Review of Contractor Data
Contents
1. The remaining categories shall be filled out as follows Specimen Category no export Analysis Method G5_Advanced or NCSBI Robot for Robot runs Panel Identifiler_v2 Size Standard GSSOOLIZ_Advanced Instrument Type ABI3130 or similar note automatically filled in by the software and instrument Once all samples and categories are properly labeled and identified select the Analyze icon to analyze the data When prompted create a project name for the set of samples and select Save 5 2 7 1 Note GMID auto saves data after every 10 samples have been processed 5 2 7 2 Note The Genotypes tab becomes available in the main GMID window once analysis is complete 5 2 7 3 Note If any sample displays a solid red stop sign icon on the right hand side of the GMID window assess the data as described in 5 3 Analysis and Plot Settings 5 2 8 1 Access the Analysis Method Editor by selecting Analysis from the menu in the main GMID window and then select Analysis Method Editor Note that the user must have a single sample in the main GMID window selected in order to access the Editor 5 2 8 2 Select the Peak Detector tab and ensure that the settings are as seen below when the samples have been amplified with Identifiler Note that the Peak Page 3 of 14 All copies of this document are uncontrolled when printed Procedure for GeneMapper ID for DNA Database Version 1 Forens2. fsa files associated with such samples controls ladders shall be present in the run folder containing the electronic data in the FA case record object repository or equivalent folder on the server if analysis is not part of a case record 5 3 2 3 2 2 Note For any sample control ladder that is not used for comparison purposes corrections labeling of artifacts are not required If an entire run or project is not used for comparison purposes it shall be printed as Print2PDF and added to the case record object repository or equivalent folder on the server if analysis is not part of a case record Corrections i e labeling of artifacts are not required in this instance A Casework Table shall be generated for this instance and the reason for not using the entire run project shall be noted on this page also Print2PDF see 5 4 1 An Allele Call Table is not required for such runs projects The LIZ sizing standard shall be printed Print2PDF as a separate set of egrams which may be added to the overall egram PDF file for each project or kept separately as an independent document The LIZ must be present for all samples controls ladders and all peaks 75 through 450 must be visible 5 3 3 1 In order to verify the addition of amplification kit primers to all negative controls and negative samples which includes instances of LIZ or injection failure the raw data for such samples from each project must be present in each case record o
3. loci in question If one locus exhibits homozygosity and the second heterozygosity the off ladder allele shall be assigned to the homozygous locus If both loci exhibit homozygosity or heterozygosity the DNA Database Forensic Scientist shall document either on the allele call table or the electropherograms that the allele was observed but not assign the allele to a particular locus and mark the allele as inconclusive INC The called non off ladder alleles at these loci shall be reported and uploaded into CODIS When a locus contains three alleles one of which is off ladder and the adjacent locus is homozygous the DNA Database Forensic Scientist shall call the off ladder allele inconclusive This will not preclude the scientist from reporting and uploading the remaining on ladder alleles to CODIS 5 3 2 2 1 3 2 If the off ladder allele can be assigned to a locus the allele must be designated as either greater than gt or less than lt the last respective allele for that locus called by the allelic ladder For CODIS eligibility refer to the Forensic Biology Section Procedure for CODIS 5 3 2 2 1 3 3 Follow the steps outlined for microvariants for assigning a value to the off ladder allele as well as the printing format with the exception that the comment shall be off ladder allele instead of microvariant Page 9 of 14 All copies of this document are uncontrolled when printed Procedure f
4. to select all samples at the 250 bp peak The sizing table then displays the 250 bp peak size for all selected samples If any sample has a 250 bp peak which is greater than 0 5 bp from the 250 bp peak in the associated allelic ladder refer to 5 3 1 2 1 If any sample or control in the project indicates no sizing data i e LIZ failure then that sample or control shall not be used for comparison and shall be re injected to achieve proper sizing information This information shall be noted on the Casework Table Worksheet If there is no sizing data for an allelic ladder then no data associated with that ladder can be sized and the entire set shall be re injected Assess Overall Quality of Data 5 3 2 1 Allelic Ladders and Controls 5 3 2 1 1 In the main GMID window select the allelic ladder s and open the sample plot window by selecting the Display Plots icon In the Samples Plot window ensure that the Plot Setting is set to AmpFLSTR Genotyping Page 6 of 14 All copies of this document are uncontrolled when printed Procedure for GeneMapper ID for DNA Database Version Forensic Biology Section Effective Date 09 13 2013 5 3 2 1 2 Scroll through each of the color channels blue green yellow and red and verify that all the alleles are sized and labeled correctly according to the expected calls for the Identifiler Allelic ladder All expected peaks must be present and above the appropriate analytic
5. 100 139 150 160 200 300 340 350 400 and 450 base pairs Close this window 5 3 1 1 1 Note If all peaks are not called the analysis range may need to be extended view the Analysis Settings in 5 2 8 2 and change the analysis range to Full Range in the Peak Detector tab Again select all the samples in the main GMID window and select the Display Plots icon W This opens the Sample Plot window for all selected samples Change the Plot Setting to Overlay LIZ by selecting it from the dropdown menu in the top left portion of the Sample Plot window Zoom in to the area near the 250 base pair peak bp by holding the mouse just above the horizontal scale indicating bp size and then clicking and dragging to zoom 5 3 1 2 1 Note the 250 bp peak was not defined by the manufacturer in the size standard The purpose of checking this sizing peak is to determine if the 250 bp peak within the samples and controls are within 0 5 bp of the 250 bp sizing peak of the allelic ladder they were injected with Therefore no sample or control run in house shall be used for analysis if its 250 bp peak falls outside of this window in relation to the allelic ladder Any sample or control that exceeds the window shall be re injected refer to the Forensic Biology Section Procedure for Use of the 3130XL Genetic Analyzer for reinjection requirements Highlight the 250 bp peak and hold down the Ctrl and S key on the keyboard
6. merged with the set up sheet Page 12 of 14 All copies of this document are uncontrolled when printed Procedure for GeneMapper ID for DNA Database Version Forensic Biology Section 6 0 5 4 2 5 4 3 Effective Date 09 13 2013 5 4 1 9 Note For CODIS confirmations only samples and controls specific to the CODIS confirmation shall be present in the Casework Table Allele Call Table 5 4 2 1 In the main GMID window select the Case Allele Table from the Table 5 4 2 2 5 4 2 3 5 4 2 4 5 4 2 5 5 4 2 6 5 4 2 7 5 4 2 8 Setting menu With all samples selected select the Genotypes tab in the main GMID menu Under the File menu in the main GMID window select Export Table Save the table to a location easily located by the DNA Database Forensic Scientist on his her computer i e desktop or thumb drive Ensure the file is exported saved in the Tab and Comma delimited Text txt format 5 4 2 2 1 Note the DNA Database Forensic Scientist may need to add additional information to the txt file name in order to avoid copying over the Casework Table file Open Excel open the txt file saved in the previous step note the DNA Database Forensic Scientist may have to change the files of type to All Files in order for the software to access the txt file When the Text Import Wizard opens select Finish Open the Allele Call Table Excel file in the Forensic Bi
7. use of overblown samples in Database samples shall be at the discretion of the DNA Database Forensic Scientist based upon the training and experience of the DNA Database Forensic Scientist 5 3 2 2 1 6 Tri alleles The sample shall be re extracted except in CODIS confirmations 5 3 2 3 Printing Egrams Electronically 5 3 2 3 1 All egrams shall be printed using the Print2PDF printer with the exception of 5 3 2 2 1 3 5 The egrams for each GMID project shall exist as its own PDF file 5 3 2 3 1 1 Note GMID projects are added to the FA case record object repository or equivalent folder on the server if analysis is not part of a case record by exporting the Page 10 of 14 All copies of this document are uncontrolled when printed Procedure for GeneMapper ID for DNA Database Version Forensic Biology Section 5 3 2 3 2 5 3 2 3 3 5 3 2 3 4 5 3 3 View Raw Data Effective Date 09 13 2013 projects in GMID select Tools GeneMapper Manager select the applicable project and select export The file is exported saved as an ser file Any individual sample control ladder that is not used for comparison purposes i e LIZ failure redundancy further analysis shall be printed in PDF format Such samples controls ladders shall appear in the Casework Table explaining it was not used for comparison e g LIZ failure redundancy re analysis 5 3 2 3 2 1 Note Even if not used for comparison purposes all
8. 11 D2S1338 D19S433 and Amelogenin Injection See Forensic Biology Section Autosomal DNA STR Interpretation Procedure LIZ Sizing Standard A commercially produced set of DNA fragments of known size used to determine the size of DNA fragments in an electrophoresed sample Locus plural loci See Forensic Biology Section Autosomal DNA STR Interpretation Procedure also referred to as a marker Microvariant See Forensic Biology Section Autosomal DNA STR Interpretation Procedure Off Ladder Allele See Forensic Biology Section Autosomal DNA STR Interpretation Procedure Overblown Off Scale Data See Forensic Biology Section Autosomal DNA STR Interpretation Procedure Panel A collection of markers specific to an amplification kit 1 e Identifiler Project A set of data saved as a single entity in GMID Relative Fluorescence Units RFUs A unit of measurement used in electrophoresis when fluorescence detection is utilized determines peak height Run See Forensic Biology Section Autosomal DNA STR Interpretation Procedure Split Peaks See Forensic Biology Section Autosomal DNA STR Interpretation Procedure Equipment and Materials Computer with GeneMapper ID version 3 2 1 or most current software Microsoft Excel Word Print2PDF software Procedure Page 1 of 14 All copies of this document are uncontrolled when printed Procedure for GeneMapper ID for DNA Database Version 1 Forensic Biology Section Effecti
9. 6 5 4 1 7 5 4 1 8 In the main GMID window select the SBI Casework Table from the Table Setting menu Select all samples and under File in the main GMID menu select Export Table Save the table to a location easily located by the DNA Database Forensic Scientist i e desktop or thumb drive Ensure the file is exported saved in the Tab and Comma delimited Text txt format Open Excel open the txt file saved in the previous step note the DNA Database Forensic Scientist may have to change the files of type to All Files in order for the software to access the txt file When the Text Import Wizard opens select Finish Adjust the columns to accommodate the full text for each line Select Page Setup Under the Page tab select landscape under the Header Footer tab select Custom Header at a minimum the DNA Database Forensic Scientist shall enter the batch number initials and the date under the Sheet tab select only the Gridlines box Finish by selecting OK DNA Database Forensic Scientists shall note on this page any samples that are not to be used for comparison and why or those that are to have additional analysis performed on them see 5 3 2 3 2 Print the Casework Table Worksheet by using the Print2PDF printer The Casework Table Worksheet shall exist independently of the egrams and the Allele Call Table Worksheet s It may be
10. 8775 Rev C Applied Biosystems AmpRSTR Identifiler PCR Amplification Kit User s Manual P N 4323291 Rev B Forensic Biology Section Procedure for DNA Database Autosomal DNA STR Interpretation Forensic Biology Section Procedure for DNA Database Use of the 3130XL Genetic Analyzer Forensic Biology Section Procedure for CODIS Forensic Biology Section DNA Database Procedures Forensic Biology Section Procedure for DNA Database Review of Contractor Data Forensic Biology Section Procedure for DNA Database Analysis and Technical Review of Database Samples NDIS STR Data Entry Software accessible by registered CODIS users only Records e Identifiler Allelic Ladder Expected Results e Identifiler 9947A and MJB Expected Results Attachments N A Revision History 09 13 2013 Original Document Page 14 of 14 All copies of this document are uncontrolled when printed
11. ID window select the allelic ladder followed by the sample s containing the microvariant Display Plots Zoom in to the locus containing the microvariant the Sample Plot displays the allelic ladder in the top half of the Plot window and the sample is in the bottom half 5 3 2 2 1 2 2 Highlight the microvariant in the sample along with the alleles in the allelic ladder that lie the closest in size to the microvariant to Page 7 of 14 All copies of this document are uncontrolled when printed Procedure for GeneMapper ID for DNA Database Forensic Biology Section Version 1 Effective Date 09 13 2013 select peaks simultaneously hold down the Ctrl key on the keyboard while selecting Select the sizing table icon ke Select Ctrl S to make the sizing table display the selected alleles 5 3 2 2 1 2 3 Based upon the sizing information provided in the table determine the appropriate allele call for the microvariant For CODIS eligibility refer to the Procedure for CODIS 5 3 2 2 1 2 3 1 Note the correct allele call for the microvariant is dependent upon the microvariant s base pair size when compared to the allelic ladder 5 3 2 2 1 2 3 2 Note microvariants that vary by less than the consensus repeat unit for that locus must be designated as an integer of that variation i e the 9 3 allele at THO1 5 3 2 2 1 2 4 Rename the allele by right clicking on it and selecting Rename Allele If the correct
12. Procedure for GeneMapper ID for DNA Database Version Forensic Biology Section Effective Date 09 13 2013 1 0 2 0 3 0 4 0 5 0 Procedure for GeneMapper ID for DNA Database Purpose This procedure specifies the steps for performing analysis on DNA samples using GeneMapper ID GMID software Scope This procedure applies to DNA Database Forensic Scientists in the Forensic Biology Section who perform DNA analysis using GMID Definitions Amelogenin Gender determining short tandem repeat STR locus Artifact See Forensic Biology Section DNA Database Autosomal DNA STR Interpretation Procedure Bin An expected location for a particular allele within a locus a binset is a collection of expected locations for alleles at loci amplified as a set Consensus Allele An allele that conforms to an incremental repeat pattern at an STR locus Electropherogram egram The computer generated electronic visual result from an analysis performed by electrophoresis fsa File A raw data file generated during sample electrophoresis as part of a run only viewable through GMID software or equivalent Heterozygote Peak Imbalance A difference of greater than approximately 50 between two peaks of a single source sample at a given locus Identifiler A commercially produced amplification kit which contains the following STRs CSF1PO FGA THO1 TPOX vWA D3S1358 D5S818 D7S820 D8S1179 D13S317 D16S539 D18S51 D21S
13. al threshold Close this Sample Plot window 5 3 2 1 3 In the main GMID window select the positive and negative amplification and extraction controls if used Open the sample plot window as described previously 5 3 2 1 4 Examine the positive control s for the expected allelic results and to ensure that the negative controls meet the required interpretive guidelines see the Forensic Biology Section Procedure for DNA Database Autosomal DNA STR Interpretation Remove any artifacts as described in 5 3 2 2 1 1 Close the Sample Plot window 5 3 2 2 Samples 5 3 2 2 1 Examine all samples in the manner as described for the allelic ladders and controls 5 3 2 2 1 1 Artifacts If any peaks are to be removed from consideration as true alleles due their presence as a known artifact i e pull up dye blob stutter spike highlight the sizing box associated with the peak and right click the mouse Select Delete Allele s multiple peaks can be deleted simultaneously by selecting them at the same time when the warning dialog box appears select OK When prompted by the Delete Allele Comment type in artifact and the DNA Database Forensic Scientist s initials 5 3 2 2 1 2 Microvariants If a microvariant is present in a sample and GMID has not automatically assigned it an allelic value DNA Database Forensic Scientists shall document the proper sizing of the microvariant as follows 5 3 2 2 1 2 1 In the main GM
14. allele call is not already present in the subsequent drop down list select Custom and enter the call in the Create Custom Allele Name box When prompted by the Edit Allele Comment box type in microvariant and the DNA Database Forensic Scientist s initials 5 3 2 2 1 2 5 With the alleles highlighted in 5 3 2 2 1 2 2 still present in the sizing table remove any extraneous select File and Print and select Print2PDF 5 3 2 2 1 2 6 If a microvariant is observed re amplification or re extraction of the sample shall be at the DNA Database Forensic Scientist s discretion see the Forensic Biology Section DNA Database procedures 5 3 2 2 1 3 Off Ladder Alleles If a sample s contains an off ladder allele that is not an artifact the DNA Database Forensic Page 8 of 14 All copies of this document are uncontrolled when printed Procedure for GeneMapper ID for DNA Database Forensic Biology Section Version 1 Effective Date 09 13 2013 Scientist shall assign the allele to the correct locus if possible see below For CODIS eligibility refer to the Forensic Biology Section Procedure for CODIS 5 3 2 2 1 3 1 1 5 3 2 2 1 3 1 2 5 3 2 2 1 3 1 3 5 3 2 2 1 3 1 4 If the allele in question lies between two loci the DNA Database Forensic Scientist shall attempt to determine which locus the allele should be assigned to based upon the presence or absence of heterozygosity at the
15. bject repository or equivalent folder on the server if analysis is not part of a case record 5 3 3 1 1 In the main GMID window highlight the negative control or negative sample and select Raw Data from the View option in the main GMID menu Page 11 of 14 All copies of this document are uncontrolled when printed Procedure for GeneMapper ID for DNA Database Version Forensic Biology Section Effective Date 09 13 2013 5 3 3 1 2 The subsequent window displays the raw data on the right hand side and the sample and run information on the left hand side Expand the left hand window so that the full run file name including run number is clearly visible The right hand window shall show the maximum vertical and horizontal axis 5 3 3 1 3 Using the Alt Print Screen buttons on the computer take a snapshot of the raw data window Open up a Word document and paste this snapshot into the document Repeat this process for all negative samples and negative controls in each project 5 3 3 1 3 1 This raw data Word document header shall contain at least the project name refer to DNA Database Procedure DNA Database Forensic Scientist s initials and the date 5 3 3 1 4 The Word document s containing the snapshots of the raw data shall be included in the scanned or Print2PDF notes 5 4 Exporting and Creating Tables 5 4 1 Casework Table 5 4 1 1 5 4 1 2 5 4 1 3 5 4 1 4 5 4 1 5 5 4 1
16. current project follow 5 2 3 through 5 2 8 The newly added sample shall be analyzed select the Analyze icon before the data can be viewed 5 2 9 1 1 Note if the sample is from a different injection i e run folder the associated Allelic ladder shall also be imported in order for the sample data to be sized properly 5 2 9 2 If a DNA Database Forensic Scientist must remove a sample s that has been added to the project i e a sample from an unrelated CODIS confirmation highlight the sample to be removed and in the menu bar select Edit and Delete from Project Select OK when prompted 5 2 9 2 1 Note samples from the samein house analysis or confirmation that are present in a project but are not used i e redundancy sizing data issues or similar shall not be deleted refer to 5 3 2 3 2 5 3 Analyzing Preliminary Evaluation of Data of Each GMID Project 5 3 1 Evaluate Sizing Data 1 e LIZ 5 3 1 1 Select Edit from the GMID menu bar at the top of the window and Select All Click on the Size Match Editor icon The new window that opens Page 5 of 14 All copies of this document are uncontrolled when printed Procedure for GeneMapper ID for DNA Database Version 1 Forensic Biology Section 5 3 2 5 3 1 2 5 3 1 3 5 3 1 4 Effective Date 09 13 2013 displays the LIZ sizing data for all selected samples scroll through each one and confirm that all sizing peaks are correct 75
17. ic Biology Section Effective Date 09 13 2013 Threshold is set to 100 for the blue green yellow and red channels Note that the threshold for orange LIZ is set to 80 RFU for all Database samples Analysis Method Editor HID General Allele Peak Quality Quality Flags Peak Detection Algorithm Advanced v Ranges Peak Detection Analysis Sizing Peak Amplitude Thresholds Partial Sizes Y B 100 R 100 Start Pt 2500 Start Size 70 Stop Pt 8800 Stop Size 500 G 100 0 80 Y 100 Smoothing and Baselining Smoothing O None Light O Heavy Peak Window Size Slope Threshold Peak Start Peak End Min Peak Half Yvidth Polynomial Degree Baseline Window 51 pts Size Calling Method 2nd Order Least Squares 3rd Order Least Squares Cubic Spline Interpolation Local Southern Method Global Southern Method Factory Defaults For Database robot samples the Peak Threshold for the blue green yellow and red channels shall be set at a value between 100 and 175 RFUs 5 2 8 3 Next select the Allele tab the contents should appear as below Analysis Method Editor HID General Allele Peak Detector Peak Quality Quality Flags Bin Set AmpFLSTR_Bins_v2 Use marker specific stutter ratio if available Marker Repeat Type Tri Tetra Cut off Value 02 0 0 Minus amp Ratio oo o0 Minus Distance oo o
18. o To 00 0 0 Minus Stutter Ratio 0 0 Minus Stutter Distance From E E E 325 To 3 475 Plus Stutter Ratio oo joo Plus Stutter Distance From i 0 0 To J 0 0 Amelogenin Cutoff o0 Range Filter Page 4 of 14 All copies of this document are uncontrolled when printed Procedure for GeneMapper ID for DNA Database Version 1 Forensic Biology Section Effective Date 09 13 2013 5 2 8 4 In the main GMID window select Tools from the top menu and then select Table Setting Editor Under the Genotypes tab at the bottom of the window ensure that the Number of Alleles under Allele Settings is set to at least 12 DNA Database Forensic Scientists may adjust this number if necessary i e to maximum number of called allele at any locus Table Setting Editor General Samples Genotypes Genotypes Table Settings Column Settings Font Settings Column Filtering Font Arial 1 Sample File Show All Records Size 1 Sample Name Show All Records Sample ID Show All Records Run Name Show All Records Panel Show All Records Allele Settings Number of Alleles 12 C Keep Allele Size Height Area Data Point Mutation and Comment together 5 2 9 Adding Deleting samples 5 2 9 1 If a DNA Database Forensic Scientist must add an additional sample s to a
19. ology Shared Folder For Robot runs use the Robot Allele Call Table For manual runs performed in the database either the Robot Allele Call table or manual allele call table may be used Copy and paste the allele calls generated from the txt file in 5 4 2 4 Select Al into the Results tab in the appropriate Allele Call Table Worksheet Return to the Allele Call Table tab or the Mixture tabs if any samples have more than 2 alleles at any given locus and verify that the copy and paste function worked properly Print the Allele Call Table Worksheet s using the Print2PDF printer This file must be separate from the file containing the egrams or the Casework Table Note For CODIS confirmations only samples specific to the CODIS confirmation shall be present in the Allele Call Table CODIS Table 5 4 3 1 5 4 3 2 Refer to the Procedure for CODIS Procedure for DNA Database Review Contractor Data and the Procedure for DNA Analysis and Technical Review of Database Samples for CODIS sample export and specimen upload Limitations GMID cannot be used on computers operating on Windows 7 or higher Page 13 of 14 All copies of this document are uncontrolled when printed Procedure for GeneMapper ID for DNA Database Version Forensic Biology Section Effective Date 09 13 2013 7 0 Safety N A 8 0 References 9 0 10 0 Applied Biosystems GeneMapper ID Software Version 3 1 User s Guide P N 433
20. or GeneMapper ID for DNA Database Version Forensic Biology Section Effective Date 09 13 2013 5 3 2 2 1 3 4 If an off ladder allele occurs re amplification or re extraction shall be at the DNA Database Forensic Scientist s discretion see 5 3 2 2 1 2 6 5 3 2 2 1 3 5 If the GMID software does not allow the DNA Database Forensic Scientist to assign a value to the off ladder allele the DNA Database Forensic Scientist shall follow the same procedure for documentation and assignment of microvariants The DNA Database Forensic Scientist shall be required to print out the sizing table egram combination and manually write the proper sizing designation to the off ladder allele along with the DNA Database Forensic Scientist s initials These pages shall then be scanned into PDF format 5 3 2 2 1 3 5 1 Note the Allele Call Table Worksheet also needs to be changed to reflect the proper allele call for the off ladder allele that cannot be exported through the GMID software 5 3 2 2 1 4 Sizing Shifts If any allele at any locus has been sized outside of the appropriate bin which results in an off ladder call by GMID the sample shall be re injected or re amplified if necessary in order to size the allele s properly if the DNA Database Forensic Scientist wants to use the results at that locus Shifted alleles loci shall not be used for comparison in any control ladder or sample 5 3 2 2 1 5 Overblown Off scale Samples The
21. s_NC_v2_Panels txt and AmpFLSTR_Panels_NC v2_AmpFLSTR_Bins_NC_v2 bins txt Refer to DNA Database Review of Contractor Data for review procedure 5 2 3 Select File and Add samples to project Page 2 of 14 All copies of this document are uncontrolled when printed Procedure for GeneMapper ID for DNA Database Version 1 Forensic Biology Section 5 2 4 5 2 5 5 2 6 5 2 7 5 2 8 Effective Date 09 13 2013 Locate the run folder containing the samples to be added to the project under the Files tab Highlight the run folder or select the specific samples to be analyzed and select Add to List The file s then appears on the right hand side Select Add In the Samples pane the category Sample Type shall have the samples labeled as follows when amplified with Identifiler Ladder Allelic Ladder 9947A Positive Amplification Control Positive Control Negative Amplification Control Negative Control All other samples shall be Samples Note Sample File names appear by well number i e A12 followed by the sample name as entered by the DNA Database Forensic Scientist prior to electrophoresis Also until the samples are analyzed the Status icon to the left of the Sample File name has the unanalyzed icon i present next to it If no Status category or icons are present verify that the table setting in top center of the main GMID window is set to AmpFLSTR Table
22. ve Date 09 13 2013 5 1 Setting Analysis Parameters for GMID Each user shall import from the Section Shared Drive the following files as required for the type of analysis being performed into his her copy of GMID prior to analysis if not already present 5 1 1 Analysis Methods e G5_Advanced xml e NCSBI Robot xml Database Robot runs only e North Carolina v2 xml Database Contractor Data Review only provided by contract laboratory 5 1 2 Panels Bins e AmpFLSTR_Panels_v2 txt e AmpFLSTR_Panels_v2_AmpFLSTR_Bins_v2_bins txt e AmpFLSTR_Panels_NC_v2_Panels txt Database Contractor Data Review only provided by contract laboratory e AmpFLSTR_Panels_NC_v2_AmpFLSTR_Bins_NC_v2_bins txt Database Contractor Data Review only provided by contract laboratory 5 1 3 Size Standards e GSS500LIZ_Advanced xml 5 1 4 Tables and Plot Settings SBI Casework Table xml AmpFLSTR Sizing plot xml Case Allele Table xml Overlay LIZ plot xml AmpFLSTR Genotyping plot xml AmpFLSTR Table xml CODIS Export Table xml 5 2 Creating a Project 5 2 1 Open GMID and log in under DNA Database Forensic Scientist s name log in Analysis under the generic gmid log in name is not permitted 5 2 2 For Database review of contractor data the contractor provided ser file s shall be imported using GeneMapper Manager The following files must be added to GMID prior to review e Analysis Method North Carolina v2 xml e Panels Bins AmpFLSTR_Panel
Download Pdf Manuals
Related Search