Hardware User`s Manual Automated black & white test box
Contents
1. Once the cable is connected if the system detects that a load cell is damaged it will warn the user by flashing the red led that belongs to this load cell see Figure 6 Automated black amp white test box 16 Panlab Malis iy 10 RECOMMENDATIONS 1 It will be periodically necessary to clean the experimentation cage 2 Floors can be removed for cleaning Prior to removing them turn off the control unit and gently lift them up to free the 4 legs form the load cell holes in the platform 3 Once you return floors to the cage check that they are correctly placed the four legs of the floor must be in the load cell supports to correctly transmit weight to the load cell 4 System auto balance must be done without animals on the floor the system detects floor weight during auto balance and balances it to detect animal weight 5 Although this is not strictly necessary it is better to wait 10 minutes after having turned on the equipment before placing animals on the floor the system will reach a permanent thermal state in this period 10 1 WALLS CLEANING To clean the walls you can use a lightly wet cloth and then dry them with a dry cloth If they re too dirty you can wet the cloth with a soapy solution to clean them then remove foam with a wet cloth and finally dry them with a dry cloth 10 2 FLOORS CLEANING To clean the floors you can use a lightly wet cloth and then dry them with a dry cloth If they re too dir2. Automated black amp white test box Panlab 4 MAINTENANCE WARNING Failure to follow the instructions in this section may cause equipment fault e PRESS KEYS SOFTLY Lightly pressing the keys is sufficient to activate them e Equipments do not require being disinfected but cleaned for removing urine faeces and odour To do so we recommend using a wet cloth or paper with soap which has no strong odour NEVER USE ABRASIVE PRODUCTS OR DISSOLVENTS e NEVER pour water or liquids on the equipment e Once you have finished using the equipment turn it off with the main switch Clean and check the equipment so that it is in optimal condition for its next use e The user is only authorised to replace fuses with the specified type when necessary OPENING FLANGE SWITCH FUSE HOLDER Figure 1 Power inlet main switch and fuse holder FUSE REPLACEMENT In case of an over voltage or other incident in the AC net making it impossible to turn on the equipment check fuses according to the following procedure 1 Remove power cord from the power inlet Automated black amp white test box 2 Harvard CN Apparatus 2 Open fuse holder by pulling the flange with a regular screwdriver Figure 2 Open fuse holder door 3 Extract fuse holder using the screwdriver Figure 3 Extract fuse holder 4 Replace fuses if necessary Insert fuses in the fuse holder in the correct position CORR
3. e The REMOTE port of every unit is always connected to the MAIN port of the next control unit e The REMOTE port of the last control unit is left free Automated black amp white test box 15 Panlab pb 9 WORKING WITH THE EQUIPMENT 9 1 CONDUCTING AN EXPERIMENT The following procedure must be followed to work with the equipment 1 Connect all the cables see chapter 8 in this manual Connect the control unit to the AC network and turn it on Wait a few seconds until the READY led stays continuously on It will flash during auto calibration If you are working with a rat s cage you must select Rat on the rear panel selector If you are working with a mice cage you must select Mouse on the same selector Place the experimental subject in the cage Follow the PPCWin program indications for experimenting with the animal Remove the experimental subject from the cage To work with the next animal return to point number 5 and repeat procedure Once the experiment has finished turn offthe control unit and clean the cage 9 2 AUTOMATIC ERROR WARNING The system checks if the cable between the rear panel connector labelled Box and the cage is connected If the system detects this error it will warn you by flashing the 2 red position leds on the front panel see Figure 6 The system will be waiting for the problem to be solved connect the cable After the problem is solved the user must turn on the system again
4. Directive CEM 2012 19 EU La Directiva de Residuos de Aparatos El ctricos y Electr nicos WEEE The Waste Electrical and Electronic Equipment Directive WEEE Les d chets d quipements lectriques et lectroniques WEEE 2011 65 EU Restricci n de ciertas Sustancias Peligrosas en aparatos el ctricos y electr nicos ROHS Restriction of the use of certain Hazardous Substances in electrical and electronic equipment ROHS Restriction de l utilisation de certaines substances dangereuses dans les quipements lectriques et lectroniques ROHS 2006 42 EC Directiva mecanica Machinery directive Directive m canique Para su evaluacion se han aplicado las normas armonizadas siguientes For its evaluation the following harmonized standards were applied Pour son valuation nous avons applique les normes harmonis es suivantes Seguridad Safety S curit EN61010 1 2011 EMC EN61326 1 2012 Class A Safety of machinery EN ISO 12100 2010 This equipment complies with the limits for class A equipment in accordance with CISPR 11 definition and is classed as a Class A digital device pursuant to CFR Title 47 part 15 of the FCC Rules and is intended to be used in an industrial environment En consecuencia este producto puede incorporar el marcado CE Consequently this product can incorporate the CE marking En consequence ce produit peut incorporer le marquage CE En representacion del fabricante Manufacturer s rep
5. recogida municipales o devolverlos al lugar donde los adquiri Los detalles quedaran definidos por la ley de cada pa s El s mbolo en el producto en las instrucciones de uso o en el embalaje hace referencia a ello Gracias al reciclaje a la reutilizaci n de materiales a otras formas de reciclaje de usados usted contribuir de forma importante a la protecci n de nuestro medio ambiente I lt Remarques concernant la protection de l environnement Conform ment la directive europ enne 2002 96 CE et afin d atteindre un certain nombre d objectifs en mati re de protection de l environnement les r gles suivantes doivent tre appliqu es Elles concernent les d chets d quipement lectriques et lectroniques Le pictogramme picto pr sent sur le produit son manuel d utilisation ou son emballage indique que le produit est soumis cette r glementation Le consommateur doit retourner le produit usager aux points de collecte pr vus cet effet peut aussi le remettre un revendeur En permettant enfin le recyclage des produits le consommateur contribuera la protection de notre environnement C est un acte cologique D Hinweis zum Umweltschutz Ab dem Zeitpunkt der Umsetzung der europ ischen Richtlinie 2002 96 EU in nationales Recht gilt folgendes Elektrische und elektronische Ger te d rfen nicht mit dem Hausm ll entsorgt werden Der Verbraucher ist gesetzlich verpflichtet elektrische und elek
6. Transducer Exc 10V Transducer Exc Gnd Automated black amp white test box 20 Panlab H Harvard Apparatus DIMENSIONS CONTROL UNIT Width x Height x Depth 285mm x 70MM x 250mm Weight 1 84 kg DIMENSIONS TEST BOX Model Animal Compartments _ Lowe Opening Black White 20 X 31 24 2 i Dimensions expressed in centimetres Automated black amp white test box 21 Panlab pb DECLARACI N DE CONFORMIDAD DECLARATION OF CONFORMITY DECLARATION DE CONFORMIT Nombre del fabricante Panlab s l u Manufacturer s name www panlab com Nom du fabricant info panlab com Direccion del fabricante Energia 112 Manufacturer s address 08940 Cornell de Llobregat Adresse du fabricant Barcelona SPAIN Declara bajo su responsabilidad que el producto BLACK amp WHITE TEST BOX Declares under his responsibility that the product D clare sous sa responsabilit que le produit Marca Brand Marque PANLAB Modelo Model Modele LE 816 LE 818 Cumple los requisitos esenciales establecidos por la Uni n Europea en las directivas siguientes Fulfils the essential requirements established by The European Union in the following directives Remplit les exigences essentielles tablies pour l Union Europ enne selon les directives suivantes 2006 95 EC Directiva de baja tensi n Low Voltage Basse tensi n 2004 108 EC Directiva EMC EMC Directive
7. A 2 GOOD LABORATORY PRACTICE Check all units periodically and after periods of storage to ensure they are still fit for purpose Investigate all failures which may indicate a need for service or repair Good laboratory practice recommends that the unit be periodically serviced to ensure the unit is suitable for purpose You must follow preventive maintenance instructions In case equipment has to be serviced you can arrange this through your distributor Prior to Inspection Servicing Repair or Return of Laboratory Equipment the unit must be cleaned and decontaminated Decontamination prior to equipment disposal In use this product may have been in contact with bio hazardous materials and might therefore carry infectious material Before disposal the unit and accessories should all be thoroughly decontaminated according to your local environmental safety laws Automated black amp white test box Panlab 3 UNPACKING AND EQUIPMENT INSTALATION WARNING Failure to follow the instructions in this section may cause equipment faults or injury to the user A No special equipment is required for lifting but you should consult your local regulations for safe handling and lifting of the equipment B Inspect the instrument for any signs of damage caused during transit If any damage is discovered do not use the instrument and report the problem to your supplier C Ensure all transport locks are removed before use The orig
8. ECT INCORRECT Figure 4 Fuses position 5 Insert again fuse holder both possible positions are correct because power supply is universal 6 Ifthe fuses blow again unplug the equipment and contact technical service N For electrical safety never open the equipment The power supply WARNING has dangerous voltages Automated black amp white test box Panlab 5 TABLE OF CONTENTS 10 10 1 10 2 11 12 13 SYMBOLS TABLE GOOD LABORATORY PRACTICE UNPACKING AND EQUIPMENT INSTALATION MAINTENANCE TABLE OF CONTENTS INTRODUCTION EQUIPMENT DESCRIPTION CONTROL UNIT FRONT PANEL CONTROL UNIT REAR PANEL EXPERIMENTATION CAGE EQUIPMENT CONNECTION CONTROLLING 1 CAGE CONTROLLING SEVERAL CAGES WORKING WITH THE EQUIPMENT CONDUCTING AN EXPERIMENT AUTOMATIC ERROR WARNING RECOMMENDATIONS WALLS CLEANING FLOORS CLEANING TROUBLESHOOTING PREVENTIVE MAINTENANCE TECHNICAL SPECIFICATIONS Automated black amp white test box Harvard Apparatus 10 10 11 12 13 13 14 16 16 16 17 17 17 18 19 20 Panlab H 6 INTRODUCTION Numerous behavioural paradigms using several conflict procedures social interaction or exploration of novel environments have been proposed as animal anxiety models Costall et al have described Pharmacol Biochem Behav 32 3 777 785 1989 a new model based on the aversive properties of the open field in which anxiolytic drug in
9. Hardware User s Manual Automated black amp white test box References L E816 76 0008 LE818 76 0010 Version V23 10 2014 Panlab s l u International Calls 34 934 750 697 Pan H Harvar d C Energ a 112 Domestic Call 934 190 709 pparatus 08940 Cornella de Ll Barcelona Fax 34 934 750 699 Spain Info panlab com www panlab com Limitation of Liability PANLAB does not accept responsibility under any circumstances for any harm or damage caused directly or indirectly by the incorrect interpretation of what is expressed in the pages of this manual Some symbols may have more than one interpretation by professionals unaccustomed to their usage PANLAB reserves the right to modify in part or in total the contents of this document without notice Panlab Malis iy 1 SYMBOLSTABLE Recognising the symbols used in the manual will help to understand their meaning DESCRIPTION SYMBOL Warning about operations that must not be done because they can damage the equipment Warning about operations that must be done otherwise the user can be N exposed to a hazard Protection terminal ground connection D Warning about a hot surface which temperature may exceed 65 C AN Warning about a metal surface that can supply electrical shock when it s A touched Decontamination of equipments prior to disposal at the end of their operative life Waste Electrical and Electronic Equipment Directive WEEE
10. NTROLLING 1 CAGE Figure 9 Equipment Connections Figure 9 shows the connections used when working with one cage The necessary cables are outlined in the next table FROM CABLE Control unit MAIN Computer Serial Port RS 232 cable Control Unit BULBS Cage Bulbs 4 pins cable Control Unit BOX Cage platform DB25 to DIN 7 cable Automated black amp white test box 13 Panlab 8 2 CONTROLLING SEVERAL CAGES 2 cml w lo I Figure 10 Connections controlling several cages Automated black amp white test box 14 Panlab H computer can control up to 8 cages The connection between control units and the computer is as follows when working with several cages Figure 10 shows an example of a 3 cage connection Some important guidelines must be followed when working with more than one cage e Allthe units must have different IDs for the computer to identify them e 15 necessary for all ID numbers to be correlative For example it will be as correct to work with cages 1 2 and 3 as it will be to work with cages 1 5 and 7 e It is not necessary for cages to be physically located in the order of their ID number For example it will be equally correct to work with cages 1 2 3 1 3 2 2 1 3 2 3 1 3 1 2 Or 3 2 1 e The computer serial port is always connected to the MAIN port of the first control unit
11. duced ease of exploratory activity is compared between light and dark coloured compartments This model permits simple and quick evaluation of anxious behaviour and its modification by pharmacological agents Panlab has developed a complete system to carry out this experiment including both cages for the experimental subjects and the appropriate control instrumentation Figure 5 Black and White Test Box The cages are composed of 2 compartments with walls 24 cm high One of the compartments is smaller and coloured black and the other is larger and coloured white Both compartments are separated by a higher wall to act as a separator with an opening to allow the animal to pass from one side to the other This wall also supports the lighting system a 40W white bulb on the white side and a 25W red bulb on the black side Automated black amp white test box Panlab The two floors are easily removable They are made of Perspex in the same colour as their respective compartments and are marked in 9 sectors by lines The cages do not have a top lid Automated black amp white test box Panlab 7 EQUIPMENT DESCRIPTION 7 1 CONTROL UNIT FRONT PANEL READY WHITE BULB ANIMAL POSITION BULB BLACK amp WHITE LE adeo APPARATUS Figure 6 Control Unit Front Panel e READY When you turn on the control unit the ready led will flash a few seconds while the system is auto balancing When the sys
12. e serial port is correct Restart the equipment and the PC to do a RESET in communications Check in the rear panel of control unit that the ID number is the same that the one set in PPCWin 18 Panlab 12 PREVENTIVE MAINTENANCE WHEN NECESSARY WALLS CLEANING CHECK FLOORS PLACING AR M Bulbs must be replaced by ones with the same specs see especifications Automated black amp white test box 19 Panlab pb 13 TECHNICAL SPECIFICATIONS POWER SUPPLY Input voltage Frequency Fuse Maximum power Conducted noise Universal 100 240 VAC 50 60Hz 2 fuses 5mm 20mm 2 250V Fast 18W EN55022 CISPR22 CISPR16 class B ENVIRONMENTAL CONDITIONS Operating temperature Operating relative humidity Storage temperature 10 C to 40 C 0 to 85 RH non condensing o C to 50 C non condensing LIGHT White Bulb 40W 230V screw E27 Red Bulb 25W 230V screw E27 POSITION DETECTION Rat 30 gr Mouse 7 gr CONNECTOR BULB Pin Function A AC voltage B Right switch Not connected D Left switch CONNECTOR MAIN REMOTE Pin Function 2 Rxd 3 Txd 5 Gnd 7 Rts 9 Cts COMUNICATIONS OUTPUT Standard Interface RS232C Connector Transmission speed Delta 9 contacts connector 19200 bauds 8 bits no parity CONNECTOR BOX Pin 10 11 12 13 23 24 25 Function Transducer left S Transducer left 5 Transducer Right 5 Transducer left S
13. free e BULBS 4 pin connector used to power the white and red bulbs e BOX DB25 female connector used to connect the control unit to the experimentation cage It transmits load cell signals e RAT MOUSE Selector used to set the model of cage being used rat or mouse e POWER Power inlet main switch and fuse holder Automated black amp white test box 11 Panlab H 7 3 EXPERIMENTATION CAGE WHITE COMPARTMENT BULBS BLACK COMPARTMENT ee PLATFORM FLOOR Legs Figure 8 Experimentation Cage The experimentation cage has two compartments The left compartment is larger and coloured white The right compartment is smaller and coloured black The floors have the same colour as the walls of their respective compartments and are divided into 9 sectors with lines The experimentation cage is mounted on a platform Inside the platform there are 2 load cells that detect animal position The floor transmits weight to the load cell with 4 legs The floor is easily removable for cage cleaning Two bulbs are installed at the upper part of the cage a 40W white bulb for the white compartment and a 25W red bulb for the black compartment Shared Wall Opening Dimensions in cm The dimensions of the Rat and Mouse Experimentation Cages are listed in the foregoing table Automated black amp white test box 12 Harvard Apparatus Panlab H Harvard 8 EQUIPMENT CONNECTION 8 1 CO
14. inal packing has been especially designed to protect the instrument during transportation It is therefore recommended to keep the original carton with its foam parts and accessories box for re use in case of future shipments Warranty claims are void if improper packing results in damage during transport D Place the equipment on a flat surface and leave at least 10 cm of free space between the rear panel of the device and the wall Never place the equipment in zones with vibration or direct sunlight E Once the equipment is installed in the final place the main power switch must be easily accessible F Only use power cords that have been supplied with the equipment In case that you have to replace them the spare ones must have the same specs that the original ones G A Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits A For electrical safety reasons you only can connect equipment to power outlets provided with earth connections This equipment can be used in installations with category ll over voltage according to the General Safety Rules WARNING The manufacturer accepts no responsibility for improper use of the equipment or the consequences of use other than that for which it has been designed Automated black amp white test box Panlab Control Some of these instrume
15. nts designed be controlled from a PC To preserve the integrity of the equipment it is essential that the attached PC itself conforms to basic safety and EMC standards and is set up in accordance with the manufacturers instructions If in doubt consult the N information that came with your PC In common with all computer operation the following safety precautions are advised WARNING To reduce the chance of eye strain set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings e To reduce the chance of physical strain set up the PC display keyboard and mouse with correct ergonomic positioning according to your local safety guidelines Class A equipment is intended for use in an industrial environment This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference N when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if WARNING not installed and used in accordance with these instructions may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense
16. resentative En repr sentation du fabricant Carme Canal s General Manager Panlab s l u a division of Harvard BioScience Cornell de Llobregat Spain 30 04 2014 Automated black amp white test box 22 n Harvard z Apparatus Note on environmental protection After the implementation of the European Directive 2002 96 EU in the national legal system the following applies Electrical and electronic devices may not be disposed of with domestic waste Consumers are obliged by law to return electrical and electronic devices at the end of their service lives to the public collecting points set up for this purpose or point of sale Details to this are defined by the national law of the respective country This symbol on the product the instruction manual or the package indicates that a product is subject to these regulations By recycling reusing the materials or other forms of utilising old devices you are making an important contribution to protecting our environment taf Nota sobre la protecci n medioambiental Despu s de la puesta en marcha de la directiva Europea 2002 96 EU en el sistema legislativo nacional Se aplicara lo siguiente Los aparatos el ctricos y electr nicos as como pilas y bater as no se deben tirar ala basura dom stica El usuario est legalmente obligado a llevar los aparatos el ctricos y electr nicos as como pilas y bater as al final de su vida til a los puntos de
17. s aparelhos el ctricos e electr nicos n o podem ser despejados juntamente com o lixo dom stico Consumidores est o obrigados por lei a colocar os aparelhos el ctricos e electr nicos sem uso em locais p blicos especficos para este efeito ou no ponto de venda Os detalhes para este processo s o definidos por lei pelos respectivos pa ses Este s mbolo no produto o manual de instru es ou a embalagem indicam que o produto est sujeito a estes regulamentos Reciclando reutilizando os materiais dos seus velhos aparelhos esta a fazer uma enorme contribuic o para a protecc o do ambiente D Automated black amp white test box 23
18. tem is ready to work the ready led stays on continuously e WHITE BULB This switch is used to turn the white bulb in the white compartment on and off e ANIMAL POSITION These 2 red leds indicate the animal position The animal position is detected through load cells e RED BULB This switch turns the red bulb in the black compartment on and off Automated black amp white test box 10 Harvard Pa n la b Apparatus 7 2 CONTROL UNIT REAR PANEL BOX ID BULBS RAT MOUSE POWER Bdx Id Main Remote Made in Spkin by Panlab s l Doooooooooooo Rated Voltage 90 260 V AC 50 60 Hz 18VA MAIN REMOTE BOX Figure 7 Control Unit Rear Panel e BOX ID Decimal selector used to identify the cage When several cages are connected to the same computer no 2 cages can have the same Id number e MAIN female connector used to connect the control unit to the serial port of the computer When the computer controls several control units connected in cascade the serial port of the computer is connected to the MAIN port of the first control unit and the MAIN port of next unit is connected to the REMOTE port of the previous control unit The REMOTE port of the last unit is left free e REMOTE DB9 male connector used to connect the control unit to the MAIN port of the next control unit when several cages are controlled by a computer The REMOTE port of the last unit is left
19. tronische Ger te am Ende ihrer Lebensdauer an den daf r eingerichteten ffentlichen Sammelstellen oder an die Verkaufstelle zur ckzugeben Einzelheiten dazu regelt das jeweilige Landesrecht Das Symbol auf dem Produkt der Gebrauchsanleitung oder der Verpackung weist auf diese Bestimmungen hin Mit der Wiederverwertung der stofflichen Verwertung oder anderer Formen der Verwertung von Altger ten leisten Sie einen wichtigen Beitrag zum Schutz unserer Umwelt PE Informazioni per protezione ambientale Dopo l implementazione della Direttiva Europea 2002 96 EU nel sistema legale nazionale ci sono le seguenti applicazioni dispositivi elettrici ed elettronici non devono essere considerati rifiuti domestici consumatori sono obbligati dalla legge a restituire dispositivi elettrici ed elettronici alla fine della loro vita utile ai punti di raccolta collerici preposti per questo scopo o nei punti vendita Dettagli di quanto riportato sono definiti dalle leggi nazionali di ogni stato Questo simbolo sul prodotto sul manuale d istruzioni o sull imballo indicano che questo prodotto soggetto a queste regole Dal riciclo e re utilizzo del material o altre forme di utilizzo di dispositivi obsoleti voi renderete un importante contributo alla protezione dell ambiente I lt Nota em Ambiental Ap s a implementac o da directiva comunit ria 2002 96 EU no sistema legal nacional o seguinte aplica se Todos o
20. ty you can wet the cloth with a soapy solution to clean them then remove foam with a wet cloth and finally dry them with a dry cloth Automated black amp white test box 17 11 TROUBLESHOOTING Panlab pb This table features instructions to solve the most frequent problems PROBLEM The equipment does not start up The two red position leds are blinking One of the red position leds is blinking The light stimuli do not work The control unit does not detect animal position The equipment does not communicate with PPCWin Automated black amp white test box SOLUTION Ensure that the voltage of mains is the same as that selected in the fuse holder Check the condition of the fuses Ensure that the cable DB25 to DIN7 connects the cage and control unit Check that the floors are correctly placed If the cable is connected and all is correctly placed this means both load cells are damaged The load cell is damaged or blocked Check that 4 pins cable is connected Check that DB25 to DIN7 cable is connected Check that Ready led is on Check that bulbs are not melt Check that Ready led is on Check that floors are correctly placed Check that the RAT MOUSE switch is in the correct position Rat weight threshold detection is 3ogr if you work with mice the equipment will not be able to detect them Make sure your equipment is connected to PC via RS 232 Check that PPCWin settings so that th
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