Storm™ - GE Healthcare Life Sciences
Contents
1. Storm Operating Instructions 28 9623 52 AA 25 3 Installation 3 4 Connections 26 Storm Operating Instructions 28 9623 52 AA Operation 4 Operation overview 4 1 4 Operation This chapter provides basic instructions for working with Storm For detailed instructions in English see the Storm User Manual 4 1 Operation overview Principle There are two scanning options for Storm instruments e Storage phosphor screen autoradiography e Fluorescent scanning available for Storm 845 and 865 A workflow for performing a scanning is described in the table below Step Action Instructions in 1 Start the instrument and STORM Section 4 2 Scanner Control 2 Expose Storage phosphor screen Section 4 3 to a sample Storage phosphor screen autoradiography option only 3 Clean the instrument Section 4 4 4 Place fluorescent sample or Section 4 4 Storage phosphor screen in instrument 5 Select scanning mode Section 4 4 6 Set scanning parameters Section 4 4 7 Scan sample or Storage phosphor Section 4 4 screen 8 After scanning procedures Section 4 5 Performing Storage phosphor screen autoradiography is a two step procedure first the Storage phosphor screen is exposed to the sample inside an Exposure cassette and secondly the Storage phosphor screen is scanned in the instrument For information of available Storage phosphor screens and Exposure cassettes see Appendix A Accessories on page2. E dca 61 6 1 Power and communication 61 Mss Teelsel aoc 62 MIJE 62 7 Reference information sssesssesssesssessressessesessesesesessesssse 63 FL 5 ce e eai S A 63 TAE EEEO 0 decade t RAE 63 7 5 Ordering information 63 Storm Operating Instructions 28 9623 52 AA 3 Table of Contents Appendix A Accessories eere esset eene te tntntntne 65 A Storage phosphor screens etttetnnnneens 65 A2 Expos re Cassettes mes menant 65 4 Storm Operating Instructions 28 9623 52 AA Introduction 1 Important user information 1 1 1 Introduction Purpose of the Operating Instructions The Operating Instructions provide you with the instructions needed to handle the Storm system in a safe way Prerequisites In order to operate the Storm system safely and according to the intended purpose the following prerequisites must be met e You should have a general understanding of how the PC and Windows work e You must read the safety instructions in the user documentation You should be acquainted with the use of general laboratory equipment and with handling of biological materials In this chapter This chapter contains important user information and a general description of the Storm and its intended use 11 Important user information Read this before using the Storm All users must rea
3. This chapter provides instructions for routine maintenance and also a maintenance schedule Regular maintenance of the Storm system is essential for reliable results 5 1 General Keep the instrument dry and clean Wipe regularly with a soft damp tissue Let the instrument dry completely before use WARNING A Disconnect power Always disconnect power from the instrument before performing any maintenance task 5 2 User maintenance schedule Interval Instructions reference Periodically Check the instrument for radioactive contamination see Section 5 3 Before and after each scan Clean the instrument see Section 5 3 Check the Storage phosphor screen for contamination see Section 5 4 Clean the Storage phosphor screen see Section 5 4 Clean the Exposure cassette see Section 5 5 Clean the Image eraser see Section 5 6 Storm Operating Instructions 28 9623 52 AA 51 5 Maintenance 5 3 Storm instrument 5 3 Storm instrument Checking for contamination Periodically you should check the glass platen and the surface of the inner lid for contamination from radioactive samples To check the instrument for radioactive contamination follow the instruction below 1 Use a Storage phosphor screen that is erased and free from radioactive contamination Place the white side of the screen on the glass platen of the Storm instrument Leave the screen in the instrument overnight Sc
4. 4 5 Preparations before start Exposure procedure 1 Select the Exposure cassette size and style that matches the storage phosphor screen you want to use with the sample see Appendix A Accessories on page 65 NOTICE Do not put uncovered wet gels in an Exposure cassette 2 Use a damp cloth to clean the grid surface inside the Exposure cassette and remove any radioactive contamination Place the sample on the grid It is recommended to e place the sample A face up on the grid and in the upper left corner at least 1 cm from the edge of the screen B e putthe top of the sample A toward the top end of the cassette e f exposing multiple samples to one screen place the samples close together e lign the lanes and rows of the sample with the edges of the grid so that the scanned image will be straight 4 Makea note of the coordinates of the upper left B and lower right C corners of thearea you want to scan The grid coordinates in the Exposure cassette match the coordinates in the Storm instrument 34 Storm Operating Instructions 28 9623 52 AA Operation 4 Preparations before start 4 3 5 Place a mounted screen A in an Exposure cassette B A a Make sure the clamps on the cassette are rotated out of the way b The white side of the screen should face down toward
5. to the right of the extra dot in section Q is not scanned by the Storm 865 and 845 C e Red excited fluorescence Within the etched outline B on the glass platen when scanning with the Storm 865 D d Torecord multiple samples during the same scan place the samples close together For good quality of results the samples should have the same thickness e Align the lanes and rows of the sample with the edges of the grid on the glass platen so that the scanned image will be straight f Place a sample on the glass platen for sandwich gels see step 49 Hold the sample by the edges or use a clean plastic spatula for gels or forceps for membranes to handle the sample Gently lower the sample onto the glass starting at one edge Do not trap air bubbles under the sample or scratch the glass Note For wet samples squirt a little buffer or distilled water on the glass platen Make sure you wipe off the excess buffer or distilled water Note Do not touch the glass platen or the part of the sample that will be read by the Storm instrument Oil from fingerprints and powder from gloves even thoroughly washed gloves can leave a print that can be detected g Place a sandwich gel on the glass plate Squirt approximately 0 5 to 2 ml of deionized water on the glass plate Hold the sandwich gel by the edges lower the sandwich gel diagonally until the lower right corner of the gel rests on the glass plate Do not trap air bubbles under th
6. 12100 1 Safety of machinery Basic concepts Harmonized with general principles and design 2006 42 EC EN ISO 14121 1 Safety of machinery Principles of Harmonized with 14121 2 risk assessment 2006 42 EC CE marking CE The CE marking and the corresponding Declaration of Conformity is valid for the instrument when it is e used as a stand alone unit or e connected to other CE marked instruments or Storm Operating Instructions 28 9623 52 AA 7 1 Introduction 1 3 The Storm system connected to other products recommended or described in the user documentation and used in the same state as it was delivered from GE Healthcare except for alterations described in the user documentation or explicitly authorized by GE Healthcare Regulatory compliance of connected equipment Any equipment connected to the Storm should meet the safety requirements of EN 61010 1 1EC61010 1 or relevant harmonized standards Within the European Union connected equipment must be CE marked 1 3 The Storm system The Storm hardware components The Storm hardware includes the following components Addi Storm instrument Scans exposed Storage phosphor screens and in most models fluorescently labeled samples Storage phosphor screen Collects the image from radioactive samples Exposure cassette Holds the Storage phosphor screen in a light proof environment Image eraser Erases Storage phosphor screens for
7. 65 When scanning fluorescent samples the sample is placed inside the instrument Storm Operating Instructions 28 9623 52 AA 27 4 Operation 4 2 Starting the instrument and STORM Scanner Control 4 2 Starting the instrument and STORM Scanner Control 1 Press the Power switch A on the lower left side of the instrument a SU A 2 The power indicator light on the top of the instrument turns on and remains red during the self test sequence which takes about 45 seconds 3 The power indicator light then turns green Note After you turn on the Storm instrument wait approximately 15 minutes for the instrument to warm up before you start the first scan 4 Turnon peripherals if present according to the manufacturers instructions 5 Turnon the computer and monitor according to the manufacturer s instructions Note Ifthe computer was already on when you turned on the Storm instrument you must restart the computer 6 Locate and double click the STORM Scanner Control shortcut icon on the desktop or select STORM Scanner Control using the Start menu 7 The STORM Scanner Control window appears 4 5 Preparations before start Sample recommendations General sample handling recommendations e Make sure the sample is free from dust and powder Wear powder free gloves when handling the samples e Dust and powder fluoresce and scatter light This causes artifacts on the images and can interfere with the quant
8. General Electric Company All rights reserved First published Nov 2009 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 28 9623 52 AA 11 2009
9. Use the Blue 450 nm excitation setting with a fluorochrome that has an emission longer than 520 nm Use the Red 635 nm excitation setting with a fluorochrome that has an emission longer than 650 nm Low fluorescent sample handling recommendations For fluorescent samples requiring high sensitivity or highly accurate quantitation the material sources stated in Table 4 1 have been tested and found to have low background Table 4 1 Material recommendations for low fluorescent sample handling Material Recommendation Gels general Background fluorescence contributed by the gel matrix Agarose gels Make sure the agarose is completely dissolved and well mixed increases with gel thickness Always use the thinnest gel practical for your experiment especially for agarose gels Make sure your glass plates are absolutely clean before you pour the gel Grease and fingerprint oils from the plates can stick to the gel surface and attract dust and fluorescent dyes before pouring your gel Also avoid generating bubbles when mixing and pouring Uneven agarose concentration and bubbles affect light scatter and can cause artifacts and interfere with quantitation Polyacrylamide Polyacrylamide gels are usually clear and thin less than 1 mm gels The background contribution from the gel material is generally very low Solvents Spectroscopic grade solvents have the lowest autofluorescence Storm Operating Instructions 28 9
10. not been used for several days 1 Clean and erase the screen Cleaning on page 54 and Preparations before start on page 28 NOTICE Before you erase the screen check the Image eraser for contamination from radioactive samples To clean the Image eraser see Cleaning on page 54 2 Storethe screen in a clean light tight box 3 Provide enough time to register the contamination as an image on the screen 4 Mfter storage scan the screen Section 4 4 and examine the image e If contamination is detected clean and erase the screen again and re check for contamination e fnocontamination appear on the image the screens is ready to use GP and LE screens Use a soft cotton cloth and an intensifying screen cleaner for example Kodak Intensifying Screen Cleaner Follow the manufacturer s instructions Alternatively use a small amount of alcohol and distilled water Do not use a powdered detergent Any undissolved particles can scratch the surface of the screen 1 This cleaning procedure removes dust fingerprints static electricity and mild contamination from radioactive samples NOTICE To avoid damaging the LE screen make sure to dry the screen completely after cleaning the screen using an intensifying screen Cleaner Storm Operating Instructions 28 9623 52 AA Maintenance 5 Exposure cassette 5 5 TR screens Use a gentle gas stream or soft brush to remove any particulate matter from the
11. screen from the glass platen use the small tabs attached to the metal back If your screen does not have tabs or the tabs are damaged contact GE Healthcare Technical Support e Toliftalarge mounted screen pull up on the round tabs attached to the metal plate and then slide a finger under the edge of the metal backing Fluorescent samples e Dispose of the sample using the established procedure in your laboratory Checkthe glass platen and sample lid for contamination and clean the glass platen and sample lid of the Storm instrument see Section 5 5 Storm instrument on page 52 If Storage phosphor screen autoradiography scanning After each scan d Remove the sample from the Exposure cassette and dispose of the sample using the established procedure in your laboratory Storm Operating Instructions 28 9623 52 AA Operation 4 Procedures after scanning 4 5 b Check the Storage phosphor screen for contamination Section Checking for contamination on page 54 C Clean the Storage phosphor screen Section Cleaning on page 54 d Erase the Storage phosphor screen Section Prepare Storage phosphor screen for exposure on page 31 e Check the Image eraser for contamination and clean the Image eraser Section 5 6 Image eraser on page 55 f For Storage of Storage phosphor screens Store screens after cleaning decontamination if required and erasure e Atroom temperature e Away from sources of radiation such as s
12. screen is not possible to decontaminate protect the Image eraser and the screen from possible contamination by placing plastic wrap or polyester film between the Storm Operating Instructions 28 9623 52 AA TR screen and the Image eraser surface 31 4 Operation 4 5 Preparations before start Preparation procedure 1 Clean the screen before exposure see Section 5 4 Storage phosphor screen on page 54 for cleaning instructions 2 Erasethe screen The Image eraser is used to erase any residual signal from screens a Hold the screen by its edges with the white side facing the Image eraser b Place the screen on the Image eraser A and rest the screen on the lip B of the Image eraser C A Imagffraser J PORC NES c To erase two small screens at once suspend an unmounted screen from the clip at the top of the Image eraser C NOTICE o Do not use the clip on a TR screen The clip can damage the surface of the screen d Turn the Image eraserlmage eraser power switch on 0 32 Storm Operating Instructions 28 9623 52 AA Operation 4 Preparations before start 4 3 e Set the Image eraser time by pressing the Time button E e Select Normal position out for standard samples The Normal setting takes approximately 10 minutes e Select Extended position if the background or residual image is high e g the original image contained readings of 104 cou
13. 623 52 AA Operation 4 Preparations before start 4 3 Material Recommendation Membranes Transparency support You should scan a test piece of each type of membrane you plan to use and check that the background is low enough for your purposes To avoid contaminating the glass platen and sample lid you can place a sample such as a membrane between two pieces of transparency material For detailed instructions see the Storm User Manual Prepare Storage phosphor screen for exposure Note This section is valid only when performing Storage phosphor screen autoradiography Recommendations for erasing screens NOTICE surface The Storage phosphor screen consists of a relatively soft matrix Handle the screen on the edges or back only Do not touch the white phosphor NOTICE Wear powder free gloves to avoid contaminating the screen with skin oils or powder NOTICE 000 Do not fold roll or gouge an unmounted screen e To avoid contami nating the screen or the Image eraser make sure that both the screen and Image eraser are free from radioactive contamination before placing the screen on the Image eraser See Sections 5 4 and 5 6 e Erase the screen radiation creates e Erase the screen immediately before exposure as well as after scanning Cosmic background on screens left unused for long periods of time until the recorded signal is fully removed e Because a TR
14. GE Healthcare Storm Operating Instructions Original instructions Table of Contents Table of Contents ME ro mS 5 11 Important user information ettet 5 L2 R gulator information 5 emet ettet 6 13 The Storm system T 4 Control SOMMAIRE tede e a Safety WS tir OS ssa eee nn 2 1 Safety precautions e sisesetnnnnntnttrtetttttetttttts 2 2 Laserlight safely encontre CROIRE OO GUN t MEME nnne 2 4 Emergency procedure 25 Recycling information snnt TRON osa ib CHR Mp nets 23 3 1 Site requirements een 23 3 2 Transport 5 5 Unpacking Le nn teen teet 23 5 4 COMMOGU ONS sciet nn 24 Operati Menere ere E E EER 27 41 Operation OVervieW s ssssssssssssssrsrssesserssessesstssossoesossstssasrtsnreseesseeseeniesseesesseescesress 27 4 2 Starting the instrument and STORM Scanner Control 28 4 5 Preparations before start s 28 44 Performing QSCOFiDIPlg xac 37 4 5 Procedures after scanning tts 48 5 Maintenance en tetro taint nent 51 50 m 51 5 2 User maintenance schedule e 51 5 5 SStOFTDIPDIStEBITDYS DTE ritiene e e eee 52 5 4 Storage phosphor screen 54 5 5 Exposure cassette 55 5 6 Image eraser 55 5 7 Replacement Of fuses nnns 56 5 8 Replacement of bulbs isiin 59 6
15. If you open the sample lid while the scanner is in operation the laser turns off WARNING A Do not perform maintenance or repair the equipment before the interlock switch is in the service position Interlock during service 16 Storm is provide with a mechanically locked interlock switch that deactivates the laser during service Before servicing loosen the locking bolt and lift the locking plate so that the interlock switch is easily accessible Move the interlock switch into the service position see Figure 2 1 and Figure 2 2 Figure 2 2 Interlock with mechanical lock loosened Storm Operating Instructions 28 9623 52 AA Safety instructions 2 Labels 2 3 2 5 Labels Location of labels The illustration below shows the location of labels on the Storm instrument and Image eraser GE Healthcare Bio Sciences AB 751 84 UPPSALA Sweden MODEL STORM 1820 1830 7840 1860 C Ge 100 240V 5A 50 60Hz wu NE OS Ga ood S CAUTION Loser Radiation when open AVOID EXPOSURE TO BEAM CLASS 1 LASER PRODUCT Per IEC 60825 CAUTION DISCONNECT POWER BEFORE SERVICING ATTENTION COUPER LE COURANT AVANT L ENTRETIEN WARNUNG VOR DER WARTUNG VOM NETZ TRENNEN y 3 2 Storm Operating Instructions 28 9623 52 AA 17 2 Safety instructions 2 5 Labels Storm instrument identification label The illustration bel
16. able while 46 Note Keep the sample lid closed while scanning Opening the lid shuts off the light source aborts the scan and saves the data already collected Make sure that the Storage phosphor screen is in place the sample lid is shut and all the parameters are correct in the STORM Scanner Control window Click the Scan button in the STORM Scanner Control window Storm Operating Instructions 28 9623 52 AA Operation 4 Performing a scanning 4 4 3 Save As window appears Save as Look in gt et E3 temp scan file4 gel temp scan file gel File name temp scan file gel Files of type Data gel Cancel a Typeaname for the scan in the File name box STORM Scanner Control creates an image file and stores it using the Data File gel type b Optional Change folder location of the file Default folder is the Data folder Note Saving the scanned data to a folder located on a removable media disk drive can cause a loss of data It is recommended to use a folder on the computer hard drive Move the image file to the removable media after scanning is completed C Click Save to start the scan During the scan the inner lid of the Storm instrument lowers to hold the screen flat against the glass platen of the instrument 4 Monitoring the scan progress After you start the scan the Scan in Progress window appears and the green Scan indicator light o
17. an the screen Section 4 4 e Ifan image that looks like a gel or blot appears in the Scan In Progress window the instrument is contaminated e lfawhite or gray image appears the image was probably created by background contamination and the instrument is not contaminated To decontaminate the instrument follow the instructions below to clean the glass platen Cleaning Clean the glass platen Clean the glass platen according to this instructions before and after you scan each sample Recommendations e Wear powder free gloves to protect your hands and to avoid transferring oils from your hands onto the glass platen e Do not use window cleaners They contain ingredients that can fluoresce e The use of acetone or the excessive use of ethanol can shorten the lifetime of the instrument e Protect the glass from scratches Scratches interfere with accurate imaging and quantitation To clean the glass platen follow the instruction below 52 Storm Operating Instructions 28 9623 52 AA Maintenance 5 Storm instrument 5 3 1 Grasp the lid release Fig 5 1 B under the center front of the sample lid Figure 5 1 A and pull the release forward until the lid opens Raise the lid all the way Figure 5 1 Opening sample lid 2 Optional If the instrument has been used for fluorescent scanning and fluorescent material has come in direct contact with the glass platen moisten a lint free cloth with 1096 hydrogen peroxide and wipe the glas
18. ct operating voltage appears in the fuse window Selecting the wrong voltage can severely damage the Image eraser Storm Operating Instructions 28 9623 52 AA Maintenance 5 Replacement of bulbs 5 8 5 8 Replacement of bulbs The Image eraser contains four warm white compact fluorescent bulbs When any one of the bulbs fails replace all four bulbs to provide uniform light intensity across the screen To order replacement bulbs contact GE Healthcare Technical Support or your local distributor see the manufacturer part number on the bulb See back cover for contact information order replacement bulbs and fixtures from GE Healthcare Technical Support The bulbs shipped with the Image eraser are epoxied to the fixture to avoid damage during shipment The replacement fixture and bulbs are not epoxied NOTICE If you are replacing the bulbs shipped with the Image eraser you must To replace the bulbs follow the instruction below 1 Oo gt Turn off the Image eraser and disconnect the power cord Unscrew the two thumbscrews Fig 5 4 A that hold the clip support Fig 5 4 B and diffuser screen Fig 5 4 C in position Lift off the clip support and diffuser screen Unscrew the bulb and fixture from each receptacle Fig 5 4 D Screw a new bulb and fixture into each receptacle Replace the front diffuser screen and clip support and screw the two thumbscrews Verify in the fuse window that the fuse voltag
19. ctive measures such as wearing protective glasses and gloves resistant to the substances used Follow local and or national regulations for safe operation and maintenance of the system Installing and moving the instrument 14 WARNING Power cord Only use power cords delivered or approved by GE Healthcare WARNING Protective ground The Storm instrument must always be connected to a grounded power outlet WARNING Make sure that the instrument is placed on a stable flat bench with adequate space for ventilation WARNING Access to power switch and power cord The power switch must always be easy to access The power cord must always be easy to disconnect WARNING Supply voltage Make sure that the supply voltage at the wall outlet corresponds to the marking on the instrument before connecting the power cord gt Dm e CAUTION Heavy object The Storm instrument weighs approximately 75 kg Two people are required to lift the system safely Storm Operating Instructions 28 9623 52 AA Safety instructions 2 Safety precautions 2 1 System operation WARNING Electrical shock hazard after spillage If there is a risk that large volumes of spilled liquid may penetrate the casing of the Storm instrument immediately switch off the instrument disconnect the power cord and contact an authorized service engineer WARNING If the cover or doors of the Storm instrument bec
20. d the safety instructions in the Storm user documentation before installing using or maintaining the system Do not operate the Storm system in any other way than described in the user documentation Otherwise you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment Intended use The Storm system is an optical scanner that produces digital images of radioactive of fluorescently labeled samples The Storm system is intended for research use only and shall not be used in any clinical procedures or for diagnostic purposes Storm Operating Instructions 28 9623 52 AA 5 1 Introduction 1 2 Regulatory information Safety notices These Operating Instructions contain WARNINGS CAUTIONS and NOTICES concerning the use of the product with meanings as defined below WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to proceed until all stated conditions are met and clearly understood AN NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Typographical conventions Software items are identified in the text by bold i
21. e the maximum width of the scan area is from section A to the middle of section Q You cannot scan a narrow strip along the right side of the grid C Release the mouse button The scan area you selected appears in white Storm Operating Instructions 28 9623 52 AA 45 4 Operation 44 Performing a scanning 44 4 Set sample orientation a b c d Click the Orientation button in the Set Options area The orientation buttons appear Symbol Sample orientation The top of the sample is face up toward the low numbered end of the Exposure cassette The top of the sample is face up toward the right side of the Exposure cassette The top of the sample is face up toward the high numbered end of the Exposure cassette The top of the sample is face up toward the left side of the Exposure cassette The top of the sample is face down toward the right side of the Exposure cassette The top of the sample is face down toward the high numbered end of the Exposure cassette The top of the sample is face down toward the left side of the Exposure cassette 2 The top of the sample is face down toward the low numbered end of the Exposure cassette Click the button that represents how you placed the sample in the Exposure cassette Storage phosphor screen autoradiography or on the instrument glass platen fluorescent scanning During the scan the STORM Scanne
22. e is correct Connect the power cord to a grounded mains outlet and turn on the Image eraser A Image Eraser B Figure 5 4 Replace bulbs in the Image eraser Storm Operating Instructions 28 9623 52 AA 59 5 Maintenance 5 8 Replacement of bulbs 60 Storm Operating Instructions 28 9623 52 AA 6 Troubleshooting Troubleshooting 6 Power and communication 6 1 This chapter gives a brief guide of how to solve instrument related problems that might occur when using Storm If you are experiencing problems that you cannot correct contact your local service representative Power and communication Error symptom The power indicator lamp does not turn on The instrument is on but cannot communicate with the computer Possible cause The instrument is not plugged in Faulty wall outlet Fuses have blown The computer was turned on before the instrument Corrective action Plug in the instrument Test the outlet or try another one Change the fuses see Section 5 7 Make sure the instrument is turned on and then restart the computer The SCSI cable is not properly plugged into all peripherals Check the connections to make sure they are plugged in and are securely fastened Incorrect or too long SCSI cables Try another SCSI cable that you know is good Not all SCSI cables are constructed the same We recommend that you use only SCSI cables from GE Healt
23. e sample Note Donottouch the part of the sandwich gel that will be read by the Storm instrument Do not touch the glass plate Oil from fingerprints and powder from gloves even thoroughly washed gloves can leave a print that can be detected h Make a note of the coordinates of the upper left and lower right corners of the area you want to scan see step 4b in this instructions for an example This information will be used when setting scanning area 5 Close the sample lid and press it down until the latch clicks The screen or sample is now ready for scanning Storm Operating Instructions 28 9623 52 AA 41 4 Operation 4 4 Performing a scanning 42 Select scan parameters 1 In the Setup Acquisition Mode area of the STORM Scanner Control window click e Storage Phosphor to select Storage phosphor scan acquisition mode e Fluorescence to select fluorescence scan acquisition mode 2 STORM Scanner Control c data temp File Templates Help Scanner Information Modet STORMS65 Address 5 5 erial Number 90000 Template default ON CD 74 C U N IL 13 1 E T SS a Setup User Name Scanners Acquisition Mode Storage Phosphor Fluorescence Setup Pixel size Orientation R 200 microns Pre Options User Comment SCAN Scanning Informati
24. e sample sticks to the screen gently peel off the sample Note To keep unmounted screens from moving and causing a double image press down on one edge of the screen and then lift up the opposite edge of the screen c Protect the screen from direct light and proceed immediately to load the Storage phosphor screen into the scanner 38 Storm Operating Instructions 28 9623 52 AA Operation 4 Performing a scanning 4 4 d Load the Storage phosphor screen A on the instrument glass platen B with the white phosphor side of the screen face down Align the upper left corner C of e Unmounted large or small screens Align the top and left edges with the back and left etched lines D on the glass platen e Mounted large screens Align the top and left edges of the metal plate with the inner edge of the black grid labels at the back and left edges of the glass platen E e Mounted small screen Insert the L shaped adapter by aligning the outer edge of the adapter with the inner edge of the black grid labels at the back and left edges of the glass platen E Next align the top and left edges of the metal plate with the inner edges of the adapter e Gently lower the screen on the glass platen Note An unmounted screen may not lie completely flat against the glass When you initiate the scan the instrument lowers the inner section of the lid to hold the screen flat Storm Operating Instructions 28 9623 52 AA 39 4 O
25. erlocks on the sample lid or try to gain access to the interior of the instrument through any other opening Exposure to laser light can cause injury Viewing the laser light directly can cause blindness Storm Operating Instructions 28 9623 52 AA Safety instructions 2 Labels 2 3 Image eraser identification label The illustration below shows an example of the serial number certification label that is attached to the Storm instrument The label is located on the right side of the Image Eraser GE Healthcare Bio Sciences AB 751 84 UPPSALA Sweden MODEL 810 UNV 100 120 220 240V 1 6A 50 60Hz dits C N12406 unes A A VY T L6AL 250V Symbols used in safety labels Label Description Warning Read the user documentation before using the system Do not open any covers or replace parts unless specifically stated in the user documentation Indicates that hazardous voltages are generated inside the Thi instrument The system complies with the requirements for electromagnetic compliance EMC in Australia and New Zealand C The system complies with applicable European directives Storm Operating Instructions 28 9623 52 AA 19 2 Safety instructions 2 4 Emergency procedure Labels concerning use of hazardous substances Label Description This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal wa
26. hcare The SCSI bus is not properly terminated Add the terminator as discussed in Section 3 4 Storm Operating Instructions 28 9623 52 AA 61 6 Troubleshooting 6 2 Scanning 6 2 Scanning Error symptom Possible cause Corrective action The instrument does The sample lid is not Make sure you press the lid all not scan and a completely closed the way down until the latch warning message clicks displays stating that the sample lid is up During a scan the The computer has Restart the computer using the Scan in Progress stopped functioning manufacturer s instructions window remains Then open the STORM Scanner unchanged for at least Control reselect the scanner three minutes and the parameters and start the scan pointer does not again respond when you move the mouse The software aborts a PC communication Restart the scan as follows scan and either failed 1 Close the STORM Scanner The software Control window Leave the displays the computer on message Scan peed 2 Turnoffthe power switch on the back of the Storm hardware error instrument Wait a few please try to scan i seconds and turn it on again ora message that does not give 3 Open the STORM Scanner instructions for Control software reselect resolving the the parameters and rescan problem If the problem occurs e The red scan repeatedly contact GE indicator light Healthcare Technical blink
27. ight of the power switch Storm instrument on page 10 To change a fuse in the Storm instrument follow the instruction below 56 Storm Operating Instructions 28 9623 52 AA Maintenance 5 Replacement of fuses 5 7 1 Turn off the Storm instrument and disconnect the power cord A GE E Figure 5 2 Opening the fuse box of the Storm instrument 2 Place the end of the small flathead screwdriver into the small groove Fig 5 2 A to the right of the fuse window Fig 5 2 B and pry to open the fuse box 3 Placethe screwdriver in the small groove Fig 5 2 C to the right of the fuse holder Fig 5 2 D and pry out the fuse holder When the holder is loose pull it out of the fuse box 4 One or both fuses might be blown Replace the blown fusels with new fuse s of the same type and rating Fuse specifications are listed in Section 7 1 and on the label located on the underside of the sample lid 5 Insert the fuse holder into the fuse box 6 Snap the cover of the fuse box back into place 7 Reconnect the power cord and turn on the Storm instrument Changing fuses in the Image eraser You can use the Image eraser with either a 220 240 V 230 V power source or a 100 120 V 115 V power source The selected operating voltage of the Image eraser appears in the fuse window on the right side of the Image eraser next to the power switch Image eraser on page 11 To change a fuse in the Image eraser follow the
28. instruction below 1 Turn off the Image eraser and disconnect the power cord Storm Operating Instructions 28 9623 52 AA 57 B 5 7 58 Maintenance Replacement of fuses 2 Note the voltage 115 V or 230 V showing in the fuse window Fig 5 3 A Figure 5 3 Opening the fuse box of the Image eraser Place the end of a small flathead screwdriver in the small groove Fig 5 3 B above the fuse window and pry to open the fuse box Place the screwdriver in the groove Fig 5 3 C on the upper edge of the fuse holder Fig 5 3 D and pry out the fuse holder When the holder is loose pull it out of the fuse box One or both fuses might be blown Replace the blown fuse s with new fusels of the same type and rating Fuse specifications are listed in Section 7 1 and on the label located on the unit Insert the fuse holder into the fuse box making sure the correct voltage rating for your power supply is right side up Snap the cover of the fuse box back into place Check that the correct voltage rating for your power source appears in the voltage window If the voltage is incorrect for your power source remove the fuse holder rotate the holder 180 so that the correct voltage rating is right side up and reinsert the fuse holder Reconnect the power cord to a grounded mains outlet and turn on the Image eraser NOTICE o Before you turn on the Image eraser after you change the fuse make sure that the corre
29. ired to lift the system safely Before moving the instrument 1 Inthe STORM Scanner Control window choose Park Head and Exit from the File menu 2 Turn off the instrument computer and peripheral devices 3 Disconnect the power cords and the SCSI connection s 4 Graspthe metal bottom of the system firmly 3 3 Unpacking e the equipment for any apparent damage before starting installation e Document any damage carefully and contact your GE Healthcare representative Storm Operating Instructions 28 9623 52 AA 23 3 Installation 3 4 Connections 3 4 Connections Removing SCSI cover On the Storm instrument grasp the two forward extensions of the SCSI cover pull upward and then remove the cover Description 1 SCSI cover 2 SCSI connector Communication Connect a SCSI cable between the computer and the lower Storm SCSI port Add a terminator after the last device in the SCSI chain NOTICE Make sure the total length of all the SCSI cables including the internal cables does not exceed 6 m The cable length inside the instrument is 0 3 m 24 Storm Operating Instructions 28 9623 52 AA Installation 3 Connections 3 4 Electrical power WARNING A Power cord Only use power cords delivered or approved by GE Healthcare Connect the power cords of the Storm instrument and Image eraser to grounded power outlets specified in Chapter 3 1 Site requirements on page 23
30. itation 28 Storm Operating Instructions 28 9623 52 AA Operation 4 Preparations before start 4 3 Storage phosphor screen sample handling recommendations NOTICE Do not use wet gels in combination with tritium TR screens NOTICE Do not directly expose the Storage phosphor screen to wet chemicals of any kind especially not to organic solvents Since organic solvents penetrate plastic plastic wrap will not protect the Storage phosphor screens from organic solutions NOTICE Neutralize alkaline denaturing gels and make sure the sample is free from acetic acid vapors and organic solvents These materials are harmful to the Storage phosphor screens and can penetrate plastic wrapping Protect a general purpose GP screen from contamination by separating a wet gel from the screen with a piece of plastic wrap or polyester film Wrap the plastic wrap completely around the gel so that liquid cannot leak out This precaution reduces screen contamination with minimum attenuation of the signal e Do not use scintillants or enhancers such as EN3HANCE and Amplify These products interfere with the proper function of the screen Fluorescent sample handling recommendations e Always rinse the gloves with distilled or tap water before handling the sample and before preparing the reagent e Handle filter solutions as follows e Remove dust particles by filtering all the stock solutio
31. l Enter user comments User Comment If desired comments to the scan can be entered in the User Comment box in the Setup area The comments are for reference only and do not affect the scan Storm Operating Instructions 28 9623 52 AA 45 4 Operation 4 4 Performing a scanning 10 11 The Scanning Information area displays the approximate image file size the approximate scan time and the number of lines per channel Scanning Information Total Scan Time 7 Min 0 Sec Channel Excitation Emission Sensitivity Lines Per Channel 1500 1 Red 635rm 3308P Image Size per Channel 282 MB STORM Scanner Control saves the scan parameters with the image You can view but not change these parameters in analyzing software Optional Use a template Using a template is a quick way to retrieve the scan parameters you use frequently The template contains the scan parameters for the selected instrument which include the grid area scan acquisition mode sample orientation pixel size and image analysis software Comments in the User Comment box are not saved with the template See Storm User Manual for instructions how to create and use a template Close the sample lid and press it down until the latch clicks The sample or screen is ready to scan Starting the scan scanning You can severely damage the internal mechanism of the instrument NOTICE Never turn off the Storm instrument or disconnect the SCSI c
32. mately one tenth of the exposure time of an x ray film when exposing Storage phosphor screens 36 Storm Operating Instructions 28 9623 52 AA Operation 4 Performing ascanning 4 4 4 4 Performing a scanning Note Ifradioactive samples are used the instrument should periodically be checked for contamination from radioactive samples Check the glass platen and the surface of the inner lid according to Section Checking for contamination on page 52 Load sample or Storage phosphor screen to instrument 1 Open the sample lid A by grasping the lid release under the center front of the sample lid B and pull the release forward until the lid opens Raise the lid all the way 2 Inspect and clean of the instrument before use d Cleanthe glass platens before and after every scanning see Section Cleaning on page 52 for instructions b Inspect and if required clean the sample lid inner surface according to Section Cleaning on page 52 Storm Operating Instructions 28 9623 52 AA 37 4 Operation 44 Performing a scanning 3 If performing Storage phosphor screen autoradiography Place a screen in the instrument a Open the latch on the Exposure cassette e For mounted screens Flip the lever s on the cassette and then rotate the clamp s clockwise so that they no longer cover the screen E 3 e For unmounted screens Open the latch and raise the lid of the cassette b Remove the Storage phosphor screen If th
33. n the top of the instrument blinks Asthe instrument scans the sample or screen STORM Scanner Control displays the image in the Scan in Progress window and additional information e To abort the scan click Cancel Scan in the Scan in Progress window A message appears asking if the data file should be deleted Storm Operating Instructions 28 9623 52 AA 47 4 Operation Procedures after scanning 45 4 5 Procedures after scanning After scanning procedures 48 After scanning 1 STORM Scanner Control saves the image using the file name you selected in the Save As window The Scan in Progress window displays a Complete message Saturated data appear in red in the Scan in Progress window If the image appears too saturated you might not be able to analyze the image correctly Note Before repeating the scan verify in analyzing software that the image is not too saturated to analyze Note Ifscanning a Storage phosphor screen use a clean Storage phosphor screen to re expose the original sample or to a new sample since the scanning process destroys the signal on the used Storage phosphor screen To avoid saturation of the data expose the screen for a shorter time The Scan indicator light on the Storm instrument turns off Open the sample lid on the instrument Remove the fluorescent sample or Storage phosphor screen Storage phosphor screens e Avoid touching the white side of the screen e To pick up a large mounted
34. ns used to prepare the sample sample matrix and buffers e Use clean rinsed containers e When diluting clean reagents to working concentration use distilled water collected in a rinsed container so that you do not have to filter the solutions again e If available use dust free reagents such as fluorescent labels They require no further filtration e Avoid fluorescent indicator dyes Many of the commonly used electrophoresis tracking dyes fluoresce Whenever possible put the tracking dye in a separate lane Alternatively dilute the indicator dyes as much as possible Storm Operating Instructions 28 9623 52 AA 29 4 Operation Preparations before start 4 5 30 Avoid excessive exposure to light Fluorochromes differ greatly in their sensitivity to light induced degradation photobleaching When working with sensitive fluorochromes and fluorescently labeled samples use low light conditions Recommendation for selecting the label for the fluorescence scanning Blue excited fluorescence Detects samples labeled with fluorochromes that have emissions longer than 520 nm Use the Blue 450 nm excitation setting on the Storm 865 and 845 Red excited fluorescence Detects samples labeled with fluorochromes that have emissions longer than 650 nm Use the Red 635 nm excitation setting on the Storm 865 Dual label fluorescence On the Storm 865 you can label the sample with two fluorochromes and create a multichannel image
35. nts and higher The Extended setting takes approximately 20 minutes f Press the Erase button Check for contamination of the screen between exposures or if the screen has not been used for several days a Clean and erase the screen as described above b Store the screen in a clean light tight box c Provide enough time to register the contamination as an image on the screen d After storage scan the screen see Section 4 4 Performing a scanning on page 37 and examine the image e If contamination is still present clean and erase the screen again and re check for contamination e f no contamination appear on the image the screens is ready to use Storage phosphor screen exposure Note This section is valid only when performing Storage phosphor screen autoradiography Recommendations for storage phosphor screen exposures Use an Exposure cassette to expose the screen to the sample Expose thick samples such as wet gels or TLC plates which are too thick to fit in an Exposure cassette together with a screen in a light tight drawer or similar To expose multiple samples on the same screen make sure the samples have the same thickness It is important to place the screen on the sample correctly the first time Adjusting the position of the screen can result in a double exposure If you must adjust the position remove the screen and erase it Storm Operating Instructions 28 9623 52 AA 33 4 Operation
36. ome damaged do not continue to use the instrument Contact an authorized service engineer WARNING Do not attempt to defeat the safety interlocks on the sample lid or try to gain access to the interior of the instrument through any other opening Exposure to laser light can cause injury For example viewing the laser light directly can cause blindness CAUTION Class 3B Laser radiation when open and interlock defeated Avoid exposure to the beam Maintenance WARNING Electrical shock hazard All repairs should be done by service personnel authorized by GE Healthcare Do not open any covers or replace parts unless specifically stated in the user documentation WARNING Disconnect power Always disconnect power from the instrument before performing any maintenance task WARNING Do not perform maintenance or repair the equipment before the interlock switch is in the service position PPP P bb E Storm Operating Instructions 28 9623 52 AA 15 2 Safety instructions 2 2 Laser light safety 2 2 Laser light safety General The Storm instrument is a Class laser instrument that houses a Class IIIB laser and under the specified operating procedures does not allow operator exposure to laser light The laser with power up to 5 mW is accessible in the interior of the instrument The safety interlocks in the Storm instrument are designed to prevent you from being exposed to the laser beam
37. on Total Scan Time 7 Min 0 Sec Lines Per Channel 1500 Image Size per Channel 2 82 MB Channel Excitation Emission Sensitivity PMT 1 Red 535nm 390 BP The grid in the STORM Scanner Control window allows you to select the scan area The letter and number markings correspond to the markings in the Exposure cassette and on the glass platen of the Storm instrument The white rectangle on the grid designates the area to scan Use either the current scan area or select a new one Storm Operating Instructions 28 9623 52 AA Operation 4 Performing a scanning 4 4 ns Information Model STORM855 Address 5 Serial Number 90000 g m Setup User Name Scanners r Acquisition Mode 34 Storage Phosphor Fluorescence Setup Options Pixel size Orientation R 200 microns Press Sample User Comment vj SCAN Channel Excitation Emission Sensitivity Total Scan Time 7 Mn 0 Sec Lines Per Channel 1500 1 Red 635 330BP Image Size per Channel 2 82 MB 3 Toselect a new scan area a Place the pointer in the grid square corresponding to the upper left corner of the area you want to scan A1 b Drag the pointer to the grid square corresponding to the lower right corner of the area you want to scan J15 in this example Note For blue excited fluorescenc
38. ons 28 9623 52 AA 11 1 Introduction 14 Control software 12 Storm Operating Instructions 28 9623 52 AA Safety instructions 2 Safety precautions 2 1 2 Safety instructions This chapter describes safety precautions safety labels emergency procedures and decommissioning information for Storm 2 1 Safety precautions Introduction Before installing operating or maintaining the system you must be aware of the hazards described in the user documentation Follow the instructions provided to avoid personal injury or damage to the equipment General precautions WARNING Do not operate the Storm instrument in any other way than described in the Storm manuals WARNING Operation and user maintenance of the Storm instrument should be performed by properly trained personnel only WARNING Do not use any accessories not supplied or recommended by GE Healthcare oP gt NOTICE This is a Class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures Personal protection A WARNING Always use appropriate personal protective equipment during operation and maintenance of Storm system Storm Operating Instructions 28 9623 52 AA 13 2 21 Safety instructions Safety precautions gt WARNING Hazardous substances When using hazardous chemical and biological agents take all suitable prote
39. ow shows an example of the serial number certification label that is attached to the Storm instrument The label is located on the underside on the sample lid 28 9328 12 Storm 825 Serial No 100 240V 5A 50 60Hz 1234574 F SA L 250V 6 Hue Manufactured 20 Month Year0108 This product conforms to the applicable requirements of 21 CFR subchapter J at the date of manufacture Made in Sweden GE Healthcare Bio Sciences AB 751 84 Uppsala Sweden 9 2893429400 Laser light warning labels The labels shown below warn of exposure to laser light They are located on the main cover of the Storm instrument under the SCSI cover Label Description 18 CLASS 1 LASER PRODUCT Per IEC 60825 The Storm instrument conforms to the international standard IEC 60825 CAUTION Laser Radiation when open AVOID EXPOSURE TO BEAM CAUTION Avoid exposure to the laser beam when the lid is open CAUTION CLASS 38 LASER RADIATION WHEN OPEN AND INTERLOCK DEFEATED AVOID EXPOSURE TO THE BEAM VORSICHT LASERSTRAHLUNG KLASSE 3B WENN ABDECKUNG GEOFFNET UND SICHERHEITSVERRIEGELUNG UBERBRUCKT NICHT DEM STRAHL AUSSETZEN ATTENTION RAYONNEMENT LASER DE CLASSE 38 QUAND ALLUM ET VERROUILLAGE ENLEV VITEZ TOUTE EXPOSITION AU FAISCEAU CAUTION Class 38 Laser product when open and interlock defeated Avoid exposure to the beam Do not attempt to defeat the safety int
40. peration 4 4 Performing a scanning 4 If performing fluorescent scanning Place a sample in the instrument Note Make sure you place the sample in the correct position when you first set it down Any fluorescent material left on the glass platen when you move the sample can result in a double image Before you load a sample determine how to position it on the glass platen d Sample orientation Determine whether to place the sample face up or face down on the glass platen The Storm system illuminates the sample and collects data from underneath the sample Make a note of the orientation of the sample e Foraone sided opaque sample such as a membrane or TLC plate place the sample face down e Foratransparent sample such as a polyacrylamide gel place the sample either face up or face down e Ifthe sample is physically uneven on one side such as an agarose gel place the smooth side down This allows the sample to lie flat b Sample position C E The sample A should be positioned near the upper left corner of the scan area Each lettered or numbered grid segment is marked by two dots For this example the grid coordinates are A1 and J13 40 Storm Operating Instructions 28 9623 52 AA Operation 4 Performing a scanning 4 4 c Maximum available scan area for e Blue excited fluorescence Within the etched outline B on the glass platen except that the narrow segment approximately 3 cm
41. phor screens detect the beta and gamma ionizing radiation from most isotopes There are three types of Storage phosphor screens available e General purpose GP storage phosphor screen e Tritium TR storage phosphor screen Table A 1 Available sizes and types of storage phosphor screens Screen size Screen type cm General purpose GP Tritium TR 19 x 24 Not available Mounted unmounted 20 x 25 small screen Mounted unmounted Not available 35 x 43 large screen Mounted unmounted Not available A 2 Exposure cassettes In general the Storage phosphor screens are exposed to a sample in an Exposure cassette Four styles of Exposure cassettes are available Small cassette for small mounted screens e Small cassette for small unmounted screens e Large cassette for large mounted screens e Large cassette for large unmounted screens Storm Operating Instructions 28 9623 52 AA 65 Appendix A Accessories A 2 Exposure cassettes 66 Storm Operating Instructions 28 9623 52 AA For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com quantitative_imaging imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company Storm is a trademark of GE Healthcare companies All third party trademarks are the property of their respective owners 2009
42. r Control maps the pixels to display the image of your sample face up and top end up Storm Operating Instructions 28 9623 52 AA Operation 4 Performing ascanning 4 4 Select pixel size Options Pixel size Orientation R 200 microns F Choose the pixel size from the Pixel size list in the Setup Options area e For most standard electrophoresis samples choose 200 microns which provides the fastest scan time and the smallest image file size The 200 um pixel size produces 50 data points per cm and 100 data lines per grid square e For samples requiring high resolution such as DNA sequencing choose 100 microns The 100 um pixel size produces 100 data points per cm and 200 data lines per grid square e For samples requiring very high resolution such as whole body autoradiography choose 50 microns The 50 um pixel size produces 200 data points per cm and 400 data lines per grid square Optional for fluorescent samples Select press sample parameter Tickthe Press Sample check box if a sample that does not lie flat against the glass platen of the instrument such as a dry membrane or filter paper is to be scanned The inner lid of the Storm instrument will go down to press the sample flat Note Donotselect Press Sample if you are scanning wet membranes wet gels or soft samples Pressing wet or soft samples can damage the instrument In addition pressing a soft sample can distort the scanned image Optiona
43. raser and disconnect the power cord Storm Operating Instructions 28 9623 52 AA 55 5 Maintenance 5 7 Replacement of fuses 2 Clean the surface of the Image eraser with a damp cloth moistened with a solution of totally rinsable laboratory detergent Do not use powdered detergents Any undissolved particles can scratch the surface of the Image eraser NOTICE Use liquid sparingly when cleaning excess liquid may leak into the equipment and damage it Use damp cloth only do not pour or spray liquid onto the equipment 3 Verify that the Image eraser surface is free from radioactive contamination 4 Reconnect the power cord to a grounded mains outlet and turn on the Image eraser 5 7 Replacement of fuses General WARNING A Disconnect power Always disconnect power from the instrument before replacing fuses Refer to Section 7 1 Specifications on page 63 for information about the fuse types and rating WARNING If a fuse requires repeated replacement do not continue to use the instrument Contact an authorized service engineer For continued protection from fire hazard replace only with same type and rating of fuse i WARNING Changing fuses in the Storm instrument You can use the Storm instrument with either a 220 240 V 230 V power source or a 100 120 V 115 V power source The power supply in the instrument switches to the correct voltage automatically The fuse box is located to the r
44. re decommissioning and all local regulations shall be followed with regard to scrapping of the equipment Disposal general instructions When taking Storm out of service the different materials must be separated and recycled according to national and local environmental regulations Recycling of hazardous substances Storm instrument contains hazardous substances Detailed information is available from your GE Healthcare representative Disposal of electrical components Waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment Storm Operating Instructions 28 9623 52 AA 21 2 Safety instructions 2 5 Recycling information 22 Storm Operating Instructions 28 9623 52 AA Installation 3 Site requirements 3 1 3 Installation This chapter provides information regarding installation of the Storm system For detailed information on installation see Storm Installation Guide 3 1 Site requirements Parameter Requirement Power supply 15 A 100 120 V or 10 A 220 240 V Line frequency 50 to 60 Hz Ambient temperature 15 C to 35 C Placement Stable laboratory bench Humidity 10 to 95 non condensing 3 2 Transport CAUTION Heavy object The Storm instrument weighs approximately 75 kg Two people are requ
45. reuse tional accessories include a SCSI cable a SCSI terminator power cords and tools The Storm instrument connects to a computer running the Microsoft Windows operating system Storm Operating Instructions 28 9623 52 AA Introduction 1 The Storm system 1 3 Description 1 Storm instrument 2 Image eraser 3 Exposure cassettes for mounted screens 4 Exposure cassettes for unmounted screens 5 Mounted Storage phosphor screen 6 Unmounted Storage phosphor screen Storm Operating Instructions 28 9623 52 AA 9 1 Introduction 13 The Storm system Storm instrument 10 The Storm system is an optical scanner that produces digital images of radioactive or fluorescently labeled samples 4 Figure 1 1 Main parts of the Storm instrument 5 No Description 1 Back 2 Front 3 Sample lid 4 Power switch 5 Fuse holder Storm Operating Instructions 28 9623 52 AA Introduction 1 Control software 1 4 Image eraser The Image eraser is used to erase Storage phosphor screens for reuse ImageEraser 1 Figure 1 2 Main parts of Image eraser No Description 1 Front view 2 Side view 3 Fuse holder 4 Power switch 1 4 Control software STORM Scanner Control software is a complete software for control and supervision of the Storm system The software runs under the Microsoft Windows operating system Storm Operating Instructi
46. s Support 6 5 Image For troubleshooting regarding image related problems please refer to Storm User Manual 62 Storm Operating Instructions 28 9623 52 AA Reference information 7 Specifications 7 1 7 Reference information 7 1 Specifications Parameter Value Ingression protection IP 20 Supply voltage 15 100 120 VAC or 10 A 220 240 VAC 50 to 60 Hz Power consumption Storm instrument 500W Power consumption Image eraser lt 150 W Fuse specification Storm instrument F5AL250V Fuse specification Image eraser T 1 6A L 250V Dimensions Storm instrument h x w x d cm 42 x 76 x 76 Dimensions Image eraser h x w x d cm 63 5 x 42 x 32 5 Weight Storm instrument kg 75 Weight Image eraser kg 20 Acoustic noise level lt 70dBA Ambient temperature 15 C to 35 C Relative humidity tolerance 10 to 95 non condensing 7 2 Literature For further information regarding the Storm refer to the following e Storm User Manual e Storm Installation Guide e Storm Contents and Unpacking Instructions 7 5 Ordering information For ordering information visit www gelifesciences com quantitative imaging Storm Operating Instructions 28 9623 52 AA 63 7 Reference information 7 3 Ordering information 64 Storm Operating Instructions 28 9623 52 AA Accessories Appendix A Storage phosphor screens A 1 Appendix A Accessories A 1 Storage phosphor screens Storage phos
47. s several times 3 Clean the glass with distilled water and a clean lint free cloth or paper If visible spots remain clean the glass first with 7596 ethanol and then with distilled water Clean the sample lid Under normal circumstances the surface of the inner lid should not come in contact with contaminants However it is good laboratory practice to check the surface periodically for contamination For example you can perform a wipe test or use the procedure that you used to check the glass platen Checking for contamination on page 52 except place the screen in the instrument with the phosphor side up If necessary clean the surface with a damp not saturated cloth moistened with a small amount of distilled water If visible spots remain clean the surface first with 7596 ethanol and then with distilled water NOTICE Use liquid sparingly when cleaning excess liquid may leak into the equipment and damage it Use damp cloth only do not pour or spray liquid onto the equipment Note Weor gloves to protect your hands when cleaning the sample lid using 7596 ethanol Note To prevent liquid from seeping inside the lid or rolling down onto the glass platen do not spray liquid on the lid Storm Operating Instructions 28 9623 52 AA 53 5 Maintenance 5 4 Storage phosphor screen 54 Storage phosphor screen Checking for contamination Cleaning 54 Recommended to perform between exposures or if the screen has
48. ste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment This symbol indicates that the product contains hazardous materials in excess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronics 2 4 Emergency procedure This section describes how to do an emergency shutdown of the Storm instrument This section also describes the result in the event of power failure Emergency shutdown In an emergency situation do as follows to stop the run Step Action al In the STORM Scanner Control click CANCEL SCAN to abort the scan 2 If required press the power switch to the OFF position to turn the instrument off Power failure The result of a power failure depends on which unit that is affected Power failure to will result in Storm instrument The run is interrupted immediately in an undefined state The data collected up to the time of the power failure is available in the file created when starting the scan Computer The computer shuts down in an undefined state e Therun continues but data cannot be saved 20 Storm Operating Instructions 28 9623 52 AA Safety instructions 2 Recycling information 2 5 2 5 Recycling information The equipment shall be decontaminated befo
49. talic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text e g Power switch 1 2 Regulatory information This section lists the directives and standards that are fulfilled by the Storm system Manufacturing information Requirements Content Name and address of manufacturer GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 84 Uppsala Sweden Name and address of person Peter L wendahl GE Healthcare responsible for Technical File Bj rkgatan 30 SE 751 84 Uppsala Sweden Name and ID of notified body INTERTEK SEMKO AB NB 0413 Place and date of declaration Uppsala Sweden Nov 2009 Storm Operating Instructions 28 9623 52 AA Introduction 1 Regulatory information 1 2 Requirements Content Identity of person authorized to sign See EC Declaration of Conformity Declaration of Conformity CE Conformity 2006 42 EC Machinery Directive MD 2006 95 EC Low Voltage Directive LVD 2004 108 EC ElectroMagnetic Compatibility EMC Directive International standards Standard Description Notes EN 61010 1 Safety requirements for electrical IEC 61010 1 equipment for measurement control UL 61010 1 CAN and laboratory use CSA C22 2 no 61010 1 EN 61326 1 EMC emissions and immunity Harmonized with requirements for measurement 2004 108 EC control and laboratory use EN ISO
50. the sample 0 c Place the edge of the screen into the groove E located along the side of the Exposure cassette opposite the clampis d Gently lower the screen into place in the corner guides F making sure the screen fits into the guides correctly Close the Exposure cassette by rotating each clamp counterclockwise until it stops at the pin and then flipping the lever over to lock the screen into place L Storm Operating Instructions 28 9623 52 AA 35 4 Operation 4 5 Preparations before start 6 Place an unmounted screen A in an Exposure cassette d The white side of the screen should face down toward the sample b Gently lower the screen into place over the grid area in the cassette The screen should be centered over the grid area and should lie straight and flat in the cassette c Close the Exposure cassette and press the lid shut until the lock clicks 0 Sample Storage phosphor screen exposure Note This section is valid only when performing Storage phosphor screen autoradiography NOTICE Condensation can destroy the screen If you are exposing a screen to a frozen sample place the screen in a sealed dry environment such as a sealed bag After exposure allow the screen to come to room temperature before removing it from the bag and scanning 1 Perform Storage phosphor screen autoradiography at room temperature 2 Estimate approxi
51. trong beta or gamma emitters or x ray machines e Ina protective box or in a clean Exposure cassette Damaged screens unusable and must be replaced g Check the Exposure cassette for contamination and clean the Exposure cassette Section 5 5 Exposure cassette on page 55 Analyzing or preprocessing the scanned image Open the image of the sample to determine the quality of the scan using an analyzing software Storm Operating Instructions 28 9623 52 AA 49 4 Operation 4 5 Procedures after scanning Turn off instrument and STORM Scanner Control 1 Select File Exit to exit the STORM Scanner Control If you want to scan again start the STORM Scanner Control as described Section 4 2 Starting the instrument and STORM Scanner Control on page 28 2 Pressthe Power switch on the lower left side of the Storm instrument to the off position to turn off the Storm instrument Note You can continue to use the computer after you turn off the Storm instrument However if you want to use the instrument again you must follow the procedures described in Section 4 2 Starting the instrument and STORM Scanner Control on page 28 3 Save and close any open files in the computer 4 Close all the running applications in the computer 5 Turn off the computer 6 Turn off any optional peripheral used according to the manufacturers instructions 50 Storm Operating Instructions 28 9623 52 AA Maintenance 5 General 5 1 5 Maintenance
52. unprotected surface of the screen NOTICE To avoid damaging the TR screen do not expose the screen to liquids of any sort 5 5 Exposure cassette To protect the Exposure cassettes from contamination and damage observe the following precautions and cleaning procedure e Do not place uncovered wet gels in the Exposure cassette Wet gels can permanently contaminate the cassette For information on using wet gels see Sample recommendations on page 28 e Donot place sharp or heavy objects inside the cassette A crease or dent in the cassette lining causes uneven pressure on the sample e Keep the foam inside the cassette dry e Immediately before placing the sample in the cassette clean the grid surface of the cassette with a damp cloth moistened with a solution of totally rinsable laboratory detergent equipment and damage it Use damp cloth only do not pour or spray NOTICE Use liquid sparingly when cleaning excess liquid may leak into the liquid onto the equipment 5 6 Image eraser The surface of the Image eraser must be clean and free of radioactive contamination when you erase screens Otherwise the screens will be exposed to radioactive contamination during the erasure process To clean the Image eraser follow the instruction below WARNING Because the Image eraser uses high voltage always turn off and unplug the Image eraser before cleaning the surface 1 Turn off the Image e
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