Pump P 2.1S/P 4.1S User Manual
Contents
1. Procedure Procedure Result Prerequisite Procedure Note Procedure KNAUER Initial Startup Computer is on There is a patch cable for each device and the computer 1 Use the patch cable to connect the router and the computer Repeat this step to connect all devices 2 Use the power supply to connect the router to the mains power system Configuring the Router The router is preset at the factory You can find a label at the bottom side of the router on which IP address user name and passwort are printed These information help to open the router configuration 1 To open the router configuration start your Internet browser and enter the IP address not for all routers 2 Enter user name and passwort 3 Configure the router as DHCP server 4 In the router configuration check the IP address range and make changes if necessary Once the router has assigned IP addresses to all devices the chromatography software can be used to remotely control the system Integrating the LAN into a Company Network A network administrator can integrate the LAN into your com pany network In this case you use the WAN port of the router There is a patch cable for the connection 1 Check that the IP address range of the router and of the com pany network do not overlap 2 In case of an overlapping change the IP address range of the router 3 Use the patch cable to connect the router WAN port to th2. Is the patch cable connected correctly to the router and the computer Check the router settings Is the router set to DCHP server Is the IP address range sufficient for all the connected devices Check all connections Are the patch cable connected to the LAN ports and not the WAN port Are all cable connections between devices and router correct Are the cables plugged in tightly If the router is integrated into a company network pull out the patch cable from the WAN port Can the devices communicate with the computer even though the router is disconnected from the com pany network In case you own a Control Unit check the settings in the menu Setup gt Network Is LAN DHCP set for controlling Did the device receive an IP address Turn off all devices router and computer Firstly turn on the router secondly the devices and the computer Has this been successful Replace the patch cable to the device with that no connection could be established Has this been successful KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 18 KNAUER Initial Startup Pin Header for Remote Control CAUTION Electrostatic discharge can destroy the electronics Wear protective bracelet against electrostatic discharge and ground Plug Connector Assignments Contact data Explanation GROUND Reference point of the voltage at the signal inputs START IN TTL compatible input min 10 mA Low
3. Manufacturer Wissenschaftliche Ger tebau Dr Ing Herbert KNAUER GmbH Hegauer Weg 38 14163 Berlin Germany Phone 49 30 809727 0 Fax 49 30 8015010 E Mail info knauer net Internet www knauer net Transportation Damages The packaging of our devices provides the best possible protec tion against transportation damage Check the devices for signs of transportation damages In case you notice any damage con tact the technical support and the forwarder company within three workdays Pump P 2 1S P 4 15 User Manual V6870A version 1 1 44 Disposal AVV marking WEEE registration KNAUER Disposal Drop off old devices at the certified waste facilities where they will be disposed of properly According to the German Abfallverzeichnisverordnung AVV January 2001 old devices manufactred by KNAUER are marked as waste electrical and electronic equipment 160214 KNAUER as a company is registered by the WEEE number DE 34642789 in the German ElektroAltger teRegister EAR It belongs to category 8 under which fall all medical devices and laboratory equipment Within the meaning of the WEEE directive all distributors and importers are responsible for the disposal of old devices End users can send their old devices which must have been manu factured by KNAUER back to the distributor the importer or the company free of charge but would be charged for their dis posal Pump P 2 1S P 4 15 User Manu
4. Power Supply and Mains Connection 19 Labeling on the Pump Heads ces sure SA RARAS Bales 19 Connecting the Eluent Line to the Pump Head 20 Switching on the Pump cis e E a 20 Operation caw nc Aa ES 21 Operating with the Keypad iure a oe ean a aed 21 Setting the FlOW Rate cee ex sete ee eee pee Seis eee De a nie 21 Setting the Pressure Switch Off 22 Setting the Power Consumption 23 Flushing the Pump 224 snvequcte awed AAA Sake eae ad 24 Flushing the Pistons serere roi DARA ee ees dats 25 KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 4 Table of Contents External Control ca ia cias 26 Controlling the Flow Rate su rennes set sue tan Rush ess 27 Starting with a Short Circut ss iva se we ars a oe we S aoe 28 Starting Directly after Connecting to Power Supply 28 Control with Chromatography Software 29 Local Area Network LAN Sr da rs A as ares 29 RS 232 POTE LES bu men Us Ars Melee Stitch le a D ables 30 Installation Qualification IQ 31 Operation Qualification OQ 31 WESC INUSIVAS cad ste nai nee Bede RE Re 22 ea 31 EXGCULION Pirate s ictal e A dels at tak tote ee ee a a 31 Maintenance and Care 32 Switching Off the PUMP 3 x65 Sen cae A as 32 Co
5. formit Europ enne which is confirmed by the Declaration of Conformity High voltage hazard Electrostatic discharge hazard Wear protective bracelet against electro static discharge and ground Pull the power plug Observe torque of 5 nm maximum Wear protective gloves KNAUER Pump P 2 1S P 4 1S User Manual V6870A version 1 1 12 Unpacking and Setup Unpacking and Setup Phone Fax E mail Power supply Requirements KNAUER Contacting the Technical Support You have various options to contact the technical support 49 30 809727 111 49 30 8015010 support knauer net Location Requirements The device is only suitable for 24 V direct current The supplied power supply unit is to be used to connect the device to the power unit The location for the device must meet the following require ments Weight 1 5 kg Dimensions 105 x 100 x 185 mm Width x Height x Depth Power supply 24V DC Air humidity lt 90 Temperature 4 40 C 39 2 104 F Space require Side clearance to other devices ments If there is a device on one side minimum clearance of 5 cm f there are devices on both sides minimum clearance of 10 cm At least 30 cm gap to the fan on the rear of the device CAUTION Defect of the device due to overheating Set up the device so that it is protected against exposure to direct sunlight Make sure the room is well ventilated Allow 30 cm space at t
6. orientation When assembling the pump head the piston rods must be inserted on the same side they have been removed from Unscrew inlet fitting and outlet fitting Unscrew the inlet fitting to the eluent Unscrew the outlet fitting to the pressure sensor Alternately unscrew the 4 fastening screws Hold the pump head and consecutively pull out all fastening screws Pump P 2 1S P 4 15 User Manual V6870A version 1 1 34 Maintenance and Care Installing the Pump Head CAUTION Damage to the pump head caused by strongly tightened capillary fittings Check the torque of screw fittings 5 Nm for stainless steel fittings 0 5 Nm for PEEK fittings 1 Insert the fastening screws and tighten alternately Tighten the outlet fitting to the pressure sensor Tigthen the inlet fitting to the eluent Tighten the inlet fitting and the outlet fitting O of the piston Exchanging the Check Valves Dirty check valves do not open and close properly They cause pressure fluctuations and irregular flow Note Insert the valves in the direction of flow Legend O Ball Flow direction Abb 20 Check valve KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 35 Maintenance and Care Removing the Check Valves Note Loosen the screw fittings of the capillary connections alternately to prevent the capillaries from bending Prerequisite Pump head has been
7. 30 809727 111 Fax 49 30 8015010 support knauer net manufacturer Proper maintenance of your HPLC device will ensure successful analyses and reproducible results CAUTION Intruding liquids can cause damage to the device Place solvent bottles next to the device or on a solvent tray Moisten the cleaning cloth only slightly All smooth surfaces of the device can be cleaned with a mild commercially available cleaning solution or with isopropanol Maintenance Contract The following maintenance work on the device may only be per formed by KNAUER or a company authorized by KNAUER and is covered by a separate maintenance contract e Opening the device or removing housing parts What maintenance tasks may users perform on the device Users may perform the following maintenance tasks themselves Replacing the pump head Exchanging the check valves Pump P 2 1S P 4 15 User Manual V6870A version 1 1 33 Legend O Capillary fitting piston backflushing Fastening screw Inlet fitting eluent Outlet fitting pressure sensor Requirements Procedure KNAUER Maintenance and Care Replacing the Pump Head WARNING Aggressive or toxic solvent residue can irritate the skin Wear protective gloves Removing the Pump Head The pump head has been flushed with suitable solvent CAUTION Piston rods can break Before disassembling the pump head remove the two piston rods and deposit in the correct
8. active After receiving a signal from an exter nal device the device starts If con trolled with software an electronic trigger is send through the LAN ERROR IN OUT TTL compatible input min 10 mA Low active After receiving a signal e g short cir cuit to ground from an external device an error message appears and the device stops ANALOG IN Flow rate is controlled through exter nal control voltage 0 10 V ANALOG OUT Analog output signal for reproducing the measured system pressure Ground CAUTION Electronic hazard when using an identically con structed power adapter from another manufac turer Before establishing a power connection call the Technical Support The ground connection for the pump has a designated hole with a thread M3 on the back of the device f the supplied power adapter is used then the ground con nection remains unused Please contact the technical support of KNAUER if the pump along with other devices should be connected to the power supply with a 6 prong power adapter a pump needs to be grounded exclusively Pump P 2 1S P 4 15 User Manual V6870A version 1 1 19 Initial Startup Power Supply and Mains Connection CAUTION Electronic hazard if the power adapter is turned on while connecting or interrupting the power connection Switch off the power adapter beforehand The device is intended for use with AC power networks of 100 240 V Pump The supplied powe
9. consumption Fig 13 Minimum power con sumption Hold down left arrow key Press right arrow key once When the cursor flashes use the arrow l O 0 0 keys again to set the value for the minimum power consumption Press start stop key once to return to the start display Fig 14 Maximum power con sumption The setting is completed If the value for maximum power consumption is exceeded the pump switches off Flushing the Pump The display shows vertical arrows while the pump is flushing The flow rate can be changed while being purged The change takes effect immediately Pump with pressure sensor Venting screw of the pressure sensor is open Syringe is connected to venting capillary Pump without pressure sensor Blind fitting in the outlet to the pressure sensor is removed Syringe is connected to the capillary on the pump head out let 1 Prime liquid with the syringe 2 Hold down start stop key until flushing starts Pump P 2 1S P 4 15 User Manual V6870A version 1 1 25 Operation Flushing the Pistons When you flush the pistons regularly the service life of the seals and pistons increases While flushing contaminants are washed from the rear piston area Flushing solution The following solvents are recommended for flushing the col umns Water Mixture of 80 water and 20 ethanol Isopropanol Procedure 1 Connect the outlet to the waste contain
10. flushed Procedure 1 Unscrew the inlet fit ting O and the outlet fitting Remove the check valve Unscrew the inlet fitting to the eluent and remove the check valve Abb 21 Position check valve Cleaning the Check Valves 1 Fill a beaker with solvent 2 Place the valve in the beaker 3 Put the beaker in an ultrasonic bath for at least 10 minutes Installing the Check Valves Tools Torque wrench Procedure 1 Insert the check valve and tighten the inlet fitting to the eluent Insert the check valve Tighten the inlet fitting O and the outlet fitting Using the torque wrench tighten the outlet fitting 2 and the inlet fitting with 7 5 Nm Abb 22 Position check valve KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 36 Troubleshooting Troubleshooting First measures for troubleshooting Check all screw fittings Check whether air has gotten into the supply lines Check device for leaks Further measures Check errors against error list Contact the technical support of KNAUER Error List and Solutions Problem soie Pump will not turn Power cable needs to be connected to on power supply and power adapter has to be turned on e Inspect the power cable to ensure that it is plugged into the power supply When purging the The venting screw on the pressure sen pump switches off sor must be turned up e Check if the ventin
11. head APG90DA stainless steel Pumpe P 2 15 mit 50 ml Pumpenkopf APG90DC Titan Pump P 2 1S P 4 15 User Manual V6870A version 1 1 42 Accessories and Spare Parts Available Pump Heads fame Order mer 10 ml pump head with titanium inlay A54121 1 10 ml pump head with stainless steel A54123 1 inlay 10 ml pump head with ceramic inlay A54102 50 ml pump head with titanium inlay A54131 50 ml pump head with stainless steel A541 33 inlay KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 43 Legal Information Legal Information KNAUER Warranty Conditions The factory warranty for the device is valid for 12 months after the date of dispatch All warranty claims shall expire in the event that any unauthorized changes are made to the device During the warranty period any components with material or design related defects will be replaced or repaired by the manu facturer free of charge This warranty excludes the following 1 Accidental or willful damage 2 Damage or errors caused by third parties that are not con tractually related to the manufacturer at the time the damage occurs 3 Wear parts fuses glass parts columns light sources cuvettes and other optical components 4 Damage caused by negligence or improper operation of the device and damage caused by clogged capillaries 5 Packaging and transport damage In the event of device malfunctions directly contact the manu facturer
12. 15 User Manual V6870A version 1 1 38 Technical Data Technical Data Conveying system Dual piston pump with main and auxiliary piston Flow rate range 10 ml pump head 0 001 10 ml min 50 ml pump head 0 01 50 ml min Maximum pressure 10 ml pump head 40 MPa to 10 ml min Imax 70 50 ml pump head 15 MPa to 50 ml min Imax 80 Flow rate accuracy 1 measured at 5 50 of flow range using ethanol water 10 90 For pumps without a pressure sensor dependent on pressure Flow rate precision Relative standard deviation RSD lt 0 5 1 ml min Gradients Isocratic HPLC pump Expandable to a binary high pressure gradient system con trolled by software System protection Pump with pressure sensor Pinin and Pmax adjustable limin and Imax adjustable Pump with pressure sensor Imin and lmax adjustable Operation LAN RS 232 Remote terminal strip Buttons on the device Pump with integrated 100 240 V power unit Supply voltage range Pump with integrated 50 60 Hz power unit Supply frequency KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 39 Technical Data Pump with external 24 V 50 VA power adapter and power cable Power supply Active power con maximum 40 W sumption IP protection class IP 20 Weight Pump without pressure sensor 2 3 kg Pump with pressure sensor 2 4 kg Dimensions including Pump without pressure sensor components 220 x 121 x 138 1 mm length x wi
13. N cable Local Area Network LAN A device connected to a LAN is recognized by the software and automatically receives an IP address because it is set to Dynamic Host Configuration Protocol DHCP at the factory Device has been connected to LAN Status display is active 1 Press both arrow keys simultaneously until the correct display appears 2 Let go of the arrow keys Fig 18 Display control 3 Using the arrow keys scroll until LAN When setting is finished the status display appears Pump P 2 1S P 4 15 User Manual V6870A version 1 1 30 Operation RS 232 Port Prerequisite Interface RS 232 is connected Status display is active Procedure 1 Press both arrow keys simultaneously until the correct display appears RISE 2 312 Let go of the arrow keys Fig 19 Display control Using the arrow keys scroll until RS 232 is displayed Result When setting is finished the status display appears KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 31 Installation Qualification IQ Installation Qualification IQ Installation report Certification on the functionality of the device During installa tion of the device an installation report IQ document is cre ated upon request in coordination with the technical support of the manufacturer This installation report needs to be completed in full and signed by both parties It serves as proof of the properly executed install
14. PRURA gt Pump P 2 1S P 4 15 User Manual V6870A Il KNALIER 3 Table of Contents Table of Contents Note For your own safety read the manual and always observe the warnings and safety information on the device and in the manual Table of Contents s essieu de oa diet e 3 Intended USE ia E eee s Bee 5 DEVICE OVERVIEW er nn Peete Se oem ed dns de Shaws Bares 5 Features ane ET RAS ARA AA gece 6 lH NES aia ca 7 SCOP Of Deliver A A YE 8 SM A NAS ROS STA ne ee 9 Definition of Personal and Material Damages 10 Symbols and SONS dar 11 Unpacking and Setup std rad es ee men Se Rees 12 Contacting the Technical Support 12 Location Requirements soda das POP drone 12 Unpacking Se ts A SAA A eds 13 Initial START RARE 14 Ports on the Rear Side ice Diada 6a E oh AAA AAA ad 14 Controlling with a Computer in a Local Area Network LAN 14 Configuring the LAN Settings 0 000 e A AAA AA 15 Connecting the Cables 20 0 ds 15 Configuring the Router atar TRI ai a 16 Integrating the LAN into a Company Network 16 Controlling Several Systems Separately ina LAN 16 Troubleshooting for Connection Problems 17 Pin Header for Remote Control 18 Plug Connector Assignments 18 A A a aus oe E Es iil ns Oe Sot ot DE SLA eh bli te NA 18
15. R INOUT ANALOG IN ANALOG OUT Electrostatic discharge Fig 3 Pump rear view External devices like computers fraction collectors etc can be connected in 3 different ways Connected to LAN within a network Connected to RS232 alternately to LAN connection Control with pin header Controlling with a Computer in a Local Area Network LAN This chapter describes how to set up a local area network LAN and how a network administrator can integrate this LAN into your company network The description applies to the operating system Windows and all conventional routers To set up a LAN we recommend to use a router The following steps are necessary 1 On the computer go to the control panel and check the LAN properties 2 Hook up the router to the devices and the computer 3 On the computer configure the router to set up the network 4 Install the chromatography software from the data storage device 5 Switch on the device and run the chromatography software Pump P 2 1S P 4 15 User Manual V6870A version 1 1 15 Prerequisite Procedure Note Legend Modules Router LAN port WAN port Workstation KNAUER Initial Startup Configuring the LAN Settings The LAN uses only one server which is normally the router from that the devices automatically receive their IP address In Windows power saving hibernation standby and screen saver must be deactived n case yo
16. RTLEVEL 0 in the terminal program to make the pump operate during short circuit The description is considered for Windows XP The pump is connected to the power supply 1 Windows Start gt All Programs gt Accessories Communica tions gt HyperTerminal 2 Connect the pump using a LAN or RS 232 3 Enter STARTLEVEL 0 Starting Directly after Connecting to Power Supply By default the pump is stopped and started using the start stop button The STARTMODE setting allows you to start the device right after connecting it to power supply STARTMODE O default setting The pump does not start operating right after being connected to power supply STARTMODE 1 The pump starts operating right after being connected to power supply Pump P 2 1S P 4 15 User Manual V6870A version 1 1 29 Prerequisite Prerequisite Procedure Result KNAUER Operation Enter in the terminal program using RS 232 STARTMODE 1 so that the pump is in operation directly after being connected to the power supply The description is considered for Windows XP The pump is connected to the power supply 1 Windows Start gt All Programs Accessories Communica tions gt HyperTerminal 2 Connect the pump using a LAN or RS 232 3 Enter STARTMODE 1 Control with Chromatography Soft ware To be able to control the pump using chromatography software the computer must be connected to the device either with a RS232 cable or a LA
17. al V6870A version 1 1 45 Declaration of Conformity Declaration of Conformity Manufacturer name and address AZURA Pump P 2 15 AZURA Pump P 4 15 Wissenschaftliche Ger tebau Dr Ing Herbert KNAUER GmbH Hegauer Weg 38 14163 Berlin Germany Product numbers DPG90AA DPG90AC DPG90DA DPG90DC DGP90BB Product numbers DPG20AA DPG20AC DPG20DA DPG20DC DPG20BB The device complies with the following requirements and prod uct specifications DIRECTIVE 2006 42 EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 17 May 2006 on machinery and amending Directive 95 16 EC recast IEC 60799 1998 Electrical accessories Cord sets and inter connection cords IEC 61010 1 2010 Corrigendum 2011 Safety require ments for electrical equipment for measurement control and laboratory use Low voltage directive 2006 95 EC EN 61000 3 2 2005 A1 2008 A2 2009 Electromagnetic compatibility EMC Part 3 2 EMC standard 2004 108 EC IEC 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements EN 61326 1 Corrigendum 2 2011 Directives for an environmentally sound use of electrical and electronic equipment RoHS directives 2002 95 EC 2003 and 2011 65 EU on the restriction of the use of certain hazardous substances in electrical and electronic equipment WEEE directive 2002 96 EC 2003 on waste electrical and electronic equipment The product was tested with a typical
18. ation and the functionality of the device Operation Qualification OQ Extensive Extensive test of the detector s functionality A successfully exe functionality test cuted OQ ensures that the detector functions properly Test Intervals Run the device test at the following time intervals Average useful life 1 to 5 days week Every 6 months More than 5 days week or 24 hours day Every 3 months Operation with buffer solutions or other salt Every 3 solutions months Execution The execution is done either by the manufacturer s technical ser vice or by a technical service authorized by the manufacturer The OQ documentation required for executing the OQ is with costs once and can be ordered separately from the manufac turer KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 32 Maintenance and Care Maintenance and Care Contact data Technical Support European hotline E mail contact KNAUER Proper maintenance of your HPLC device will ensure successful analyses and reproducible results Switching Off the Pump If you want to switch off the pump for a longer term flush the pump head with isopropanol Contacting the Technical Support If you have any technical questions regarding the hardware or software of the manufacturer please use one of the contact options below Technical Support Hotline Languages German and English Available by telephone 8 am to 5 pm CET Phone 49
19. configuration Berlin 2013 04 15 Ale Dr Alexander Bunz Managing Director The mark of conformity has been applied to the rear panel of the device CE KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 Table O Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig f Fi Ore T ENTS DOr 10 11 12 13 14 15 16 17 18 19 20 21 22 46 Table of Figures gures Pump 2 1S with 10 ml pump head 5 Pump 4 15 with 10 ml pump head 5 PUNID TER VIEW La E ES AAA AA 14 Cabling system LAN ira 15 Labeling on the pump heads 19 Connect the eluent line to the pump head wins baila A ES Vee al NON 20 Status CIS DIVERS en doive 21 PUT OW KEYS ones tu s ss a cd 21 Start STORE sie A nas cer Rain eth ane 21 Display control uscar se as ed Ms die SU 22 M xiMm mM pressure palin iia dabas a 22 Minimum pressure os das rd ts a OR 23 Minimum power consumption 24 Maximum power consumption 24 Flushing rear piston area se dl sas dent ess 25 Status display ria D el Signy Sun a ang 28 Status display ssis aaa a ea as ae Ae amen 2 28 Display Control att rd ne ne a a i ots 29 Display Control ies res RA wee di 30 Check Valve anae rs ad ce a ately Erie dll 34 Position check valve ur spice da OLR tuo
20. d by KNAUER Good Laboratory Practice GLP For development of methods and validation of devices Pro tocol for the Adoption of Analytical Methods in the Clinical Chemistry Laboratory American Journal of Medical Technol ogy 44 1 pages 30 37 1978 Accident prevention regulations published by the accident insurance companies for laboratory work More safety relevant information is listed in alphabetical order in the following table Decontamination Flammability Leak sensor Solvent tray KNAUER Contamination of devices with toxic infectious or radioactive substances poses a hazard for all persons during operation repair sale and disposal of a device All contaminated devices must be properly decontaminated All materials or fluids used for decontamination must be collected separately and dis posed of properly Organic eluents are highly flammable Since capillaries can detach from their screw fittings and allow eluent to escape it is prohibited to have any open flames near the analytical sys tem Pay attention to messages from the device or the chromatog raphy software Risk of electrical shock or short circuit if liquids get into the device s interior For this reason place all bottles in a solvent tray Pump P 2 1S P 4 15 User Manual V6870A version 1 1 10 Safety Eluent lines Install capillaries and hoses so as to liquids can not get into the interior in case of a leak Power strip If s
21. d individually for each pump The flow rate can be altered while the pump is in operation Pump P 2 1S P 4 15 User Manual V6870A version 1 1 22 Operation Practical tip Hold down both arrow keys to expedite changing the values Procedure 1 Use the arrow keys to set a value for the flow no rn rate 2 Check if the right value Fig 10 Display control appears on the display Result The setting is completed and the pump runs at the set flow rate Setting the Pressure Switch Off Legend Maximum value D Minimum value e Set maximum pressure to avoid damaging the pump or pump head e Set minimum pressure to avoid running the pump dry Note Ifthe minimum is set to 0 the minimum pump pressure is not monitored Procedure 1 Press both arrow keys simultaneously until the correct display appears Let go of the arrow keys When the cursor flashes use the arrow E 1213 keys again to set the value for the maximum pressure Hold down left arrow key Press right arrow key once Fig 11 Maximum pressure KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 23 Result Legend Maximum value Minimum value Note Procedure KNAUER Operation 5 When the cursor flashes use the arrow E 9 keys again to set the value for the minimum Fig 12 Minimum pressure pressure Hold down left arrow key Press right arrow key once to return to the start displa
22. da bals 35 Position check Valve ss errar aa 35 KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 47 Index Index A Accessories 8 Additives 7 AVV marking 44 C Care 32 CE marking see Declaration of conformity 45 Check valves Cleaning 35 Exchanging 34 Installing 35 Removing 35 Cleaning 32 Connectors on the Rear Side 14 Contact 32 Controlling the pump 21 D Declaration of Conformity 45 Decontamination 9 Device safety 9 Disposal 44 E Electrical connections 18 Eluent 7 Eluent line Connecting the pump head 20 Eluents 7 Flammability 9 Self ignition point 10 Toxicity 10 F Features 6 Flushing the pump 24 Initial Startup 14 Installation 12 Installation location see location 12 Installation Qualification 31 IQ 31 L Labeling pump head 19 LAN Port 16 Router 16 Settings 15 Setup 15 Troubleshooting 17 Line voltage 12 Location 12 M Mains connection 19 Maintenance 32 by user 32 maintenance contract 32 Mandatory signs 11 Manufacturer 43 Modifiers 7 O Operation Arrow keys 21 Operation Qualification 31 OQ 31 P Power supply 10 12 19 Power cable 10 Power strip 10 Pump control 21 Pump head exchanging 33 Installing 34 Labeling 19 removing 33 Pumpensteuerung 21 R Remote terminal strip 18 KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 48 Index S Safety 9 Salts 7 see Power supply 12 Self test 20 Setting the Flow Rat
23. dth x Pump with pressure sensor height 227 8 x 121 x 138 1 mm KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 40 Abbreviations and Terminology Abbreviations and Terminology Here you can find information on the abbreviations and termi nology used in this manual Explanations GLP Good Laboratory Practice quality assurance for laboratories High Pressure Gradient Operating mode of an HPLC system The solvent is mixed on the high pressure side of the pump HPLC High Performance Liquid Chromatography Solvent Mobile phase eluent or carrier for liquid chro matography Remote External control with chromatography software or analog control signals KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 41 Accessories and Spare Parts Accessories and Spare Parts KNAUER Device and Accessories Name ordermumber Accessories kit AZURA FZAO1 Accessories kit P 2 1S P 4 15 User manual English V6870A Device Variations Pump P 4 15 with 10 ml pump head APG20AA stainless steel Pump P 4 15 with 10 ml pump head APG20BB ceramic Pump P 4 15 with 10 ml pump head APG20AC titanium Pump P 4 15 with 50 ml pump head APG20DA stainless steel Pump P 4 15 with 50 ml pump head APG20DC titanium Pump P 2 1S with 10 ml pump head APG90AA stainless steel Pump P 2 15 with 10 ml pump head APG90AC titanium Pump P 2 1S with 10 ml pump head APG90BB ceramic Pump P 2 1S with 50 ml pump
24. e company network 4 Restart all device including the computer Controlling Several Systems Separately in a LAN Devices connected to a LAN communicate through ports which are part of the IP address If more than one HPLC system is con nected to the same LAN and you plan on controlling them sepa rately you can use different ports to avoid interference Therefore the port number for each device must be changed and this same number must be entered into the device configu ration of the chromatography software We recommend to use the same port number for all devices in the same system The port is set to 10001 at the factory You must use the same numbers in the device configuration of the chromatography software as in the device otherwise the connection fails 1 Change the port number of the device 2 Enter the port number in the chromatography software Pump P 2 1S P 4 15 User Manual V6870A version 1 1 17 Initial Startup Result The connection is established Troubleshooting for Connection Problems In case no connection between the computer and the devices can be established go through the following points Check after each point if the problem is solved If you did not manage to locate the problem call the Technical Support 1 Check the status of the LAN connection in the Win dows taskbar fal Connected 5 Connection not established If no connection was established test the following Is the router on
25. e 21 Setting the power consumption 23 Software 21 Local Area Network 29 Solvents 7 Spare Parts 8 Steuerung der Pumpe 21 Switch on 20 T Technical Data 40 Technical data 38 Technical support 12 32 Test intervals 31 Transportation damages 43 W Warning instructions 9 Warning signs 11 Warranty 43 KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 Wissenschaftliche Ger tebau Dr Ing Herbert Knauer GmbH All rights reserved The information in this document is subject to change without prior notice Translation of the original German edition of this manual version 1 1 2013 04 17 Printed in Germany on environmentally friendly paper from sustainable forests AZURA are registered trademarks of Wissenschaftliche Geratebau Dr Ing Herbert Knauer GmbH Bb See up to date manuals online www knauer net downloads HPLC SMB Osmometry Wissenschaftliche Geratebau Phone 49 30 809727 0 Dr Ing Herbert Knauer GmbH Telefax 49 30 8015010 Hegauer Weg 38 E Mail info knauer net 14163 Berlin Germany Internet www knauer net www knauer net Il KNALIER KNAUER 2012 V6870A 0 01 10 12 Koe
26. equipped with a pressure sensor Liquid transport with stable flow rate and high flow accuracy Long service life Pump heads completely made of stainless steel or with tita nium or ceramic inlays Piston backflushing High physical and chemical stability Flexible control with LAN connection RS 232 interface and analog control signals Control with chromatography software A pump in combination with another pump can be used to set up a binary high pressure gradient system Pump P 2 1S P 4 15 User Manual V6870A version 1 1 7 Note Suitable eluents Acetone Acetonitrile Benzene Chloroform Ethyl acetate Ethanol Hexane heptane Isopropanol Carbon dioxide liquid 99 999 CO2 Methanol Phosphate buffer solu tions 0 5 M Toluol Dilute ammonia solu tion Dilute acetic acid 10 50 at 25 C Dilute sodium hydrox ide 1M Water KNAUER Intended Use Eluents Even small quantities of other substances such as additives modifiers or salts can influence the durability of the materials The list of selected solvents was compiled based on research in the pertinent literature and is only a recommendation If there is any doubt contact the technical support of the manufacturer Less suitable eluents Not suitable eluents Dimethyl sulfoxide DMSO Slightly volatile eluents Methylene chloride Tetrahydrofuran THF Dilute phosphoric acid Halogenated hydrocar bons e g Freon Concentrated min
27. er with a hose Connect the inlet to the syringe with a hose Fill up flushing solution with the syringe through the pump head until there are no more air bubbles run ning through the waste bottle Fig 15 Flushing rear piston area 4 Afterwards remove the hoses and connect inlet and outlet with a hose KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 26 Control commands Note Control command ADJ10 ADJ50 CORR10 CORR50 FLOW PRESSURE PMIN10 PMIN50 PMAX10 PMAX50 IMIN10 IMIN50 IMAX10 IMAX50 KNAUER Operation External Control The control commands listed below are considered for the com munication with RS 232 and LAN When entering a parameter you must place a colon or space between command and param eter value e g PMIN10 100 Consider the following specifications for data transfer with RS 232 interface 9600 baud 8 bit 1 stop bit no parity check Range and specifica Description tion RD WR 100 2000 Adjust parameter for 10 ml pump head RD WR 100 2000 Adjust parameter for 50 ml pump head RD WR 0 300 Correction parameter for 10 ml pump head RD WR 0 300 Correction parameter for 50 ml pump head RD WR 0 50000 Writing reading the flow in pl min RD 0 400 Pressure readout in 0 1 MPa RD WR 1 400 Minimum pressure for 10 ml pump head in 0 1 MPa RD WR 150 Minimum
28. eral and organic acids Concentrated bases Eluents containing parti cles Perfluorinated eluents e g Fluorinert FC 75 FC 40 Perfluorinated polyether e g Fomblin Pump P 2 1S P 4 15 User Manual V6870A version 1 1 8 Scope of Delivery Scope of Delivery Note Only use original parts and accessories made by KNAUER or a company authorized by KNAUER Delivery Pump P 2 1S P 4 15 a User manual English and German MEN Power adapter 24 V o Installation Qualification Document E ee AZURA o KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 9 Safety Professional group What must be taken into account Safety The user manual is addressed to persons that have qualification as chemical laboratory technician or comparable vocational training The following knowledge is required Fundamental knowledge of liquid chromatography Knowledge regarding substances that are suitable only to a limited extent for use in liquid chromatography Knowledge regarding the health risks of chemicals If you do not belong to this or a comparable professional group under no circumstances may you perform the work described in this user manual All safety instructions in the user manual The environmental installation and connection specifications in the user manual National and international regulations pertaining to labora tory work Original spare parts tools and eluents made or recom mende
29. everal devices are connected to one power strip always consider the maximum power consumption of each device Power cable Defective power cables are not to be used to connect the device and the mains power Self ignition point Only use eluents that have a self ignition point higher than 150 C under normal ambient conditions Power supply Only connect devices to voltage sources whose voltage equals the device s voltage Toxicity Organic solvents are toxic above a certain concentration Ensure that work areas are always well ventilated Wear pro tective gloves and safety glasses when working on the device Where is use of the Never use the system in potentially explosive atmospheres with device prohibited out appropriate protective equipment For further information contact the Technical Support of KNAUER Opening the device The device may only be opened by the Technical Support of KNAUER Definition of Personal and Material Dam ages Possible dangers related to the device are distinguished in per sonal and material damages in this user manual Type of damage Category Explanations Personal and material DANGER Lethal or very serious injuries can occur damages CAUTION Moderate injuries can occur Device defect can occur KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 11 Symbols and Signs Symbols and Signs CE mark Warning signs Mandatory signs Device fulfills the requirements of the Con
30. g screw on the pressure sensor is turned open Pump does not Check the following options transpomsoiwent Purge the pump head so as to remove the air bubbles Inspect the eluent inlet and filter of the HPLC column and change when blocked Replacing the pump head Clean the check valves Exchange the check valves If the pump head seals are defec tive solvent enters the piston back flushing inform the technical support of KNAUER Pressure or flow rate Check the following options fluctuations Purge the pump head so as to remove the air bubbles Always tighten the inlet screw and outlet screw on the pump head with a torque wrench and 7 5 Nm Clean the check valves Exchange the check valves KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 37 Troubleshooting Pump head leaks Check the following options Inspect the inlet and outlet screw fittings of the pump head Replacing the pump head If the pump head seals are defec tive solvent enters the piston back flushing inform the technical support of KNAUER Flow rate is not cor Check the following options rect Inspect the inlet and outlet screw fittings of the pump head Clean the check valves Exchange the check valves Replacing the pump head Pump without pressure sensor Pay attention to the influence of the pressure on the flow rate will not be compensated Inform the Technical Support of KNAUER KNAUER Pump P 2 1S P 4
31. he rear of the device for air circulation Pump P 2 1S P 4 15 User Manual V6870A version 1 1 13 Note Result Next steps KNAUER Unpacking and Setup Unpacking 1 6 7 8 Setup the delivery so you are able to read the label Using the utility knife cut the adhesive tape Open the delivery Remove the foam insert Take out the accessories kit and the manual Open the accessories kit and take out all accessories In case any parts are missing contact the technical support Grip the device at its side panels and lift it out of the packaging Remove the foam inserts from the device Pull off the anti static bag if necessary Check for damages caused during transportation In case you notice any damage contact the technical support Set up the device in its location Check the scope of delivery Remove the protective foil The device is complete and ready for use Connect the device to power supply to prepare the initial startup Pump P 2 1S P 4 15 User Manual V6870A version 1 1 14 Initial Startup Legend O CE mark Opening of the fan Serial number RS 232 port LAN port Pin header for remote control Power connection bushing Warning 1 Hole for the ground connection Note Process KNAUER Initial Startup Ports on the Rear Side All connectors are located on the rear side of the detector LAN 1 GROUND START IN ERRO
32. leted the self test Result After the device is switched on the display shows Pump and the Firmware version The device performs a self test After all tests have been successfully completed the status of the pump with its current flow rate is displayed The pump is ready for opera tion KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 21 Operation Note Display Legend O Pressure in 0 1 MPa Flow rate in ml min Buttons KNAUER Operation The pump can be operated in two ways Using the buttons at the device Using the software ClarityChrom or ChromGate Operator errors and clogged capillaries can cause high pressure spikes The status display shows the flow rate and also the pressure for pumps with pressure sensor IS 1 2 34 Fig 7 Status display Operating with the Keypad The keypad consists of 3 keys which allow monitoring the device or changing the settings Press both keys simultaneously to P scroll Press any of the arrow keys to set val Arrow keys ues and to change settings Starting and stopping the pump Flushing the pump Fig 9 Start stop key Setting the Flow Rate In case of the pump without pressure sensor the actual required flow rate is dependent on the resulting counter pressure The absolute deviation is dependent on the compressibility and the viscosity of the used solvent and on the pump Therefore it must be determine
33. nnected to the power supply 1 Windows Start gt All Programs gt Accessories Communica tions gt HyperTerminal 2 Connect the pump using a LAN or RS 232 3 Enter EXTCONTR 1 so as to control the flow rateexternally in ANALOG IN 4 Apply control voltage 5 Press the Start Stop button so as to start the pump The star symbol on the display of the pump indicates that the pump is working with an externally controlled flow rate 6 To stop the pump press the start stop key again Pump P 2 1S P 4 15 User Manual V6870A version 1 1 28 Legend O Pressure in 0 1 MPa Flow rate in ml min Note STARTLEVEL Note Legend O Pressure in 0 1 MPa Flow rate in ml min Prerequisite KNAUER Operation J o AA Fig 16 Status display The entry EXTCONTR 0 default setting in the terminal program disables the external control of the flow rate using ANALOG IN Starting with a Short Circut Connection for the short circuit or TTL low for interrupting and continuing the operation of the pump The operation of the pump is dependent on the STARTLEVEL setting STARTLEVEL 1 default setting The pump does not operate during the time of short circuit STARTLEVEL 0 The pump operates during the time of short circuit During the interruption the horizontal arrow A remains in the display because the pump is still in operation status alk AAEL O Fig 17 Status display Enter STA
34. ntacting the Technical Support 32 Technical Support Hotline veria tenseur ne dat e das 32 Maintenance GonthaCts Caca dan nd 8 sd MOE de most Ree ee 32 What maintenance tasks may users perform on the device 32 Replacing the Pump Head 3 3 Ses etd kh Da ARA 33 Removing the Pump Head 2 4 38 34 ADE AT RARE Sales 33 Installing the Pump Head 44 vik sar wah yaw ete doin 34 Exchanging the Check Valves 34 Removing the Check Valves 2 acs anaes ye AAA ARA 35 Cleaning the Check Valves scudo ia s 35 Installing the Check Valves varia ds oe E CHES E ene esas 35 Troubleshooting aaron 36 Error List and SGlUtiONS vila toes Sea dana eek Beeler ek ees 36 Technical Data sieste Dose dt FE ARR ARE Re be es 38 Abbreviations and Terminology 40 Accessories and Spare Parts 41 Device anid ACCESSORIES cag ria e du de eS ae beeen eek eee 41 DEVICE Variati ns Serias 41 Available Pump Heads Si ong thw Rd rele A AAA 42 Legal Information 2555454048 IE 43 Warranty CONQINONS vaa das nan ca 43 Manufactur t ra a is 43 Transportation Damages ii ada 43 DISPOSAL A A A A e da eee 44 Declaration of Conformity 45 Table of Figures 0 ii A AAA ten RS 46 aTe A O A RENA 47 KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 5 Intended Use Intended Use Note Onl
35. pressure for 50 ml pump head in 0 1 MPa RD WR 0 400 Maximum pressure for 10 ml pump head in 0 1 MPa RD WR 0 150 Maximum pressure for 50 ml pump head in 0 1 MPa RD WR 0 100 Minimum motor current for 10 ml pump head RD WR 0 100 Minimum motor current for 50 ml pump head RD WR 0 100 Maximum motor current for 10 ml pump head RD WR 0 100 Maximum motor current for 50 ml pump head Pump P 2 1S P 4 15 User Manual V6870A version 1 1 27 Operation Control command HEADTYPE STARTLEVEL ERRIO STARTMODE EXTCONTR EXTFLOW IMOTOR LOCAL REMOTE ERRORS ON OFF Prerequisite KNAUER Range and specifica Description tion RD WR 10 50 Writing reading the pump head type RD WR 0 1 Sets logical level of the START IN input to start the flow delivery RD WR 0 1 Writing reading the ERROR input output OUT 0 or IN 1 RD WR 0 1 0 Pump pauses after switch on 1 Pump starts with last used flow rate at switch on WR 0 1 1 Allows flow control through analog input 1 V 1 5 ml min Put the instrument in local mode Put the instrument in remote mode O Prevents external flow control Returns 5 last saved errors Start flow Controlling the Flow Rate The control of the flow rate by external control voltage is pre pared with a terminal program e g with the Windows operat ing system program HyperTerminal The description is considered for Windows XP The pump is co
36. r cable is to be used in connection with the external power adapter to connect the device to the power supply Labeling on the Pump Heads The front of the pump head is labeled with the specifications for the maximum pumping capacity 10 ml or 50 ml Pump heads with inlays carry additional material labels Ti for titanium Legend O Labeling on pump head stainless steel without inlays O Labeling on pump head with inlays titanium Fig 5 Labeling on the pump heads KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 20 Initial Startup Connecting the Eluent Line to the Pump Head Note Make sure that the tapered side of the cutting ring is pointed towards the fitting Procedure 1 Push the Teflon hose through fitting and cutting ring Insert the hose end as far as possible into the inlet of the pump head Tighten the fitting by hand O O O Fig 6 Connect the eluent line to the pump head Switching on the Pump CAUTION Defect of the device caused by condensed water Allow device to acclimate for 3 h before it is taken into operation CAUTION Damage to the pump head in case it runs dry Ensure that liquids runs through pump head and piston backflushing CAUTION Procedure 1 Connect the power adapter to the power supply 2 Connect the pump with plug from the external power adapter 3 Switch on the power adapter 4 Wait until the pump has comp
37. u use an USB to COM box the option Allow the computer to turn off ths device to save power in the device manager must be deactivated for all USB hosts Only for Windows 7 For the network adapter the option Allow the computer to turn off ths device to save power in the devicemanager must be deactivated 1 In Windows 7 choose Start gt Control Panel Network and Sharing Center Double click on LAN Connection Click on the button Properties Select Internet Protocol version 4 TCP IPv4 Click on the button Properties Nn BW N Check the settings in the tab General The correct settings for the DHCP client are a Obtain an IP address automatically b Obtain DNS server address automatically 7 Click on the button OK Connecting the Cables A router has several LAN ports and one WAN port that can be used to integrate the LAN into a wide area network WAN e g a company network or the Internet On the other hand the LAN ports serve to set up a network from devices and a computer To avoid interference we recommend to operate the HPLC system separate from the company network You will find patch cables for each device and the router in the accessories kit To connect the router to a WAN an additional patch cable is required which is not supplied within the scope of delivery Fig 4 Cabling system LAN Pump P 2 1S P 4 15 User Manual V6870A version 1 1 16 Prerequisite
38. y The setting is completed If the maximum pressure is exceeded the pump switches off If the minimum pressure is undercut the pump switches off after 30 s The display shows an error message in both cases Setting the Power Consumption The power consumption is dependent on the flow rate and the counter pressure It increases with higher flow rates and stronger counter pressure e Exceeding or undercutting the values for the maximum or minimum power consumption leads to the pump being automatically shutdown e Set the maximum power consumption for the pump without pressure sensor to limit the pump pressure e Set the minimum power consumption so as to avoid a dry run of the pump at highly reduced maximum power con sumption e g if leaking The pump is preset to a standard value for the maximum power consumption KNAUER recommends that with smaller flow rates the standard value for the maximum power consumption should be insignificantly decreased If the minimum is set to 0 the minimum power consumption is not monitored 1 Press both arrow keys simultaneously until the correct display appears 2 Let go of the arrow keys Pump P 2 1S P 4 15 User Manual V6870A version 1 1 24 Result Legend O Flushing symbol O Pressure Flow rate Note Prerequisite Procedure KNAUER Operation When the cursor flashes use the arrow JE OI5IO keys again to set the value for the maximum power
39. y use the device for applications that fall within the range of the intended use Otherwise the protective and safety equip ment of the device could fail Device Overview The HPLC pumps P 4 1S P 2 18 with pump heads can be used as feed pumps or dosing pumps in analytical or preparative applications Pumps transport solvents or dissolved samples through the HPLC system Legend P 2 15 HPLC pump P 4 15 HPLC pump O Display Keypad Pump head Pressure sensor Fig 1 Pump 2 15S with Pump 4 15 with 10 ml pump head 10 ml pump head Location In laboratories the device can be used in the following areas Biochemistry analyses Food analyses Pharmaceutical analyses Environmental analyses Chemical analyses Dosing applications KNAUER Pump P 2 1S P 4 15 User Manual V6870A version 1 1 6 Features Options KNAUER Intended Use Features Analytical pump head with a flow rate range from 0 001 9 999 ml min and a pressure of up to 400 bar Dual piston technology for constant flow rates Setting a limit for minimum and maximum pressure to pro tect the columns and to avoid a dry run of the pump Emergency stop independent from control with chromatog raphy software The pump can be controlled with the keypad in standalone mode or with the chromatography software The pump heads can be easily removed and replaced via four front accessible screws by the user Unlike the pump P 2 15 pump P 4 15S is
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