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The Genevac EZ-Bio Evaporation System User Manual

1. General Information This manual has been produced to assist in the daily running and routine maintenance of the Genevac Evaporator Amendment Control Form Only the effected pages and the Front Page will display the Revision Number all others will stay at initial issue Revision Issue and Reason for Change Date Issued Number Only 1 Tip n Tell indicator now fitted to Packaging March 2005 Update to Solvent Group Guide Section 1 Chapter 3 Page 1 July 2005 These instructions are subject to change without notice No part of these instructions may be reproduced in any form or be processed duplicated or distributed by electronic or optical means without the written permission of Genevac Limited All rights reserved Genevac Limited These operating instructions should be read before you use the Genevac EZ Bio Evaporating System Keep them near the system for easy reference Your attention is drawn in particular to Page vi Paragraph 3 Safety 10 1610 Issue 1 2 July 2005 CONTENTS Amendment Control Form 1 Introduction 2 Safety and Maintenance Notes WARNING Caution Note Genevac Evaporators and Combustible Solvents 3 SAFETY 3 1 Safe loading of rotor 3 2 Lid operation 3 3 Limitations of use SECTION 1 GENERAL Chapter 1 UNPACKING AND SETTING UP Flight Case Consumer Packaging Chapter2 QUICK START Quick Start Guide Error Warnings Chapter3 DETAILED OPERATION SOLVENT LIMI
2. 8 Warranty does not cover accidental damage misuse or inappropriate repair by untrained personnel Failure to adhere to the above would result in the costs of repairs being charged Unit supplied in reusable packaging This is to be retained for reuse by client in the unlikely event that system needs to be returned for repair If package not retained a charge will be made for replacement packaging and shipping costs incurred Section 4 Page 3 Genevac EZ Bio Evaporating System User Manual Genevac Limited The Sovereign Centre Farthing Road Ipswich IP1 5AP United Kingdom Sales and Service Hotlines Service Hotline 44 0 1473 243000 Sales Hotline 44 0 1473 240000 Fax 44 0 1473 461176 Email salesinfo genevac co uk Web site http www genevac com Genevac Inc 707 Executive Boulevard Suite D Valley Cottage New York 10989 United States of America Sales and Service Hotline 1 845 267 2211 Fax 1 845 267 2212 Email salesinfo genevacusa com Section 4 Page 4 Useful information If you need to contact Genevac for assistance use either the telephone or fax Hotlines given It will always help Genevac Service if you have the serial numbers at hand for the components of your system If you need to contact Genevac Sales for information on Service Contracts or products use the telephone or fax Hotlines given Alternatively Email or visit our web site 10 1610 Issue 1 January 20
3. 9 2 5 3 6 1 7 1 8 1 Preparation Ensure that evaporator is switched OFF Remove blanking cap from connector on evaporator Plug software Data Key into connector Uploading Switch on evaporator The evaporator will automatically load software The existing code has to be deleted first The display will show ErS bLc n where is the block number Each block could take up to 8 seconds and there are 4 blocks to erase There will be no progress indication during each block erase cycle The block number will increment as each block has been erased The software will then load code data from key storing at the appropriate destination with the Hour Glass symbol animated in the top right hand corner of the LCD display During upload the system displays LoAd codE This process can take up to 34 seconds so the time remaining target at the lower right hand corner of the display will illuminate showing a percentage countdown 99 0 When the rSt SYS message is displayed 5 3 1 Switch off 5 3 2 Remove the software Data Key 9 3 9 Refit blanking cap onto connector 5 3 4 Switch back on the new code is then used by the system Testing No formal testing is required Errors If any errors are observed and or the evaporator fails to operate make a note of the error code s displayed and contact Genevac service Completion Place software Data Key in a safe and secure place Sectio
4. Genevac EZ Bio Evaporating System Owners Manual 1 Introduction This chapter provides guidance in the removal replacement and refitting of Fuses 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipment Quantity 100 Volt 50Hz System AA F1 5 04 F2 5 04 F3 8 04 F4 2 04 F5 10 04 F6 Main Supply 16 0A F7 Main Supply 16 0A 100 Volt 60Hz System F1 5 0A 04 3595 F2 5 0A 04 3595 F3 8 0A 04 3592 F4 2 0A 04 3594 F6 Main Supply 16 0A F7 Main Supply 16 0A SR 1 1 120 Volt System EEE 1 GERNE F2 6 3A F3 8 0A F4 3 15A F5 10 0A F6 Main Supply 10 0A F7 Main Supply 10 0A 230 240 Volt System F1 4 04 F2 3 15A F3 4 0A F4 2 0A F5 5 0A F6 Main Supply 6 3A F7 Main Supply 6 3A N ENSURE THAT EVAPORATOR IS ISOLATED FROM MAINS POWER SUPPLY N ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS 10 1610 Issue 1 January 2005 Section 3 Chapter 3 Page 1 Genevac EZ Bio Evaporating System User Manual Caution Refer to manual before replacing fuses Vent Inlet Caution Refer to manual before replacing fuses Attention se reporter au manuel d utilisation avant de changer les fusibles Vorsicht lessen Sie bitte das
5. Setting Run Time The maximum run time can be changed during a run should the need be to concentrate samples rather than dry them This can achieved in increments of 1 minute going up to hourly increments Press and hold the START button for 5 seconds the pressure indication will change to display SEt which will flash rapidly The Elapsed Time display will now indicate the proposed maximum run time By pressing the START button you can increase the time and by pressing the LID button you can decrease the time Once you have set the required maximum run time release the buttons 5 seconds later the EZ Bio display will revert back to the current run status and the run will continue for the duration that you have just set All run duration changes are saved so that the next time that you select the same run the time that you previously set will be used Section 1 Chapter 3 Page 4 10 1610 Issue 1 January 2005 Genevac EZ Bio Evaporating System User Manual Another feature of the software is the ability to adjust the maximum run time further If the previous procedure has been used then you can also adjust the maximum run time by increments of 10 minutes by momentarily pressing the START or LID buttons If the maximum run time is 2 hours or less then you can only adjust the time by 1 minute increments This adjustment is not saved so will not affect future runs During a run Time Remaining will be incrementing as the run progresse
6. 1 Chapter 3 Page 3 Genevac EZ Bio Evaporating System User Manual Pre cool Stage Pre cooling is automatically selected when required If the chamber is not at or sufficiently close to the required temperature when the start button is pressed then the system will enter pre cool mode Pre cool is sometimes required to avoid bumping of volatile solution If the system enters pre cool mode with the lid closed then the rotor will spin up in pulsed mode The pre cool temperature is determined by the run selected Changing the Run You are able to change the run type at any time even during arun The system will automatically re program the system to the new settings The new run is only accepted after a 3 second thinking time incase you change your mind and re select the original run Acceptance is confirmed by a short beep The state of the Dri Pure LED indicates if Dri Pure is used in the run indicated but will not be illuminated once the run is accepted after the 3 second thinking time Max Sample temperature can be adjusted throughout the run and will be accepted immediately Irrespecitve of run selection elapsed time will not be reset If the new run is a two stage run and the time already elapsed is greater than the 1 stage time then the run continues with the 2 stage settings If the maximum allowable time for the new run is shorter than the time already elapsed then the run will terminate
7. 1610 Issue 1 January 2005 Section 1 Chapter 1 Page 3 Genevac EZ Bio Evaporating System User Manual IN 14 Connect the long exhaust hose supplied and duct into fume hood p DURING ITEM 14 THE EXHAUST HOSE MUST BE FITTED THIS HOSE MUST THEN BE DUCTED TO A SUITABLE EXTRACTION UNIT AREA OR A FUME HOOD 15 Connect suitable drain hose and container not supplied To maintain a solvent vapour free environment around your EZ Bio we suggest that you do not use free space within the hood for storage of vessels containing solvents or acids Users should also ensure that tubing leading to and from the solvent condenser leads solvents and solvent vapours away from the machine in safe manner n particular the exhaust tube must be fitted to the outlet from the condenser and lead away to the laboratory fume extraction system This will ensure that any residual vapour which passes through the condenser and pump does not cause damage to your system Section 1 Chapter 1 Page 4 10 1610 Issue 1 January 2005 EZ Bio Evaporating System Owners Manual Chapter 2 QUICK START 10 1610 Issue 1 January 2005 Section 1 Genevac EZ Bio Evaporating System Owners Manual 1 Switch ON 6 Close lid 2 Press START button and wait for 90 second 7 Set Max temperature as you would on a rotary safety countdown evaporator 3 Press LID button when LED is lit 8 Select RUN type 4 Open lid
8. 4 Diox EtOH EtAc AcOEt Hept Hex MeOH Py TFA THF PhMe H20 NH Water and Acetonitrile MeCN H2O MeOH Note that where there are two programs listed the first will give better solvent recovery while the second may give slightly faster drying Genevac and the ATEX Directive Please note that it remains the responsibility of the user to consider any solvents being evaporated within the context of the ATEX directive The presence of solvents on the list above indicates only that they will not damage the system If further information is required please contact your Sales Representative or visit http www genevac com 10 1610 Issue 1 2 July 2005 Section 1 Chapter 3 Page 1 Genevac EZ Bio Evaporating System User Manual Power Up The LCD See Display is made up of 110 segments and when the system is switched ON it will illuminate for 1 second so that the you have time to observe that there are no segments missing The display will then clear and display Sys Test for a few seconds before displaying the software version Version displayed may be different to this image COUNTDOWN TIMER Please note that the countdown timer counts down from 96 seconds Do not press the START button until the countdown completed This feature ensures that the EZ Bio is in a safe mode for continued operation There could have been a power failure or power fluctuation in the lab that caused the EZ Bio to rese
9. Issue 1 January 2005 EZ Bio Evaporating System Owners Manual Chapter 6 Pump 10 1610 Issue 1 January 2005 Section 3 Genevac EZ Bio Evaporating System Owners Manual 1 Introduction This chapter provides guidance in the removal replacement and refitting of Pump A 4 Head Pump has been used for illustration purposes only the EZ Bio is supplied with a 2 Head Pump 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipment Description Quantity Pump 2 head 100 120 Volt 70 0516 S A R Pump 2 head 230 Volt 70 0517 5 N ENSURE THAT EVAPORATOR IS ISOLATED FROM MAINS POWER SUPPLY N ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS Traces of solvents may be present that could be harmful to your health or lead to material damage There may be a risk to sample integrity if required vacuum is not achieved 10 1610 Issue 1 January 2005 Section 3 Chapter 6 Page 1 EZ Bio Evaporating System User Manual Removal 4 1 Disconect mains power supply 4 2 From rear of evaporator undo the turn fasteners qty 4 that secure pump tray assembly i Do not subject the PTFE hoses and cables to undue strain 4 3 Carefully withdraw p
10. after beep 9 Press START button your run will start using default settings or last run time entered 5 Load your samples in their holders Whilst the system is running the screen will indicate the progress of the run Please see Section 1 Chapter 3 of User Manual for more details 10 1610 Issue 1 January 2005 Section 1 Chapter 2 Page 1 Genevac EZ Bio Evaporating System User Manual ERROR WARNINGS If an error occurs during a run then one of 2 things will happen 1 The run will be terminated This means that the EZ Bio has detected a failure in something that may have an effect on Sample Integrity The physical condition of the evaporator An error code will be displayed when the rotor has stopped spinning and the pressure is at atmosphere 2 The run will continue in a reduced features mode This means that the EZ Bio has detected a failure in something that does not have any effect on Sample Integrity The physical condition of the evaporator An error code will be displayed when the rotor has stopped spinning and the pressure is at atmosphere INDICATION CAUSE OF ERROR RECTIFICATION CLS Lid Lid not closed when run started Ensure lid is closed CLS drn Condenser drain open Close condenser drain PSH Strt Started with Lid or Drain open Push Start button Err Lid Lid Closed indicating Not Closed Operate lid Unlock button mid run Open and re close lid if required Lid Locked indicating Not Locked Operate
11. caps to defective pump Place pump into packaging for despatch back to Genevac Return to Genevac 10 1610 Issue 1 January 2005 Section 3 Chapter 6 Page 3 Genevac EZ Bio Evaporating System Owners Manual Chapter 7 Pump Head Components 10 1610 Issue 1 January 2005 Section 3 Genevac EZ Bio Evaporating System Owners Manual 1 Introduction This chapter provides guidance in the removal replacement and refitting of Pump Head Components 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipment Description 2 Head Pump Head Service Kit Pulley Spanner 44 3 3mm AC2370 1 Allen Key 4mm AB9603 1 Spanner Open Ended 17mm ee pe ENSURE THAT EVAPORATOR IS ISOLATED FROM MAINS POWER SUPPLY ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS Traces of solvents may be present that could be harmful to your health or lead to material damage There may be a risk to sample integrity if required vacuum is not achieved 10 1610 Issue 1 January 2005 Section 3 Chapter 7 Page 1 Genevac EZ Bio Evaporating System User Manual Removal 4 PUMP HEAD ASSEMBLY Fig 1 4 1 Remove pump as detailed in Chapter 6 and place on s
12. damage There may be a risk to sample integrity if required vacuum is not achieved 10 1610 Issue 1 January 2005 Section 3 Chapter 5 Page 1 Genevac EZ Bio Evaporating System User Manual Removal 4 1 Swich evaporator ON wait for 95 seconds as displayed on LCD 4 2 Press LID OPEN button 4 3 Open lid Check for traces of solvent on seal and wipe clean before removing seal NH mum The seal material may absorb significant quantities of some solvents Do not use tools they may damage the chamber 4 4 Manually remove seal from chamber by carefully pulling away from chamber working on a 200mm section at a time Once one comes out the remainder is removed easily 5 Refitting 5 1 Unpack replacement lid seal 5 2 Position on top of chamber wall with lip pointing inwards towards centre 5 3 Work firmly into position using only fingers ensuring the seal is fully home 6 Testing 6 1 Close lid 6 2 Select H2O 6 3 Press START button 6 4 Ensure that pressure drops to below 500 mbar and continues to drop ii When a new seal is fitted manual pressure may be required on the lid to assist to bed the seal If pressure fails to drop check that seal is correctly located on chamber wall and repeat items 6 1 to 6 4 inclusive 6 5 Carry out required run ensure that desired pressure is achieved 7 Completion 7 1 Dispose of defective seal and packaging Section 3 Chapter 5 Page 2 10 1610
13. locate in Strain Washer and rotate clockwise Fig 4 5 6 Carefully refit pump head 5 7 Carefully fit valves qty 2 into pump head Fig 3 5 8 Carefully fit O ring seals qty 2 into pump connecting head Fig 3 5 9 Carefully fit pump connecting head 5 10 Refit pressure plate 5 11 Refit securing screws qty 4 5 12 Reconnect the 2 PTFE tubes to the pump head connections 5 13 Refit pump tray and secure with attachment screws 5 14 Refit pump as detailed in Chapter 6 6 Testing 6 1 Test as detailed in Chapter 6 7 Completion 7 1 Safely discard old seals and valves Section 3 Chapter 7 Page 4 10 1610 Issue 1 January 2005 Genevac EZ Bio Evaporating System Owners Manual Chapter 8 Condenser Drain Valve Knob Assembly 10 1610 Issue 1 January 2005 Section 3 Genevac EZ Bio Evaporating System Owners Manual 1 Introduction This chapter provides guidance in the removal replacement and refitting of Condenser Drain Valve Knob Assembly 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipment Description Quantity Drain Valve Knob Assembly 70 0617 S AAA ENSURE THAT EVAPORATOR IS ISOLATED FROM MAINS POWER SUPPLY ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY AP
14. 05
15. 10 1610 Issue 1 January 2005 THIS EVAPORATOR HAS BEEN DESIGNED TO BE USED IN A POLLUTION DEGREE 1 ENVIRONMENT NO POLLUTION OR ONLY DRY NON CONDUCTIVE POLLUTION ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS Warranty Statement This product is guaranteed for period of 12 months from the date of delivery to site In the unlikely event of any defect arising due to faulty materials or construction resulting in system failure the unit will be repaired free of charge This to include all labour and component costs incurred This warranty is subject to the following provisions 1 System to be returned to Genevac in appropriate packaging for repair 2 System must be sited installed and operated in accordance with operator instruction manual 3 All vapours must be ducted away from the system as described in the installation instructions and operator manual 4 Unit only used for purpose it was sold and in accordance with Genevac published solvent list 5 Preventative maintenance to be adhered to as detailed in operator s manual 6 Consumable items to be replaced as and when necessary by operator Only Genevac approved parts to be used 7 Vacuum pump to be exchanged for refurbished unit in event of failure Owner responsible for exchange and return of failed unit
16. 11 Ni _ gt E 27 E Not Activated Activated Remove clips that retain the lid and remove lid Check contents against packing list Remove the screws from the timber sleeve and carefully lift clear Position base and EZ Bio near to operating bench location With two people using 4 hand holds lift and rotate evaporator to allow access to the back hand holds With assistance 3 people total lift EZ Bio from base and position where required on bench Section 1 Chapter 1 Page 2 10 1610 Issue 1 1 March 2005 Genevac EZ Bio Evaporating System User Manual 10 Ensure that there is 50mm clearance from edge of bench and other equipment The system can be easily moved by placing paper or similar under feet If this is employed ensure all traces are removed on completion 50mm 50mm 2 2 50mm 27 11 Remove accessories containers and check contents against packing note 12 Connect mains supply lead supplied to EZ Bio 13 Remove Condenser Drain Valve transportation blank retain and fit Drain Valve by carefully inserting into condenser and screwing home clockwise until resistance is felt 9 1 AN DO NOT USE THE CONDENSER DRAIN VALVE KNOB TO MOVE OR LIFT THE EZ Bio vr lo FAILURE TO REMOVE BLANK AND FIT CONDENSER DRAIN VALVE KNOB WILL RESULT IN THE EZ Bio DISPLAYING ERROR CLS Drn AND PREVENTING THE RUN FROM STARTING 10
17. 5 kg per swing Do not place any objects on top of the evaporator during a run The following precautions should therefore always be observed Solvents condense in the condenser external catchpot and any exhaust condenser when used These solvents should be disposed of appropriately Solvent vapour is present in the exhaust line this line should be run to a safe extraction source This is particularly important if acids are being used Solvents can condense in the exhaust line and suitable precautions should be taken when working on the EZ Bio This is particularly important if acids have been used Genevac Evaporators and Combustible Solvents Please note it remains the responsibility of the user to consider safety when evaporating any combustible solvents and ensure the system is placed in a well ventilated environment Genevac s position regarding evaporation of such solvents particularly with respect to the European ATEX directive is available on our website or from your local sales representative Page vi 10 1610 Issue 1 January 2005 Genevac EZ Bio Evaporating System User Manual 3 1 Safe loading of rotor Never exceed the maximum load capacity of 1 5 kg per swing Balance sample holders to within 10g approximately Locate tubes correctly in tube holders Load tube holders in balanced configurations Distribute tubes in sample holders symmetrically in both directions Genevac Ltd will accept no responsibility fo
18. Benutzerhandbuch bevor sie sicherungen entfernen Danger Refer to manual before removing this cover Danger se reporter au manuel avant de retirer ce couvercle Achtung lessen Sie bitte das Benutzerhandbuch bevor sie die abdeckung entfernen Warning This equipment must be earthed Attention cet appareil doit tre branche a une prise de terre Warnung dieses Gerat muss geerdet sein FS6 T6 3A FS7 6 Supply 230V 50Hz The 2 mains supply fuse holders F6 and F7 are standard screw type located next to the mains power connector on the left hand side of the evaporator viewed from the front Fuse holders F1 F2 F3 F4 and F5 are quick release bayonet type and are located on the back panel left hand corner viewed from the front of the evaporator If a fuse blows more than once please contact Genevac Service Department To ensure uninterrupted service always ensure a spare fuse is available Section 3 Chapter 3 Page 2 10 1610 Issue 1 January 2005 EZ Bio Evaporating System Owners Manual Chapter 4 Lamp Assembly 10 1610 Issue 1 January 2005 Section 3 Genevac EZ Bio Evaporating System Owners Manual 1 Introduction This chapter provides guidance in the removal replacement and refitting of Lamp Assembly 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipme
19. CONDENSER DRAIN VALVE KNOB WILL RESULT IN THE EZ Bio DISPLAYING ERROR CLS Drn AND PREVENTING THE RUN FROM STARTING 9 Connect mains supply lead supplied to EZ Bio 10 Connect suitable drain hose and container not supplied DURING ITEM 11 THE EXHAUST HOSE MUST BE FITTED THIS HOSE MUST THEN BE DUCTED TO A SUITABLE EXTRACTION UNIT AREA OR A FUME HOOD 11 Connect the long exhaust hose supplied and duct into fume hood This is particularly important if or other acids are being used To maintain a solvent vapour free environment around your EZ Bio we suggest that you G do use free space within the hood for storage of vessels containing solvents acids Users should also ensure that tubing leading to and from the solvent condenser leads solvents and solvent vapours away from the machine in safe manner n particular the exhaust tube must be fitted to the outlet from the condenser and lead away to the laboratory fume extraction system This will ensure that any residual vapour which passes through the condenser and pump does not cause damage to your system 10 1610 Issue 1 January 2005 Section 1 Chapter 1 Page 1 Genevac EZ Bio Evaporating System User Manual Consumer Packaging 1 T Before signing for package or before opening case check that the tip n tell s are not activated If they are contact Genevac service UK Service 44 0 1473 243000 USA Service 1 845 267 22
20. Introduction Safety and Maintenance Notes Special tools and equipment Removal Refitting Testing Completion NOORWN AO0 Page ii 10 1610 Issue 1 January 2005 Genevac EZ Bio Evaporating System User Manual Chapter 7 Pump Head Components 1 Introduction Safety and Maintenance Notes opecial tools and equipment Removal Refitting Testing Completion NO Chapter 8 Condenser Drain Valve Knob Assembly 1 Introduction 2 Safety and Maintenance Notes 3 Special tools and equipment 4 Removal o Refitting 6 Testing 7 Completion SECTION 4 TECHNICAL SPECIFICATION Mechanical Data Vacuum system Temperature controls Condenser Data Solvent compatibility Dimensions Power Supplies Power Consumption Environment Operating Storage Emissions EC DECLARATION OF CONFORMITY SAFETY Warranty Statement USEFUL INFORMATION 10 1610 Issue 1 January 2005 Page iii Genevac EZ Bio Evaporating System User Manual 1 Introduction The product of detailed market research amongst biologists the EZ Bio is part of an integrated development programme based on our highly successful EZ 2 solvent evaporation platform The EZ Bio uses advanced and proven technology in evaporation science to solve the common problems of solvent removal in Life Science The new EZ Bio Personal Evaporator is all a rugged and modern centrifugal evaporator should be and more The EZ Bio is suitable for low to medium boiling point solv
21. PROVED SPARE PARTS Traces of solvents may be present that could be harmful to your health or lead to material damage There may be a risk to sample integrity required vacuum is not achieved 10 1610 Issue 1 January 2005 Section 3 Chapter 8 Page 1 Genevac EZ Bio Evaporating System User Manual Removal Do not attempt to replace O rings as high performance elastic has been used 1 4 m 4 1 Ensure that evaporator is switched OFF 4 2 Ensure that condenser has been fully drained and flushed 4 3 Fully unscrew drain valve anticlockwise and remove 5 Refitting 5 1 Unpack replacement Drain Valve Knob Assembly 5 2 Carefully insert into condenser 5 3 Carefully screw valve fully clockwise until resistance is felt 6 Testing 6 1 No formal testing is required 7 Completion 7 1 Return suspect Drain Valve Knob Assembly to Genevac Section 3 Chapter 8 Page 2 10 1610 Issue 1 January 2005 EZ Bio Evaporating System Owners Manual SECTION 4 TECHNICAL SPECIFICATION 10 1610 Issue 1 Januar y 2005 Genevac EZ Bio Evaporating System Owners Manual Mechanical Data Max RPM Max G Drive system Sample load including swings Max imbalance IR lamps number Vacuum system Pressure display Automatic Pressure control Bump protection System ultimate pressure Auto vacuum vent valve Temperature controls Temperature control range Tempe
22. TATIONS Evaporation of Acid Chlorides Evaporation of Di ethyl ether Evaporation of High Boiling Point Solvents Solvent Group Guide Genevac and the ATEX Diective Power Up Starting a Run Pre cool Stage Changing the Run Setting Run Time During a run Pausing a Run Stopping a Run Shutdown Out of Balance Defrosting Defrost Override Draining Lid Messages Cleaning SECTION 2 FAULT FINDING DIAGNOSTICS Safety Interlocks and Errors Audible Warnings Other Troubleshooting User Vacuum Test 10 1610 Issue 1 January 2005 Page Genevac EZ Bio Evaporating System User Manual SECTION3 MAINTENANCE Spares and Consumables Request Chapter1 Software Introduction Safety and Maintenance Notes Special tools and equipment Preparation Uploading Testing Errors Completion CONDOR hapter2 Drive Belt Introduction Safety and Maintenance Notes Special tools and equipment Removal Refitting Testing Completion XJONAGON O0 Chapter 3 Fuses 1 Introduction 2 Safety and Maintenance Notes 3 Special tools and equipment hapter 4 Lamp Assembly Introduction Safety and Maintenance Notes Special tools and equipment Removal Refitting Testing Replacement of individual components of lamp assembly Completion hapter 5 Lid Seal Introduction Safety and Maintenance Notes Special tools and equipment Removal Refitting Testing Completion NOoORWN AO0 hapter6 Pump
23. apter 5 Lid Seal Chapter 6 Pump Chapter 7 Pump Head Components Chapter 8 Condenser Drain Valve Knob Assembly With the introduction of the EZ Bio Genevac is now offering it s customers the ability to request spares and consumables online The EZ Bio is the first Genevac product to benefit from this new web based feature To request your spares and consumables online click on the link below or copy and paste it into your browser http www genevac com contact ezspares html 10 1610 Issue 1 January 2005 Section 3 EZ Bio Evaporating System Owners Manual Chapter 1 Software 10 1610 Issue 1 January 2005 Section 3 Genevac EZ Bio Evaporating System User Manual 1 Introduction This chapter provides guidance in the updating of Software Software Data Key 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipment Description Software Data Key Machine and Software requirements ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS N MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS OBSERVE PRECAUTIONS ELECTROSTATIC SENSITIVE DEVICES gt 10 1610 Issue 1 January 2005 Section 3 Chapter 1 Page 1 Genevac EZ Bio Evaporating System User Manual 4 4 1 4 2 4 3 9 1
24. ation with full sample protection throughout e High throughput design As well as being productive the EZ Bio is also highly versatile It s high capacity design accommodates wide selection of sample holders including 64 x 1 5ml centrifuge tubes 4 shallow well microplates 100 x 0 5ml centrifuge tubes round bottom flasks up to 500ml tubes up to 160mm long and HPLC vials 10 1610 Issue 1 January 2005 Page v Genevac EZ Bio Evaporating System User Manual 2 Safety and Maintenance Notes 2 1 Symbols The following safety symbols are used throughout this manual The definitions and scope of each symbol is as described below WARNING DAMAGE OR POTENTIAL SERIOUS INJURY Tienen nenes HAZARDS THAT CAN LEAD SERIOUS MATERIAL Caution This symbol provides information about hazards that can be harmful to your health or lead to material damage n Note This symbol provides information about technical requirements which if not followed can lead to malfunctions inefficiency and reduced productivity This symbol indicates that there may be a risk to sample integrity ARE READ TO ENSURE THAT THE IMPLICATIONS TO THE SAFETY OF PERSONNEL OPERATING THE SYSTEM AND FOR THE PROTECTION OF SAMPLE INTEGRITY ARE A BEFORE OPERATING THE SYSTEM IT IS IMPORTANT THAT THE FOLLOWING NOTES UNDERSTOOD Samples in the chamber are subjected to accelerations of up to 500G with a maximum load capacity of 1
25. c EZ Bio Evaporating System User Manual EC Declaration of Conformity We Genevac Limited Declare that this product EZ Bio Evaporating System Complies with the relevant Essential Health and Safety Requirements of the European Machinery Directive 89 392 EEC as amended by 91 368 EEC and 93 44 EEC The EMC Directive 89 336 EEC and the Low voltage Directive 73 23 EEC Conformity is demonstrated by compliance with the following specifications EN 60204 1 1998 Safety of machinery Electrical equipment of machines Pt 1 General Requirements EN 249 1992 Safety of machinery Safety distances to prevent danger zones being reached by upper limbs EN 1088 1996 Safety of machinery Interlocking devices associated with guards Principles of design and selection BS EN ISO 12100 pts 1 amp 2 2003 Safety of Machinery Basic concepts general principles for design BS EN 50082 1 1998 Electromagnetic compatibility Generic immunity standard BS EN 61010 2 020 1995 Safety requirements for electrical equipment for measurement control and laboratory use Particular requirements for laboratory centrifuges Safety A WARNING THIS SYSTEM MUST BE EARTHED THIS EVAPORATOR IS SAFETY CLASS 1 PRODUCT ACCORDING TO IEC CLASSIFICATION IT MUST NEVER BE USED WITH ANY INTERRUPTION TO THE SAFETY EARTH CONDUCTOR IT IS AN INSTALLATION CATEGORY Il PRODUCT AND IS INTENDED TO OPERATE FROM A NORMAL SINGLE PHASE SUPPLY
26. ective padding between evaporator and bench surface 4 5 4 6 4 7 4 8 4 9 4 10 4 12 With assistance move evaporator to suitable bench and carefully lower evaporator onto its back Remove drive belt access panel securing screws Remove drive belt access panel Visually check condition of belt ensure free from splits or tears If drive belt is intact and condition is acceptable check tension using special tool 04 3658 Rotate the pulleys so that the tightest part of the belt is at the measurement point This is done by feel only Mark this point on the belt using marker pen or chalk Place the supplied rubber band nominally 160mm long 1mm thick 6mm wide max over plain part of pulleys Ensure band is sitting flat and not on either the groove or the edge Slide the tensioning tool tool between the belt and the elastic band at a position along the belt immediately adjacent to the tapped holes Ensure min gap between the tool shaft and the base of the system 4 13 Hold the tool such that a steady load can be applied to the rubber covered end il During item 4 14 the yellow end of the tool body should meet the mark of 3 5kg Do not hold the body of the tool 4 14 Compress the tool so that the small spindle is just touching the elastic band 4 15 If required adjust tension as detailed in item 5 Section 3 Chapter 2 Page 2 10 1610 Issue 1 January 2005 Genevac EZ Bio Evaporating System User Man
27. ed when the rotor has stopped spinning and the pressure is at atmosphere 2 Therun will continue a reduced features mode This means that the EZ Bio has detected a failure in something that does not have any effect on Sample Integrity The physical condition of the evaporator Safety Critical An error code will be displayed when the rotor has stopped spinning and the pressure is at atmosphere INDICATION CAUSE OF ERROR RECTIFICATION AT START AAA VE Ensure lid is closed Lid not closed when run started Lid Drain left open Close drain Started with Lid or Drain open Press START button DURINGRUN Lid mid run Open and re close lid if required Loc mid run Open and re close lid if required dru bAL Err Failure to pull a Vacuum or Check lid seal UAC Vacuum fails mid run Check drain valve zZ Check condenser cap Check pump connections if disturbed MISCELLANEOUS 0 Rotary Run Selector Switch in Ensure Rotary Run Selector Switch is in 33 incorrect position correct position SEL incorrect position correct position drn 2 Turn drain knob anti clockwise approx 4 turns Lid 06 solenoid unlocking before lifting lid Almost all error messages can be cancelled by holding the Stop button down for 5 seconds 10 1610 Issue 1 January 2005 section 2 Page 1 Genevac EZ Bio Evaporating System User Manual Audible Warnings Critical Error User Warning Audible warning format S
28. ents in the range up to 120 C There are pre programmed methods for DNA purification and extraction oligosynthesis protein purification and removing aqueous mixtures There is even a special method for HPLC fractions such as Acetonitrile water mixtures Simple controls include temperature and time setting whilst a large backlit LCD display shows run status time temperature and pressure COOLING VENTS DEFROST BUTTON SIGHT GLASS CONTROL PANEL DRAIN TAP LID POWER SWITCH Fig 1 Page iv 10 1610 Issue 1 January 2005 Genevac EZ Bio Evaporating System User Manual The use of the optional high efficiency solvent resistant Teflon diaphragm pump which is integral to the evaporator saves you space and provides quiet efficient control of the vacuum Combined with a new visible glass condenser trap and easy access to solvent drain valve the EZ Bio is our most compact and reliable sample evaporator yet LAMP OFF BP lt 75 C OR MIXTURE BP 75 C 2 DNA PELLET BP lt 50 C TH Dri Pure START A SET RUNTIME PN v an LID Fig 2 EZ Bio Control Panel Key elements in the design present real advantages for molecular biologists protein and peptide chemists as well as general purpose solvent removal needs in life science laboratories e Ultra compact design to save precious bench space e Easy to use with intuitive controls and large LCD display e Fast evapor
29. full of volatile solvent run Condenser fuse failed Refer to maintenance section problem Vacuum stops pulling down at System waitng for This is correct and no action is required and pump life Boiling of solvent in condenser No problem This occurs normally Drain valve not fully Wind drain valve knob fully in closed Drain valve seal damaged If this affects the run then stop run and inspect replace seal Lid will not open despite OPn Lid displayed Lid seal stuck to glass Allow the solvent to evaporate before due to excessive solvent further use on the lid seal Section 2 Page 2 10 1610 Issue 1 January 2005 Genevac EZ Bio Evaporating System Owners Manual Other Troubleshooting Continued SYMPTOM CAUSE ACTION Lid is loose The lid is designed to This is correct an no action is required makes it feel loose Runs take longer than expected Check lamp is illuminated by looking through lid glass and replace if failed Vacuum leak Carry out user vacuum test appropriate set for the compound glass Clean IR sensor lens Caution refer to sensor lens maintenance section Incorrect sample holder Consult Genevac for advice for sample format Samples dry but not dry enough Consult Genevac for advice this Excessive quantity of solvent present Incorrect run selected Check that the run selected is most in catchpot exhaust condenser appropriate Or poor solvent recovery Condenser full Ensure c
30. hutdown Errors Series of 10 long beeps as run ends Repeats every 5 minutes until any user interaction Button pressed Run select switch moved Lid opened Run finished Three long beeps when system comes to rest Repeats every 5 minutes until any user interaction Button pressed Run select switch moved Lid opened Key Acknowledge Short beep Illegal key press Short double beep Other Troubleshooting SYMPTOM CAUSE ACTION Mains switch not illuminated Supply failure Check the system is switched on and supply is available Refer to maintenance section LCD not operating LCD not illuminated system in shutdown mode Press start button button flashing defrosting continue for a minimum of 40 mins Run does not appear to start Chamber pre cooling Pre cool is indicated on the display The run will start when the system is ready Error has occurred Check display for information messages and take appropriate action See previous table Vacuum fails to pull down below 900mbar at start of a run Lid not sealing Stop run and check for obstructions fit and condition of lid seal Replace if required Vacuum pulls down to 500mbar but DriPure The system is running correctly rate slows dramatically Vacuum pulls below 900mbar at start Drain valve seal or Carry out user vacuum test to verify of a run but takes longer than usual to condenser pot lid seal problem achieve a level of vacuum damaged glass assembly Condenser
31. ill be allowed to proceed The remaining defrost time is displayed on the screen Whenever the DEFROST button is illuminated DEFROST can be cancelled Once DEFROST has been completed you will be promted to drain the condenser Defrost Override When LCD displays dEF and defrost button is rapidly flashing simultaneously press and hold down the DEFROST button and the STOP button for 10 secs DEFROST MUST NEVER BE CANCELLED OR OVERRIDEN IF THERE IS ANY DOUBT OVER N WHETHER OR NOT FROZEN SOLVENTS REMAIN IN THE CONDENSER Genevac recomends that you always defrost and drain before umm Switching off Changing solvent types System acknowledges the override with a beep and prompts you to drain as normal This cannot be overridden 10 1610 Issue 1 January 2005 Section 1 Chapter 3 Page 7 Genevac EZ Bio Evaporating System User Manual Draining Access to the tap for the drain valve is provided on the right hand side of the machine towards the rear It is a multi turn valve turn counter clockwise to open The valve itself is made of glass PTFE attached directly to the bottom of the condenser and is at sufficient height to allow easy gravity draining into an appropriate receptacle The drain connection located on the right hand side of the machine is fitted with a male thread to allow connection of a drain tube via separate hose tail Supplied ei MA XTEMp When the drain has been opened the LCD will displa
32. ining the bracket 7 3 Remove the lamp by pulling firmly from the ceramic holder 7 4 Remove the glass and O ring 7 5 Fitnew ring on both sides of lamp ring During item 7 6 ensure that the Red Robax lens is correctly orientated 7 6 Fit new glass ensure glass is clean 7 7 Replace bracket with new lamp if required 7 8 Refit 4 off pozi drive screws with spring washers and tighten hard 7 9 Refit assembly as in item 5 above 7 10 Test assembly as in item 6 above Completion 8 1 Place defective lamp assembly into packaging for despatch back to Genevac 8 2 Return Genevac Section 3 Chapter 4 Page 4 10 1610 Issue 1 January 2005 Genevac EZ Bio Evaporating System Owners Manual Chapter 5 Lid Seal 10 1610 Issue 1 January 2005 Section 3 Genevac EZ Bio Evaporating System Owners Manual 1 Introduction This chapter provides guidance in the removal replacement and refitting of Lid Seal 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipment Description LID SEAL E BE ee ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS Traces of solvents may be present that could be harmful to your health or lead to material
33. lid Unlock button mid run Open and re close lid if required Err dru Motor or Belt failure mid run Stop run remove samples and check drive belt Err bAL Out Of Balance Trip Check samples balanced to within 2109 Vacuum fails mid run Refer to Section 2 of Owners Manual Err 33 or Rotary Run Selecter Switch in run SEL incorrect position position Condenser requires defrosting Press Defrost button OPn drn oystem needs draining 1 Connect suitable drain tube 2 Turn drain knob anti clockwise approx 4 turns OPn Lid Lid Open activated Open Lid Err 06 Tried to open lid before it is Reset and try again this time listen for solenoid unlocked unlocking before lifting lid Section 1 Chapter 2 Page 2 10 1610 Issue 1 January 2005 EZ Bio Evaporating System Owners Manual Chapter 3 DETAILED OPERATION 10 1610 Issue 1 January 2005 Section 1 Genevac EZ Bio Evaporating System Owners Manual SOLVENT LIMITATIONS Evaporation of Acid Chlorides Do not attempt to evaporate this class of solvent in your system Evaporation of Di ethyl ether Do not attempt to evaporate this solvent in your system Evaporation of High Boiling Point Solvents Genevac does not recommend the EZ Bio for use with solvents whose boiling points at atmospheric pressure are 170 C or above This includes DMSO DMI and NMP Solvent Group Guide SOLVENT ABBREVIATION BEST PROGRAM TO USE Acetic acid DCM methylene chloride 1
34. n 3 Chapter 1 Page 2 10 1610 Issue 1 January 2005 EZ Bio Evaporating System Owners Manual Chapter 2 Drive Belt 10 1610 Issue 1 January 2005 Section 3 Genevac EZ Bio Evaporating System Owners Manual 1 Introduction This chapter provides guidance in the removal and refitting of Drive Belt BELT TENSION DRIVE BELT BELT TENSIONER DRIVE MOTOR SECURING SCREWS 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipment Description DRIVE BELT BELT TENSIONING TOOL ALLEN KEY 6mm 5 ____________ pM AE ENSURE THAT EVAPORATOR IS ISOLATED FROM MAINS POWER SUPPLY ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS 10 1610 Issue 1 January 2005 Section 3 Chapter 2 Page 1 Genevac EZ Bio Evaporating System User Manual 4 4 1 4 2 4 3 4 4 Removal Remove ALL samples sample holders and swings from evaporator Drain condenser and ensure that condenser access cap Is secure and drain valve closed Ensure that the lid is closed and locked Failure to do so could result in damage to the system when placing evaporator on its back side Disconnect mains power supply i During item 4 5 place prot
35. nt Description LAMP ASSEMBLY STANDARD IO BEE Lamp assembly individual components NA LENS STANDARD 04 2948 1 LAMP AC9060 1 O RING p AAA ALLEN KEY 2 5mm 04 1125 ALLEN KEY 4mm AB9603 ENSURE THAT EVAPORATOR IS ISOLATED FROM MAINS POWER SUPPLY N ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS Traces of solvents may be present that could be harmful to your health or lead to material damage There may be a risk to sample integrity if lamp fails to operate or required vacuum is not achieved 10 1610 Issue 1 January 2005 Section 3 Chapter 4 Page 1 Genevac EZ Bio Evaporating System User Manual Removal 4 1 Open lid LAMP ASSEMBLY MAY BE HOT 4 2 Allow lamp to cool if system used recently 4 3 Disconnect mains power supply During item 4 4 note locations of screws as they are of different lengths 4 4 Using allen key remove screws qty 2 long screw to the front from lamp access panel located on the right hand side viewed from the front 4 5 Remove panel 4 6 Disconnect lamp power lead bulkhead connector by unscrewing collar suggest using left hand 4 7 Loosen and remove thumb screw THUMB SCREW CONNECTOR 4 8 Carefully withdraw lamp assembly Section 3 Chapter 4 Page 2 10 1610 Issue 1 Ja
36. nuary 2005 Genevac EZ Bio Evaporating System User Manual 5 Refitting 5 1 Unpack replacement lamp assembly and retain packaging LAMP CHAMBER SEAL BULKHEAD CONNECTOR COLLAR 5 2 Check that lamp chamber seal is located correctly 5 3 Locate lamp assembly bracket into guide 5 4 Offer up to chamber and ensure assembly locates securely 5 5 Fit and tighten thumb screw 5 6 Connect lamp power lead to bulkhead connector 5 7 Ensure collar is rotated clockwise 74 turn 5 8 Refit access panel and secure with screws qty 2 long screw to the front in locations noted at item 4 4 DO NOT USE SYSTEM WITHOUT PANEL BEING FITTED 5 9 Reconnect Mains power supply 6 Testing 6 1 Select H2O 6 2 Set temperature to 60 C 6 3 Press START button 6 4 Ensure that pressure drops to below 500 mbar and continues to drop i If pressure fails to drop check that lamp seal is correctly located on chamber wall and repeat items 6 1 to 6 4 inclusive 6 5 Carry out required run ensure that desired pressure is achieved 6 6 Check that lamp illuminates 10 1610 Issue 1 January 2005 Section 3 Chapter 4 Page 3 Genevac EZ Bio Evaporating System User Manual 7 Replacement of individual components of lamp assembly It is acceptable to replace the glass O rings amp or lamp individually by dismantling the lamp assembly 7 1 Remove the lamp assembly as in items 4 1 to 4 7 above 7 2 Unscrew 4 off pozi drive screws reta
37. ondenser drained after every run Vacuum leak Verify by user vacuum test and check causes above Condenser fuse failed Refer maintenance section Volatile solvent Some very volatile solvents will not be trapped in the internal condenser Body of system gets hot This is acceptable under normal operation use a Genevac external exhaust Excessive vibration High out of balance Ensure balanced to avoid vibration condenser Excessive noise Incorrectly fitted pump Remove and refit pump appropriate started have skipped the pre heat Cannot unlock and open lid lid LED Spin down timer still Wait until timer reaches zero then try not lit active again Lid button pressed too Wait for system to re enable lid open often in 5 minutes mechanism LED will re light i If action fails to resolve problem contact Genevac User Vacuum Test 1 Start defrost cycle this will take a minimum of 40 minutes 2 When any ice frost is thawed inside the condenser pot remove the cap and flush with volatile solvent eg acetone see Cleaning 3 Leave the cap off and the drain valve open during defrost 4 On completion of the minimum defrost time 40 minutes stop defrost refit cap and close valve Start H2O run 10 1610 Issue 1 January 2005 Section 2 Page 3 Genevac EZ Bio Evaporating System User Manual SECTION 3 MAINTENANCE Chapter 1 Software Chapter 2 Drive Belt Chapter 3 Fuses Chapter 4 Lamp Assembly Ch
38. r any loss or damage incurred by improperly or excessively loaded rotors Locate sample blocks correctly in sample swings Before starting or restarting a run check the following Ensure that both sample holders swing freely by swinging them by hand after loading The white PTFE washers located on each rotor pin are in place and in good condition All tube plates are correctly located Use only tubes that are able to withstand significant loads Medium or thick wall Bora Silica glass tubes with a wall thickness of no less than 1 2mm are recommended Do not use worn or scratched tubes or vials Do not load tubes or vials into sample holders other than those types that have been approved by Genevac Ltd Do not use sample holders that have not been supplied with this system without consulting Genevac Service 3 2 Lid operation The lid has an electrical lock and is opened and closed manually after pressing the LID unlock button There is a safety device fitted that prevents the lid from being unlocked and opened whilst the rotor is rotating or until the chamber pressure has equalised 3 3 Limitations of use Accuracy of IR Sensor is dependant upon condition of Sample Holders and Swings To ensure correct operation Sample Holders and Swings are to be kept clean and free from contamination and corrosion Your evaporating system is unsuitable for use under the following circumstances e With strong mineral acids such as HCI and HBr a
39. rature control accuracy Temperature sensing Temperature display range Chamber lid temp range Condenser Data Condenser temperature Vacuum pots number Useable Capacity vacuum pot Materials condenser pots Drain valve Solvent compatibility Boiling points Dimensions Width Depth Height Weight Power Supplies 120V 10 60Hz 15A 230V 10 50Hz 13A 10 1610 Issue 1 January 2005 2000 500 G Direct 1 5 kg 40g 0 1200 mbar 10 mbar atm Yes 10 mbar Yes Ambient to 80 C 2 5 C infra red pyrometer 99 to 99 C Ambient 40 C 1 1 litre Borosilicate glass Glass PTFE Isolast Up to 130 C 540 mm 650 mm 687 mm 84 5kg USA UK Europe Section 4 Page 1 Genevac EZ Bio Evaporating System User Manual Power Consumption Peak 230V 50 2 1610VA 120V 60 2 1800VA Note The systems may momentarily take current in excess of the figures quoted above Genevac therefore recommends the use of appropriately rated type D circuit breakers on the main supply to the EZ Bio Environment The following figures apply Operating Ambient Temperature 15 C to 30 C Relative Humidity 10 60 Altitude Sea Level to 1 600m Storage Ambient Temperature 10 C to 60 C Relative Humidity 10 80 Altitude Sea Level to 12 000m Emissions Noise levels do not exceed 55dBA at one metre from the evaporator Section 4 Page 2 10 1610 Issue 1 January 2005 Geneva
40. s HH MM The Dri Pure LED indicator will be lit during any Dri Pure enabled run During the Dri Pure part of a run the word Ramping will appear below Pressure on the LCD display Pausing a Run Press the Pause button COUNTDOWN TIMER System will vent and stop the rotor coundown timer will count from 96 down to 0 seconds The lid can then be opened and the samples examined The run can be re started within the next 5 minutes by closing the lid and pressing Start If Dri Pure had finished before the pause button was pressed then Dri Pure will not be re applied During the 5 minutes wait for a restart the Time Remaining indicator will be counting down in MM SS from 05 00 mins Pause mode can be cancelled by pressing Stop while paused or by leaving the machine in pause mode for longer than 5 minutes You can restart the run by pressing the PAUSE button again 10 1610 Issue 1 January 2005 Section 1 Chapter 3 Page 5 Genevac EZ Bio Evaporating System User Manual Stopping a Run This can be manual or automatic Manual Press the Stop button while in the run The system will vent and then the rotor will be brought to a halt Automatic Without any user input the run will be automatically terminated when the Time Remaing reaches 00 00 The system will then vent and the rotor will be brought to a halt so ending the run Once the run has ended the system beeps 3 times and flashes the Lid button to a
41. t The condenser and pump will remain turned off until the Start key is pressed at which point both are powered up Starting a Run The LCD display then becomes illuminated showing pressure temperature and an time remaining of 00 00 See the paragraphs on Defrosting and Draining if the system prompts you DEFROST BUTTON flashing i Note that the lid will remain locked when there is no power to the system Section 1 Chapter 3 Page 2 10 1610 Issue 1 January 2005 Genevac EZ Bio Evaporating System User Manual To open the lid press LID button please wait the 3 seconds for the lid to release then open the lid The lid remains unlocked for 10 seconds once the LCD indicates OPn Place your samples into the EZ Bio Close the lid Select desired Solvent Type from rotary selector Select the required rotor temperature on the control knob Press START button When the start button is pressed the system will check that the lid is closed and locked and the drain valve is closed then begin the run See the paragraph on Lid Messages to view displays In either case a second press of the start button is then required to spin up the rotor and begin the run The display will prompt you with PSH Strt while waiting lt MAXTEMp If the run is not started within the 30 minutes of reaching the minimum chamber temperature required then the run will be cancelled 10 1610 Issue 1 January 2005 Section
42. t all concentrations EVAPORATING DIETHYL ETHER AND SIMILAR LOW AUTO IGNITION SOLVENTS e For use as a pressure vessel 10 1610 Issue 1 January 2005 Page vii Genevac EZ 2 amp 2 2 Evaporating System Owners Manual SECTION 1 GENERAL Chapter 1 UNPACKING AND SETTING UP 2 0 2 e cece e eect Chapter 2 a APP PP ee Chapter 3 DETAILED OPERATION casio eee ee 10 1610 Issue 1 January 2005 Section 1 EZ Bio Evaporating System Owners Manual Chapter 1 UNPACKING AND SETTING UP 10 1610 Issue 1 January 2005 Section 1 Chapter 1 Genevac EZ Bio Evaporating System Owners Manual Flight Case 1 Undo 4 quick release fasteners 2 Carefully remove lid Refer to photographs on Pages 2 to 4 inclusive of this Chapter for aditional guidance 3 Position base and EZ Bio near to operating bench location 4 With assistance 3 people total lift EZ Bio from base and position where required on bench 5 Ensure that there is 50mm clearance from edge of bench and other equipment 6 Refit lid and store flight case T Check contents of accessories container 8 Remove Condenser Drain Valve transportation blank retain and fit Drain Valve by carefully inserting into condenser and screwing home clockwise until resistance is felt DO NOT USE THE CONDENSER DRAIN VALVE KNOB TO MOVE OR LIFT THE EZ Bio N FAILURE TO REMOVE BLANK AND FIT
43. ttract attention to the end of run Shutdown Shutdown is when the pump and backlight is shutdown the condenser may or may not be shutdown This can be manual or automatic Manual To shutdown simply press the Stop button when in a run The pump will be purged for 30 minutes before finally switching off i The condenser will not be stopped unless the system has been defrosted and drained Automatic The system will also shutdown automatically if left unattended for 15 minutes at the end of a run This allows the evaporator to be loaded at the end of the day safe in the knowledge that it will turn itself off when done The display will clear to the software version and the LCD backlight will turn off The pump will turn off after the purge time as defined above has elapsed The compressor will not switch off in standby mode if automatically turned off Out of Balance Should the evaporator be loaded incorrectly or go out of balance during a run the system will end the run automatically venting and bringing the rotor to rest safely The LCD will show Err DAL Section 1 Chapter 3 Page 6 10 1610 Issue 1 January 2005 Genevac EZ Bio Evaporating System User Manual Defrosting If you switch ON and START the system without previously having DEFROSTED the system you will be prompted to DEFROST The DEFROST button will flash rapidly to inform you that it is to be pressed and the system defrosted before a run w
44. ual 4 16 If belt tension cannot be achieved replace belt as detailed in items 4 18 to 4 21 inclusive 4 17 f belt is broken remove belt and check condition of drive pulleys 4 18 Loosen the 4 drive motor securing screws maximum 3 turns 4 19 Loosen the lock nut on the adjuster screw 4 20 Rotate belt tension adjuster fully anticlockwise to loosen belt 4 21 Remove belt 5 Refitting 5 1 Ensure that the 4 drive motor securing screws are loosened 5 2 Ensure that belt tensioner is fully unscrewed anticlockwise 5 3 Position replacement belt over drive pulleys i During item 5 4 check belt tension with special tool 5 4 Screw in belt tension adjuster clockwise to tighten belt until correct tension is obtained 5 5 Tighten the 4 drive motor securing screws 5 6 Rotate belt one complete revolution 5 7 Recheck belt tension and adjust accordingly 5 8 Tighten lock nut on adjuster screw against chassis 5 9 Recheck belt tension and adjust accordingly 6 Testing 6 1 Ensure that rotor rotates freely by hand 7 Completion 7 1 Replace belt access panel 7 2 Refit securing screws 7 3 With assistance carefully stand the system back upright on it s feet Once upright it is acceptable to open the lid again 7 4 Reconnect mains power lead 10 1610 Issue 1 January 2005 Section 3 Chapter 2 Page 3 EZ Bio Evaporating System Owners Manual Chapter 3 Fuses 10 1610 Issue 1 January 2005 Section 3
45. uitable work surface 4 2 Remove punp from tray by removing attachment screws 4 3 Note and mark position of PTFE tubes 4 4 Disconnect the 2 PTFE tubes from the connectors on the pump head 4 5 Using Allen Key remove the 4 retaining screws from the pump head Fig 2 4 6 Remove pressure plate 4 7 Carefully remove the connecting head 4 8 Carefully remove valves qty 2 and ring seals qty 2 4 9 Carefully remove pump head Section 3 Chapter 7 Page 2 10 1610 Issue 1 January 2005 Genevac EZ Bio Evaporating System User Manual 4 10 Using Pulley Spanner carefully locate in Strain Washer and rotate anticlockwise Fig 4 4 11 Remove Strain Washer Diaphragm and Pressure Disc assembly PRESSURE PLATE CONNECTING HEAD O RING PUMP PRESSURE DIAPHRAGM STRAIN SEALS amp HEAD DISC WASHER VALVES 4 12 Separate the three items 10 1610 Issue 1 January 2005 Section 3 Chapter 7 Page 3 Genevac EZ Bio Evaporating System User Manual 5 Refitting 5 1 Remove replacement components from service kit packaging 5 2 Ensure that all items are correct Fig 5 LL E Tue C TES Do not use any assembly oils or fluids when reassembling the pump Clean distilled water may be used to locate seals and valves during assembly 5 3 Assemble Strain Washer Diaghragm and Pressure Disc Fig 5 5 4 Carefully locate onto pump connecting rod Fig 6 5 5 Using Pulley Spanner carefully
46. ump tray assembly FASTENERS HA POWER CONNECTOR Fig 1 4 Head Pump 4 4 Disconnect pump power connector i During item 4 5 the PTFE hoses are left connected to the pump 4 5 Carefully note position of the 2 PTFE hoses and disconnect from evaporator 5 Refitting 5 1 Unpack replacement pump and retain packaging 5 2 Remove blanking caps from the two hoses and retain 5 3 Position pump assembly and connect PTFE hoses to positions noted at item 4 5 5 4 Connect pump power connector i During item 5 5 ensure that the PTFE hoses and cables are not trapped or distorted 5 5 Carefully slide pump assembly into back of evaporator 5 6 Secure with the fasteners qty 4 Section 3 Chapter 6 Page 2 10 1610 Issue 1 January 2005 Genevac EZ Bio Evaporating System User Manual 6 6 1 6 2 6 3 6 4 Testing Reconnect mains power supply Power up evaporator Select H20 Press START button IF PUMP LINES ARE INCORRECTLY FITTED THE PUMP COULD PRESSURISE THE SYSTEM N CAUSING DAMAGE TURN SYSTEM OFF IF PRESSURE INCREASE OCCURS DURING ITEM 6 5 6 6 6 7 7 1 7 2 6 5 Ensure that pressure drops to below 500 mbar and continues to drop If pressure fails to drop repeat items 4 1 to 4 3 inclusive and check condition of PTFE hoses and connections repeat items 6 1 to 6 5 inclusive Carry out required run ensure that desired pressure is achieved Completion Fit hose blanking
47. y drn count down from 10 seconds El MAXTEMp o If the drain valve is open when you try to start a run the LCD will display CLS drn to prompt you to close the drain El MAXTEMp o Section 1 Chapter 3 Page 8 10 1610 Issue 1 January 2005 Genevac EZ Bio Evaporating System User Manual Lid Messages If the lid is not closed when the start button is pressed you will be told to close it with the message CLS Lid Cleaning Condenser Remove the cap from the top of the condenser Pour cleaning fluid into the condenser Clean using a bottlebrush Ensure suitable container is fitted to drain hose Open drain tap and allow cleaning fluid to drain into container Dispose of contaminated cleaning fluid in accordance with local regulations 10 1610 Issue 1 January 2005 Section 1 Chapter 3 Page 9 EZ Bio Evaporating System Owners Manual SECTION 2 FAULT FINDING DIAGNOSTICS Safety Interlocks and Errors Audible Warnings User Vacuum test Trouble shooting 10 1610 Issue 1 January 2005 Section 2 Genevac EZ Bio Evaporating System Owners Manual Safety Interlocks and Errors If an error occurs during run then one of 2 things will happen 1 The run will be terminated This means that the EZ Bio has detected a failure in something that may have an effect on Sample Integrity The physical condition of the evaporator Safety Critical An error code will be display

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