seal-pup manual - Jena Bioscience
Contents
1. 7 Move the sealing table to the right opening the door and remove the sealed plate NOTE Take care as the foil may still be hot please allow to stand to cool before processing piercing peeling or storing 8 Apart from normal visual inspection it is not recommended for users to service this machine cleaning should be done using a mild detergent and damp cloth only Please do not attempt to touch hot internal surfaces E Troubleshooting Symptom No lights or temperature display No reaction when START pushed Plate wells melted or damaged Plate is sealed too well Inconsistent sealing across plate Plate sticks and travels up with heater head Possible cause Power not on Power cord not plugged in Blown fuse Sealing table not in correct position under heater head Parameters incorrectly set see D Plate not on adaptor correctly Temperature too low see DI Seal time too short see D2 Temperature too high seeD Seal time too long see D2 Plate warped try increasing seal temperature and or time Call Service Centre Foil upside down see D4 Temperature too high see D1 Seal time too long see D2 Heater head dirty see D8 F Hints In our tests best results have been found with the following parameters MF 100 Easy peel 100 pack 150 170 C 2 4 seconds MF 200 Durable 100 pack 160 180 C 2 5 seconds MF 300 Paper backed 100 pack 125 140 C l 4 seco2. Do not modify the system without our prior written approval Do not use the system for any other purpose than as described in the introduction Do not exceed 9 9 seconds or 200 C on the time temperature controllers The system weighs 68 Ibs or 3I kgs do not attempt to lift on your own Installation Unpack the sealer carefully and place on a level stable surface that can withstand its weight Do not block ventilation openings Remove all other parts from the box See H The sealing table is taped to the guide rails Remove the tape and screw in the handle Fix the guide bracket to the right hand side front with u shaped mounting bracket down using the Allen screws provided check that the sealing table moves freely from side to side Remove protective plastic film Plug in the power cord and switch to ON D Operation The user can adjust both temperature and sealing time SET THE TEMPERATURE by pressing the up down arrows to the desired temperature SV Press the SEL button to store in memory PV is the heating plates actual temperature a RS And S 2 SET THE SEALING TIME by pressing the buttons relating to the digit you wish to change on the face of the timer Values increment only going beyond 9 leads to a 0 When the desired value is shown press reset on this display to store the time in memory NOTE Please take care here that the display is showing seconds and that only one decimal p
3. of microplate to installation table in operation sealing position At Rear 14 Name rating plate Shows serial number etc 15 Concentric plug Country specific S180 type 15 A minimum
4. MiniSealer User Manual AXYGEN S C E N T I F Ff amp AXYGEN SCIENTIFIC INC 33170 CENTRAL AVENUE UNION CITY CA 94587 USA tel 510 494 8900 fax 510 494 0700 e mail info axygen com web www axygen com distributed by Jena Bioscience Table of contents A Introduction B Safety C Installation D Operation E Troubleshooting F Hints G Specifications H Technical Data page 3 page 3 page 3 page 4 page 5 page 6 page 6 page 7 A Introduction The MiniSealer is a manual heat sealer used for sealing any kind of microplate to prevent evaporation contamination or freezer burn It thermally bonds a number of different foils to microtitre cell culture PCR or even deep well plates The digital temperature and timer control allows user selection of parameters for different foils and plates Because of the inbuilt Pressure Sensing Press PSP the system is able to exert the same force 150kg in each cycle transferring heat evenly to the foil whether using shallow PCR plates or deep well plates a consistent seal every time Safety YOU MUST READ THIS N OA Ww A C Do not attempt to operate this system until you have read this manual thoroughly When any service or maintenance is being done ensure that the power supply is off and power cord removed Please be aware that the HEATER head stays HOT for up to one hour after the power supply is disconnected
5. lace is showing If this is not the case increment the buttons until MODE DOWN and RANGE are showing on the display Press the right hand button and cycle through 0 s 0 00 min s 0 00 h min 0 hr 0 000 s and 0 00 s until reaching 0 0s Press MODE DOWN will flash MODE KP OFF will flash and MODE You can now increment the time value by pressing the correct column button and press reset on this display to store that value in memory 3 Wait for 10 minutes after initial switch on to allow the temperature to become stable at the set point 4 Place plate on Sealing Table and position pee the required foil normally shiny side up on the microplate on the correct adaptor on the sealing table MALLIIN ai 5 Using the handle slide the sealing table all the way to the left to position it correctly under the heater head You will notice that the safety guard door closes automatically 6 Press the large green START button The heater head travels down to contact and applies heat and pressure to the foil You will see the time display decrement to show seal time left When that reaches zero the heater head will lift back to it s original position NOTE The user can interrupt the sealing cycle at any time by pushing the large yellow reset button on the main panel This is intended as a safety device that lifts the heating head back to its start position should there be a problem in the seal cycle identified by the user
6. nds Please note that ideal conditions vary dramatically depending on plate type G Specifications US Other Dimensions whd inches mm 10 allow 14 x I7 x 18 25 allow 36 x 43 x 46 Weight Ibs kg 68 3 Power 120 VAC 60Hz 230 VAC 50Hz Temperature Room to 200 C in degree increments Time 0 1 to 10 seconds in 0 1 increments Plate sizes 128 x 86 mm base up to 50 mm high Indoor use only Up to 2000 metres above sea level Only operate between 5 and 40 C Only operate with less than 80 RH H Technical data Drawing as before with these definitions E Timer E Start switch 4l Reset switch Shows both Push to Push to countdown operate cancel in and set emergency values ED Temperature controller fil fl Shows both actual and set values E Adaptor PCR and micro plate available for different formats 6 Power switch On off illuminated 13 Plexiglas safety guard Prevents hands from being inserted into the operational area Ze Safety gate Auto opening closing with movement of 12 Heater head sealing table The hot surface that lowers and makes contact with me kei 11 Sealing 10 Sealing 9 Sealing E3 Sealing table plate guide plate guide plate handle Slides in and out bracket bolt bracket Used to of right hand side Used in Used to support move sealing to allow transport initial and align sealing table
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