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Incubators User Manual

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1. e ui 8 5 JAN 5 OcOvvc XIV 09173 vi 9 3911 3 021115 02077 2 aIv 03113 El AOSNAS 1 781080 STSHS 1 12 799061 IT 801 1 1 amp 0 0061 01 ATAWASSV 8NS LINO 401 SATSAS OLE 150061 6 31 14 SS322V 4800081 8 8 831713 VId3H 3181509510 100044 VId3H 005 5 SL108Z 9 Vd ALIOIWIH SIOLEC 5 AVE 82 571995 SLlvcc v 19995 1210 C M AV INCOW 21051 QNTA O 01115 SS 02 1 700 2 1 ALO NDI1dIS2S3U 18 ON Lavd d SivIa3lVW JU 118 S3INSS 0008 01 NOWWOD 0300 522 grg 0 22 10 eSz N1 v SLON 0300 aSW grg 90 0 90 90 781062 01 38084 1062 Q3ONVHJ NOT NP 0 21 z 1334S LOTO 58 1 30 01 30 820 10 91 50 1 303 ISYA 820 220 1071177 V N 0 5 30 14140530 2 48 N33 8 E 002 0LE DNO 8 3 Direct Heat CO2 Incubator Thermo Fisher Scientific
2. 1 002 865 Ie cwm SINY A oaa 719150317319 a een SINVAS TOL YN 80702 INIVd YN 5044 3WOS 90 0 90 31 0 Ogay 909 0v3 479 815873 OWASHL 5 5 SNTANLIVANNVW 404 0 51 ATAWASSV 13738 TANYd 10 1 09 9MQ AON ANY 803 SHSHLO OL 03507510 38 UL LON SI HINS SOLVENINI 531935 IWYN 18Yd l300N 5 1 SIHI te 02 Ll 91 51 553998 1904 762061 71 01 7 1 X 00 1 I Ovi El amp gHSVM SS 00 829 X QI 917 0900 4 9 4 55498 1 8 1 X JSDH 11 3 MAIS SWIS dHd v I X 2 4 20065 01 9319909 5528 193 8 1 X JSDH v I 590078 6 ASHSWM 1 14 SS B E 8 13388 1904 3144 5 SGE0081 L HILIMS 8000 931 JILSNOVW 0 209 9 Vcl HILIMS 3 388 1115915 08102 5 GavO8 11 810 081 09 v HJLIMS 3700 18815 9209 I TANVd 109102 1420061 2 ANYA 081 09 L8EOvI 1 114182530 1avd B3BWIN lavd WALT 30 1118 01 NOWWOD 33 979 0 61 10 6SLE NI 1 NOILINGOSd 903 C3SY3 38 BSH 979 5
3. pue 104 syzuow xis jeuonippe ue Jno 2016 oym sJejeeq 94 Buunp saumo Aue UO 94 si noA uenem y OS siu ano 5 si y sujuoul SES SUL 41193 21311331965 3HSI3 OWYSHL Product Description Product Designations Year of Initial Marking CE Affected Serial Numbers Release 1 Release Level REL shown on senal tag This product conforms to the following European Union Directive s EMC LVD B9 336 EEC T323 EEC This product conforms to the following Harmonized Intemational and National Standards 61326 1 1997 50081 1 EN 50082 1 EN 50011 1 97 LVD EN 61010 1 1993 Amendments 1 and 2 CSA C22 2 No 1010 1 UL 61010 1 UL 61010 2 010 Regulatory Compliance Manager Fisher SCIENTIFIC 25 January 2007 eclaration uf anfurmitg Manufacturer s Name Manufacturer s Address Product Description Product Designations Year of Initial Marking CE Affected Serial Numbers Therm
4. 6 1 DeinoteA DENIS 6 1 Humidity Readout 225524 hows 6 2 Section Specifications 7 1 Section8 Spare Parts 8 1 Exploded Part Drawitpgs e dies ebat ik Re Ra RE PERSE RE C 8 2 Section9 Electrical Schematics 9 1 Section 10 Warranty Information 10 1 Section 11 Declaration of Conformity 11 1 Thermo Fisher Scientific Direct Heat C02 Incubator viii Section 1 Installation and Start Up Chamber Gas Sample Port Control Panel gt 5 Sterilization Switch BH eS Cycle Button See 5 Section 1 Inner Door lt 2 Leveling lt Legs 4 Door Heater See Section 5 Cable Figure 1 1 Components Chamber Gas Sample Port Used for sampling chamber content using a FYRITE or similar instrument Main Power Switch Mains Disconnect Control Panel Keypad displays amp indicators Figure 1 2 Leveling Legs Used to level the unit Sterilization Cycle Button Switch to initiate sterilization cycle Note The incubators are stackable Information follows Thermo Fisher Scientific Direct Heat C
5. W 72 73 74 75 76 38 4 RJ 11 21 25 r h J1 4 m tjl JI 5 J1 6 com 1 7 J10 lt RS 485 RS 485 TEMP _ OUTPUT BOARD ANALOG C02 re RH gt 5 413 3 113 4 13 5 gt 13 6 8 7 J7 1 Jig 47 6 47 3 2177 7 5 Q 048 Ht REMOTE ALARM CONNECTIONS shown in alarn state OPTIONAL RS 485 OUTPUT KIT 1900085 OPTIONAL RH SENSOR KIT 1900091 OPTIONAL ANALOG OUTPUT KITS 190512 4 to 20mA 190543 0 to SVDC 190544 0 to 1VDC OPTIONAL GAS GUARD KIT 1900086 C02 PRESSURE SWITCH 41 TANK 360213 PRESSURE SWITCH 42 TANK EMPTY 360213 SULENUID 3 WAY 12VDC 250121 MODEL 381 IR 02 SYSTEM 231204 Electrical Schenatic Model Direct Heat Incubotor with Sterilization 371 70 0 0 REV 4 Page 2 of 3 9 5 Steri Cycle CO Incubator Thermo Electron Corporation Section 9 Electrical Schematics Thermo Electron Corporation 77 79 WIRE REFERENCE CHART GA COLOR
6. WATER Figure 5 14 Displays with IR Sensor Removed Direct Heat CO2 Incubator 5 9 Section 5 Routine Maintenance 6 After ENTER is pressed the Heat Phase initiates The white LED flashes and the display toggles between STERILIZING and HEAT PHASE During this period the HEAT light will be on and the cabinet heats to sterilization temperature AUN SET CAL CON o o o STERILIZING ss TN RUN SET CAL N 5 o HEAT PHASE 8 Figure 5 15 Heat Phase 7 When the air temperature in the unit reaches 140 0 C the Sterilization Phase begins and the display changes to STERILIZING SET CAL 1 STERILIZING CO Figure 5 16 Sterilization Phase 8 After approximately 2 hours a 5 second audible tone sounds signaling that sterilization is complete The Cool Phase begins The display toggles between STERILIZING and COOL PHASE AUN SET CAL CON o 5 STERILIZING v AUN SET CAL CON COOL PHASE Figure 5 17 Cool Phase 5 10 Direct Heat C02 Incubator Thermo Fisher Scientific Thermo Fisher Scientific Section 5 Routine Maintenance 9 Completed Sterilization Cycle with T C sensor When the temperature cools down
7. 007 fanr 8 00 0 Toc UNHDED STENY EvGE ZVGE SE OvGE B6GE OL NOANDO 5 SAWIN 091512395 3SINN3HID SSINN INVANO n g m NGSYG3US NOISSINdd ale 370 ato 80 00 LEN 3011 OND 3 SIDES OLE CIN SNIVIND 1NGNTTDO 5 25 SNMLVSIANSL OLE 98106 01 MBWSSSY 1809 55823 22001 31 14 001 SSYD 060061 8 33988 __1110061 Yaldvoy 55 38 2800051 9 2 LL 5 1431 SS Oz w 1 56 009 v 2 Oz 7 Saves Ose e 990 21 611001 2 v IFN ONIM cE B4 _ 15062 ALO 14 20930 Lave ON ary 30 1118 531435 0008 01 0 590 9 9 9 0 22 10 GSLE NI Z T431UN 0300Y 9 9 9 9 90 0 90 SOEE NI NOT19NGOsd and 03597384 820 820 0 1177 0 51 30 114180530 dddv OVI A8 3170 ON N23 38 8 7 002 0 33840N KO Section 8 Spare Parts Thermo Fisher Scientific Direct Heat CO2 Incubator 8 4 Section 8 Spare Parts Tuas OIHO V1L3INVM 699 XOG 8 5 002 01 lt
8. e uo pue Surueo poys oq J Suruonoung yun 1no doox 3 sr sanud poie1qi e pue 4 sey 1usuidmbo 51018411241 JOINVNILNIVIN se 2242 1215 se 10 TOD pue say o dures 3104 552222 Vedy sunnow 296 101eqnour JO 10129101 IY VULI 226 se uonedqipeo pue pur doi 1 24 ued ou ur YoY Teas 2958 100p pue 100p JULI OO IPD X 5 996 ppuo A p M Aea uonoy gt 8 59199 10 Thermo Fisher Scientific Section5 Routine Maintenance Warning If the unit has been in service turn it off and disconnect the power cord connector before proceeding with any maintenance A Caution Before using any cleaning method except those recommended by the manufactu
9. 1130 8319NV 3215 ONIMVYO 031319345 JS1MU3HIO SSIINN 12Nv81101 2 1411 3125 LE O 13125 10 92 31 0 044 820 0 2 NAQ 213119319 919513 ONUJHL NOYA NOISSINU3d NILIIYA INOHLIM 3504804 INIYNLIVANNYW 401 0381 A18N3SSV TINVd WOVE OLE 31111 OMQ YON 1504804 ANY 404 SYIHLO 01 435012510 38 01 LON 1 NOIIVNUOJNI HONS NOILVNUOJNI HOLVGNINI OLE 3VN 18 4 1300 AYVLIIYdOYd SNIVLNOD IN3N 2OQ SIHL 914 GHNTG 11 GLE 81502008 OHNI INN 616 0 8 ONILLI4 052 9147020078 8 OLE 3801 LINI TANIA 100S6 _1 13INI 200 O1E 9 ONIGNL ANIA 917 GIOZL 2 130NI 20 01 TANYA WVA 1 0061 349105 51 1006 4314904 ONIGML 2615 800078 vil 831113 1181504510 100011 Gl SLE U3HSVA 83011095 7001 2157 4 Uadd NS 90009 GLE 4 12 UIda NS 0009 081097 131110 3008 851097 JLIHM 131100 NI a NS 720097 1083105 580052 KINO 0861015 13877 200 995022 138 58 595022 T38Y1 TONY 01 83338 060022 T38V1 LOVINOD NAYI 86022 118 1 1310 55322 552022 INNON 131100 14055322 766061 NO LIE VIR 9119 3108 NI dYNS 2 1 UIHSVANDOT HLOOL 112 9t 85062 WINSVA 1 12 8 E CAINO 0957048 HSVMNT 42 26 98 10062 83425 8 6 X 2 9 6702 MANOS 2 26 9 11022 NO11d182630 18 4 ON
10. 30 11 13 4 XOVG 083 0015 10 21 21 1295 11 531815 008 Ol NOWNOD 0300 10 22 10 1 2108 0300Y 90 05 90 9066 025 OL JAWA QUVddl 19 680062 0 90 70 61 50 1816 1 131100 999138 10 62 80 620 414 NOISIA3U 10 0114182530 3100 6 66 OSE SESE OL 8 5 002 016 U38N N OMQ 8 5 Direct Heat C02 Incubator ientific Thermo Fisher Sc Section 8 Spare Parts Tuae 8 9 002 01 TIC 1081230 S319NV 3215 031319345 ISIMYIHLO SSIINN 32NYU1101 Y N OUWIU3IVN 05157 40 quaudinb3 uswuos aug pa 02ju05 011 04802 812313 00670 31 25 10 82 31V0 NOT QddV 820 0 2 37 0 NAO 0419313 WOUJ 80155148434 N3LLIUM IMOHLIM 1504804 ONIYMLIVANNYW 303 0350 ASSY 13NYd 1081502 331111 OMG YON 350484 ANY 403 Su3HIO OL 035012510 38 01 LON SI 531835 OLE 3WVN luvd 1300H AYVLIIYdOUd SNIVINOO SIHI NOILVNUOJNI HONS ONY KIGNISS GNS AVUL INJNOGNOO
11. COLOR ND GA COLOR 79 6 2 18 reo 42 22 3 BLACK 80 2 6 BLUE 22 18 GREE 43 22 3 RED 3 6 GRN YEL 23 18 ORANGE 44 22 3 GREEN 8 4 6 BLAC 24 18 GREE 45 22 BLACK 82 Al 6 WHITE 25 22 BLUE 46 20 RED 8 BLAC 26 22 BLUE 47 20 RED 7 6 YELLOW 27 22 BROW 48 20 BLUE 84 8 8 ORANGE 28 22 BROW 49 20 BLUE 95 9 8 RED 29 22 YELLOW 50 20 ORANGE 0 8 BLUE 30 22 YELLOW 51 20 ORANGE 86 1 18 REO 31 22 RED 52 20 BLACK Q7 2 DE exe 32 22 RED 53 18 BROWN 3 33 22 GREE 54 24 4 BLACK 88 4 Bs 34 22 GRAY 55 24 4 WHITE 89 5 EI 35 22 PURPLE 56 24 BLACK 90 6 Sess 36 22 ORANGE 57 24 4 RED 7 18 ORANGE 37 22 3 BLACI 58 24 4 GREEN 81 8 18 BLACK 38 22 3 RED 58 92 9 18 YELLOW 39 22 3 WHITE 60 20 40 22 2 RED 93 41 22 2 BLAC 94 95 96 97 98 98 00 01 02 03 04 MODEL DESCRIPTION 3541 STERI CYCLE TC 02 INC 230V 05 3543 STERI CYCLE CO2 INC 230V 06 07 4 IN 3759 01 22 07 GJG CCS ADDED 8000 SERIES MODELS y 08 04 03 AN owe 200137 eee 20018 Electrical Schematic 2 2970 09 04 01 GLM KOG LON 096 HARNESS FOR MICRO SENSE VOLTAGE LOSS Mode 1 2915 04 02 01 ONF CHG 130625 FAN 900135 amp REV FLA 0 VA 1 23 01 ove RELEASED FOR PRODUCTION REV DATE cad PPO DESCRIPTION OF REVISION Direct Heat
12. ON Qaridii IVYO YSHLSHM SSILNVYYVM YSHLO 30 NI ANY SI ALNVYYVM SIHL uoneunsep 804 peddius sued pue pied peuunjeJ eq 1snuu sued uodo sowy y Aue Jo uunjeJ JO seoruesg eoiuuo9 eu poled Ajueuew y y JO y JOYE JOU eys siy Jepun Jo sued JO SIU pue ssej6 sway ejqgepuedx3 Aue pue pejoejuoo eq jueunjede 9 4 29 siy JOU pue 5 peoe dau Jo eq JO Buruuojuoo uou ue oud sued jee ysa Bung pue si
13. Front mounted barbed Electrical 115 Volt models TU 115VAC 50 60 Hz 1PH 9 6 FLA Operating range 90 125VAC 230 Volt models 230VAC 50 60 Hz 1 4 4 FLA Operating Range 180 250VAC Power switch circuit breaker 2 Pole 12 0 Amp Accessory outlet Voltage equal to the cabinet input 75 Watts maximum 0 5ma leakage current Alarm contacts Deviation of temperature CO power NO and NC Thermo Fisher Scientific Section 7 Specifications Dimensions 26 1 W x 39 5 H x 25 0 F B 66 100 63 5 Interior 21 3 W x 26 8 x 20 0 F B 54 0cm 68 1cm 50 8cm Weight Ae Ged s 260 Ibs 118kg Net shipping 315 lbs 143kg Certifications Refer to the Declaration of Conformity at the end of this manual Safety Specifications Indoor Use Only AGE anh eaten Uis 2 000 meters Temperature 5 to 40 C 80 RH at or below 31 C decreasing linearly to 50 RH at 40 C Mains Supply Fluctuations Mains supply voltage fluctuations not to exceed 10 of nominal voltage Installation Category Pollution Degree 2 Class of Equipment 1 Installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvo
14. U3HSYM 1 02 1115 01 69005 30 LYVd OLE NOILVINSNI 93 019 614 00707 9NIHSQB L4VHS 483 019 6180061 LINNON NO 0108 1131 2080061 110051 YAWYOISNVYL V Ay 701027 1 1S0N 051007 YOLOW 3019 111961 LAN ONIM S S 2 16082 0114182530 1874 S1VId31VA 30 1 ON 1974 po 531935 0008 04 01 NOWWOD 03007 522 ro zz 1o 2 13108 Gon sw oro oro eocen NOISIA3H 10 0114182530 444 0 2 28 ou 8 2 002 016 1 38 tvSE ZyGE IpGE OpGe 966 OL I Thermo Fisher Scientific Direct Heat C02 Incubator 8 2 Section 8 Spare Parts FEXXX 05 lt vlii3lavW 9 X08 8 E 00 0E FXX WATTS ISTNY Js SIWON SNTAVAO 091312345 ISINAHIO 5537 1 JI4ILNAIDS 2 1 OL 1 JeusiJouueu 1 PEN CHUA 813 NULSSIRE 020 35 avo oia wo wo ND AN AN 04 SHLD 0957105 38 DI LON SE HTS MOON 7207 19815 SETS iN Le BOOK SNTVIND
15. safety to Setpoint protect the incubator only It is not intended to protect or limit the maximum temperature of the cell cultures or customer s equipment inside the incubator if an overtemp condition occurs Series 8000DH incubators are equipped with a secondary temperature monitoring system to monitor the air temperature inside the cabinet This system is designed as a safety device to turn off all heaters in the event of a temperature control failure Temperature control in the incubator will be 1 of the overtemp setpoint The overtemperature is set by the factory default at 40 C However the overtemp can be set up to 55 C in 0 5 increments If the incubator operating temperature setpoint is set above the overtemp setpoint the overtemp setpoint will automatically update to 1 C above the temperature setpoint It is recommended that the overtemp setpoint be maintained at 1 C over the operating temperature setpoint Thermo Fisher Scientific Direct Heat CO2 Incubator 1 13 Section 1 Installation and Start Up 1 14 Setting the Overtemp Setpoint continued Setting the Setpoint Direct Heat CO2 Incubator To set the Overtemp setpoint 1 Press the MODE key until the SET indicator lights 2 Press the right arrow until Otemp XX X is displayed in the message display 3 Press the up or down arrow key until the desired Overtemp setpoint is displayed 4 Press ENTER to save the setting 5 Press the MODE
16. Press Enter to save the setting Display temperature OFF Display humidity ON Press Enter to save the setting Display humidity OFF Tank Select Press lt to return to previous parameter TANK SEL X Tank 1 prime Press Enter to save the setting Tank 2 prime Gas Guard 15485 Press toreturn to previous parameter Press lt q toreturn to previous parameter GASGRD XXX RS 485 XX SCROLL FOR PARAMETERS Gas Guard ON Press Enter to save the setting Gas Guard OFF Numbers increase Press Enter to save the RS485 address Numbers decrease 3 10 Direct Heat CO2 Incubator Thermo Fisher Scientific Section 4 Alarms The Series 8000DH incubator alarm system is shown in the table below When an alarm is active the message appears in the LED message display Pressing 05 disables the audible alarm for the ringback period However the visual alarm continues until the incubator returns to a normal condition The alarms are momentary alarms only When an alarm condition occurs and then returns to normal the incubator automatically clears the alarm condition and the message display Table 4 1 Alarm and Description No alarm condition exists SYSTEM OK or CLASS 100 eel Temp gt Otemp Set point SYS IN OTEMP Air Temp Sensor Fault See Section 4 AIR SNSR ERR 02 Sensor Fault See Section 4 02 SNSR ERR Replace HEPA filter reminder set time expired REPLACE HEP
17. 3 3 Enable Low Temp Alarm to Trip Contacts 3 3 Set Low Alarm Limit tracking alarm 3 4 Set High Alarm Limit tracking alarm 3 4 Enable Alarms to Trip Contacts 3 5 Set New Zero Number for T C Sensors 3 5 Set New Span Number for T C Sensors 3 6 Setting a Low RH Alarm 1 3 6 Enable RH Alarms to Trip Contacts 3 7 Enabling Temp RH to be Displayed 3 7 Sections 4 1 Temp Controller TMP CNTR ERR 4 2 Sensor Fault 4 2 Preventive Maintenance 4 3 Section5 Routine Maintenance 5 1 Clean Cabinet Exteriot ooa rej re xe nse 5 2 Clean Glass 5 3 Clean Humidity Pan eaa a eae E eee Roxen see 5 3 HEPA Filter Maintenance 5 5 Replace Power Fuses ese PEOR 5 5 Replace Air Sample 5 6 bare 5 6 Sterilization Cycle deka 5 8 Direct Heat CO2 Incubator Thermo Fisher Scientific Table of Contents Section6 Factory Options
18. Humidity pan natural vaporization Humidity w 95 RH at 37 C Display sot ettet t Optional in 196 increments Alarm Low RH with optional RH monitor CO Control 0 1 microprocessor PID control GERSON or IR Beadability qu arty nana RE 0 196 RANGE det o Resa kx tru 0 to 2096 Inlet pressure 15 psig 1 Display Digital LED 0 1 increments Direct Heat C02 Incubator Specifications are based on a nominal voltage of 115V or 230V in ambients of 22 C 7 1 Section 7 Specifications 7 2 Direct Heat C02 Incubator Shelves Dimensions 18 5 x 18 5 47cm x 47cm Construction Stainless steel belt sanded both sides Surface area 2 4 sq ft 0 22 sq m per shelf Max per chamber 38 4 sq ft 3 6 sq m Loading 35 lbs 16kg slide and out 50 lbs 23kg stationary 2225 suus bud ba SRO aout 4 d ERES 16 Construction Interior volume 6 5 cu ft 184 liter Interior Type 304 stainless steel shiny finish Exterior eos 18 gauge cold roll steel Outer door gasket Four sided molded magnetic Santoprene Inner door Bulb silicone Insulation eos Mineral wool Fittings Access port 1 1 4 32mm removable silicone plug CO inlet ise cheated 1 4 6 4mm barbed Sample
19. 100044 vc c 8 a3HSVM TWAS 3NDDI VIS 00 2 1 98006 1 SSO TIDHS ELOOEL c 00 0 X B3HSvM JAVA LSOOEL 1 13 SS 01 582 9500 0c JOIXATA SS 26 8 640019 61 1 895 4 11 5 1 1 1 X ZE 8 550055 c 13305 SdITIHd 55 X ZE 8 750055 1 9 13805 241 X 02 050055 91 1 MJIS SS 2 1 X 07 7 920067 SI 1 HILIAS vi amp 11 99 45 220521 1 117130 80NA dA3L 24021 cl c 1HDI3H 39NIH 69 39NIH 55 601911 1 55419 4 EZ X 5 8 92 X 5 02 990501 01 191579 000 JINNI 511 01 8 1 241 7 LLOOE 8 v ONIM 3535 2E 8 150 2 L SS B3HSVM 2207 TWNASLNI 9 0 0 8 2 LV 3 SS 8 0 2 5 e 9895 SS 1 X ZE 9 1144 v 5 MAMIS SS B E X ZE 8 50 Ol 13805 SS 2 84 15022 c v 13805 SS X 00 1 85002 1 114142530 Lavd ON avd E S JU 118 lentitic Thermo Fisher Sc Steri Cycle CO Incubator 8 10 Section 9 Electrical Schematics POWER CONNECTION 115VAC 50 60HZ 1PH 9 6 FL
20. 6 ONIHSAG d NS 8 1 88005 ONE 4015 51 120061 8 2 43825 9931 2 1 X 8 0 0vc TY HSYMYT dZ 29 89 20062 2 SY 3114 8 21 OLE 210061 gt ONIY 9NINIVUIS3U 5 401138 8110061 9 v 3925 SS P E X 2E 8 6102 f ON LNAOW 21 3NO310 4 10d 2800061 5 NOILdIYOS30 1809 ON Hd 405836 OL dWGI IB 891064 STVIYSLYW 10 1118 801 031010AN02 eller 5 IN HSVMW dZ 9 10062 2 521815 0008 01 NOWNOD 1300 10 22 10 6cie N1 v dd SS 062 1 26 9 12122 3108 0300Y 90 08 90 ON UV Id U31V3H 2610061 0300 805825 21 vor 10 12 60 1962 ALO NOI1dIN2S30 1399 ON 199 yn YOSN3S 21 01 SINN ONIY 8110061 4300Y 00x 10 90 10 c162 NI 10 0084 0393138 11 820 820 10 72 00 vn 0 SIVIYSLYN 30 1119 NOISIAIY 30 14182930 aaav avo 3190 8 8 002 01 U38NDN OMG Section 8 Spare Parts lentitic Thermo Fisher Sc Direct Heat CO2 Incubator 8 8 Section 8 Spare Parts LL bw ea gt N 1 aAa C Lr m d m o i d gt m 1 S co gt LII AL C7 22 C m Ll ca Thermo Fisher Scientific Steri Cycle CO Incubator 8 9 Sectio
21. IR sensor If an IR sensor is connected the display toggles between POWER OFF and REMOVE If power is not turned off within 1 minute the display returns to SYSTEM OR Thermo Fisher Scientific Thermo Fisher Scientific Section 5 Routine Maintenance Note To remove the IR sensor if applicable first power the unit off Then turn the two 1 4 turn fasteners on the top duct until the cover plate is disengaged Secured on the inside of the plate is a dummy sensor Figure 5 6 NS on inside Figure 5 6 Dummy Sensor Figure 5 7 Sensor with Cable Grip the IR sensor and carefully pull it downward Unscrew the cable from IR sensor Figure 5 13 Set the sensor aside See Step 9 for the IR sensor disinfection procedure Connect the dummy sensor to the cable Fit the installed dummy sensor up into the previous sensor location When the power is turned on with the IR sensor removed the display toggles between REMOVE HEPAs REMOVE WATER and PRESS ENTER If ENTER is not pressed within 1 minute the display toggles between POWER OFF and REPLACE IR When the unit is powered back on with the IR sensor reinstalled the display returns to SYSTEM AUN SET CAL CON o o RUN SET CAL CON PRESS ENTER AUN SET CAL CON o o o
22. REV 5 Page 2 of 3 Direct Heat CO2 Incubator Thermo Fisher Scientific Section 9 Electrical Schematics 77 78 WIRE REFERENCE CHART 79 ca couo 5 BROWN 21 18 REO 42 22 3 BLACK 80 2 5 BLUE 22 18 GREE 43 22 3 RED 81 3 6 GRN YEL 23 18 ORANGE 44 22 3 GREEN 4 BLACK 24 18 GREE 45 22 BLACK 82 5 6 WHITE 25 22 BLUE 46 20 RED 83 5 8 Back 26 22 BLUE 47 20 RED 7 5 YELDW 27 22 Brow 48 20 BLUE 84 g 8 ORANGE 28 22 BROW 49 20 BLUE 85 8 8 RED 29 22 YELLOW 50 20 ORANGE 96 0 8 BLUE 30 22 YELUW 51 20 ORANGE 8 RED 31 22 RED 52 20 BLACK 87 2 32 22 RED 53 18 BROWN 88 33 22 GREE 54 24 4 BLACK 2 34 22 GRAY 55 24 4 WHITE 89 5 35 22 purPLE 56 24 BLACK 90 5 e 36 22 ORANGE 57 24 4 RE 7 18 ORANGE 37 22 3 BLACK 58 24 4 GREEN 8 BLACK 38 22 3 RED 59 92 9 18 YeLLoWw 39 22 3 WHITE 60 93 20 oes 40 22 2 RED 41 22 2 BLACK 94 95 96 Q7 98 59 MODEL DESCRIPTION 370 STERI CYCLE T C CO2 INCUBATOR 380 STERI CYCLE I R CO2 INCUBATOR 3598 STERI CYCLE CO2 INCUBATOR 3599 STE
23. cm or a conductivity range of 20 0 to 1 0 uS cm Refer to ASTM Standard D5391 93 or D4195 88 for measuring water purity Distillation systems as well as some types of reverse osmosis water purity systems can produce water in the quality range specified Tap water is not recommended as it may contain chlorine which can deteriorate the stainless steel Tap water may also have a high mineral content which would produce a build up of scale in the pan High purity or ultra pure water is not recommended as it is an extremely aggressive solvent and will deteriorate the stainless steel Thermo Fisher Scientific Filling the Humidity Pan continued Connecting the CO Thermo Fisher Scientific Gas Supply Section 1 Installation and Start Up High purity water has a resistance of above 1M to 18M Ohm Even high purity water can contain bacteria and organic contaminants Water should always be sterilized or treated with a decontaminant safe for use with stainless steel as well as safe for the product prior to being introduced into the humidity pan Check the level and change the water frequently to avoid contamination Do not allow the water level to fluctuate significantly Dry outs will have an adverse effect on the humidity level and calibration of the T C units Caution Use of chlorinated water or decontamination products containing chlorine will deteriorate the stainless steel and cause rust voiding the warrant
24. do not leave both exterior doors open at the same time Warning If the units have been in operation turn them both off and disconnect the power source before beginning any service work Two stacking brackets shown at left are included the parts bag shipped with each incubator Figure 1 3 Stacking Brackets 1 Remove the cover plate securing the door cord from the incubator to be on top See Figure 1 4 Disconnect the plug from the connector 2 Remove the four screws securing the door hinges to the unit Remove the door and set it aside Cover plate and bolt hole with door connector 19 Figure 1 4 Cover Plate Figure 1 5 Hole Plug Locations 3 Unscrew the two hole plugs from the top cover of the incubator to be the bottom of the stack Figure 1 5 2222 ____ 4 Unscrew and remove the 4 leveling feet from the unit to be stacked on top and lift it onto the bottom unit Align all sides Warning This incubator weighs 260 lbs 118kg Have sufficient personnel available when lifting Lift the unit by the sides of the cabinet base to avoid placing stress on the outer door hinge Figure 1 6 Bolt Holes Direct Heat CO2 Incubator 1 5 Section 1 Installation and Start Up Stacking the Incubators cont Preliminary Cleaning 1 6 Direct Heat C02 Incubator 5 Insert the stacking brackets into the slots at the back of the stacked units as shown in Figur
25. este do eee 1 3 Displays 1 4 Installing the Incubator La ux Lee viet ER Eu ate ee 1 4 Stacking the Incubators 15 een sue Ri eR ERR S Erbe ed 1 5 Preliminary Cleaning 243 rb o Rr ER by ed 1 6 Shelf installation cu e Fart rem Ecrit iot 1 7 Access Port Filter amp CO Sensor Cover Plate 1 8 Air Sample Filter Installati h eaten oes e tee 1 8 HEPA Filter Installation Drs ae ded e 1 9 Leyeling the Unit Loss taaie dbi vn ed 1 9 Electrical Power 1 9 Filling the Humidity oes dere eiei ene taea uiie 1 10 Connecting the Gas Supply 1 11 Incubator Start Up occa acia oat ee ons 1 12 Calibration MeMT 2 1 Tempeiatute a 2 1 Thermal Conductivity CO2 System 2 2 Infrared Systane Gg o UU Neb da iE E Ha a 2 3 Relative Sa acies deret de edad d 2 4 Direct Heat C02 Incubator vi Table of Contents Section3 Configuration 3 1 Turn Audible Alarm ON OFF 3 1 New HEPA aa 3 1 Set REPLACE HEPA filter reminder 3 2 Set Access Code sna E Ea e 3 2 Set Low Temp Alarm Limit tracking
26. fill your needs for spare or replacement parts or provide you with on site service We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products Whatever Thermo Scientific products you need or use we will be happy to discuss your applications If you are experiencing technical problems working together we will help you locate the problem and chances are correct it yourself over the telephone without a service call When more extensive service is necessary we will assist you with direct factory trained technicians or a qualified service organization for on the spot repair If your service need is covered by the warranty we will arrange for the unit to be repaired at our expense and to your satisfaction Regardless of your needs our professional telephone technicians are available to assist you Monday through Friday from 8 00 a m to 6 00 p m Eastern Time Please contact us by telephone or fax If you wish to write our mailing address is Thermo Scientific Controlled Environment Equipment 401 Millcreek Road Box 649 Marietta OH 45750 International customers please contact your local Thermo Scientific distributor iV Direct Heat C02 Incubator Thermo Fisher Scientific Preface Warranty Notes Information You Should Know Before Requesting Warranty Service Locate the model and serial numbers A serial tag is located on the unit itself For equipment service or mainte
27. s RH 12 5 CYC COMPLETE INJ 4 A RUN SET CAL 30 0 N RH 1 K REPLACE HEPA INJ CO 4 9 0 v RUN CRL CON WERT 30 0 E RH UI 48 PRESS ENTER INJ CO 4 y Figure 5 18 Sensor Reinstalled Direct Heat C02 Incubator 5 11 Section 5 Routine Maintenance 5 12 Direct Heat CO2 Incubator 10 Open the chamber and install a new HEPA filter air sample filter and access port filter assembly Press ENTER 11 When ENTER is pressed the LED goes out the display returns to SYSTEM and the HEPA filter replacement timer is reset 12 Fill the humidity pan with 3 liters of sterile distilled water with the recommended purity range See Section 1 13 Allow to stabilize for at least 12 hours at the required temperature and level It is recommended that the temperature and calibrations be verified periodically during the first week of returning the unit to service Caution The high temperature sterilization cycle may cause the T C sensor output to change significantly This is normal and does not indicate damage to the sensor Therefore it is essential that the CO2 calibration is verified before returning the unit to service A If an independent instrument is not available to verify calibration the following procedure may be performed After the Sterili
28. to the original set operating temperature or 30 C whichever is higher the display toggles between CYC COMPLETE REPL HEPAs and PRESS ENTER The white LED is lighted but no longer flashes Completed Sterilization Cycle with IR sensor When the temperature cools down to the original set operating temperature or 30 C whichever is higher the display toggles between CYC COMPLETE POWER OFF and REPLACE IR The white LED is lighted but no longer flashes Clean the IR sensor with either isopropanol or Lysol No Rinse Sanitizer When using isopropanol simply spray the sensor do not saturate and allow to dry With Lysol spray the sensor do not saturate and allow to sit for a couple of minutes Wipe dry with a clean soft cloth Do not saturate the sensor or immerse the sensor in a cleaner Power the unit off open the chamber and remove the IR plate Pull down on the dummy unscrew the cable from the top of the dummy and press the dummy into the clip on the plate See Figure 5 12 Screw the cable onto the top of the IR sensor The cable is keyed and can only be inserted one way Reinstall the sensor by pushing it as far as it will go into the hole After the unit is powered back on with the IR sensor reinstalled the display toggles between CYC COMPLETE REPL HEPAs and PRESS ENTER AUN SET CAL CON HEAT 30 0
29. 0 eae d POWER SUPPLY T A 400119 400132 FOR 134 ie KIT 190779 L3 Lf X gas 4 eo Be 77 pe4 LS 22 hL 2 DISPLAY BRO 190753 b RIBBON CABLE J4 1 5 196018 25 27 L J4 3 o J4 4 28 4 5 J4 5 30 3l 2 8 Re 4 8 33 4 COM JA 10 M 2 OPEN Jil 35 3 CLOSED poe 36 5VOC POWER INLET RFI LINE FILTER 460180 POWER SWITCH CIRCUIT BREAKER 12 230180 EXTERIOR CONVIENCE OUTLET 460138 320 TRANSFORMER IKVA 420104 INTERIOR FAN MOTOR 115VAC 50 60Hz 156117 COMPONENT COOLING FAN 900135 amp CONTROL T STAT COR 400151 SAFETY MANUAL RESET Rise 400150 CHAMBER HEATERS BACK 39 156W 132058 BOTTOM 28 1120 132059 LEFT 23 92W 132060 RIGHT 23 92W 132061 COLLAR HEATER 48 1921 132062 DOOR HEATER 89 356W 132057 STERILIZATION PROCESS INDICATION 02 SOLENOID 12 0 250085 DOOR SWITCH INNER 360239 Shown w door open STERILIZATION INITIATE BUTTON 360236 DOOR SWITCH OUTER Shown w door open 360230 TEMP PROBE 2252 OHMS 2250 290184 MODEL 371 SENSOR 290168 Direct Heat Incubator with Sterilization Electrical Schenatic 1 of 3 371 70 0 0 REV 4 Thermo Electron Corporation Steri Cycle COz Incubator 9 4 Section 9 Electrical Schematics 39
30. 12 EN 1 5VOC Y 370 70 0 0 REV 5 of 3 Thermo Fisher Scientific Direct Heat C02 Incubator 9 1 Section 9 Electrical Schematics 9 2 O oon Ul w oh Gh xm I OO CD Ul 75 76 lt 6 lt 5 N 37 4 i E ES a RH 39 2 4l 45 5 REM lt 4 395 485 Ha 1 55 45 1 fae sel 42 JI 2 r h J1 3 He 4 48 J1 5 ELM 31 6 3 con 1 7 4 TEMP ANALOG 02 n aid OUTPUT BOARD 5 2 RH Y 9 02 9 J13 1 220 N C J13 2 47 48 0 213 3 eo o 13 4 48 50 4113 5 e J13 6 51 1 47 2 J13 7 Ga del J7 5 J7 6 0 Q4 Ho 2 REMOTE ALARM CONNECTIONS shown in alarn state OPTIONAL RS 485 OUTPUT KIT 1900085 OPTIONAL RH SENSOR KIT 1900091 3598 3599 3584 3585 OPTIONAL ANALOG OUTPUT KITS 190512 4 to 20 190543 0 to 5700 190544 0 to 1VDC OPTIONAL GAS GUARD KIT 1900086 C02 PRESSURE SWITCH 1 TANK EMPTY 360213 PRESSURE SWITCH 2 TANK EMPTY 360213 SOLENOID 3 WAY 12 0 250121 MODEL 380 IR 02 SYSTEM 231204 Electrical Schenatic Made Direct Heat Incubators with Sterilization Cycle 370 70 0 0
31. 2 Press the right arrow until TMP RLY displays 3 Press the up down key to toggle the setting ON OFE 4 Press ENTER to save the setting 5 Press the MODE key to return to RUN mode or the right left arrow to go to next previous parameter Direct Heat C02 Incubator 3 3 Section 3 Configuration Set Low CO Alarm The low alarm limit is the deviation from the setpoint which imi will cause low CO alarm The setpoint is variable from 0 5 CO Limit tracking alarm below setpoint to 5 0 below setpoint The factory setting is 1 0 below setpoint A minus sign in the display indicates that the alarm setting is below the setpoint 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until CO2 LO X X shows in the message display 3 Press up down arrow to change the low alarm limit 4 Press ENTER to save the low alarm limit 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter Set High Alarm The high alarm limit is the deviation from the setpoint which Sor Limit tracking alarm 5 0 CO above setpoint The factory 108 above setpoint 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until CO2 HI X X shows in the message display 3 Press up down arrow to change the high CO alarm limit 4 Press ENTER to save the high alar
32. 4 The fitting is labeled CO2 Inlet 1 Tank Make sure that the connections are secured with clamps Check all fittings for leaks With the incubator properly installed and connected to power the humidity pan filled and the unit connected to gas supplies system setpoints can be entered The following setpoints can be entered in SET mode Temperature Overtemperature and CO To enter SET mode press the MODE key until the SET indicator lights Press the right and or left arrow keys until the proper parameter appears in the message display See Chart 1 1 for more detail Series 8000DH incubators have an operating temperature range of 10 C to 50 C depending on ambient temperature The incubator is shipped from the factory with a temperature setpoint of 10 C At this setting all heaters are turned off Thermo Fisher Scientific Section 1 Installation and Start Up Setting the Operating To change the operating temperature setpoint Temperature continued Press the MODE key until the SET indicator lights 2 Press the right arrow until Temp is displayed in the message display 3 Press the up down arrow key until the desired temperature setpoint is displayed 4 Press ENTER to save the setpoint 5 Press the MODE key until the RUN indicator lights for RUN mode or press the right left arrow keys to go to next previous parameter Setting the Overtemp Caution The independent overtemp system is designed as
33. 79 90 0 90 VN 51 38 30 114190530 0 2 N23 1v6E OL NOWWOD 1 8 00 865 8 8 7 Direct Heat CO2 Incubator ientific Thermo Fisher Sc Tri 8 8 002 01 lt Tay 2066 1 5 OVSE BESE OLNONNOD I 371 031312345 SS31NQ 32NYU3101 011 304305 NONI2373 005 0 31V2S 1071218 31V0 01 0487 820 QvO 37 0 IONGHRICIH NOOH 38100555 I YM 1nOHLIM 3504404 303 4350 5405835 202 31111 9MQ YON 1504804 801 83 10 Ol 035012510 38 OL LON SI NOILYNYOINI HONS ONY NOIIVNUOJNI MOLVENONT 531835 OLE 3VN LYVd TIGON Auvi3ludOHd SNIVINOD LNAWNDOd SIHL 183559 YOSNAS 21 LNVIVIS ALY 560614 ISN 1943557 405535 401 18 35 50518 ALY ISN ONIY 9314 911 18 66 006 1 01 OLE 4015 1 0760061 6 401 3811 031010AN02 211772 8 NNOW YOSNAS Y 502152 1 35 ALY 560614350 MOSN3S 02152 9 139519 72020 5 ONIY 8314 0 2111 16 9260061
34. 90392 are available through your local distributor The remote alarm provides a NO normally open output an NC normally closed output and COM common Refer to Figure 6 1 The contacts will trip on a power outage or an overtemperature condition The contacts may also be programmed to trip or not trip on temperature alarms alarms and RH alarms See Section 3 Configuration mode NO NC green wire red wire SV max at VA COM not connected yellow wire black wire AMH Alarm Contacts Junction Box IMPORTANT USER INFORMATION Caution Stored product should be phone cable to incubator alarm contacts RJ 11 connector protected by an activated alarm system ry sedes of initiating a timely response 24 hours day These alarms provide interconnect for centralized monitoring All outputs shown in alarm condition Figure 6 1 Remote Alarm Contacts CAUTION Contains Parts and Assemblies Susceptible to Damage by Electrostatic Discharge ESD Direct Heat CO2 Incubator 6 1 Section 6 Factory Options 6 2 Humidity Readout Direct Heat CO2 Incubator Series 8000DH incubators are equipped with a humidity sensor to monitor the relative humidity inside the chamber The sensor is located on the chamber ceiling and provides a signal that is displayed in 1 increments on the control panel The humidity readout can display continuously or toggle with the temperature r
35. A fm 02 is higher than CO2 High Tracking Alarm 02 is lower than CO2 Low Tracking Alarm TEMP is lower TEMP Low Tracking Alarm RH is lower than RH Low Limit Alarm RH option All alarm delays and ringback times are 30 seconds Temperature Controller Failure See Sect 4 TMP CTRL ERR Omin 15min es No ES No No No When multiple alarm conditions occur active messages are shown in the message display one at a time updating at 5 second intervals Pressing 25 during multiple alarms causes all active alarms to be silenced and to ring back in 15 minutes The TEMP IS LOW alarm is disabled when the Temp set point is 10 C The alarms are disabled when the set point is 0 0 Thermo Fisher Scientific Direct Heat C02 Incubator 4 1 Section 4 Alarms Temp Controller Failure 4 2 TMP CNTR ERR Sensor Fault Alarms Direct Heat CO2 Incubator In addition to other safety features designed into Series 8000DH incubators a thermostat is provided to monitor the cabinet s temperature In the unlikely event of a temperature control failure the thermostat will turn off all heaters at a cabinet temperature of 160 C 5 This is intended to be a safety feature to protect the incubator and is not intended to protect the cell cultures or the equipment inside the chamber should a temperature control failure occur Should such a failure occur contact the Technical Services Departmen
36. A el 52 POWER INLET RFI LINE FILTER 3 460180 2 POWER SWITCH CIRCUIT BREAKER 12 3 2 230180 1 ota EXTERIOR CONVIENCE OUTLET 4 FUSE 5 460024 230135 5 115 TRANSFORMER IKVA 230 0 420104 7 INTERIOR FAN MOTOR 115 SO 60Hz 156117 8 COMPONENT COOLING 900135 amp CONTROL T STAT COR 400151 9 4 OVERTEMP SAFETY MANUAL RESET Open on Rise 400150 0 CHAMBER HEATERS BACK 39 156W 132058 BOTTOM 28 1 12W 132059 LEFT 23 92 32060 RIGHT 23 928 132061 COLLAR HEATER 48 1824 H 2 132062 3 145 2 554 10 D 0008 HEATER 89 3560 L__ J 132057 4 5 5 5 3 1 2 6 7 ca 9 LN 02 33 POWER SUPPLY 20 EON m 8 STERILIZATIDN PROCESS INDICATION KIT 190779 e on 21 CZ 3 5 19 4 CO2 SOLENDID 1206 e amp gt OG 250085 WEG 22 gt __2l _h ig 23 2 1 8 2 24 L__24 DISPLAY 880 25 190793 26 A 27 RIBBON CABLE 25 DOOR SWITCH INNER 360239 e 196018 Shown w door open 28 44 2 25 29 44 3 9 27 ps STERILIZATIUN INITIATE BUTTUN e E 360238 30 4 4 n 3l 5 E DOOR SWITCH OUTER 1 Shown w door open 360230 32 4 6 W 33 TEMP PROBE 2252 DHM 2250 d 290184 34 J4 8 32 3 Electrical Schematic 35 4 9 3 4 COM Model n 36 4 10 Direct Heat MODEL 370 37 J4 11 M 3 3 CLOSED 02 SENSOR Incubators with 280188 CP ALI Sterilization Cycle 38 J4
37. I G1 2160061 8 83010045 85004 L 3425 5835 1x3 SS H I 07 9 250065 9 3245 1906 X vid v I 99096 1 091 I1NISOMBHI 161000 JHOLINS 611007 NZX YI ASNA 6100 2 asna 010 22 X 0018 3801 60102 0 Isna 887022 6 asna 65502 8 08218 YOLVENINI 91835 ove 0596 L 2 NOTAN 970091 9 2 14096 38IM NOTAN 16011 9 348 SS 8 6 X 07 1006 INN 42 Ov r _ 90062 INN 42 z 84 2002 2 2 MIYOS SS X 26 99 51022 ALO 2530 LBV SIVIYSLYW 30 1118 531935 0008 OL 0300Y 979 10 22 10 BSLE NI 6 SINN 4112 550019 Lo v0 10 SLE NI 1 310N 0300V 9 9 190 08 90 90 NI 5830945 980916 v 0300Y 909 979120 52 60 9 Z 2 0065 Ol 62062 06122 Wow 10 2 01 7662 01 1 NOILONGOYd 804 03573738 820 820 10 82 lt VIN 0 NOISIA3N 30 0114182530 dav 075 AG 31 4 ON 3 A38 756 27567 156075679606 01 NOWNOD 8 9 002 01 YIEGWAN OMG lentitic Thermo Fisher Sc Direct Heat 02 Incubator 8 6 Section 8 Spare Parts 53 30 3ALLISN3S 5y
38. O2 Incubator 1 1 Section 1 Installation and Start Up 1 2 05 Mutes the audible alarm Alarm Indicator Light pulses on off during an alarm condition MODE Select Switch Used to select Run Setpoints Calibration and Configuration modes Message Display Shows system status Mode Select Indicators RUN Run Menu SET Set Points Menu CAL Calibration Menu CON Configuration Menu HEAT Indicator Lights when heaters are activated INJ Indicator Lights during injection into chamber T C Display Programmable to display temperature See Configuration section RH Display Shows percentage of humidity See Configuration sec tion Display Programmable to display percentage See Configuration section Arrow Keys Scroll for Parameters Increases or decreases number values toggles between mode choices ENTER Stores the selected value into unit memory Silence Power Switch Alarm Mode Selector Switch Indicator Switch ENTER Mode Select Indicators in C button youn M RUN SET CRL CON ja INJ CO 71 Dey Message Display A 0 t 7 SCROLL F R PARAMETERS Active Injection Indicator Scroll Arrow Keys Humidity in 96 Figure 1 2 Control Panel Direct Heat 02 Incubator level in Thermo Fisher Scientific Section 1 Installation
39. RI CYCLE I R CO2 INCUBATOR 3584 STERI CYCLE T C CO2 INCUBATOR RH DISPLAY 3585 STERI CYCLE I R CO2 INCUBATOR RH DISPLAY 3540 STERI CYCLE T C CO2 INCUBATOR 115 VOLT 3542 STERI CYCLE I R CO2 INCUBATOR 115 VOLT 5 3759 0 GJG GJG CCS ADDED MODEL NUMBERS 4 IN 3306 GJG GJG MSB ADDED MODEL NUMBERS 3 3124 KOG LON CHANGED 290137 TEMP PROBE Electr Schenatic Tare OD CATA Mode oA Direct Heot a p P WHEAT p HOT ee aer Incubotors with ATTENTION on Gy Sy oro OF 2427 11204100 Cycle OBSERVE PRECALITIONS MATERIAL ELECTROSTATIC PAINT me 370 70 0 0 REV 5 SENSITIVE DEVICES Thermo Co oe SIZE Page 3 98 3 Thermo Fisher Scientific Direct Heat C02 Incubator 9 3 Section 9 Electrical Schematics On CD Ul On Ul d UJ 175 ctu ees NO NO 0 1 CD amp WN CO CO C WY POWER CONNECTION 230VAC 50 60 7 1PH 4 4 FLA 2 e 3 5 ml 230 0 tarred E i 9 t ores 15 1 g i 2 ee 5 5 4 10 0 4 45 6 2 Pl i T Eh 0
40. Series 8000DH 0 Incubator Direct Heat with Sterilization Cycle Operating and Maintenance Manual 7003540 Rev 7 Thermo SCIENTIFIC Preface Model Sensor Voltage 3540 T C 115 3541 T C 230 3542 IR 115 3543 IR 230 is a thermal conductivity sensor IR is an infra red sensor All units are 50 60 Hz MANUAL NUMBER 7003540 1 25584 8 6 09 Updated RH low alarm ringback time from 30 minutes to 15 CCS 6 25248 1 3945 6 9 09 Updated drawer specs sliding and stationary CCS 5 24900 IN 3923 4 22 09 Updated door interlock switch 3598 200 9 CCS 4 25303 IN 3902 2 17 09 UL decom warning pg 1 6 CCS 3 24975 12 10 08 Updated water specs on pages 1 10 and 1 11 CCS 2 24344 IN 3824 1 16 08 Removed stud from back panel chg d grnd wire placement updated CAD drawings ccs 1 24173 6 21 07 Updated with Thermo data CCS 0 1 25 07 Original manual CCS REV ECR ECN DATE DESCRIPTION By Thermo Fisher Scientific Direct Heat C02 Incubator i Preface CAUTION Contains Parts and Assemblies Susceptible to Damage by Electrostatic Discharge ESD Important Read this instruction manual Failure to read understand and follow the instructions in this manual may result in damage to the unit injury to operating personnel and poor equipment performance Caution All internal adjustments and maintenance must be performed by qualified service personnel Material in this manual is for information
41. THIS DOCUMENT CONTAINS PROPRIETARY MODEL PaRT NAYE 371 8 381 HEAT STERILIZATION INCUBATOR Incubator with ATTENT I ON DISCLOSED TO OTHERS FOR ANY PURPOSE NOR DWG TITLE ELECTRICAL SCHEMATIC Ster zot aa o Gy cy an DNF 8 12 00 SCALE NONE OBSERVE PRECAUTIONS MATERIAL ELECTROSTATIC Thermo OTHERWISE SPECIFIED DRAWING NUMBER SIZE 4 SENSITIVE DEVICES ELECTRON ANGLES DECIMAL 371 70 0 0 Steri Cycle 02 Incubator 9 6 60 7 V L006 OSI JO 40jnqujsip e20 Jno WSN 34 episjino jeloeds pue uo suonsenb noA 3148M 9 t 0t4 L Jo pue YSN 587 8 7 008 1 1e seorues jeo se jd 4 eoueuojuleuJ pue uonejejsur pejulud jueuudinbe jno si sejes e20 INOA SjonpoJd 5501 Jo 1501 0 jenuenb
42. Thermo Fisher Scientific Direct Heat C02 Incubator 1 9 Section 1 Installation and Start Up 1 10 Electrical Power Connection cont Filling the Humidity Direct Heat C02 Incubator Pan Plug the provided power cord into the power inlet connector on the back of the cabinet Figure 1 14 then into the grounded dedicated electrical circuit Accessory Outlet 75 watts max ie A ka A S Remote TUM Air Power Cord Alarm Contacts d intake Connector 30V max 1A max RJ Il telephone style connectors IR CO units only Figure 1 14 Back of Cabinet Fill the humidity pan with sterile distilled water to within 1 2 of the top Place the pan directly in the center of the incubator floor For applications requiring high humidity the pan should be placed against the left side wall of the incubator The side ducts have been modified to allow the pan to be placed against the wall Optimum humidity is achieved by capping the CO sample port This will however cause condensation in the chamber To enhance RH recovery from door openings place a second humidity pan in the right side duct For best operation of the incubator sterilized distilled demineralized or de ionized water should be used in the humidity pan Water purity should be in the resistance range of 50K to 1M Ohm
43. alarm be programmed to trip the remote alarm contacts Trip Co ntacts A setting of ON allows this a setting of OFF blocks the RH alarm from tripping the contacts The factory setting is ON 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until RH RLY XXX shows in the message display 3 Press up down arrow to toggle the setting ON OFF 4 Press ENTER to save the setting 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter Enabling Temp RH to os uni equipped with the RH option the upper seven segment display H on the control panel can be configured to display Temp continuously RH be Displayed continuously or toggle between Temp and RH If the units does not have RH the upper display always shows temperature If temperature is set to ON and the RH is set OFF temperature shows continuously If temperature is set to OFF and RH is set to ON RH shows continuously If both are turned ON the display toggles between the two The factory setting defaults to toggle mode if the RH option is present 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until DISP TMP XXX or DISP RH XXX shows in the message display 3 Press up down arrow to toggle the setting ON OFF 4 Press ENTER to save the setting 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter Thermo Fisher Scientific Direct H
44. and Start Up Keyp ad Ope ration The Series 8000DH incubator has four basic modes for incubator setup Run Setpoints Calibration and Configuration Run is the default mode for normal operation e Set is used to enter system setpoints for incubator operation e Calibration is used to calibrate various system parameters e Configuration allows for custom setup of various options Table 1 1 Selections Under Each Mode m IS Dnm RUN SETPOINT CALIBRATION CONFIGURATION Default Mode Temperature Temp Offset Audible Overtemp C0 Cal New HEPA Timer C0 IR Cal Replace HEPA Reminder RH Cal Access Code Temp Lo Alarm Temp Relay 0 Lo Alarm Hi Alarm C0 Relay C02Z amp S s RH Lo Alarm Relay Display Temp Display RH units Base Unit Displays 2 units Option Unit Displays units only Thermo Fisher Scientific Direct Heat CO2 Incubator 1 3 Section 1 Installation and Start Up Keypad Operation continued Displays Installing the Incubator 1 4 Direct Heat CO2 Incubator SCROLL FOR PARAMETERS Arrows Steps the operator through parameters of SET CAL and CON modes The right arrow goes to the next parameter the left arrow returns to the previous parameter Up Down Arrows Increases decreases or toggles the parameter value selected in SET CAL and CON modes ENTER Must press this key to save to memory all changed values Key Press to mute audible alarm See Section 4 f
45. bator atmosphere In monitoring the effects of air temperature and absolute humidity must be held constant so any change in thermal conductivity is caused by a change in CO concentration Changing temperature or changing from elevated humidity to room ambient humidity levels will necessitate re calibration of the control Some T C CO sensors go through an aging period especially on new installations Calibration should be checked on a weekly basis and adjusted as necessary When stabilization occurs checks can become less frequent T C CO Sensor Stabilization Periods Start Up The CO sensor has been calibrated at the factory for 37 C and elevated humidity Allow the temperature humidity and levels in the chamber to stabilize at least 12 hours before checking the concentration with an independent instrument Presently Operating Make sure the chamber doors are closed Allow at least 2 hours after the temperature and displays reach their setpoints for chamber atmosphere stabilization 2 2 Direct Heat CO2 Incubator Thermo Fisher Scientific Thermal Conductivity CO System continued Infrared System Thermo Fisher Scientific Section 2 Calibration Make sure the stabilization periods outlined above are followed Sample the chamber atmosphere through the sample port with an independent instrument Sample the atmosphere at least 3 times to ensure accuracy of the instr
46. bilization periods outlined above 2 With a full humidity pan and stable temperature the relative humidity in the chamber will be 95 3 Using Step 3 5 of the relative humidity sensor adjustment adjust the display to 95 This calibration method should be accurate to within 5 Thermo Fisher Scientific Direct Heat C02 Incubator 2 5 Section 2 Calibration Chart 2 1 Calibration Mode Press MODE to light CAL gt v w t9 9 B To Calibrate Press MODE to move Numbers increase to SYS CONFIG mode Press Enter to T P 9 TEMPCAL XXX gt save the setting emperature N Press lt to return T Numbers decrease to previous parameter A Numbers increase T C 0 Press Enter to CO2CAL XXX ENTER when Thermal Conductivity save the setting USO Press to return EM CUM Numbers decrease to previous parameter LUE A Numbers increase IR Press Enter to IR CAL when IR sensor is in use save the setting Press lt to return Numbers decrease to previous parameter SCROLL FOR PARAMETERS A Numbers increase Percent RH RH XX Press Enter to when RH option is in use Press lt J to return to p
47. e 1 7 6 Align the slotted holes in the brackets with the mounting holes on the back of the top incubator Secure the brackets with the screws and washers provided in the parts bag See Figure 1 8 Top Incubator back e 000000000000000000000000 Stacking Stacking T bracket bracket SHEER Ott Bottom Incubator back wm ce It 1 7 Bracket Figure 1 8 Installed Brackets on Back of Unit into Slot 7 Thread 1 4 x 20 bolt washer included with the stacking brackets into the hole behind the cover plate Do not tighten Refer to Figure 1 9 Cover plate and bolt hole with door connector 8 Remove the cover plate from the same area on the other side of the top unit Figure 1 9 Bolt Hole 9 Thread the other 1 4 x 20 bolt and washer into this hole 10 Tighten the bolts on both sides 11 Assemble the door hinges to the unit Secure with the screws 12 Plug the door cord into the connector as previously Secure the cover plate 13 Install the cover plate on the other side of the unit The stacked incubators are ready for service 1 Remove vinyl from shelf channels duct sheets and air duct if present 2 Using a suitable laboratory disinfectant clean all interior surfaces Caution Before using any cleaning or decontamination method except those recommended by the manufacturer users should check with the manufacturer tha
48. eadout In addition a low alarm limit can be set on the humidity readout to detect when the humidity pan runs dry Refer to Section 3 Configuration The sensor is capable of measuring relative humidity from 10 to 100 with an accuracy of 5 above 90 See Section 2 Calibration for details on calibrating the humidity readout Factors which may affect humidity levels in the chamber are the water level in the humidity pan frequency of door openings and the fact that the humidity pan is located on the bottom of the chamber versus on a shelf Caution Incubators equipped with a T C thermal conductivity sensor rely on a constant level of relative humidity to accurately measure and control the concentration in the incubator Thermo Fisher Scientific Thermo Fisher Scientific Section7 Specifications to 25 C Temperature Control 0 1 C Microprocessor PID Control Setpoint Digital Touch pad 0 1 C 5 above ambient to 50 Uniformity 0 3 37 C Tracking alarm User programmable low indicator Overtemp Tracking user programmable action and indicator Display Digital LED 0 1 C increments Temperature Safety Extreme temperature safety action and indicator Sensor Thermostat independent of temp control system Indicator Message center audible and visual alarms Relative Humidity Control
49. eat C02 Incubator 3 7 Section 3 Configuration Chart 3 1 Configuration Mode Page 1 of 3 Press MODE to light CON To Configure Press MODE to return A Audible alarm ON to RUN mode AUDIBLE Press Enter to AUDIBLE Audible Alarm save the setting Press lt 1 to return to previous parameter Audible alarm OFF NEW HEPA Filter Press Enter to Dm restart the timer and clear the REPLACE HEPA alarm Press lt 1 to retum to previous parameter Numbers increase Press Enter to save the chosen of months REPLACE HEPA Timer Press lt to return to previous parameter Numbers decrease Numbers increase Press Enter t 960 Press lt 1 to return to previous parameter FOR FARANE TERS Access Code Numbers decrease Numbers increase Press Enter to save the setting Press lt 1 toreturn to previous parameter SCROLL FOR PARAE TERS Low Temp Alarm Limit Numbers decrease Temp relay ON Low Temp Alarm Relay Press to return to previous parameter Temp relay OFF continue on next page 3 8 Direct Heat CO2 Incubator Thermo Fisher Scientific Section 3 Configuration Chart 3 1 Configuration Mode Page 2 of 3 refer to previous page To Configure Numbers increase Press Enter to 00210 XX n save the setting Press toret
50. ermo Fisher Scientific Section 1 Installation and Start Up HEPA Fi Iter Caution Be careful when the filter The media can be damaged if it is Installation mishandled To avoid damage to the incubator do not operate the unit without the HEPA filter in place 1 Remove the filter from the shipping box 2 Remove the plastic coating from the filter using caution not to touch the filter media 3 Install the filter as shown in Figure 1 13 Refer to Section 5 for HEPA filter maintenance Caution The media of the filter can be damaged if mishandled To avoid damage to the incubator do not operate the unit without the HEPA filter in place Leveling the Unit Check the unit for being level by placing a bubble style level on one of the shelves Turn the hex nut on the leveler counterclockwise to lengthen the leg or clockwise to shorten it Level the unit front to back and left to right Electrical Power See the serial tag on the side of the unit for electrical specifications or refer Connection to the electrical schematics at the end of this manual Caution Serial tag rating is based on amperage draw during sterilization cycle Normal operating amperage is much less Ensure that electrical circuit will handle amp draw of sterilization cycle Connect the incubator to a grounded dedicated circuit The power cord connector is the mains disconnect device for the incubator Position the unit so that it can be easily disconnected
51. espe iradis gas regulator 21662425 kiwi asas d duds Gas shuttle switch DDD NE External gas guard 9509316 Wall clamp for bottle 231203 RH sensor assembly P N 1900091 RH display option 230180 12A DPDT circuit breaker switch power Ls Switcher 40W 12 5 12V 196018 34 position control to display ribbon cable Inner door switch 360230 a dee cand aetna oe br Outer door switch 2507368222 RES Sterilization initiation switch Blower wheel 19098525 Access port filter assembly 760210 Disposable in line filters 10 filters 770001 Disposable filter 99 97 2 line air sample 7600175 i filter 5 5 diameter 750209 dons value pack 4 filters 1900067 osser areires HEPA filter replacement kit 190650 Independent inner door kit 190600 ees ea ea Right hand door swing 1800063 amp sua nso e vx er ERR Rees Roller base 140387 xus qa d Va e Overlay 30109579 ee odes Aqua water cleaning cell 4 pack E for water reservoirs pans and bottles 1900346 _ Display control board Thermo Fisher Scientific Direct Heat CO2 Incubator 8 1 Section 8 Spare Parts 2 002 01 lt Em 319NV TWWI230 9NIMVEG 031312345 SSIINN 32NY83101
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53. hannel forward and engage the channel s front slot into the side duct s appropriate forward tab Refer to Figure 1 11 4 Figure 1 10 shows one of the channels installed on the right side duct Direct Heat CO2 Incubator 1 7 Section 1 Installation and Start Up Access Port Filter amp 1 Locate the opening in the top left corner of the interior chamber Sensor Cover Plate Remove the tape from the opening on the outside of the unit 2 Locate the stopper with filter in the hardware bag Lift the metal port cover and install the assembly in the opening inside the chamber See Figure 1 12 3 Also in the hardware bag is sensor cover plate Install using the 1 4 turn fasteners Refer to Figure 1 12 for the location Thumbscrews gt CO Sensor Mss Filter Cover Plate ccess Port Filter Air Sample and stopper Filter assembly J Figure 1 12 Filter and Plate Air Sample Filter 1 Remove the filter from the shipping bag Installation 2 Install the air sample filter assembly to the black hose barb behind the top duct 3 Insert the other end of the filter assembly onto the metal tubing on the top duct Refer to the Figure 1 13 behind top duct Air Sample Filter Figure 1 13 Air Sample Filter Installation 1 8 Direct Heat CO2 Incubator Th
54. he air sample filter tubing shelves and ducts with disinfectant then rinse with sterile water Optionally the shelves and ducts may be autoclaved Wash the inner door gasket with disinfectant This gasket may be removed to be cleaned or replaced Wash the cabinet interior with disinfectant starting at the top and working down Refer to the disinfectant directions for length of time needed before rinsing Wash the inner door both inside and out The cabinet and door must be rinsed with sterile water until the disinfectant has been removed After the cabinet has been rinsed spray with 70 alcohol Install the left and right ducts inner door gasket access port filter assembly and air sample filter and tubing spraying each with 70 alcohol Install a new HEPA filter Install the shelves and spray with 70 alcohol ean the incubator exterior with a damp sponge or soft well wrung clot Clean Cabinet Clean the incub damp sponge or soft well wrung cloth Exterior and mild detergent dissolved in water Dry with a soft cloth 5 2 Direct Heat CO2 Incubator Thermo Fisher Scientific Section 5 Routine Maintenance Clean Glass Doors The chamber glass door and the optional independent inner glass doors may be cleaned using the same disinfectant as used on the incubator interior It is imperative that they be rinsed with sterile distilled water to remove the disinfectant residue The doors should then be dried with a sof
55. ing n transformer CO lenoid COz microbiological 3 A filter Component fan Filtered line cord Ee Foon nector Remote 7 7 Back of incubator alarm contacts Accessory connector and outlet bW max wire through put Figure 5 3 Electronics Drawer Direct Heat CO2 Incubator 5 5 Section 5 Routine Maintenance Sterilization Cycle Information You Need to Know Before Starting a Cycle The Sterilization Cycle requires approximately 12 hours heat up 2 4 hours sterilize 2 hours and cool down 6 8 hours Additional time is needed to verify the calibration of temperature and after the cycle is complete e During the Sterilization Cycle the incubator updates the temperature to the analog output board and the 1535 however will be fixed at setpoint and RH will be fixed at RH Low Limit plus 1 e A HEPA filter replacement kit see the spare parts list should be on hand prior to initiating the Sterilization Cycle Information About the Cycle e Pre cleaning may be required In order to avoid odors stains on the interior baked on material etc wipe off all visible signs of spills 4 Odor may occur during the Sterilization Cycle and is considered normal The Steri Cycle is not intended to sterilize other items instruments etc from the lab e During the cycle the unit chamber becomes hot enough to melt samp
56. key until the RUN indicator lights or press the right or left arrow to go to the next or previous parameter All T C cells are calibrated at the factory at 37 C high humidity and 10 Therefore if a temperature setpoint of 37 C has been entered the humidity pan has been filled and the control is to run between 0 10 with a T C sensor setpoint may be entered immediately Otherwise it is important to allow the unit 12 hours to stabilize at the temperature setpoint before entering the setpoint Both models of the incubator have a setpoint range of 0 0 to 20 0 The incubator is shipped from the factory with a setpoint of 0 0 At this setting all control and alarms are turned off To change the setpoint 1 Press the MODE key until the SET indicator lights 2 Press the right arrow until CO2 XX X is displayed in the message display 3 Press the up down arrows until the desired setpoint is displayed 4 Press ENTER to save the setpoint 5 Press the MODE key until the RUN indicator lights to go to RUN mode or press the right left arrow keys to go to next previous parameter Thermo Fisher Scientific Section 1 Installation and Start Up Chart 1 1 Set Mode Press MODE to light SET S wong ee d 799 To Set Press MODE to move N
57. lay If no keys are pressed for approximately five minutes while in Calibration mode the system resets to RUN mode so control functions are reactivated Caution Before making any calibration or adjustments to the unit it is imperative that all reference instruments be properly calibrated A Tempe rature Before calibration allow the cabinet temperature to stabilize Place the calibrated instrument in the center of the chamber The instrument should be in the airflow not against the shelf Temperature Stabilization Periods Startup Allow 12 hours for the temperature in the cabinet to stabilize before proceeding Already Operating Allow at least 2 hours after the display reaches setpoint for temperature to stabilize before proceeding Thermo Fisher Scientific Direct Heat C02 Incubator 2 1 Section 2 Calibration Temperature 1 Press the MODE key until CAL indicator lights continued 2 Press the right arrow until TEMPCAL XX X appears in the message display 3 Press up down arrow to match display to calibrated instrument 4 Press ENTER to store calibration 5 Press the MODE key to return to RUN or the right left arrow to go to next previous parameter Thermal Conductivity Models 3540 and 3541 have a thermal conductivity T C sensor CO System Thermal conductivity of the incubator atmosphere is not only effected by the quantity of CO present but also by the air temperature and water vapor present in the incu
58. les instruments dishes etc left inside the unit The items listed below also need to be removed 1 HEPA filter 2 Air sample filter 3 Access port filter assembly 4 Water in the humidity pan 5 Temp RH recorder probe if applicable 6 IR sensor if applicable v PP ZN Air Sample Port Filter Filter e During the Sterilization Cycle discoloration stopper and tubing assembly 4 of some materials may occur For example stainless steel turns a straw color after a period of exposure to high temperatures This is normal 5 6 Direct Heat C02 Incubator Thermo Fisher Scientific Section 5 Routine Maintenance Checkpoints What if Then Cycle does not initiate or terminates in mid cycle Check for alarms SYS IN AIR SNSR ERR TMP CTRL ERR Units are stacked A Sterilization Cycle performed on either unit affects performance on second unit Do not use second unit during Sterilization Cycle The Sterilization Cycle may be performed on both units simultaneously with proper electrical connection No action is taken within 1 minute following display prompt Unit returns to normal operation SYSTEM OK Need to cancel cycle in progress Hold down green cycle initiation button for 3 seconds Water is not removed from humidity pan during cycle Steam is produced and may cause burns Listed components are not removed before initiating cycle Components cannot withstand the sterilization cycle temperatures a
59. ltage protection means For example in CAT which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V supply 2 Pollution Degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only non conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation Thermo Fisher Scientific Direct Heat C02 Incubator 7 3 Section 8 Spare Parts Part Description 190699 ERR Removable feather gasket 190670 d serere Duct sheet and shelf channel kit Stainless steel shelf 190884 Stainless steel shelf and channels 191650 Micro board main control 230135 Fuse 1A TD 5 x 20mm accessory outlet 115V 230120 Fuse 0 5A TD 5 x 20mm accessory outlet 230V er eat Cordset 10A 250V 156117 so ewe ws Motor 2 pole 115VAC 50 60 Hz Display board 290184 Probe 2252 Ohm 25 C 1 8 x2 130077 52 50 RUE Stopper with 3 8 hole 290168 Thermal conductivity T C type sensor Infrared type sensor 250087 valve kit with assorted fittings 963010 tie
60. m limit 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter 3 4 Direct Heat CO2 Incubator Thermo Fisher Scientific Section 3 Configuration Enable CO Alarms to High and Low alarms can be programmed to trip the remote alarm Trip Co ntacts A setting of ON allows this a setting of OFF blocks alarms from tripping the contacts The factory setting is ON 1 2 Press the MODE key until the CON indicator lights Press the right arrow until CO2 RLY XXX shows in the message display Press up down arrow to toggle the setting ON OFF Press ENTER to save the setting Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter Set New Zero Number new T C sensor is being installed the two numbers on the for T C Sensors factory installed sticker on the T C cell must be entered to calibrate in the unit Note For the technician s convenience a label containing the two numbers on the T C cell is affixed inside the electronics drawer A 1 Thermo Fisher Scientific Press the MODE key until the CON indicator lights Press the right arrow until T CZ XXXX shows in the message display Press up down arrow to change the zero number to match the sticker Press ENTER to save the setting Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter Direct Heat CO2 I
61. n 8 Spare Parts 2102 Z AIS 0 0 5 00 888 119130 HILINS 2000 JINNI 05 0 372 SONA 000 JINNI 7119130 05 70 TWJS 71130 130191 1 STONVH 000 anraalx3 0620061 97 1 000 724 JAWS PLE TANS SY 1 0 0 1237 2022 3801 LIMNI vy 1 6102 TANIA 38 LIANI 2021 8 10 1 ASSY 31V ld 353 1 10 00050 2 1 ATAWASSY 0 LNUSS 1 1 845 1 ATBN3SSV 1 10 00010 6 Ov 1 ATEWASSY TANVd ADVE O E 5 002 0 1 MOSNAS OLE BE LE 1 AIJEW3SSY 9000 AINNI 1 S1 EEE0061 1 ATBN3SSV 8000 01891 4 1 20 2012866 SE 1 ATBW3SSV B3AUJ 301 1 91 802006 1 amp 1 amp 1 1 1 0 0061 EE 1 INA 34979 SONA 4000 55719 JINNI 1 91 281006 ZE 1 0 071 9 4141 7331 3817 Ladd 9 51108 1 1 3NDOITIS 000 YANNI OLE 801 01 OE 1 13450 OLE 201601 82 1 3141 HILIMS AG JINNI 7 _ 82 1 Wal 1053 _ Le 1 MIGWASSY 5000 JINNI 92 1 3710034 X 1 920078 1 7 vId3H 3181509510
62. nance or with technical or special application inquiries contact your local distributor Repairs NOT Covered Under Warranty Calibration of control parameters Nominal calibrations are performed at the factory typically 1 C for temperature 1 for gases and 5 for humidity Our service personnel can provide precise calibrations as a billable service at your location Calibration after a warranty repair is covered under the warranty Damage resulting from use of improper quality water chemicals or cleaning agents detrimental to equipment materials Service calls for improper installation or operating instructions Corrections to any of the following are billable services electrical service connection 2 tubing connections 3 gas regulators 4 gas tanks 5 unit leveling 6 room ventilation 7 adverse ambient temperature fluctuations 8 any repair external to the unit Damage resulting from accident alteration misuse abuse fire flood acts of God or improper installation Repairs to parts or systems resulting from unauthorized unit modifications Any labor costs other than that specified during the parts and labor warranty period which may include additional warranty on CO sensors blower motors water jackets etc Thermo Fisher Scientific Direct Heat C02 Incubator V Thermo Fisher Scientific Table of Contents Installation and Start Up 1 1 ed qu
63. ncubator 3 5 Section 3 Configuration Set New Span Number for T C 0 Sensors Setting a Low RH Alarm Limit 3 6 Direct Heat CO2 Incubator If a new T C sensor is being installed the two numbers on the factory installed sticker on the T C cell must be entered to calibrate the in the unit Note For the technician s convenience a label containing the two numbers on the T C cell is affixed inside the electronics drawer A 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until T CS XXXX shows in the message display 3 Press up down arrow to change the span number to match the sticker 4 Press ENTER to save the setting 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter On units with the RH option installed a low RH alarm limit may be entered The low RH alarm limit is the RH in the cabinet that causes a low RH alarm The setpoint is variable from setpoint 0 to 90 RH The factory setting is 0 RH which disables the alarm 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until RH LO XX shows in the message display 3 Press up down arrow to change the RH low alarm limit 4 Press ENTER to save the RH low alarm limit 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter Thermo Fisher Scientific Section 3 Configuration Enable RH Alarms to The low RH
64. nd are destroyed Unit is not powered off before removing the sensor if applicable Damage to the sensor may result Dummy sensor is not installed The sensor cable cannot withstand sterilization cycle temperatures and is destroyed properly The outer door is opened during the heat or sterilization phases An outer door alarm occurs CLOSE DOOR in the display plus an audible cannot be silenced and visual alarm The outer door is open longer than 20 seconds during phases listed above Cycle is canceled unit goes to CANCELED COOL PHASE The outer door is open during the cool down phase when the temp is 60 C or greater An outer door alarm occurs Power interruption during the HEAT PHASE HEAT PHASE resumes if the chamber temperature was less than 90 C when power was interrupted HEAT PHASE resumes if the chamber temperature was greater than 90 C when power was interrupted and the temperature dropped less than 1 C CANCELED COOL PHASE starts if the chamber temp drop is more than 1 C Power interruption during the STERILIZATION PHASE HEAT PHASE begins if the chamber temperature has not dropped below 139 C STERILIZATION PHASE starts again when the chamber temperature reaches 140 C CANCELED COOL PHASE starts if the chamber temp dropped below 139 C For further information on CANCELED COOL PHASE see page 5 12 Thermo Fisher Scientific Direct Heat C02 Incubator 5 7 Section 5 Routine Maintenance 5 8 Sterilization C
65. o Fisher Scientific Asheville LLC 401 Millcreek Road Marietta Ohio 45750 USA Thermo Laboratory incubator 3542 2007 Release 1 Release Level REL shown on serial tag This product conforms to the following European Union Directive s EMC LVD 89 336 EEC 73 23 This product conforms to the following Harmonized International and National EMC EN 61326 1 1997 Class A EN 50081 1 EN 50082 1 EN 50011 1 97 Standards LVD EN 61010 1 1993 Amendments 1 and 2 CSA C22 2 No 1010 1 UL 61010 1 UL 61010 2 010 Richard L Miller Regulatory Compliance Manager ThermoFisher SCIENTIFIC 25 January 2007 eclaration of Conformity Manufacturer s Name Manufacturer s Address Product Description Product Designations Year of Initial Marking CE Affected Serial Numbers Thermo Fisher Scientific Asheville LLC 401 Milicreek Road Marietta Ohio 45750 USA Thermo Laboratory Incubator 3541 2007 Release 1 Release Level REL shown on serial tag This product conforms to the following European Union Directive s LVO 89 336 EEC 73 23 EEC This product conforms to the following Harmonized international and National Standards EN 61326 1 50081 1 LVD EN 61010 11993 Amendments 1 and 2 CSA C22 2 No 1010 1 UL 61010 1 UL 61010 2 010 Regulatory Compliance Manager SCIENTIFIC 25 January 2007 eclaration of Conformity Manufactu
66. or alarm ringback times Message Display Shows system status Mode at all times Shows CLASS 100 or SYSTEM during normal operation or alarm messages if the system detects an alarm condition see Section 4 Alarms The mes sage CLASS 1007 is a timing mechanism indicating that under normal operating conditions with the HEPA filter installed the air inside the chamber meets the Class 100 air cleanliness standard for particulates of 0 5 micron size or larger per cubic foot of air Further information on the Class 100 classification of air quality is available from Thermo Upper and Lower Displays The 7 segment upper display shows temp and RH The lower display shows CO2 1 Maintain a minimum three inch clearance behind the incubator for electrical and gas hook ups In addition a three inch ventilation space is needed on each side 2 Locate the unit on a firm level surface capable of supporting the unit weight of 260 lbs 118kg 3 Locate the unit away from doors and windows and heating and air conditioning ducts 4 Lift the unit by the sides of the cabinet base Do not attempt to lift it by the front and back This places stress on the outer door hinges Thermo Fisher Scientific Stacking the Thermo Fisher Scientific Incubators Section 1 Installation and Start Up Warning Install stacked units against a wall or similar structure A Warning With incubators in a stacked configuration
67. or lights 3 Press the right arrow until IR CAL XX X appears in the message display 4 Press the up down arrow to adjust the display to match the independent instrument reading 5 Press ENTER to store the calibration 6 Press the MODE key to return to RUN mode Model 3540 and 3541 incubators are equipped with an optional direct readout relative humidity sensor This is a readout only of the chamber relative humidity level It does not provide any control of the relative humidity in the cabinet Relative Humidity Stabilization Times Start Up Allow 12 hours for the relative humidity and temperature in the chamber to stabilize before proceeding Already Operating Allow at least 2 hours after temperature display reaches setpoint for relative humidity to stabilize before proceeding 1 Place an accurate independent instrument in the center of the chamber Allow at least 30 minutes for RH to stabilize 2 Press the MODE key until the CAL indicator lights 3 Press the right arrow key until RH CAL XX appears in the message display 4 Press the up down arrow to match the display to the independent instrument 5 Press ENTER to store the calibration 6 Press the MODE key to return to RUN mode Thermo Fisher Scientific Section 2 Calibration Relative Humidit If a reliable RH measuring device is not available the display can be 5 ntinued calibrated to a typical level 1 Follow the RH sta
68. passes the access code The factory setting is 000 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until ACC CODE shows in the message display 3 Press up down arrow to change the access code 4 Press ENTER to save the access code 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter Thermo Fisher Scientific Set Low Temp Alarm Limit tracking alarm Enable Low Temp Alarm to Trip Contacts Thermo Fisher Scientific Section 3 Configuration The low temp alarm limit is the deviation from the temperature setpoint which causes a low temp alarm The low temp alarm is variable from 0 5 below setpoint to 5 below setpoint The factory setting is 1 below setpoint A minus sign in the display indicates that the alarm setting is below the setpoint 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until TEMP LO X X shows in the message display 3 Press up down arrow to change the low temp alarm limit 4 Press ENTER to save the low temp alarm limit 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter The low temperature alarm can be programmed to trip the remote alarm contacts A setting of ON allows this an OFF setting blocks the low temp alarm from tripping the contacts The factory setting is ON 1 Press the MODE key until the CON indicator lights
69. per performance of the control system A single stage CO pressure regulator will not maintain 15 psig 103 4 kPa to the incubator as the pressure in the CO cylinder decreases therefore a two stage regulator is recommended Direct Heat CO2 Incubator 1 11 Section 1 Installation and Start Up Connecting CO Gas Supply continued Incubator Start Up Setting the Operating Temperature 1 12 Direct Heat C02 Incubator Warning If higher purity is desired inside the incubator greater than 99 596 pure the pressure regulator should be constructed with a stainless steel diaphragm along with specifying the purity of the CO from the gas supplier Follow the manufacturers instructions to ensure proper and safe installation of the pressure regulator on the gas cylinder Consult your facility safety officer to ensure that the equipment is installed in accordance with the codes and regulations that are applicable in your area A The CO gas supply being connected should be industrial grade 99 596 pure and should not contain siphon tubes Install a two stage pressure regulator at the cylinder outlet The high pressure gauge at the tank should have 0 2000 psig range The low pressure gauge at the incubator inlet should have a 0 30 psig range Input pressure to the incubator must be maintained at 15 psig 103 4 kPa The incubator has serrated fittings on the back of the cabinet to connect the gas supply Refer to Figure 1 1
70. purposes only The contents and the product it describes are subject to change without notice Thermo Fisher Scientific makes no representations or warranties with respect to this manual In no event shall Thermo be held liable for any damages direct or incidental arising out of or related to the use of this manual Warning If the incubator is not used in the manner specified in this operating manual the protection provided by the equipment design may be impaired A 2007 Thermo Fisher Scientific All rights reserved Direct Heat C02 Incubator Thermo Fisher Scientific Preface Important operating and or maintenance instructions Read the accompanying text carefully Potential electrical hazards Only qualified persons should perform procedures associated with this symbol Equipment being maintained or serviced must be turned off and locked off to prevent possible injury Hot surface s present which may cause burns to unprotected skin or to materials which may be damaged by elevated temperatures Marking of electrical and electronic equipment which applies to electrical and electronic equipment falling under the Directive 2002 96 EC WEEE and the equipment that has been put on the market after 13 August 2005 24 gt gt This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the WEEE symbol Thermo Fisher Scientific has cont
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72. racted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo s compliance with this directive the recyclers in your country and information on Thermo products will be available at www thermofisher com Always use the proper protective equipment clothing gloves goggles etc v Always dissipate extreme cold or heat and wear protective clothing Always follow good hygiene practices v Each individual is responsible for his or her own safety Thermo Fisher Scientific Direct Heat C02 Incubator iii Preface Do You Need Information or Assistance on Thermo Scientific Products If you do please contact us 8 00 a m to 6 00 p m Eastern Time at 1 740 373 4763 Direct 1 800 438 4851 Toll Free U S and Canada 1 740 373 4189 FAX http www thermo com Internet Worldwide Web Home Page service led marietta thermofisher com Service E Mail Address Our Sales Support staff can provide information on pricing and give you quotations We can take your order and provide delivery information on major equipment items or make arrangements to have your local sales representative contact you Our products are listed on the Internet and we can be contacted through our Internet home page Our Service Support staff can supply technical information about proper setup operation or troubleshooting of your equipment We can
73. rer users must check with the manufacturer that the proposed method will not damage the equipment A Use an appropriate disinfectant All articles and surfaces must be thoroughly cleaned rinsed with sterile water and rough dried Warning Alcohol even a 7096 solution is volatile and flammable Use it only in a well ventilated area that is free from open flame If any component is cleaned with alcohol do not expose the component to open flame or other possible hazard Do not spray the T C sensor with flammable solutions The internal temperature of the sensor is approximately 150 C when the unit is in operation Allow sufficient time for the sensor to cool before cleaning Do not use strong alkaline or caustic agents Stainless steel is corrosion resistant not corrosion proof Do not use solutions of sodium hypochlorite bleach as they may also cause pitting and rusting A HEPA filter replacement kit see Parts List should be on hand prior to cleaning the incubator interior Direct Heat CO2 Incubator 5 1 Section 5 Routine Maintenance Remove the shelves access port filter assembly HEPA filter air sample filter and tubing and the left and right duct sheets Discard the HEPA filters and the access port filter assembly See Figure 5 1 i HEPA Filter K a Access Air Sample Port Filter Filter 7 and stopper and tubing assembly Humidity Pan Figure 5 1 Component Locations 2 Wash t
74. rer s Name Manufacturer s Address Product Description Product Designations Year of Initial Marking CE Affected Serial Numbers Thermo Fisher Scientific Asheville LLC 401 Millcreek Road Marietta Ohio 45750 U S A Thermo Laboratory Incubator 3543 2007 Release 1 Release Level REL shown on serial tag This product conforms to the following European Union Directive s EMC LVD 89 336 EEC 73 23 This product conforms to the following Harmonized International and National Standards EMC EN 61326 1 1997 Ape A EN 50081 1 EN 50082 1 EN 50011 1 97 LVD EN 61010 1 1993 Amendments 1 and 2 CSA 22 2 No 1010 1 UL 61010 1 UL 61010 2 010 Richard 1 2 Regulatory Compliance Manager ThermoFisher SCIENTIFIC 25 January 2007 Thermo Scientific Controlled Environment Equipment 401 Millcreek Road Marietta Ohio 45750 United States www thermofisher com
75. revious parameter De SCROLL FOR PARAMETERS save the setting Numbers decrease 2 6 Direct Heat C02 Incubator Thermo Fisher Scientific Turn Audible Alarm ON OFF New HEPA Filter Thermo Fisher Scientific Section 3 Configuration Several features available in Configuration mode allow custom setup of the incubator These features are listed with descriptions below All features may not be necessary in all applications but are available if needed To enter Configuration mode press the MODE key until the CON indicator lights Press the right and or left arrow until the appropriate parameter appears in the message display See Chart 3 1 for more detail The audible alarm can be turned on or off The factory setting is ON 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until AUDIBLE XXX shows in the message display 3 Press the up down arrow to toggle AUDIBLE ON OFE 4 Press ENTER to save the setting 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter When the REPLACE HEPA reminder displays and the visual alarm flashes the specified time has elapsed and the HEPA filter should be replaced To clear the display and reset the timer after replacing the HEPA filter with a new one follow the steps below 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until NEW HEPA shows in the message di
76. splay 3 Press ENTER to restart the timer and clear the REPLACE HEPA alarm 4 Press the MODE key to return to RUN mode Direct Heat CO2 Incubator 3 1 Section 3 Configuration 3 2 Set REPLACE HEPA filter reminder Set Access Code Direct Heat CO2 Incubator A HEPA filter replacement timer can be set for a specific amount of time from 1 to 12 months of actual unit running time Time will not accrue when the unit is turned off The default time is 6 months When the allotted time runs out REPLACE HEPA appears in the display and the visual alarm flashes set the reminder use the following procedure 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until REPL HEPA is displayed 3 Press the up down arrow to choose the number of months desired 4 Press ENTER to save the number 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter Note After the reminder has been set check the allotted time remaining by going to Configuration mode then pressing the right arrow until NEW HEPA XXX displays This number is the remaining days before the filter replacement time specified runs out For example if 12 months was chosen in the REPL HEPA XX message display the NEW HEPA number would be 365 days A 3 digit Access Code can be entered to avoid unauthorized personnel from changing the setpoints calibration or configuration A setting of 000 by
77. t cloth Some precautions in the cleaning and care of the incubator glass doors Moisture leaches alkaline materials sodium Na from the surface of the glass Evaporation of the moisture concentrates the alkaline and may produce a white staining or clouding of the glass surface Cleaning chemicals with a PH above 9 and heat autoclaving accelerate the corrosion process Therefore it is very important to rinse and dry the glass doors after cleaning Autoclaving the glass doors should be avoided There is no simple method for repairing corroded glass In most cases the glass must be replaced Clean Humidity Pan Clean the humidity pan with soap and water and a general use laboratory disinfectant Rinse with sterile water and spray with 70 alcohol The pan may be autoclaved HEPA Filter Replace the HEPA filter when the REPLACE HEPA reminder is Maintenance displayed The REPLACE HEPA reminder can be set to alarm after a specified time from 1 to 12 months The reminder default is the factory recommended setting of 6 months For details see Section 3 Replace Power Fuses access the only replaceable fuse in the incubator Thermo Fisher Scientific 1 Turn off the incubator s power switch and unplug the power cord 2 Remove the two screws from the top of the control panel Grasp the control panel on each side and pull straight outward 4 Figure 5 3 shows the location of the fuse See Table 5 1 following for replacement f
78. t or your local distributor The microprocessor in Series 8000DH incubators continually scans all available sensors to ensure that they are operating properly Should an error be detected the incubator sounds an alarm and shows the appropriate message Contact your local distributor Thermo Fisher Scientific anssn 105 sn 100 oq 10 911915 sq o 105025 TON UO Burderds e 8 sey Aue 10 yooq osn TON e Te 205 sdr y poumboz suonrpuoo sn uodn 3uopuodop speA1o3ut 3uounsn pe pue Surueo MO 9q Parisi Joquinu W sn 3283002 oseo d sonueire A 10 22utuo3ure A UO 104 seore utui UT o qe reAe IGL SIN Sursn sueroruqooa 201 125 10 enueur Jo 5 226 oAnuoAo1d Jo 151 posuopuoo e 51 1 Vms Aq pue poxooupo st ar pue 104 751584
79. t the proposed method will not damage the equipment Accidental spills of hazardous materials on or inside this unit are the responsibility of the user Thermo Fisher Scientific Shelf Installation Thermo Fisher Scientific 1 Section 1 Installation and Start Up Install the large sheet metal side ducts with the tabs facing into the center of the chamber with their slots up There are no right side or left side ducts simply rotate one of them to fit the opposite side Tilt the side ducts as they are placed into the chamber so the tops fit into the top air duct then guide them into the vertical position Figure 1 10 shows the side duct as it would be oriented for the right side of the chamber Side toward side duct Shelf Channels rr Side Duct Side toward shelf gt R Eum d P Ed Shelf channel Side duct Shelf channel rear slo tab front slot Aux Side duct Right Side Duct ight Side Duc se with Shelf Channel Installed E Figure 1 10 Shelf Channels and Side Ducts Figure 1 11 Channels and Slots 2 Referring to Figure 1 10 note that there is no difference in the left and right shelf channels Install the shelf channels by placing the channel s rear slot over the appropriate rear tab on the side duct Pull the shelf c
80. umbers increase Operating to CALIBRATE TEMP XXXC Press Enter Temperature mode to save setting Press to return Numbers decrease to previous parameter 0 Numbers increase OTEMP Press Enter Temperature to save setting Press lt 1 to return Numbers decrease to previous parameter SCROLL FOR PARAMETERS Numbers increase Percent CO 02 XXX to save setting Press lt Q to return Numbers decrease to previous parameter Thermo Fisher Scientific Direct Heat CO2 Incubator 1 15 Section 2 Calibration After the unit has stabilized several different systems can be calibrated In Calibration mode the air temperature CO2 and RH levels can be calibrated to reference instruments To access Calibration mode press the MODE key until the CAL indicator lights Press the right and or left arrow until the appropriate parameter appears in the message display See Chart 2 1 at the end of this section for more detail Calibration frequency is dependent on use ambient conditions and accuracy required A good laboratory practice would require at least an annual calibration check On new installations all parameters should be checked after the stabilization period Prior to calibration the user should be aware of the following system functions While the unit is in Calibration mode all system control functions are stopped so the unit remains stable Readout of the system being calibrated appears on the message disp
81. ument 3 Press the MODE key until the CAL indicator lights 4 Press the right arrow until CO2 CAL XX X is displayed in the message display 5 Press the up down arrows to change the display to match the independent instrument 6 Press ENTER to store the calibration 7 Press the MODE key to return to RUN or the right or left arrows to go to the next previous parameter Models 3542 and 3543 have an infrared IR sensor Infrared CO sensors are not effected by chamber atmosphere temperature or humidity However the light detector in the sensor is effected by wide temperature changes Therefore changing temperature setpoints could necessitate a recalibration of the CO Chamber temperature should be allowed to stabilize before checking CO concentrations with an independent instrument especially on start up IR CO Sensor Stabilization Times Start Up Allow the temperature and the CO of the cabinet to stabilize at least 12 hours before proceeding Presently Operating Allow CO to stabilize at least 2 hours at setpoint before proceeding Direct Heat C02 Incubator 2 3 Section 2 Calibration 2 4 Infrared System continued Relative Humidity Direct Heat C02 Incubator me Measure the CO concentration in the chamber through the gas sample port with a Fyrite or other independent instrument Several readings should be taken to ensure accuracy 2 Press the MODE key until the CAL indicat
82. urn 1621 Numbers decrease to previous parameter Low CO Alarm Limit Numbers increase Press Enter to High CO Alarm Limit CO2 save the setting Press lt 1 to return 191 Numbers decrease to previous parameter A C02 alarm relay ON Press Enter to CO Alarm Relay CO2RLY XXX Q save the setting Press lt to return 1621 02 alarm relay OFF to previous parameter Numbers increase Press Enter to T C CO Zero Number T C Z ID save the setting Press lt q to return 1621 Numbers decrease elaine deen 12 11 MEM Numbers increase Press Enter to Co Span Number T C S save the setting Press lt to return 60 Numbers decrease to previous parameter Numbers increase Press Enter to save the setting Press lt 1 to return QD to previous parameter sca Numbers decrease RH Low Alarm Limit e RHalarm relay ON DOO Press lt to return 101 RH alarm relay OFF to previous parameter RH Alarm Relay refer to next page Thermo Fisher Scientific Direct Heat C02 Incubator 3 9 Section 3 Configuration refer to previous page To Configure Display Temperature Display Humidity Press lt 1 toreturn to previous parameter Press lt toreturn to previous parameter DISP RH Chart 3 1 Configuration Page 3 of 3 2 DOO i SCROLL FOR PARAMETERS Display temperature ON
83. use specifications 5 Secure the control panel and return the unit to service If the fuse blows after restoring power to the incubator contact your local distributor Table 5 1 Fuse Replacement Chart Fuse Voltage Manufacturers P N Amperage Rating Rupture Speed IEC Letter Code 230VAC BUSS GMC 0 5A 500mA Time Lag T 115VAC BUSS GMC 1 0A 1 0 Amp Time Lag T Direct Heat CO2 Incubator 5 3 Section 5 Routine Maintenance Replace Sample 1 Connect one end of the air sample filter to the hose barb on the Filter chamber ceiling 2 Connect the other end of the filter to the metal tubing on the HEPA filter adapter Figure 5 2 M Air Sample Filter Figure 5 2 Connect to Metal Tubing Electronics Section 1 Turn off the incubators power switch and unplug the power cord 2 Remove the three screws from the back of the cabinet top 3 Slide the top backward about an inch and lift it off 5 4 Direct Heat CO2 Incubator Thermo Fisher Scientific Thermo Fisher Scientific Section 5 Routine Maintenance Major Components Refer to Figure 5 3 Some components shown are factory installed options Front of incubator Power DC power supply switch Fuse Analog Board is E 4 20 0 1V 0 5V Micro Board Blower Motor Component fan sensor 5 IR or T C thermostat Sample port Overtemp thermostat into RH board IR CO Coo al sensor injection tub
84. y Warning High concentrations of gas can cause asphyxiation OSHA Standards specify that employee exposure to carbon dioxide in any eight hour shift of a 40 hour work week shall not exceed the eight hour time weighted average of 5000 PPM 0 5 CO2 The short term exposure limit for 15 minutes or less is 30 000 ppm 396 CO Carbon dioxide monitors are recommended for confined areas where concentrations of carbon dioxide gas can accumulate A Warning This incubator is designed to be operated with CO gas only Connecting a flammable or toxic gas can result in a hazardous condition Gases other than CO should not be connected to this equipment CO gas cylinders have a UN1013 label on the cylinder and are equipped with a CGA 320 outlet valve Check the gas cylinder for the proper identification labels The CO gas supply being connected to the incubator should be industrial grade 99 596 pure Do not use gas cylinders equipped with siphon tubes A siphon tube is used to extract liquid CO2 from the cylinder which can damage the pressure regulator Consult with your gas supplier to ensure that the CO cylinder does not contain a siphon tube Gas cylinders should also be secured to a wall or other stationary object to prevent them from tipping two stage CO pressure regulator is required to be installed on the outlet valve of the gas cylinder Input pressure to the incubator must be maintained at 15 psig 103 4 kPa for pro
85. ycle Warning The Sterilization Cycle heats the incubator interior surfaces to Direct Heat CO2 Incubator 140 C Contact with any surface inside the outer door during this cycle may result in burns 1 Empty the water from the humidity pan and place the pan back into the incubator Remove any samples instruments dishes etc from the chamber 2 Press and hold the large white sterilization cycle button on the right side of the unit Figure 5 4 for approximately 3 seconds until the LED lights Sterilization Cycle Button Figure 5 4 Sterilization Button Location 3 ENTER the access code if applicable An access code is recommended to prevent accidental cycle initiation 4 Pre Sterilization with T C sensor The display toggles between REMOVE HEPAs REMOVE WATER and PRESS ENTER If ENTER is not pressed within 1 minute the display returns to SYSTEM 5 Remove the HEPA filter air sample filter and access port filter assembly Figure 5 5 Tubing can remain in the unit during the sterilization cycle In addition remove the temp RH recorder probe and IR sensor if applicable Discard the filters Temp RH recorder probe m gt sensor HEPA idi plate Filt Access Temp sensor Port Filter Air Sample inserted into and stopper Filter HEPA collar from assembly behind top duct Figure 5 5 Filter Locations Pre Sterilization with
86. zation Cycle and before is allowed to enter the cabinet 1 and install the humidity pan 2 Allow to stabilize at the desired operating temperature for at least 12 hours 3 Calibrate the display to 0 0 Note If the Cool Phase is canceled the display toggles between CYC CANCELED and COOL PHASE e With sensor When the temperature cools down to the original set operating temperature or 30 C whichever is higher the display toggles between CYC CANCELED REPL HEPAs and PRESS ENTER The white LED is lighted but no longer flashes e With IR CO sensor When the temperature cools down to the original set operating temperature or 30 C whichever is higher the display toggles between CYC CANCELED POWER OFF and REPLACE IR The white LED is lighted but no longer flashes After the unit is powered back on with the IR sensor reinstalled the display toggles between CYC CANCELED REPL HEPAs and PRESS ENTER Thermo Fisher Scientific Remote Alarms Thermo Fisher Scientific Section6 Factory Options A description of the factory option connections to external equipment follows A set of relay contacts is provided to monitor alarms through an RJ11 telephone style connector on the back of the cabinet Refer to Figure 6 5 for the location of the alarm connector The 12 foot telephone cord P N 190388 and RJ11 to screw terminal conversion box P N 1

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