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Auxiliary states devices

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1. 0 to 100 This control is disabled for manually controlled states The Rapid is rather different from the other two devices as it is capable of producing a train Type of stimulator Magsim Rapid of pulses at high rates Rather than the simple gh use with Mann ER case mounted manual controls of the other two Com port for communications TOM 1 gt units the Rapid has a separate control system T lgnere coliintedock swich A that has to be disconnected to gain access to the Use Rapid single pulse mode serial line control port limiting the usefulness Q manual control of manual control There are two controls u z2 governing overall behaviour e power 30 2 pulses at 10 Hz This disables checks on the coil interlock switch on the handle of the Magstim coil so Settings for state 2 that the unit will fire without a button being T Manual Power 30 held down Magstim do not recommend Pulses 2 Freq Ha f0 disabling these checks as they are a safety Test feature OK Cancel Help This turns on single pulse mode which allows higher power levels up to 110 In single pulse mode only a single pulse is produced per trigger and the pulse train parameters are ignored The pulse rate can be up to 100 Hz normally depending upon the power level used see the user manual but if you select power levels above 100 the maximum allowed pulse rate is forced to 0 5 Hz With single pulse mode turned off a train of up to
2. the 1401 to trigger the 1401 sweep Consult your Magstim documentation for more information Notes on Magstim use A straightforward arrangement to use a Magstim with Signal would be as follows 1 Connect the serial port of your computer to the Magstim serial line input using the appropriate serial line cable Connect the trigger BNC plug to the 1401 digital output port 0 BNC socket found on the front of all modern types of 1401 If you are using a 1401 plus the digital output pulse is available from the 25 way digital output socket on the front of the 1401 2 In the outputs page of the sampling configuration make sure that digital output bit zero is enabled for use Using the pulses configuration dialog set the initial level of digital output bit zero to 0 and place a pulse which will be high going in the outputs at the time when you want the Magstim to fire This output pulse should be at least 10 microseconds long 1 millisecond works well 3 The Magstim support uses Signal multiple states sampling which should be set up in dynamic outputs mode You can use any number of extra states each extra state providing separate Magstim settings Each set of pulse outputs should include digital output pulses to trigger the Magstim along with any other outputs required The Magstim support will work correctly with manual control of the states or with any style of automatic states sequencing including protocols 22 6 CED 3304 auxiliary de
3. the basic CED 3304 parameters and enables checking of CED 3304 behaviour during sampling and the recording of the stimulation settings 22 9 Signal for Windows version 4 CED 3304 Connections and cabling 3304 serial cable The CED 3304 uses a USB A B cable or a 9 pin serial line cable both are supplied with the unit for control Use one cable or the other If you plug in both the USB connection will take precedence See the Operation chapter of the CED 3304 Owners Handbook for more information about the control cables The front panel Trigger input should be connected to the 1401 digital outputs or other TTL pulse source using a standard BNC cable The front panel Data and Clock inputs are not used with Signal and should be left unconnected CED 3304 PC serial 9 Way D Type 9 Way D Type socket socket CABLE SCREEN Notes on 3304 use A straightforward arrangement to use a CED 3304 with Signal assuming we use digital output bit 0 to control the current pulse timing would be as follows 1 Connect your computer to the CED 3304 using the either a serial or use a USB connection Connect the CED 3304 front panel Trigger BNC plug to the 1401 digital output 0 BNC socket found on the front of all modern types of 1401 If you are using a 1401plus the digital output pulse is available from the 25 way digital output socket on the front of the 1401 2 In the Outputs page of the sampling configuration make sure that digital ou
4. using this configuration two separate serial lines are used to control the Magstims and two separate trigger pulses are required as for a BiStim in independent trigger mode When the dual 200 option is selected a separate selector for the second serial line port is shown but as for the 200 there are no controls governing overall behaviour The settings available for the individual states are Magstim configuration xi Dual Magstim 200s J Allow use with Magstim not present com 1 7 com 2 x Type of stimulator Com port for communications Com port for second unit manual control power 30 power 2 30 dual trigger 2 power 30 power 2 dual tric power 30 power 2 30 dual trigger er Power f30 Power 2 f30 Copy to all Copy above OK Cancel This control sets the main master stimulator aca eral power output as a percentage of the maximum available you can set any value from 0 to 100 This control is disabled for manually controlled states Set this checkbox for this state to be controlled J Manual by the manual controls and not by Signal clear it to give Signal control Settings for state 2 This control sets the second stimulator power output as a percentage of the maximum available you can set any value from O to 100 This control is disabled for manually controlled states There are two connector arrangements provided by Magstim for serial line cont
5. 1000 pulses can be generated at the specified frequency from one trigger and power levels above 100 are not available Again the pulse rate can be up to 100 Hz depending upon the power level used The Rapid settings available for the individual states are Set this checkbox for this state to be controlled by the manual controls and not by Signal clear it to give Signal control As you have to disconnect the manual controls in order to connect the serial line used by Signal I fear that this option will not be particularly useful This sets the stimulator power output as a percentage of the maximum available you can set any value from 0 to 100 This control is disabled for manually controlled states This item is only available if single pulse mode is not in use it sets the number of pulses you can set any value from 1 to 1000 This control is disabled for manually controlled states or if single pulse mode is selected This item is only available single pulse mode is not in use it sets the pulse frequency in Hertz you can set any value from 0 to 100 This control is disabled for manually controlled states or if single pulse mode is selected 22 4 Dual 2007 devices Manual Power Power 2 Magstim Connections and cabling Cabling for 26 way combined serial and trigger connector Auxiliary states devices Two Magstim 200 s can be controlled by Signal to achieve much the same effect as using a BiStim when
6. BiStim will be set with a resolution of 1 millisecond With this checkbox set the maximum interval is reduced to 99 9 milliseconds but the interval timing resolution is improved to 0 1 milliseconds This control is disabled if independent trigger mode is selected The BiStim settings available for the individual states are Set this checkbox for this state to be controlled by the manual controls and not by Signal clear it to give Signal control This sets the main master stimulator power output as a percentage of the maximum available you can set any value from O to 100 This control is disabled for manually controlled states 22 3 Signal for Windows version 4 Interval Power B Rapid device Ignore coil interlock switch Use Rapid single pulse mode Manual Power Pulses Freq This sets the interval between the two pulses you can use any value from 0 to 999 milliseconds or 0 to 99 9 milliseconds for Hi res timing Setting a value of zero for a state will switch the BiStim into simultaneous pulse mode setting a value greater than zero switches it out of simultaneous mode While switching back and forth between modes during an experiment is possible it may increase the inter sweep delay This control is disabled if independent triggers are in use or for manually controlled states This sets the second slave stimulator power output as a percentage of the maximum available you can set any value from
7. Magstim auxiliary device support Magstim Safety notice introduction Auxiliary states devices When sampling using multiple frame states Signal can control external devices such as stimulators in addition to switching the 1401 outputs This is achieved by using auxiliary states devices external equipment that is set up in different ways according to the sampling state Signal auxiliary states device support provides a mechanism for controlling arbitrary equipment and setting it up in different ways according to the sampling state in use In addition to controlling equipment settings by state Signal is also able to provide device specific configuration dialogs to check the equipment health and readiness while sampling to save the stimulation parameters used in the new data file and to save and load auxiliary states setup information to and from sampling configurations Script language functions SampleAuxStateParam and SampleAuxStateValue are also provided to give access to the auxiliary device settings Signal currently supports two types of auxiliary states device the Magstim range of transcranial magnetic stimulators and the CED 3304 programmable constant current stimulator You can select which of these devices you wish to use or none during the Signal installation process if you wish to change the type of auxiliary states device in use the simplest way to do this is to re install Signal If you need auxiliary states device sup
8. cause the Magstim to ignore the coil interlock switch and to enable single pulse mode for the Rapid This documentation only describes the controls available from within Signal for a complete understanding of the effect these have upon the behaviour of the stimulator you should consult the Magstim user manuals In addition to using a serial line to set the stimulation parameters standard operation with Signal requires that the stimulation be triggered using one or more TTL pulses generated by the 1401 digital outputs This allows the pulse timing to be precisely controlled relative to the Signal sampling A Rapid can be configured to either generate a train of pulses in response to a single trigger or to generate one pulse per trigger allowing complete control of the patterns of stimulation Manual triggering of the 22 1 Signal for Windows version 4 Magstim configuration stimulator is also possible as is triggering from other external hardware in which case you would use the Magstim trigger to trigger the sampling sweep as well The Magstim support monitors the stimulator health both waiting until the Magstim is ready for use before allowing a sampling sweep to proceed and terminating sampling if a hardware problem develops To aid data analysis Signal saves the Magstim stimulation parameters used in the data section variables of the sampled file The parameters saved are the power level the pulse interval BiStim only t
9. cted state may be edited 22 2 200 device Manual Power Copy Test BiStim device BiStim in independent trigger mode Use Hi res timing mode Manual Power Auxiliary states devices This is the simplest type of Magstim and does not require any controls for overall behaviour The settings available for the individual states are Set this checkbox for this state to be controlled by the manual controls and not by Signal clear it to give Signal control This sets the stimulator power output as a percentage of the maximum available you can set any value from 0 to 100 This control is disabled for manually controlled states The buttons labelled Copy to all and Copy above can be used to set up many states quickly Copy to all copies the currently selected state to all other states while Copy above copies the current state s settings to all higher state numbers These buttons are available for all types of Magstim The button labelled Test tests if communications with the Magstim can be established It checks that you have the correct COM port that the serial line is connected and that the Magstim is communicating correctly with Signal These buttons are available for all types of Magstim The BiStim is more complex than the 2007 in essence it is two 2007 units with one slaved to the other When a BiStim device is selected two controls governing overall behaviour are shown This sets h
10. ded that the CED 3304 is not qualified for human use It is your responsibility to ensure that Signal s control of a CED 3304 is set up in an appropriate and safe fashion for the intended use This documentation only describes the CED 3304 controls available from within Signal for a complete understanding of the effect these have upon the behaviour of the stimulator you should consult the CED 3304 Owners Handbook 22 7 Signal for Windows version 4 CED 3304 support configuration Hardware selection Com port for communications The CED 3304 produces current pulses in one of four current ranges 10 and 100 microamps and 1 and 10 milliamps The current range is selected by a manual switch on the CED 3304 You specify a current range when you define the stimulus settings in Signal Signal will not let you sample if the wrong range has been set on the CED 3304 The timing of the 3304 current pulses is controlled by a TTL signal connected to the front panel Trigger input Signal can control the timing of the current pulses with one of the 1401 digital outputs in Dynamic outputs mode or external equipment can control the pulsing in Static outputs mode For safety reasons the CED 3304 will switch off a pulse after a preset limit is reached see the 3304 documentation for details of how to control this limit Signal monitors the stimulator health waiting until it is ready for use before allowing a sampling sweep to proceed and termi
11. he secondary power level BiStim and dual 200 the pulse frequency Rapid only and the pulse count Rapid only These values are placed in the first user frame variables in the sampled data file The saved values can be retrieved using the script language later versions of Signal may make use of them directly To configure a Magstim in Signal open the sampling configuration dialog and make sure that Multiple states is enabled in the General tab Select the States tab and make sure that you are in Dynamic outputs or Static outputs mode If Magstim support is installed there will be a button labelled Magstim If this button is not present reinstall Signal and make sure that you select Magstim auxiliary states device support If the button is present but disabled you are in External Digital states mode change the mode to enable the button Click the button to open the Magstim configuration dialog It is meaningful to use the Magstim support without using multiple states but this is not directly supported because the states system is so closely linked to the Magstim support If you want to make use of Magstim support without using multiple states so that the pulse parameters are recorded and Magstim health checks are made but with complete manual control you should turn on multiple states set only one extra state set state zero to use manual control and only make use of state zero while sampling This will allow you access to the Magsti
12. lected state At the bottom are buttons to copy settings get help test the CED 3304 and accept the dialog settings Hardware selection Com port for communications Current range selection Active high trigger current 0 microamps current 10 microamps current 20 mictroamps current 30 microamps current 40 microamps current 50 microamps Set this to Use CED 3304 to use the stimulator or to Do not use if you do not want to make use of the 3304 support software in this sampling configuration Settings for state 2 Current microamps Signal connects to the CED 3304 either via a f2o Copy to all Copy above Test serial communication port or through a USB cme Hep port which is setup as a virtual COM port This field sets the COM port that the CED 3304 is connected to If you use the USB connection follow the instructions at the start of the Operation chapter of the CED 3304 Owners Handbook to obtain a suitable device driver for your operating system 22 8 Current range selection Active high trigger Seitings for state Copy Test Simple use Auxiliary states devices This field sets the maximum current that you can enter for each state You can select from 10 and 100 microamps and 1 and 10 milliamps This control matches the current range rotary switch on the front of the CED 3304 but does not override its setting the actual range switch setting must match the setti
13. m configuration dialog to set the basic Magstim parameters At the top of the configuration dialog there is a selector used to select the Magstim type You Tipe A Aiae Magstim 200 F should set this to the type of stimulator you i i I Allow use with Magstim not present will be using or to Do not use if you do not Eom port for communications om a want to make use of the Magstim support in this sampling configuration If you set the device type incorrectly this will not always be detected though errors during sampling will manual control ower 30 D i Z 2 power 30 almost certainly result Below the device zae a paer ae selection is a selector for the COM port used to State 5 power 30 control the stimulator you can select any port from COMI to COM9 As many PCs only Settings for state 1 have one COM port you may have to use a T Manual Power fao USB serial port expander to provide a spare COM port we have found that most of these Co py to all Copy above Test USB serial ports work satisfactorily E Hep Below the basic stimulator configuration there are controls that vary according to the type of stimulator selected These are in three sections first there are controls which govern the overall behaviour for all states next is a display of settings for all states which is used to select a state for editing by clicking on the information for that state and finally an area where the settings for the sele
14. nating sampling if a hardware problem develops To aid data analysis Signal saves the stimulation current in the data section variables of the sampled file This value in uA is placed in the first user frame variable in the sampled data file The saved values can be retrieved using the script language later versions of Signal may make use of them directly To configure the CED 3304 in Signal open the sampling configuration dialog and make sure that Multiple states is enabled in the General tab Open the States tab and make sure that you are in Dynamic outputs or Static outputs mode If CED 3304 support is present there will be a button labelled CED 3304 If this button is not present reinstall Signal and make sure that you select CED 3304 stimulator support If the button is present but disabled you are in External Digital states mode Change the mode to enable the button Click the button to open the CED 3304 configuration dialog From the configuration dialog you can enable use of the CED 3304 set the serial line port used to communicate with the hardware and other overall parameters define the current settings for each state in use set the active level for the Trigger input and test for successful communications with the CED 3304 use cep 3308 gt At the top of the dialog are settings that apply to all states In the centre is a list of the currents set for each state Below this is a field to edit the current for the se
15. ng in this dialog or an error will be generated when sampling is begun Check the box for an active high trigger current is generated while the CED 3304 Trigger input is at a high TTL level Clear the box for active low operation current generated when the Trigger input is at a low TTL level When you are driving the trigger input from the 1401 digital outputs it is usual to set an active high trigger This field displays and lets you edit the desired current for the state selected in the list of states You can enter any value in microamps from zero to the maximum available for example in the 10 uA range you could enter 0 or 8 234 or 10 The buttons labelled Copy to All and Copy above can be used to set up many states quickly Copy to All copies the currently selected state to all other states while Copy Above copies the current state s settings to all higher state numbers This button tests if communications with the CED 3304 can be established It checks that you have the correct COM port that the serial line is connected and that the CED 3304 is operating correctly The Help Cancel and OK buttons have their usual meanings If you want to use the CED 3304 from Signal very simply with a single current setting you must still enable multiple states Set one extra state so states are enabled and set the desired current for state zero and then sample using state zero only This gives you access to the configuration dialog to set
16. ow Signal will expect the BiStim to be configured rather than how it will be set up Magstim configuration Eg Magstim BiStim bai Type of stimulator by Signal Normally a BiStim generates two output pulses one at the time of the trigger and the second at a preset interval after the trigger Using the controls on the front of the BiStim master unit you can set the device into I Allow use with Magstim not present com 1 7 J BiStim in independent trigger mode I Use Hi res timing mode Com port for communications manual control power 30 power b 30 after 50 ms independent trigger mode referred to as IBT mode in the Magstim documentation When this mode is in use two separate triggers are used presumably signals from two 1401 digital outputs one trigger firing the main pulse and the other firing the second Power B pulse Because a BiStim behaves rather differently when in IBT mode it is important that you set this checkbox correctly to match the BiStim setup you will be using otherwise errors will be generated when you try to sample power 30 power b 30 after power 30 power b 30 after 50 ms Settings for state 3 T Manual Power f30 Interval ms 50 Power B f30 Copy to all Copy above Test Cancel Help When this checkbox is clear the interval between the two pulses can be set to any value between 0 and 999 milliseconds the actual timing in the
17. port for other equipment please contact CED The Magstim range of transcranial magnetic stimulators are widely used to provide non invasive neuronal and muscular stimulation Signal provides support all three types of Magstim the 200 BiStim and Rapid as the software uses a serial line for control of the stimulator only the modern Magstims that provide serial line control these have a 7 suffix to the model name can be used In addition Signal can also control a pair of Magstim 200 units using two serial lines Transcranial magnetic stimulators are dangerous devices capable of causing serious harm and should only be used by qualified medical practitioners Before using a Magstim device you should read the user s manual produced by Magstim paying particular attention to the warnings and precautions section It is your responsibility to ensure that Signal s control of a Magstim is set up in an appropriate and safe fashion for the intended use and to verify that it is operating correctly CED is not responsible in any way for problems caused by use of this software For Magstim devices the auxiliary states system sets the power output and where appropriate inter pulse timing remote control of the hardware can be disabled for specific states to allow the user to control the stimulation manually Options are provided to cause Signal to assume independent triggering mode and to enable high resolution interval timing for the BiStim and to
18. rol and external triggering of the unit Older 200 and BiStim units have an arrangement with a 9 way serial line and separate 15 way trigger connector while newer 200 and BiStim units and all Rapids have a high density 26 way combined serial line and trigger connector In both cases two separate connections need to be made serial line control from a PC and a TTL trigger signal from the 1401 The standard high level trigger input suggested by Magstim works well with Signal and the 1401 and is used in the diagrams shown below consult the Magstim documentation if you require a different arrangement In the case of a Rapid stimulator the 26 way connector is also used by the integrated control system and this will need to be disconnected before the control cable can be connected Magstim PC serial 26 Way D Type 9 Way D Type high density plug socket CABLE SCREEN Trigger BNC plug to 1401 digital out 22 5 Signal for Windows version 4 Cables for separate 9 pin Magstim PC serial serial and 15 way trigger 9 av D Type 9 Way D Type plug r socket connectors Serial cable CABLE SCREEN Magstim Trigger 15 Way D Type BNC plug plug to 1401 digital out Trigger cable If you are not using the external trigger input to trigger the Magstim from the 1401 you will need a different cabling arrangement for example to take the Magstim low going trigger out signal on pin 8 of the 26 way connector and use that as an input to
19. selected by Signal beforehand If manual control is selected the Magstim will disarm itself spontaneously only after 60 seconds have passed without a stimulus trigger so it is your responsibility to make sure the Magstim is disarmed if manual control is used and Signal encounters a significant problem The Rapid stimulator does not appear to disarm itself after 60 seconds in this manner and must be disarmed manually if Signal fails during sampling Because Signal needs to communicate frequently with a Magstim to stop it disarming scripts that operate while Signal is sampling need to be correctly designed If a script carries out a lengthy operation without yielding or using a toolbar or dialog to allow control to pass back to the operating system this may interfere with Magstim communications and cause the unit to disarm The CED 3304 current stimulator can be used to generate controlled constant current stimulations of up to 10 milliamps and is fully controlled by Signal allowing you to set a different level of current for each state Signal controls the CED 3304 programmable constant current stimulator using a serial line or USB port to interact with the hardware The CED 3304 current stimulator can generate outputs of up to 90 volts which lies within the potentially lethal range Before using the device you must read the CED 3304 Owners Handbook paying particular attention to the warnings and precautions section In particular you are remin
20. tput bit zero is enabled for use Using the pulses configuration dialog set the initial level of digital output bit zero to 0 and place a pulse which will be high going in the outputs at the time when you want the CED 3304 to fire This output pulse should be as long as the required stimulation 3 The CED 3304 support is designed to work with Signal multiple states sampling which should be set up in Dynamic outputs mode Set one extra state for each output current setting you require and each state should generate digital output pulses to trigger the CED 3304 plus any other outputs required The CED 3304 support will work with manual control of the states or with any style of automatic states sequencing including protocols When Signal begins sampling with the CED 3304 support enabled it checks for a correctly functioning device If a CED 3304 is found Signal will check the range switch setting initialize the device and enable the trigger While sampling is in progress Signal sets the output current using the state data before each sweep At the start and end of each sweep the CED 3304 health is checked to make sure it is OK When sampling finishes the CED 3304 trigger is disabled Because of the extra overhead in setting up current levels for each sweep and checking that the device is operating correctly you may find that the maximum sweep rate with the CED 3304 is reduced 22 10
21. vice support CED 3304 Safety notice Introduction Auxiliary states devices When Signal begins sampling with the Magstim support enabled it checks for a correctly functioning Magstim device as part of the process of initialising for sampling If a Magstim is found then Signal will carry out the initial configuration of the Magstim and arm the device While sampling is in progress Signal will set up the Magstim using the current state data before each sweep it will then delay the start of each sweep until the Magstim reports that it is armed and ready At the end of each sweep the Magstim health is checked to make sure it is OK Note that the checks on Magstim readiness can impose a significant extra inter sweep delay though steps have been taken to minimise this When sampling finishes normally the Magstim is disarmed and remote control disabled If the Magstim coil temperature rises too high Signal will stop sampling Once the coil temperature has dropped sufficiently you can press More on the sampling control panel to resume sampling again While sampling is in progress Signal continuously maintains communications to prevent the Magstim from disabling remote control and disarming itself If Signal ceases to communicate with the Magstim because it has encountered a significant problem crashes the Magstim will disarm itself automatically within 1 second but this safety feature only applies if manual control has not been

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