User`s Manual - Eagle Lake Property Owner`s Inc.
Contents
1. User s Manual Appendix B Internal Datalogging Bl Shipping Checklist Instruments with internal data logging purchased will also receive in their shipment Interface cable Turner Designs Spreadsheet nterface Software 2 disks Both of these items are necessary for retrieving the stored data in the Aquafluor B2 Hardware Requirements PC with Windows 95 or later MS Excel 5 0 or later Atleast available serial port B3 Installation 1 2 3 Exit all Windows programs Insert Disk and run the setup program The setup wizard will install the necessary files You w ill be prompted for Disk 2 when necessary When the setup is complete an icon named TD will be found in the Programs menu Restart your computer B4 Connecting 1 Using the provided cable connect the 9 pin adapter of the cable into the available serial port of vour computer Aquafluor User s Manual 0 Aquatluor User s Manual Data can also be transferred directly to the computer after Plug the opposite end of the cable into the base of the Aguatiuor Open MS Excel 5 0 or later Open the TD2 spreadsheet interface software Click on the box to the right of the COM port icon to select the appropriate COM port This is usually COM por 2 Click on Start The program will open an Excel spreadsheet for data transfer The boxes left of the COM port and MS Excel should both be green Follow the2. 5 Damage from seawater and other moderately corrosive materials that are not promptly removed from the instrument are not covered 6 Damage caused by modification of the instrument bv the customer is not covered Aquafluor User s Manual AEN 16 5 2 Warranty Service of Warranty Service To obtain service during the warranty period the owner 5 3 Out of shall take the following steps iy Proceed exactly as for Warranty Service above If our 1 Write or call the Turner Designs service department and service department 455151 a by phone or correspon Es ill be glad to at no charge describe as precisely as possible the nature of the dence we will be glad to at no charg problem i Repair service will be billed on a basis of time and materi als A complete statement of time spent and materials used will be supplied Shipment to Turner Designs should be prepaid Your bill will include return shipment freight charges 2 Carry out minor adjustments or tests as suggested by the Service Department 3 If proper performance is not obtained ship the instru ment prepaid to Turner Designs with a statement of e i r Shipment shipping charges The instrument will be repaired and Address fo returned free of charge along with a check to cover Turner Designs tral rar 845 W Maude Ave shipping charges for all customers in the contiguous United States Sunnyvale CA SUE For custom
3. Aquafluor Handheld Fluorometer and Turbidimeter User s Manual Dated 3 08 01 Version 1 1 P N 998 0851 B45 W Maude Avenue Sunnyvale CA 94085 408 749 0694 FAX 408 749 0098 www lumerdesigns com Toll free 1 877 316 5049 TURNER DESIGNS Table of Contents l Introduction 1 1 Description xis 1 2 Inspection and Setup saison 1 3 General Information snd bit 2 Quick View Diagrams sesssesssssssesssesssssosssesssssess 3 Instrument Parameters 31 Firmware Flowchart 8 32 Instrument Power Up MO 33 Fluorescence or Turbidity Chanidl ac 10 34 Calibration Standard Value 10 35 Calibration sias 36 Sample REN 37 Internal Data Logging IDL 3 7 Activate Data Lessing O gt 372 Download Data 12 3 7 3 Erase Data assem AZ 38 Diagnostic Information 12 4 General Considerations for Analysis 41 Handling Samples 42 Linear Range and Quenching 13 43 Temperature Considerations 15 44 Positioning Samples 15 45 Data Quality I5 5 Warranty 5 Terms A TEE TNE SATE 52 Warranty d ice 53 Outof Warranty Bervi EN Aquafluor
4. signal over a 5 second interval The result will be displayed at the top and center of the Home screen The top left corner will display WAIT for 5 seconds Once WAIT disappears another sample reading can be performed Internal Data Logging IDL This is an optional feature If this feature has been pur chased your Aguafluor can log up to 1000 data points The DATA screens control logging downloading and erasing the data For further information see Appendix B Aquafluor User s Manual 11 3 7 1 Activate Data Logging l Press the DATA button 2 times 2 Press ENT to toggle between logging and stop statuses 3 Press lt ESC gt when finished to return to the Home screen 3 7 2 Download Data Connect the Aquafluor to the serial port of your computer 2 Open the Turner Designs Interface Software See Appendix B for computer requirements and installation 3 Press the DATA button 3 times 4 Press lt ENT gt 5 times to start the data download 5 Press lt ESC gt when finished to return to the Home screen 3 7 3 Erase Data l Press the DATA button 4 times 2 Press ENT 5 times to erase all logged data 3 Press lt ESC gt when finished to return to the Home screen 38 Diagnostic Information l Press DIAG to access the diagnostic screens 2 The first screen shows the number of data points available for internal data logging 3 Press lt ENT gt to toggle to the FS Full Scale valu
5. User s Manual amp sam gt T M 1 2 1 Introduction Appendices LI Description A Instrument Specifications Al General Specifications sss 19 A2 Optical and Application Specifications 19 The Aguafluor is dual channel mini fluorometer designed for quick easy and accurate fluorescence and turbidity measurements When properly calibrated with a standard of known concentration the B Internal Data Logging Aquafluor displays the actual concentration of the Bl Shipping Checklist iia compound B2 Hardware Requirements 2D B3 Installation di 2 Inspection and Setup DE Connecting silage d binae BS Real Time Data Transfer 2 1 2 1 Inspection i IDL Troubleshooting 22 Upon receiving your instrument please inspect everything carefully and make sure all accessories are present All shipments include C In Vivo Chlorophyll BEREBERE 33 The Aquafluor The User s Manual 4 AAA batteries 4 Polystyrene cuvettes Storage Pouch 1 2 2 Setup Before the Aguafluor be used the supplied batteries must be installed the backside of the instrument loosen the screw and remove the battery panel see Section 2 for diagram 2 Install the 4 AAA batteries into the appropriate spaces 3 Replace the battery panel and tighten t
6. and temperature The light history of an algal population will affect fluorescence of livi ing cells Cells in a darker environ ment will fluoresce more per unit chlorophyll than cells in a well lit zone of the water column One w ay of reducing the effects of light is to dark adapt your sample before analyzing it Temperature effects are discussed in section i Ld O E 2 e 3 of the manual For best sample analysis all s samples and calibration solutions should be measured at the same temperature Temporal Spatial effects are mainly due to differences in Measured Chlorophyli quantum efficiency and cell size between different species of Graph phytoplankton and photosynthetic bacteria For detailed information on chlorophyll analysis please see the reference list below or visit the Turner Designs webpage at Interfering compounds in natural waters derive from sex eral sources The most common interfering compounds include fluorometer com pheophytins chlorophyll and c dissolved organic matter and fluorometer Optical filters with a wider bandpass will Aquafluor User s Manual 24 Aquatluor User s Manual 23
7. directions from Section 3 7 for collecting and downloading data from the Aguafluor Data will automatically appear in the excel spreadsheet BE SURE to save this data BEFORE closing the TD software Real Time Data Transfer each reading To do so Stop data logging see 3 7 1 Follow steps 1 6 of B4 to crate the connection between the Aquafluor and your computer Insert a sample and press the READ button The results will automatically transfer to the active Excel spreadsheet 3 B 6 IDL Troubleshooting Difficulties can arise when parameters are set incorrectly connections with the cable are not tight enough Here are some common problems Box to the left of the COM port is red This means that the COM port is not available Causes Another instrument or program such as palm pilot hot sync could be occupying the port making it unavailable Make sure to close all programs of this type before downloading data The port selected is incorrect Follow step 4 of connecting to choose another COM port All lights are green but no data transferred even though the instrument says All data downloaded The connection between the instrument and the computer is bad Check and tighten the cable connections Make sure both ends of the cable are plugged in tightly be susceptible to more interference than filters with narrower lt bandpass Appendix C n Vivo Chlorophy
8. upon the properties of the fluorescent material the filters used and the path length A nonlinear relationship is seen at very high concentrations where the fluorescence signal does not increase at a con stant rate in comparison to the change in concentration At even higher concentrations fluorescence signal will de crease even though the sample concentrations are greater This effect is known as signal quenching Linearity may be checked by diluting a sample 1 1 or some other convenient ratio be sure to use your matrix blank for the dilutions If the sample is within the linear range the reading will decrease in direct proportion to the dilution If the reading does not decrease in direct proportion to the dilution or if the reading increases the sample is beyond the linear range of your fluorophore Fluorophore conc Aquafluor User s Manual Te 14 MM PSs A 5 43 Temperature Considerations Fluorescence is temperature sensitive As the temperature of accuracy read blank standard and samples at the same j temperature 44 Positioning Samples iem For low concentration samples cuvettes often will give slightly different measurements depending upon their orientation in the sample compartment This is due to defects in the shape of the cuvette that are not visible to the human eye We recommend that the cuvette be marked at the top and positioned in the sample compartm
9. ent the same wav each time to minimize error 5 We have found that turbidity is particularly sensitive to this factor We recommend for best results using high quality polystyrene cuvettes P N 7000 957 which showed little orientation and cuvette to cuvette variation in testing 5 Data Quality The Aguafluor is only as accurate as the standards that are used to calibrate it This is why it is important to take care when preparing standards samples and blank One should follow good laboratorv practices when preparing all solutions and reagents Aquatluor User s Manual the sample increases the fluorescence decreases For greatestm 5 Warranty 5 1 Terms Turner Designs warrants the Aquafluor Fluorometer and accessories to be free from defects in materials and workman ship under normal use and service for a period of one year from the time of initial purchase with the fo llowing restric tions The instrument and accessories must be installed powered and operated in compliance with the directions in this Aquafluor User s Manual and directions accompanying the accessories 2 Damage incurred in shipping is not covered 3 Damage resulting from measurement of samples found to be incompatible with the materials used in the sample system is not covered 4 Damage resulting from contact with corrosive materials or atmosphere is not covered
10. ers outside of the contiguous continental United States and who have purchased our equipment from one of our authorized distributors contact the distributor If you have purchased direct contact us We will repair the instrument at no charge but we will not pay for shipment documentation etc These charges will be billed at cost NOTE Under no conditions should the instrument or accessories be returned without notice Prior correspon dence ts needed Toensure that the problem is not a trivial one easily handled in your laboratory with consequent savings to everyone b Tospecifically determine the nature of the problem so that repair can be rapid with particular attention paid to the defect you have noted Aquafluor User s Manual 7 A 115 7uiment pecirnicauons Al General Specifications Size Meets IP 67 Standard dustproof and waterproof 41 104 F 5 40 Photodiodes measurement apability from 300 1000nm Low battery circuit failure High blank 10mm x 0mm 1 plastic Automatic Power Down 5 seconds After 90 seconds of inactiv ity A2 Optical and Application Specifications Chlorophyll eem Turbidity Channel Rhodamine Channel Excitation Optics 665nm 800 ppb Temperature Acc C coefficients Linear Emission Optics Limit of Detection Max range i 026 C Exponential Aquafluor
11. es from the calibration blank and standard 4 Press lt ESC gt when finished to return to the Home screen Aquafluor User s Manual 2 4 General Considerations for Analvsis 4 1 Handling Samples l Take care not to spill samples into the sample chamber Wipe up any spills promptly p The Aguafluor is very sensitive and even small amounts of material from a previous sample mav result in errors Use clean cuvette for all readings Thorough and proper cleaning of cuvettes between sample readings is essential and is especially important if you are using the same cuvette for samples and blank 3 the cuvette at least 50 full 2mls Significant error can result if the cuvette does not contain this minimum volume 4 The cuvette MUST BE DRY on the outside when taking readings Moisture and condensation on the outside can result in error Minute bubbles in samples will cause drifting readings Take care not to introduce bubbles into samples Slight tapping with your finger on the outside cuvette wall will often help dissipate bubbles 42 Linear Range and Quenching Fluorescence The linear range is the concentration range in which the readout of the Aquafluor is directly proportional to the concentration of the fluorophore The linear range begins with the smallest detectable concentration and spans to an Aquafluor User s Manual upper limit concentration that is dependent
12. he screw The panel has an o ring which creates a watertight seal The battery panel may be difficult to install if there is no Aquatluor User s Manual Aquatluor User s Manual 4 1 2 Quick View Diagrams lubrication on the o ring Use a silicon based o ring grease to lubricate the o ring if necessary 1 3 General Information and Precautions e The sample compartment cannot accept glass or quartz cuvettes e Aminimum volume of 2mls ina 10x10 cuvette is required for best results e Avoid having any air bubbles in your sample They can significantly affect the fluorescent reading e For best results measuring low turbidities use good polystyrene cuvettes P N 7000 957 See Section 4 4 for further information Aquafluor User s Manual rn Aguafluor h pom m we ec mo al QT 29 a f r i eM t 14 LN k E d mm PETTEE Tes Aquafluor User s Manual 3 Instrument Parameters 3 1 Firmware Flowchart Indicates which channel is active Y Press lt A B gt to toggle between 0 XXXAX and XXX N Press STD VAL Cal Val XXX X lt UPIDOWN gt Turner Designs TD HHIR 0500 5 5 second warm up XXX X d Home Screen Preis lt CAL gt Calib l
13. ll a The detection of in vivo chlorophyll a is by nature a In spite of these concerns this does not mean that gis chlorophyll concentrations cannot be extrapolated f in qualitative measurement Physiological environmental vivo data A simple way correlating in vivo e ee chlorophyll concentrations is accomplished by FeO collecting grab samples for chlorophyll extraction Several samples should be collected within each niche or environment morphological and temporal factors all contribute to the variation between the in vivo signal and the actual chloro phyll concentration of a sample Physiological effects stem rom the change in fluorescence per unit chlorophyll of cells At the time of collection the in vivo value must be noted Once the chlorophyll concentration has been determined through extraction the concentration should be correlated with the at varying physiological states On a basic level an healthy cell will fluoresce more than a healthy cell due to the light energy absorbed is channeled into photosynthesis However in natural assemblages of phytoplankton there is corresponding vivo value see Graph C1 normally a mix of species at varying degrees of health thus Calibration of the Fluorometer averaging out the phy siological effect 101 milbgrarms per cubic mete 40 AN Environmental effects derive from mainly two factors light
14. lorophyll RWT Rhodamine WT TRB Turbidity Calibration Standard Value Before performing a calibration set the value of your standard Press the lt STD VAL gt button Use the up and down arrows to adjust the standard value Holding either arrow button down will activate faster scrolling When finished Press lt ESC gt or lt ENT gt to accept the value and to return to the Home screen Calibration We recommend that you always calibrate before performing any sample analysis After the initial calibration the Aquafluor s solid standard can be used to check for instru ment drift and recalibration adt wi 3 6 Bm Press the CAL button Press ENT to start the calibration Insert your blank and press ENT The AquafluorTM will average the fluorescence for 10 seconds Insert the calibration standard and press ENT Press ENT when the calibration is complete to accept the calibration If ENT is not pressed within 10 seconds you will be asked if you want to abort the calibration Press the up down arrow to abort or accept the calibration respec tively It at anytime during steps 1 4 you want to stop the calibra uon press lt ESC gt This will return you to the Home screen and will default the instrument to the previous calibration Sample Analvsis Insert your sample Press either READ button The instrument will autorange then measure and average the fluorescence
15. t ENT gt to start Insert Blank then press ENT Y Reading Blank XXX Insert Cal Soln then press lt ENT gt Reading Cal 5oln XXX e Calibration Completed ENT lf lt ENT gt is not pressed within 5 seconds this screen will appear Fluare weenie reading Indicates activity of IDL v Pres lt READ gt READING v WAIT XXX X A Press gt then lt ENT gt to toggle between Logging Points Left and TFS Bik NAA FS STD NANA Press DATA Datalogger lt DATA gt l toggle v v v Press eDATA IX Presi lt DATA gt 2X Press lt DATA gt 3X Simui Stop Download data Erase Data lt ENT gt to toggle SX lt ENT gt to SX lt ENT gt to lt ENT gt Y 502101 Logging Download data Erate Data ENT to toggle All downloaded All data crased If there is no data logged this icreen will appear Data logget hai no valid data Aquafluor M User s Manual aed Instrument Power Up To turn on the Aguafluor press the lt ON OFF gt button The instrument takes 5 seconds to warm After the warm up the Aquafluor is ready for operation Fluorescence or Turbidity Channel Choose the appropriate channel for your analysis To do this press the lt A B gt button to toggle between the 2 channels The activated channel will be displayed in the lower left corner of the Home screen CHL Ch
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