Instruction Manual
Contents
1. SC attter M2 is the transmission signal and M5 is the scatter signal The cursor will select either M2 or M5 Display shows M2 TR M5 SC Lamp Slope Bias Explanation Stroke volume Cleaning Water Through cell Rinse pipette f e 4 05 Volt f e 9 15 Volt Position to view adjustments to lamp voltage by potentiometer on front of the instrument Slope for displaying HD value is approximate 1 000 will be showed as 1000 normally in the range of 0 800 1 200 Must be set after calibration The Bias of the displayed HD value it is a constant value for calibration and to be set after calibration When a measurements are linear in a certain measuring range it can occur that a constant difference appears in that range as a result of incorrect calibration This difference is corrected by a slope and bias adjustment Technical value number of pulses per stroke of the pipette Must be 2400 and is corresponding with 10 ml of pipette volume Don t change If changed the pipette can get stuck in lower position Number of ml decon adjustable for optimum cleaning default is 10 Number of ml water adjustable for optimum cleaning default is 20 Cleaning cycles passing through cell default 3 Number of times rinsing the pipette default 1 e LACTOTRONIC Instruction Manual Globulyser Rinse cell Measurements ml water to add Tubing volume Mixing Through cell Explanation2. 300 up to 500 which is closely related to the mean fat globule size nm as measured by a particle size analyser Waste beaker Glass beaker 100 ml to collect cleaning and rinsing fluids Sample beaker Glass beaker 100 ml with magnetical stirrer ca 40 ml of prepared sample material WTR Transmission value of water Water TRansmission WSC Scatter value of water Water SCatter E1 E2 E3 Sample transmission values in different stages of dilution Extinction 3 References 3 1 Globulyser software manual Main Menu when the Globulyser is started the following menu will appear F1 CLEAN F2 ANALYSE F3 MANUAL F4 SERVICE Select F1 CLEAN eee eee LACTOTRONIC eee Instruction Manual Globulyser Following screen will appear CLEAN F1 MENU F2 WASTE BEAKER SET Instrument asks for waste beaker and F2 will start cleaning procedure F1 returns to main menu Select F2 ANALYSE If there was not recently done a zero measurement the instrument will first perform a zero measurement with water before measuring the HD value Following screen will appear ANALYSE F1 MENU F2 WASTE BEAKER SET Instrument asks for waste beaker because of the zero measurement before sample analysis After zero measurement the instrument asks for sample beaker Select F3 MANUAL Following screen will appear MANUAL F1 MENU F3 SAMPLE BEAKER SET Instrument asks for sample beak
3. LACTOTRONIC Globulyser Instruction Manual Company Lactotronic bv De Kiel 26 9206 BG Drachten The Netherlands Tel 31 512 546022 Fax 31 512 546053 E mail info lactotronic com Version January 1th 2010 LACTOTRONIC Instruction Manual Globulyser Table of contents 1 Scope and field of application ccc ee ceeeeeeeeeeeeeeeeeeeeecneeeeeeeeeeeeeeeeseaees 3 INNO eege 3 E Ee 3 3 1 Globulyser software manual EEN 3 3 2 Service leegent 5 E DEE due a E O E 6 3 4 STAR printer users manual 8 e dE 9 5 Apparatus Glassware and Dluente e 9 6 Work E ee 10 6 1 Instrument set up DEE 10 6 2 Sample PUSAN AOI cea cccncdccccdaa cence cner eege NEE 10 6 3 Sample Analyses eege 10 6 4 Instrument performance control chat 11 7 PGsoftwar Glob lyser2PG i rcs ce sinsa eecaee netics 11 LACTOTRONIC Instruction Manual Globulyser 1 Scope and field of application This instruction describes the procedure to determine the Homogenization Degree by the use of a Lactotronic Globulyser The method is applicable to milk and milk products which are sterilized and non sterilized Measurement for fat content is up to 10 The Globulyser cannot be applied to products containing colorants or non dissolvable particles others than fat globules Examples are cocoa fibers and fruit parts 2 Definitions Homo Degree HD Dimensionless number that indicates the homogenization degree of milk products HD value Value from
4. Wanted extinction Number of times pumping clean water through cell standard is 1 The number of pulses correlated with ml via stroke volume used for the first calculations of the exact dilution of the sample Is value is used to calculate the final dilution to get the desired transmission value for the measurement default 375 425 Number of pulses correlated with the volume of the tubing Influences the dilution of the milk product during transmission measurement Factory setting 700 Number of turns times 5 of the magnet stirrer Number of passes of the diluted sample through the measurement cell standard is three Through cell is the number of passes of the pre diluted and prepared samples through the measurement cell prior to the actual measurement This is to avoid carry over contamination and to obtain a homogeneous sample in the cell with extinction of 400 the maximum scatter value will be achieved The instrument dilutes automatically until the extinction is around 400 Min wanted extinction Adjustable from 375 to 400 default 375 Max wanted extinction Adjustable from 400 to 425 default 425 Max allowed extinction When value exceeds the limit instrument gives a Trans span warning Electronic gain value span to render the minimum and maximum transmission value to the maximum electronics span Explanation This setting has the same effect as a slope change To obtain an optimum s
5. as The result is printed out and the system is automatically rinsed 5 Apparatus Glassware and Diluents Glassware and other chemicals and other devices for performing homogenization measurements 1 Globulyser 2 Serial printer STAR Dot Matrix 200F or serial computer cable with Globulyser2PC software 3 Adjustable pipette 1 5ml or similar 4 Erlenmeyer flasks 100ml 5 Glass beakers 100ml 6 Teflon coated magnetic stirrer fleas 15mm 7 Glass flasks with screw caps of 250 ml and 1 I 8 Demineralized water 9 Cleaning Fluid Decon 4 Bring 10 ml of Superdecontamine 33 N V Intersciences S A Brussels into a flask 250 ml Fill up to 250 ml with demi water and close it Swirl gently to avoid inclusion of air 10 A bottle of Tween EDTA solution LACTOTRONIC Instruction Manual Globulyser Be sure that these solutions are absolutely clear and do not contain any particles 6 Work Instruction 6 1 Instrument set up Connect the Globulyser with the printer or the computer Plug in the power plugs of the Globulyser and the printer Load the printer with the ribbon cartridge and paper Check tubing and place the Water and the Decon tube in the required flasks with corresponding diluents Switch on the Globulyser Allow it to stabilize for at least 15 minutes Switch on the printer and put it in ON LINE mode green pilot lamp is lightened Place a beaker with a stirrer on the i
6. er F3 and will start measurement without zero measurement Select F4 SERVICE Following screen will appear F1 MENU F2 PARAM You can return to the main menu by selecting F1 eee eee LACTOTRONIC eee Instruction Manual Globulyser 3 2 Service Menu Following screen will appear F1 MENU F2 PARAM Select F2 PARAM Following screen will appear F1 MENU F2 PASSW F3 CLOCK F4 PRINT F1 back to main menu F2 password protected entry of parameters menu F3 adjust clock time date F4 print instrument settings If F2 PASSW is selected following screen will appear F1 RETURN F2 LOGIN F3 PASSW F4 LOGOUT F1 return to main menu F2 login to parameters menu F3 to change the current password F4 logout to return to main menu If F2 login is selected instrument will ask for operator password To enter password press NUM key and cursor with correct number Factory set password is 6666 A 6 When password is accepted instrument will go into level 1 Select F1 Now it is possible to adjust the following instrument settings Use the arrow keys amp W lt gt to step through the instrument settings eee eee LACTOTRONIC Instruction Manual Globulyser 3 3 Settings Date Time Serial no Day month year hour minutes seconds unique serial number of instrument Don t change Det M2 M5 TR ansmission and
7. etting for this a 40 dilution should produce a value of approximately 400 in transmission in the manual mode The level is electronically stipulated in the form of a number of pulses per second more pulses result in a higher value and vice versa The signal remains constant e LACTOTRONIC Instruction Manual Globulyser Scat span Scat offset Rinse cell Rinse time Ref min trans Ref max trans Ref min scat Ref max scat Optical checks Electronic gain value span to render the minimum and maximum transmission value to the maximum electronics span Explanation The principle of scat span is identical to trans span however now the scatter value is adjusted Intercept bias constant value added Electronic tuning is to adjust the electrical signal in such a way that it equals the real values Explanation This is an Intercept bias that is applied to the whole measuring range It increases or decreases the measuring results homogenization degree Number of times pumps water through cell after a measurement standard is 4 Time in which a new measurement can be performed without first e new zero is done Default 10 minutes Safety level Safety level Safety level Safety level reserved for future use You can return to the main menu by selecting F1 3 4 STAR printer user s manual See the STAR printer user s manual Short description Power off on both the printer and the Globuly
8. nstructions on the display 6 4 Instrument performance control chart Although the instrument performs correctly it is necessary to gain experience about operation performance over a long period As acontrol sample we suggest to use the sterilized milk which is normally used for calibration Essential is to store these cans at room temperature or lower do not freeze Turn them weekly to avoid creaming which makes them useless for this purpose Record every day the cleaning session date WTR WSC Record on at least weekly the results of e The Cleaning sessions e Record date WTR and WSC e Analyzing results of the control sample in duplicate e Record date WTR WSC E1 E2 E3 and HD Always record every change in performance and every change of settings 7 PC software Globulyser2PC See appendix
9. nstrument tray Perform cleaning sessions until the water scatter value WSC is constant Make a print out of the instrument settings at arrival and file this 6 2 Sample preparation Mix the contents just before analysis turn the well closed container some times 180 lengthwise without shaking to prevent enclosure of air Bring with the adjustable pipette the required amount of milk in an Erlenmeyer flask The amount of sample to be used is depending on the fat content of the sample For the correct amount see the values given in table 1 Add 100 ml of demineralized water and swirl gently Add 2 5 ml of Tween EDTA solution swirl again and wait for 5 minutes to let the solution clarify the sample 6 3 Sample Analyses Switch on the Globulyser and allow it to stabilize for at least 15 minutes LACTOTRONIC Instruction Manual Globulyser e Switch on the printer put it in ON LINE mode or start the computer and the program Globulyser2PC e Perform a Cleaning Session F1 e Continue if the measured water scatter value WSC corresponds with the latest measured WSC value within the preset units else start or repeat the cleaning procedure e Offer ca 40 ml of prepared sample 6 2 into a beaker with stirrer and Analyse F2 e Analyse more samples if available within the preset time Rinse Time or end the analysing session by performing a Cleaning Session F1 e Switch off according the i
10. ser Connect the printer with the RS232 9 to 25 pin interface cable Connection on the bottom of the printer and on the back of the Globulyser The RS232 settings are 9600 Baud 8 data bits NO parity Switch on the printer on off switch on the back of the printer Green power LED will light LACTOTRONIC Instruction Manual Globulyser Be sure the ON LINE LED lit continuously If off the printer is off line if flashing an error occurred please refer to STAR printer user s manual Switch on the Globulyser When an error occurs replacing ribbon cartridge or loading a new roll of paper please refer to the STAR printer user s manual 4 Principle A focused light beam is led through a measurement cell with the sample in it An amount of light is scattered by particles inside the sample If there are only fat globules in the sample the amount of scattered light is directly related by the mean fat globule size The amount of scattering is not depending on the amount of fat present in the sample The sample is diluted with water and clarified by use of a chemical solution About 40 ml of this solution is offered to the Globulyser The Globulyser dilutes the clarified fluid until a pre defined transmission is obtained After that the intensity of the scattered light is measured under a fixed angle From this intensity the Homogenization Degree HD is calculated and adjusted according to the calibration with a slope and bi
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