ELEVATED PLUS MAZE
Contents
1. 0 5 10 etc Size of Array C was set to 2 with the DIM command which means that Array C was defined for 3 data points Array O was defined for 3 data points and array R was defined for 8 data points Each array has been defined in the comments section at the beginning of the mpc file That information was used to add the following labels to each array The lines were expanded to make room for the labels Array C Raw Data Array C is used for closed exploration closed entrance and time These values represent the data obtained from the radial arms with the attached walls 0 2 000 2 000 70 834 Array C with Labels C 0 C 1 C 2 Closed Plosed Entrance Time in Closed Row Marker Exploration Runways 0 2 2 70 834 Array O Raw Data Array O is used for open exploration open entrance and time These values represent the data obtained from the open sided radial arms 0 2 000 2 000 103 302 Array O with Labels O 0 O 1 O 2 Time in n Open Exploration Open Entrance ms Row Marker Runways 0 2 2 103 302 Array R Raw Data Array R is used to for total runway data Elements 5 6 and 7 are internal variables that help with the control of the program Their values are not important Ox 0 000 1 000 1 000 1 000 1 000 5 4 000 4 000 8 000 Array R with Labels R 0 R 1 R 2 R 3 R 4 Full Entrances to Full Entrance to Full Entrances to Full Entrance to Runway 1 Runway 3 H2. ERA RU T eta eed bess 15 Breakdown of Sample File Simple Variables ssssssse mm 16 Breakdown of Sample File Arrays ssssssseee meme 16 MED ASSOCIATES INC ELEVATED PLUS MAZE CHAPTER 1 Introduction The purpose of this manual is to give an explanation of the MED State Notation Procedures that comprise the SOF 700RA 3 Elevated Plus Maze Procedures The files in this package can be found on the disk provided by MED Associates Inc These procedures are intended to be run in MED Associates MED PC IV software The latest version of MED PC IV gives researchers the ability to use pre programmed procedures such as these to make hardware control and data collection easy These pre programmed procedures can also be modified to meet the evolving demands of a research protocol Again it is the intent of this manual to explain exactly what these procedures implement and provide guidance into how to interpret what the program code achieves in order to let the user determine how to modify them to match their research protocol demands The manual provides some examples of editing and modifying the procedure s programming code The manual also defines the elements in the raw data file produced by these procedures In addition to this manual refer to the MED PC IV User s Manual for the installation of the MED Associates interface drivers the MED PC IV Software and the Delphi Compiler Also refer to the User s Manual
3. be recorded by the program the subject must break and then release both I R photobeams located at the entrance to each runway in succession Once an entrance is recorded the program assumes that the subject remains on the runway until returning to the junction area and the time spent in that runway is tabulated Figure 1 2 Entrance Diagram 5 EE 11 ERR 1 a er a Explorations AND An exploration is the event that occurs when the subject moves partially into one runway of the maze and then retreats back into the junction area In order for an exploration to be recorded the subject must break one or both I R photobeams located at the entrance of a runway and then retreat thereby releasing the outermost I R photobeam first If an exploration is recorded then the program assumes that the animal has returned to the junction Figure 1 3 Exploration Diagram One Beam Broken e e a LR mmo m m 1 11 1 2200 E Figure 1 4 Exploration Diagram Two Beams Broken ML HL M M NH ES 2 i ii E BN ET Tr Runway Time For the Elevated Plus Maze 1 mpc program the amount of time the subject spends in each of the four runways is tallied once the I R photobeam most proximal to the junction is interrupted In the data file runway time is displayed in seconds Junction Time Junction time is calculated by the program and represents the total amount of time s
4. settings have been made turn the recorder off again by using the main menu or tool bar Save the macro with a distinctive name The example in Figure 3 13 was named EPM_SessionTime_30 mac since the Session Time was changed to 30 Figure 3 13 EPM_SessionTime_30 mac p D EPM SessionTime 30 MAC Notepad File Edit Format View Help LOAD BOX 1 SUBJ EXPT 0 GROUP 0 PROGRAM ELEVATED PLUS MAZE 1 A SET session Time min VALUE 30 MAINBOX 1 BOXES VW Once this macro is built use the macro editor to make simple changes such as replacing reward or correct lever values Review the Help file on screen or the MED PC IV User s Manual for more information on macros and the features offered A START command or message box followed by a START command could be added to the macro it was left off here so changes could be verified before starting the procedure MED ASSOCIATES INC ELEVATED PLUS MAZE Modifying the MedState Notation Code Permanent changes to the Elevated Plus Maze procedures can be made to the MedState Notation code To make the same change to the Session Time as shown above do the following Open Trans IV and select File Open to place Elevated Plus Maze 1 mpc into the text editor Scroll down to approximately line 97 note the line counter in the lower right hand corner of the editor to reveal the code shown in Figure 3 14 Figure 3 14 Elevated Plus Maze 1 mpc Line 97 ES TRANS IV C MED PC IV MPC El
5. D PC IV Load Wizard MED PC IV is designed to help the researcher run an experiment by guiding selection choices through its Experiment Loading Wizard This section will describe how to initiate the Elevated Plus Maze 1 mpc application however the following steps that will also apply to all other mpc procedures Open MED PC IV and the MED PC Experiment Loading Wizard s Welcome screen shown in Figure 3 2 will appear Figure 3 2 The MED PC IV Loading Wizard Welcome Screen To avoid this load wizard deselect the checkbox labeled Run this experiment automatically when starting MED PC Close this screen by clicking the Close button Closing this screen immediately reveals the MED PC Run Time Screen shown in Figure 3 9 If the choice to continue with the Loading Wizard is made then click the Next button MED ASSOCIATES INC ELEVATED PLUS MAZE The Box Selection screen will appear next as shown in Figure 3 3 From this screen the researcher chooses which boxes will be used in the experiment Select the boxes that will run the experiment by clicking in the radio button next to the box number The figure shows that the Hardware Configuration included only 1 box which was selected Click Next to continue Figure 3 3 The Box Selection Screen The Select a Procedure screen appears next as seen in Figure 3 4 This is where the application to be run is selected The screen displays a list of all the currently compiled proced
6. ELEVATED PLUS MAZE MED STATE NOTATION PROCEDURES SOF 700RA 3 Manual DOC 023 Rev 1 2 Copyright 2008 All Rights Reserved Med Associates Inc P O Box 319 St Albans Vermont 05478 www med associates com MED ASSOCIATES INC ELEVATED PLUS MAZE TABLE OF CONTENTS Leu T0 aii lg 1 l ATTOdUCEION mec 1 Overview of the Procedures ene eene nene nnn 2 Dependent Variables 2 ip p e EP D ESREELER E REESE HERE EE eb CERE in eds 3 Independent User Defined Variables 4 Chapter 2 oaoeseisiinnissaas5235838312212823835212138838583 8898088182 58 88 8888 088 828 8084 808 82 888A 0A 8A RR 5 Getting Start amp d u ie eicere eet ree rice PLUR oes eer ree hate Drbempo ra edad ries eae 5 Software Installatlori us esent tomes vk A am t eraut E T 5 Backing Up the Software edt PE tabes btt adiutor El xa bv iP De 5 Chapter Diarra sens san sans ena manne cessera 02 88A ER SR NERA EAR I0 8488 0 R0 MIRA RR 6 Beginning amp Running an Experiment 6 Translating The MED PC IV mpc File 6 Setting the Temporal Resolution 6 Using the MED PC IV Load Wizard 7 Viewing Changing Variable Values sss mene 12 prse ET 13 Modifying the MedState Notation Code 14 Chapter 4 15 Understanding the Data Files 15 Sample Data File ico rte b px kx IR Re Gives
7. are satisfied with the products and services we provide and look forward to meeting your future experimental needs as your research program evolves Thank you for purchasing the Elevated Plus Maze Data Collection Application Overview of the Procedures Elevated Plus Maze 1 mpc The Elevated Plus Maze 1 mpc procedure begins by placing the subject in the junction of the open and closed runways Two infrared I R photobeams are positioned at the entrance of each of the four runways of the maze and are used to track the subject as it explores each runway of the maze Runway time is counted when the animal starts exploring the runway Elevated Plus Maze 2 mpc The Elevated Plus Maze 2 mpc program is very similar to Elevated Plus Maze 1 mpc The only difference between these two programs is that Elevated Plus Maze 2 requires the subject to clear both I R photobeams before Runway Time is counted Therefore the Elevated Plus Maze 2 mpc program tabulates a much more conservative measure of Runway Time Both programs require that the MED PC IV resolution to be set to 2 ms or less see the Setting the Temporal Resolution section of this manual Figure 1 1 Overview of the Plus Maze MAZE SUPPORT RUNWAYS IR PAIRS ENV 256I ATH R UNDERNEATH RUNWAY REMOVABLE LEGS Dependent Variables Entrances When the subject moves completely into a runway of the maze it is recorded as an entrance For an entrance to
8. ce the value in the lower right hand box or use the up down arrows to increment by 1 Click the Issue button for the change to take effect Click Named Variables to produce the display in Figure 3 12 Change variables here as needed Figure 3 12 Displaying Named Variables from Box 1 Displaying Named Variables from Box 1 Start Delay sec Named Vars Session Time min 15 000 Vars Refresh Issue Close Help Add Display Data from Box Additional Boxes to Update Select All Deselect All I 12 ME NCCQOHCIATES INC FI EVATE p C MA a MED ASSOCIATES INC ELEVATED PLUS MAZE Macros The simplest way to initially create a macro is to record keyboard functions while performing the steps manually Once the commands are in the macro it is easy to create a number of macros with the macro editor The following example illustrates the process of loading Box 1 and changing the Session Time to 30 minutes To begin open MED PC IV and going directly to the run time screen Close the load wizard if present Before loading or opening the procedure click Macro on the main menu and select Turn On Macro Recorder or click the 8th tool bar item with the cassette tape icon on top A note on the bottom of the display indicates that the recorder is running Open Elevated Plus Maze 1 mpc by clicking Files Open Session Change the variables using any of the methods described above When all
9. evated Plus Maze 1 mpc ES File Edit Search Options Translation Window Help MT PR AAA Ch Ch h h ch ch B R B KK CK A ACA A TS 3 5 1 Si 0 001 SET A 5 B gt 2 Change B 15 to B 30 and save the changes with the same or a new file name such as EPM_SessionTime_30 mpc Remember if creating a new mpc file name and are using a macro to load boxes the file name in the macro also must be changed Translate and compile the new or changed file as described previously and run MED PC IV Use the Change Variables screen to view confirm the new values CHAPTER 4 Understanding the Data Files Unless otherwise specified data will be saved to C MED PC IV DATA Data can be saved manually by selecting FILE SAVE DATA MANUALLY or FILE SAVE DATA FLUSH The file name that is used to save the data in depends on the option that was chosen in the Hardware Configuration Utility and may also be dependent on the Subject Experiment and Group name provided in the MED PC IV load wizard Within each data file the headings are created for each Subject Experiment Group Box etc see below Data files may be opened with note pad word pad or any word processor or spreadsheet however be sure they are always saved unformatted in case a data extraction utility such as MED PC to Excel might ever be used Data file formats are explained in detail in the MED PC IV User s Manual Sample Data File Select Annotated on the file
10. for instructions on developing a Hardware Configuration Data file structure file saving format and other related options are also determined by the Hardware Configuration Running the Hardware Configuration software utility that accompanies MED PC IV sets the Hardware Configuration Its purpose is to assign the inputs and outputs on the interface cards in the interface cabinet for each task controlled by MED PC IV The particular type of interface card that is supplied in the interface cabinet may vary please refer to the User s Manual provided for instructions on how to configure the type of card that is in the cabinet A valid Hardware Configuration must exist in order for MED PC IV to interface correctly with the MED Associates Inc hardware This means that one should take the time to create a valid Hardware Configuration before attempting to run the procedures included in this package Should there be any problems the staff at MED Associates Inc is available to answer any questions that may arise Please e mail us at support med associates com with a detailed description of the problem or desired goals so that concise and detailed information may be provided The Elevated Plus Maze procedures are designed to be as easy to use as possible MED Associates Inc understands that researchers do not have the time to devote to programming and hardware design and for that reason we have undertaken that burden for you We sincerely hope that you
11. h loading the box or Previous to correct errors MED ASSOCIATES INC ELEVATED PLUS MAZE The Alter Session Parameters Screen shown in Figure 3 7 is the next screen to appear and is an important screen for the researcher The Alter Session Parameters screen allows the researcher to alter the parameters by which a procedure executes The Send Start Command Screen appears next The options available on the screen vary depending upon how many boxes are described in the Hardware Configuration Figure 3 7 Alter Session Parameters Screen In this example only 1 box is described in the Hardware Configuration so Figure 3 8 will appear next If more than 1 box is in the Hardware Configuration then Figure 3 9 will appear Figure 3 8 Send Start Command Screen for Single Box Configuration s 10e MED ASSOCIATES INC ELEVATED PLUS MAZE Figure 3 9 Send Start Command Screen for Multiple Box Configuration In both cases Figure 3 8 and Figure 3 9 the screens are where the researcher decides to either load more boxes send a start signal to boxes that are already loaded or enter the MED PC IV run time environment without sending a start signal by selecting am finished with the wizard This option results in the screen shown in Figure 3 10 Figure 3 10 The MED PC IV Run Time Screen MED PC IV Be x File Configure View Macros Help amp ie e alale amp epi 3 el Box Subject Experiment Grou
12. ng Trans IV before they can be executed in this application Be sure that a copy of the file being translated is present in the directory C MED PC IV MPC Open Trans IV icon and select Translation Translate and Compile Select the program s to use for the experiment and click Make Click OK to start the translator and it will automatically parse the MedState Notation and then open to a DOS screen to compile the Pascal code Depending on the speed of the computer each of these steps may not be seen If any problems are encountered during this process refer to the on screen help menu or the MED PC Version IV User s Manual or contact MED Associates Inc for assistance Figure 3 1 Trans IV Control Panel for Translating and Compiling MedState Notation Code i Specify Files to Translate Elevated Plus Maze 1 mpc Elevated Plus Maze 2 mpc Build No Trans Exclude wv OK X Cancel Help Setting the Temporal Resolution When running this application the temporal resolution setting in MED PC IV should be changed from 10ms to 2ms or less Failing to do so may result in errors when running this program Open the Hardware Configuration Utility by clicking Start Programs Med Associates MED PC IV Hardware Configuration Utility Then click Define Temporal Resolution Set the resolution to 2 then click OK and close the Hardware Configuration Utility MED ASSOCIATES INC ELEVATED PLUS MAZE Using the ME
13. on Marker TE closed Runway 2 open closed Runway 4 open 0 0 1 1 1 1 R 5 R 6 R 7 Active Arm A Beam B Beam Row Marker 5 4 4 8
14. options page during hardware installation to produce a raw data file similar to the following Data files are located in C MED PC IV Data unless an alternate path was defined during hardware installation They may be opened with note pad word pad or any word processor however make sure they are always saved unformatted in the occasion a data extraction utility such as MPC2XL is used The header information should be self explanatory Data file formats are explained in detail in the MED PC IV User s Manual File C MED PC IV DATA 2004 04 06 15h40m Subject 0 Start Date 04 06 08 End Date 04 06 08 Subject 0 Experiment 0 Group O0 Box 1 Start Time 15 40 24 End Time 15 43 34 MSN Elevated Plus Maze 1 A 5 000 B 15 000 J 6 576 S 180 000 Ci 0 2 000 2 000 70 122 O 0 2 000 2 000 103 302 R 0 0 000 1 000 1 000 1 000 1 000 CE 4 000 4 000 8 000 Breakdown of Sample File Simple Variables The following simple variables are shown immediately following the header information A 5 000 Start Delay in seconds B 15 000 Session Time in minutes J 6 576 Junction Time in seconds S 180 000 Elapsed Start Delay and or Session Time in seconds Breakdown of Sample File Arrays The arrays are presented in rows of 5 elements each The first row begins with element 0 and ends with element 4 the second row begins with element 5 and ends with element 9 etc Each row begins with an element marker followed by a colon
15. p Load Program 1 Subject 1 Experiment 1 Group 1 10 56 Elevated Plus Maze 1 Start Delay 5 00 SessionTime 1500 S 10 SHOWS 11 15 SHOWS 18 20 SHOWS 21 25 SHOWS 26 30 4 gt 12 17 2008 105823 AM ie lis MED ASSOCIATES INC ELEVATED PLUS MAZE Viewing Changing Variable Values Before a start command has been issued any variable may be changed on the MED PC IV run time screen Simply highlight the value to change and then enter the new value Once a session is in progress change variables by selecting Configure Change Variables or click the 4th tool bar item AX In the lower left hand corner of the Change Variables window find the Display Data from Box display and choose the chamber s to modify By clicking additional boxes in the Additional Boxes to Update section changes made to a single box are automatically loaded to all of the selected boxes Figure 3 11 Changing Variables Screen Displaying Variables from Box 1 A B D E Named Vars 5 000 15 000 C n 0 000 0 000 3 _Nemed Vas F G H k Jd Vars 0 000 0 000 0 000 0 000 0 000 zi K L M N 0 000 0 000 0 000 0 000 M Heres P Q S IT 0 000 0 000 manas 0 000 0 000 Issue U v W Y ci 0 000 0 000 0 000 0 000 0 000 BUS Z 0 000 Help Display Data from Box Additional Boxes to Update A from Box 1 5 000 x To change a value simply highlight and repla
16. pent in the junction area of the maze Independent User Defined Variables Session Time The default Session Time is 15 minutes but this is a user defined independent variable and the value for this variable is set in minutes Start Delay Start Delay is a function that gives the researcher time to start the program and then place the animal into the junction of the plus maze The session timer and data collection begin as soon as the start delay expires The default Start Delay is 5 seconds and this variable is set in seconds CHAPTER 2 Getting Started Software Installation Please refer to the MED PC IV User s Manual for a complete guide to installing the MED PC IV software building a valid Hardware configuration with the Hardware Configuration utility and opening and compiling a MSN procedure in the Trans IV utility To install the Elevated Plus Maze Procedures insert the CD into the CD ROM drive and click Install the Elevated Plus Maze Software The Elevated Plus Maze procedures are copied into the C MED PC IVAMPC folder Backing Up the Software Med Associates strongly encourages creating backup copies of the programs in case of disk failure Having copies of the original programs may be useful in the future should modifications be made to the existing programs CHAPTER 3 Beginning amp Running an Experiment Translating The MED PC IV mpc File Programs written in MedState Notation must be translated usi
17. ures Select the desired procedure and then click Next Figure 3 4 The Select a Procedure Screen ELEVATED PLUS MAZE 1 ELEVATED PLUS MAZE 2 MED ASSOCIATES INC ELEVATED PLUS MAZE The Enter Experiment Data Screen should display next as shown in Figure 3 5 The purpose of this screen is to allow annotations to be added to the data file that is produced by MED PC IV These annotations will help identify the Subject Experiment and Experiment Group upon which data was collected Comments can be added here as well and the data file can be given a customized file name to help identify it from other data files Enter the information desired and click Next Figure 3 5 Enter Experiment Data Screen s Subject 1 Subject r E Experiment 1 Experiment PAM G roup Group 1 Comments Elevated Plus Maze Experiment Optional Custom Filename levated Plus Maze Subject Groupi c amp De ES E e The next screen to appear is the Review Choices screen as seen in Figure 3 6 This is a method of confirming that the information received from the Box Procedure Selected is correct If it is not correct select Previous and edit the data If it is correct select Next Figure 3 6 Review Choices Screen Review the data below Boc 1 Procedure ELEVATED PLUS MAZE 1 Subject Subject 1 Experiment Experiment 1 Group Group 1 Comment Elevated Plus Maze Experiment Filename Elevated Plus Maze Subjectl Groupl Click Next to finis
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