Proline®Plus pipette
Contents
1. 4 Dispense the liquid by pressing the operating button to the first stop After a short delay press the operating button to the second Stop This action will empty the tip 8 2 Reverse pipetting Fit the tip onto the pipette tip cone 2 Press the operating button all the way to the second stop 3 Place the tip just under the surface of the liquid 2 3 mm and smoothly release the operating button up to the starting position 4 Withdraw the tip from the liquid touching against the edge of the container to remove excess 5 Press the operating button smoothly to the first stop to deliver the desired volume Hold the operating button at the first stop The liquid that remains in the tip should not be included in the delivery 6 Discard the remaining liquid by pressing the operating button to the second stop ch D 8 3 Repetitive reverse technique 1 Follow the reverse technique steps 1 to 5 2 Continue pipetting by repeating steps 3 to 5 as long as needed 3 Finally discard the remaining liquid by N O HT pressing the operating button to the second stop 9 Recommendations for good pipetting Make sure that the tip is firmly attached to the tip cone Hold the pipette vertically when aspirating the liquid and place the tip only a few millimeters into the liquid Always control the operating button slowly and smoothly Pre rinse the tip before aspirating the liquid b2. 50 25 5 100 50 10 200 100 20 1000 500 100 5000 2500 500 10 000 5000 1000 10 20 25 50 100 200 250 500 1000 2000 5000 10 000 100 50 10 300 150 30 gi 100 50 10 300 150 30 Inacc 1 30 2 40 10 0 1 00 1 50 2 50 0 90 1 20 3 00 1 00 1 40 3 00 0 80 1 00 2 00 0 60 0 80 2 00 0 60 0 60 1 00 0 50 0 60 2 00 0 60 1 20 3 00 1 30 0 80 0 60 0 50 0 50 0 50 0 40 0 40 0 30 0 30 0 30 0 30 0 60 1 50 2 50 4 00 0 70 1 00 3 00 0 60 1 00 2 00 1 50 2 50 4 00 0 70 1 00 3 00 0 60 1 00 2 00 Impr 0 80 1 60 6 00 0 60 1 00 1 50 0 40 1 00 2 00 0 30 0 50 1 50 0 15 0 40 1 00 0 15 0 30 0 80 0 20 0 20 0 40 0 20 0 30 0 60 0 20 0 30 0 60 1 20 1 80 0 50 0 30 0 30 0 30 0 20 0 20 0 20 0 20 0 15 0 15 0 20 1 00 2 50 4 00 0 25 0 70 1 50 0 25 0 50 1 00 1 00 2 50 4 00 0 25 0 70 1 50 0 25 0 50 1 00 Sartorius Biohit Liquid Handling Oy Laippatie 1 FI 00880 Helsinki Finland Phone 358 755 951 Ihinfo finland sartorius com www sartorius com Headquarter Sartorius Corporate Administration GmbH Weender Landstrasse 94 108 37075 Goettingen Germany Phone 49 551 308 0 Fax 49 551 308 3289 www sartorius com Copyright by Sartorius Helsinki Finland All rights reserved No part of this publication may be reprinted or translated in
3. the tip cone cylinder and the piston 4 with ethanol 70 isopropanol 60 or mild detergent and soft lint free cloth 5 Clean the interior of the tip ejector collar and the tip cone cylinder with a cotton swab Be careful with the pipettes so that the seal inside the tip cone will not damage 6 Rinse the parts with distilled water if needed and let the parts dry 7 Pipettes 100 ul Put a thin layer of grease on the piston 4 Pipettes 250 500 and 1000 ul Put a thin layer of grease around the piston seal 4 Pipettes 2000 5000 and 10 mi Put a thin layer of grease on the piston seal 4 and the interior of the tip cone cylinder 3 NOTE Avoid excess grease Use only the grease provided with the pipette NOTE Before reassembling check that no lint or particles are on the surface of the piston Reassembling 1 Pipettes Carefully place the tip cone 3 on the piston and attach it by screwing the tip cone holder Connecting 2 clockwise B collar Pipettes 2000 5000 and 10 ml e Carefully place the tip cone cylinder 3 x on the piston and screw clockwise Make sure that the tip cone cylinder is properly tightened Avoid over tightening 2 Attach the tip ejector collar 1 by screwing it clockwise 3 Place the new tip cone filter on its place 4 Press the operating button several times to ensure that the grease has spread evenly 5 Check the performance of the pipette 121 C NOTE It i
4. through the display The volume setting is carried out with the following steps B gt C l I 1 Set the volume by rotating the operating button clockwise to decrease the volume and counterclockwise to increase NOTE Do not rotate the operating button outside the volume range of the pipette 7 2 Sealing and ejecting tips To ensure the maximum accuracy and precision Sartorius tips are recommended to be used with Proline Plus pipettes Before fitting a tip ensure that the pipette tip cone is clean Press the tip on the tip cone of the pipette Eject the tip by pressing the tip ejector with your thumb Make sure that the tip is disposed into a suitable waste container 7 3 Protective tip cone filters Tip ejector The tip cone design of the Sartorius pipettes gt 10 ul allows the use of Safe Cone Filters in the tip cones These removable filters prevent liquids and aerosols from entering the pipette and thus protects the pipette from contamination and damage The Safe Cone Filters are available as Standard or Plus versions It is recommended to use the Standard filter for general applications and the Plus filter for more demanding applications such as cell culture bacterial and virological work and molecular biology Filters need to be exchanged regularly The interval for filter exchanging is application dependant but the recommendation is to change the filter daily after 50 250 pipetti
5. acceptable maximum permissible errors established by the user ISO 8655 1 11 1 Testing the performance Performance testing should take place in a draught free room at 15 30 C constant to 0 5 C and humidity above 50 The pipette tips and the test water should have stood in the test room a sufficient time at least 2 hours to reach equilibrium with the room conditions Use distilled or deionised water grade 3 ISO 3696 Use an analytical balance with a readability of 0 01 mgs ISO 8655 6 Weighing 1 Adjust the desired test volume Vs 2 Carefully fit the tip onto the tip cone 3 Fill the tip with test water and expel to waste five times to reach a humidity equilibrium in the dead air volume 4 Replace the tip Pre wet the tip by filling it once with test water and expel to waste 5 Aspirate the test water immersing the tip only 2 3 mm below the surface of the water Keep the pipette vertical 10 Withdraw the pipette vertically and touch the tip against the inside wall of the test water container Pipette the water into the weighing vessel touching the tip against the inside wall of the vessel just above the liquid surface at an angle of 30 to 45 Withdraw the pipette by drawing the tip 8 10 mm along the inner wall of the weighing vessel Read the weight in mgs mi Repeat the test cycle until 10 measurements have been recorded Convert the recorded masses m to volumes V V mZ Z c
6. 0 1 0031 1 0031 1 0031 21 5 1 0031 1 0032 1 0032 1 0032 22 0 1 0032 1 0033 1 0033 1 0033 22 5 1 0033 1 0034 1 0034 1 0034 23 0 1 0034 1 0035 1 0035 1 0036 23 5 1 0036 1 0036 1 0036 1 0037 NOTE This method is based on ISO 8655 11 2 Recalibration The calibration of your Proline Plus pipette has been factory checked by using forward pipetting technique and certified at 22 C using grade 3 distilled water according to ISO 3696 The calibration is based on ISO 8655 6 Gravimetric test method for volumetric instruments The pipette s specifications are guaranteed only with genuine Sartorius tips If you find the pipette to be inaccurate after performance testing please follow the instructions below 1 Remove the lid of the calibration nut located on the back of the handle with the aid of the calibration tool 2 Place the hexagonal head of the calibration tool into the hole of the calibration nut 3 Turn the adjustment lock counterclockwise to decrease and clockwise to increase the volume 4 Repeat testing the performance procedure Chapter 11 1 Continue until the results are correct NOTE Recalibration is recommended if reverse pipetting technique is used NOTE Sartorius offers accredited calibration service Please contact your local Sartorius representative for further information 12 e T H Wd d ee 12 Trouble shooting Symptom Droplets left inside the tip Leakage or pipett
7. 1 C 252F 20 min 1 bar 15 p s i All Single Tray Racks and clean Refill Packs are certified RNase DNase and endotoxin free For more information please go to www sartorius com or contact your local Sartorius support 721014 721008 721008 721008 721007 721006 721006 721006 721005 721005 721005 721008 721007 721008 721007 721018 721018 721018 721017 721016 721016 721016 721015 721015 721015 721018 721017 721018 721017 4 Unpacking The Proline Plus pipette package contains the following items 1 Pipette 2 Tip 3 Calibration tool also tube opener 4 Safe Cone filters pipettes gt 10 ul and forceps 5 Grease 6 Instruction manual 7 Performance certification in accordance with ISO 8655 6 Please check that all items are included and that no damage has occurred during shipment 5 Pipette holder and carousel stand For convenience and safety always keep the pipette vertically on its own holder carousel or linear stand when not in use Cat No Product 725600 Carousel Stand for 6 pipettes 725610 Pipette Holder for 1 pipette 725620 Linear Stand 6 Calibration tool also tube opener The calibration tool is designed for the following purposes 1 Tool for recalibration A 2 Opening tool for the lid of the calibration nut B 3 Opening tool for the tubes C 7 Operating the pipette 71 Setting the volume The volume of the pipette is clearly shown
8. 10 728030 Yellow 1 2 20 0 02 200 721014 728040 Yellow 1 5 50 0 1 200 721008 721018 728050 Yellow 1 10 100 0 1 200 350 721008 721018 728060 Yellow 1 20 200 0 2 200 350 721007 721017 728070 Blue 1 100 1000 1 1000 721006 721016 728080 Green 1 500 5000 10 5000 721005 721015 728090 Red 1 1 10 ml 20 10 000 721005 721015 728515 Grey 1 5 10 728520 Grey 1 10 10 728530 Yellow 1 20 200 728535 Yellow 1 25 200 728545 Yellow 1 50 200 728550 Yellow 1 100 200 350 728560 Yellow 1 200 200 350 728565 Blue 1 250 1000 728567 Blue 1 500 1000 728570 Blue 1 1000 1000 728575 Green 1 2000 5000 728580 Green 1 5000 5000 728590 Red 1 10 ml 10 000 728120 Grey 8 0 5 10 0 01 10 728130 Yellow 8 10 100 0 1 200 350 728140 Orange 8 30 300 0 2 350 728220 Grey 12 0 5 10 0 01 10 728230 Yellow 12 10 100 0 1 200 350 728240 Orange 12 30 300 0 2 350 3 Sartorius pipette tips Sartorius pipette tips are recommended for use with Proline Plus pipettes The use of fully compatible tips will ensure the maximal pipetting accuracy and precision and thereby guarantee the performance specifications given for the pipette Sartorius tips are made of pure virgin polypropylene and manufactured in protected clean room conditions Sartorius offers a full range of standard Optifit and SafetySpace Filter tips Sartorius non filter tips are available in trays bulk packages and space saving refill systems Sartorius non filtered tips and trays are autoclavable at 12
9. 184 Sartorius Proline Plus pipette H User Manual Bedienungsanleitung Mode d emploi Manual Usuario Instruzioni d impiego VIHCTPYKLUA nonb3oBaTena SH Sartorius Proline Plus pipette BJ DUAN UG LR Rene 1 Bedienungsanleltung EE 15 Mode d emploi ssssssssssssssennessnssnrnnsrrnsnnesnnssnrnnnnnnnnenns 31 Manual EE FO EE 47 Instruzioni IMPIEYO errenenrrvrvenerervererererveneservenenns 63 VIHCTPYKLIUA NONb3OBATENA 79 EE 97 Huom Suomenkielinen k ytt ohje ladattavissa osoitteesta www sartorius com Obs Manual p svenska kan du ladda ner p adressen www sartorius com Contents kp TT E 2 2 EIER IDIOM eerste 2 3 Sartorius pipette pS aesir aasaran anae akeas i 3 A Aere e EE 4 5 Pipette holder and carousel SN nn 4 D teg e ak e ME 4 T ENE 4 Ti NN 4 12 Send ejecting TOS avden 5 13 Protective tip cone ES s cccacssssassnsnecscsesactesesasesnsniacxeuasasiassansves 5 o PN Nr 5 81 NN 6 3 2 Reverse pipe le KE 6 2 Repetitive reverse FO NGU reresik ien 6 9 Recommendations for good pipetting 7 ET CSI NC is ea EEE 7 10 1 Daily cleaning of the outer surface of the pipette 8 10 2 Cleaning and the lower part of the pipette eee 8 10 3 Sterilization of th pipette eien 9 11 Testing the performance and recalibration em messeeseeseesvesververversenn 10 i Testing the PT NE me 10 NES He 12 Keele te nn nca 13 13 NNN 14 14 Performance spec
10. CE OR NEGLIGENCE OF REGULAR MAINTENANCE AND SERVICE ACCIDENTAL DAMAGE INCORRECT STORAGE OR USE OF THE PRODUCTS FOR OPERATIONS OUTSIDE THEIR SPECIFIED LIMITATIONS OUTSIDE THEIR SPECIFICATIONS CONTRARY TO THE INSTRUCTIONS GIVEN IN THIS MANUAL OR WITH OTHER THAN THE MANUFACTURER S ORIGINAL TIPS Each Proline Plus pipette is tested before Shipping by the manufacturer The Sartorius Quality Assurance Procedure guarantees that the Proline Plus pipette you have purchased is ready for use 14 Performance Specifications The manufacturer s specifications p 97 are guaranteed only when the manufacturer s original tips are used The manufacturer s Specifications should be used as guidelines when establishing your own performance specifications in accordance with ISO 8655 Specifications Proline Plus single 8 and 12 channel pipettes Cat No 728010 728020 728030 728040 728050 728060 728070 728080 728090 128515 728520 728530 728535 728545 728550 728560 728565 728567 728570 728575 728580 728590 728120 728130 728140 728220 728230 728240 Channels wei ei dd sch CO Volume Range u 0 1 3 0 5 10 2 20 5 50 10 100 20 200 100 1000 500 5000 1 10 ml 10 20 25 50 100 200 250 500 1000 2000 5000 10 ml 0 5 10 10 100 30 300 0 5 10 10 100 30 300 Test Volume ul 3 1 5 0 3 10 5 1 20 10 2
11. any form or by any means without the prior written permission of Sartorius The status of the information specifications and illustrations in this manual is indicated by the date given aside Sartorius reserves the right to make changes to the technology features specifications and design of the equipment without notice All trademarks are Sartorius property unless otherwise stated Patents granted or pending 4002250 e 03 2013 e Sartorius Biohit Liquid Handling Oy
12. ed volume too small Pipette out of established specifications Operating button jammed or moves erratically Tip ejector jammed or moves erratically Possible cause Unsuitable tip Non uniform wetting of the plastic Tip incorrectly attached Unsuitable tip Foreign particles between tip and cone Tip cone holder incorrectly tightened Pipette damaged Incorrect operation Unsuitable tip Calibration altered Liquid has penetrated tip cone and dried Safe Cone Filter has been contaminated Insufficient amount of grease on a piston and seal Tip ejector collar has been contaminated Solution Use original Sartorius tips Attach new tip Attach firmly Use original Sartorius tips Clean the tip cone attach new tip Tighten the tip cone holder Return to your Sartorius service representative for servicing Follow instructions Use original Sartorius tips Reclibrate Clean and grease the piston seal Clean the tip cone Change filter Grease accordingly Remove and clean ejector collar and tip cone 13 Warranty information The Proline Plus pipettes are covered by warranty for 2 years against defects in materials and workmanship Should your pipette fail to function at any time please contact your local Sartorius representative ANY WARRANTY WILL HOWEVER BE DEEMED AS VOID IF FAULT IS FOUND TO HAVE BEEN CAUSED BY MALTREATMENT MISUSE UNAUTHORIZED MAINTENANCE OF SERVI
13. ifications ina ann 14 1 Intended use Proline Plus pipette is intended to be used in liquid handling applications for dispensing liquids in total volume range from 0 1 ul to 10 ml It is recommended to use Optifit tips or SafetySpace Filter tips to ensure the best compatibility and performance with Sartorius pipettes This liquid handling device is designed and manufactured to be used as general purpose laboratory equipment Before operating it is recommended to read the user manual which contains useful information e g for good pipetting practice 2 Product description Your new Proline Plus is an autoclavable air displacement pipette The attractive and ergonomical design of the Proline Plus pipette together with its low pipetting forces reduce the risk of repetitive strain injuries RSI The pipette is designed for both right and left handed use NOTE It is known that prolonged pipetting can cause RSI The manufacturer is not responsible for RSI or any related diseases caused by prolonged pipetting The special tip cone design of the pipette offers the possibility to use replaceable Safe Cone Filters to help prevent the contamination and damage of the pipette The pipette uses disposable tips Proline Plus single and multichannel pipettes Cat No Colour Channels Volume Increment Tip __dafe Cone Filters Filters Code Range l ful ful Standard Plus 728010 Grey 1 0 1 3 0 002 10 728020 Grey 1 0 5 10 0 01
14. ng NN cycles and always in case of over aspiration See A Chapter 2 for ordering information The Filter VA Foreceps should be used to avoid touching the soiled filters by hand See Chapter 2 for ordering information Make sure that the filter is disposed into a suitable waste container Clean the tip cone if needed and put the new filter in place H Pipetting techniques Two basic pipetting techniques forward and reverse pipetting are associated with Proline Plus pipettes Forward pipetting is the most common used pipetting technique The technique employs the blow out function ensuring complete delivery of the liquid Reverse pipetting is recommended for highly viscous biological or foaming liquids or very small volumes of liquid A selected volume plus an excess is aspirated into the tip The delivery is done without blow out and thus the excess volume remains in the tip The reverse technique also facilitates the repeated delivery of the same volume If reverse pipetting technique is used the pipette might need recalibration 1 Forward pipetting 8 1 Fit the tip onto the pipette tip cone 2 Press the operating button to the first stop 3 Place the tip just under the surface of the liquid 2 3 mm and smoothly release the operating button up to the starting position Wait one second Carefully withdraw the tip from the liquid touching against the edge of the container to remove excess from the outside of the tip
15. orrection factor Table 1 Calculate the mean volume V delivered V SV 10 For conformity evaluation calculate the systematic error e of the measurement inpul es V V V selected test volume or in es 100 IN VJ Vg For conformity evaluation calculate the random error of the measurement as standard deviation s SIN V 2 n 1 n number of measurement 10 or as coefficient of variation CV 100s V Compare the systematic error inaccuracy and random error imprecision with the values in the performance specifications p 97 or the specifications of your own laboratory If the results fall within the specifications the pipette is ready for use Otherwise check both systematic and random errors and when necessary proceed to the recalibration procedure Chapter 11 2 NOTE Systemic error inaccuracy is the difference between the dispensed volume and the selected test volume Random error imprecision is the scatter of the dispensed volumes around the mean of the dispensed volume ISO 8655 1 NOTE Sartorius specifications are achieved in strictly controlled conditions ISO 8655 6 The user should establish own specifications based on the field of use and the accuracy requirements placed on the pipette ISO8655 1 Table 1 Z values ul mg Temp C Air Pressure kPa 95 100 101 3 105 20 0 1 0028 1 0028 1 0029 1 0029 20 5 1 0029 1 0029 1 0030 1 0030 21 0 1 003
16. s always necessary to check the performance of the pipette after in house service or maintenance 10 3 Sterilization of the pipette The entire Proline Plus pipette can be sterilized by steam autoclaving at 121 C 252 F 1 atm for 20 minutes Remove the tip cone filter if fitted Chapter 7 3 The single channel pipettes can be autoclaved without special preparations Unscrew the lower part of the multichannel pipettes by holding the connecting collar and turning the lower part 360 counterclockwise Put the pipette into the sterilization bag and place it into the autoclave After autoclaving the pipette must be cooled down and left to dry overnight before use Screw the lower part of the multichannel pipettes by holding the connecting collar and turning the lower part 360 clockwise Make sure that the lower part is properly tightened It is recommended to check the performance of the pipette after every autoclaving It is also recommended to grease the piston seal of the pipette after every 10 autoclaving 11 Testing the performance and recalibration It is recommended to check the performance of your Proline Plus pipettes regularly e g every 3 months and always after in house maintenance However the user should establish a regular testing routine for their pipettes with regard to accuracy requirements of the application frequency of use number of operators using the pipette nature of the liquid dispensed and the
17. the tip cone filters may prolong the service interval Change the filters regularly NOTE It is recommended to always use gloves when cleaning the pipette 10 1 Daily cleaning of the outer surface of the pipette Your Proline Plus pipette should be checked every day for cleanliness To clean and decontaminate the outer surface of your pipette use ethanol 70 isopropanol 60 or a mild detergent and a soft lint free cloth Gently clean the surface of the pipette with moistened cloth and wipe dry Pay special attention to the tip cone Change the tip cone filter if needed Chapter 7 3 10 2 Cleaning and the lower part of the pipette If your pipette is in daily use it is recommended to clean decontaminate and grease the pipette every three months It is recommended to send the multichannel pipettes to your local Sartorius service representative for cleaning and greasing To clean and decontaminate the lower parts of the single channel pipette follow these steps Disassembling and cleaning see the pictures under the cover page 1 Eject the tip cone filter if fitted Chapter 7 3 2 Unscrew the tip ejector collar 1 counterclockwise and remove It 3 Pipettes lt 2000 ul Unscrew the tip cone holder 2 counterclockwise and carefully remove it with the tip cone 3 Pipettes 2000 5000 10 ml Unscrew the tip cone cylinder 3 counterclockwise and remove it 4 Clean the tip ejector collar the tip cone holder
18. y filling and emptying the tip for three to five times This is important especially when pipetting liquids with a viscosity and density greater than water or liquids with high vapor pressure e g ethanol Check that the pipette tip and liquid are at the same temperature When pipetting liquids with temperatures different to the ambient temperature change the tip after each pipetting Do not pre rinse the tip To avoid contamination do not rest the pipette on its side especially with the tip attached Change the tip cone filter regularly recommendation after 50 250 pipetting cycles Never strike the tip cone against the tip tray when mounting the tip as this can damage the pipette Avoid rough handling and do not drop the pipette Avoid exposing the unit to extreme temperature changes humidity and dust operating temperature from 15 C to 40 C 10 Maintenance Proline Plus pipettes have been designed for easy in house service If the pipette is used daily it is recommended to clean decontaminate and check the performance of the pipette every three months Sartorius also provides complete repair and recalibration service for your pipette including a service report and performance certificate NOTE Please make sure that the pipette has been decontaminated before you send it to us or your local representative Please advise if any hazardous material has been used with your pipette NOTE The use of
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