samples File View Inject Actions Customize Help iR New Method Set Open Method Set ir Instrument Method Mew Sariple Set Method Template Open Sample Set Method Template Amounts sample Set Info Dissolution GC Sample Set Info Molecular Mass Calculator Plates Alter Running sarnple Cut Ctrl X Copy Ctrl C Paste Ctrl Defining and Assigning Plates in Waters Empower 2 Within a row of the Plate Type Name column click the drop down arrow and select the plate of interest If the plate type is not present it need either to be imported Appendix B Importing Agilent Well Plate Definitions on page 19 or to be created manually A
25. t network or third party instruments in your laboratory IP Address Click the button below to configure third party Ethernet instruments on this node or to use a different IP address range for your instrument MAC Address network Instrument Type Serial Number Unique Name Configure DHCP edit Remove OK OK Cancel Help For Help pre 1 Total The unique name defined here will then be used as the address in the Node Properties Instrument tab 14 Appendices A Configuring an Agilent LC in Empower 6 From the main menu navigate to the Node Properties Instrument tab and click the Scan Instruments button to check you have a connected instrument General Instruments Serial Parts Configure DHCP Access Me e oe _ eects i 1 AgilentLe AgientLcaintinityiDema zi mo O C e r Scan Instruments Remove Inetrument Cancel Help From the example LC Status Windows information view accessible via in the LC status window you can review the list of connected LC modules and related information as shown Vendor Name Part Number Serialnumber Firmware Revision Connection Info Driver Version Additional Information Agilent Binary Pump G7120A DEBA200149 B 06 73 0001 134 40 26 205 A 02 11 SI 517 05 Agilent Column Comp G71168 DEBA400197 D 06 72 0002 134 40 26 205 A 02 11 SI 517 05 Slave Firmware C 06 72 0001 Agilent DAD G71178 DEBAWO0171 D 06 72
26. tr2 b1 my second instrument g Libraries H Cord ae User Groups AG User Types BP Plate Types en System Audit Trail NOTE You can only run an analysis when OnLine is set as Yes in the table above 18 Appendices B Importing Agilent Well Plate Definitions In order to use Agilent well plates in conjunction with Empower 3 and a Multisampler well plate definitions must exist already These steps outline the procedure for importing new Agilent well plate definition files into Empower If the Agilent well plate types are not present in the list it will be necessary to search and import at least the Agilent 54 well plate definition txt file as this is not installed automatically by Empower 3 Also importing of plate definition txt files can only be performed one at a time From the Configuration Manager window select Plate Types in the left navigation pane to display the current list of well plate types available that can be used within Empower Determine if the Agilent well plate types are present In the example the plates do not exist so follow the steps below 7 System Administrator Configuration Manager File Edit Wiew Records Tools Help Aaa l x Pele E Filter By Defaut _Edtview Spe Empower 3 Configuration WB Plate TypeName Projects pan po Nodes Systems oes ecard ece o sewatwreou r He User Groups ot ET User Types AD Plate Types AMSI 24vialdimLHolder
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