Zenyth 200 Microplate Reader and Spectrophotometer User`s Manual
Contents
1. WARNING Handle the old deuterium lamp with caution The lamp may be hot 6 Remove the deuterium lamp and discard CAUTION Do not touch the glass of the new lamp Fingerprints on the glass may shorten the life of the lamp 7 Taking care not to touch the glass bulb with bare hands align the notch in the base of the lamp with the guide on the lamp block Figure 3 4 and install the new deuterium lamp 8 Tighten the two flathead screws to secure the new deuterium lamp to the lamp block Figure 3 4 9 Reconnect the lamp power cable making sure the connector key is latched Note The lamp power cable connector is keyed which prevents it from being connected incorrectly 10 Close the lamp housing cover 11 Tighten the two Allen screws to secure the lamp housing cover in place 12 Reconnect the power cable and turn on power to the instrument 13 Calibrate the brightness of the new lamp refer to Section 4 2 5 1 Calibrating Lamp Brightness Zenyth 200 User s Manual v 1 0 August 2010 20 blochrom 3 5 Replacing the Halogen Lamp The Zenyth 200 is equipped with a halogen lamp which is used in measurements made at wavelengths between 326 and 1000 nm Note The ADAP Software and Zenyth 200st standalone software report a Lamp defect when the lamp needs to be changed Replacing the halogen lamp requires a 2 5 mm allen wrench or hex screwdriver ballpoint type recommended which is provided with the instrument Note2. be shipped in the future CAUTION Warranty claims are void if transport damages are caused by improper packing Zenyth 200 User s Manual v 1 0 August 2010 10 blochrom TO UNPACK THE ZENYTH 200RT 1 Check the box for any visible damage during transportation In case of damage inform the supplier immediately and keep the damaged packaging 2 Open the box lid and remove the accessories box 3 Carefully lift the Zenyth 200rt by the molded foam packaging encasing it 4 Remove the molded foam packaging from the instrument and place the packaging back in the shipping box 5 Remove the plastic surrounding the Zenyth 200rt and discard 2 2 2 Setting Up and Operating the Zenyth 200rt TO SET UP THE ZENYTH 200RT 1 Place the instrument external computer and peripherals on a dry flat work area with sufficient space for all of the equipment and the required cables 2 Install the ADAP Software on the external computer Refer to Chapter 1 of the ADAP Software Operating Manual for more information 3 Connect one end of the 9 pin serial cable to the serial port on the computer Note Only use the original serial cable supplied with the Zenyth 200rt Other serial cables with identical connectors may not establish communication between the instrument and computer CAUTION Turn off main power to the Zenyth 200rt before connecting it to the external computer 4 Connect the other end of the 9 pin serial cable to the serial
3. by any person including dealers and representatives of the Company which are consistent or in conflict with the terms of this warranty shall not be binding upon the Company unless reduced in writing and approved by an expressly authorized officer of the Company g Parts replaced during the warranty period are warranted to the end of the instrument warranty Note Performance characteristics and specifications are only warranted when Biochrom Ltd replacement parts are used In the event that you need to return your instrument to us for repair or to use our in house recalibration service the following are instructions which should be carried out in full If your instrument or column is still within its warranty period please contact technical support for advice before following this returns process Please quote the serial number and model of your instrument in all correspondence If your instrument or column is out of it s warranty period you must provide a purchase order number for the repair on your returns form If you need to know that cost of repair recalibration please send an e mail to support biochrom co uk Zenyth 200 User s Manual v 1 0 August 2010 5 olochrom 1 SAFETY INFORMATION All Warnings and Cautions in this document include an exclamation point a lightning bolt or a light burst symbol framed within a triangle Please pay special attention to the specific safety information associated with these sym
4. power is on e WARNING Never view the lamp directly without approved eyewear Protect skin from exposure to the light emitted by the lamp The deuterium lamp emits UV radiation e WARNING Turn off the instrument and allow a minimum of 30 minutes for the lamp to cool before servicing During operation the deuterium lamp reaches a temperature of approximately 250 C 482 F e WARNING Handle the old deuterium lamp with caution The old lamp may be hot e CAUTION Do not touch the glass of the new lamp Fingerprints on the glass may shorten the life of the lamp e WARNING Turn off the instrument and allow a minimum of 30 minutes for the lamp to cool before servicing During operation the halogen lamp reaches a temperature of approximately 300 C 572 F e CAUTION Do not loosen or attempt to remove the 4 screws on top of the lamp block assembly Doing so will result in a misalignment of the optical path e WARNING Handle the old halogen lamp with caution The old lamp may be hot Zenyth 200 User s Manual v 1 0 August 2010 7 blochrom e CAUTION Do not touch the glass of the new halogen lamp Fingerprintson the glass may shorten the life of the lamp e WARNING Turn off and unplug power to the instrument before changing fuses Failure to do so can cause electrical shock or equipment damage e WARNING Only Biochrom trained service engineers may perform service procedures on the instrument 1 2 CHEMICAL AND BIOLOGI
5. the Zenyth 200 before connecting it to the external computer e CAUTION Warranty claims are void if transport damages are caused by improper packing Zenyth 200 User s Manual v 1 0 August 2010 6 blochrom e CAUTION Operating the Zenyth 200st without a ferrite bead on the printer cable when the printer is connected to the parallel printer port on the instrument may result in interference that affects the performance of other nearby devices e CAUTION It is recommended to use an IBM mouse model number 12J3618 with the Zenyth 200st If a different mouse is used the instrument may experience RFI or EFT Burst problems e CAUTION Operating the Zenyth 200st without a ferrite bead on the Y adapter when a keyboard and or mouse is connected may result in interference that affects the performance of other nearby devices e WARNING Turn off power to the instrument before replacing the air filter Removing the fan cover and air filter exposes the fan e CAUTION Depending upon the duration of the event transient power line interruptions may cause this equipment to lock up or reset with the possible loss of in process data If you suspect this has occurred and the equipment is not responding to commands manually cycle the power to the unit and repeat in process programs or runs e WARNING Turn off power and disconnect the instrument from the power source before performing maintenance on the Zenyth 200 Risk of electric shock when the
6. 062 Type 111 COST96 STANDARD COST384 Bode r Firmware Version Temperature V1 1 02 11 2005 Read Temperature 23 6 LR Set Temperature r Service Code Type Celsius z IE Zenyth 200 User s Manual v 1 0 August 2010 13 blochrom 3 To measure a plate Go to Reading gt Quick or the R button in the task bar To confirm that the instrument is connected with the computer select the Read Configuration button The serial number of the instrument should now appear in the Setup gt Instrument dialogue box along with compatible plate types Quick Read Load Plate Eject Plate Format Microplate In the Quick Read dialogue box Plate Type Confirm that the correct format and Measurement Position AI Iv Set Sample plate type are selected Measurement Mode Endpoint Photometric v vY Select All in Measurement Position to read the entire plate Vv Select Endpoint Photometric for basic readings using a Measurement Filter Reference Filter measurement and reference filter Cancel Select the measurement filter and the reference filter from the drop down menu v Note It is important to use a reference filter to account for optical inference from the plate Place plate with A1 in the upper left corner of the plate transport Select Start Absorbance measurements will appear in the open matrix in ADAP Data can be exported to data analysis software using the Copy icon Data wil
7. A 12 2 2 4 Loading a Microplate Into the Zenyth 200rt eke keke Eke ke kek kek K KAKA KAKE KAKE KA AA AA 13 2 2 5 Loading a Cuvette Into the Zenyth 200rt keke keke ke keke kake kK Ak Kr A KARA KA KAKE KA KA KA A KA 15 2 rrrrC er r r r e o a r om _er gt eJJv r r r r rXerebrrrlkbr rbWz 16 3 1 Performing Preventive MaintenanCce l l kek ekkk kk kk Eke kA KK AKA EKA KA NAKA HEKA HEKA HEKA HEKA HEKA WAK AWA KWA 16 3 2 Calibrating Lamp Brightness and the Plate and Optics TranSDOF S Eu EEE kk kk kek kk YA 16 34 Replacing the Fan BIlt yay1 oi y Ke n eh nlne adan suk Hee N H k ra nee de wC AYE KAR K Kwa N DAD N Hesek kane Kek n wa elek laie 17 3 5 Replacing the Deuterium Lamp i lule lcisekaeedikekakes kekake keka ee lkek k kaeak cae k n kulen e saa we W k kek irin 18 3 7 Tr ulbleshGOt g i i i d cnn d bi ybee dil leda nanase d d sihka a b e dada da ekin a ne y eE kd wekin kana a n n dya k 25 Appendix A SpeGIfFICat OD isxi xilalko s le kirye k lek Anka yanan ch R kewa ya s na k a d ra Hi an ka ava V sah ka san b Ad n n na 27 Zenyth 200 User s Manual v 1 0 August 2010 3 axana xarna La TEN N J zz Kr rd astata ni NaNe NANEN Eeee eaae Na anan 27 A 2sPhotometricSpecificationsmmnnsen mE ET 28 ACSICOMPULE SYSTEM REGUINEIMEMtS eccrarssrecteesteresesserce
8. CAL SAFETY Normal operation of the Zenyth 200 spectrophotometer may involve the use of materials that are toxic flammable or otherwise biologically harmful When using such materials observe the following precautions e Handle infectious samples according to good laboratory procedures and methods to prevent the spread of disease e Observe all cautionary information printed on the original solutions containers prior to their use e Dispose of all waste solutions according to your facility s waste disposal procedures e Operate the Zenyth 200 spectrophotometer in accordance with the instructions outlined in this manual and take all the necessary precautions when using pathological toxic or radioactive materials e Take appropriate safety precautions such as using safety glasses and wearing protective clothing when working with potentially hazardous liquids e Use an appropriately contained environment when using hazardous materials e Observe the appropriate cautionary procedures as defined by your safety officer when using flammable solvents in or near a powered up instrument e Observe the appropriate cautionary procedures as defined by your safety officer when using toxic pathological or radioactive materials e Note Observe all warnings and cautions listed for any external devices attached or used during operation of the Zenyth 200 spectrophotometer Refer to applicable external device user s manuals for operating proc
9. Figure 3 9 Fuse carrier location WARNING Turn off and unplug power to the instrument before changing fuses Failure to do so can cause electrical shock or equipment damage If the instrument will not power on or shuts down due to a power loss or if the fuses appear burned replace them Fuses are located in the fuse carrier on the back of the instrument next to the power switch and port Figure 3 9 To replace the fuses 1 2 Turn off the main power to the Zenyth 200 Unplug the power cord from the power port Using a small flathead screwdriver gently pry the fuse carrier loose and remove it from the instrument Gently pull old fuses from the carrier by hand Zenyth 200 User s Manual v 1 0 August 2010 23 olochrom 5 Gently place new fuses into carrier by hand Note Use 2 5A TH 250VAC slow blow type fuses for replacement 6 Replace the fuse carrier 7 Plug the power cable into the power port 8 Turn on power to the instrument Note If the instrument still does not power on after changing fuses contact an Anthos Service Engineer Zenyth 200 User s Manual v 1 0 August 2010 24 blochrom 3 7 Troubleshooting WARNING Only Anthos Service Engineers that are officially trained may perform service procedures on the instrument Problem Possible Solution If the indicator light is out Check Fuses check fuses If the Power is on but the Check fuses instrument does not operate The fuses appea
10. Refer to Section 3 5 Replacing the Deuterium Lamp for information about replacing the deuterium lamp To replace the halogen lamp WARNING Turn off power and disconnect the instrument from the power source before performing maintenance on the Zenyth 200 Risk of electric shock exists when the power is on WARNING Turn off the instrument and allow a minimum of 30 minutes for the lamp to cool before servicing During operation the halogen lamp reaches a temperature of approximately 300 C 572 F 1 Turn off power and disconnect the power cable from the instrument 2 Remove the two Allen screws on the back of the instrument that secure the lamp housing cover in place 3 Open the lamp housing cover by swinging it down from the back of the instrument The base of the halogen lamp halogen lamp socket orientation clip and lamp block assembly are clearly visible Figure 3 6 Figure 3 6 Halogen lamp and lamp block assembly 4 Unplug the halogen lamp socket from the base of the halogen lamp Figure 3 7 Zenyth 200 User s Manual v 1 0 August 2010 21 olochrom Figure 3 7 Halogen lamp socket disconnected from halogen lamp base CAUTION Do not loosen or attempt to remove the 4 screws on top of the lamp block assembly Doing so will result in a misalignment of the optical path 5 Loosen but do not remove the Allen screw that fastens the lamp orientation clip to the lamp block assembly Figure 3 8 The screw is loc
11. acing the halogen lamp To replace the deuterium lamp WARNING Turn off power and disconnect the instrument from the power source before performing maintenance on the Zenyth 200 Risk of electric shock exists when the power is on WARNING Never view the lamp directly without approved eyewear Protect skin from exposure to the light emitted by the lamp The deuterium lamp emits UV radiation WARNING Turn off the instrument and allow a minimum of 30 minutes for the lamp to cool before servicing During operation the deuterium lamp reaches a temperature of approximately 250 C 482 F 1 Turn off power and disconnect the power cable from the instrument Zenyth 200 User s Manual v 1 0 August 2010 18 biochrom Remove the two Allen screws on the back of the instrument that secure the lamp housing cover in place Figure 3 2 Figure 3 2 Removing the allen screws 3 Open the lamp housing cover by swinging it down from the back of the instrument The base of the deuterium lamp power cable and power cable connector are clearly visible Figure 3 3 Figure 3 3 Deuterium lamp and power cable 4 On the power cable connector press down on the top of the connector key to unlatch it then disconnect the lamp power cable from the power cable connector Figure 3 3 Zenyth 200 User s Manual v 1 0 August 2010 19 blochrom Figure 3 4 5 Remove the two flathead screws that secure the deuterium lamp Figure 3 4
12. aminated Zenyth 200 User s Manual v 1 0 August 2010 9 blochrom 2 1 INSTRUMENT OVERVIEW The Zenyth 200rt is a computer controlled spectrophotometer that performs photometric measurements on samples in 6 to 384 well microplates and cuvettes Figure 1 1 A high precision monochromator allows the Zenyth 200rt to perform endpoint kinetic multiwavelength and scan measurements at wavelengths ranging from 190 to 1000 nm The instrument also provides temperature control and shaking for microplate samples The Zenyth 200rt is operated remotely through an external computer using the supplied ADAP Software The ADAP Software can configure and perform photometric measurements using the Zenyth 200rt and save and evaluate measurement results The ADAP Software may be upgraded to the ADAP Prisma Software which provides additional qualitative and quantitative measurement and evaluation capabilities Figure 1 1 The Zenyth 200rt spectrophotometer 2 2 SETTING UP AND OPERATING THE ZENYTH 200RT 2 2 1 Unpacking and Setting Up the Zenyth 200rt Place the Zenyth 200rt on any dry flat work area that has sufficient space for the instrument external computer and required cables The Zenyth 200rt packaging is specifically designed to protect the instrument during transport Keep the original carton foam inserts and accessories box in case the instrument needs to
13. ated between the orientation clip tab and the base of the halogen lamp Figure 3 8 Halogen lamp orientation clip and lamp block assembly 6 Grasp the tab of the lamp orientation clip and remove the clip by sliding it straight up from the lamp block assembly WARNING Handle the old halogen lamp with caution The lamp may be hot 7 Remove the halogen lamp and discard CAUTION Do not touch the glass of the new halogen lamp Finger prints on the glass may shorten the life of the lamp 8 Taking care not to touch the glass bulb with bare hands install the new halogen lamp with the prongs aligned horizontally Figure 3 8 9 Install the lamp orientation clip so the groove slides down over the fastening screw and the tab protrudes towards the rear of the instrument Zenyth 200 User s Manual v 1 0 August 2010 22 10 11 12 13 14 15 olochrom While pressing down lightly on the lamp orientation clip to maintain proper alignment tighten the Allen screw that fastens the orientation clip to the lamp block assembly Plug the lamp socket into the base of the halogen lamp Close the lamp housing cover Tighten the two allen screws to secure the lamp housing cover in place Reconnect the power cable and turn on power to the instrument Calibrate the brightness of the new lamp refer to Section 4 2 5 1 Calibrating Lamp Brightness 3 6 Replacing the Fuses
14. bols 1 1 WARNING AND CAUTION DEFINITIONS The exclamation point symbol is an international symbol which serves as a reminder that all safety instructions should be read and understood before installation use maintenance and servicing is attempted When this symbol is displayed in this manual pay special attention to the specific safety information associated with the symbol WARNING A WARNING calls attention to a condition or possible situation that could cause injury to the operator A CAUTION calls attention to a condition or possible situation that could damage or destroy the product or the operator s work To prevent electrically related injuries and property damage properly inspect all electrical equipment prior to use and immediately report any electrical deficiencies Contact an Anthos service representative for any servicing of equipment requiring the removal of covers or panels High Voltage This symbol indicates the potential of an electrical shock hazard existing from a high voltage source and that all safety instructions should be read and understood before proceeding with the installation maintenance and servicing of all modules Do not remove system covers To avoid electrical shock use supplied power cords only and connect to properly grounded three holed wall outlets Do not use power strips The WARNINGs and CAUTIONS found within this document are listed below e CAUTION Turn off main power to
15. ct tort including negligence breach of warranty strict liability or otherwise and including but not limited to damages resulting from loss of data loss of anticipated profits or any special indirect incidental or consequential damages In no event shall Biochrom Ltd s liability to the user exceed the amount paid by the user to Biochrom Ltd The user assumes full responsibility for the results obtained from the use of this system and related documentation and for application of such results Microsoft Windows and Windows NT are either registered trademarks or trademarks of Microsoft Corporation in the United States and or other countries IBM is a trademark of International Business Machines Corporation in the United States other countries or both Pentium is a registered trademark of Intel Corporation All other trademarks appearing in this manual are owned by their respective companies Copyright 2003 by Biochrom Ltd All rights reserved No part of this publication may be reproduced distributed or transmitted in any form or by any means electronic mechanical photocopying recording or otherwise or stored in a database or retrieval system without the prior written permission of Biochrom Ltd Note The contents of this manual are subject to change without notice Zenyth 200 User s Manual v 1 0 August 2010 2 TABLE OF CONTENTS TABIATO NGSTIETE N Z TE TT NNE NE E EE DD EE E EEE NNN 3 Warranty and Returned Good
16. edures of that device Zenyth 200 User s Manual v 1 0 August 2010 8 blochrom 1 3 MOVING PARTS To avoid injury due to moving parts observe the following e Never attempt to exchange lab ware reagents or tools while the instrument is operating e Never attempt to physically restrict any of the moving components of the Zenyth 200 spectrophotometer e Keep the Zenyth 200 spectrophotometer work area clear to prevent obstruction of the movement 1 4 CLEANING Observe the cleaning procedures outlined in this user s manual for the Zenyth 200 spectrophotometer Prior to cleaning equipment that has been exposed to hazardous material e Appropriate Chemical and Biological Safety personnel should be contacted e The Chemical and Biological Safety information contained in this user s manual should be reviewed 1 5 MAINTENANCE Perform only the maintenance described in this manual Maintenance other than that specified in this manual should be performed only by Anthos service representatives It is your responsibility to decontaminate components of the Zenyth 200 microplate reader spectrophotometer before requesting service by a Anthos service representative or returning parts to Anthos for repair Anthos will NOT accept any items which have not been decontaminated where it is appropriate to do so If any parts are returned they must be enclosed in a sealed plastic bag stating that the contents are safe to handle and are not cont
17. ibrated every 1 000 measurements 3 3 Using the ADAP Software to Calibrate the Instrument Administrators Level 2 and system administrators Level 3 may use the ADAP Software to perform lamp output and transport calibrations e Adjust Lamp checks the light output of the lamps and adjusts lamp output and gain as needed for all wavelengths Zenyth 200 User s Manual v 1 0 August 2010 16 blochrom e Auto Calibration calculates the home positions for the plate and optics transports To calibrate the instrument 1 In the ADAP Software open Functions 2 and choose Adjust Lamp to check and adjust the output of the lamps and gain levels 2 Choose Auto Calibration to calculate the zero positions for the plate and fork transports Note Refer to Chapter 4 of the ADAP Software Operating Manual for more information about the calibration functions 3 To calibrate the plate and optics transports in System Setup choose Auto Calibration refer to Section 4 2 1 Performing Instrument and Microplate Operations 3 4 Replacing the Fan Filter The Zenyth 200 has a cooling fan with a filter The filter should be changed after every 500 hours of use Note ADAP Software keep track of the time elapsed between filter changes and display a visual prompt when the filter is due to be replaced WARNING Turn off power to the instrument before replacing the fan filter Removing the fan cover and filter exposes the fan To replace the fan f
18. ilter 1 Turn off power and disconnect the power cable from the instrument 2 Using a small flathead screwdriver pry the fan cover off the back of the instrument Figure 3 1 The fan cover protective screen and fan filter come off the instrument together Figure 3 1 Changing the fan filter Zenyth 200 User s Manual v 1 0 August 2010 17 blochrom 3 Install the new filter 4 Press the fan cover screen and filter back into position on the instrument 5 Reconnect the power source and turn on power to the instrument 6 If the ADAP Software controls the instrument open Functions 1 and choose Reset Air Filter to reset the counter that tracks the time elapsed between air filter changes refer to Chapter 4 of the ADAP Software Operating Manual Note Reset Air Filter may only be performed by users with administrator Level 2 or system administrator Level 3 privileges 3 5 Replacing the Deuterium Lamp The Zenyth 200 is equipped with a deuterium lamp which is used for generating light in the UV range from 190 to 325 nm Note The ADAP Software and Zenyth 200st standalone software report a Deuterium lamp defect when the lamp needs to be changed Replacing the deuterium lamp requires a 2 5 mm Allen wrench or hex screwdriver ballpoint type recommended and a 2 5 x 0 6mm flathead screwdriver Both tools are provided with the instrument Note Refer to Section 3 6 Replacing the Halogen Lamp for information about repl
19. l paste as a matrix with filter wavelength time and date Zenyth 200 User s Manual v 1 0 August 2010 14 olochrom 1 Inthe ADAP Software from the toolbar choose Eject Plate to move the plate carrier outside the instrument 2 Place the microplate on the plate carrier with well A1 oriented so that it is in the back left corner of the plate carrier Figure 1 4 i Figure 1 4 Zenyth 200rt with plate carrier open 3 In the ADAP Software choose Load Plate to move the plate carrier inside the instrument 2 2 5 Loading a Cuvette Into the Zenyth 200rt Cuvettes to be read are loaded manually into the cuvette holder near the back left corner of the instrument Figure 1 4 When performing measurements cuvettes are not loaded into the instrument until the lamp output has stabilized During measurements the ADAP Software displays a visual prompt onscreen when the cuvette should be inserted in the holder Note Refer to Chapter 6 in the ADAP Software Operating Manual for more information about performing measurements on cuvette samples To load a cuvette into the Zenyth 200rt 1 Open the door to the cuvette holder by hand Figure 1 4 Zenyth 200 User s Manual v 1 0 August 2010 15 olochrom 2 When prompted by the ADAP Software load the cuvette into the cuvette holder with the clear sides facing to the left and right side
20. olochrom oe Zenyth 200 Microplate Reader and Spectrophotometer User s Manual Biochrom Ltd 22 Cambridge Science Park Cambridge UK CB4 OFJ Tel 44 1223 423723 E Mail enquiries biochrom co uk blochrom Except as provided in writing signed by an officer to Biochrom Ltd this system and any related documentation are provided as is without warranty of any kind expressed or implied including that the system is error free This information is presented in good faith but Biochrom Ltd not warrant guarantee or make any representations regarding the use or the results of the use of this system and related documentation in terms of correctness accuracy reliability omissions or otherwise The entire risk as to the use results and performance of this system and related documentation is assumed by the user Except as expressly provided herein Biochrom Ltd makes no other warranty whether oral or written expressed or implied as to any matter whatsoever including but not limited to those concerning merchantability and fitness for a particular purpose nor is freedom from any patent owned by Biochrom Ltd or by others to be inferred LIMITATIONS OF LIABILITY Biochrom Ltd shall not be liable to any extent whatsoever for any damages resulting from or arising out of the use or performance of this system and related documentation or the procedures specified in this manual the form of action whether in contra
21. onment Power Requirements Dimensions W x H x L Weight Ambient Operating Temperature Ambient Storage Temperature Humidity Restrictions Altitude Restrictions up to 2000 m 6 562 ft Fuses Data Connections Table A 1 Instrument Specifications Zenyth 200 User s Manual v 1 0 b Description Indoor use only 100 240VAC 50 60Hz 47 5 cm x 26 4 cm x 44 cm 18 7 in x 10 4 in x17 3 in 16 5 kg 36 4 Ibs 15 aloe 5S E 25 50 C 13 122 F 15 75 non condensing 30 C 86 F ochrom 2 Air Pressure Restrictions 54 106 kPa 7 8 15 4 PSI 2 5A TH250VAC 5x20 mm slow blow UL recognized CSA VDE RS 232 9 pin serial August 2010 27 blochrom A 2 Photometric Specifications Table A 2 Photometric Specifications Item Description _ Wavelength range 190 1000 nm Bandwidth 5 nm Resolution 0 1 mOD Indication range 0 000 4 000 OD endpoint measurement Reproducibility lt 0 5 at 1 OD and 2 OD 400 750 nm lt 0 7 at 1 OD 190 399 nm and 751 1000 nm Linearity lt 0 7 and 0 005 OD from 0 1 3 0 OD 400 750 nm lt 0 7 and 0 005 OD from 0 1 2 0 OD 190 399 nm and 751 1000 nm Accuracy lt 1 5 at 1 OD Wavelength reproducibility 0 5 nm Wavelength accuracy 2 nm Warm up time 30 minutes to reach specifications Zenyth 200 User s Manual v 1 0 August 2010 28 b A 3 Computer Sy
22. port on the back of the instrument Figure 1 2 Zenyth 200 User s Manual v 1 0 August 2010 11 Figure 1 2 Power switch and connection ports 5 Connect the power cable to the power port on the back of the instrument Figure 1 2 6 Turn on the power switch on the back of the instrument Figure 1 2 Note For optimal performance allow the Zenyth 200rt to warm up for 30 minutes before performing measurements 2 2 3 Setting Up and Operating the Zenyth 200rt 1 On the external computer start the ADAP Software 2 Log into the ADAP Software as an administrator Level 2 or system administrator Level 3 user name admin sadmin password admin sadmin 3 Inthe ADAP Software from the Setup menu choose Instrument Instrument appears Figure Instrument Seles 1 3 File Instrument Functions 1 Functions 2 Down Upload Fite tae In the ADAP Software configure the instrument settings kurm KEKE SE by following the steps in Chapter 3 of the ADAP Instrument Type T Zenn d p Installed Plates Zereth200 Software Operating Manual r Head Configuration 12 COST12 heed a a ig costae Serial Number 96 BC_96_F a 96 COST96 be Figure 1 3 ADAP Software 36 STANDARD 384 COST384 Barcode r l Temperature _ Firmware Version Read Temperature 29 2 L Set Temperature Service Code Type Celsius v OK V1 1 11 02 2005 Zenyth 200 User s Manual v 1 0 Augu
23. r burned or Replace fuses refer to Section 3 7 Replacing the Fuses filaments are broken The indicator light is out the Contact Biochrom service engineer power on the fuses are intact and the instrument does not operate The instrument does not Depending upon the duration of the event transient power respond to commands interruptions may cause this equipment to lock up or reset with the possible loss of in process data Manually cycle the power to the instrument and repeat in process programs or runs Table 3 1 Troubleshooting the Zenyth 200 3 8 Storing the Zenyth 200 To store the Zenyth 200 return it to the original packaging and store in a dry dust free environmentally controlled area 3 9 1 Repacking the Zenyth 200 for Transport The Zenyth 200 should always be placed in the original packaging for transportation by ground or aerial shipment CAUTION Warranty claims are void if transport damages are caused by improper packing To repack the Zenyth 200 for shipping Zenyth 200 User s Manual v 1 0 August 2010 25 blochrom 1 Turn off power to the instrument 2 Disconnect the power cable from the wall socket and power port on the back of the instrument 3 If repacking a Zenyth 200rt disconnect the serial cable from the serial ports on the external computer and instrument Zenyth 200 User s Manual v 1 0 August 2010 26 APPENDIX A SPECIFICATIONS A 1 Instrument Specifications Item Envir
24. rceccceresicsesuusccverevceceusevacter Nere nere eee bereke reee rere er eee 29 WARRANTY AND RETURNED GOODS REQUIREMENTS All standard Biochrom Ltd policies governing returned goods apply to this product Subject to the exceptions and upon the conditions stated below the Company warrants that the products sold under this sales agreement shall be free from defects in workmanship and materials for one year after delivery of the products to the original Purchaser by the Company and if any such product should prove to be defective within such two years period the Company agrees at its option either 1 to correct by repair or at the Company s election by replacement any such defective product provided that investigation and factory inspection discloses that such defect developed under normal and proper use or 2 to refund the purchase price The exceptions and conditions mentioned above are as follows a Components or accessories manufactured by the Company which by their nature are not intended to and will not function for one year are warranted only to reasonable service for a reasonable time What constitutes a reasonable time and a reasonable service shall be determined solely by the Company A complete list of such components and accessories is maintained at the factory b The Company makes no warranty with respect to components or accessories not manufactured by it In the event of defect in any such component or accessory the Company
25. s Requirements seseerseeser rese vone cere eme vene cene yene we Hew Wene eo we ene 4 The SETI YOO CUETO ae E 6 1 1 Warning and Caution Definitions cccccccssccsssscsessecssscecsecessaeesessecseeeesaeeesaaeeseseesseeeessaeesssaeesenees 6 1 2 Chemical and Biological Safety cccsccssssccssssesssecssssecseeeecsesecsueesesseceeeessaeesssaeessseessesessaeessaeesenees 8 1 3 MOVING Patt opisao isian A GhaweesswascusGetcaececsacedeloacnavevebussgedveacaveeeveaeaelunceeviees vosueiGensauvessecsaeloeces 9 e E A E PE E AAA E E E E EN A E N E E E T 9 tr aa t DD airs 9 231 aba d Dal Zer r r gt r a a m 10 2 2 Setting Up and Operating the Zenyth 200rt EE kk xek k ke kak ke k kr ka k KA Ka KA KK KA 10 2 2 1 Unpacking and Setting Up the Zenyth 200rt keke keke ke kake E kek KK KAKA KA KAKE KAKA KK AA KA 10 To unpack ithe Zenyth 2005 4 xi ji x k y i selek da rk ak cle le lekana kaba Mal aie cia e n d ka peyak A a k k b d exa n d n eka d san k ki 11 2 2 2 Setting Up and Operating the Zenyth 200rt kek eke keke kake A kek KK A KK AKA KAKA KAKA KK AA AA 11 To set Up the Zenyth 200M i i 4i lt 1y 15 4salak kelk n ken de reaksie k d b h k na e d k a aye na ca he aiaia k p aaaea KER N 11 2 2 3 Setting Up and Operating the Zenyth 200rt keke keke keke keke E kek K k AK KAKA KAKA KAKE KA KAK
26. s of the instrument Note The cuvette must be oriented with the clear sides facing to the left and right sides of the instrument Note The cuvette holder door does not need to be closed when cuvettes are being measured 3 When the ADAP Software displays a visual prompt that the measurement is complete remove the cuvette from the cuvette holder 3 MAINTENANCE Several maintenance procedures may be performed by users to ensure the optimum operation of the Zenyth 200 These procedures include 3 1 Performing Preventive Maintenance To ensure optimum operation of the Zenyth 200 perform the following preventive maintenance procedures as necessary e Wipe up any spills immediately e Follow appropriate decontamination procedures as instructed by the laboratory safety officer e Contact an Anthos Service Engineer to inspect the instrument every two years Use special test plates to regularly verify the performance of the instrument Contact an Anthos Service Engineer for more details 3 2 Calibrating Lamp Brightness and the Plate and Optics Transports Users may calibrate the brightness of the lamps and the home positions of the plate and optics transports Calibration must be performed when a lamp is replaced an internal mechanical optical or electronic component is repaired or a crash occurs during plate carrier movement Calibration may be performed using the ADAP Software Note The brightness of the lamps is automatically cal
27. st 2010 12 blochrom 4 After turning on the power switch at the rear of the instrument a power indicator on the front panel indicates the current status of the instrument Figure 1 4 e Continuous green the instrument is ready for operation e Flashing green a problem occurred during initialization the instrument is not ready for operation e Not illuminated the instrument is not ready for operation Note For optimal performance allow the Zenyth 200rt to warm up for 30 minutes before performing measurements 2 2 4 Loading a Microplate Into the Zenyth 200rt Microplates to be read are placed on the plate carrier in the front of the instrument To load a microplate into the instrument 1 To connect instrument to ADAP software Insert CD supplied with the instrument into PC install ADAP Open ADAP ADAP will prompt for a user ID and password Use the pre set ID and passwords are sadmin sadmin Once logged as sadmin set specific user IDs passwords and administrative rights Select Setup gt Instrument in the menu bar A dialogue box will open Instrument File Instrument Functions 1 Functions 2 Down Upload Under the Instrument tab la fas v In Baudrate select Auto Sense Baudrate Auto Sense v J Warseno 000m owra o F In COM Port select port or Auto Sense v In Instrument Type select Zenyth200 in the drop Instrument Type a a down menus COST6 Read Configuration Serial Number 1
28. stem Requirements Component CPU Pentium Pentium II RAM Hard Drive CD ROM Drive Monitor Keyboard Mouse Serial Port Operating Systems Web Browser Minimum Requirements 133 Mhz minimum 500 Mhz recommended 16 MB minimum 64 MB recommended 50 MB free space 4X 640x480 resolution 101 key IBM compatible 1 free serial port per instrument connected Windows 95 Y2K update required Windows 98 Y2K update 2 required Windows 98 Second Edition Windows Millennium Edition Windows NT 4 Service Pack 5 or higher Windows 2000 Windows 7 Windows XP Internet Explorer 4 01 Service Pack 2 or later Table A 3 Computer System Requirements Zenyth 200 User s Manual v 1 0 August 2010 2 ochrom 29
29. will give reasonable assistance to Purchaser in obtaining from the manufacturer s own warranty c Any product claimed to be defective must if required by the Company be returned to the factory transportation charges prepaid and will be returned to Purchaser with transportation charges collect unless the product is found to be defective in which case the product must be properly decontaminated of any chemical biological or radioactive hazardous material d The Company shall be released from all obligations under all warranties either expressed or implied if any product covered hereby is repaired or modified by persons other than its own authorized service personnel unless such repair by others is made with the written consent of the Company Zenyth 200 User s Manual v 1 0 August 2010 4 e olochrom e If the product is a reagent or the like it is warranted only to conform to the quantity and content and for the period but not in excess of one year stated on the label at the time of delivery It is expressly agreed that the above warranty shall be in lieu of all warranties of fitness and of the warranty of merchantability and that the company shall have no liability for special or consequential damages of any kind or from any cause whatsoever arising out of the manufacture use sale handling repair maintenance or replacement of any of the products sold under the sales agreement f Representatives and warranties made
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